[go: up one dir, main page]

WO2015063540A1 - Microdispositif de type laboratoire sur puce pour identifier la sensibilité antibiotique au centre de soins des patients - Google Patents

Microdispositif de type laboratoire sur puce pour identifier la sensibilité antibiotique au centre de soins des patients Download PDF

Info

Publication number
WO2015063540A1
WO2015063540A1 PCT/IB2013/059726 IB2013059726W WO2015063540A1 WO 2015063540 A1 WO2015063540 A1 WO 2015063540A1 IB 2013059726 W IB2013059726 W IB 2013059726W WO 2015063540 A1 WO2015063540 A1 WO 2015063540A1
Authority
WO
WIPO (PCT)
Prior art keywords
micro
sample
antibiotic sensitivity
microdevice
micro device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2013/059726
Other languages
English (en)
Spanish (es)
Inventor
Sara DROGUETT BIZET
Mario SOTO AGUILERA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to PCT/IB2013/059726 priority Critical patent/WO2015063540A1/fr
Publication of WO2015063540A1 publication Critical patent/WO2015063540A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces

Definitions

  • the present invention relates to the clinical diagnostic industry, in particular with the use of micro fluidic devices to carry out such diagnoses, such as blood gas analysis, molecular biology based tests, use of point immunoassay. of patient care.
  • lab-on-chips consist of self-sufficient diagnostic platforms in which, in principle, all diagnostic actions can be carried out by mixing between the different reagents, which run through the device and react in suitable cameras, to provide simple readings, usually by visual inspection, about the diagnosis.
  • the concept of lab-on-chip, today in most situations corresponds actually to chip-on-lab devices, that is, a simple microsystem, but that needs a set of machines, pumps , readers, pre-existing support structures in the analysis laboratory, for proper operation.
  • Current trends are aimed at achieving greater autonomy of these fluidic micro devices so that they can be used easily at the point of patient care.
  • microsystems for total analysis are the so-called microsystems for total analysis or " ⁇ -TAS" ("micro total analysis systems"), which can be conceived as advanced labs-on-chips or with greater integration of functions, thanks to the use of thermo-opto-electro-mechanical components with which a greater number of physical-chemical domains is controlled and more precise answers or with more information are obtained.
  • ⁇ -TAS micro total analysis systems
  • Diagnostic Strips consist of microfluidic devices for diagnosis at the point of extended and simpler patient care, generally oriented towards “all / nothing" diagnoses of the type "the patient has an infection”, “pregnancy tests” and similar ones. They are usually made of paper or very economical materials (polymers) and act by immersion of one of its ends in the sample under study, which by diffusion or by capillarity reaches different areas of the test strip and activates a color change, in case of positive diagnosis. Recently it is being investigated in the step of the traditional qualitative diagnosis using diagnostic strips to diagnoses with certain quantification.
  • micro devices described by the prior art corresponds to the so-called fluidic cartridges and integrated platforms, which correspond to complex systems composed of a hardware (desktop machine) with its own software for control of the analysis process in which cartridges are introduced fluids prepared with all reagents (as if it were a printer ink cartridge) and with the sample to be processed. They have communication ports for integration into hospital information systems and are often used for complex diagnoses, usually in the fields of genetics, molecular biology and the like.
  • a circular type device is available, preferably made of medical grade polymer, cyclic olefin or other material which has a microcamera to deposit the sample to be treated.
  • microcamera there is a plurality of microchannels, through which the sample will spread to at least one microwell, in which the process of identifying antibiotic sensitivity is developed.
  • Each of the microwells contains an antibiotic, a culture medium and a chromophore substance.
  • the antibiotics to be used would be those of routine use in medical practice, within which you can preferably use Pipedimic acid, Cephalotoxin, Ciprofloxacin, Cotrimozaxol, Gentamicin, Nitrofurantoin, Ampicillin, which does not exclude the use of other antibiotics
  • Each microchannel and each microwell are independent of the others to avoid cross contamination, so that the sample that diffuses from the microcamera has a unidirectional flow.
  • micro device of the invention allows to identify antibiotic sensitivity at the point of care of patients in areas, regions, medical facilities without attention 24 / 7, or in other types of facilities where there is a low level of equipment.
  • Another advantage is that it allows the ability to identify antibiotic sensitivity at the point of patient care without the need to refer the sample to centralized laboratories.
  • micro device is applicable to the performance of antibiogram by dilution according to customer requirements.
  • the micro device is adaptable to the epidemiological conditions of each country or region
  • the micro device also complies with ISO 13.485 certification for medical devices, which facilitates registration in destination countries.
  • Figure 1 is a view of a cover with perforations for micro wells of a two-piece model of a micro device according to an embodiment of the invention.
  • Figure 2 is a view of a substrate with micro channels of a two-piece model of a micro device according to an embodiment of the invention.
  • Figure 3 is a view of a one-piece micro device according to an embodiment of the invention.
