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WO2015095359A1 - Méthodes de détection de maladies ou d'états pathologiques - Google Patents

Méthodes de détection de maladies ou d'états pathologiques Download PDF

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WO2015095359A1
WO2015095359A1 PCT/US2014/070907 US2014070907W WO2015095359A1 WO 2015095359 A1 WO2015095359 A1 WO 2015095359A1 US 2014070907 W US2014070907 W US 2014070907W WO 2015095359 A1 WO2015095359 A1 WO 2015095359A1
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isolated
cells
population
disease
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Harry Stylli
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

Definitions

  • This invention relates generally to methods of using combinations of two or more different components selected from cell-free bodily fluids, phagocytic cells, circulating vesicles, and circulating diseased cells in the diagnosis, prognosis, or monitoring of a disease or condition.
  • the invention also relates to methods of using the combinations to identify markers of diseases or conditions.
  • Leukocytes begin as pluripotent hematopoietic stem cells in the bone marrow and develop along either the myeloid lineage (monocytes, macrophages, neutrophils, eosinophils, and basophils) or the lymphoid lineage (T and B).
  • myeloid lineage monocytes, macrophages, neutrophils, eosinophils, and basophils
  • lymphoid lineage T and B
  • One object of the present invention is to provide diagnostic methods that can facilitate the detection of a disease or condition-specific markers, e.g., nucleic acids, proteins, carbohydrates, and/or lipids and the like by using combinations of two or more different components selected from cell-free bodily fluids, phagocytic cells, circulating vesicles, and circulating diseased cells.
  • Another object of this invention is to provide methods of identifying a disease or condition-specific markers and further use such markers alone or together with any known markers to diagnose diseases or conditions.
  • a method for identifying a compound capable of ameliorating or treating a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a first sample comprising two or more different components selected from the group consisting of: a cell-free bodily fluid isolated from the subject before administering the compound to the subject, a population of phagocytic cells isolated from the subject before administering the compound to the subject, a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject before administering the compound to the subject, a population of circulating vesicles isolated from the subject before administering the compound to the subject, and a population of circulating diseased cells isolated from the subject before administering the compound to the subject; determining a second profile of at least one of the one or more markers from a first control comprising a component selected from the group consisting of: a population of phagocytic cells having a DNA content of 2
  • a method for assessing the efficacy of a treatment for a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a first sample comprising two or more different components selected from the group consisting of: a cell-free bodily fluid isolated from the subject before the treatment, a population of phagocytic cells isolated from the subject before the treatment, a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject before the treatment, a population of circulating vesicles isolated from the subject before the treatment, and a population of circulating diseased cells isolated from the subject before the treatment; b) determining a second profile of one or more markers of the disease or condition from a second sample comprising two or more different components selected from the group consisting of: a cell-free bodily fluid isolated from the subject after the treatment, a population of phagocytic cells isolated from the subject after the treatment, a population
  • a method for diagnosing or aiding in the diagnosis of a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a sample comprising components selected from the group consisting of: an analyte isolated from a cell-free bodily fluid isolated from the subject, an analyte isolated from a population of phagocytic cells isolated from the subject, an analyte isolated from a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject, an analyte isolated from a population of circulating vesicles isolated from the subject, and an analyte isolated from a population of circulating diseased cells isolated from the subject; b) determining a second profile of at least one of the one or more markers from a control comprising a component selected from the group consisting of: an analyte isolated from a population of phagocytic cells having a DNA
  • a method for prognosing or aiding in the prognosis of a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a sample comprising two or more different components selected from the group consisting of: an analyte isolated from a cell-free bodily fluid isolated from the subject, an analyte isolated from a population of phagocytic cells isolated from the subject, an analyte isolated from a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject, an analyte isolated from a population of circulating vesicles isolated from the subject, and an analyte isolated from a population of circulating diseased cells isolated from the subject; b) determining a second profile of at least one of the one or more markers from a control comprising a component selected from the group consisting of: an analyte isolated from a population of phag
  • a method for assessing the efficacy of a treatment for a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a first sample comprising two or more different components selected from the group consisting of: an analyte isolated from a cell-free bodily fluid isolated from the subject before the treatment, an analyte isolated from a population of phagocytic cells isolated from the subject before the treatment, an analyte isolated from a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject before the treatment, an analyte isolated from a population of circulating vesicles isolated from the subject before the treatment, and an analyte isolated from a population of circulating diseased cells isolated from the subject before the treatment; determining a second profile of at least one of the one or more markers from a first control comprising a component selected from the group consisting of: an ana
  • a method for monitoring the progression or regression of a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a first sample comprising two or more different components selected from the group consisting of: an analyte isolated from a cell-free bodily fluid isolated from the subject at a first time point, an analyte isolated from a population of phagocytic cells isolated from the subject at a first time point, an analyte isolated from a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject at a first time point, an analyte isolated from a population of circulating vesicles isolated from the subject at a first time point, and an analyte isolated from a population of circulating diseased cells isolated from the subject at a first time point; determining a second profile of at least one of the one or more markers from a first control comprising a component selected
  • a method for identifying a compound capable of ameliorating or treating a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a first sample comprising two or more different components selected from the group consisting of: an analyte isolated from a cell-free bodily fluid isolated from the subject before administering the compound to the subject, an analyte isolated from a population of phagocytic cells isolated from the subject before administering the compound to the subject, an analyte isolated from a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject before administering the compound to the subject, an analyte isolated from a population of circulating vesicles isolated from the subject before administering the compound to the subject, and an analyte isolated from a population of circulating diseased cells isolated from the subject before administering the compound to the subject; determining a second profile of at least one of the
  • a method for assessing the efficacy of a treatment for a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a first sample comprising two or more different components selected from the group consisting of: an analyte isolated from a cell-free bodily fluid isolated from the subject before the treatment, a population of phagocytic cells isolated from the subject before the treatment, an analyte isolated from a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject before the treatment, an analyte isolated from a population of circulating vesicles isolated from the subject before the treatment, and an analyte isolated from a population of circulating diseased cells isolated from the subject before the treatment; b) determining a second profile of one or more markers of the disease or condition from a second sample comprising two or more different components selected from the group consisting of: an analyte
  • a method for identifying a compound capable of ameliorating or treating a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a first sample comprising two or more different components selected from the group consisting of: an analyte isolated from a cell-free bodily fluid isolated from the subject before administering the compound to the subject, an analyte isolated from a population of phagocytic cells isolated from the subject before administering the compound to the subject, an analyte isolated from a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject before administering the compound to the subject, an analyte isolated from a population of circulating vesicles isolated from the subject before administering the compound to the subject, and an analyte isolated from a population of circulating diseased cells isolated from the subject before administering the compound to the subject; b) determining a second profile of one or
  • a method for diagnosing or aiding in the diagnosis of a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a sample comprising two or more different components selected from the group consisting of: a cell-free bodily fluid isolated from the subject, a population of phagocytic cells isolated from the subject, a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject, a population of circulating vesicles isolated from the subject, and a population of circulating diseased cells isolated from the subject; and b) identifying a difference between the first profile and a second profile of at least one of the one or more markers from a repository of said markers of said disease or condition, wherein the difference is indicative of the presence of said disease or condition in the subject.
  • a method for assessing the risk of developing a disease or condition in a subj ect comprising : a) determining a first profile of one or more markers of the disease or condition from a sample comprising two or more different components selected from the group consisting of: a cell-free bodily fluid isolated from the subject, a population of phagocytic cells isolated from the subject, a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject, a population of circulating vesicles isolated from the subject, and a population of circulating diseased cells isolated from the subject; and b) identifying a difference between the first profile and a second profile of at least one of the one or more markers from a repository of said markers of said disease or condition , wherein the difference is indicative of the risk of developing said disease or condition in the subject.
  • a method for prognosing or aiding in the prognosis of a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a sample comprising two or more different components selected from the group consisting of: a cell-free bodily fluid isolated from the subject, a population of phagocytic cells isolated from the subject, a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject, a population of circulating vesicles isolated from the subject, and a population of circulating diseased cells isolated from the subject; and b) identifying a difference between the first profile and a second profile of at least one of the one or more markers from a repository of said markers of said disease or condition, wherein the difference is indicative of the prognosis of said disease or condition in the subject.
  • a method for diagnosing or aiding in the diagnosis of a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a sample comprising components selected from the group consisting of: an analyte isolated from a cell- free bodily fluid isolated from the subject, an analyte isolated from a population of phagocytic cells isolated from the subject, an analyte isolated from a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject, an analyte isolated from a population of circulating vesicles isolated from the subject, and an analyte isolated from a population of circulating diseased cells isolated from the subject; and b) identifying a difference between the first profile and a second profile of at least one of the one or more markers from a repository of said markers of said disease or condition , wherein the difference is indicative of the presence of said disease or condition in the
  • a method for assessing the risk of developing a disease or condition in a subject comprising: a) determining a first profile of one or more markers of the disease or condition from a sample comprising two or more different components selected from the group consisting of: an analyte isolated from a cell-free bodily fluid isolated from the subject, an analyte isolated from a population of phagocytic cells isolated from the subject, an analyte isolated from a population of phagocytic cells having a DNA content more than 2n (>2n phagocytic cells) isolated from the subject, an analyte isolated from a population of circulating vesicles isolated from the subject, and an analyte isolated from a population of circulating diseased cells isolated from the subject; and b) identifying a difference between the first profile and a second profile of at least one of the one or more markers from a repository of said markers of said disease or condition , wherein the difference is indicative of the risk of developing said disease or condition
  • non-phagocytic cells are T cells, B cells, null cells, basophils, or mixtures thereof.
  • the circulating diseased cells are blood cells, tumor cells, lymphoma cells, fetal cells, apoptotic cells, epithelia cells, endothelial cells, stem cells, progenitor cells, mesenchymal cells, osteoblast cells, osteocytes, hematopoietic stem cells, foam cells, adipose cells, transcervical cells, circulating cardiocytes, circulating fibrocytes, circulating myocytes, circulating cells from kidney, circulating cells from gastrointestinal tract, circulating cells from lung, circulating cells from reproductive organs, circulating cells from central nervous system, circulating hepatic cells, circulating cells from spleen, circulating cells from thymus, circulating cells from thyroid , circulating cells from an endocrine gland, circulating cells from parathyroid, circulating cells from pituitary, circulating cells from adrenal gland, circulating cells from islets of Langerhans, circulating cells from pancreas,
  • control cells are normal cells.
  • control cells are circulating cells.
  • circulating vesicles are selected from the group consisting of circulating microvesicles, apoptotic bodies, micro-particles, membrane-bound vesicles, multivesicular bodies, nanovesicles, microparticles, and AR DC-1 mediated microvesicles (ARMM).
  • the circulating microvesicles are exosomes or urinary exosomes.
  • nucleic acids are nucleotides, oligonucleotides, DNAs, R As, or DNA-R A hybrids.
  • DNAs are double-stranded DNAs, single-stranded DNAs, multi-stranded DNAs, complementary DNAs, genomic DNAs, or non-coding DNAs.
  • proteins are amino acids, peptides, enzymes, antigens, antibodies, cytokines, lipoproteins, glycoproteins, or hormones.
  • lipids are fatty acids, neutral fats, phosphatides, cholesterol, cholesterol esters, triglycerides, glycolipids, glycerolipids, glycerophospholipids, sphingolipids, sterol lipids, prenol lipids, saccharolipids, polyketides, choline glycerophospholipid, ethanolamine glycerophospholipid, phosphatidylinositol, phosphatidylglycerol, phosphatidylserine, lyso-choline glycerophospholipid, lyso-ethanolamine glycerophospholipid, phosphatidic acid, lyso- phosphatidic acid, sphingomyelin, galactosylceramide, glucosylceramide, free fatty acids, prostaglandins, triacylglycerol, diacylglycerol, monoacylglycerol, triacylglycerol, diacy
  • HDLs lipoproteins
  • sphingoid base- 1 -phosphates or derivatives thereof.
  • metabolites are primary metabolites, secondary metabolites, organic metabolites, inorganic metabolites, prostaglandins, hydroxyeicosatetraenoic acids, hydroxyoctadecadienoic acids, steroids, bile acids, vitamins, or derivatives thereof.
  • the profile is a nucleic acid profile, a protein profile, a lipid profile, a carbohydrate profile, a metabolite profile, or a combination thereof.
  • first profile or the second profile is a nucleic acid profile, a protein profile, a lipid profile, a carbohydrate profile, a metabolite profile, or a combination thereof.
  • nucleic acid profile is a genotypic profile, a single nucleotide polymorphism profile, a gene mutation profile, a gene copy number profile, a DNA methylation profile, a DNA acetylation profile, a chromosome dosage profile, a gene expression profile, or a combination thereof.
  • nucleic acid profile is determined by polymerase chain reaction (PCR) analysis, sequencing analysis, electrophoretic analysis, restriction fragment length polymorphism (RFLP) analysis, Northern blot analysis, quantitative PCR, reverse-transcriptase-PCR analysis (RT-PCR), allele- specific oligonucleotide hybridization analysis, comparative genomic hybridization, heteroduplex mobility assay (HMA), single strand conformational polymorphism (SSCP), denaturing gradient gel electrophisis (DGGE), RNAase mismatch analysis, mass spectrometry, tandem mass spectrometry, matrix assisted laser
  • PCR polymerase chain reaction
  • RFLP restriction fragment length polymorphism
  • RT-PCR reverse-transcriptase-PCR analysis
  • HMA heteroduplex mobility assay
  • SSCP single strand conformational polymorphism
  • DGGE denaturing gradient gel electrophisis
  • RNAase mismatch analysis mass spectrometry, tandem mass spectrometry, matrix assisted laser
  • MALDI-TOF desorption/ionization-time of flight
  • ESI electrospray ionization
  • SELDI-TOF surface-enhanced laser deorption/ionization-time of flight
  • Q-TOF quadrupole-time of flight
  • APPI-MS atmospheric pressure photoionization mass spectrometry
  • FTMS Fourier transform mass spectrometry
  • MALDI-FT-ICR desorption/ionization-Fourier transform-ion cyclotron resonance
  • SIMS secondary ion mass spectrometry
  • Southern blot analysis in situ hybridization, fluorescence in situ hybridization (FISH), chromogenic in situ hybridization (CISH), immunohistochemistry (IHC), microarray, comparative genomic hybridization, karyotyping, multiplex ligation-dependent probe amplification (MLPA), Quantitative Multiplex PCR of Short Fluorescent Fragments (QMPSF), microscopy, methylation specific PCR (MSP) assay, Hpall tiny fragment Enrichment by Ligation-mediated PCR (HELP) assay, radioactive acetate labeling assays, colorimetric DNA acetylation assay, chromatin immunoprecipitation combined with microarray (ChlP-on-chip) assay, restriction landmark genomic scanning, Methylated DNA immunoprecipitation (Me
  • FISH fluorescence in situ hybridization
  • nucleic acid profile is determined by a sequencing technique selected from the group consisting of targeted sequencing, single molecule real-time sequencing, exon sequencing, electron microscopy-based sequencing, transistor-mediated sequencing, direct sequencing, random shotgun sequencing, Sanger dideoxy termination sequencing, whole-genome sequencing, sequencing by hybridization, pyrosequencing, capillary electrophoresis, gel electrophoresis, duplex sequencing, cycle sequencing, single-base extension sequencing, solid-phase sequencing, high-throughput sequencing, massively parallel signature sequencing, emulsion PCR, co-amplification at lower denaturation temperature-PCR (COLD-PCR), multiplex PCR, sequencing by reversible dye terminator, paired-end sequencing, near-term sequencing, exonuclease sequencing, sequencing by ligation, short-read sequencing, single-molecule sequencing, sequencing-by-synthesis, real-time sequencing, reverse-terminator sequencing, nanopore sequencing, 454 sequencing, Solexa Genome Analyzer sequencing, SOLiD®
  • the protein profile is a protein expression profile, a protein activation profile, or a combination thereof.
