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WO2015041556A1 - Procédé et utilisation d'additifs dans la conservation d'aliments pour animaux - Google Patents

Procédé et utilisation d'additifs dans la conservation d'aliments pour animaux Download PDF

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Publication number
WO2015041556A1
WO2015041556A1 PCT/RS2014/000008 RS2014000008W WO2015041556A1 WO 2015041556 A1 WO2015041556 A1 WO 2015041556A1 RS 2014000008 W RS2014000008 W RS 2014000008W WO 2015041556 A1 WO2015041556 A1 WO 2015041556A1
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Prior art keywords
silage
carbon dioxide
dry ice
silos
plants
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PCT/RS2014/000008
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English (en)
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Aleksandra IVETIĆ
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B7/00—Preservation of fruit or vegetables; Chemical ripening of fruit or vegetables
    • A23B7/14—Preserving or ripening with chemicals not covered by group A23B7/08 or A23B7/10
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01F—PROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
    • A01F25/00—Storing agricultural or horticultural produce; Hanging-up harvested fruit
    • A01F25/16—Arrangements in forage silos
    • A01F25/166—Arrangements in forage silos in trench silos
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
    • A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging

Definitions

  • the present invention relates to the use of carbon dioxide, in two different aggregate states, that is, the application of carbon dioxide in solid form (dry ice) and in gaseous state, in the process of conserving / ensiling feed.
  • the problem being solved by present invention is to provide, in a short period of time, anaerobic conditions for the growth and development of LAB (lactic acid bacteria) and lactic acid fermentation in silage with reduced loss of nutrients, as well as adaptation to the conditions in each farm individually depending on its capabilities in a completely new and safe way with regard to human and animal health, as well as on the protection of the environment.
  • LAB lactic acid bacteria
  • additives is intended to create the final product of fermentation, especially lactic acid which is used as a preservative of silage, and then the acetic acid which has a useful role in the duration of the aerobic stability (Filia I. et al., 2007, Journal of Diary Sci. 90 : 5108-5114).
  • Lactic acid has a weak fungicidal properties, in contrast to acetic and propionic acid. Therefore, the microbiological composition of the additives is different, i.e. that may be inoculants heterofermentative or homofermentative LAB, or a combination thereof.
  • inoculants of heterotrophic LAB are added to the green mass to ensile, aerobic stability of the silage is improved in addition to the production of lactic and of acetic acid (Moon NJ, 1983, J. Appl. Bacteriol. 55: 453-460), whereas with specific microbial inoculants, such as L.Buchneri, the concentration of acetic acid is increased (Hu et al, 2009, J. Diary Sci. 92: 3907-3914).
  • Patents which describe the use of biological additives in the form of inoculants of LAB are : CN101940272 which describes the use of probiotics, JP200805833 describes adding yogurt as a scrap of food industry, CN103060228, CN102994423, CN102851233 relating to the use of homofermentative LAB, and different strains of Lactobacillus plantarum ; WO2008073853, WO2008073848, WO2008073844, WO2008073841 , WO2008073839, WO2008073839, WO2008073835 regarding the use heterofermentative LAB, and different strains of Lactobacillus Buchneri; AT507290, WO2013001862, KR20120126917, US201 1 142991 , NZ591040 relating to the use of a combination of homo-and heterofermentative LAB.
  • Patents which describe the use of chemical additives are: DE102007008804 describes a combination of various acids, JP2008199943 which discloses the use of amino acids, while the patent US2008317934 describes the use of propionic acid, and WO2013056829 and WO201 1050478 describe the use of enzymes.
  • Patent CN103053808 and CN10305161 relating to the use of an extract from different plant species, whereas in patent UA28261 straw is added, and in the patent GR1007162 molasses is used in the preparation of the silage. Clime is one of the factors that affect the process of ensiling.
  • the increase in temperature of the environment leads to additional heating of the silo mass, which is one of the risks where the transformation of the present sugar and heat generation, resulting in an increase in temperature (Kunkle et al, 2006, UF / The Inst. Of Food and Agric. Sci. (IFAS), SS-AGR- 177). If the temperature reaches 50 ° C during aerobic activity Maillard reaction can occur, which reduces the digestibility of proteins in silo mass (Muck et al, 2003, Silage Science and Technology, Inc., Madison, Wl, USA, p. 251-304). Also, exposure silo mass to the air extends the activity of harmful microorganisms, such as yeasts and molds, and thus the development of the LAB is disposed, which leads to a deterioration in the quality of silage.
