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WO2015012421A1 - Procédé de production de matériau fermenté en utilisant des souches complexes provenant du corps humain et composition cosmétique fonctionnelle contenant le matériau fermenté - Google Patents

Procédé de production de matériau fermenté en utilisant des souches complexes provenant du corps humain et composition cosmétique fonctionnelle contenant le matériau fermenté Download PDF

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Publication number
WO2015012421A1
WO2015012421A1 PCT/KR2013/006599 KR2013006599W WO2015012421A1 WO 2015012421 A1 WO2015012421 A1 WO 2015012421A1 KR 2013006599 W KR2013006599 W KR 2013006599W WO 2015012421 A1 WO2015012421 A1 WO 2015012421A1
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WIPO (PCT)
Prior art keywords
lactobacillus
lactobacillus rhamnosus
kccm11349p
kccm11254p
strain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2013/006599
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English (en)
Korean (ko)
Inventor
이현용
최우석
최운용
김지선
임혜원
김남영
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHEBAH BIOTECH Inc
Seowon University Institute of Industry Academy Collaboration
Original Assignee
SHEBAH BIOTECH Inc
Seowon University Institute of Industry Academy Collaboration
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Priority to KR1020147009394A priority Critical patent/KR101627850B1/ko
Priority to PCT/KR2013/006599 priority patent/WO2015012421A1/fr
Publication of WO2015012421A1 publication Critical patent/WO2015012421A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/82—Preparation or application process involves sonication or ultrasonication
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Definitions