  • the protein profile is determined by an immunohistochemistry assay, an enzyme-linked immunosorbent assay (ELISA), in situ hybridization, chromatography, liquid chromatography, size exclusion chromatography, high performance liquid chromatography (HPLC), gas
  • MALDI-TOF matrix assisted laser desorption/ionization-time of flight
  • ESI electrospray ionization
  • SELDI-TOF surface-enhanced laser deorption/ionization-time of flight
  • Q-TOF quadrupole-time of flight
  • APPI-MS atmospheric pressure photoionization mass spectrometry
  • FTMS Fourier transform mass spectrometry
  • HPAEC-PAD high performance anion exchange chromatography with pulsed amperometric detection
  • liquid chromatography gas chromatography
  • gas chromatography fluorescent assay
  • mass spectrometry tandem mass spectrometry
  • MALDI-TOF matrix assisted laser desorption/ionization-time of flight
  • ESI electrospray ionization
  • SELDI-TOF surface-enhanced laser deorption/ionization-time of flight
  • Q-TOF quadrupole-time of flight
  • APPI-MS atmospheric pressure photoionization mass spectrometry
  • FTMS Fourier transform mass spectrometry
  • MALDI-FT-ICR desorption/ionization-Fourier transform-ion cyclotron resonance
  • SIMS secondary ion mass spectrometry
  • radioimmunoassay microfiuidic chip-based assay, detection of fluorescence, detection of
  • the disease or condition is a cardiovascular disease or condition, a kidney-associated disease or condition, a prenatal or pregnancy-related disease or condition, a neurological or neuropsychiatric disease or condition, an autoimmune or immune-related disease or condition, a cancer, an infectious disease or condition, a mitochondrial disorder, a respiratory- gastrointestinal tract disease or condition, a reproductive disease or condition, an ophthalmic disease or condition, a musculo-skeletal disease or condition, or a dermal disease or condition.
  • the difference is greater than a 1-fold difference.
  • a method for identifying one or more markers for a disease or condition comprising: a) determining a first profile of analytes from a sample comprising a cell- free bodily fluid from a subject having said disease or condition and a population of phagocytic cells, or a population of >2n phagocytic cells, from a subject having said disease or condition; b) comparing the first profile to a second profile derived from a repository of analytes from a control subject not having said disease or condition; c) identifying a set of differences between the first and second profiles, wherein the set of differences is specific to the first profile relative to the second profile; and d) identifying one or more analytes specific to the set of differences, the identified analytes being markers of said disease or condition.
  • invention 95 further comprising: a) obtaining a fifth profile of analytes from cells or tissues affected by said disease or condition in the subject having said disease or condition; obtaining a sixth profile of analytes from cells or tissues not affected by said disease or condition in the subject having said disease or condition; identifying a set of differences between the fifth and sixth profiles, wherein the set of differences is specific to the fifth profile relative to the sixth profile; and b) identifying at least one of the one or more markers of c) present in the set of differences identified in d).
  • phagocytic cells or the >2n phagocytic cells comprise viable diseased cells, dead diseased cells, apoptotic diseased cells, circulating tumor cells, infectious agents, fetal cells, trophoblasts, or fragments thereof.
  • non- phagocytic cells are T cells, B cells, null cells, basophils, or mixtures thereof
  • the bodily fluid sample is blood, urine, stool, saliva, lymph fluid, cerebrospinal fluid, synovial fluid, cystic fluid, ascites, pleural effusion, fluid obtained from a pregnant woman in the first trimester, fluid obtained from a pregnant woman in the second trimester, fluid obtained from a pregnant woman in the third trimester, maternal blood, amniotic fluid, chorionic villus sample, fluid from a preimplantation embryo, maternal urine, maternal saliva, placental sample, fetal blood, lavage and cervical vaginal fluid, interstitial fluid, or ocular fluid.
  • micro fluidics micro fluidics, magnetic separation technique, fluorescent-magnetic separation technique, nanostructure, quantum dots, high throughput microscope-based platforms, or a combination thereof.
  • the cell-free bodily fluid sample is separated by using a substance present in the sample.
  • the one or more markers are nucleic acids, proteins, lipids, carbohydrates, metabolites, or
  • nucleic acids are nucleotides, oligonucleotides, DNAs, RNAs, or DNA-RNA hybrids.
  • RNAs are messenger RNAs (mRNAs), microRNAs (miRNAs), small nucleolar RNAs (snoRNAs), ribosomal RNAs (mRNAs), messenger RNAs (mRNAs), microRNAs (miRNAs), small nucleolar RNAs (snoRNAs), ribosomal RNAs (mRNAs), miRNAs, miRNAs, ribosomal
  • proteins are amino acids, peptides, enzymes, antigens, antibodies, cytokines, lipoproteins, glycoproteins, or hormones.
  • lipids are fatty acids, neutral fats, phosphatides, cholesterol, cholesterol esters, triglycerides, glycolipids, glycerolipids, glycerophospholipids, sphingolipids, sterol lipids, prenol lipids, saccharolipids, polyketides, choline glycerophospholipid, ethanolamine
  • metabolites are primary metabolites, secondary metabolites, organic metabolites, inorganic metabolites, prostaglandins, hydroxyeicosatetraenoic acids, hydroxyoctadecadienoic acids, steroids, bile acids, vitamins, or derivatives thereof.
  • any one of embodiments 90-118 wherein the profile is a nucleic acid profile, a protein profile, a lipid profile, a carbohydrate profile, a metabolite profile, or a combination thereof.
  • SELDI-TOF deorption/ionization-time of flight
  • Q-TOF quadrupole-time of flight
  • APPI-MS atmospheric pressure photoionization mass spectrometry
  • FTMS Fourier transform mass spectrometry
  • MALDI-FT-ICR matrix- assisted laser desorption/ionization-Fourier transform-ion cyclotron resonance
  • SIMS secondary ion mass spectrometry
  • PCR polymerase chain reaction
  • nucleic acid profile is a genotypic profile, a single nucleotide polymorphism profile, a gene mutation profile, a gene copy number profile, a DNA methylation profile, a DNA acetylation profile, a chromosome dosage profile, a gene expression profile, or a combination thereof.
  • nucleic acid profile is determined by polymerase chain reaction (PCR) analysis, sequencing analysis, electrophoretic analysis, restriction fragment length polymorphism (RFLP) analysis, Northern blot analysis, quantitative PCR, reverse-transcriptase-PCR analysis (RT- PCR), allele-specific oligonucleotide hybridization analysis, comparative genomic hybridization, heteroduplex mobility assay (HMA), single strand conformational polymorphism (SSCP), denaturing gradient gel electrophisis (DGGE), RNAase mismatch analysis, mass spectrometry, tandem mass spectrometry, matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, electrospray ionization (ESI) mass spectrometry, surface-enhanced laser
  • PCR polymerase chain reaction
  • RFLP restriction fragment length polymorphism
  • RT-PCR reverse-transcriptase-PCR analysis
  • DGGE denaturing gradient gel electrophisis
  • SELDI-TOF deorption/ionization-time of flight
  • Q-TOF quadrupole-time of flight
  • APPI-MS atmospheric pressure photoionization mass spectrometry
  • FTMS Fourier transform mass spectrometry
  • MALDI-FT-ICR matrix- assisted laser desorption/ionization-Fourier transform-ion cyclotron resonance
  • SIMS secondary ion mass spectrometry
  • Southern blot analysis in situ hybridization, fluorescence in situ hybridization (FISH), chromogenic in situ hybridization (CISH),
  • IHC immunohistochemistry
  • microarray comparative genomic hybridization, karyotyping, multiplex ligation-dependent probe amplification (MLPA), Quantitative Multiplex PCR of Short Fluorescent Fragments (QMPSF), microscopy, methylation specific PCR (MSP) assay, Hpall tiny fragment Enrichment by Ligation-mediated PCR (HELP) assay, radioactive acetate labeling assays, colorimetric DNA acetylation assay, chromatin immunoprecipitation combined with microarray (ChlP-on-chip) assay, restriction landmark genomic scanning, Methylated DNA immunoprecipitation (MeDIP), molecular break light assay for DNA adenine methyltransferase activity, chromatographic separation, methylation-sensitive restriction enzyme analysis, bisulfite-driven conversion of non-methylated cytosine to uracil, co-amplification at lower denaturation temperature-PCR (COLD-PCR), multiplex PCR, methyl-binding
  • MALDI-FT-ICR desorption/ionization-Fourier transform-ion cyclotron resonance
  • SIMS secondary ion mass spectrometry
  • radioimmunoassays microscopy, microfluidic chip-based assays, surface plasmon resonance, sequencing, Western blotting assay, or a combination thereof.
  • spectrometry tandem mass spectrometry, matrix assisted laser desorption/ionization- time of flight (MALDI-TOF) mass spectrometry, electrospray ionization (ESI) mass spectrometry, surface-enhanced laser deorption/ionization-time of flight (SELDI- TOF) mass spectrometry, quadrupole-time of flight (Q-TOF) mass spectrometry, atmospheric pressure photoionization mass spectrometry (APPI-MS), Fourier transform mass spectrometry (FTMS), matrix-assisted laser desorption/ionization- Fourier transform-ion cyclotron resonance (MALDI-FT-ICR) mass spectrometry, secondary ion mass spectrometry (SIMS), radioimmunoassays, microfluidic chip- based assay, detection of fluorescence, detection of chemiluminescence, or a combination thereof.
  • MALDI-TOF matrix assisted laser desorption/ionization- time of
  • HPAEC-PAD high performance anion exchange chromatography with pulsed amperometric detection
  • liquid chromatography gas chromatography
  • gas chromatography fluorescent assay
  • mass spectrometry tandem mass spectrometry
  • MALDI-TOF matrix assisted laser desorption/ionization-time of flight
  • ESI electrospray ionization
  • SELDI-TOF surface-enhanced laser deorption/ionization-time of flight
  • Q-TOF quadrupole-time of flight
  • APPI-MS atmospheric pressure photoionization mass spectrometry
  • FTMS Fourier transform mass spectrometry
  • MALDI-FT-ICR desorption/ionization-Fourier transform-ion cyclotron resonance
  • SIMS secondary ion mass spectrometry
  • radioimmunoassay micro fluidic chip-based assay, detection of fluorescence, detection of
  • the disease or condition is a cardiovascular disease or condition, a kidney-associated disease or condition, a prenatal or pregnancy-related disease or condition, a neurological or neuropsychiatric disease or condition, an autoimmune or immune-related disease or condition, a cancer, an infectious disease or condition, a mitochondrial disorder, a respiratory-gastrointestinal tract disease or condition, a reproductive disease or condition, an ophthalmic disease or condition, a musculo-skeletal disease or condition, or a dermal disease or condition.
  • the disease or condition is a cardiovascular disease or condition, a kidney-associated disease or condition, a prenatal or pregnancy-related disease or condition, a neurological or neuropsychiatric disease or condition, an autoimmune or immune-related disease or condition, a cancer, an infectious disease or condition, a mitochondrial disorder, a respiratory-gastrointestinal tract disease or condition, a reproductive disease or condition, an ophthalmic disease or condition, a musculo-skeletal disease or condition, or a dermal disease or condition.
  • the one or more markers comprise at least one gene selected from the group consisting of AKT2, BAK1, EGFR, ERBB2, ETS2, FOS, JUN, MAP2K1, MMP2, PDGFB, RBI, SERPINB2, SNCG, and SPP1.
  • the one or more markers comprise at least one gene selected from the group consisting of AKT1, AKT2, BAK2, CDC25A, E2F1, EGFR, ERBB2, FOS, JUN, MAP2K1, MMP2, NFKB1, PDGFB, PIK3R1, PNN, RBI, SERPINB2, SERPINB5, SNCG, SPP1, TERT, TIMP3, and TP53.
  • the one or more markers comprise at least one gene selected from the group consisting of AKT1, APAF1, ATM, CDC25A, CDKN1A, ETS2, FOS, IL8, ITGA4, ITGA6, ITGAV, JUN, MAP2K1 , NFKBIA, PLAU, PLAUR, RAF1 , SERPINB2, SYK, TIMP1, TNF, TNFRSF10B, and TNFRSF1A.
  • the one or more markers comprise at least one gene selected from the group consisting of ACP2, AK2, AKT3, ARL5B, ATP2B3, BGN, BRAF, BTG2, CAMKK2, CAPG, CAPN12, CPLX2, DENND5A, DNA2, FAM104A, FNIP1, GFRA4, GLUD1,
  • GNAQ GNAQ, GP1BB, HNRPLL, HOXA2, HPS3, INPP4A, ITGAV, KLHL23, LANCL2, LYPD6, MAPKAPK3, MEF2A (includes, EG:4205), MEF2C, NVL, PCYT1A, PGLYRP4, PLOD1, PPP1CB, PRKAB2, PROS1, PTPRE, RASA4 (includes,EG: 10156), RBMS2, RBPJ, STAT5B, THBS1, TRIB1, TRIM2, TSPAN6, and ZDHHC21.
  • the one or more markers comprise at least one gene selected from the group consisting of B4GALT5, BOPl, CCL2, CCL3, CCL3L1, CCRL2, CD83, CLEC4G, CLIC4, CTSC, CTSO, CXCL10, FCGR3A, FPR3, HBA1, HBB, LRMP, MAP1LC3B2, MS4A4A, MSRl, MYADML, NIDI, PF4, PION, RNF217, SAMD9L, SERPINGl, and SPARC.
  • PRKCB PRKCB, PSMB9, RCN3, RGS4, RNASE6, RTP4, SAMD9L, SEL1L, SERPINGl, SETX, SIGLEC10, SKIL, SLC7A7, SNORA21, SP100, SP110, SP140, SSFA2, STAT2, STK17B, STK3, TDRD7, TMCC1, TMPRSS11E2, TNFRSF1B, TPM1, TRIM21,TXNDC4, UBE2L6, UBE2W, USP18, VAV1, WARS, WIPF1, and WIPI1.
  • 144 the casedibene
  • RNASE6 RNASE6, RTP4
  • SAMD9L SEL1L
  • SERPINGl SERPINGl
  • SETX SIGLEC10
  • SKIL secretoridel
  • SLC7A7 secretoridel
  • SLC7A7 secretoridel
  • SNORA21 SP100
  • SP110 SP110
  • SP140 SP140
  • the one or more markers comprise at least one gene selected from the group consisting of ADAR, ADM, ALAS 1 , ANKRD22, ARHGAP27, B3GNT5, BCL10, C12orf35, C15orf29, C2orf59, CD177, CEACAM1, CPEB2, DDX58, F2RL1, GDPD3, GNAI3, HIST2H3A, HIST2H3D, HIST2H4A, HMGCR, HSPA6, HSPC159, IL4R, IMPA2, KPNB1, KREMEN1, KRT23, LDLR, LOC100130904, LTB4R, MAEA, MARK2,
  • a kit comprising a plurality of marker detection agents that detect at least one or more of the markers identified by the methods of any one of the embodiments 94-
  • a method of treating or preventing a disease or condition in a subject comprising administering to said subject a composition comprising a compound identified by the method of any one of embodiments 6 and 15.