  • Figure 4 is a view of a cover with perforations for micro wells and micro channels of a two-piece model of a micro device according to an embodiment of the invention.
  • Figure 5 is a view of a substrate with perforations for micro wells of a two-piece model of a micro device according to an embodiment of the invention.
  • Figure 6 is a pair of views of a mold for manufacturing a micro device according to an embodiment of the invention.
  • Figure 7 is a pair of mask views for manufacturing a micro device according to an embodiment of the invention. DETAILED DESCRIPTION OF THE INVENTION
  • the invention describes a micro-device or medical device (10) lab-on-chip type, capable of identifying antibiotic sensitivity at the point of care of patients, especially in rural areas, offices, hospitals that have no attention 24/7, hospitals with low level of equipment, ocean liners, among others.
  • the micro devices are used in patients with urinary tract infections and the sample to be used will be urine.
  • the device (10) has a circular type preference geometry, preferably 7 to 9 cm in diameter. Said device (10) is preferably manufactured from a medical grade polymer, cyclic olefin or other material I.
  • the micro device (10) has a microcamera (20) for depositing the sample.
  • microcamera (20) From said microcamera (20) a plurality of microchannels (30) leave, where each microchannel (30) diffuses to a respective circular microwell (40), in which the process of identifying antibiotic sensitivity is developed.
  • each of the microwells (40) of each device (10) contains an antibiotic, a culture medium and a chromophore substance.
  • the antibiotics to be used correspond to those of routine use in medical practice, within which you can preferably use Pipedimic acid, Cephalotoxin, Ciprofloxacin, Cotrimozaxol, Gentamicin, Nitrofurantoin, Ampicillin, which does not exclude use of other antibiotics
  • each microchannel (30) connects the microcamera (20) with its respective microwell (40), forming a structure preferably of radial symmetry.
  • Each microchannel (30) and each microwell (40) are independent of the others, to avoid cross contamination, so that the sample that diffuses from the microcamera must have unidirectional flow.
  • capillaries preferably between 300 and 600 microns in width and about 5 mm in length, provides reasonable values of channel travel times and allows their manufacture with the available technologies.
  • a series of manufacturing processes can be used to manufacture said micro devices (10).
  • the use of 2DV2 and 3D designs stands out, as well as the manufacturing technologies for UV photolithography and chemical attack, for laser stereolithography and for casting in silicone molds, since depending on the level of detail required, the productivity required or of the materials of interest, one can resort to the use of one or the other.
  • the designs of the micro devices (10) have a central circular zone for depositing the sample corresponding to the microcamera (20) and from which radial or microchannel rails (30) arise that connect with at least seven or eight external microwells (40) for reaction.
  • Different widths and heights have been tested for lanes, from 300 to 600 microns and, with total device diameters preferably ranging from 10 mm to 40 mm, which does not limit the use of larger diameter micro devices.
  • the first embodiment includes a lid (50) and a substrate (60), wherein said lid (50) has the perforation for the microcamera (20) and perforations for the plurality of microwells (40) as shown in Figure 1.
  • the substrate (60) incorporates the microchannels (30), as shown in Figure 2.
  • the microwells (40) have greater depth for reagent encapsulation and to produce anti-return effect once each microchannel (30) has been emptied by capillarity.
  • the micro device (10) is made up of a single piece with internal microchannels (30) that connect the microcamera (20) with each microwell (40) as shown in Figure 3, to take full advantage Additive manufacturing technologies.
  • the design of the micro device (10) is oriented to an improved encapsulation of the reagents, considering the additional use of an intermediate double-sided adhesive sheet that would be perforated at the point of previous patient care. to the use of the system.
  • the micro device (10) comprises a cover (70) and a substrate (80), wherein said cover (70) comprises perforations for the microcamera (20), for the plurality of microchannels (30) and for the microwells (40) as shown in Figure 4, and said substrate (80) comprises perforations for microwells (40), as shown in Figure 5.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un microdispositif de type sur puce, permettant d'identifier la sensibilité antibiotique au centre de soins des patients, surtout dans des zones rurales, des cabinets de consultation, des hôpitaux, qui ne disposent par de soins 24/7, des hôpitaux à faible niveau d'équipement, entre autres, le microdispositif (10) ayant de préférence une géométrie de type circulaire, et comprenant une microchambre (20) dans le centre du microdispositif (10) pour déposer l'échantillon, de cette microchambre (20) sortant une pluralité de microcanaux (30), et chaque microcanal (30) diffuse l'échantillon vers un microgodet (40) respectif, dans lequel s'effectue le processus d'identification de la sensibilité antibiotique.
PCT/IB2013/059726 2013-10-28 2013-10-28 Microdispositif de type laboratoire sur puce pour identifier la sensibilité antibiotique au centre de soins des patients Ceased WO2015063540A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/IB2013/059726 WO2015063540A1 (fr) 2013-10-28 2013-10-28 Microdispositif de type laboratoire sur puce pour identifier la sensibilité antibiotique au centre de soins des patients

