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WO2015070701A1 - Carte à bandelette réactive - Google Patents

Carte à bandelette réactive Download PDF

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Publication number
WO2015070701A1
WO2015070701A1 PCT/CN2014/089611 CN2014089611W WO2015070701A1 WO 2015070701 A1 WO2015070701 A1 WO 2015070701A1 CN 2014089611 W CN2014089611 W CN 2014089611W WO 2015070701 A1 WO2015070701 A1 WO 2015070701A1
Authority
WO
WIPO (PCT)
Prior art keywords
test strip
sample
strip
test
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2014/089611
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English (en)
Chinese (zh)
Inventor
马义才
顾敏
马灵
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CHENGDU LINGYU BIOTECHNOLOGY Co Ltd
Original Assignee
CHENGDU LINGYU BIOTECHNOLOGY Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CHENGDU LINGYU BIOTECHNOLOGY Co Ltd filed Critical CHENGDU LINGYU BIOTECHNOLOGY Co Ltd
Publication of WO2015070701A1 publication Critical patent/WO2015070701A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases

Definitions

  • the invention belongs to the field of in vitro diagnosis, and particularly relates to a test information capable of filtering a sample by itself, storing a standard curve or coefficient parameter of a test object with a storage medium 15, and combining the instrument with the signal detection function to quickly detect a single sample.
  • Component or multi-component strip card
  • Immunochromatographic strip technology which is mainly represented by colloidal gold strips, has been widely used in various aspects of immunoassays.
  • the technology is simple and fast, and results are available in minutes.
  • this technology has the following shortcomings:
  • red blood cells in the whole blood sample will prevent the smooth migration of the reactants on the test strip.
  • the red blood cell color (red) is similar to or the same as the colloidal gold display color (light red or purple), which may interfere with the detection result judgment.
  • Red blood cells interfere with many diagnostic assays. For example, in the detection of analytes, red blood cells may inhibit the binding between the paired components of specific structures; red blood cells have enzymatic activity, which may interfere with the detection signal.
  • ZL03217482.9 discloses a technical solution for adding an erythrocyte filtration membrane between a colloidal gold conjugate of a colloidal gold test strip and a nitrocellulose membrane in an attempt to overcome the above-mentioned interference effect of red blood cells.
  • the use of the standard curve of the test substance to quantify the concentration of the test substance is the main method for quantifying the concentration of the sample in the current detection field.
  • the standard curve is technically strong and the process is complicated, and it is inseparable from professional technicians.
  • Recently developed storage media in the information field such as RFID tags (also known as radio frequency identification tags), IC chips, magnetic codes, barcodes, etc., for information access identification is now widely used in computers, communications, electronics, commerce, transportation Control management and other fields.
  • RFID tags small size, large storage capacity, identification without manual intervention, is an ideal means of information access identification, but few people use it in biomedical testing.
  • the present invention provides a test information capable of filtering a sample by itself (the sample can be filtered when the whole blood is filtered), and using the self-contained storage medium 15 to store a standard curve or coefficient parameter of the test object. (The operator does not need to make the standard curve of the test object when testing the sample.)
  • the test piece of single-component or multi-component quantitative (qualitative/semi-quantitative) test can be quickly realized.
  • the invention is used for sample detection, and has the characteristics of simple and rapid, high sensitivity and objective result.
  • the test strip card of the present invention comprises a cartridge 3 and a test strip 2 therein.
  • the test strip 2 includes a sample pad 4, a filter pad 5, a marker pad 6, an analysis film 9, and an absorbent pad 10 which are sequentially attached to the substrate 1 by laps.
  • the analysis membrane 9 has a detection strip 7 and a quality control strip 8.
  • Card box The upper case of 3 is provided with a sample hole 12 at a position corresponding to the test strip 4 of the test strip.
  • the upper casing of the cartridge 3 is provided with a detection window 13 at a position corresponding to the test strip analysis film 9.
  • a storage medium 15 is also mounted on the cartridge 3.
  • the filter mat 5 of the test strip 2 includes a blood filter membrane.
  • the marker pad 6 of the test strip 2 is coated with a single specific molecule associated with a target analyte detected by a single marker, or a specific one associated with each target analyte coated with a marker corresponding to a different marker. a mixture of molecules.
  • the test strip 7 of the test strip 2 may be one piece or a plurality of strips.
  • the test strip 7 is coated with another specific molecule associated with detection of a target analyte, or a mixture of another specific molecule coated with detection of each target analyte.
