WO2015093634A1 - Use of extract of cichorium intybus for preventing, treating, or alleviating muscular damage - Google Patents

Abstract

The present invention relates to a pharmaceutical composition containing an extract of Cichorium intybus as an active ingredient for preventing or treating myoatrophy; a food composition containing an extract of Cichorium intybus for preventing or treating myoatrophy; and a use of an extract of Cichorium intybus for preventing, treating, or alleviating muscular damage. The pharmaceutical composition and food composition containing the extract of Cichorium intybus according to the present invention can be favorably used to prevent, alleviate, or treat myoatrophy by suppressing myocyte damage, necrosis, and apoptosis.

Description

Use for preventing, treating or improving muscle damage of chicory extract

The present invention is chicory ( Cicchorium intybus ) A pharmaceutical composition for preventing or treating muscle atrophy containing the extract as an active ingredient, a food composition for preventing or improving muscle atrophy containing chicory extract, and a use for preventing, treating or improving muscle damage of chicory extract.

Atrophy can be defined as the loss of muscle tissue that results from not using muscle or the disease of the muscle itself or damage to the nerves that control the muscle. In general, the most important cause of muscle atrophy is disuse atrophy caused by not using muscle. In other words, in the case of a socially declining activity, muscle tone itself decreases and gradually progresses to muscular atrophy. In addition, people living in places where there is no gravity will also have a drop in muscle strength due to a decrease in calcium and muscle strength.

In addition, muscle atrophy due to the disease of the muscle itself is myasthenia gravis, dystrophy: dystrophic dystrophy, myotonic dystrophy, duchenne type, Becker's type, belt type, facial scapula and inflammation of the muscle itself. Muscle atrophy due to damage to the nerves that dominate the muscles; spinal muscular amyotrophy: Berradnich-Hoffmann's type, Kugelberg-Belander's disease, amyotrophic lateral sclerosis (ALS) : Lou Gehrig's disease and spinobular muscular atrophy: Kennedy's disease.

The cause of muscular dystrophy has not yet been elucidated, and to date, the main causes of the development of muscular dystrophy have been suggested in terms of muscle cell damage caused by increased oxide quality, decreased muscle protein production-regeneration due to decreased stress protein expression, or activation of proteosomal ubiquitination. And muscle atrophy, and muscle atrophy is diagnosed through creatine kinase (CK), electromyography, muscle biopsy, molecular biological genetic testing, cytogenetic testing, and the like.

On the other hand, not only muscular dystrophy, muscle weakness, dysphagia, and dysphagia are the main treatment methods for the symptoms that can be caused by myasthenia gravis, stroke, senile dementia, Parkinson's disease, diabetes, amyotrophic lateral sclerosis, The only response is physiotherapy, combined with occupational therapy to prevent complications and malformations, dysfunction, respiratory therapy and additionally symptomatic and supportive therapy.

[Preceding technical literature]

[Patent Documents]

(Patent Document 1) 1. Korean Registered Patent Publication No. 10-0899377

(Patent Document 2) 2. Korean Laid-Open Patent Publication No. 10-2012-0047510

[Non-Patent Documents]

(Non-Patent Document 1) 1. Yoo Shin-Soo and 9 others, The Korean Society of Medicinal Crop Science Conference 2011 Spring Conference, Page 286-287

To date, treatment for muscle disease is an adjuvant therapy for symptoms, and it is hard to expect to suppress or cure the onset of muscle atrophy. Therefore, there is a need for the development of new materials that can treat muscular dystrophy.

Accordingly, the present invention seeks to discover new materials for the prevention, treatment or improvement of muscular dystrophy and to provide new uses thereof.

In order to achieve the above object, the present inventors have conducted research to find a substance that inhibits muscle damage, necrosis and apoptosis from natural products, and as a result, chicory extract suppresses myocyte damage, necrosis and apoptosis by oxidative stress. As a result, it was confirmed that muscle atrophy can be prevented, treated or improved.