  • harmful microorganisms such as yeasts and molds
  • WO 2000045645 describes ensiling in silage bales with a high content of SM over 60% and the use of liquid injector for introducing C0 2 into bales.
  • the main disadvantage of the WO 2000045645 patent is uneconomical. Specifically, it is necessary to separate more funds for the purchase of additional equipment and special machinery for harvesting plants, while in the present invention used existing silo facilities on the farm, with no additional investment. Further, in the aforementioned patent, have been used commercially available additives, without specifying the origin, which represents disadvantage because it can not be concluded certain advantages. Also, the difference is in the fact that it uses liquid C0 2 . All this is from an economic point of view, one major drawback and difference, which is solved by the present invention, because represents ease application and the farmer does not invest in additional equipment, i.e. in its current silage production should not change anything.
  • WO 9418847 uses blocks of 5 kg and 1 kg, whereas in the present invention were used pellets of a diameter of 16 mm and a length of 8 cm, in order to be as small as possible a layer of dry ice, between layers of the plants, because it avoids the formation of air pockets during the sublimation. Also, the amount of dry ice used is 1-6 kg / 1 of plants, while in the present invention uses the percentage of amount which is calculated according to the dimension of the object, the amount of ensiled plants, feeding plants value and its chemical parameters and what is most important is that the application tailored to the specific conditions at each farm and its possibilities. But the main disadvantage of the above prior patent WO 9418847 is used inoculant that masks the independent effect of C0 2 .
  • inoculants does not exclude the initial phase of respiration where oxygen is still present and trapped between plant parts and to work undesirable microorganisms that consume nutrients and oxygen use. As long as all the oxygen is not consumed by undesirable microorganisms, they work, grow and develop in the silo mass, degrading its quality and what is left of nutrients after them to use inoculants, and at this stage it is idle because it is waiting to anaerobic conditions. Based on the above, we see that he does not teach nor suggests the application only C0 2 in solid or gaseous state.
  • the present invention relates to the preservation of animal feed and presents an inventive element in the process of ensiling plants. It makes a big difference in the appearance of the commercial application of additives and is an important finding, which essence is reflected in the ease and simple application, effectiveness and safety.
  • the novelty of the invention and application of the new technology is the use of C0 2 in different aggregate states during the ensiling, acts immediately, there is no waiting for the completion phase of aerobic respiration, because in the only 0-2 hours provide anaerobic conditions for growth and development of lactic acid bacteria that are present in the epiphytic micro flora of the plants (on the surface of green plants).
  • Silage has an excellent quality class after 8 and 18 days of opening of the silo, and the silo opening with a routine of the 40th day. This is particularly useful on a farm where, in the case of deficit of feed, preferably silos has to be open earlier than 40 days, which has so far not been possible because of the time needed to conduct quality lactic acid fermentation and stabilization the processes of conservation.
  • Figure 1 - graphical presentation of addition the dry ice in three layers, each layer in one third of silos during the filling with plants
  • Figure 2 - graphical presentation of addition the dry ice in a single layer on the lower third of silos during the filling with plants
  • Figure 3 - graphical presentation of addition the dry ice in a one layer on the lower and the upper third of silos during the filling with plants
  • Figure 4 -graphical presentation of addition the dry ice in a single layer on the upper third of silos during the filling with plants
  • Figure 5 - graphical presentation of addition the dry ice though a perforated tube placed vertically, at the final stage of filling the silos with plants
  • Figure 6 graphical presentation of gas C0 2 insertion through two perforated tubes placed horizontally, at the final stage of filling the silos with plants
  • Preserving feed for feeding domestic animals is used in agriculture around the world. Plants after harvest or mowing are transported from the field to the farm, inserted into the silo, compressed to a displacing the air, and then with the special foils covers the silos order to create the conditions for fermentation progress.
  • Silage is created by the conservation of plants with spontaneous lactic acid fermentation. Preservation of plants is done with the acid that is made with activity of LAB during the spontaneous lactic acid fermentation. The basis for successful conservation of plants in the form of silage is an anaerobic environment. This condition is required for activity of LAB, because they produce lactic acid in the course of their metabolism.