  • the quality control strip 8 of the test strip 2 is coated with a quality control.
  • the control includes a secondary antibody.
  • the storage medium 15 on the cartridge 3 includes, but is not limited to, an RFID tag, an IC chip, a magnetic code, or a barcode.
  • the storage medium 15 stores the standard curve or coefficient parameter of the sample for quantitative sample concentration of the same batch test strip 2, the reference monitoring value of the test strip quality control belt signal, the test strip batch number, the test strip validity period, the storage medium password , clinical indicator reference value, test strip manufacturer information, etc., and can read the object identification information, tester information, sample name, sample number, date of detection, test results and other information.
  • the standard curve of the test object stored in the storage medium 15 can be selected in various forms, including but not limited to the following curves: the correspondence between the concentration of the sample standard series and the A detection zone /A quality control zone Relationship curve; or, the relationship between the concentration of the sample standard series and the A detection zone / (A detection zone + A quality control zone ).
  • the A detection zone is the signal intensity of the detection zone measured by the concentration of the standard product of the test object
  • the A control zone is the signal intensity of the quality control zone measured by the concentration of the standard product of the test object.
  • a test strip reaction end point indicating label 11 can be overlapped.
  • the test strip reaction end point of the cartridge 3 indicates that the label 11 is open corresponding to the observation window 14.
  • the strip reaction end point indicating label 11 includes, but is not limited to, a pH test paper having a discoloration range of 5-9.
  • the marking pad 6 of the test strip 2 of the present invention is a glass fiber membrane;
  • the analytical membrane 9 of the test strip 2 is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose/cellulose acetate mixed membrane;
  • the backing 1 is a polyester or plastic plate;
  • the cartridge 3 is made of polyester, plastic, hard paper material, or other material.
  • the test strip 2 of the present invention is any test strip, including but not limited to a quantum dot label test strip, a colloidal gold label test strip, a colloidal selenium label strip, an upconversion phosphor label strip, and a nano rare earth fluorescent complex labeling test. Strips, nano-magnetic particle labeling strips, time-resolved chromatographic strips, chemiluminescent strips.
  • test strip of the present invention When the test strip of the present invention is used for sample detection, a liquid sample is applied to the sample well 12 of the test strip card.
  • the liquid sample of the sample obtained by the sample pad 4 through the sample hole 12 is slowly pulled toward the rear end of the test strip 2 by capillary siphoning of the microporous filter under the pulling of the absorbent pad 10 at the other end of the test strip 2 (
  • the test strip reaction is completed by the filter pad 5 reaching the mark pad 6 first, and reacting with the mark conjugate of the mark pad 6 to continue the migration to the test tape 7 of the analysis film 9 and the control tape 8).
  • the liquid sample is filtered in the filter pad area 5 after the test strip source assembly (sample pad 4) to prevent interference in the sample from interfering with the test strip detection reaction immediately after the filter pad 5.
  • the test strip filter pad 5 will prevent the red blood cells in the blood from passing through and only allow the serum to be filtered, and the filter pad 5 serves to separate the serum (red blood cells can not only Prevents the smooth migration of the sample on the test strip 2, and when the sample is tested with a colloidal gold test strip, the red color of the red blood cells will be affected by the red or magenta color displayed by the colloidal gold mark on the test strip.
  • test strip 2 When the test strip 2 is used for qualitative test of the sample, it will affect the observation and judgment of the test result; when the test strip 2 is used for quantitative test of the sample, it will affect the accurate measurement of the optical density value of the test instrument, thus affecting Quantification of sample concentration).
  • the test strip of the present invention is used by an instrument having a signal detecting function (such as a reading strip) after the strip test object completes the test strip reaction.
  • the instrument, the fluorescence detector, the magnetic signal detector, etc. collect the characteristic signal of the detection zone 7 and the quality control zone 8 of the test strip 2 in the test strip and combine with the standard curve of the test object read from the storage medium 15 at the same time.
  • the coefficient parameter to quantitatively obtain the single component or multi-component concentration of the sample; or, the sample component is qualitatively or semi-quantitatively determined according to the display condition of the test strip 7 and the control strip 8 of the strip card.