Accordingly, the present invention provides a pharmaceutical composition and a food composition for preventing, treating or improving muscle atrophy comprising chicory extract as an active ingredient.

Chicory extract can be usefully used as an active ingredient for preventing, treating or improving muscle atrophy by inhibiting myocyte damage, necrosis and apoptosis.

1 is a graph showing the effect of chicory extract (root extract and stem extract) in the concentration-dependent prevention and recovery of muscle cells necrosis in muscle cells induced muscle cell necrosis with H ₂ O ₂ .

2 is a graph showing the effect of chicory extract (root extract and stem extract) in the concentration-dependent prevention and recovery of muscle cell necrosis in muscle cells induced muscle cell necrosis with H ₂ O ₂ , NAC as a positive control group N-acetyl cysteine.

Figure 3 is a graph showing the cytotoxicity when cultured in muscle cells after treatment with chicory extract (root extract and stem extract) 5, 10, 25 and 50 μg / ml.

4 is a graph showing the apoptosis inhibitory effect in muscle cells after 25 and 50 μg / ml of chicory root extract, NAC refers to N-acetyl cysteine as a positive control.

5 is a graph showing the results of measuring the effect of chicory root extract on H ₂ O ₂ -induced apoptosis of C2C12 muscle cells.

Figure 6 is a graph showing the results of measuring the ceramide level change when H ₂ O ₂ -treated C2C12 muscle cells treated with chicory root extract for each concentration.

Figure 7 is a graph showing the results of measuring the Hsp70 level change when treated with chicory root extract concentration to H ₂ O ₂ -treated C2C12 muscle cells.

The present invention provides a pharmaceutical composition and a food composition for preventing, treating or improving muscle atrophy comprising chicory extract as an active ingredient.

The present invention provides a pharmaceutical composition for preventing or treating muscular atrophy and a food composition for preventing or improving muscular atrophy comprising extracts of chicory flowers, leaves, branches, fruits, roots, stems, seeds or mixtures thereof as an active ingredient. .

EMBODIMENT OF THE INVENTION Hereinafter, the structure of this invention is demonstrated concretely.

Chicory, which is used as an active ingredient in the composition of the present invention, is a perennial herb belonging to the Asteraceae family, and is known to be effective in improving liver and restoring vision, and is known to be a beneficial bacterium among intestinal bacteria when chicory intake is increased. It is reported that the number of bacteria of Bifidobacteria and Lactobacillus increases significantly. It has also been reported that intake of chicory consumes almost no production of methane gas in the human intestine and doubles the production of organic acids such as lactic acid.

Ingestion of chicory results in a 50% or more reduction in cholesterol absorption in the intestine, which acts as a factor in lowering the total cholesterol content in the liver or blood by preventing chicory extracts from re-absorbing cholesterol and excreting it into feces. . In addition, as a result of examining the effect of chicory extract on the intake of glucose, the total glycated hemoglobin content used as an indicator of the average blood glucose level was significantly lower in the chicory intake. In other words, ingesting chicory can reduce the absorption of glucose can be confirmed to be effective in the prevention or treatment of diabetes.

Chicory has been used as a herb for a long time, and has been reported to greatly affect liver and digestive function recovery. Chicory has been used as an herbal remedy for clearing blood, relieving emotional excitement, and for many diseases. It is effective for loss of appetite, jaundice and cholelithiasis in the body, and leaves are used for inflammation and swelling of the skin in vitro, and it is also used for the treatment of gout and rheumatism by promoting the discharge of unnecessary moisture in the body as a diuretic. The roots and leaves of chicory are currently used as an appetite stimulant, bile stimulant for the secretion of bile, purifying agent to remove toxins and wastes in the body, digestive agent, hypoglycaemia and laxative to help defecation. In addition, chicory has been reported to be effective in improving blood circulation by facilitating the exchange of blood flow and fluid through veins and arteries. The roots of chicory are either used directly or dried, and are widely used medically. Root extracts have been tested experimentally for heart disease and abnormalities by making them have a weak pulse rate (heart rate).