  • the present invention provides a method for preparing fermented products using Lactobacillus rhamnosus HK-9 and Lactobacillus paracasei HS-05 complex lactic acid bacteria derived from the human body, and antioxidant and skin whitening efficacy It relates to a functional cosmetic composition having a.
  • Melanin is an important means of protecting the living body from harmful ultraviolet rays in sunlight. It is a phenolic polymer that is widely used in animals, plants, and microorganisms. It increases the viability of UV rays, drying, and extreme temperatures. It is known to form freckle, blotch and promote skin aging and to induce skin cancer of malignant melanoma. Moreover, melanin is a chemically and physically stable substance, and thus, once produced, pigments that inhibit the synthesis of melanin as a skin whitening agent as a method for preventing and treating them are almost impossible to be decomposed and removed in a short time without significant damage to the skin. I'm using.
  • Tyrosinase is a major enzyme in melanin biosynthesis and is a copper-containing enzyme that uses phenolic compounds as a substrate. Tyrosine is oxidized to L-3,4-dihydroxyl-L-phenylalanine (LDOPA) by tyrosinase and L-DOPA to dopaquinone, which in turn is 5,6-dihydroxy indole, indole 5 It is oxidized to 6-quinone and finally polymerized melanin by polymerization (Lopez et al., 1992).
  • LOPA L-3,4-dihydroxyl-L-phenylalanine
  • tyrosinase inhibitors include ascorbic acid, kojic acid (Loj et al., 2006), hydroquinone (Fitton and Goa, 1991; Parvez et al., 2006), benzoic acid (benzoic acid), retinoid (retinoid), arbutin (arbutin, Maeda and Fukuda, 1996), etc.
  • kojic acid and arbutin (arbutin) has a strong whitening effect, but problems such as product stability and economy Is difficult to use (Ando et al., 1993; Masuda et al., 1996).
  • the present inventors are Lactobacillus rhamnosus (Lactobacillus) that is derived from the human body ( Lactobacillus rhamnosus ) HK-9 KCCM11349P and Lactobacillus paracazei Lactobacillus paracasei )
  • Lactobacillus rhamnosus Lactobacillus rhamnosus
  • HK-9 KCCM11349P Lactobacillus paracazei Lactobacillus paracasei
  • an object of the present invention is Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) HK-9 KCCM11349P and / or Lactobacillus paracasei ( Lactobacillus paracasei ) It is to provide an antioxidant or skin whitening cosmetic composition containing the HS-05 KCCM11254P strain as an active ingredient.
  • Another object of the present invention is Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) HK-9 KCCM11349P and / or Lactobacillus paracasei ( Lactobacillus paracasei ) It is to provide an antioxidant or skin whitening cosmetic composition containing the hot water extract of HS-05 KCCM11254P strain as an active ingredient.
  • Another object of the present invention is Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) HK-9 KCCM11349P and / or Lactobacillus paracasei ( Lactobacillus paracasei ) It is to provide a health functional food containing HS-05 KCCM11254P strain as an active ingredient.
  • an object of the present invention is to provide a method for producing a cosmetic composition for antioxidant or skin whitening.
  • the present invention is Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) HK-9 KCCM11349P and / or Lactobacillus paracasei ( Lactobacillus paracasei )
  • a cosmetic composition for antioxidant or skin whitening containing HS-05 KCCM11254P strain as an active ingredient is provided.
  • the cosmetic composition is Lactobacillus rhamnosus (Scutellaria baicalensis GEORGE) Lactobacillus rhamnosus ) HK-9 KCCM11349P and / or Lactobacillus paracasei ( Lactobacillus paracasei ) After inoculating HS-05 KCCM11254P strain, it may be to exhibit an antioxidant or skin whitening effect.
  • the present invention is Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) HK-9 KCCM11349P and / or Lactobacillus paracasei ( Lactobacillus paracasei ) It provides an antioxidant or skin whitening cosmetic composition containing the hot water extract of HS-05 KCCM11254P strain as an active ingredient.
  • the present invention is Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) HK-9 KCCM11349P and / or Lactobacillus paracasei ( Lactobacillus paracasei ) It provides a health functional food containing HS-05 KCCM11254P strain as an active ingredient.
  • the present invention (a) Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) HK-9 KCCM11349P and / or Lactobacillus paracaze (La ctobacillus paracasei ) Crushing the strain by sonicating 100 ml of the HS-05 KCCM11254P strain at 50 to 60 kHz for 20 to 50 minutes; And (b) adding the strain crushed in step (a) and the crushed liquid to 500 ml to 1 L of water at 50 to 70 ° C., and extracting hot water for 12 to 24 hours. It provides a method of manufacturing.
  • the natural material of the step (a) is golden, bramble, green tea, green beans, sunflower seeds, magnolia flowers, peony root, cedar, chopsticks, pine needles, thyme, deodeok, bellflower, Astragalus , Canopy, leafy, quince, ginseng or wild ginseng.
  • the present invention provides a method for preparing fermented products using Lactobacillus rhamnosus HK-9 and Lactobacillus paracasei HS-05 complex lactic acid bacteria derived from human skin, and antioxidant and skin whitening containing the fermented products.
  • the present invention relates to a functional cosmetic composition having efficacy, wherein when the strains are fermented in combination, they show higher antioxidant and skin whitening activity than when the strains are fermented, respectively, and elutes more bioactive substances and active substances. In addition, it can bring the maximum effect, which is advantageous economically.
  • 1 is a graph measuring the degree of strain culture over time of Lactobacillus rhamnosus HK-9 KCCM11349P.
  • Figure 2 is a graph measuring the degree of strain culture over time of Lactobacillus paracasei HS-05 KCCM11254P.
  • Figure 3 is a graph measuring the degree of strain culture over time when Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P lactic acid bacteria complex culture.
  • Figure 4 is a Lactobacillus rhamnosus HK-9 KCCM11349P group, Lactobacillus paracasei HS-05 KCCM11254P group, a group cultured fermentation of the above two lactic acid bacteria of the present invention and a control group, the control of the radical scavenging Lactobacillus casei fermentation group as a general lactic acid bacteria A graph showing the results.