  • the produced a sufficient amount of lactic acid is used as the preservative, lowers the pH of the initial value in green plant and helps to maintain the nutritional value of silage plants. If oxygen is present in the middle of silo mass, it favors the development of harmful aerobic microorganisms such as molds and yeasts which cause spoilage and deterioration quality in process of ensiling, due to such animal feeding with bad silage quality results in poisoning of animals.
  • Aerobic phase starts immediately after harvest and continues until the oxygen is present in ensiled plants.
  • this initial phase which is also called the phase of respiration plants
  • herbal sugar in freshly cut material are decomposed by heat creating.
  • Aerobic microorganisms yeasts, molds and aerobic bacteria
  • yeasts, molds and aerobic bacteria are present in the chopped plant material and also use the sugars in the initial phase of respiration.
  • Progressive and rapid development of yeasts and molds leading to warming of silo mass, which has a negative consequence on the end of the process, at the opening of the silo and animal nutrition, and result, is the poor quality silage for animal feed.
  • Phases of chemical changes during ensiling plants are: 1) respiration of plant material, 2) the creation of acetic acid, 3) the creation of lactic acid, 4) settling the fermentation process, 5) if the ensiling process was not properly implemented - the creation of butyric acid and 6) aerobic stability.
  • the first phase of respiration begins immediately because oxygen is present, which allows the growth and development of various MO (microorganisms) that are present on the plant and represent competitors to a LAB, using for their metabolism nutrients that is necessary to preserve during conservation.
  • MO microorganisms
  • the most tumultuous changes are happened (which it cannot quite squeeze with traditionally technology of ensiling), so that oxidative processes are enabled.
  • Consumption of air which was trapped in the silo mass during the initial respiratory is the trigger that activates the beginning of anaerobic fermentation phase and is the first phase of the production of acetic acid and immediately after the lactic acid and respectively lactic acid fermentation phase begins.
  • Enterobacteria which are tolerant to an increase in the heat (in the aerobic phase), producing a number of different products.
  • These bacteria ferment carbohydrate soluble in water (WSC) and hexoses (glucose and fructose), producing: Volatile fatty acids (VFA ' s) a short carbon chain - acetic, lactic and propionic, ethanol and C0 2 .
  • WSC carbohydrate soluble in water
  • VFA ' s Volatile fatty acids
  • Heterotrophic bacteria of the second phase are insufficient fermenters because they produce very little preserving acid in exchange for the loss of nutrients.
  • the ratios of the creation of acetic acid, in this stage depend on the maturity of the plant, content of moisture and the epiphytic population of bacteria.
  • Enterobacteria are sustainable at pH 5-7, and their work formed VFA ' s that reduce the pH value below pH 6. Therefore, heterofermentative bacteria are inhibited with acids they produced. Enterobacteria create a path to a LAB, which is sustainable at a pH below 5. The drop of pH value indicates the end of the initial anaerobic phase, which typically lasts for 24-72 hours.
  • Phase of lactic acid fermentation is the largest and the most important for the preparation high quality silage and preserving the nutritional value of ensiled plant.
  • major and necessary product of microbial activity is lactic acid, which is a preservative in silage.
  • Lactic acid is dominant in silages best quality (more than 60% of the total VFA ' s), and present is at the level of 3-6% dry matter (DM) in silage with good quality.
  • LAB usually containing strain Lactobacillus plantarum
  • the fermentation process are faster, with a more conserved WSC and nutrients, peptides and amino acids.
  • the duration of this phase is from 8 days to 3 weeks.
  • Such a large time ratio depend on the buffer capacity of the plants, the moisture content and the maturity of the crop which is to ensile.
  • LAB can be divided into four main groups: lactobacilli, streptococci, pediococci and leuconostoc. Growth of Lactobacillus in the substrate is often improved by the anaerobic environment comprising 5-10% C0 2 . LAB can tolerate a temperature range of their growth between 2-53°C and most optimum is 30-40°C, are more sensitive to high temperatures than lower in areas where growth. For the isolation of strains of LAB, liquid glycerol is used at a temperature of -80°C and the temperature of the dry ice -79°C, with no side effect on their potency. The optimum pH value for its growth is in the range from pH 6.2 to 5.5 to the lower value. LAB species important for silage
  • A. Homofermentative lactobacilli Lb. casei, Lactobacillus coryniformis, Lb. curvatus, Lactobacillus plantarum
  • Homofermentative LAB ferments one mole of hexoses (glucose and fructose) and provides two moles of lactic acid, while fermentation of pentose provides one mole of lactic acid, and one mole of acetic acid.