Chicory is also used as a kind of Baja flower therapy to treat diseases with flowers or plants in response to various emotions and heart conditions such as strong possessiveness, self-pity and narcissism. Chicory stem extract is used for skin diseases such as warts, which also heal or eliminate it. Treatment guides for German herbs report that chicory is effective in improving anorexia and indigestion.

In addition, chicory has potential as a biomass resource that could be useful for the industry. Chicory contains many inulins, which can be easily converted to alcohol. In addition, the leaves are extracted with dyes, and the flowers are dried, powdered, mixed with other herbs, and added to fertilizers, which can be used to make compost in a short time.

However, no examples of using chicory for use in the prevention, treatment or amelioration of muscular dystrophy have been reported. Accordingly, the present invention is characterized by providing novel uses for the prevention, treatment or amelioration of chicory atrophy.

Chicory extract used as an active ingredient of the pharmaceutical composition or the food composition of the present invention may be prepared according to a conventional method for producing a plant extract.

In one embodiment, the chicory extract may be extracted from the flowers, leaves, branches, fruits, roots, stems, seeds or mixtures of chicory with a conventional solvent.

In one embodiment of the invention, the chicory extract may be a root extract of chicory.

The extraction solvent used for the preparation of the chicory extract may be, but is not limited to, a solvent selected from water, an organic solvent or a mixture thereof. The organic solvent may be a polar solvent such as alcohol having 1 to 5 carbon atoms, ethyl acetate, acetone, a nonpolar solvent such as ether, chloroform, benzene, hexane, dichloromethane, or a mixed solvent thereof. For example, the alcohol having 1 to 5 carbon atoms may be selected from methanol, ethanol, propanol, butanol, isopropanol and the like.

In the present invention, the chicory extract includes a fraction obtained by re-fractionation of the primary extract extracted using the extraction solvent using an extraction solvent having a different polarity. For example, the chicory extract may be a fraction obtained by extracting chicory with an alcohol having 1 to 5 carbon atoms and then re-dividing the chicory with a solvent having a different polarity such as ether, benzene, and hexane.

Two or more solvents may be used in the fractionation, and each solvent extract may be prepared using sequentially or mixed according to the polarity of the solvent, but is not limited thereto.

The prepared extract or the fraction obtained by performing the fractionation process can be filtered or concentrated or dried to remove the solvent, it can be carried out both filtration, concentration and drying. Specifically, the filtration may be performed using a filter paper or a reduced pressure filter, the concentration may be concentrated under reduced pressure using a reduced pressure concentrator, for example, a rotary evaporator, and the drying may be performed by, for example, a lyophilization method.

In one embodiment of the present invention, the pharmaceutical composition for preventing and treating muscle atrophy according to the present invention dominates muscle atrophy due to the loss of muscle tissue caused by not using the muscle, muscle atrophy due to the disease of the muscle itself, muscle It can be used for the prevention and treatment of muscular atrophy caused by nerve damage.

As can be seen in the following examples, the chicory extract can suppress myocyte damage, necrosis and apoptosis, and because it is a natural material has no side effects and very high safety, it can safely and effectively prevent and treat muscle atrophy.

The pharmaceutical composition according to the present invention may further include a carrier and a vehicle commonly used in the pharmaceutical field. Particularly glyceride mixtures of ion exchange resins, alumina, aluminum stearate, lecithin, serum proteins (e.g. human serum albumin), buffer substances (e.g. various phosphates, glycine, sorbic acid, potassium sorbate, saturated vegetable fatty acids ), Water, salts or electrolytes (e.g., protamine sulfate, disodium hydrogen phosphate, hydrogen carbonate, sodium chloride and zinc salts), colloidal silica, magnesium trisilicate, polyvinylpyrrolidone, cellulose based substrates, polyethylene glycols, sodium carboxy Methylcellulose, polyarylate, wax or wool, and the like, but are not limited thereto.

