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WO2015019253A3 - Anti-dengue virus genetic vaccine based on the envelope protein ectodomains - Google Patents

Anti-dengue virus genetic vaccine based on the envelope protein ectodomains Download PDF

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Publication number
WO2015019253A3
WO2015019253A3 PCT/IB2014/063588 IB2014063588W WO2015019253A3 WO 2015019253 A3 WO2015019253 A3 WO 2015019253A3 IB 2014063588 W IB2014063588 W IB 2014063588W WO 2015019253 A3 WO2015019253 A3 WO 2015019253A3
Authority
WO
WIPO (PCT)
Prior art keywords
dengue virus
envelope protein
genetic vaccine
vaccine based
domain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2014/063588
Other languages
French (fr)
Other versions
WO2015019253A2 (en
Inventor
Oscar Burrone
Marco Bestagno
Monica POGGIANELLA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
International Centre for Genetic Engineering and Biotechnology
Original Assignee
International Centre for Genetic Engineering and Biotechnology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by International Centre for Genetic Engineering and Biotechnology filed Critical International Centre for Genetic Engineering and Biotechnology
Publication of WO2015019253A2 publication Critical patent/WO2015019253A2/en
Publication of WO2015019253A3 publication Critical patent/WO2015019253A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53—DNA (RNA) vaccination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/545—Medicinal preparations containing antigens or antibodies characterised by the dose, timing or administration schedule
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/70—Multivalent vaccine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
    • C12N2770/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
    • C12N2770/24134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The present invention refers to a genetic vaccine for dengue virus of all serotypes or for any virus belonging to the Flavivirus genus. Said vaccine is based on the envelope protein E ectodomains, more in particular on the DIII domain of dengue virus or of other Flavivirus. A genetic construct encoding a fusion protein wherein said domain of envelope protein E is fused to a carboxyterminal domain from the constant region of an immunoglobulin is disclosed in the present invention. The use of said construct allows for an efficient secretion of the fusion protein from the cells and thus for a high antibody response.
PCT/IB2014/063588 2013-08-05 2014-07-31 Anti-dengue virus genetic vaccine based on the envelope protein ectodomains Ceased WO2015019253A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITRM2013A000458 2013-08-05
IT000458A ITRM20130458A1 (en) 2013-08-05 2013-08-05 ANTI-DENGUE VIRUS GENETIC VACCINE BASED ON THE ECTODOMINS OF THE PROTEIN ENVELOPE

Publications (2)

Publication Number Publication Date
WO2015019253A2 WO2015019253A2 (en) 2015-02-12
WO2015019253A3 true WO2015019253A3 (en) 2015-05-28

Family

ID=49304201

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2014/063588 Ceased WO2015019253A2 (en) 2013-08-05 2014-07-31 Anti-dengue virus genetic vaccine based on the envelope protein ectodomains

Country Status (2)

Country Link
IT (1) ITRM20130458A1 (en)
WO (1) WO2015019253A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190022210A1 (en) * 2015-11-06 2019-01-24 Victor G. Solodushko Novel platform dna vaccine
US11666649B2 (en) 2016-10-11 2023-06-06 University Of Miami Vectors and vaccine cells for immunity against Zika virus
CN108503696B (en) * 2017-02-27 2023-05-12 中国科学院上海巴斯德研究所 A Zika virus subunit vaccine expressed in yeast cells
US20220289796A1 (en) * 2019-08-06 2022-09-15 The University Of North Carolina At Chapel Hill Methods and compositions for stabilized recombinant flavivirus e protein dimers

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040234951A1 (en) * 2001-07-16 2004-11-25 Lisset Hermida Cruz Chimeric chains that coding for proteins that induce effects directed against viruses
WO2009099716A1 (en) * 2008-01-11 2009-08-13 Vgx Pharmaceuticals, Inc. Novel vaccines against multiple subtypes of dengue virus
WO2011115583A1 (en) * 2010-03-19 2011-09-22 National University Of Singapore A novel platform for retrovirus-like particle (vlp)-display of vaccine antigens