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2013/059726 WO2015063540A1 (fr) 2013-10-28 2013-10-28 Microdispositif de type laboratoire sur puce pour identifier la sensibilité antibiotique au centre de soins des patients

Publications (1)

Publication Number Publication Date
WO2015063540A1 true WO2015063540A1 (fr) 2015-05-07

Family

ID=53003418

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2013/059726 Ceased WO2015063540A1 (fr) 2013-10-28 2013-10-28 Microdispositif de type laboratoire sur puce pour identifier la sensibilité antibiotique au centre de soins des patients

Country Status (1)

Country Link
WO (1) WO2015063540A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002010713A2 (fr) * 2000-08-02 2002-02-07 Honeywell International Inc. Cytometre de flux portatif
US20050136685A1 (en) * 2003-12-19 2005-06-23 Kei Takenaka Chips, and apparatus and method for reaction analysis
WO2007005973A2 (fr) * 2005-07-01 2007-01-11 Honeywell International, Inc. Carte microfluidique pour analyse des hematies
WO2012011810A1 (fr) * 2010-07-22 2012-01-26 Stichting Voor De Technische Wetenschappen Dispositif de type laboratoire sur puce, par exemple pour utilisation dans l'analyse de sperme

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002010713A2 (fr) * 2000-08-02 2002-02-07 Honeywell International Inc. Cytometre de flux portatif
US20050136685A1 (en) * 2003-12-19 2005-06-23 Kei Takenaka Chips, and apparatus and method for reaction analysis
WO2007005973A2 (fr) * 2005-07-01 2007-01-11 Honeywell International, Inc. Carte microfluidique pour analyse des hematies
WO2012011810A1 (fr) * 2010-07-22 2012-01-26 Stichting Voor De Technische Wetenschappen Dispositif de type laboratoire sur puce, par exemple pour utilisation dans l'analyse de sperme

Similar Documents

Publication Publication Date Title
Sivashankar et al. A “twisted” microfluidic mixer suitable for a wide range of flow rate applications
Fu et al. Microfluidic systems applied in solid-state nanopore sensors
US9739699B2 (en) Device for the study of living cells
US20110269226A1 (en) Microfluidic Continuous Flow Device for Culturing Biological Material
Qi et al. Probing single cells using flow in microfluidic devices
US20120230886A1 (en) Microfluidic Cytochemical Staining System
US20140363838A1 (en) Microperfusion imaging platform
Natsuhara et al. A method of sequential liquid dispensing for the multiplexed genetic diagnosis of viral infections in a microfluidic device
EP2812116B1 (fr) Micropuce, dispositif contenant la micropuce et méthode de fabrication
CN206715966U (zh) 一种生物分析用微流控检测装置
Brouzes Droplet microfluidics for single-cell analysis
Zoupanou et al. SMILE platform: an innovative microfluidic approach for on-chip sample manipulation and analysis in oral cancer diagnosis
Zoupanou et al. Innovative 3D microfluidic tools for on-chip fluids and particles manipulation: From design to experimental validation
JPWO2018025705A1 (ja) 分析セル、分析デバイス、分析装置および分析システム
CN108136390A (zh) 用于执行试验的流体系统
Wang et al. A 3d-printed microfluidic device for qpcr detection of macrolide-resistant mutations of mycoplasma pneumoniae
CN102500266B (zh) 一种用于高粘度溶液的快速微混合装置
Wang et al. An integrated centrifugal degassed PDMS-based microfluidic device for serial dilution
Kuo et al. A spatiotemporally defined in vitro microenvironment for controllable signal delivery and drug screening
JP2016514965A (ja) 試料中の細胞を分類する装置及び該装置の使用方法
Wang et al. A compact, syringe-assisted, vacuum-driven micropumping device
WO2015063540A1 (fr) Microdispositif de type laboratoire sur puce pour identifier la sensibilité antibiotique au centre de soins des patients
Walsh III et al. Emulation of colonic oxygen gradients in a microdevice
Sukthang et al. Sensitivity validation of EWOD devices for diagnosis of early mortality syndrome (EMS) in shrimp using colorimetric LAMP–XO technique
CN212833791U (zh) 一种细胞功能检测的微流控芯片

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13896673

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 16107928

Country of ref document: CO

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 13896673

Country of ref document: EP

Kind code of ref document: A1