  • Heterofermentative LAB are less efficient in terms of conversion of carbohydrate, fermentation of 1 mole of glucose provides one mol of lactic acid, acetic acid (a part in the aerobic stability), or ethyl alcohol and carbon dioxide.
  • Fermentation phase of settling (stable phase) occurs when lactic acid its reached maximum in the silage crop mass, and caused the lowering of pH below 4.2. Further development and biochemical activity of lactic acid bacteria is slowed and almost development of the others anaerobic bacteria completely stops. Aerobic microorganisms cannot develop due to lack of oxygen, which is partially depleted by the respiration of plant tissues, and partly dissipated with development of aerobic and facultative anaerobic microorganisms, in the first and second stage of ensiling. The first two phases have short duration, one to two days, if are applied correct technique of ensiling. The third and fourth phases are longer, 15-20 days, which makes the process of fermentation completed in about three weeks.
  • Stable phase lasts during storage. This phase is not static due to various processes that can take place, depending on environmental conditions such as air penetration or the number and type of MO present on epiphytic microflora in the plants before ensiling. The amount of fermented substrate stays at the same level and type of fermentation acids produced in the silage.
  • Silage with Clostridia (CI. Botulinum) has a higher content of butyric acid, which is a preservative for inhibiting the activity of yeasts and molds, but is not desirable. If the process of conserving silage fails completely and the pH is above 4.6 to 4.7, may be further developed microbial transformation and degradation.
  • a typical " Clostridia silage" has a higher content of butyric acid (more than 5 g / kg DM), high pH (over 5) and a high content of ammonia and amines.
  • Clostridia are most sensitive to the low value of water activity (a w ) in contrast to the LAB.
  • Phase of aerobic quality degradation of silage starts immediately when the silos are opened for animals feeding and silage is exposed to air. During the feeding of animals, this stage is inevitable and occurs in all silage regardless of its quality. It consists of two stages. The first is the beginning of the deterioration due to the degradation of protective organic acids. Increasing the pH begins the second phase of decay in which the temperature increases and the speed of growth of microorganisms. Aerobic degradation occurs in the all quality types of silages, which are open and exposed to air. Air (oxygen) is a major cause of deterioration of silage, because it makes possible unwanted chemical and microbiological activity, which leads to deterioration of silage.
  • Air oxygen
  • the air trapped in the silage, as well as the penetration of air into the silo and silage air exposure during feeding are responsible factors that lead to aerobic deterioration of silage.
  • Up to 50% loss of dry matter (DM) is caused by aerobic degradation on the surface of silage.
  • Some indicators of aerobic degradation of silage can be seen during a visit to the farms. For example, color silage smell (moldy smell or not), visible fungus (possibly yeast), a sense of high temperature, wet silage as a result of aerobic instability of some of the parameters that can be easily seen on farms.
  • the essence of the present invention which provides the anaerobic conditions, is the addition of carbon dioxide (C0 2 ), in solid (dry ice) or gaseous aggregate state during ensiling feed, during the filling the silos with green plants in silos, compacting and covering the same silos.
  • C0 2 carbon dioxide
  • dry ice solid
  • gaseous aggregate state during the filling the silos with green plants in silos, compacting and covering the same silos.
  • Invention procedure with direct insertion of C0 2 acts immediately to silo weight, because there is no waiting time for growth of LAB and phase of aerobic respiration, but in the course of only 0-2 hours to ensure anaerobic conditions.
  • Invention provides 50 times faster formation the anaerobic conditions in the silo, compared to the traditional technology of ensiling, where anaerobic phase occurs in the silage mass plant for 100 hours on average in good condition, and this fact highlights the difference and present invention advantage, in the time provided for growth and development LAB and securing desirable lactic acid fermentation.
  • C0 2 from the ecological aspect is especially important because it is a harmless material, as well as all its forms created by dissolving, natural ingredients and as such does not change the quality of animal feed.