  • Figure 6 is measured Lactobacillus rhamnosus HK-9 KCCM11349P group, Lactobacillus paracasei HS-05 KCCM11254P group, a group cultured fermentation of the above two lactic acid bacteria of the present invention and a control group in the lactic acid bacteria Lactobacillus casei fermentation group as a control group to measure the melanin synthesis inhibition A graph showing one result.
  • Figure 7 shows the inhibition rate of tyrosinase in the Lactobacillus rhamnosus HK-9 KCCM11349P group, Lactobacillus paracasei HS-05 KCCM11254P group, a group cultured fermentation of the two lactic acid bacteria of the present invention and the Lactobacillus casei fermentation group of the general lactic acid bacteria as a control group It is a graph showing the result of the measurement.
  • the present invention provides a method for preparing fermented products using Lactobacillus rhamnosus HK-9 and Lactobacillus paracasei HS-05 complex lactic acid bacteria derived from human skin, and antioxidant and skin whitening containing the fermented products. It is characterized by providing a functional cosmetic composition having efficacy.
  • the present inventors paid attention to the lactic acid bacteria which were studied for the preparation of cosmetics for skin whitening having excellent stability with low cost and low cost.
  • Lactobacillus is an important microorganism widely used and applied worldwide, and has been used for a long time in fermented dairy products such as cheese, fermented milk and lactic acid bacteria beverage and food industry such as Kimchi and Cheonggukjang. Lactobacillus has a long-acting effect of treating and preventing intestinal diseases and prevents the growth of harmful bacteria in the intestine and creates good conditions for the growth of beneficial bacteria, preventing food poisoning, preventing intestinal rot, preventing constipation, inhibiting carcinogenesis, and increasing immunity. It is known to have various physiological activities such as, and is used in various dairy, food and cosmetic industries.
  • the inventors have noted that the woman's hand is softer and contains more lactic acid bacteria than the man's hand. (The influence of sex handedness and washing on the diversity of hand surface bacteria, PNAS 105 (46) 17994-17999)
  • the lactic acid bacteria present were identified and physiologically active, and two kinds of Lactobacillus bacteria were selected. Lactobacillus rhamnosus HK-9 KCCM11349P ”and“ Lactobacillus paracasei HS-05 KCCM11254P ”.
  • Lactobacillus rhamnosus HK-9 and Lactobacillus paracasei HS-05 strain of the present invention Lactobacillus rhamnosus HK-9 KCCM11349P group, Lactobacillus paracasei HS-05 KCCM11254P group in gold, and the group of the two lactic acid bacteria of the present invention in combination culture and fermentation group Lactobacillus casei fermentation group as a control group DPPH scavenging activity, reducing power and
  • the golden fermentation of Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P showed higher DPPH scavenging activity, reducing power and total phenolic content than those of normal lactic acid bacteria and the strains. It was found that it showed high antioxidant activity (see Table 1), and this effect was disrupted
  • the results of the fermentation of Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P in the golden lactic acid bacteria and the strains were respectively higher than those of fermenting melanin synthesis and tyrosine.
  • Lactobacillus rhamnosus HK-9 and Lactobacillus paracasei HS-05 complex lactic acid bacteria of the present invention can provide a functional cosmetic composition because it has excellent antioxidant and skin whitening effects. have.
  • the strain according to the present invention may be added in 0.001 to 20% by weight based on the total weight of the cosmetic composition.
  • Lactobacillus rhamnosus Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracazei Lactobacillus paracasei )
  • the HS-05 KCCM11254P complex strain is added in an amount less than 0.001% by weight relative to the total weight of the cosmetic, it does not exhibit various physiological activity effects of the cosmetic composition of the present invention, whereas when added in excess of 20% by weight, it is toxic to cells and the like. It is desirable to add the content within the above-described range in consideration of economics because it does not obtain the desired effect in proportion to the amount added, and also because the cosmetic composition price according to the amount added is not economical problem .
  • the composition of the present invention may not only ferment the above-mentioned complex strains, but may also include components conventionally used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers. , Conventional adjuvants such as vitamins, pigments and flavorings, and carriers.
  • the cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.
  • the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components.
  • animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components.
  • animal oils vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide
  • cellulose derivatives polyethylene glycols
  • silicones bentonites
  • silicas talc or zinc oxide
  • lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.
  • a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.
  • liquid carrier diluents such as water, ethanol or propylene glycol
  • suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.
  • the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide.
  • Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.
  • the present invention provides a cosmetic method characterized in that the cosmetic composition of the present invention is applied to human skin.
  • the cosmetic method of the present invention refers to all cosmetic methods for applying the cosmetic composition of the present invention to human skin. That is, all the methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.
  • the cosmetic composition of the present invention may be used alone or in duplicate, or may be used in combination with other cosmetic compositions other than the present invention.