  • 148. The method of any one of the embodiments 1-93 and 135-145, wherein the circulating diseased cells are infected by an infectious agent.
  • inherited condition or trait is selected from the group consisting of: sex, intelligence, eye color, hair color, skin tone, baldness, height, muscle type, or life expectancy
  • Figure 5 depicts a set of potentially informative markers and the relative percentage of reads of associated allelic frequencies in a combination sample of a circulating cell-free fluid (ccff) plus 1% monocyte sample. Along the x-axis are the markers and along the y-axis are the percent reads for the different alleles.
  • Triangle reference allele; circle: alternative allele.
  • Figure 8 depicts an exemplary informative marker in a maternal only sample and a combination sample.
  • Triangle reference allele
  • circle alternative allele.
  • Figure 11 depicts the calculated frequency of an allele (allele A) in combination samples of plasma and fetal cells.
  • a "patient”, “subject”, or “individual” are used interchangeably and refer to either a human or a non-human animal. These terms include mammals, such as humans, primates, livestock animals (e.g., bovines, porcines), companion animals (e.g., canines, felines) and rodents (e.g., mice and rats).
  • mammals such as humans, primates, livestock animals (e.g., bovines, porcines), companion animals (e.g., canines, felines) and rodents (e.g., mice and rats).
  • a control subject refers to any individual that has not been diagnosed as having the disease or condition being assayed.
  • the terms "normal control”, “healthy control”, and “not-diseased cells” likewise mean a sample (e.g., cells, serum, tissue) taken from a source (e.g., subject, control subject, cell line) that does not have the condition or disease being assayed and therefore may be used to determine the baseline for the condition or disorder being measured.
  • a source e.g., subject, control subject, cell line
  • the control subject, normal control, and healthy control include data obtained and used as a standard, i.e. it can be used over and over again for multiple different subjects.
  • PCT/USl 1/45009 PCT/USl 1/44969, PCT/USl 1/44973, PCT/USl 1/44991,
  • PCT/USl 1/45002, PCT/USl 1/44996, and PCT/USl 1/45018 are incorporated herein by reference for all purposes.
  • the methods of this invention have high specificity, sensitivity, and accuracy, and are capable of detecting disease or condition-specific markers present within a bodily fluid sample, cells or tissues.
  • the methods of this invention have improved specificity, sensitivity, and accuracy compared to current methods.
  • the methods of the invention also reduce the problem of losing valuable signal when, for example, a patient sample is collected and separated into individual components, of which only one is chosen for testing.
  • the invention provides non-invasive assays for the early detection of a disease or condition, i.e., before the disease can be diagnosed by conventional diagnostic techniques, e.g., imaging techniques, and, therefore, provide a foundation for improved decision-making relative to the needs and strategies for intervention, prevention, and treatment of individuals with such disease or condition.
  • the present invention provides methods for diagnosing or aiding in the diagnosis of a disease or condition by comparing profiles (e.g.,
  • One profile used for the comparison may be a profile from a sample comprising combinations of two or more different components (e.g., cell-free bodily fluids, phagocytic cells, circulating vesicles, fetal cells, and circulating diseased cells) from a subject, or from a sample comprising a combination of a cell-free bodily fluid and a phagocytic cell, or from a sample comprising a combination of a cell-free bodily fluid and a fetal cell.
  • two or more different components e.g., cell-free bodily fluids, phagocytic cells, circulating vesicles, fetal cells, and circulating diseased cells
  • a profile used for the comparison may be a profile from a sample comprising analytes isolated from, e.g., cell- free bodily fluids, phagocytic cells, phagocytic cells having a DNA content more than 2n (>2n phagocytic cells), circulating vesicles, fetal cells, and circulating diseased cells from a subject, or from a sample comprising analytes isolated from a cell free bodily fluid and analytes isolated from a phagocytic cell, or from a sample comprising analytes isolated from a cell free bodily fluid and analytes isolated from a fetal cell.
  • a sample comprising analytes from phagocytes and a cell-free bodily fluid could be generated by isolating analytes (e.g., nucleic acids or proteins) from phagocytes (e.g., by lysing the cells and using affinity-based techniques to isolate the analytes) and analytes (e.g., nucleic acids or proteins) from a cell-free bodily fluid, and combining the analytes to create a sample useful in creating a profile.
  • analytes e.g., nucleic acids or proteins
  • the combination sample comprises a lysate, or a fraction or portion of a lysate, of a population of phagocytic cells in combination with a cell-free bodily fluid. In certain embodiments, the combination sample comprises a lysate, or a fraction or portion of a lysate, of a population of fetal cells in combination with a cell-free bodily fluid.
  • the combination sample may comprise an analyte isolated from a lysate, or a fraction or portion of an analyte isolated from a lysate, of a population of phagocytic cells, a population of >2n phagocytic cells, a population of circulating vesicles, a population of fetal cells, or a population of circulating diseased cells in place of the population of phagocytic cells, the population of >2n phagocytic cells, the population of circulating vesicles, the population of fetal cells, or the population of circulating diseased cells, respectively.
  • the combination sample comprises an analyte isolated from a lysate, or a fraction or portion of an analyte isolated from a lysate, of a population of phagocytic cells in combination with a cell-free bodily fluid. In certain embodiments, the combination sample comprises an analyte isolated from a lysate, or a fraction or portion of an analyte isolated from a lysate, of a population of cells in combination with a cell-free bodily fluid.
  • the combination sample may comprise an analyte isolated from a cell- free bodily fluid, or a portion or fraction of an analyte isolated from a cell-free bodily fluid, rather than the cell-free bodily fluid itself.
  • the combination sample comprises an analyte isolated from a cell-free bodily fluid, or a portion or fraction of an analyte isolated form a cell-free bodily fluid, in combination with a phagocyte.
  • the combination sample comprises an analyte isolated from a cell-free bodily fluid, or a portion or fraction of an analyte isolated form a cell-free bodily fluid, in combination with a fetal cell.
  • control profile may be a profile from a repository of markers of a disease or condition.
  • control cells that are substantially free of cells affected by the disease or condition refers to a population of cells, as compared to circulating diseased cells, comprise significantly fewer amounts of cells affected by the disease or condition.
  • control cells that are substantially free of cells affected by the disease or condition are cells that are at least 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%), 99.5%), or 100% free of cells affected by the disease or condition.
  • control cells that can be used in the methods of this invention are substantially free of fetal material (e.g., nucleic acids, proteins, and any analyte described herein), such as control cells that are at least 75%, 80%>, 85%, 90%>, 91 >, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100% free of fetal material.
  • fetal material e.g., nucleic acids, proteins, and any analyte described herein
  • This invention also provides methods for assessing the risk of developing a disease or condition, prognosing said disease, monitoring said disease progression or regression, assessing the efficacy of a treatment, or identifying a compound capable of ameliorating or treating said disease or condition.
  • one or more components of whole blood that are substantially free of markers of a disease or condition may be isolated from a whole blood sample.
  • the one or more components of whole blood that are substantially free of markers may be used as a control sample (e.g., to determine a control profile), and the remaining portion of the whole blood sample may be used as an analytical sample (e.g., to determine an analytical profile).
  • a population of non-phagocytic cells may be isolated from a whole blood sample and used to determine a control profile, while the remaining portion of the whole blood sample is used to determine an analytical profile.
  • the methods of this invention can be used together with any known diagnostic methods, such as physical inspection, visual inspection, biopsy, scanning, histology, radiology, imaging, ultrasound, use of a commercial kit, genetic testing, immunological testing, analysis of bodily fluids, or monitoring neural activity.
  • diagnostic methods such as physical inspection, visual inspection, biopsy, scanning, histology, radiology, imaging, ultrasound, use of a commercial kit, genetic testing, immunological testing, analysis of bodily fluids, or monitoring neural activity.
  • Phagocytic cells that can be used in the methods of this invention include all types of cells that are capable of ingesting various types of substances (e.g., apoptotic cells, infectious agents, dead cells, viable cells, cell-free DNAs, cell-free RNAs, cell- free proteins).
  • the phagocytic cells are neutrophils, macrophages, monocytes, dendritic cells, foam cells, mast cells, eosinophils, or keratinocytes.
  • the phagocytic cells can be a mixture of different types of phagocytic cells.
  • the phagocytic cells can be activated phagocytic cells, e.g., activated macrophages or neutrophils.
  • a phagocyte is a histiocyte, e.g., a Langerhans cell.
  • >2n phagocytic cells refer to phagocytic cells that have a DNA content of greater than 2n
  • some phagocytic cells engulf live/dying/dead diseased cells (and sub-cellular fragments thereof) and/or cell-free disease-specific nucleic acids, proteins, carbohydrates and/or lipids present in bodily fluids. Such phagocytosis leads to the internalization of these disease markers into the phagocytic cell and, therefore, the DNA content of these phagocytic cells will become greater than 2n.
  • phagocytic cells have not engulfed living/dying/dead diseased cells or fragments and/or cell-free disease-specific nucleic acids, proteins, lipids, and/or carbohydrates present in bodily fluids.
  • the DNA contents of this group of phagocytic cells remain 2n.
  • the disease-specific markers e.g., DNA with disease-specific mutations
  • the mutated DNA of diseased cells is integrated into the normal DNA of the >2n phagocytic cells.
  • the internalized disease-specific markers are not expressed by the >2n phagocytic cells.
  • the markers may be translocated onto the membranes of the >2n phagocytic cells, or secreted out by the >2n phagocytic cells.
  • Circulating diseased cells that can be used in the methods of this invention include all types of circulating cells that may be affected by a disease or condition or infected by an infectious agent.
  • a circulating cell refers to a cell present in the bodily fluid.
  • a circulating cell may not necessarily circulate throughout the entire body or in the circulatory system.
  • a circulating cell may be present locally, such as in synovial fluid, or cerebrospinal fluid, or lymph fluid.
  • a circulating diseased cell may also be detached from a tissue or organ that has been affected by a disease or condition or infected by an infectious agent.
  • a circulating diseased cell is a cell that contains markers for an inherited condition or trait rather than a traditionally-defined "disease.”
  • the inherited condition or trait can include attributes such as, but not limited to, sex, intelligence, eye color, hair color, skin tone, baldness, height (e.g., adult height), muscle type, or life expectancy.
  • An inherited condition or trait may be a prenatal, neonatal, pediatric, or adult condition or trait.
  • an inherited condition or trait is detected using a diseased cell from a fetus, infant, child, or adult.
  • Control cells includes all types of normal cells, or healthy cells, or cells that are substantially free of a disease or condition, or cells that are substantially free of markers for an inherited condition or trait, or cells that are substantially free of an infectious agent.
  • Control cells may be circulating cells or non-circulating cells (e.g., biopsied cells) that are representative of a normal or non-diseased state to which measurements on circulating diseased cells are compared to determine whether one or more diseased-associated marker is present in different levels between the circulating diseased cells and the control cells.
  • the nature of the control cell may be a matter of design choice for a particular assay and may be derived or determined from normal tissue of the patient him- or herself.
  • the circulating diseased cells are blood cells, tumor cells, lymphoma cells, fetal cells, apoptotic cells, epithelia cells, endothelial cells, stem cells, progenitor cells, mesenchymal cells, osteoblast cells, osteocytes, hematopoietic stem cells, foam cells, adipose cells, transcervical cells, circulating cardiocytes, circulating fibrocytes, circulating cancer stem cells, circulating myocytes, circulating cells from kidney, circulating cells from gastrointestinal tract, circulating cells from lung, circulating cells from reproductive organs, circulating cells from central nervous system, circulating hepatic cells, circulating cells from spleen, circulating cells from thymus, circulating cells from thyroid , circulating cells from an endocrine gland, circulating cells from parathyroid, circulating cells from pituitary, circulating cells from adrenal gland, circulating cells from islets of Langerhans, circulating cells from pancreas,
  • the circulating diseased cells that can be used in the methods of this invention may be affected by various diseases or conditions.
  • the disease or condition may be affected by a circulating diseased cell (e.g., skin tone that is affected by melatonin-containing cells).
  • Cells may be enucleated, for example, by using physical removal (e.g., via microneedle, optical tweezer, or aspiration), chemical treatments, photoablation, or ultraviolet irradiation.
  • a "circulating vesicle” refers to a membrane -bound vesicle of cellular origin.
  • the circulating vesicle may not necessarily circulate throughout the entire body or in the circulatory system.
  • the circulating vesicle may be present locally, such as in synovial fluid, or cerebrospinal fluid, or lymph fluid.
  • the circulating vesicles are selected from the group consisting of circulating microvesicles, apoptotic bodies, micro-particles, membrane-bound vesicles, multivesicular bodies, nanovesicles, microparticles, and ARRDC-1 mediated microvesicles (ARMM).
  • the circulating microvesicles are exosomes or urinary exosomes.
  • a combination sample comprises two or more different components selected from the group consisting of: a cell-free bodily fluid isolated from a subject, a population of phagocytic cells isolated from the subject, a population of >2n phagocytic cells isolated from the subject, a population of circulating vesicles isolated from the subject, a population of fetal cells, and a population of circulating diseased cells isolated from the subject.
  • a combination sample comprises a cell-free bodily fluid isolated from a subject and a population of phagocytic cells isolated from the subject.
  • a combination sample comprises a cell-free bodily fluid isolated from a subject and a population of fetal cells isolated from the subject.
  • a combination sample comprises an analyte isolated from a cell-free bodily fluid isolated from the subject and an analyte isolated from a population of phagocytic cells isolated from the subject. In some embodiments, a combination sample comprises an analyte isolated from a cell-free bodily fluid isolated from the subject and an analyte isolated from a population of fetal cells isolated from the subject.
  • a "profile" of a marker of a disease or condition can broadly refer to any information concerning the marker. This information can be either qualitative (e.g., presence or absence) or quantitative (e.g., levels, copy numbers, or dosages). In some embodiments, a profile of a marker can indicate the absence of this marker.
  • the profile can be a nucleic acid (e.g., DNA or RNA) profile, a protein profile, a lipid profile, a carbohydrate profile, a metabolite profile, or a combination thereof.
  • a “marker” as used herein generally refers to an analyte which is
  • An analyte is differentially detectable if it can be distinguished
  • exemplary diseases or conditions are a cardiovascular disease or condition, a kidney-associated disease or condition, a prenatal or pregnancy-related disease or condition, a neurological or neuropsychiatric disease or condition, an autoimmune or immune-related disease or condition, a cancer, an infectious disease or condition, a pediatric disease, disorder, or condition, a mitochondrial disorder, a respiratory-gastrointestinal tract disease or condition, a reproductive disease or condition, an ophthalmic disease or condition, a musculo-skeletal disease or condition, or a dermal disease or condition.
  • cardiovascular disease or condition refers to any condition that affects systems of heart or blood vessels (arteries and veins).