  • the sample to be tested of the test strip card of the present invention may be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretions or other liquid samples or viscous samples, wherein the clinical samples include infectious diseases, Samples of hormones, cardiovascular diseases, tumors, cancer, diabetes, autoimmune diseases, etc., non-clinical samples including food testing, environmental pollution testing, pesticide residue testing, biological contamination testing, biological agent testing, veterinary testing, drugs Samples such as detection.
  • the present invention has the following beneficial effects:
  • the sample can be filtered to reduce the interference effect of the components affected by the sample detection.
  • the sample to be tested is a whole blood sample
  • the whole blood can be directly detected.
  • the existing test strip technology detects blood samples, it is necessary to separate the serum by using equipment such as a centrifuge or a centrifuge tube, and then perform detection, and the whole blood sample cannot be detected.
  • a storage medium 15 is mounted on the test strip card of the present invention, and the storage medium 15 stores detection information such as a standard curve or a coefficient parameter of the test object.
  • detection information such as a standard curve or a coefficient parameter of the test object.
  • Quantum Dots are semiconductor nanocrystals with excellent spectral characteristics and photochemical stability developed in the 1990s. They have high fluorescence emission efficiency, wide excitation line range, narrow emission line range, and fluorescence. It has a long life, large Stokes displacement, similar particle size to biomolecules, and multi-functionality after surface modification. Quantum dots of different particle sizes, types and structures can produce sustained fluorescence peak spectra with different characteristic wavelengths, and the characteristic wavelength fluorescence peak spectra produced by the quantum dot mixture do not overlap.
  • the corresponding reaction molecules of the test object are respectively labeled by using different quantum dots, and the mixture is coated on the test strip of the test strip of the present invention to react with the sample to be tested, and the specific fluorescent signal of the test strip can be measured within a few minutes.
  • the existing test strip technology can only qualitatively detect samples, and can not achieve quantitative detection of samples, and can not achieve quantitative detection of multi-component samples.
  • the reagents and materials in the test strip are not involved in activity inactivation and can be stored at room temperature for a long time.
  • Figure 1 is a top plan view of a preferred embodiment of the test strip card of the present invention (showing a test line in the inner test strip)
  • Figure 2 is a side view showing the structure of the test strip in the cartridge of the first preferred embodiment of the strip card of the present invention (the test strip adopts a test line)
  • Figure 3 is a top plan view of a preferred embodiment of the test strip card of the present invention (showing a plurality of test lines in the test strip)
  • Figure 4 is a side view showing the structure of the test strip in the cartridge of the first preferred embodiment of the test strip card of the present invention (the test strip adopts a plurality of test lines)
  • Figure 5 is a top plan view of another preferred embodiment of the test strip of the present invention (showing a test line in the test strip)
  • Figure 6 is a side view showing the structure of the test strip in another embodiment of the test strip card of the present invention (the test strip adopts a test line)
  • Figure 7 is a top plan view of another preferred embodiment of the test strip card of the present invention (showing a plurality of test lines in the test strip)
  • FIG. 8 is a side view structural view of a test strip in a cartridge according to another preferred embodiment of the test strip card of the present invention (the test strip adopts a plurality of detection lines)
  • test strip 1, underlay, 2, test strip, 3, card box, 4, sample pad, 5, filter pad, 6, marker pad, 7, detection tape, 8, quality control tape, 9, analysis of the film, 10, absorbent pad, 11, test strip reaction end indication label, 12, sample hole, 13, detection window, 14, observation window, 15, storage medium.
  • Embodiment 1 is one of the preferred embodiments of the test strip card of the present invention.
  • the strip card of the preferred embodiment includes a cartridge 3 and a strip 2 therein.
  • the test strip 2 includes a sample pad 4, a filter pad 5, a marker pad 6, an analysis film 9, and an absorbent pad 10 which are sequentially attached to the substrate 1.
  • the analysis membrane 9 has a detection strip 7 and a quality control strip 8.
  • the upper surface of the cartridge 3 is provided with a sample insertion hole 12 at a position corresponding to the test strip 4 of the test strip.
  • the upper casing of the cartridge 3 is provided with a detection window 13 at a position corresponding to the test strip analysis film 9.
  • a storage medium 15 is mounted on the cartridge 3.
  • the filter mat 5 of the test strip 2 includes a blood filter membrane.
  • the marker pad 6 of the test strip 2 is coated with a single specific molecule associated with a target analyte detected by a single marker, or a specific one associated with each target analyte coated with a marker corresponding to a different marker. a mixture of molecules.
