WO2015070386A1 - Dietary nutritional supplement for preventing elderly osteoarticular diseases - Google Patents

Abstract

Provided in the present invention is a dietary nutritional supplement for preventing elderly osteoarticular diseases, wherein the dietary nutritional supplement is obtained through the steps of: adding low-molecular-weight chondroitin sulfate to water at a ratio of 1:5 to be dissolved therein, adding a type II cartilage collagen peptide to water at a ratio of 1:5 to be dissolved therein, mixing the aforementioned two dissolved solutions at a mass ratio of 1:1-1:3, and homogenizing, concentrating, spray drying, granulating and packaging, wherein the homogenizing uses secondary homogenizing, and the first homogenizing pressure is 20-25 MPa and the homogenizing temperature is 40-50°C, while the second homogenizing pressure is 4-7 MPa, and the homogenizing temperature is 30-40°C.

Description

 Dietary nutritional supplement for preventing bone and joint diseases in middle-aged and elderly people

 Technical field

 The invention relates to a dietary nutritional supplement for preventing bone and joint diseases of middle-aged and elderly people, and belongs to the field of bioengineering.

 Background technique

Because middle-aged and elderly people do not engage in or engage in violent or heavy physical activities such as running, jumping, and weight-bearing for a long time, joint function will gradually develop toward atrophy and retreat. The specific performance is: the soft tissue of the joint capsule, ligament, muscle and so on gradually shrinks, hardens and rigid; the joint activity decreases; the elasticity, toughness and damage resistance of the joint to adapt to strenuous exercise gradually weaken; the bone tissue exists to adapt to strenuous exercise The hardness, ie, bone density, calcium content, etc., gradually decreases. Osteoarticular diseases occur more and more frequently. Due to the poor metabolism of middle-aged and elderly people, the absorption capacity is weak. Traditionally, the health care products and medicines based on chondroitin sulfate, collagen and calcium, which are traditional to prevent bone and joint diseases, have high molecular weight and low absorption and utilization levels. The prevention of disease plays a very good role.

 Chondroitin sulfate (Chondroitin Sulfate (CS) is an acidic mucopolysaccharide found in connective tissue of humans and animals, mainly distributed in cartilage, bone, tendon, sarcolemma and blood vessel walls. As a component of proteoglycans, CS exists in intracellular granules, cell membranes, basement membranes, and extracellular matrices, and specifically binds to cytokines, adhesion factors, or their receptors through specific domains in the sugar chain. Important physiological functions are also closely related to many pathological processes. However, due to the large molecular weight of CS, many hydrophilic groups, and poor fat solubility, the in vivo absorption is incomplete, and the lack of bioavailability has been plaguing the large-scale application of CS drugs. Compared with ordinary CS, LMWCS is more conducive to absorption and utilization by the body due to the decrease of molecular weight, and has great application potential in medicinal, health care products and cosmetics.

There are different types of collagen, and dozens of them have been discovered so far. Type II is mainly produced by chondrocytes, and is mostly found in bones, joints, tendons and other tissues; the molecular weight of type II cartilage collagen affects the absorption rate and utilization rate of collagen, and the molecular weight most suitable for human body absorption is 1500-3000 Daltons. between. A molecular weight of less than 1500 Daltons leads to collagen instability, and a molecular weight of more than 3000 Daltons is not conducive to human absorption, especially for middle-aged and elderly people.

 Summary of the invention

The object of the present invention is to overcome the deficiencies of the prior art, and to provide a characteristic that the cartilage tissue of the middle-aged and the elderly is gradually atrophied, hardened and rigid, and susceptible to bone and joint diseases, and has an important role in preventing bone and joint diseases, and is easy to digest and absorb. Low-molecular-weight chondroitin and type II cartilage collagen peptide are used as raw materials to prepare a dietary supplement for preventing bone and joint diseases in middle-aged and elderly people.

In order to achieve the above object, the technical solution adopted by the present invention is: a dietary nutritional supplement for preventing bone and joint diseases of middle-aged and elderly people, and the low molecular weight chondroitin sulfate is dissolved in water at a ratio of 1:5, and the type II cartilage collagen peptide is pressed. 1:5 is dissolved in water, and the dissolved solution of the above two is mixed at a mass ratio of 1:1 to 3, and is prepared by homogenization, concentration, spray drying, granulation, and packaging, wherein homogenization is performed by secondary homogenization. The first homogenization pressure is 20-25MPa, the homogenization temperature is 40-50°C, the second homogenization pressure is 4-7MPa, and the homogenization temperature is 30-40°C.