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6455509B1 (en) 1996-06-04 2002-09-24 The United States Of America As Represented By The Secretary Of The Navy Dengue nucleic acid vaccines that induce neutralizing antibodies
WO2007034507A2 (en) 2005-09-20 2007-03-29 International Centre For Genetic Engineering And Biotechnology Tetravalent dengue specific domain iii based chimeric recombinant protein
US7981431B2 (en) 2007-06-08 2011-07-19 National Health Research Institutes Consensus dengue virus envelope protein domain III polypeptides (cED III) and their methods of use
WO2008152652A2 (en) 2007-06-12 2008-12-18 International Centre For Genetic Engineering And Biotechnology A dengue envelope domain iii-based tetravalent protein vaccine
CA2740598C (en) 2008-11-17 2018-05-22 Mathura P. Ramanathan Antigens that elicit immune response against flavivirus and methods of using same
BRPI0904020B8 (en) 2009-10-01 2021-05-25 Fundacao Oswaldo Cruz vaccine composition against the dengue virus, and kit
MX2012012681A (en) 2010-05-21 2012-12-17 Univ Pittsburgh Universal dengue virus sequences and methods of use.
CA2812306A1 (en) 2010-10-01 2012-04-05 University Of Rochester Flavivirus domain iii vaccine

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040234951A1 (en) * 2001-07-16 2004-11-25 Lisset Hermida Cruz Chimeric chains that coding for proteins that induce effects directed against viruses
WO2009099716A1 (en) * 2008-01-11 2009-08-13 Vgx Pharmaceuticals, Inc. Novel vaccines against multiple subtypes of dengue virus
WO2011115583A1 (en) * 2010-03-19 2011-09-22 National University Of Singapore A novel platform for retrovirus-like particle (vlp)-display of vaccine antigens

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHEN YAPING ET AL: "Demonstration of binding of dengue virus envelope protein to target cells", JOURNAL OF VIROLOGY, vol. 70, no. 12, 1996, pages 8765 - 8772, XP002734187, ISSN: 0022-538X *
LI G ET AL: "Comparison of immune responses against foot-and-mouth disease virus induced by fusion proteins using the swine IgG heavy chain constant region or beta-galactosidase as a carrier of immunogenic epitopes", VIROLOGY, ELSEVIER, AMSTERDAM, NL, vol. 3281-3, 5-12, 17, 18, 20-22, no. 2, 25 October 2004 (2004-10-25), pages 274 - 281, XP004581380, ISSN: 0042-6822, DOI: 10.1016/J.VIROL.2004.07.025 *
PURDY D E ET AL: "Secretion of noninfectious dengue virus-like particles and identification of amino acids in the stem region involved in intracellular retention of envelope protein", VIROLOGY, ELSEVIER, AMSTERDAM, NL, vol. 333, no. 2, 15 March 2005 (2005-03-15), pages 239 - 250, XP004757140, ISSN: 0042-6822, DOI: 10.1016/J.VIROL.2004.12.036 *
SARAH MURRELL ET AL: "Review of dengue virus and the development of a vaccine", BIOTECHNOLOGY ADVANCES, ELSEVIER PUBLISHING, BARKING, GB, vol. 29, no. 2, 29 November 2010 (2010-11-29), pages 239 - 247, XP028136136, ISSN: 0734-9750, [retrieved on 20101210], DOI: 10.1016/J.BIOTECHADV.2010.11.008 *
STAROPOLI I ET AL: "Dengue virus envelope glycoprotein can be secreted from insect cells as a fusion with the maltose-binding protein", JOURNAL OF VIROLOGICAL METHODS, vol. 56, no. 2, 1996, pages 179 - 189, XP002716233, ISSN: 0166-0934 *

Also Published As

Publication number Publication date
ITRM20130458A1 (en) 2015-02-05
WO2015019253A2 (en) 2015-02-12

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