  • the test strip 7 of the test strip 2 may be one (shown in Figures 1 and 2) or a plurality of strips (shown in Figures 3 and 4).
  • the test strip 7 is coated with another specific molecule associated with detection of a target analyte, or a mixture of another specific molecule coated with detection of each target analyte.
  • the quality control strip 8 of the test strip 2 is coated with a quality control.
  • the control includes a secondary antibody.
  • the storage medium 15 on the cartridge 3 includes, but is not limited to, an RFID tag, an IC chip, a magnetic code, or a barcode.
  • the storage medium 15 stores the standard curve or coefficient parameter of the sample for quantitative sample concentration of the same batch test strip 2, the reference monitoring value of the test strip quality control belt signal, the test strip batch number, the test strip validity period, the storage medium password , clinical indicator reference value, test strip manufacturer information, etc., and can read the object identification information, tester information, sample name, sample number, date of detection, test results and other information.
  • the standard curve of the test object stored in the storage medium 15 can be selected in various forms, including but not limited to the following curves: the correspondence between the concentration of the sample standard series and the A detection zone /A quality control zone Relationship curve; or, the relationship between the concentration of the sample standard series and the A detection zone / (A detection zone + A quality control zone ).
  • the A detection zone is the signal intensity of the detection zone measured by the concentration of the standard product of the test object
  • the A control zone is the signal intensity of the quality control zone measured by the concentration of the standard product of the test object.
  • the marking pad 6 of the test strip 2 is a glass fiber membrane; the analysis membrane 9 of the test strip 2 is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose/cellulose acetate mixed membrane; the bottom of the test strip 2
  • the liner 1 is a polyester or plastic plate; the cartridge 3 is made of polyester, plastic, hard paper material, or other material.
  • the test strip 2 is any test strip, which includes, but is not limited to, a quantum dot label test strip, a colloidal gold label test strip, a colloidal selenium label strip, an upconversion phosphor label strip, a nano rare earth fluorescent complex label strip, Nano magnetic particle labeling strip, time-resolved chromatographic strip, chemiluminescent strip.
  • the liquid sample is applied to the sample hole 12 of the test strip card, and after the inner test strip 2 completes the reaction, the instrument with signal detection function (such as a reading instrument, fluorescence)
  • the instrument with signal detection function such as a reading instrument, fluorescence
  • the detector, the magnetic signal detector, and the like collect the characteristic signals of the detection zone 7 and the quality control zone 8 of the test strip 2 in the test strip and combine with the test object to read the standard curve of the test object simultaneously from the test strip storage medium 15 Or the coefficient parameter to quantitatively obtain the single component or multi-component concentration of the sample; or, the sample component is qualitatively or semi-quantitatively determined according to the display condition of the test strip 7 and the control strip 8 of the strip card.
  • the sample to be tested of the test strip card may be from clinical or non-clinical blood, body fluid, urine, saliva, reproductive tract Exudates or other liquid samples or viscous samples, including clinical samples including infectious diseases, hormones, cardiovascular diseases, tumors, cancer, diabetes, autoimmune diseases, etc., non-clinical samples including food testing, environmental pollution Samples such as testing, pesticide residue testing, bio-contamination testing, biologics testing, veterinary testing, and drug testing.
  • Embodiment 2 is another preferred embodiment of the test strip card of the present invention.
  • the strip card of the preferred embodiment includes a cartridge 3 and a strip 2 therein.
  • the test strip 2 includes a sample pad 4, a filter pad 5, a marker pad 6, an analysis film 9, an absorbent pad 10, and a test strip reaction end point label 11 which are sequentially attached to the substrate 1 by laps.
  • the analysis membrane 9 has a detection strip 7 and a quality control strip 8.
  • the upper surface of the cartridge 3 is provided with a sample insertion hole 12 at a position corresponding to the test strip 4 of the test strip.
  • the upper casing of the cartridge 3 is provided with a detection window 13 at a position corresponding to the test strip analysis film 9.
  • the upper casing of the cartridge 3 is provided with an observation window 14 at a position corresponding to the test strip end point indicating label 11.
  • a storage medium 15 is mounted on the cartridge 3.
  • the filter mat 5 of the test strip 2 includes a blood filter membrane.