  • C0 2 gas is a colorless, odorless and tasteless, does not burn or supports combustion, is present in the atmosphere. Created by the combustion of hydrocarbons and occurs in three states of matter, gaseous, liquid and solid. Solid C0 2 is in the form of dry ice, the freezing point is -79 0 C. C0 2 is obtained by processing natural gas and industrial waste gas, so its price in the market is very low. Dry ice is industrially produced by separating, purifying and compressing C0 2 from waste gases or from natural gas.
  • the influence of C0 2 in solid or gaseous was investigate on the quality of maize silage hybrids PR32D12 (H1) and PR33T56 (H2) which are prepared separately in separate silos with the addition of C0 2 in solid (dry ice), 100 g per silo, with a different type of treatment, with direct or indirect contact with the silage mass of C0 2 .
  • the influence of C0 2 in gaseous state was investigate on the quality of maize silage hybrid PR33T56 (H2) which are prepared in separate silos with the insertion gas C0 2 .
  • silage silos were opened (with dry ice and with gas C0 2 ) and analyzed the chemical parameters on the quality, i.e. determine the content of dry matter (DM), crude ash, crude protein (CP), crude fat (EE), insoluble fiber content of neutral detergent (NDF) and insoluble fiber in acid detergent (ADF), lignin (ADL) and is also determined the content of lactic, acetic and butyric acid.
  • DM dry matter
  • CP crude protein
  • EE crude fat
  • NDF neutral detergent
  • ADF insoluble fiber in acid detergent
  • ADL lignin
  • Maize hybrid is also investigated for gas insertion during ensiling.
  • Silage is stored in a temperature of 26 ⁇ 2 ° C, in order to avoid the influence of the outside temperature oscillations.
  • pellets are used in their original form, with a diameter of 16 mm and length of 8cm, because it is unnecessary to cutting.
  • all silos tanks were exposed to all the temperature changes of the environment, and opened the 8, 18, 40 and 100 days per charge and coverage.
  • One embodiment of the present invention represents direct addition of dry ice in the ensiled green plants, in this case corn hybrid H1 , which is ensiled in 12 separate anaerobic silos (4 treatments in triplicate, 12 per hybrid), volume 1.5 I.
  • Treatments for H1 were: control (no dry ice), adding 3 layers of dry ice (33.33 g in each layer charge). Silos were open at 8 and 18 days, after silos covering. The results of this method are shown in Table 1.
  • Another embodiment of the present invention is influence of direct addition of dry ice in the ensiled feed, in this case corn hybrid H2, which is ensiled in a separate 12 (four treatments in triplicate, 12 per each hybrid) anaerobic silos, volume 1.51, for 40 days .
  • Treatments for H2 were: control (no dry ice), adding layers of dry ice (33.33 g in each layer charge) three layers, dry ice at the bottom of the jar (100 g) one layer, the addition of dry ice in the bottom and upper third (50 g in each layer of filling) two layers .
  • Table 2 The results of those methods are shown in Table 2.
  • Table 2 Chemical parameters of nutritive value of corn silage hybrid PR 33T56 (H2) treated with dry ice and the opening of the silo 40th day
  • Further embodiment of the present invention relates to testing the difference between the application of the C0 2 , in a solid and gaseous state, where the analysis of inserted gas C0 2 into the silo plant mass was performed. It was used 9 anaerobic silos, volume of 1.5 I (three treatments in triplicate, 9 per hybrid).
  • C0 2 gas is inserted through a perforated tube which is placed horizontally.
  • silos dry ice was added through perforated tube placed into green mass before next plant filling and closing the silos.
  • dry ice was added directly (100 g) in the last third at the top, in one layer, before the closing the silo. The results of those methods are shown in Table 3.
  • Table 3 Chemical parameters of nutritive value of corn silage hybrid PR 33T56 (H2) treated with dry ice and insertion of C0 2 gas, with the silo opening at the 40th day
  • the present invention decreases the aerobic phase due to sublimation of the solid C0 2 (dry ice) or in the form of gas C0 2 and displaces the air, allowing the formation of anaerobic fermentation, according to the diagram, we can see that the aerobic phase lasts only 0-2 hours, less than a day, and then immediately begins fermentation phase accompanied by anaerobic conditions (indicated by the start arrow), where the curve of LAB growing is exponentially, while the curve pH value decreases exponentially during the first days, which means quick action of C0 2 and its effectiveness in the occurrence of the fermentation stage during the first day.