  • the cosmetic composition excellent in improving the skin wrinkles according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.
  • the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation
  • the soap is liquid soap, powdered soap, solid soap and oil soap
  • the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towels and cleansing pack
  • the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.
  • the present inventors asked 40 men and women in their 20s and 40s to separate the lactic acid bacteria in the laboratory 7 days before the experiment, and the participants did not inform the contents of the experiment in advance so as not to perform sanitary treatment such as washing their hands before the experiment. The experiment was conducted.
  • the participants were inoculated with the initial microorganisms in the palms of the experiment participants in the prepared MRS agar medium, and colonies formed after the anaerobic culture at 30 ° C. for 24 hours were inoculated into the new MRS Agar medium with platinum to isolate the strains. .
  • Strains isolated from the second inoculation were subcultured to each new 10 ml MRS broth for each colony and fermented for each colony to select the two most active microorganisms.
  • microorganisms obtained by the above method were commissioned by the Korea Microorganism Conservation Center to identify lactic acid bacteria using PCR, and the microorganisms were deposited with an international patent organization.
  • the present inventors incubated Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P, two human skin-derived lactic acid bacteria isolated in Example 1, in an MRS agar medium to determine the optimal incubation time. , 1L distilled water, 55% MRS broth powder and 0.5% L-cysteine hydrochloride were added to the 2L flask to prepare a medium. The culture temperature of lactic acid bacteria was 36 °C, the experiment was carried out by anaerobic culture for 48 hours at a minimum stirring speed of 50 ⁇ 60rpm.
  • the number of bacteria was about 6.1 ⁇ 10 6 CFU / ml, Was increased to about 3.0 ⁇ 10 7 CFU / ml, and the population increased to a maximum of about 4.1 ⁇ 10 7 CFU / ml after 48 hours incubation, but gradually decreased after 48 hours incubation there was. Therefore, the optimum incubation time of the Lactobacillus paracasei HS-05 KCCM11254P strain of the present invention was also found to be 48 hours (see Fig. 2).
  • Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 The result of measuring the complex strains cultured degree according to KCCM11254P time, Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei 2 gaji strain for 12 hours compound incubation of HS-05 KCCM11254P
  • the number of bacteria was about 7.1 ⁇ 10 6 CFU / mL, and the number of bacteria increased greatly to about 4.2 ⁇ 10 7 CFU / mL after 24 hours of incubation, and the maximum was 4.7 ⁇ 10 when incubated for 48 hours.
  • Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P of the present invention was tested to determine whether there is an antioxidant activity enhancement effect.
  • Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P which are skin-derived lactic acid bacteria, were fermented, showing higher antioxidant activity, higher DPPH scavenging activity, reducing power and total phenolic content than normal lactic acid bacteria.
  • Combined fermentation with Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P showed up to 90% DPPH activity in Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P. I knew it was.
  • Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P, the skin-derived lactic acid bacteria of the present invention had a total phenol content that was about 2 times higher than that of lactic acid bacteria. Reference).
  • the present inventors measured the melanin inhibitory activity and tyrosinase inhibitory activity of Lactobacillus rhamnosus HK-9 KCCM11349P, Lactobacillus paracasei HS-05 KCCM11254P, and the combined culture fermentation of the two strains in order to examine the enhancement of whitening activity through natural product fermentation. .
  • the combined fermentation of Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P of the present invention is more effective than the lactic acid bacteria and Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P. Elution was easy and extraction efficiency was also good.
  • the inventors of the present invention Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P in combination culture to verify the efficacy of the antioxidant active material from the strain itself (Radical scavenging activity) and reducing power measurement experiments (Reducing power).
  • the antioxidant scavenging ability was measured through the ability to eliminate free radicals.
  • the radical scavenging rate was increased depending on the concentration of the debris of the complex culture strain derived from the skin.
  • concentration of strain lysate 100% (v / v) it can be seen that the radical scavenging ability to reach about 30% (see Figure 4).
  • Reducing power is an index for determining the antioxidant activity by reducing the oxidized material to the original material.
  • the absorbance values of all the skin-derived culture strains were higher than those of a single skin-derived lactobacillus culture strain, especially absorbance value of 1.473 at the concentration of 100% (v / v). It was found to show the highest antioxidant activity (see FIG. 5).
  • the present inventors cultured the Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P of the present invention to verify the efficacy of the whitening active substance from the strain itself, and then crushed the strain by the same method as in Experiment 3 Melanin synthesis inhibition activity and Tyrosinase inhibition experiment were conducted.
  • tyrosinase inhibition rate of single skin-derived strain lysates was up to 28%, but tyrosinase inhibition rate of complex strain culture lysates showed up to 35%.
  • tyrosinase inhibition rate of complex strain culture lysates showed up to 35%.
  • Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P of the present invention were incubated in combination, Lactobacillus rhamnosus HK-9 KCCM11349P and Lactobacillus paracasei HS-05 KCCM11254P exhibited the highest efficiency than whitening-related cosmetics. It is advantageous to use.

