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WO2014205595A1 - Atp hydrolase atpase-1 dérivée du coton, gène codant et utilisation de ceux-ci - Google Patents

Atp hydrolase atpase-1 dérivée du coton, gène codant et utilisation de ceux-ci Download PDF

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Publication number
WO2014205595A1
WO2014205595A1 PCT/CN2013/000748 CN2013000748W WO2014205595A1 WO 2014205595 A1 WO2014205595 A1 WO 2014205595A1 CN 2013000748 W CN2013000748 W CN 2013000748W WO 2014205595 A1 WO2014205595 A1 WO 2014205595A1
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WO
WIPO (PCT)
Prior art keywords
plant
seq
gene
expression vector
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2013/000748
Other languages
English (en)
Chinese (zh)
Inventor
王建胜
何云蔚
梁远金
陈淼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BIOCENTURY TRANSGENE (CHINA) Co Ltd
Original Assignee
BIOCENTURY TRANSGENE (CHINA) Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BIOCENTURY TRANSGENE (CHINA) Co Ltd filed Critical BIOCENTURY TRANSGENE (CHINA) Co Ltd
Priority to PCT/CN2013/000748 priority Critical patent/WO2014205595A1/fr
Priority to CN201380074512.6A priority patent/CN105073984B/zh
Publication of WO2014205595A1 publication Critical patent/WO2014205595A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

Definitions

  • African cotton (National Cotton Medium Term Bank, obtained by the China Cotton Research Institute, Uniform No.: ZM-06838) Seeded on sterilized vermiculite, cultured at 25 °C, light dark cycle 16h/8h, poured 1 per week /2MS medium ( 9. 39 mM KN0 3 , 0. 625 mM KH2P0 4 , 10. 3 mM NH 4 N0 3 , 0.75 mM MgS0 4 , 1. 5 mM CaCl 2 , 50 ⁇ M KI , 100 ⁇ MH 3 B0 3 , 100 ⁇ M MnS0 4 , 30 ⁇ M ZnS0 4 , 1 ⁇ M Na 2 Mo0 4 , 0. 1 ⁇ M CoCl 2 , 100 ⁇ M Na 2 EDTA, 100 ⁇ M FeS0 4 ) once. It was used for experiments when the seedlings were as long as 25-30 cm.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne une protéine végétale, un gène codant et une utilisation de ceux-ci, en particulier, une ATP hydrolase dérivée du coton, un gène codant de celle-ci, et l'utilisation de ceux-ci dans la culture d'une plante transgénique présentant une tolérance accrue au sel.
PCT/CN2013/000748 2013-06-24 2013-06-24 Atp hydrolase atpase-1 dérivée du coton, gène codant et utilisation de ceux-ci Ceased WO2014205595A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/CN2013/000748 WO2014205595A1 (fr) 2013-06-24 2013-06-24 Atp hydrolase atpase-1 dérivée du coton, gène codant et utilisation de ceux-ci
CN201380074512.6A CN105073984B (zh) 2013-06-24 2013-06-24 一种棉花ATP水解酶ATPase‑1及其编码基因与应用

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2013/000748 WO2014205595A1 (fr) 2013-06-24 2013-06-24 Atp hydrolase atpase-1 dérivée du coton, gène codant et utilisation de ceux-ci

Publications (1)

Publication Number Publication Date
WO2014205595A1 true WO2014205595A1 (fr) 2014-12-31

Family

ID=52140731

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2013/000748 Ceased WO2014205595A1 (fr) 2013-06-24 2013-06-24 Atp hydrolase atpase-1 dérivée du coton, gène codant et utilisation de ceux-ci

Country Status (2)

Country Link
CN (1) CN105073984B (fr)
WO (1) WO2014205595A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113151351A (zh) * 2021-03-29 2021-07-23 西南大学 一种提高棉花种子质量和油脂含量的方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118792343B (zh) * 2024-07-23 2025-07-25 西南林业大学 一种木葡聚糖内转糖苷酶/水解酶基因的应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007166921A (ja) * 2005-12-19 2007-07-05 Chugoku Electric Power Co Inc:The Ｈ＋−ａｔｐアーゼ遺伝子
JP2008259475A (ja) * 2007-04-13 2008-10-30 Chugoku Electric Power Co Inc:The Ｈ＋−ａｔｐアーゼ遺伝子
CN102952816A (zh) * 2011-08-23 2013-03-06 中国科学院遗传与发育生物学研究所 ATPase蛋白在植物耐逆性中的应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007166921A (ja) * 2005-12-19 2007-07-05 Chugoku Electric Power Co Inc:The Ｈ＋−ａｔｐアーゼ遺伝子
JP2008259475A (ja) * 2007-04-13 2008-10-30 Chugoku Electric Power Co Inc:The Ｈ＋−ａｔｐアーゼ遺伝子
CN102952816A (zh) * 2011-08-23 2013-03-06 中国科学院遗传与发育生物学研究所 ATPase蛋白在植物耐逆性中的应用

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 7 December 2011 (2011-12-07), "calcium-transporting ATPase 12, plasma membrane-type [ Vitis vinifera (wine grape) ]", accession no. P_002274787 *
TUSKAN, G.A. ET AL.: "The genome of black cottonwood, Populus trichocarpa (Torr. & Gray", SCIENCE, vol. 313, no. 5793, 15 September 2006 (2006-09-15), pages 1596 - 1604 *
XIAO, ZHONGYI ET AL.: "Overexpression of cotton, vacuolar H+-ATPase subunit C promotes cell elongation and regulates stress tolerance in fission yeast", HEREDITAS, vol. 30, no. 4, April 2008 (2008-04-01), pages 495 - 500 *
XIAO, ZHONGYI: "Cloning and expression analysis of GhDET3, a vacuolar H+-ATPase subunit C gene , from cotton", JOURNAL OF GENETIC AND GENOMICS, no. 35, 26 January 2008 (2008-01-26), pages 307 - 312 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113151351A (zh) * 2021-03-29 2021-07-23 西南大学 一种提高棉花种子质量和油脂含量的方法

Also Published As

Publication number Publication date
CN105073984B (zh) 2017-11-10
CN105073984A (zh) 2015-11-18

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