  • Analyzed parameters of estimated nutritional value indicate preservation of the nutritional value of the plants during fermentation.
  • Content of ash was not increased compared to the initial content in the fresh plants before ensiling, suggesting that the losses left behind, and all this accompanied by content of DM, with fluctuations of only 0.5%.
  • the contents of crude protein and crude fat were increased in relation to the content in the fresh plant, compared in this case fresh maize plant before ensiling in an oscillation of 0.5-1 % by varying the mode of application of C0 2 in solid or gaseous.
  • the results of the fiber content indicate that there is a degradation and splitting the cell wall of plants in the treated maize silage, because the lignin content in the treated silage decreased by an average of 40% compared to fresh plant before ensiling, which directly reflects increasing its nutritional value, because the indigestible part of the of the plant is lignin.
  • the application of the present invention increase the preservation and utilization of nutrients and digestible energy of ensiled plants, and thus the present invention can be used in silage which requires opening the silo after only 8 days of ensiling, as is the case in the absence of feed on the farms.
  • silage treated with dry ice or the gas C0 2 has an extremely longer duration of aerobic stability which protects them from degradation in the quality after the silos are opened and exposed to air, which is a great advantage of the present invention.
  • the present invention is adapted to the specific circumstances on the farm, as well as to the conditions of the outside temperature.
  • the method of application C0 2 in the gaseous state is the more appropriate.
  • recommend method is the addition of C0 2 in the solid state in three layers, due to higher lignin content.
  • the recommended method of the present innovation is application of C0 2 in the solid state (dry ice), 0.04% of the volume of entrapped air in a one layer in the lower third or in the upper third layer during the filling of the silos with fresh plants.
  • the applied methods of the present inventions will depend also on the manner and mode of covering the silos on the farms.

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Abstract

La présente invention concerne l'utilisation de dioxyde de carbone, dans deux différents états d'agrégat, c'est-à-dire l'utilisation de dioxyde de carbone à l'état solide (glace sèche), ou à l'état gazeux dans le procédé d'ensilage/conservation d'aliments pour animaux.
PCT/RS2014/000008 2013-09-23 2014-09-17 Procédé et utilisation d'additifs dans la conservation d'aliments pour animaux Ceased WO2015041556A1 (fr)

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RS20130405A RS55497B1 (sr) 2013-09-23 2013-09-23 Postupak i primena aditiva pri konzervisanju hrane
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020092728A1 (fr) * 2018-11-01 2020-05-07 The Gombos Company, LLC Composition d'un produit d'alimentation du bétail et son procédé de fabrication
EP3892086A1 (fr) * 2020-04-07 2021-10-13 Deere & Company Procédé et agencement de production d'ensilage
WO2022045914A1 (fr) 2020-08-31 2022-03-03 University Of Belgrade Stabilisants d'ensilage

Citations (28)

* Cited by examiner, † Cited by third party
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US2952541A (en) * 1958-09-29 1960-09-13 Fayhee Paul Edwin Crop preserving structures and methods
JPS5716661A (en) * 1980-06-30 1982-01-28 Hoxan Corp Method for keeping silage quality using carbon dioxide gas
WO1994018847A1 (fr) 1993-02-24 1994-09-01 Bernd Pieper Procede pour l'ensilage du fourrage
WO2000045645A1 (fr) 1999-02-04 2000-08-10 Ensigas As Procede d'ensilage de fourrage
UA28261U (en) 2007-10-12 2007-11-26 Method for ensilaging feeds
JP2008005833A (ja) 2006-05-29 2008-01-17 Masaaki Tsugeta サイレージ及びその調製方法
WO2008073839A2 (fr) 2006-12-11 2008-06-19 Pioneer Hi-Bred International, Inc. Souche ln1286 de lactobacillus buchneri et son utilisation permettant d'améliorer la stabilité aérobie d'un ensilage
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US11723385B2 (en) 2018-11-01 2023-08-15 The Gombos Company, LLC Composition of livestock feed and method of manufacturing the same
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WO2022045914A1 (fr) 2020-08-31 2022-03-03 University Of Belgrade Stabilisants d'ensilage

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