The pharmaceutical compositions may also be in the form of granules, powders, coated tablets, tablets, capsules, suppositories, syrups, juices, suspensions, emulsions, drops, injectables or sustained release formulations of the active compounds. Pharmaceutical compositions for the prevention and treatment of muscular atrophy can be administered in a variety of oral or parenteral formulations, when formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc. commonly used in the pharmaceutical field It can be prepared using.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient in the pharmaceutical composition of the present invention, for example, starch, calcium carbonate, It may be prepared by mixing sucrose or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. .

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used. Witsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like may be used as the base material of the suppository.

On the other hand, the dosage of the pharmaceutical composition according to the present invention, the type of disease, the severity of the disease, the type and amount of the active ingredient and other ingredients contained in the composition, the type of formulation and the age, weight, general state of health, sex and It can be adjusted according to various factors including the diet, the time of administration, the route of administration and the rate of secretion of the composition, the duration of treatment, and the drugs used simultaneously. For example, in adults, the chicory extract of the present invention may be administered at a dose of 0.01 to 500 mg / kg, once or several times daily. Specifically, 0.1 to 200 mg / kg, more specifically 0.1 to 100 mg / kg may be administered 1 to 3 times daily. The unit dosage form thus formulated may be administered several times at regular time intervals as needed.

The present invention also relates to a food composition for muscular atrophy comprising chicory extract as an active ingredient.

In one embodiment of the present invention, the food composition for muscle atrophy including chicory extract can be used as a health functional food that can be ingested daily to inhibit muscle aging or improve motor function.

In the present specification, 'health functional food' is a food prepared by adding chicory extract to food materials such as beverages, teas, spices, gums, confectionery, encapsulated, powdered, suspensions, etc. Means to bring the effect, unlike the general medicine has the advantage that there are no side effects that can occur when taking a long-term use of the drug as a raw material. The health functional food of the present invention thus obtained is very useful because it can be consumed on a daily basis.

In addition, in one embodiment of the present invention, the food composition for muscle atrophy including chicory extract can be used as a space food prepared by adding to various foods for inhibiting muscle aging due to space life or improving exercise function. In the present specification, 'space food' is made by adding chicory extract to various foods as a food prepared to supplement the problems caused by space life. The chicory extract may be added as it is or used in combination with other food or food ingredients, and the mixed amount of the chicory extract may be appropriately determined according to conventional methods depending on the purpose of use thereof.

The food composition according to the present invention includes all foods such as beverages, meat, chocolate, foods, confectionary, pizza, ramen, other noodles, gums, ice creams, alcoholic beverages, vitamin complexes and the like. Although it cannot be prescribed | regulated uniformly according to the kind of food, what is necessary is just to add in the range which does not impair the original taste of food, and it is the range of 0.01-50 weight% normally, specifically 0.1-20 weight% with respect to the target food. In the case of food in the form of granules, tablets or capsules, it is usually added in the range of 0.1 to 100% by weight, specifically 5 to 100% by weight.

DETAILED DESCRIPTION The present invention will become apparent with reference to the embodiments described below in detail of their advantages and features, and how to achieve them. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in various forms, and only the embodiments are intended to complete the disclosure of the present invention, and the general knowledge in the technical field to which the present invention belongs. It is provided to fully convey the scope of the invention to those skilled in the art, and is only defined by the scope of the claims of the invention.

EXAMPLE

Example 1: Preparation of Chicory Extract

Chicory was cultivated by the RDA's Good Agricultural Practice (GAP), and was harvested in 2009 in Chungcheongbuk-do Negong (GPS: E 128 ° 62 'N 36 ° 56'). The sample was extracted as follows. 50 g of chicory root sample and 50 g of chicory stem sample were added to 150 ml of 99% ethanol, respectively, and reacted for 48 hours at 150 rpm. The resulting mixture was concentrated under reduced pressure and lyophilized powder was used as a sample of this experiment.