  • cardiovascular diseases include, but are not limited to myocardial infarction, coronary artery disease, percutaneous transluminal coronary angioplasty (PTCA), coronary artery bypass surgery (CABG), restenosis, peripheral arterial disease, stroke, abdominal aorta aneurysm, intracranial aneurysm, large artery atherosclerotic stroke, cardiogenic stroke, an early onset myocardial infarction, heart failure, pulmonary embolism, acute coronary syndrome (ACS), angina, cardiac hypertrophy,
  • PTCA percutaneous transluminal coronary angioplasty
  • CABG coronary artery bypass surgery
  • restenosis peripheral arterial disease
  • stroke abdominal aorta aneurysm
  • intracranial aneurysm large artery atherosclerotic stroke
  • cardiogenic stroke an early onset myocardial infarction
  • heart failure pulmonary embolism
  • ACS acute coronary syndrome
  • angina cardiac hypertrophy
  • arteriosclerosis myocarditis, pancarditis, endocarditis, hypertension, congestive heart failure, atherosclerosis, cerebrovascular disease, declining cardiac health, ischemic heart disease, pericarditis, cardiogenic shock, alcoholic cardiomyopathy, congenital heart disease, ischemic cardiomyopathy, hypertensive cardiomyopathy, valvular cardiomyopathy, inflammatory cardiomyopathy, cardiomyopathy secondary to a systemic metabolic disease, dilated cardiomyopathy, hypertrophic cardiomyopathy, arrhythmogenic right ventricular cardiomyopathy, restrictive cardiomyopathy, noncompaction cardiomyopathy, valvular heart disease, hypertensive heart disease, myocardial ischemic attack, unstable angina, myocardial rupture, cardiogenic shock, embolism, deep vein thrombosis, arrhythmia, arrhythmogenic right ventricular cardiomyopathy, diabetic cardiomyopathy, mitral regurgitation, mitral valve prolapse, peripheral vascular disease, artery disease, carotid artery disease, deep vein thrombo
  • kidney-associated disease or condition refers to any disease or condition that affects kidney or renal system.
  • kidney-associated disease include, but are not limited to, chronic kidney diseases, primary kidney diseases, non-diabetic kidney diseases, glomerulonephritis, interstitial nephritis, diabetic kidney diseases, diabetic nephropathy, glomerulosclerosis, rapid progressive glomerulonephritis, renal fibrosis, Alport syndrome, IDDM nephritis, mesangial proliferative glomerulonephritis, membrano proliferative glomerulonephritis, crescentic glomerulonephritis, renal insterstitial fibrosis, focal segmental glomerulosclerosis, membranous nephropathy, minimal change disease, pauci-immune rapid progressive glomerulonephritis, IgA nephropathy, polycystic kidney disease, Den
  • myoglobinuria myoglobinuria, Wegener's Granulomatosis, Glycogen Storage Disease Type 1, chronic kidney disease, chronic renal failure, low Glomerular Filtration Rate (GFR), nephroangiosclerosis, lupus nephritis, ANCA-positive pauci-immune crescentic glomerulonephritis, chronic allograft nephropathy, nephrotoxicity, renal toxicity, kidney necrosis, kidney damage, glomerular and tubular injury, kidney dysfunction, nephritic syndrome, acute renal failure, chronic renal failure, proximal tubal dysfunction, acute kidney transplant rejection, chronic kidney transplant refection, non IgA mesangioproliferative glomerulonephritis, postinfectious glomerulonephritis, vasculitides with renal involvement of any kind, any hereditary renal disease, any interstitial nephritis, renal transplant failure, kidney cancer, kidney disease associated with other conditions (e.g
  • glomerulonephritis a bacterial endocarditis, a microscopic polyangitis, a Churg- Strauss syndrome, an anti-GBM-antibidy mediated glomerulonephritis, amyloidosis, a monoclonal immunoglobulin deposition disease, a fibrillary glomerulonephritis, an immunotactoid glomerulopathy, ischemic tubular injury, a medication-induced tubulo-interstitial nephritis, a toxic tubulo-interstitial nephritis, an infectious tubulo- interstitial nephritis, a bacterial pyelonephritis, a viral infectious tubulo-interstitial nephritis which results from a polyomavirus infection or an HIV infection, a metabolic-induced tubulo-interstitial disease, a mixed connective disease, a cast nephropathy, a crystal nephro
  • prenatal or pregnancy-related disease or condition refers to any disease, disorder, or condition affecting a pregnant woman, embryo, or fetus.
  • Prenatal or pregancy-related conditions can also refer to any disease, disorder, or condition that is associated with or arises, either directly or indirectly, as a result of pregnancy.
  • diseases or conditions can include any and all birth defects, congenital conditions, or hereditary diseases or conditions.
  • neurofibromatosis Alagille syndrome, Velocardiofacial syndrome, DiGeorge syndrome, steroid sulfatase deficiency, Prader-Willi syndrome, Kallmann syndrome, microphthalmia with linear skin defects, adrenal hypoplasia, glycerol kinase deficiency, Pelizaeus-Merzbacher disease, testis-determining factor on Y, azospermia (factor a), azospermia (factor b), azospermia (factor c), lp36 deletion,
  • phenylketonuria Tay-Sachs disease, adrenal hyperplasia, Fanconi anemia, spinal muscular atrophy, Duchenne's muscular dystrophy, Huntington's disease, myotonic dystrophy, Robertsonian translocation, Angelman syndrome, tuberous sclerosis, ataxia telangieltasia, open spina bifida, neural tube defects, ventral wall defects, small-for-gestational-age, congenital cytomegalovirus, achondroplasia, Marfan's syndrome, congenital hypothyroidism, congenital toxoplasmosis, biotinidase deficiency, galactosemia, maple syrup urine disease, homocystinuria, medium-chain acyl Co-A dehydrogenase deficiency, structural birth defects, heart defects, abnormal limbs, club foot, anencephaly, arhinencephaly/holoprosencephaly, hydrocephaly, anophthalmos/micro
  • neuroimmunological disorders neurootological disease, neurotrauma including spinal cord injury, pain including neuropathic pain, pediatric neurological and
  • an autoimmune or immune-related disease or condition refers to any disease or condition that affects the function of immune systems.
  • autoimmune or immune-related diseases or conditions include, but are not limited to, antiphospholipid syndrome, systemic lupus erythematosus, rheumatoid arthritis, autoimmune vasculitis, celiac disease, autoimmune thyroiditis, post-transfusion immunization, maternal-fetal incompatibility, transfusion reactions, immunological deficiency such IgA deficiency, common variable immunodeficiency, drug-induced lupus, diabetes mellitus, Type I diabetes, Type II diabetes, juvenile onset diabetes, juvenile rheumatoid arthritis, psoriatic arthritis, multiple sclerosis, immunodeficiency, allergies, asthma, psoriasis, atopic dermatitis, allergic contact dermatitis, chronic skin diseases, amyotrophic lateral sclerosis, chemotherapy-induced injury,
  • polyendocrinopathies Reiter's disease, stiff-man syndrome, giant cell arteritis, immune complex nephritis, IgA nephropathy, IgM polyneuropathies or IgM mediated neuropathy, idiopathic thrombocytopenic purpura (ITP), thrombotic throbocytopenic purpura (TTP), autoimmune thrombocytopenia, autoimmune disease of the testis and ovary including autoimmune orchitis and oophoritis, primary hypothyroidism, autoimmune endocrine diseases including autoimmune thyroiditis, chronic thyroiditis (Hashimoto's Thyroiditis), subacute thyroiditis, idiopathic hypothyroidism, Addison's disease, Grave's disease, autoimmune polyglandular syndromes (or polyglandular endocrinopathy syndromes), Sheehan's syndrome, autoimmune hepatitis, lymphoid interstitial pneumonitis (HIV), bronchi
  • cancer refers to various types of malignant neoplasms, most of which can invade surrounding tissues, and may metastasize to different sites (see, for example, PDR Medical Dictionary, 1st edition (1995), incorporated herein by reference in its entirety for all purposes).
  • neoplasm and “tumor” refer to an abnormal tissue that grows by cellular
  • abnormal tissue shows partial or complete lack of structural organization and functional coordination with the normal tissue which may be either benign (i.e., benign tumor) or malignant (i.e., malignant tumor).
  • carcinomas i.e., malignant tumors derived from epithelial cells such as, for example, common forms of breast, prostate, lung and colon cancer
  • sarcomas i.e., malignant tumors derived from connective tissue or mesenchymal cells
  • lymphomas i.e., malignancies derived from hematopoietic cells
  • leukemias i.e., malignancies derived from hematopoietic cells
  • germ cell tumors i.e., tumors derived from totipotent cells.
  • blastic tumors i.e., a typically malignant tumor which resembles an immature or embryonic tissue
  • an infectious disease or condition refers to any disease or condition that results from an infectious agent.
  • Infectious agents include, but are not limited to bacteria, viruses, fungi, protozoa, infectious proteins, parasitic microbes, and other parasites.
  • infectious diseases or conditions include, but are not limited to, bacterial infections, viral infections, fungal infections, protozoan infections, parasitic infections, hepatitis (e.g., hepatitis A, B, C, D, and E), herpes, influenza, human papillomavirus (HPV) infection, AIDS, anthrax, pneumonia (bacterial or viral), cellulitis, human parainfluenza, the common cold, Legionnaires' disease (Legionellosis), cholera, Creutzfeldt- Jakob disease (CJD), variant Creutzfeldt- Jakob disease (vCJD), fatal familial insomnia (FFI), Gerstmann-Straussler-Scheinker (GSS) syndrome, Chlamydia, chicken pox, ebola hemorrhagic fever, Dengue fever, giardiasis, Lyme disease, malaria, measles, mumps, rubella, pertussis, gonorrhea, staphyloc
  • Viruses include, but are not limited to, DNA or RNA animal viruses.
  • RNA viruses include, but are not limited to, virus families such as Picornaviridae (e.g., polioviruses), Reoviridae (e.g., rotaviruses), Togaviridae (e.g., encephalitis viruses, yellow fever virus, rubella virus), Orthomyxoviridae (e.g., influenza viruses), Paramyxoviridae (e.g., respiratory syncytial virus, measles virus, mumps virus, parainfluenza virus), Rhabdoviridae (e.g., rabies virus), Coronaviridae, Bunyaviridae, Flaviviridae, Filoviridae, Arenaviridae, Bunyaviridae and Retroviridae (e.g., human T cell lymphotropic viruses (HTLV), human immunodeficiency viruses (HIV)).
  • Picornaviridae
  • DNA viruses include, but are not limited to, virus families such as Papovaviridae (e.g., papilloma viruses), Adenoviridae (e.g., adenovirus), Herpesviridae (e.g., herpes simplex viruses), and Poxviridae (e.g., variola viruses).
  • Papovaviridae e.g., papilloma viruses
  • Adenoviridae e.g., adenovirus
  • Herpesviridae e.g., herpes simplex viruses
  • Poxviridae e.g., variola viruses
  • Bacteria include, but are not limited to, gram positive bacteria, gram negative bacteria, acid-fast bacteria and the like.
  • Gram positive bacteria include, but are not limited to, Actinomedurae, Actinomyces israelii, Bacillus anthracis, Bacillus cereus, Clostridium botulinum, Clostridium difficile, Clostridium perfringens,
  • Gram negative bacteria include, but are not limited to, Afipia felis, Bacteriodes, Bartonella baciUiformis, Bortadella pertussis, Borrelia burgdorferi, Borrelia recurrentis, Brucella, Calymmatobacterium granulomatis, Campylobacter, Escherichia coli, Francisella tularensis, Gardnerella vaginalis, Haemophilius aegyptius, Haemophilius ducreyi, Haemophilius influenziae, Heliobacter pylori, Legionella pneumophila, Leptospira interrogans, Neisseria meningitidia, Porphyromonas gingivalis, Providencia sturti, Pseudomonas aeruginosa, Salmonella enteridis, Salmonella typhi, Serratia
  • acid-fast bacteria include, but are not limited to, Myobacterium avium, Myobacterium leprae, Myobacterium tuberculosis and the like.
  • pediatric diseases, disorders, and conditions include, but are not limited to, autism, Kawasaki's disease, congenital deafness, pediatric cancers, Type I diabetes, congenital heart defects, tetralogy of Fallot, Duchenne Muscular Dystrophy, osteogenesis importfect, Krabe disease, Pompe disease, Gaucher disease, Fabry disease, Wolff-Parkinson- White syndrome,
  • pediatric conditions also include genetic attributes of the developing fetus.
  • pediatric conditions include, but are not limited to, intelligence, eye color, hair color, and muscle type.
  • "treating" a disease or condition refers to taking steps to obtain beneficial or desired results, including clinical results.
  • Beneficial or desired clinical results include, but are not limited to, alleviation or amelioration of one or more symptoms associated with diseases or conditions.
  • a compound or agent can also appropriately be introduced by rechargeable or biodegradable polymeric devices or other devices, e.g., patches and pumps, or formulations, which provide for the extended, slow, or controlled release of the compound or agent.
  • Administering can also be performed, for example, once, a plurality of times, and/or over one or more extended periods.
  • the administration includes both direct administration, including self- administration, and indirect administration, including the act of prescribing a drug.
  • a physician who instructs a patient to self-administer a drug, or to have the drug administered by another and/or who provides a patient with a prescription for a drug is administering the drug to the patient.
  • a physician who instructs a patient to self-administer a drug, or to have the drug administered by another and/or who provides a patient with a prescription for a drug is administering the drug to the patient.
  • a compound or an agent is administered orally, e.g., to a subject by ingestion, or intravenously, e.g., to a subject by injection.
  • the orally administered compound or agent is in an extended release or slow release formulation, or administered using a device for such slow or extended release.
  • a cell-free bodily fluid in combination with phagocytic cells or a cell-free bodily fluid in combination with fetal cells
  • up-regulation or up-regulated can refer to an increase in expression levels (e.g., gene expression or protein expression), gene copy numbers, gene dosages, and other qualitative or quantitative detectable state of the markers.
  • downstream- regulation or down-regulated can refer to an increase in expression levels, gene copy numbers, gene dosages, and other qualitative or quantitative detectable state of the markers.
  • the cellular contents of the phagocytic or >2n phagocytic cells comprise various types of materials that they have engulfed, such as viable diseased cells, dead diseased cells, apoptotic diseased cells, circulating tumor cells, infectious agents, fetal cells, trophoblasts, or fragments thereof.
  • at least one or more markers of a disease or condition are present in the cellular contents of the phagocytic or >2n phagocytic cells.
  • methods of this invention also comprise at least one of the following steps before determination of various profiles: i) lysing the circulating vesicles; and ii) extracting the contents from the lysed circulating vesicles.
  • the contents of the circulating vesicles comprise various types of materials, such as proteins and nucleic acids.
  • at least one or more markers of a disease or condition are present in the contents of the circulating vesicles.
  • methods of this invention also comprise at least one of the following steps before determination of various profiles: i) lysing the circulating diseased cells; and ii) extracting the contents from the lysed circulating diseased cells.
  • at least one or more markers of a disease or condition are present in the contents of the circulating diseased cells.
  • methods of this invention further comprise comparing the identified difference of the disease or condition-specific markers to a repository of at least one markers known in the art. Such comparison can further confirm the presence of the disease or condition.
  • the repository of the known markers can be obtained by data mining.
  • data mining refers to a process of finding new data patterns, relations, or correlations derived from the known data of the databases and of extracting practicable information in the future.
