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WO2014200380A1 - Préparation antiseptique, et procédés de production et d'utilisation - Google Patents

Préparation antiseptique, et procédés de production et d'utilisation Download PDF

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Publication number
WO2014200380A1
WO2014200380A1 PCT/RU2013/000477 RU2013000477W WO2014200380A1 WO 2014200380 A1 WO2014200380 A1 WO 2014200380A1 RU 2013000477 W RU2013000477 W RU 2013000477W WO 2014200380 A1 WO2014200380 A1 WO 2014200380A1
Authority
WO
WIPO (PCT)
Prior art keywords
type
antiseptic preparation
cocoalkyl
silver
antiseptic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/RU2013/000477
Other languages
English (en)
Russian (ru)
Inventor
Альберт Николаевич ДЕНИСОВ
Юрий Андреевич КРУТЯКОВ
Алексей Александрович КУДРИНСКИЙ
Павел Михайлович ЖЕРЕБИН
Сергей Николаевич ЕЛАНСКИЙ
Марина Александровна ПОБЕДИНСКАЯ
Алексей Игоревич КЛИМОВ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
OBSCHETSVO S OGRANICHENNOY OTVETSTVENNOSTYU "NANOBIOTEKH"
Original Assignee
OBSCHETSVO S OGRANICHENNOY OTVETSTVENNOSTYU "NANOBIOTEKH"
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by OBSCHETSVO S OGRANICHENNOY OTVETSTVENNOSTYU "NANOBIOTEKH" filed Critical OBSCHETSVO S OGRANICHENNOY OTVETSTVENNOSTYU "NANOBIOTEKH"
Priority to PCT/RU2013/000477 priority Critical patent/WO2014200380A1/fr
Publication of WO2014200380A1 publication Critical patent/WO2014200380A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/38—Silver; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/02—Local antiseptics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery

Definitions

  • the invention relates to broad-spectrum antiseptic preparations and can be used in agriculture, veterinary medicine, medicine, cosmetology, for disinfection in industry and at home, including for the disinfection of water, premises, equipment and inventory.
  • the claimed antiseptic drug can be used as a fungicide in growing plants.
  • Cationic surfactants based on quaternary ammonium bases are used in medicine as antiseptic and disinfectants (for example, RF patents N ° 2161961 dated January 20, 2001, JV92 I 64135 dated March 20, 2001, JV ° 22343 13 dated 08.20.2004).
  • the disadvantage of these drugs is the need to use sufficiently concentrated solutions to achieve a disinfecting effect, for example, up to 0, 1 wt.% For miramistin (RF patent N ° 2164135 of 03.20.2001).
  • Antibacterial activity is also shown by colloidal silver preparations.
  • the disadvantage of these preparations is the need to use sufficiently concentrated colloidal silver solutions to achieve a disinfecting effect, for example, from 0.1 mass% to 0.4 mass% for the Argovit preparation (RF patent N ° 2342120 dated December 27, 2008 ) in terms of the silver content in the diluted preparation.
  • an antibacterial composition based on colloidal silver stabilized with citric acid or its salts suspended in water is disclosed, additionally containing at least one cationic surfactant.
  • a disinfectant for treating skin is disclosed containing colloidal silver, at least one cationic surfactant, auxiliary additives and water in the following ratio of components, wt.%: Colloidal silver 10 "5 - 0.5; cationic surfactant 0.01 -25; water and auxiliary additives up to 100.
  • compositions including cationic surfactants and colloidal silver One of the possible reasons for the appearance of increased antibacterial activity in compositions including cationic surfactants and colloidal silver is that positively charged silver particles coated with cationic surfactants bind well to the negatively charged bacterial cell membrane.
  • a common disadvantage of preparations based on silver nanoparticles stabilized by cationic surfactants is the insufficient resistance of such silver sols to aggregation in an alkaline medium and at high concentrations of electrolytes.
  • a dispersion of silver nanoparticles stabilized by a cationic surfactant with benzyl dimethyl- [3- (myristoylamino) propyl] -ammonium chloride loses its resistance to aggregation at a potassium chloride concentration of 0.24 mol / L.
  • the task arises of increasing the effectiveness of antibacterial drugs based on surfactants and colloidal silver, in particular, increasing their stability.
  • the invention disclosed in the patent of the Russian Federation L 2419439 dated 05/27/2011 1 year is the closest analogue of the claimed invention.
  • amphoteric surfactants exhibit pronounced biocidal activity against many prokaryotic and eukaryotic microorganisms, including gram-positive and gram-negative bacteria, fungi.
  • silver nanoparticles stabilized by amphoteric surfactants are stable over a wide pH range and are resistant to aggregation in the presence of electrolytes, which makes it possible to use dispersions of silver nanoparticles stabilized by amphoteric surfactants as broad-spectrum antiseptic drugs, including fungicides when growing plants.
  • biocidal activity with respect to the microorganisms used for testing from all obtained dispersions of silver nanoparticles stabilized by amphoteric surfactants is most pronounced for dispersions containing derivatives of ⁇ -aminocarboxylic acids and iminodicarboxylic acids, for example, ⁇ -alkyl substituted derivatives of aminoacetic acid, 3-aminopropionic acid, acid and iminodipropionic acid.
  • other dispersions for example, containing amphoteric surfactants belonging to the class of ⁇ -alkyl betaines, also exhibit biocidal activity against the used microorganisms. This allows us to generalize the results of experimental studies and conclude that it is possible to use dispersions of silver nanoparticles stabilized by amphoteric surfactants as antiseptic preparations.
  • the claimed invention relates to an antiseptic preparation for the disinfection of water, premises, equipment and inventory in industry and at home, for the antiseptic treatment of intact and damaged skin and mucous membranes, for the treatment of plants, seeds and fruits.
  • the claimed invention relates to an antiseptic preparation, in particular to a fungicide comprising nanosized particles of silver and at least one amphoteric surfactant.
  • the concentration of amphoteric surfactant in the antiseptic preparation is from 0.001 wt.% To 20 wt.%.
  • the concentration of nanosized silver particles in the antiseptic preparation is from 10 "4 wt.% To 0.5 wt.%.
  • at least one amphoteric surfactant is selected from the group consisting of carboxylic acids and their derivatives with the general formula
  • substituents M i and M 2 are selected from the group consisting of H, Na, K, NH 4 , wherein the number a is 1 or 2, the number b is 2 or 3, the number c is I or 2, the number m is 1 or 2, the number n is 0 or greater than 0, the number p is 0 or greater than 0, the number q is greater than 0, and the substituent R] is selected from the group consisting of branched and unbranched saturated and unsaturated linear and cyclic hydrocarbon radicals.
  • At least one amphoteric surfactant is selected from the group consisting of M- (2-ethylhexyl) iminodipropionic acid and its salts, ⁇ -octyliminodipropionic acid and its salts, ⁇ -thalloalkyliminodipropionic acid and its salts, ⁇ -cocoalkyliminodipropionic acid and its salts, ⁇ -cocoalkylaminopropionic acid and its salts, a type I compound in which R
  • Mi and M 2 are Na
  • p 0, a mixture of compounds of type I in which Ri represents thalloalkyl, Mi and M 2 - Na
  • cocoalkyl means a mixture of saturated and unsaturated hydrocarbon radicals, mainly C 8 -C 22 , which is part of the products obtained from the chemical processing of coconut oil.
  • titanium alkyl is meant a mixture of saturated and unsaturated hydrocarbon radicals, mainly C 8 -C 2 4, which is part of the products obtained from the chemical processing of tall oil.
  • At least one amphoteric surfactant is selected from the group consisting of betaines with the general formula
  • the substituents R t , R 2 and R 3 are selected from the group consisting of H , branched and unbranched saturated and unsaturated linear and cyclic hydrocarbon radicals, with the exception of compounds in which n is 0 and R 2 is H, with the exception of compounds in which m is 0 and
  • the antiseptic preparation comprises at least one silver salt.
  • the silver salt is selected from the group consisting of nitrate, acetate, citrate, chloride, bromide, iodide and silver sulfate.
  • the concentration of silver salt in the antiseptic preparation is from 10 "4 wt.% To 0.5 wt.%.
  • the invention also relates to a method for producing an antiseptic preparation, in which the following steps are performed: with vigorous stirring, an aqueous solution of a silver salt, a solution of at least one amphoteric surfactant and a solution of a reducing agent are mixed.
  • the claimed invention also relates to a method for using an antiseptic preparation for disinfection, in which disinfected objects are treated with a solution comprising nanosized silver particles and at least one amphoteric surfactant.
  • the claimed invention relates to a method for using an antiseptic preparation in growing plants, including spring and winter grains and legumes, vegetable open and protected soil, tree-shrub, fruit, berry, essential oil, garden, ornamental, melon, row-crop, industrial and forage crops wherein plant seeds or vegetative plants are treated with a solution comprising nanosized silver particles and at least one amphoteric surfactant.