Experimental Example Confirmation of Biological Activity

Experimental Example 1: Improvement of survival rate through antioxidant in muscle cell culture system

(1) Cell culture

Normal muscle cells (C ₂ C ₁₂ ) were cultured in DMEM medium containing 10% fetal bovine serum (FBS). In a 75 cm 2 plastic flask (Falcon Co., England), normal skeletal muscle cells were loaded with 10% FBS, 7.5% NaHCO ₃ 150 μg / ml, 58.4 μg / ml glutamine and 4.4 μl / antibiotic / antimycotics. The cells were incubated at 37 ° C. and 5% CO ₂ in DMEM medium containing ml. Cell lines were maintained by secondary culture once every 2-3 days.

(2) cell quantification

Remove the medium solution from the grown 75 cm 2 plastic flask, wash with CMF-PBS (calcium magnesium free-phosphate buffered saline, pH 7.2), remove the cells from the bottom of the flask by treatment with 0.25% trypsin / EDTA. It was neutralized with cell culture and centrifuged (1200 rpm, 5 min). Culture medium was added to the pellets of the remaining cells, and then aspirated repeatedly with a sterile pipette to make a single cell suspension, and measured using a hemocytometer on an optical microscope.

(3-1) Concentration Effects of Chicory Extract on Muscle Cell Necrosis Induced by H ₂ O ₂

In order to observe the chicory extract concentrations effect of (root extract and stem extract) for the cell death induced by H ₂ O _2, placed such that the first muscle cells 5 × 10 ⁴ cells / well in 96 well plates CO ₂ incubator Incubated at. Then, add chicory extract at concentrations of 0, 5, 10, 25 and 50 μg / ml, respectively, and adjust the total volume to 100 μl with 10% FBS-DMEM medium to obtain 37 ° C. and 5% CO ₂ . Incubated for 24 hours at. H ₂ O ₂ was added to the culture solution at a concentration of 1 mM so that the total volume was 200 μl, which was then incubated for 60 minutes. After incubation, 10 μl of EzCytox kit was added, and after 1 hour, the absorbance was measured to determine the effect.

As can be seen in Figure 1, as a result of measuring the muscle cell survival rate of the chicory extract for muscle cell necrosis induced by 1 mM H ₂ O ₂ , the concentration of the chicory root extract is 50 from 0 μg / ml As it increased to μg / ml it was confirmed that the cell viability was recovered in a concentration-dependent manner. Accordingly, it can be seen that the effect of preventing muscle cell necrosis is increased depending on the concentration of chicory extract.

(3-2) Effects of Chicory Extracts on H ₂ O ₂ Induced Muscle Cell Necrosis and Comparison with Positive Control

In order to examine the effect of chicory extract on oxidative stress, H ₂ O ₂ was treated to normal muscle cells (C ₂ C ₁₂ ). N -Acetyl cysteine (NAC) was used as a positive control to evaluate the effect of chicory extract. Cell survival rate after H ₂ O ₂ treatment was 28.80 ± 1.65%. However, treatment with chicory extract was found to increase cell viability in a concentration-dependent manner. In particular, when the cells were treated with chicory stem extract at 5, 10, 25 and 50 μg / mL, cell viability was 29.26 ± 8.38, 35.44 ± 7.46, 49.48 ± 8.01, 50.57 ± 1.93%, respectively. 33.18 ± 4.07, 51.84 ± 6.70, 109.80 ± 19.86, 145.29 ± 9.75%.

N -acetyl cysteine (NAC) was used as a positive control to evaluate the effect of chicory extract on cellular response to stress. Chicory root extract was found to reduce the reduction of cell survival of H ₂ O ₂ -induced normal muscle cells (C ₂ C ₁₂ ) as compared to antioxidants (see FIG. 2).

Although no data was shown, each extract 5, 10, 25 and 50 μg / mL were treated with normal muscle cells (C ₂ C ₁₂ ) without H ₂ O ₂ , and no cytotoxicity was observed.