  • a computer-based system can be trained on data to perform the data mining, e.g., to classify the input data and then subsequently used with new input data to make decisions based on the training data.
  • systems include, but are not limited, expert systems, fuzzy logic, non-linear regression analysis, multivariate analysis, decision tree classifiers, and Bayesian belief networks.
  • Exemplary bodily fluid samples can be whole blood, urine, stool, saliva, lymph fluid,
  • cerebrospinal fluid cerebrospinal fluid, synovial fluid, cystic fluid, ascites, pleural effusion, fluid obtained from a pregnant woman in the first trimester, fluid obtained from a pregnant woman in the second trimester, fluid obtained from a pregnant woman in the third trimester, maternal blood, amniotic fluid, chorionic villus sample, fluid from a preimplantation embryo, maternal urine, maternal saliva, placental sample, fetal blood, lavage and cervical vaginal fluid, interstitial fluid, buccal swab sample, sputum, bronchial lavage, Pap smear sample, or ocular fluid.
  • Exemplary bodily fluid samples can be whole blood, urine, stool, saliva, lymph fluid, cerebrospinal fluid, synovial fluid, cystic fluid, ascites, pleural effusion, fluid obtained from a pregnant woman in the first trimester, fluid obtained from a pregnant woman in the second trimester, fluid obtained from a pregnant woman in the third trimester, maternal blood, amniotic fluid, chorionic villus sample, fluid from a preimplantation embryo, maternal urine, maternal saliva, placental sample, fetal blood, lavage and cervical vaginal fluid, interstitial fluid, buccal swab sample, sputum, bronchial lavage, Pap smear sample, or ocular fluid.
  • the cell-free bodily fluids are obtained by separating cells from the bodily fluid sample by methods known in the art, such as extraction, centrifugation, and filtration.
  • a component for use in a combination sample may be obtained by removing cells from a bodily fluid.
  • a component for use in a combination sample may be obtained by destroying (e.g., lysing) cells in a bodily fluid. These embodiments may be taken in combination, for example, by removing some populations of cells and destroying other populations of cells.
  • a sample of whole blood may be used to create a combination sample, for example, by removing red blood cells, serum, and T cells, and using the remainder as the combination sample.
  • tissue or fluid samples including cells having a DNA content of 2n are obtained post separation (e.g., via centrifugation) of non- cellular fraction of fluids obtained by puncture of a vein or artery followed by the withdrawal of blood, tissue biopsies, bronchoalveolar lavage, nasal lavage, eye lavage, peritoneal cavity lavage, vaginal lavage, bladder lavage, rectal lavage, fine needle aspiration of spinal fluid, synovial fluid aspiration, and the like.
  • Cell free bodily fluids are obtained post separation (e.g., via centrifugation) of cellular fraction of fluids obtained by puncture of a vein or artery followed by the withdrawal of blood, tissue biopsies, bronchoalveolar lavage, nasal lavage, eye lavage, peritoneal cavity lavage, vaginal lavage, bladder lavage, rectal lavage, fine needle aspiration of spinal fluid, synovial fluid aspiration, and the like.
  • cell separation/isolation/purification methods are used to isolate populations of cells from bodily fluid sample, cells, or tissues of a subject.
  • a skilled worker can use any known cell
  • Exemplary techniques include, but are not limited to, using antibodies, flow cytometry, fluorescence activated cell sorting, filtration, gradient-based centrifugation, elution, microfluidics, magnetic separation technique, fluorescent-magnetic separation technique, nanostructure, quantum dots, high throughput microscope-based platform, or a combination thereof.
  • cell separation/isolation/purification methods are used to isolate populations of circulating diseased cells from bodily fluid sample, cells, or tissues of a subject. Circulating diseased cells and fetal cells may be rare or in low quantity in a bodily fluid. Therefore, enrichment techniques (e.g., magnetic enrichment) may be used to enrich circulating diseased cells and/or fetal cells before the isolation.
  • enrichment techniques e.g., magnetic enrichment
  • a skilled worker can use any known cell
  • separation/isolation/purification techniques to isolate circulating diseased cells and/or fetal cells from a bodily fluid.
  • Exemplary techniques include, but are not limited to, using antibodies, flow cytometry, fluorescence activated cell sorting, filtration, gradient-based centrifugation, elution, microfluidics, magnetic separation technique, fluorescent-magnetic separation technique, nanostructure, quantum dots, high throughput microscope-based platform, micro-fluidic technique, fiber-optic array- scanning technique, laser-scanning cytometry technique, multiphoton intravital flow cytometry, photoacoustic flowmetry, nanoparticles targeting cell surface antigens, staining circulating diseased cells with detectable secreted products, or a combination thereof.
  • Circulating diseased cells may have different physical properties compared to normal circulating cells, such as difference in size, density, charge, migratory properties, and some properties of specific cell types (e.g., melanocytic granules in circulating melanoma cells).
  • a skilled worker can use any known cell
  • buoyant density may be used to separate circulating diseased cells (e.g., circulating tumor cells) from normal blood cells through gradient centrifugation.
  • Filtration-based approaches may be used isolate circulating diseased cells (e.g., circulating tumor cells) based on their increased sizes compared to normal circulating cells.
  • Antibody-based isolation approaches may be used to capture circulating diseased cells, which express epithelia cell surface markers that are absent from normal circulating blood cells. For example,
  • circulating diseased cells may be collected by the RareCellectTM device (Genetic Technologies) or similar devices.
  • the cell surface target is a protein that has been engulfed by >2n phagocytic cells.
  • the cell surface target is expressed by cells on their plasma membranes.
  • analytes include nucleic acids, proteins, lipids, carbohydrates, metabolites, or any combinations of these.
  • markers include nucleic acids, proteins, lipids, carbohydrates, metabolites, or any combinations of these.
  • nucleic acid is intended to include DNA molecules (e.g., cDNA or genomic DNA), RNA molecules (e.g., mRNA), DNA-RNA hybrids, and analogs of the DNA or RNA generated using nucleotide analogs.
  • the nucleic acid molecule can be a nucleotide, oligonucleotide, double-stranded DNA, single-stranded DNA, multi- stranded DNA, complementary DNA, genomic DNA, non-coding DNA, messenger RNA (mRNAs), microRNA (miRNAs), small nucleolar RNA (snoRNAs), ribosomal RNA (rRNA), transfer RNA (tRNA), small interfering RNA (siRNA), heterogeneous nuclear RNAs (hnRNA), or small hairpin RNA (shRNA).
  • the nucleic acid is a transrenal nucleic acid.
  • a transrenal nucleic acid is an extracellular nucleic acid that is excreted in the urine, See, e.g., U.S. Patent Publication No.
  • amino acid includes organic compounds containing both a basic amino group and an acidic carboxyl group. Included within this term are natural amino acids (e.g., L-amino acids), modified and unusual amino acids (e.g., D-amino acids and ⁇ -amino acids), as well as amino acids which are known to occur biologically in free or combined form but usually do not occur in proteins.
  • natural amino acids e.g., L-amino acids
  • modified and unusual amino acids e.g., D-amino acids and ⁇ -amino acids
  • Natural protein occurring amino acids include alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine, tryptophan, proline, and valine.
  • Natural non-protein amino acids include arginosuccinic acid, citrulline, cysteine sulfuric acid, 3,4-dihydroxyphenylalanine, homocysteine, homoserine, ornithine, 3-monoiodotyrosine, 3,5-diiodotryosine, 3, 5, 5- triiodothyronine, and 3,3', 5,5'- tetraiodothyronine.
  • Modified or unusual amino acids include D-amino acids, hydroxy lysine, 4-hydroxyproline, N-Cbz-protected amino acids, 2,4-diaminobutyric acid, homoarginine, norleucine, N-methylaminobutyric acid, naphthylalanine, phenylglycine, . alpha.
  • peptide includes compounds that consist of two or more amino acids that are linked by means of a peptide bond. Peptides may have a molecular weight of less than 10,000 Daltons, less than 5,000 Daltons, or less than 2,500 Daltons.
  • peptide also includes compounds containing both peptide and non-peptide components, such as pseudopeptide or peptidomimetic residues or other non-amino acid components. Such compounds containing both peptide and non-peptide components may also be referred to as a "peptide analog.”
  • protein includes compounds that consist of amino acids arranged in a linear chain and joined together by peptide bonds between the carboxyl and amino groups of adjacent amino acid residues. Proteins used in methods of the invention include, but are not limited to, amino acids, peptides, antibodies, antibody fragments, cytokines, lipoproteins, or glycoproteins.
  • antibody includes polyclonal antibodies, monoclonal antibodies (including full length antibodies which have an
  • lipoprotein includes negatively charged compositions that comprise a core of hydrophobic cholesteryl esters and triglyceride surrounded by a surface layer of amphipathic phospholipids with which free cholesterol and apolipoproteins are associated. Lipoproteins may be characterized by their density (e.g. very- low-density lipoprotein (VLDL), low-density lipoprotein (LDL) and high density lipoprotein (HDL)), which is determined by their size, the relative amounts of lipid and protein. Lipoproteins may also be characterized by the presence or absence of particular modifications (e.g. oxidization, acetylation, or gly cation). [0084] As used herein, the term "glycoprotein” includes glycosides which have one or more oligo- or polysaccharides covalently attached to a peptide or protein.
  • carbohydrate includes, but is not limited to, compounds that contain oxygen, hydrogen and carbon atoms, typically (CH 2 0) n wherein n is an integer.
  • Exemplary carbohydrates include, but are not limited to, monosaccharides, disaccharides, polysaccharides, or oligosaccharides.
  • metabolite includes any molecule used in metabolism. Metabolites can be products, substrates, or intermediates in metabolic processes. Included within this term are primary metabolites, secondary metabolites, organic metabolites, or inorganic metabolites. Metabolites include, without limitation, amino acids, peptides, acylcarnitines, monosaccharides, lipids and phospholipids, prostaglandins, hydroxyeicosatetraenoic acids,
  • hydroxyoctadecadienoic acids steroids, bile acids, and glycolipids and phospholipids.
  • exemplary metabolites can be sphingolipids, glycosphingolipids, sphingosine, ceramide, sphingomyelin, sphingosylphosphorylcholin, dihydrosphingosine, phoshatidylcholine, phosphatidylinositol, phosphatidylserine, lysophoshatidylcholme, lysophosphatidylinositol, lysophosphatidylserine, plasmenylphoshatidylcholine, plasmanylphoshatidylcholine, proteinogenic amino acids, Alanine, Aspartic acid, Glutamic acid, Phenylalanine, Glycine, Histidine, Leucine, Isoleucine, Lysine, Methionine, Proline, Argin
  • a sample may comprise one or more stabilizers for a cell or an analyte such as DNA, RNA, protein, and/or lipid.
  • a sample may comprise a DNA stabilizer, an RNA stabilizer, and/or a protein stabilizer.
  • Stabilizers are well known in the art and include, for example, DNAse inhibitors, RNAse inhibitors, and protease inhibitors or equivalents thereof.
  • Quantitative comparisons can include statistical analyses such as t-test, ANOVA, Krustal-Wallis, Wilcoxon, Mann- Whitney, and odds ratio. Quantitative differences can include differences in the levels of markers between profiles or differences in the numbers of markers present between profiles, and combinations thereof. Examples of levels of the markers can be, without limitation, gene expression levels, nucleic acid levels, protein levels, lipid levels, and the like.
  • Qualitative differences can include, but are not limited to, activation and inactivation, protein degradation, nucleic acid degradation, and covalent modifications.
  • the profile is a nucleic acid profile, a protein profile, a lipid profile, a carbohydrate profile, a metabolite profile, or a combination thereof.
  • the profile can be qualitatively or quantitatively determined.
  • a nucleic acid profile can be, without limitation, a genotypic profile, a single nucleotide polymorphism profile, a gene mutation profile, a gene copy number profile, a DNA methylation profile, a DNA acetylation profile, a chromosome dosage profile, a gene expression profile, or a combination thereof.
  • the nucleic acid profile can be determined by any methods known in the art to detect genotypes, single nucleotide polymorphisms, gene mutations, gene copy numbers, DNA methylation states, DNA acetylation states, chromosome dosages.
  • Exemplary methods include, but are not limited to, polymerase chain reaction (PCR) analysis, sequencing analysis, electrophoretic analysis, restriction fragment length polymorphism (RFLP) analysis, Northern blot analysis, quantitative PCR, reverse- transcriptase-PCR analysis (RT-PCR), allele-specific oligonucleotide hybridization analysis, comparative genomic hybridization, heteroduplex mobility assay (HMA), single strand conformational polymorphism (SSCP), denaturing gradient gel electrophisis (DGGE), RNAase mismatch analysis, mass spectrometry, tandem mass spectrometry, matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, electrospray ionization (ESI) mass spectrometry, surface- enhanced laser deorption/ionization-time of flight (SELDI-TOF) mass spectrometry, quadrupole-time of flight (Q-TOF) mass spectrometry, atmospheric pressure photoionization mass spectrometry (
  • sequencing is used in a broad sense and refers to any technique known in the art that allows the order of at least some consecutive nucleotides in at least part of a nucleic acid to be identified, including without limitation at least part of an extension product or a vector insert.
  • exemplary sequencing techniques include targeted sequencing, single molecule real-time sequencing, electron microscopy-based sequencing, transistor-mediated sequencing, direct sequencing, random shotgun sequencing, Sanger dideoxy termination sequencing, exon sequencing, whole-genome sequencing, sequencing by
  • sequencing comprises an detecting the sequencing product using an instrument, for example but not limited to an ABI PRISM® 377 DNA Sequencer, an ABI PRISM® 310, 3100, 3100-Avant, 3730, or 3730x1 Genetic Analyzer, an ABI PRISM® 3700 DNA Analyzer, or an Applied Biosystems SOLiDTM System (all from Applied Biosystems), a Genome Sequencer 20 System (Roche Applied Science), or a mass spectrometer.
  • sequencing comprises emulsion PCR.
  • sequencing comprises a high throughput sequencing technique, for example but not limited to, massively parallel signature sequencing (MPSS).
  • MPSS massively parallel signature sequencing
  • a protein profile can be a protein expression profile, a protein activation profile, or a combination thereof.
  • a protein activation profile can comprise determining a phosphorylation state, an ubiquitination state, a myristoylation state, or a conformational state of the protein.
  • a protein profile can be determined by an immunohistochemistry assay, an enzyme-linked immunosorbent assay (ELISA), in situ hybridization, chromatography, liquid chromatography, size exclusion chromatography, high performance liquid chromatography (HPLC), gas chromatography, mass
  • spectrometry tandem mass spectrometry, matrix assisted laser desorption/ionization- time of flight (MALDI-TOF) mass spectrometry, electrospray ionization (ESI) mass spectrometry, surface-enhanced laser deorption/ionization-time of flight (SELDI- TOF) mass spectrometry, quadrupole-time of flight (Q-TOF) mass spectrometry, atmospheric pressure photoionization mass spectrometry (APPI-MS), Fourier transform mass spectrometry (FTMS), matrix-assisted laser desorption/ionization- Fourier transform-ion cyclotron resonance (MALDI-FT-ICR) mass spectrometry, secondary ion mass spectrometry (SIMS), radioimmunoassays, microfluidic chip- based assay, detection of fluorescence, detection of chemiluminescence, or a combination thereof.