Further, the low molecular chondroitin sulfate has a chondroitin disaccharide content of 98.5% or more and a molecular weight of 455-460, and is prepared by enzymatic hydrolysis of chondroitin sulfate by a fermentation liquid. The conditions for enzymatic hydrolysis of chondroitin sulfate by the fermentation broth are: enzymatic hydrolysis of chondroitin sulfate 0.1-0.6 kg per liter of fermentation broth, enzymatic hydrolysis time 5-8 h, enzymatic hydrolysis temperature 30-45 ° C, and enzymatic hydrolysis pH 6-8.

Further, the fermentation broth is a fermentation broth of Arthrobacter sulphate, and the medium component is w/v: chondroitin sulfate 0.7-0.9%, KH ₂ PO ₄ 0.01-0.03%, yeast dipping powder 0.5-0.7% MgSO ₄ ·7H ₂ 0 content 0.7-0.9%, pH 5-7; fermentation conditions: 1.5% v / v inoculum seed fungus into the fermenter at 20-35 ° C, 100-300r / Min oscillation culture for 48-96h.

 Further, the chondroitin sulfate A content in the chondroitin sulfate disaccharide is 70-80%, and the chondroitin sulfate C content is 10-15%.

Further, the type II cartilage collagen peptide is prepared by biological extraction of cartilage of pig, chicken, duck or cow, the collagen peptide content is above 85%, the molecular weight of collagen peptide is below 5KDa, and the collagen peptide of 1.5-3KDa accounts for 60%. -80%.

The beneficial effects of the invention are as follows: the nutritional supplement has good absorption, plays an important role in preventing bone and joint diseases of middle-aged and elderly people, and has convenient production process, short cycle and low production cost.

 detailed description

 Example 1

A dietary nutrient supplement for preventing bone and joint diseases of middle-aged and elderly people, the low-molecular-weight chondroitin sulfate is dissolved in water at 1:5, and the type II cartilage collagen peptide is dissolved in water at a ratio of 1:5, and the two solutions are dissolved. According to the mass ratio of 1:1~3, it is prepared by homogenization, concentration, spray drying, granulation and packaging. The homogenization adopts secondary homogenization, the first homogenization pressure is 20-25MPa, and the homogenization temperature 40-50 ° C, the second homogenization pressure 4-7MPa, homogenization temperature 30-40 ° C.

Further, the low molecular chondroitin sulfate has a chondroitin disaccharide content of 98.5% or more and a molecular weight of 455-460, and is prepared by enzymatic hydrolysis of chondroitin sulfate by a fermentation liquid. The conditions for enzymatic hydrolysis of chondroitin sulfate by the fermentation broth are: enzymatic hydrolysis of chondroitin sulfate 0.1-0.6 kg per liter of fermentation broth, enzymatic hydrolysis time 5-8 h, enzymatic hydrolysis temperature 30-45 ° C, and enzymatic hydrolysis pH 6-8.

Further, the fermentation broth is a fermentation broth of Arthrobacter sulphate, and the medium component is w/v: chondroitin sulfate 0.7-0.9%, KH ₂ PO ₄ 0.01-0.03%, yeast dipping powder 0.5-0.7% MgSO ₄ ·7H ₂ 0 content 0.7-0.9%, pH 5-7; fermentation conditions: 1.5% v / v inoculum seed fungus into the fermenter at 20-35 ° C, 100-300r / Min oscillation culture for 48-96h.

 Further, the chondroitin sulfate A content in the chondroitin sulfate disaccharide is 70-80%, and the chondroitin sulfate C content is 10-15%.

Further, the type II cartilage collagen peptide is prepared by biological extraction of cartilage of pig, chicken, duck or cow, the collagen peptide content is above 85%, the molecular weight of collagen peptide is below 5KDa, and the collagen peptide of 1.5-3KDa accounts for 60%. -80%.

 Example 2

Accurately weigh 100 kg of low molecular weight chondroitin sulfate and dissolve it into 500 kg of purified water. Among them, the low molecular weight chondroitin has a chondroitin disaccharide content of 96% or more, a molecular weight of 463, and a chondroitin sulfate A content of chondroitin disaccharide of 83%, which is obtained by bio-enzymatic extraction of porcine cartilage. Chondroitin sulfate, then chondroitin sulfate is produced by enzymatic hydrolysis of chondroitin sulfate lyase. Accurately weigh 300 kg of type II cartilage collagen peptide and dissolve it into 1500 kg of water. It is prepared by alkaline protease hydrolysis in pig cartilage without any infection. The collagen peptide content is above 87%, and the molecular weight of collagen peptide is below 5KDa. The collagen peptide of 1.5-3KDa accounts for 75%. The low molecular weight chondroitin sulfate solution and the type II cartilage collagen peptide solution are mixed and homogenized by a high pressure homogenizer. The first homogenization pressure is 20 MPa, the homogenization temperature is 40 ° C, and the second homogenization pressure is 4 MPa. The temperature is 30 °C. After homogenization, it is concentrated, spray-dried, granulated according to customer needs, and packaged into various dosage forms for sale. After taking a week for middle-aged and elderly people, bone density is significantly increased, which plays an important role in preventing bone and joint diseases.