  • the invention is illustrated by illustrative materials.
  • FIG. one An electron micrograph of nanosized silver particles stabilized by amphoteric surfactant sodium ⁇ -cocoalkyliminodipropionate is shown.
  • FIG. Figure 2 shows the diffraction pattern observed upon electron diffraction by nanoscale silver particles stabilized by amphoteric surfactant sodium ⁇ -cocoalkyliminodipropionate.
  • FIG. 3 shows the absorption spectrum in the ultraviolet region and the visible region of an antiseptic preparation based on nanoscale silver particles stabilized by amphoteric surfactant ⁇ -cocoalkyliminodipropionate sodium.
  • a solution was used containing 0.03 wt.% Amphoteric surfactant of sodium ⁇ -cocoalkyliminodipropionate and 0.0025 wt.% Nanosized silver particles.
  • the solution was obtained according to the procedure described in RF patent N ° 2 419 439 dated 05/27/2011 1 g. “Antibacterial preparation and method for its preparation”, while instead of a solution of cationic surfactant, an amphoteric surfactant solution was used. An aqueous solution of silver acetate was added dropwise with stirring to a solution of amphoteric surfactant.
  • the antiseptic preparation contained water up to 100 wt.%.
  • the resulting antiseptic drug A-1 was studied using transmission electron microscopy.
  • a typical electron micrograph of a preparation applied to a substrate is shown in FIG. one .
  • the electron microdiffraction data shown in FIG. 2 indicate that the nanosized silver particles in the preparation contain crystalline silver, since the diffraction rings are located in the same way as in the microdiffractogram of a standard sample of polycrystalline silver.
  • FIG. 3 shows the absorption spectrum of the obtained antiseptic preparation in the ultraviolet region and the visible region.
  • the spectrum is present the absorption band of nanosized silver particles in the range 310-510 nm, the occurrence of which is associated with the phenomenon of plasmon resonance, which indicates that the resulting antiseptic preparation is a colloidal solution of silver.
  • yeast cells were washed twice with distilled water, redispersed in distilled water, and precipitated at 5000 g for 15 minutes.
  • a dispersion of silver nanoparticles was added to a suspension of cells in 0.01 M citrate buffer solution with a pH of 6.0 and incubated for I hour at 30 ° C. The number of surviving cells was determined by the optical density of the suspension at a wavelength of 595 nm.
  • an antiseptic preparation based on sodium ⁇ -cocoalkyliminodipropionate and nanosized silver particles exhibits .
  • the obtained antiseptic preparation completely inhibits the growth of yeast and used bacteria and slows the growth of mycelium of used fungi by 1, 5-2 times.
  • the obtained antiseptic preparation is more resistant to aggregation under the action of electrolytes than antibacterial preparations based on silver nanoparticles and cationic surfactants.
  • a dispersion of silver nanoparticles stabilized by amphoteric surfactant ⁇ -cocoalkyliminodipropionate sodium loses aggregation stability at a concentration of potassium chloride of 0.42 mol / L
  • a dispersion of silver nanoparticles stabilized by cationic surfactant with benzyl dimethyl- [3- (myristoylamino) propyl] ammonium chloride loses resistance to aggregation at a concentration of potassium chloride of 0.24 mol / L.
  • the obtained antiseptic preparation was used to process industrial and domestic equipment and inventory.
  • the effectiveness of the antiseptic effect of the drug was evaluated by bacterial contamination of the washings from the treated objects. It was shown that the resulting antiseptic drug can be used during disinfection in industry and at home.
  • the resulting antiseptic drug can also be used to disinfect water.
  • the obtained antiseptic preparation was sprayed with seeds of spring soft wheat cultivar Altayskaya 105 in an amount of from 50 to 100 ml / t. Evaluation of the fungicidal effect of the drug was carried out by the roll method in accordance with GOST 12044-93 "Seeds of agricultural crops. Methods for determining disease contamination. "
  • the use of the obtained antiseptic preparation as a fungicide statistically significantly leads to a decrease in the affected TM of seeds by phytopathogens as compared to control experiments in which the seeds were sprayed with distilled water instead of the antiseptic preparation.
  • the resulting antiseptic preparation can be used as a fungicide in the cultivation of wheat.
  • Example 2 the antiseptic preparation A-2 was prepared analogously to Example 1, while an aqueous solution containing silver nitrate and amphoteric surfactant was added dropwise with stirring into an aqueous solution containing sodium borohydride NaBRi and amphoteric surfactant. .tica. .
  • antiseptic preparations were prepared analogously to example 1, while nitrate or silver acetate was reduced, while sodium ⁇ -cocoalkyliminodipropionate or sodium 1- (2-ethylhexyl) iminodipropionate, or sodium ⁇ -octyliminodipropionate, or ⁇ was used as an amphoteric surfactant sodium thalloalkyl iminodipropionate, or sodium ⁇ cocoalkyl aminopropionate, or a compound of type I in which Ri is cocoalkyl, M
  • the concentration of amphoteric surfactant varied in the range from 0.001 wt.% to 20 wt.%
  • the concentration of nanosized particles of silver ranged from 10 "4 wt.% To 0.5 wt.%
  • a silver salt was additionally added to the solution, while nitrate or acetate or citrate was used as the silver salt or chloride, or bromide, or iodide or silver sulfate, the concentration of silver salt ranged from 0.001 wt.% to 20 wt.%.
  • Insoluble silver salts for example, chloride, bromide, iodide and silver sulfate, were introduced into the solution in the form powder or dispersion of the powder in water with the formation, in the end, corresponding of the colloidal solution.
  • the obtained antiseptic preparations were used to process industrial and household equipment and inventory.
  • the effectiveness of the antiseptic effect of the preparations was evaluated by bacterial contamination of the washes from the treated objects. It was shown that the obtained antiseptic drugs can be used during disinfection in industry and at home.
  • the resulting antiseptic preparations can also be used to disinfect water.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Inorganic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pest Control & Pesticides (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne des préparations antiseptiques à large spectre d'action et peut être utilisée en agriculture, en médecine vétérinaire, en médecine, en cosmétologie, afin d'effectuer des désinfections sur le plan industriel ou domestique, y compris la désinfection de l'eau, de locaux, d'équipements et d'inventaires. La préparation antiseptique comprend des particules nanométriques d'argent et au moins une substance tensioactive amphotérique. Afin de produire la préparation antiseptique, une solution aqueuse de sel d'argent est mélangée par agitation intensive à une solution comprenant au moins une substance tensioactive amphotérique et à une solution de réducteur.
PCT/RU2013/000477 2013-06-10 2013-06-10 Préparation antiseptique, et procédés de production et d'utilisation Ceased WO2014200380A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/RU2013/000477 WO2014200380A1 (fr) 2013-06-10 2013-06-10 Préparation antiseptique, et procédés de production et d'utilisation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/RU2013/000477 WO2014200380A1 (fr) 2013-06-10 2013-06-10 Préparation antiseptique, et procédés de production et d'utilisation