  • MALDI-TOF matrix assisted laser desorption/ionization- time of
  • One exemplary method of lipid analysis is to extract lipids from a biological sample (e.g.
  • fatty acid methyl esters e.g., using 14% BF3-methanol reagent
  • quantify the fatty acid methyl esters e.g., by HPLC, TLC, by gas chromatography-mass spectroscopy using commercially available gas chromatographs, mass spectrometers, and/or combination gas chromatograph/mass spectrometers.
  • Fatty acid mass is determined by comparing areas of various analyzed fatty acids to that of a fixed concentration of internal standard.
  • a carbohydrate profile can be determined by chromatography, liquid chromatography, size exclusion
  • MALDI-TOF matrix assisted laser desorption/ionization-time of flight
  • ESI electrospray ionization
  • SELDI-TOF surface-enhanced laser deorption/ionization-time of flight
  • Q-TOF quadrupole-time of flight
  • APPI-MS atmospheric pressure photoionization mass spectrometry
  • FTMS Fourier transform mass spectrometry
  • MALDI-FT-ICR desorption/ionization-Fourier transform-ion cyclotron resonance
  • SIMS secondary ion mass spectrometry
  • radioimmunoassays microfluidic chip-based assay, detection of fluorescence, detection of
  • a metabolite profile can be determind by chromatography, liquid chromatography, size exclusion chromatography, high performance liquid chromatography (HPLC), gas chromatography, mass
  • spectrometry tandem mass spectrometry, matrix assisted laser desorption/ionization- time of flight (MALDI-TOF) mass spectrometry, electrospray ionization (ESI) mass spectrometry, surface-enhanced laser deorption/ionization-time of flight (SELDI- TOF) mass spectrometry, quadrupole-time of flight (Q-TOF) mass spectrometry, atmospheric pressure photoionization mass spectrometry (APPI-MS), Fourier transform mass spectrometry (FTMS), matrix-assisted laser desorption/ionization- Fourier transform-ion cyclotron resonance (MALDI-FT-ICR) mass spectrometry, secondary ion mass spectrometry (SIMS), radioimmunoassays, microfluidic chip- based assay, detection of fluorescence, detection of chemiluminescence, or a combination thereof.
  • MALDI-TOF matrix assisted laser desorption/ionization- time of
  • the "difference" between different profiles detected by the methods of this invention can refer to different gene copy numbers, different DNA, RNA, protein, lipid, or carbohydrate expression levels, different DNA methylation states, different DNA acetylation states, and different protein modification states.
  • the difference can be a difference greater than 1 fold.
  • the difference is a 1.05-fold, 1.1 -fold, 1.2-fold, 1.3-fold, 1.4-fold, 1.5-fold, 2-fold, 2.5- fold, 3-fold, 4-fold, 5-fold, 6-fold, 7-fold, 8-fold, 9-fold, or 10-fold difference.
  • the difference is any fold difference between 1-10, 2-10, 5-10, 10-20, or 10-100 fold.
  • Down- regulated genes have X ⁇ 0, while up-regulated genes have X > 0. See, e.g., Efron, J Am Stat Assoc 104: 1015-1028 (2009).
  • a general principle of assays to detect markers involves preparing a sample or reaction mixture that may contain the marker (e.g., one or more of DNA, RNA, protein, polypeptide, carbohydrate, lipid, metabolite, and the like) and a probe under appropriate conditions and for a time sufficient to allow the marker and probe to interact and bind, thus forming a complex that can be removed and/or detected in the reaction mixture.
  • the marker e.g., one or more of DNA, RNA, protein, polypeptide, carbohydrate, lipid, metabolite, and the like
  • a probe under appropriate conditions and for a time sufficient to allow the marker and probe to interact and bind, thus forming a complex that can be removed and/or detected in the reaction mixture.
  • These assays can be conducted in a variety of ways.
  • one method to conduct such an assay would involve anchoring the marker or probe onto a solid phase support, also referred to as a substrate, and detecting target marker/probe complexes anchored on
  • a sample from a subject which is to be assayed for presence and/or concentration of marker, can be anchored onto a carrier or solid phase support.
  • the reverse situation is possible, in which the probe can be anchored to a solid phase and a sample from a subject can be allowed to react as an unanchored component of the assay.
  • biotinylated assay components can be prepared from biotin-NHS(N-hydroxy-succinimide) using techniques known in the art (e.g., biotinylation kit, Pierce Chemicals, Rockford, IL), and immobilized in the wells of streptavidin-coated 96 well plates (Pierce Chemical).
  • biotinylation kit Pierce Chemicals, Rockford, IL
  • streptavidin-coated 96 well plates Piereptavidin-coated 96 well plates
  • the surfaces with immobilized assay components can be prepared in advance and stored.
  • suitable carriers or solid phase supports for such assays include any material capable of binding the class of molecule to which the marker or probe belongs.
  • Well known supports or carriers include, but are not limited to, glass, polystyrene, nylon, polypropylene, nylon, polyethylene, dextran, amylases, natural and modified celluloses, polyacrylamides, gabbros, and magnetite.
  • the non- immobilized component is added to the solid phase upon which the second
  • marker/probe complexes anchored to the solid phase can be accomplished in a number of methods outlined herein.
  • the probe when it is the unanchored assay component, can be labeled for the purpose of detection and readout of the assay, either directly or indirectly, with detectable labels discussed herein and which are well-known to one skilled in the art.
  • marker/probe complex formation without further manipulation or labeling of either component (marker or probe), for example by utilizing the technique of fluorescence energy transfer (see, for example, U.S. Patent Nos. 5,631,169 and 4,868,103).
  • a fluorophore label on the first, 'donor' molecule is selected such that, upon excitation with incident light of appropriate wavelength, its emitted fluorescent energy will be absorbed by a fluorescent label on a second 'acceptor' molecule, which in turn is able to fluoresce due to the absorbed energy.
  • the 'donor' protein molecule may simply utilize the natural fluorescent energy of tryptophan residues.
  • Labels are chosen that emit different wavelengths of light, such that the 'acceptor' molecule label may be differentiated from that of the 'donor'. Since the efficiency of energy transfer between the labels is related to the distance separating the molecules, spatial relationships between the molecules can be assessed. In a situation in which binding occurs between the molecules, the fluorescent emission of the 'acceptor' molecule label in the assay should be maximal.
  • An FET binding event can be conveniently measured through standard fluorometric detection means well known in the art (e.g., using a
  • determination of the ability of a probe to recognize a marker can be accomplished without labeling either assay component (probe or marker) by utilizing a technology such as real-time Biomolecular Interaction Analysis (BIA) (see, e.g., Sjolander, S. and Urbaniczky, C, 1991, Anal. Chem. 63:2338 2345 and Szabo et al, 1995, Curr. Opin. Struct. Biol. 5:699 705).
  • BIOA Biomolecular Interaction Analysis
  • surface plasmon resonance is a technology for studying biospecific interactions in real time, without labeling any of the interactants (e.g., BIAcore).
  • analogous diagnostic and prognostic assays can be conducted with marker and probe as solutes in a liquid phase.
  • the complexed marker and probe are separated from uncomplexed components by any of a number of standard techniques, including but not limited to: differential centrifugation, chromatography, electrophoresis and immunoprecipitation.
  • marker/probe complexes may be separated from uncomplexed assay components through a series of centrifugal steps, due to the different sedimentation equilibria of complexes based on their different sizes and densities (see, for example, Rivas and Minton (1993) Trends Biochem. Sci. 18:284).
  • Standard chromatographic techniques may also be utilized to separate complexed molecules from uncomplexed ones. For example, gel filtration chromatography separates molecules based on size, and through the utilization of an appropriate gel filtration resin in a column format, for example, the relatively larger complex may be separated from the relatively smaller uncomplexed components.
  • the relatively different charge properties of the marker/probe complex as compared to the uncomplexed components may be exploited to differentiate the complex from uncomplexed components, for example through the utilization of ion-exchange chromatography resins.
  • ion-exchange chromatography resins Such resins and chromatographic techniques are well known to one skilled in the art (see, e.g., Heegaard (1998) J. Mol. Recognit. l l :141; Hage and Tweed (1997) J. Chromatogr. B. Biomed. Sci. Appl. 12:499).
  • electrophoresis may also be employed to separate complexed assay components from unbound components (see, e.g., Ausubel et al, ed., Current Protocols in Molecular Biology, John Wiley & Sons, New York, 1987 1999).
  • protein or nucleic acid complexes are separated based on size or charge, for example.
  • non-denaturing gel matrix materials and conditions in the absence of reducing agent are typically preferred. Appropriate conditions to the particular assay and components thereof will be well known to one skilled in the art.
  • the level of mRNA corresponding to the marker can be determined either by in situ and/or by in vitro formats in a biological sample using methods known in the art. Many expression detection methods use isolated RNA. For in vitro methods, any RNA isolation technique that does not select against the isolation of mRNA can be utilized for the purification of RNA from blood cells (see, e.g., Ausubel et al, ed., Current Protocols in Molecular Biology, John Wiley & Sons, New York 1987 1999). Additionally, large numbers of cells and/or samples can readily be processed using techniques well known to those of skill in the art, such as, for example, the single-step RNA isolation process of Chomczynski (1989, U.S. Patent No. 4,843,155).
  • Isolated mRNA can be used in hybridization or amplification assays that include, but are not limited to, Southern or Northern analyses, polymerase chain reaction analyses and probe arrays.
  • a diagnostic method for the detection of mRNA levels involves contacting the isolated mRNA with a nucleic acid molecule (probe) that can hybridize to the mRNA encoded by the gene being detected.
  • the nucleic acid probe can be, for example, a full-length cDNA, or a portion thereof, such as an oligonucleotide of at least 7, 15, 30, 50, 100, 250 or 500 nucleotides in length and sufficient to specifically hybridize under stringent conditions to an mRNA or genomic DNA encoding a marker of the present invention.
  • Other suitable probes for use in the diagnostic assays of the invention are described herein. Hybridization of an mRNA with the probe indicates that the marker in question is being expressed.
  • the mRNA is immobilized on a solid surface and contacted with a probe, for example by running the isolated mRNA on an agarose gel and transferring the mRNA from the gel to a membrane, such as nitrocellulose.
  • the probe(s) are immobilized on a solid surface and the mRNA is contacted with the probe(s), for example, in a gene chip array.
  • a skilled artisan can readily adapt known mRNA detection methods for use in detecting the level of mRNA encoded by the markers of the present invention.
  • An alternative method for determining the level of mRNA corresponding to a marker of the present invention in a sample involves the process of nucleic acid amplification, e.g., by RT-PCR (the experimental embodiment set forth in U.S.
  • Patent Nos. 4,683,195 and 4,683,202 COLD-PCR (Li et al. (2008) Nat. Med. 14:579), ligase chain reaction (Barany, 1991, Proc. Natl. Acad. Sci. USA, 88: 189), self sustained sequence replication (Guatelli et al, 1990, Proc. Natl. Acad. Sci.
  • mR A does not need to be isolated from the sample ⁇ e.g., a bodily fluid ⁇ e.g., blood cells)) prior to detection.
  • a cell or tissue sample is prepared/processed using known histological methods. The sample is then immobilized on a support, typically a glass slide, and then contacted with a probe that can hybridize to mRNA that encodes the marker.
  • determinations may be based on the normalized expression level of the marker.
  • a protein or polypeptide corresponding to a marker is detected.
  • an agent for detecting a protein or polypeptide can be an antibody capable of binding to the polypeptide, such as an antibody with a detectable label.
  • labeled with regard to a probe or antibody, is intended to encompass direct labeling of the probe or antibody by coupling (i.e., physically linking) a detectable substance to the probe or antibody, as well as indirect labeling of the probe or antibody by reactivity with another reagent that is directly labeled.
  • Examples of indirect labeling include detection of a primary antibody using a fluorescently labeled secondary antibody and end-labeling of a DNA probe with biotin such that it can be detected with fluorescently labeled streptavidin.
  • Antibodies can be polyclonal or monoclonal. An intact antibody, or a fragment thereof ⁇ e.g., Fab or F(ab')2) can be used. In one format, antibodies, or antibody fragments, can be used in methods such as Western blots or immunofluorescence techniques to detect the expressed proteins. In such uses, it is generally preferable to immobilize either the antibody or proteins on a solid support.
  • Suitable solid phase supports or carriers include any support capable of binding an antigen or an antibody.
  • Well known supports or carriers include glass, polystyrene, polypropylene, polyethylene, dextran, nylon, amylases, natural and modified celluloses,
  • a variety of formats can be employed to determine whether a sample contains a protein that binds to a given antibody.
  • formats include, but are not limited to, competitive and non-competitive immunoassay, enzyme immunoassay (EIA), radioimmunoassay (RIA), antigen capture assays, two-antibody sandwich assays, Western blot analysis, enzyme linked immunoabsorbant assay (ELISA), a planar array, a colorimetric assay, a chemiluminescent assay, a fluorescent assay, and the like.
  • EIA enzyme immunoassay
  • RIA radioimmunoassay
  • ELISA enzyme linked immunoabsorbant assay
  • a planar array a colorimetric assay, a chemiluminescent assay, a fluorescent assay, and the like.
  • Immunoassays including radioimmmunoassays and enzyme- linked immunoassays, are useful in the methods of the present invention.
  • a skilled artisan can readily adapt known protein/antibody detection methods for use in determining whether cells (e.g., bodily fluid cells such as blood cells) express a marker of the present invention.
  • cells e.g., bodily fluid cells such as blood cells
  • One skilled in the art will know many other suitable carriers for binding antibody or antigen, and will be able to adapt such support for use with the present invention.
  • protein isolated from cells e.g., bodily fluid cells such as blood cells
  • the support can then be washed with suitable buffers followed by treatment with the detectably labeled antibody.
  • the solid phase support can then be washed with the buffer a second time to remove unbound antibody.
  • the amount of bound label on the solid support can then be detected by conventional means.
  • an agent for detecting marker mRNA or genomic DNA is a labeled nucleic acid probe capable of hybridizing to marker mRNA or genomic DNA.
  • the nucleic acid probe can be, for example, a full-length marker nucleic acid or a portion thereof.
  • Other suitable probes for use in the diagnostic assays of the invention are described herein.
  • the assays are performed with a sample comprising a cell-free bodily fluid and a phagocytic cell, or with a sample comprising a cell-free bodily fluid and a fetal cell.
  • a compound capable of ameliorating or treating a disease or condition can include, without limitations, any substance that can improve symptoms or prognosis, prevent progression of the disease or condition, promote regression of the disease or condition, or eliminate the disease or condition.
  • the methods of the invention can also be used to detect genetic alterations in a marker gene, thereby determining if a subject with the altered gene is at risk for developing a disease and/or disorder associated with cancer and/or an infectious agent, and/or one or more other disorders described herein characterized by misregulation in a marker protein activity or nucleic acid expression, such as cancer.
  • the methods include detecting, in a cell free bodily fluid sample from the subject, the presence or absence of a genetic alteration characterized by an alteration affecting the integrity of a gene encoding a marker peptide and/or a marker gene.