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Abstract

La présente invention concerne un procédé de production d'un matériau fermenté en utilisant des bactéries complexes de l'acide lactique provenant du corps humain, Lactobacillus rhamnosus HK-9 et Lactobacillus paracasei HS-05, et une composition cosmétique fonctionnelle contenant le matériau fermenté et ayant des activités antioxydantes et de blanchiment de la peau. L'extrait dans l'eau chaude des bactéries de l'acide lactique du corps humain, Lactobacillus rhamnosus HK-9 etLactobacillus paracasei HS-05, et le matériau fermenté de Scutellaria baicalensis en utilisant les souches a présenté de fortes activités antioxydantes et de blanchiment de la peau, et élue davantage de substances bioactives et de matériaux efficaces, fournissant ainsi l'effet maximal en utilisant même une petite quantité et étant ainsi économiquement avantageux.
PCT/KR2013/006599 2013-07-23 2013-07-23 Procédé de production de matériau fermenté en utilisant des souches complexes provenant du corps humain et composition cosmétique fonctionnelle contenant le matériau fermenté Ceased WO2015012421A1 (fr)

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KR1020147009394A KR101627850B1 (ko) 2013-07-23 2013-07-23 인체 유래 복합 균주를 이용한 발효물의 제조방법 및 상기 발효물을 함유한 기능성 화장료 조성물
PCT/KR2013/006599 WO2015012421A1 (fr) 2013-07-23 2013-07-23 Procédé de production de matériau fermenté en utilisant des souches complexes provenant du corps humain et composition cosmétique fonctionnelle contenant le matériau fermenté

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PCT/KR2013/006599 WO2015012421A1 (fr) 2013-07-23 2013-07-23 Procédé de production de matériau fermenté en utilisant des souches complexes provenant du corps humain et composition cosmétique fonctionnelle contenant le matériau fermenté

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20120061733A (ko) * 2010-10-25 2012-06-13 (주)아모레퍼시픽 녹차 추출물을 함유하는 미백용 화장료 조성물
WO2019149940A1 (fr) * 2018-02-02 2019-08-08 Postbiotica S.R.L. Utilisation d'une composition à base de postbiotique pour le traitement de maladies de la peau
WO2020127581A1 (fr) * 2018-12-21 2020-06-25 L V M H Recherche Composition comprenant un extrait de lactobacillus rhamnosus
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KR20120061733A (ko) * 2010-10-25 2012-06-13 (주)아모레퍼시픽 녹차 추출물을 함유하는 미백용 화장료 조성물
KR101951782B1 (ko) 2010-10-25 2019-02-25 (주)아모레퍼시픽 녹차 추출물을 함유하는 미백용 화장료 조성물
WO2019149940A1 (fr) * 2018-02-02 2019-08-08 Postbiotica S.R.L. Utilisation d'une composition à base de postbiotique pour le traitement de maladies de la peau
US12324820B2 (en) 2018-02-02 2025-06-10 Postbiotica S.R.L. Use of a postbiotic-based composition for the treatment of skin diseases
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FR3090383A1 (fr) * 2018-12-21 2020-06-26 L V M H Recherche Composition comprenant un extrait de Lactobacillus rhamnosus
CN113453660B (zh) * 2018-12-21 2024-05-03 Lvmh研究公司 包含鼠李糖乳杆菌提取物的组合物
US12011501B2 (en) 2018-12-21 2024-06-18 L V M H Recherche Composition comprising a lactobacillus rhamnosus extract
WO2020127581A1 (fr) * 2018-12-21 2020-06-25 L V M H Recherche Composition comprenant un extrait de lactobacillus rhamnosus
CN112702990A (zh) * 2019-04-26 2021-04-23 乐土美森有限公司 包含新型鼠李糖乳杆菌lm1011作为有效成分的皮肤美白用化妆品组合物
US20220211610A1 (en) * 2019-04-26 2022-07-07 Lactomason Co., Ltd. Skin whitening cosmetic composition comprising lactobacillus rhamnosus lm1011 having immunostimulating activity
CN112702990B (zh) * 2019-04-26 2023-06-02 乐土美森有限公司 包含新型鼠李糖乳杆菌lm1011作为有效成分的皮肤美白用化妆品组合物
CN115786404A (zh) * 2021-10-22 2023-03-14 深圳市肽素生物技术有限公司 一种肽素、肽素精华物及在治疗皮肤切割伤药物方面的应用
CN114452241A (zh) * 2022-03-16 2022-05-10 渤海大学 一种益生菌小分子面膜及其制备方法
CN115836997A (zh) * 2022-11-02 2023-03-24 菏泽市亿鑫生物科技有限公司 一种绿豆籽发酵物，含其皮肤外用剂及其制备方法和应用

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