  • the marker pad 6 of the test strip 2 is coated with a single specific molecule associated with a target analyte detected by a single marker, or a specific one associated with each target analyte coated with a marker corresponding to a different marker. a mixture of molecules.
  • the test strip 7 of the test strip 2 may be one (shown in FIGS. 5 and 6) or a plurality of strips (shown in FIGS. 7 and 8).
  • the test strip 7 is coated with another specific molecule associated with detection of a target analyte, or a mixture of another specific molecule coated with detection of each target analyte.
  • the quality control strip 8 of the test strip 2 is coated with a quality control.
  • the control includes a secondary antibody.
  • the strip reaction end point indicating label 11 includes, but is not limited to, a pH test paper having a discoloration range of 5-9.
  • the test strip reaction end point on the test strip indicates that the label 11 can be colored according to the pH condition of the test strip reaction, to indicate whether the test strip reactant has sufficiently oozing through the test strip 7 and the quality control strip 8, prompting the test Whether the strip reaction is sufficient and whether the test result is valid.
  • the storage medium 15 on the cartridge 3 includes, but is not limited to, an RFID tag, an IC chip, a magnetic code, or a barcode.
  • the storage medium 15 stores the standard curve or coefficient parameter of the sample for quantitative sample concentration of the same batch test strip 2, the reference monitoring value of the test strip quality control belt signal, the test strip batch number, the test strip validity period, the storage medium password , clinical indicator reference value, test strip manufacturer information, etc., and can read the object identification information, tester information, sample name, sample number, date of detection, test results and other information.
  • the standard curve of the test object stored in the storage medium 15 can be selected in various forms, including but not limited to the following curves: the correspondence between the concentration of the sample standard series and the A detection zone /A quality control zone Relationship curve; or, the relationship between the concentration of the sample standard series and the A detection zone / (A detection zone + A quality control zone ).
  • the A detection zone is the signal intensity of the detection zone measured by the concentration of the standard product of the test object
  • the A control zone is the signal intensity of the quality control zone measured by the concentration of the standard product of the test object.
  • the marking pad 6 of the test strip 2 is a glass fiber membrane; the analysis membrane 9 of the test strip 2 is a nitrocellulose membrane, a nylon membrane, or a nitrocellulose/cellulose acetate mixed membrane; the bottom of the test strip 2
  • the liner 1 is a polyester or plastic plate; the cartridge 3 is made of polyester, plastic, hard paper material, or other material.
  • the test strip 2 is any test strip, which includes, but is not limited to, a quantum dot label test strip, a colloidal gold label test strip, a colloidal selenium label strip, an upconversion phosphor label strip, a nano rare earth fluorescent complex label strip, Nano magnetic particle labeling strip, time-resolved chromatographic strip, chemiluminescent strip.
  • the liquid sample is applied to the sample insertion hole 12 of the test strip card, and the test strip reaction end point label 11 of the inner test strip 2 indicates that the test strip 2 completes the reaction, and has
  • the instrument for signal detection function (such as reading instrument, fluorescence detector, magnetic signal detector, etc.) collects the detection band 7 of the test strip 2 in the test strip card and the characteristic signal of the quality control belt 8 and combines The instrument quantitatively obtains the single component or multi-component concentration of the sample from the standard curve or coefficient parameter of the test object read from the test strip storage medium 15; or, according to the test strip of the test strip card 7
  • the sample components are qualitatively or semi-quantitatively detected with the display of the control strip 8.
  • the sample to be tested of the test strip card may be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretions or other liquid samples or viscous samples, wherein the clinical samples include infectious diseases, hormones, Samples of cardiovascular disease, cancer, cancer, diabetes, autoimmune diseases, etc., non-clinical samples including food testing, environmental pollution testing, pesticide residue testing, biological contamination testing, biological agent testing, veterinary testing, drug testing, etc.
  • the test strip card of the present invention may have other improvements.
  • the storage medium 15 storing the detection information such as the standard curve of the test object may be installed on the cartridge 3 of the test strip card.
  • the test strip in the cartridge 3 can also be expanded and replaced with a biochip (including an antigen-antibody chip, a protein chip, a nucleic acid chip, a microfluidic chip), etc. Wait. Therefore, any other technical solutions formed by any equivalent replacement or equivalent transformation of the test strip of the present invention fall within the scope of protection of the present invention.