  • such genetic alterations can be detected by ascertaining the existence of at least one of: 1) a deletion of one or more nucleotides from one or more marker genes; 2) an addition of one or more nucleotides to one or more marker genes; 3) a substitution of one or more nucleotides of one or more marker genes, 4) a chromosomal rearrangement of one or more marker genes; 5) an alteration in the level of a messenger RNA transcript of one or more marker genes; 6) aberrant modification of one or more marker genes, such as of the methylation pattern of the genomic DNA; 7) the presence of a non-wild type splicing pattern of a messenger RNA transcript of one or more marker genes; 8) a non-wild type level of a one or more marker proteins; 9) allelic loss of one or more marker genes; and 10) inappropriate post-translational modification of one or more marker proteins.
  • This method can include the steps of collecting a sample of cell free bodily fluid from a subject, isolating nucleic acid (e.g., genomic, mRNA or both) from the sample, contacting the nucleic acid sample with one or more primers which specifically hybridize to a marker gene under conditions such that hybridization and amplification of the marker gene (if present) occurs, and detecting the presence or absence of an amplification product, or detecting the size of the amplification product and comparing the length to a control sample. It is anticipated that PCR and/or LCR may be desirable to use as a preliminary amplification step in conjunction with any of the techniques used for detecting mutations described herein.
  • nucleic acid e.g., genomic, mRNA or both
  • Alternative amplification methods include: self sustained sequence replication (Guatelli et al, (1990) Proc. Natl. Acad. Sci. USA 87: 1874),
  • nucleic acid amplification method any other nucleic acid amplification method, followed by the detection of the amplified molecules using techniques well known to those of skill in the art. These detection schemes are especially useful for the detection of nucleic acid molecules if such molecules are present in very low numbers.
  • mutations in one or more marker genes from a sample can be identified by alterations in restriction enzyme cleavage patterns.
  • sample and control DNA is isolated, optionally amplified, digested with one or more restriction endonucleases, and fragment length sizes are determined by gel electrophoresis and compared. Differences in fragment length sizes between sample and control DNA indicates mutations in the sample DNA.
  • sequence specific ribozymes see, for example, U.S. Pat. No. 5,498,531 can be used to score for the presence of specific mutations by development or loss of a ribozyme cleavage site.
  • genetic mutations in one or more of the markers described herein can be identified by hybridizing a sample and control nucleic acids, e.g., DNA or RNA, to high density arrays containing hundreds or thousands of oligonucleotides probes (Cronin et al. (1996) Human Mutation 7: 244; Kozal et al. (1996) Nature Medicine 2:753).
  • a sample and control nucleic acids e.g., DNA or RNA
  • high density arrays containing hundreds or thousands of oligonucleotides probes e.g., DNA or RNA
  • genetic mutations in a marker nucleic acid can be identified in two dimensional arrays containing light-generated DNA probes as described in Cronin, M. T. et al. supra.
  • a first hybridization array of probes can be used to scan through long stretches of DNA in a sample and control to identify base changes between the sequences by making linear arrays of sequential overlapping probes. This step allows the identification of point mutations. This step is followed by a second hybridization array that allows the characterization of specific mutations by using smaller, specialized probe arrays complementary to all variants or mutations detected. Each mutation array is composed of parallel probe sets, one complementary to the wild-type gene and the other complementary to the mutant gene. [0127] In yet another embodiment, any of a variety of sequencing reactions known in the art can be used to directly sequence a marker gene and detect mutations by comparing the sequence of the sample marker gene with the corresponding wild-type (control) sequence.
  • sequencing reactions include those based on techniques developed by Maxam and Gilbert ((1977) Proc. Natl. Acad. Sci. USA 74:560) or Sanger ((1977) Proc. Natl. Acad. Sci. USA 74:5463).
  • any of a variety of automated sequencing procedures can be utilized when performing the diagnostic assays ((1995) Biotechniques 19:448), including sequencing by mass spectrometry (see, e.g., PCT International Publication No. WO 94/16101; Cohen et al. (1996) Adv. Chromatogr. 36: 127-162; and Griffm et al. (1993) Appl. Biochem. Biotechnol. 38: 147).
  • Other methods for detecting mutations in a marker gene include methods in which protection from cleavage agents is used to detect mismatched bases in
  • RNA/RNA or RNA/DNA heteroduplexes (Myers et al. (1985) Science 230: 1242).
  • the art technique of "mismatch cleavage" starts by providing heteroduplexes formed by hybridizing (labeled) RNA or DNA containing the wild-type marker sequence with potentially mutant RNA or DNA obtained from a tissue sample.
  • the double-stranded duplexes are treated with an agent which cleaves single-stranded regions of the duplex such as which will exist due to base pair mismatches between the control and sample strands.
  • RNA/DNA duplexes can be treated with RNase and DNA/DNA hybrids treated with S 1 nuclease to enzymatically digesting the mismatched regions.
  • either DNA/DNA or RNA/DNA duplexes can be treated with hydroxylamine or osmium tetroxide and with piperidine in order to digest mismatched regions. After digestion of the mismatched regions, the resulting material is then separated by size on denaturing polyacrylamide gels to determine the site of mutation. See, for example, Cotton et al. (1988) Proc. Natl. Acad. Sci. USA 85:4397; Saleeba et al. (1992) Methods Enzymol. 217:286.
  • the control DNA or RNA can be labeled for detection.
  • the mismatch cleavage reaction employs one or more proteins that recognize mismatched base pairs in double-stranded DNA (so called "DNA mismatch repair" enzymes) in defined systems for detecting and mapping point mutations in marker cDNAs obtained from samples of cells.
  • DNA mismatch repair enzymes
  • the mutY enzyme of E. coli cleaves A at G/A mismatches and the thymidine DNA glycosylase from HeLa cells cleaves T at G/T mismatches (Hsu et al. (1994) Carcinogenesis 15: 1657).
  • a probe based on a marker sequence e.g., a wild-type marker sequence
  • a marker sequence e.g., a wild-type marker sequence
  • the duplex is treated with a DNA mismatch repair enzyme, and the cleavage products, if any, can be detected from electrophoresis protocols or the like. See, for example, U.S. Patent No. 5,459,039.
  • alterations in electrophoretic mobility will be used to identify mutations in marker genes.
  • SSCP single strand conformation polymorphism
  • Single-stranded DNA fragments of sample and control marker nucleic acids will be denatured and allowed to renature.
  • the secondary structure of single-stranded nucleic acids varies according to sequence, the resulting alteration in electrophoretic mobility enables the detection of even a single base change.
  • the DNA fragments may be labeled or detected with labeled probes.
  • the sensitivity of the assay may be enhanced by using RNA (rather than DNA), in which the secondary structure is more sensitive to a change in sequence.
  • the subject method utilizes heteroduplex analysis to separate double stranded heteroduplex molecules on the basis of changes in electrophoretic mobility (Keen et al. (1991) Trends Genet. 7:5).
  • oligonucleotide primers may be prepared in which the known mutation is placed centrally and then hybridized to target DNA under conditions which permit hybridization only if a perfect match is found (Saiki et al. (1986) Nature 324: 163; Saiki et al. (1989) Proc. Natl. Acad. Sci. USA 86:6230).
  • Such allele specific oligonucleotides are hybridized to PCR amplified target DNA or a number of different mutations when the oligonucleotides are attached to the hybridizing membrane and hybridized with labeled target DNA.
  • amplification may also be performed using Taq ligase for amplification (Barany (1991) Proc. Natl. Acad. Sci. USA 88: 189). In such cases, ligation will occur only if there is a perfect match at the 3' end of the 5' sequence making it possible to detect the presence of a known mutation at a specific site by looking for the presence or absence of amplification.
  • the method comprises determining a first profile and/or a third profile of analytes from a sample comprising a cell-free bodily fluid and a population of non-phagocytes, or from a sample comprising a cell-free bodily fluid and a population of fetal cells.
  • this invention provides a method for identifying one or more markers of a disease or condition comprising: a) determining a first profile of analytes from a sample comprising a cell-free bodily fluid from a subject having said disease or condition and a population of phagocytic cells, or a population of >2n phagocytic cells, from a subject having said disease or condition, or a population of fetal cells; b) comparing the first profile to a second profile derived from a repository of analytes from a control subject not having said disease or condition; c) identifying a set of differences between the first and second profiles, wherein the set of differences is specific to the first profile relative to the second profile; and d) identifying one or more analytes specific to the set of differences, the identified analytes being markers of said disease or condition.
  • the method further comprises: e) obtaining a fifth profile of analytes from cells or tissues affected by said disease or condition in the subject having said disease or condition; obtaining a sixth profile of analytes from cells or tissues not affected by said disease or condition in the subject having said disease or condition; identifying a set of differences between the fifth and sixth profiles, wherein the set of differences is specific to the fifth profile relative to the sixth profile; and f) identifying at least one of the one or more markers of c) present in the set of differences identified in d).
  • this invention provides a method for identifying one or more markers that may be used in the treatment of a disease or condition.
  • a marker e.g., protein or gene
  • a marker identified by a method of the invention also may be used in any of the other methods of the invention, e.g., for monitoring the progression or regression of a disease or condition.
  • the one or more markers identified by the methods of this invention may have therapeutic potential. For example, if a marker is identified as being up-regulated (or down-regulated) in circulating diseased cells from a subject having a disease or condition , a compound or an agent that is capable of down-regulating (up-regulating) said marker may be useful in treating said disease or condition.
  • a marker e.g., protein or gene identified by a method of the invention may be used as a molecular target for a therapeutic agent.
  • a marker identified by a method of the invention also may be used in any of the other methods of the invention, e.g., for monitoring the progression or regression of a disease or condition.
  • the one or more markers identified by the methods of this invention may have therapeutic potential. For example, if a
  • gene/protein/lipid/carbohydrate expression profile a single nucleotide polymorphism profile, a gene mutation profile, a gene copy number profile, a DNA methylation profile, a DNA acetylation profile, a chromosome dosage profile, a gene expression profile, or a combination thereof may be useful in these embodiments.
  • An exemplary method for detecting the presence or absence of an analyte (e.g., DNA, RNA, protein, polypeptide, carbohydrate, lipid or the like) corresponding to a marker of the invention in a biological sample involves obtaining a bodily fluid sample (e.g., blood) from a test subject and contacting the bodily fluid sample with a compound or an agent capable of detecting one or more markers.
  • a bodily fluid sample e.g., blood
  • Detection methods described herein can be used to detect one or more markers in a biological sample in vitro as well as in vivo.
  • in vitro techniques for detection of mRNA include Northern hybridizations and in situ hybridizations.
  • In vitro techniques for detection of a polypeptide corresponding to a marker of the invention include enzyme linked immunosorbent assays (ELISAs), Western blots, immunoprecipitations and immunofluorescence.
  • In vitro techniques for detection of genomic DNA include Southern hybridizations.
  • in vivo techniques for detection of a polypeptide corresponding to a marker of the invention include introducing into a subject a labeled antibody directed against the polypeptide.
  • the antibody can be labeled with a radioactive marker whose presence and location in a subject can be detected by standard imaging techniques. Because each marker is also an analyte, any method described herein to detect the presence or absence of a marker can also be used to detect the presence or absence of an analyte.
  • the marker that is useful in the methods of the invention can include any mutation in any one of the above-identified markers. Mutation sites and sequences can be identified, for example, by databases or repositories of such information, e.g., The Human Gene Mutation Database (www.hgmd.cf.ac.uk), the Single Nucleotide Polymorphism Database (dbSNP, www.ncbi.nlm.nih.gov/projects/SNP), and the Online Mendelian Inheritance in Man (OMIM) website
  • the markers comprise at least one gene selected from the group consisting of AKT2, BAK1, EGFR, ERBB2, ETS2, FOS, JUN, MAP2K1 , MMP2, PDGFB, RB 1 , SERPINB2, SNCG, and SPP 1.
  • the one or more markers comprise at least one gene selected from the group consisting of AKT1, AKT2, BAK2, CDC25A, E2F1, EGFR, ERBB2, FOS, JUN, MAP2K1, MMP2, NFKB1, PDGFB, PIK3R1, PNN, RBI, SERPINB2, SERPINB5, SNCG, SPP1, TERT, TIMP3, and TP53.
  • the one or more markers comprise at least one gene selected from the group consisting of CASP8, CASP9,
  • the markers comprise at least one gene selected from the group consisting of ACP2, AK2, AKT3, ARL5B, ATP2B3, BGN, BRAF, BTG2, CAMKK2, CAPG, CAPN12, CPLX2, DENND5A, DNA2, FAM104A, FNIP1, GFRA4, GLUD1, GNAQ, GP1BB,
  • HNRPLL HNRPLL, HOXA2, HPS3, INPP4A, ITGAV, KLHL23, LANCL2, LYPD6,
  • the markers comprise at least one gene selected from the group consisting of B4GALT5, BOP1, CCL2, CCL3, CCL3L1, CCRL2, CD83, CLEC4G, CLIC4, CTSC, CTSO, CXCL10, FCGR3A, FPR3, HBA1, HBB, LRMP,
  • the markers comprise at least one gene selected from the group consisting of ACOT9, AMPD2, ARHGAP15, BATF2, C3AR1, C5orf41, CCL3, CCL3L1, CD63, CHST11, CHSY1, CLEC4G, CTSZ, CXorf21, CYTH4, CYTIP, DLEU2, DNAJA1, DOCK8, DTX3L, DUSP6, EPSTI1, ERF, F2RL1, FYB, GABRB2, GBP5, GLRX, GNB4, ICAM1, IFI35, IFIH1,
  • IFNAR2 IFNAR2, IL1R1, IRF1, ITGA5, LAP3, LAPTM5, LCP2, MAP1LC3B,
  • MAP1LC3B2 MICAL2, MT1DP, MT1JP, MT1M, MT2A, MYADML, NEK6, NINJ2, NNMT, NT5C3L, NUB1, PDE4B, PLOD1, PML, PRKCB, PSMB9, RCN3, RGS4, RNASE6, RTP4, SAMD9L, SEL1L, SERPING1, SETX, SIGLEC10, SKIL, SLC7A7, SNORA21, SP100, SP110, SP140, SSFA2, STAT2, STK17B, STK3,
  • the markers comprise at least one gene selected from the group consisting of ADAR, ADM, ALAS 1 , ANKRD22, ARHGAP27, B3GNT5, BCL10, C12orf35, C15orf29, C2orf59, CD177, CEACAM1, CPEB2, DDX58, F2RL1, GDPD3, GNAI3,
  • the markers comprise at least one biomarker selected from the group consisting of ACTN4, BC020163, CMIP, CNN2, EDNRB, GPM6B, KIT, MGC40222, NAMPT, PRAME, RPL18, RPL21, RPS15, TMEM80, TRIB2, TTC3, and VDAC1.
  • the markers are ACTN4, BC020163, CMIP, CNN2, EDNRB, GPM6B, KIT, MGC40222, NAMPT, PRAME, RPL18, RPL21, RPS15, TMEM80, TRIB2, TTC3, and VDAC1.