(4) cytotoxicity measurement

Toxicity of chicory extract was measured using EzCytox kit. That is, normal muscle cells (C ₂ C ₁₂ ) were dispensed to 96 × plate 5 × 10 ⁴ cells / well. After incubation in an incubator at 37 ° C., 5% CO ₂ , chicory extracts (root extract and stem extract) of various concentrations of 0, 5, 10, 25, and 50 μg / ml were added and incubated for 48 hours. The cytotoxicity of chicory extract was determined using EzCytox kit on normal muscle cells cultured for 48 hours.

The results of measuring cytotoxicity in cultured normal muscle cells to which chicory extract was added are shown in FIG. 3. When chicory extract was not added, the survival rate was 100%, and in the addition group to which chicory extract was added, the survival rate was similar to that of the non-addition group. Therefore, it can be seen that the chicory extract used in this experiment does not show toxicity to cultured muscle cells.

(5-1) Effect of chicory extract on myocyte apoptosis by H ₂ O ₂

In order to observe the effect of chicory extract on apoptosis according to the concentration of H ₂ O ₂ , firstly put the muscle cells in the cover glass to 5 × 10 ⁴ cells / well and incubated in a CO ₂ incubator, and then the concentration of chicory extract 25 , 50 μg / ml each was added, followed by incubation for 24 hours at 37 ° C. and 5% CO ₂ in 10% FBS-DMEM medium. H ₂ O ₂ was added to the culture solution at a concentration of 1 mM, followed by another 60 minutes of incubation, and then the effect was determined after DAPI staining.

As a result of measuring the apoptosis of normal muscle cells according to H ₂ O ₂ as shown in Figure 4, it can be seen that the apoptosis of muscle cells is induced by H ₂ O ₂ . As a result of measuring the ability of chicory extract to prevent muscle cell apoptosis, it can be seen that the chicory extract added group prevents apoptosis.

(5-2) Cytotoxicity Measurement and Comparison with Positive Control

The effect of chicory root extract on cell death was tested and compared with NAC as a positive control. The percentage of apoptotic cells killed when treated with H ₂ O ₂ alone was 19.04 ± 6.17%, whereas the percentage of cells killed when treated with chicory root extract at 25 or 50 μg / mL was 12.96 ±, respectively. Decreases to 2.52 and 5.56 ± 0.62%. This shows that chicory root extract significantly reduces cell death in a concentration-dependent manner (see FIG. 5).

(6) measuring the effect on ceramide levels

Ceramide is involved in the regulation of cell death and acts as a lipid mediator of cellular stress responses. Ceramide levels in cells are up-regulated by various kinds of stress conditions. Hsp 70 is known to block apoptosis by blocking caspase-3 and SAPK / JNK activation and inhibiting the SAPK / JNK signal cascade in ceramide-induced apoptosis.

Experiments were conducted to determine the effects of chicory root extract on endogenous ceramide levels associated with oxidative stress induced normal muscle cell (C ₂ C ₁₂ ) damage. Normal muscle cells (C ₂ C ₁₂ ) were incubated in 96-well plates until confluent, then replaced with serum-free medium, divided into medium with and without chicory extract (0-50 μg / ml). . After 24 hours pre-incubation, H ₂ O ₂ (1 mM) was treated for 1 hour. Ethanol was used to extract lipids containing C17-ceramide from normal muscle cell (C ₂ C ₁₂ ) pellets and used as an internal standard. The released C17 and C18 sphingosine was stained with OPA fluorescent die and analyzed by HPLC. Each measurement represents the mean ± SD of three experiments, each performed three times. (* p <0.05, ** p <0.01)

HPLC analysis after treatment with H ₂ O ₂ showed an increase in the content of endogenous ceramide. On the other hand, when pretreated with chicory root extract, the ceramide content was found to be maintained at the control level (see FIG. 6).