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Abstract

La présente invention concerne une carte à bandelette réactive permettant de filtrer des échantillons et dotée d'un moyen de stockage (15) permettant de stocker une courbe standard d'objet testée, un paramètre de coefficient ou d'autres informations de détection. La carte à bandelette réactive comprend un boîtier de carte (3) et une bandelette réactive (2) présente dans le boîtier de carte (3). Le moyen de stockage (15) est monté sur le boîtier de carte (3). Lorsqu'un échantillon est détecté, un instrument doté d'une fonction de détection de signal acquiert des signaux de caractéristique d'une courroie de détection (7) et d'une courroie de contrôle de qualité (8) de la bandelette réactive (2), puis calcule la concentration mono-composant ou multi-composants d'un échantillon en association avec la courbe standard d'objet testé ou le paramètre de coefficient lu simultanément depuis le moyen de stockage (15) par l'instrument. La carte à bandelette réactive offre les caractéristiques suivantes : simplicité et rapidité, sensibilité élevée, résultats objectifs et flexibilité d'utilisation.
PCT/CN2014/089611 2013-11-13 2014-10-27 Carte à bandelette réactive Ceased WO2015070701A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201310565069.4 2013-11-13
CN201310565069.4A CN103630683A (zh) 2013-11-13 2013-11-13 一种试条卡

Publications (1)

Publication Number Publication Date
WO2015070701A1 true WO2015070701A1 (fr) 2015-05-21

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PCT/CN2014/089611 Ceased WO2015070701A1 (fr) 2013-11-13 2014-10-27 Carte à bandelette réactive

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CN (1) CN103630683A (fr)
WO (1) WO2015070701A1 (fr)

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US20200141939A1 (en) * 2016-10-10 2020-05-07 Dongha Bang Apparatus and method for detecting canine cancer
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CN115046971A (zh) * 2022-05-05 2022-09-13 中国科学院苏州生物医学工程技术研究所 全血中百草枯快检电子卡
CN115819591A (zh) * 2022-11-25 2023-03-21 南京珀尔泰生物技术有限公司 抗红细胞膜抗体及其应用