  • markers are useful in the diagnosis, prognosis, or monitoring of melanoma, or discriminating between different types of skin lesions, for example, melanoma and naevi (See, e.g., Wachsman et al, "Noninvasive genomic detection of melanoma,” Br J Dermatol. 2011
  • the marker that is useful in the methods of the invention for prenatal or pregnancy-related diseases or conditions include those disclosed in, for example, United States Patents 7,655,399, 7,651,838, 6,660,477, 6,172,198, 5,594,637, 5,514,598, 6,258,540, 6,664,056, 7,235,359, and 7,645,576, United States Patent Application Publications 20090162842, 20090155776,
  • 20070275402 20080153090, 20090170102, 20090061425, 20020045176,
  • the marker that is useful in the methods of the invention for neurological or neuropsychiatric diseases or conditions include those disclosed in, for example in United States Patents 7,723,117, 6,867,236, United States Patent Application Publications 20060115854, 20060115855, 20060166283,
  • the marker that is useful in the methods of the invention for cardiovascular diseases or conditions include those disclosed in, for example in United States Patents 7,670,769, 7,445,886, 7,432,107, 7,157,235, and 7,009,038, United States Patent Application Publications 20100167320,
  • the marker that is useful in the methods of the invention for autoimmune or immune -related diseases or conditions include those disclosed in, for example United States Patents 7,604,948, 7,670,764, 6,986,995, and 6,631,330, United States Patent Application Publication 20070141625, 20090263474, 20100075891, 20100104579, 20100105086, 20100131286, 20090176217,
  • kits that comprise marker detection agents that detect at least one or more of the markers identified by the methods of this invention.
  • This present invention also provides methods of treating or preventing a disease or condition in a subject comprising administering to said subject an agent that modulates the activity or expression or disrupts the function of at least one or more of the markers identified by the methods of this invention.
  • [0148] Separate blood sample into plasma and buffy coat including WBC sample. Coat plates to receive WBC sample with avidin.
  • biotinylated antibody to non-phagocytic blood cell (e.g., T cells) to the wells, incubate for 30 min at RT, wash wells.
  • non-phagocytic blood cell e.g., T cells
  • [0160] Separate plasma from whole blood.
  • [0161] 2. Use magnetic antibody-conjugated beads to isolate non-phagocytic (e.g., T cells) and phagocytic cells (e.g., neutrophils and/or macrophages and/or monocytes) from whole blood. Separate into phagocytic cells with DNA equal to 2n and DNA greater than 2n. Non-phagocytes and phagocytes having DNA equal to 2n are referred to as cells having DNA equal to 2n.
  • non-phagocytic e.g., T cells
  • phagocytic cells e.g., neutrophils and/or macrophages and/or monocytes
  • [0162] Separate blood sample into plasma and buffy coat including WBC sample. Stain WBC with fluorescent antibodies specific against a particular cell subpopulation (e.g., neutrophils, macrophages, monocytes, T cells and the like) and a DNA stain, (e.g., Hoechst 33342, Propidium iodide).
  • WBC fluorescent antibodies specific against a particular cell subpopulation
  • a DNA stain e.g., Hoechst 33342, Propidium iodide
  • cDNA or cRNA Prepare cDNA or cRNA and use to differentiate genetic profiles (e.g., a cancer gene array) between the combination sample and the control sample.
  • genetic profiles e.g., a cancer gene array
  • [0167] Isolate protein from the combination sample and from the control sample. Run Western blots using antibodies to known proteins overexpressed by human tumors (e.g., PSA and PSMA in prostate cancer; CEA in colon cancer; and CA125 in ovarian cancer), and compare the profiles obtained from the combination sample and the control sample. [0168] 5. Isolate lipids from the combination sample and from the control sample. Compare quantity and quality of lipids, for example using HPLC, between the combination sample and the control sample.
  • aneuploidies increase in frequency with maternal age and encompass potentially viable pregnancies with deviations from diploid number of chromosomes 13, 18 and 21, or abnormal numbers of X chromosomes in either male or female fetuses. Detection of these aneuploid conditions can occur by direct analysis of fetal cells collected by amniocentesis or chorionic villus sampling (CVS) and using traditional cytogenetic karyotyping or array comparative genome hybridization (CGH), quantitative PCR ( qPCR) or sequencing of fetal genomic DNA, but these approaches carry a risk of fetal or maternal morbidity and miscarriage as a consequence of the trauma of cell collection. Additionally, these invasive cell collection procedures are only effective when applied late in the first trimester of pregnancies, and earlier genotyping is preferred to enable earlier and better decisions on at risk pregnancies.
  • non-invasive procedures have been developed to detect fetal aneuploidies by analyzing the sequence composition of degraded genomic fetal DNA present at low levels in maternal blood, also known as circulating cell-free fetal DNA (ccff DNA).
  • ccff DNA circulating cell-free fetal DNA
  • these procedures rely on the exclusion of intact maternal genomic DNA from cellular components of blood to increase the observable signals that the procedures rely upon.
  • other methods use purely cellular fractions, but these methods may have high levels of maternal cell contamination (Bianchi et al., Proc Natl Acad Sci USA. 87(9)13279-3283 (1990); Bianchi et al., Am J Hum Genet.
  • an exemplary method was developed that harnesses the power of procedures reliant on cfff DNA and procedures reliant on cellular fractions alone.
  • the approach explicitly includes a cell-free bodily fluid and phagocytic blood cells expected to contain fetal genomic markers scavenged by phagocytosis of fetal cells in the placenta and other compartments.
  • paternal markers are DNA sequences that do not exist in the mother and are novel when comparing a strictly maternal DNA sample to a combination sample of maternal and fetal DNA.
  • paternal markers may be seen by DNA sequencing analyses in selected segments of the human genome which contain polymorphic sites. Polymorphic sites are often single nucleotide polymorphisms (SNPs), and distinguish alleles. Markers of this type are informative when the mother is homozygous for a SNP sequence and the father has a variant SNP passed to the fetus.
  • SNPs single nucleotide polymorphisms
  • the G allele of the SNP will be found at a proportion indicating the relative amount of fetal DNA in a sample.
  • the proportion of fetal material may be calculated by comparing the number of "A" bearing molecules (the maternal-derived material) to the number of "G” molecules (the fetal-derived material).
  • MAFs minor allele frequency
  • Plasma supernatant was centrifuged twice at high speed for 10 minutes to pellet and remove residual cells, and split into two samples per volunteer. 1% of resuspended monocytes, as defined by CD 14 expression were added to a plasma sample to create a combination sample. By defining monocytes by CD 14 expression, it is possible that the cell population also contains other phagocytes such as macrophages and neutrophils. A second plasma sample was used as a cell- free control. DNA was purified from the combination and cell-free control samples using the QIAamp Circulating Nucleic Acid Kit (Qiagen, 55114), and from the T-cell lymphocytes using the DNeasy Blood & Tissue Kit (Qiagen, 69506). DNA
  • Qubit®dsDNA BR Assay Kit (Life Technologies, Q32853). After DNA extraction, library preparation was performed using the primers listed in Table 1 , and massive parallel sequencing was performed on the Illumina MiSeqTM sequencer.
  • Library purification and generation prepare pooled, barcoded, and purified samples per the Illumina MiSeqTM manual for library generation. Dilute 10 nM samples to 2nM at 10 and add 10 ⁇ ⁇ 0.2 N NaOH. Incubate samples incubated at room temperature for 5 minutes and add 980 ⁇ ⁇ of HTI buffer to generate a 20 pM library. Further dilute the library to 12.5 pM in HTI buffer and load with a 1 % phiX spike.
  • the data shows 16 combination sample-specific MAFs that are informative, i.e., SNPs for which the mother was homozygous and the fetus was heterozygous ( Figure 1 , Table 2).
  • This value is very near the theoretical 10% (16/167) expectation (Tynan et al, J Mol Diagn. 13(4):382-9 (201 1)), confirming fetal content in the sample.
  • the presence of 16 informative MAFs falls above the level for 99.9% confidence established in Tynan et al., J Mol Diagn. 13 (4): 382-9 (2011).
  • the fetal DNA content in the combination sample was confirmed.
  • Figures 1 and 2 show potentially informative alleles from two assays plotted against the percent of reads of each allele in maternal and combination samples.
  • a rough estimate of informative MAFs can be seen by the boxed off sections at the top and bottom of the graph.
  • informative MAFs are seen where an allele in a combination sample shows major and minor frequencies. The significance of these differenced can be determined by a number of statistical analyses known in the art (for example, values that are five standard deviations from the mean value or other statistical measures).
  • Figure 2 in particular shows tight clustering of data around the 50% mark, demonstrating a low standard deviation.
  • Figure 5 shows the improvement in data between a combination sample (cell-free bodily fluid plus 1% monocytes) when compared to a cell-free bodily fluid or T-cell sample alone ( Figures 3 and 4, respectively).
  • Figures 6 and 7 are simplified to show only the informative markers, and demonstrate that the combination sample produces "cleaner" data than a cell-free bodily fluid sample without monocytes.
  • Figures 8 and 9 show exemplary informative markers. Both depict a maternal only sample wherein the reference allele reads are 100% and the alternative allele reads are 100%, and a combination sample that contains reads of the alterative allele (and, necessarily, fewer reads of the reference allele such that the values add to 100%).
  • the exemplary non-informative marker depicted in Figure 10 has reads of approximately 50% of reference and alternative alleles in both the maternal only and the combination sample.
  • reproducibility in combination samples is better than in corresponding cell-free bodily fluid samples (Table 3).
  • Table 3 the differences between Assay 1 and Assay 2 may be because a low-performing marker was removed in Assay 2 and one of the markers in Assay 2 also was rejected due to a threshold effect with the computational percentage (to include all base calls at a position rather than only the reference and alternative markers).
  • the DNA produced during the highly ordered apoptotic process may exist in a less degraded state than the DNA
  • DNA produced during non-apoptitic cell death is exposed to a variety of harmful subcellular components including DNA
  • circulating vesicles, and circulating diseased cells may contribute higher quality
  • the proof-of-principle here shows that diagnosis of, for example, fetal aneuploidy can be accomplished by selecting an appropriate panel of informative
  • MAF SNPs weighted to the chromosomes of interest e.g., chromosome 21
  • determination of fetal content by read depth computation e.g., fetal content by read depth computation
  • Maternal plasma previously characterized to contain 5% fetal fraction of circulating cell-free DNA was mixed with cells harvested from the same woman at calculated ratios, using the assumption that each cell contained 6 picograms of DNA. The cells were harvested by amniocentesis, which is highly enriched for fetal cells. The total DNA in the plasma was shown photometrically to be 200 nanograms.
  • Theoretical ratios of the fractions of fetal DNA content as compared to the total DNA content were thereby attained, with the assumption that the harvested cells were highly enriched for fetal content, at 5% (0 added cells), 10% (2000 added cells), 15% (4000 added cells), and 20% (6000 added cells).
  • DNA was subsequently extracted and purified from each sample, and the purified DNA mixtures from each individual were subjected to a 593 -primer PCR reaction. Purified DNA from all the maternal plasma and compound samples was treated identically, and all assays were performed in triplicate. The amplicons generated by this method were sequenced using a paired- end strategy at a target depth of approximately 10 million reads per sample.
  • a fetus would inherit a paternal allele that could be distinguished from the mother (e.g., the mother is homozygous AA, and the fetal genotype is heterozygous AB, so the B allele must have been contributed by the father) at >22% of the SNPs (130 SNPs in total) targeted in this reaction.
  • the allele frequencies at each site were determined by sequencing the generated amplicons and counting the number of sequencing reads associated with each allele. Using this technique, the allele frequencies determined from sequencing were used to determine the fetal fraction in a sample from a pregnant woman.
  • Sequencing reads were aligned to a reference genome, and the allele A ratios were calculated for each SNP site by dividing the allele A reads by the sum of the allele A and B reads.
  • the genomic samples were used to determine the genotype for both the maternal and fetal samples. Sites with allele A ratios between 10% and 90% were designated heterozygous. Sites with allele A ratios >90%> were designated homozygous for allele A, and sites with allele A ratios ⁇ 10% were designated homozygous for allele B. Of the 593 sites targeted in this assay, approximately 100 were deemed informative because the mother was homozygous and the fetus was heterozygous.
  • the allele A ratio of each useful site was then calculated for the mixed DNA samples as in the circulating cell-free fetal (ccff) samples.
  • the fetal fraction for each sample was calculated from the mean allele A frequency using the formula 2 x (100% - mean allele A ratio). For example, if the allele A frequency is 0.9, fetal DNA content is 20% in the combined sample.

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Abstract

L'invention concerne des méthodes d'utilisation d'un échantillon présentant de multiples composants analytiques dans le diagnostic, le pronostic, ou la surveillance de maladies ou d'états pathologiques. L'invention concerne également des méthodes d'identification de marqueurs de maladies ou d'états pathologiques.
PCT/US2014/070907 2013-12-17 2014-12-17 Méthodes de détection de maladies ou d'états pathologiques Ceased WO2015095359A1 (fr)

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Cited By (4)

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Publication number Priority date Publication date Assignee Title
WO2019108906A1 (fr) * 2017-11-30 2019-06-06 Baylor College Of Medicine Méthylation de l'adn génomique associée à une prédiction de maladie
CN113533170A (zh) * 2020-04-21 2021-10-22 贝克曼考尔特公司 病毒感染的血液学参数
US11685951B2 (en) 2017-07-18 2023-06-27 The Research Foundation For The State University Of New York Biomarkers for intracranial aneurysm
CN118995917A (zh) * 2024-09-30 2024-11-22 暨南大学 Ppif基因作为标志物在子宫内膜异位症诊断评估中的应用

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US20050181463A1 (en) * 2004-02-17 2005-08-18 Rao Galla C. Analysis of circulating tumor cells, fragments, and debris
WO2009092068A1 (fr) * 2008-01-18 2009-07-23 President And Fellows Of Harvard College Procédés de détection de signatures d'une maladie ou d'états dans des fluides corporels
WO2012012693A2 (fr) * 2010-07-23 2012-01-26 President And Fellows Of Harvard College Procédés de détection de signatures de maladies ou pathologies dans des liquides biologiques
US20120040846A1 (en) * 2010-07-23 2012-02-16 President And Fellows Of Harvard College Methods of Detecting Diseases or Conditions Using Phagocytic Cells
WO2013188846A1 (fr) * 2012-06-15 2013-12-19 Harry Stylli Procédés de détection de maladies ou d'états

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Publication number Priority date Publication date Assignee Title
US20050181463A1 (en) * 2004-02-17 2005-08-18 Rao Galla C. Analysis of circulating tumor cells, fragments, and debris
WO2009092068A1 (fr) * 2008-01-18 2009-07-23 President And Fellows Of Harvard College Procédés de détection de signatures d'une maladie ou d'états dans des fluides corporels
WO2012012693A2 (fr) * 2010-07-23 2012-01-26 President And Fellows Of Harvard College Procédés de détection de signatures de maladies ou pathologies dans des liquides biologiques
US20120040846A1 (en) * 2010-07-23 2012-02-16 President And Fellows Of Harvard College Methods of Detecting Diseases or Conditions Using Phagocytic Cells
WO2013188846A1 (fr) * 2012-06-15 2013-12-19 Harry Stylli Procédés de détection de maladies ou d'états

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11685951B2 (en) 2017-07-18 2023-06-27 The Research Foundation For The State University Of New York Biomarkers for intracranial aneurysm
WO2019108906A1 (fr) * 2017-11-30 2019-06-06 Baylor College Of Medicine Méthylation de l'adn génomique associée à une prédiction de maladie
CN113533170A (zh) * 2020-04-21 2021-10-22 贝克曼考尔特公司 病毒感染的血液学参数
CN118995917A (zh) * 2024-09-30 2024-11-22 暨南大学 Ppif基因作为标志物在子宫内膜异位症诊断评估中的应用

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