 Example 3

Accurately weigh 1 kg of low molecular weight chondroitin sulfate and dissolve it into 5 kg of purified water. Among them, the low molecular weight chondroitin has a chondroitin disaccharide content of 97% or more, a molecular weight of 458, and a chondroitin sulfate A content of 85%. It is obtained by bio-enzymatic extraction of chicken sternum to obtain chondroitin sulfate, followed by sulfated cartilage. It is produced by enzymatic hydrolysis of chondroitin sulfate lyase. Accurately weigh 2 kg of type II cartilage collagen peptide and dissolve it into 10 kg of water. It is prepared by alkaline protease hydrolysis in chicken cartilage without any infection. The collagen peptide content is above 85%, and the molecular weight of collagen peptide is below 5KDa. The collagen peptide of 1.5-3KDa accounts for 69%. Mix all the low-molecular-weight chondroitin sulfate solution and all the type II cartilage collagen peptide solution, and homogenize by high-pressure homogenizer. The homogenization pressure is the first homogenization pressure 25MPa, and the homogenization temperature is 50°C. Secondary homogenization pressure 7 MPa, homogenization temperature 40 °C. After homogenization, it is concentrated, spray-dried, granulated according to customer needs, and packaged into various dosage forms for sale. After taking a week for middle-aged and elderly people, bone density is significantly increased, which plays an important role in preventing bone and joint diseases.

The above is only a specific embodiment of the present invention, and is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope of the present invention, and should be covered by the present invention. Within the scope of protection.

Claims (1)

1 , A dietary nutrient supplement for preventing bone and joint diseases of middle-aged and elderly people, the low-molecular-weight chondroitin sulfate is dissolved in water at 1:5, and the type II cartilage collagen peptide is dissolved in water at a ratio of 1:5, and the two solutions are dissolved. According to the mass ratio of 1:1~3, it is prepared by homogenization, concentration, spray drying, granulation and packaging. The homogenization adopts secondary homogenization, the first homogenization pressure is 20-25MPa, and the homogenization temperature 40-50 ° C, the second homogenization pressure 4-7MPa, homogenization temperature 30-40 ° C. A dietary supplement for preventing bone and joint diseases in middle-aged and elderly people according to claim 1, wherein: The low molecular chondroitin sulfate has a chondroitin disaccharide content of 98.5% or more and a molecular weight of 455-460, and is prepared by enzymatic hydrolysis of chondroitin sulfate by a fermentation broth. The condition of enzymatic hydrolysis of chondroitin sulfate by fermentation broth is to digest 0.1-0.6 kg of chondroitin sulfate per liter of fermentation broth, the enzymatic hydrolysis time is 5-8 h, the enzymatic hydrolysis temperature is 30-45 ° C, and the enzymatic hydrolysis pH is 6-8. The dietary supplement for preventing bone and joint diseases in middle-aged and elderly people according to claim 2, wherein: the fermentation liquid is a fermentation broth of Arthrobacter sulphate, and the medium component is w/v: sulfated cartilage. 0.7-0.9%, KH ₂ PO ₄ 0.01-0.03%, yeast dipped 0.5-0.7%, MgSO ₄ ·7H ₂ 0 content 0.7-0.9%, pH 5-7; fermentation conditions: 1.5% v / v Inoculation amount The seed broth was connected to the fermenter at 20-35 ° C, and incubated at 100-300 r/min for 48-96 h. A dietary supplement for preventing bone and joint diseases in middle-aged and elderly people according to claim 2, wherein: The chondroitin sulfate A content in the chondroitin sulfate disaccharide is 70-80%, and the chondroitin sulfate C content is 10-15%. A dietary supplement for preventing bone and joint diseases in middle-aged and elderly people according to claim 1, wherein: The type II cartilage collagen peptide is prepared by biological method of cartilage of pig, chicken, duck or cow, and the collagen peptide content is above 85%, the molecular weight of collagen peptide is below 5KDa, and the collagen peptide of 1.5-3KDa accounts for 60-80%. .