(7) Hsp 70 protective effect experiment

The protective effect of Hsp 70 on cells is associated with the inhibition of apoptosis. Several studies have shown that Hsp 70 protects cells from stress-induced apoptosis and that chicory root extracts can increase Hsp 70 production and reduce normal muscle cell (C ₂ C ₁₂ ) death. Stand up and the experiment was performed.

Normal muscle cells (C ₂ C ₁₂ ) are incubated in 6-well plates until confluent, medium is replaced with serum-free medium, divided into medium with chicory extract and NAC (2 mM) for 24 hours. Incubated. Normal muscle cells (C ₂ C ₁₂ ) were lysed by treatment with 1 mM H ₂ O ₂ for 1 hour and the proteins in the lysates were analyzed by SDS-PAGE. Band intensities were quantified using an Image J densitometer. The data is the mean (± SD) of Hsp70 / β-actin percentages obtained from three independent experiments, each performed three times.

Western blot analysis of Hsp 70 was performed and the expression of Hsp 70 was increased in the chicory root extract treated group (see FIG. 7). Experimental results showed that apoptosis was markedly decreased by treatment with chicory root extract, Hsp 70 expression was increased, and Hsp 70 prevented apoptosis of H ₂ O ₂ -induced normal muscle cells (C ₂ C ₁₂ ). It is likely to play an important role.

The pharmaceutical composition and the food composition containing the chicory extract according to the present invention can be usefully used for the purpose of preventing, improving or treating muscle atrophy by inhibiting myocyte damage, necrosis and apoptosis.

Claims (15)

Pharmaceutical composition for preventing or treating muscle atrophy comprising chicory extract as an active ingredient. The method of claim 1, Chicory extract is a pharmaceutical composition for preventing or treating muscular atrophy, which is an extract of chicory flowers, leaves, branches, berries, roots, stems, seeds or mixtures thereof. The method of claim 1, Chicory extract is a stem or root extract of chicory pharmaceutical composition for muscular atrophy prevention or treatment. The method of claim 1, Muscular atrophy is a pharmaceutical composition for preventing or treating muscle atrophy, which is muscle atrophy due to loss of muscle tissue caused by not using muscle, muscle atrophy due to a disease of the muscle itself, or muscle atrophy due to nerve damage that governs the muscle. The method of claim 1, Chicory extract is a pharmaceutical composition for preventing or treating muscular dystrophy that is extracted with a solvent selected from water, organic solvents or mixtures thereof. The method of claim 1, Chicory extract is a pharmaceutical composition for preventing or treating muscular atrophy which is extracted with a solvent selected from water or C _1-4 lower alcohol or a mixture thereof. Food composition for preventing or improving muscular dystrophy comprising chicory extract as an active ingredient. The method of claim 7, wherein Chicory extract is a food composition for preventing or improving muscle atrophy which is an extract of chicory flowers, leaves, branches, berries, roots, stems, seeds or mixtures thereof. The method of claim 7, wherein Chicory extract is a stem or root extract of chicory food composition for preventing or improving muscular dystrophy. The method of claim 7, wherein Muscular atrophy is a muscle composition caused by the loss of muscle tissue caused by not using the muscle, muscle atrophy due to the disease of the muscle itself or muscle atrophy caused by damage to the nerves that control the muscle. The method of claim 7, wherein Chicory extract is a food composition for preventing or improving muscular dystrophy that is extracted with a solvent selected from water, organic solvents or mixtures thereof. The method of claim 7, wherein Chicory extract is a food composition for preventing or improving muscular dystrophy that is extracted with a solvent selected from water, organic solvents or mixtures thereof. The method of claim 7, wherein Chicory extract is a food composition for preventing or improving muscular dystrophy that is extracted with a solvent selected from water or C _1-4 lower alcohol or a mixture thereof. The method of claim 7, wherein Food composition is a food composition for preventing or improving muscle atrophy that is contained in space foods for inhibiting muscle aging or motor function due to space life. For preventing or treating muscular atrophy of chicory extract.

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