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7157048B2 (en) * 1993-05-19 2007-01-02 Sira Technologies, Inc. Detection of contaminants
CN101452000A (zh) * 2008-12-30 2009-06-10 扬州大学 快速检测牛γ干扰素的试剂条及其制备方法
CN102520193A (zh) * 2011-12-29 2012-06-27 深圳康美生物科技股份有限公司 一种定量检测人心肌肌钙蛋白i的荧光免疫层析方法及其试剂盒
CN102539785A (zh) * 2011-12-29 2012-07-04 深圳康美生物科技股份有限公司 一种全程定量检测c-反应蛋白的荧光免疫层析方法及其试剂盒
CN102565422A (zh) * 2011-12-29 2012-07-11 深圳康美生物科技股份有限公司 一种定量检测心肌肌钙蛋白t的荧光免疫层析方法及其试剂盒
CN102565423A (zh) * 2011-12-29 2012-07-11 深圳康美生物科技股份有限公司 一种定量检测氮末端脑钠肽的荧光免疫层析方法及其试剂盒
CN202735347U (zh) * 2012-08-23 2013-02-13 南京基蛋生物科技有限公司 新型临床检测免疫层析试纸条
CN103091486A (zh) * 2011-11-01 2013-05-08 马义才 一种试条检测系统
CN103630683A (zh) * 2013-11-13 2014-03-12 成都领御生物技术有限公司 一种试条卡
CN103645314A (zh) * 2013-11-29 2014-03-19 电子科技大学 能自动过滤样品的试条卡
CN203759014U (zh) * 2013-11-14 2014-08-06 成都领御生物技术有限公司 一种试条卡
CN203759017U (zh) * 2013-11-13 2014-08-06 成都领御生物技术有限公司 一种试条卡

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2629040Y (zh) * 2003-05-26 2004-07-28 漳州市格林沃生物技术有限公司 改良的简捷快速疫病诊断试纸条
CN100582777C (zh) * 2004-06-02 2010-01-20 瑞莱诊断体系有限公司 定量侧向流系统和测定
CN201935921U (zh) * 2011-01-30 2011-08-17 南京基蛋生物科技有限公司 一种带有u型过滤结构及三层滤血膜的胶体金全血检测卡
CN202583195U (zh) * 2012-05-15 2012-12-05 南京基蛋生物医药有限公司 一种新型胶体金检测试纸条
CN202583196U (zh) * 2012-05-15 2012-12-05 南京基蛋生物医药有限公司 一种新型胶体金全血检测卡
CN203249919U (zh) * 2013-05-21 2013-10-23 北京健乃喜生物技术有限公司 一种设置有红细胞过滤滤纸的新型检测测试卡

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7157048B2 (en) * 1993-05-19 2007-01-02 Sira Technologies, Inc. Detection of contaminants
CN101452000A (zh) * 2008-12-30 2009-06-10 扬州大学 快速检测牛γ干扰素的试剂条及其制备方法
CN103091486A (zh) * 2011-11-01 2013-05-08 马义才 一种试条检测系统
CN102520193A (zh) * 2011-12-29 2012-06-27 深圳康美生物科技股份有限公司 一种定量检测人心肌肌钙蛋白i的荧光免疫层析方法及其试剂盒
CN102539785A (zh) * 2011-12-29 2012-07-04 深圳康美生物科技股份有限公司 一种全程定量检测c-反应蛋白的荧光免疫层析方法及其试剂盒
CN102565422A (zh) * 2011-12-29 2012-07-11 深圳康美生物科技股份有限公司 一种定量检测心肌肌钙蛋白t的荧光免疫层析方法及其试剂盒
CN102565423A (zh) * 2011-12-29 2012-07-11 深圳康美生物科技股份有限公司 一种定量检测氮末端脑钠肽的荧光免疫层析方法及其试剂盒
CN202735347U (zh) * 2012-08-23 2013-02-13 南京基蛋生物科技有限公司 新型临床检测免疫层析试纸条
CN103630683A (zh) * 2013-11-13 2014-03-12 成都领御生物技术有限公司 一种试条卡
CN203759017U (zh) * 2013-11-13 2014-08-06 成都领御生物技术有限公司 一种试条卡
CN203759014U (zh) * 2013-11-14 2014-08-06 成都领御生物技术有限公司 一种试条卡
CN103645314A (zh) * 2013-11-29 2014-03-19 电子科技大学 能自动过滤样品的试条卡

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110488024A (zh) * 2019-09-23 2019-11-22 北京纳晶生物科技有限公司 采用荧光技术检测多种待检测物质的方法及荧光检测产品
CN110488024B (zh) * 2019-09-23 2022-07-12 北京纳诺金生物科技有限公司 采用荧光技术检测多种待检测物质的方法及荧光检测产品

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