Publications (1)

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WO2014200380A1 true WO2014200380A1 (fr) 2014-12-18

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITVE20140040A1 (it) * 2014-07-07 2014-07-07 Materiale per energizzare gli alimenti e per migliorare la prestazione fisica ed il benessere individuale
CN115138857A (zh) * 2022-07-21 2022-10-04 四川省伊洁士医疗科技有限公司 一种纳米银颗粒、制备方法及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008137632A1 (fr) * 2007-05-04 2008-11-13 The Procter & Gamble Company Compositions antimicrobiennes, produits et procédés d'utilisation
RU2419439C1 (ru) * 2009-10-29 2011-05-27 Юрий Андреевич Крутяков Антибактериальный препарат и способ его получения
RU2460797C2 (ru) * 2006-07-05 2012-09-10 Янссен Фармацевтика Н.В. Способ получения композиции, содержащей коллоидное наносеребро или нанозолото (варианты)
RU2480203C2 (ru) * 2011-04-22 2013-04-27 Общество с ограниченной ответственностью "Нанобиотех" Антибактериальная композиция и способ ее получения

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2460797C2 (ru) * 2006-07-05 2012-09-10 Янссен Фармацевтика Н.В. Способ получения композиции, содержащей коллоидное наносеребро или нанозолото (варианты)
WO2008137632A1 (fr) * 2007-05-04 2008-11-13 The Procter & Gamble Company Compositions antimicrobiennes, produits et procédés d'utilisation
RU2419439C1 (ru) * 2009-10-29 2011-05-27 Юрий Андреевич Крутяков Антибактериальный препарат и способ его получения
RU2480203C2 (ru) * 2011-04-22 2013-04-27 Общество с ограниченной ответственностью "Нанобиотех" Антибактериальная композиция и способ ее получения

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITVE20140040A1 (it) * 2014-07-07 2014-07-07 Materiale per energizzare gli alimenti e per migliorare la prestazione fisica ed il benessere individuale
CN115138857A (zh) * 2022-07-21 2022-10-04 四川省伊洁士医疗科技有限公司 一种纳米银颗粒、制备方法及其应用
CN115138857B (zh) * 2022-07-21 2023-12-22 四川省伊洁士医疗科技有限公司 一种纳米银颗粒、制备方法及其应用

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