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WO2014105246A3 - Système de tri nano-fluidique pour la synthèse de gène et produits de réaction de pcr - Google Patents

Système de tri nano-fluidique pour la synthèse de gène et produits de réaction de pcr Download PDF

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Publication number
WO2014105246A3
WO2014105246A3 PCT/US2013/063404 US2013063404W WO2014105246A3 WO 2014105246 A3 WO2014105246 A3 WO 2014105246A3 US 2013063404 W US2013063404 W US 2013063404W WO 2014105246 A3 WO2014105246 A3 WO 2014105246A3
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WIPO (PCT)
Prior art keywords
substrate
microchannel
membrane
nanopore
nanofluidic
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Ceased
Application number
PCT/US2013/063404
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English (en)
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WO2014105246A2 (fr
Inventor
Carlos Andres AGUILAR
Tarun Kumar JAIN
Rohit N. Karnik
Peter A. Carr
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Massachusetts Institute of Technology
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Massachusetts Institute of Technology
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Priority to US14/433,471 priority Critical patent/US10065154B2/en
Publication of WO2014105246A2 publication Critical patent/WO2014105246A2/fr
Publication of WO2014105246A3 publication Critical patent/WO2014105246A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/02—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/42—Electrodialysis; Electro-osmosis ; Electro-ultrafiltration; Membrane capacitive deionization
    • B01D61/44—Ion-selective electrodialysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/005—Microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
    • B01D67/0081—After-treatment of organic or inorganic membranes
    • B01D67/0088—Physical treatment with compounds, e.g. swelling, coating or impregnation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
    • B01D67/0081—After-treatment of organic or inorganic membranes
    • B01D67/009—After-treatment of organic or inorganic membranes with wave-energy, particle-radiation or plasma
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/12—Composite membranes; Ultra-thin membranes
    • B01D69/125—In situ manufacturing by polymerisation, polycondensation, cross-linking or chemical reaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/02—Inorganic material
    • B01D71/0215—Silicon carbide; Silicon nitride; Silicon oxycarbide
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B32—LAYERED PRODUCTS
    • B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
    • B32B37/00—Methods or apparatus for laminating, e.g. by curing or by ultrasonic bonding
    • B32B37/14—Methods or apparatus for laminating, e.g. by curing or by ultrasonic bonding characterised by the properties of the layers
    • B32B37/26—Methods or apparatus for laminating, e.g. by curing or by ultrasonic bonding characterised by the properties of the layers with at least one layer which influences the bonding during the lamination process, e.g. release layers or pressure equalising layers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1023—Microstructural devices for non-optical measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2323/00—Details relating to membrane preparation
    • B01D2323/30—Cross-linking
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/02—Details relating to pores or porosity of the membranes
    • B01D2325/028—Microfluidic pore structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/26—Electrical properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1028—Sorting particles

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Molecular Biology (AREA)
  • Manufacturing & Machinery (AREA)
  • Urology & Nephrology (AREA)
  • Water Supply & Treatment (AREA)
  • Nanotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Electrochemistry (AREA)
  • Plasma & Fusion (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

L'invention concerne des dispositifs et des procédés qui intègrent des technologies de nanopore et micro-fluidiques pour l'enregistrement de caractéristiques moléculaires de molécules individuelles telles que, par exemple, des biomolécules.
PCT/US2013/063404 2012-10-05 2013-10-04 Système de tri nano-fluidique pour la synthèse de gène et produits de réaction de pcr Ceased WO2014105246A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/433,471 US10065154B2 (en) 2012-10-05 2013-10-04 Nanofluidic sorting system for gene synthesis and pcr reaction products

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261710155P 2012-10-05 2012-10-05
US61/710,155 2012-10-05

Publications (2)

Publication Number Publication Date
WO2014105246A2 WO2014105246A2 (fr) 2014-07-03
WO2014105246A3 true WO2014105246A3 (fr) 2014-08-07

Family

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Family Applications (1)

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PCT/US2013/063404 Ceased WO2014105246A2 (fr) 2012-10-05 2013-10-04 Système de tri nano-fluidique pour la synthèse de gène et produits de réaction de pcr

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Country Link
US (1) US10065154B2 (fr)
WO (1) WO2014105246A2 (fr)

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EP3971300B1 (fr) * 2014-10-16 2024-06-05 Oxford Nanopore Technologies plc Tri de polymères
SG10201906952QA (en) 2014-12-19 2019-09-27 Univ Ottawa Integrating nanopore sensors within microfluidic channel arrays using controlled breakdown
GB201508669D0 (en) * 2015-05-20 2015-07-01 Oxford Nanopore Tech Ltd Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown
CN105158313B (zh) * 2015-07-09 2016-06-22 济南大学 一种基于二硫化钼/镍钯合金纳米复合材料构建的无标记电化学免疫传感器的制备方法
CN105158453B (zh) * 2015-07-09 2017-01-25 济南大学 一种用于壬基酚检测的无标记电化学免疫传感器的制备方法
US9958362B1 (en) * 2015-10-02 2018-05-01 The Florida State University Research Foundation, Inc. Microscope sample preparation device
AU2017237981B2 (en) 2016-03-21 2022-02-10 Nooma Bio, Inc. Wafer-scale assembly of insulator-membrane-insulator devices for nanopore sensing
US10160987B2 (en) 2016-04-07 2018-12-25 Rebecca F. McClure Composition and method for processing DNA
CN106410132B (zh) * 2016-11-09 2019-08-06 上海大学 二维片状MoS2@石墨烯复合纳米材料及其制备方法
IL308120B2 (en) 2017-06-14 2024-12-01 Vertex Pharma Devices and methods for delivering therapeutics
CN107441948A (zh) * 2017-09-21 2017-12-08 西安工程大学 一种镍负载型疏水性SiO2复合膜及其制备方法
WO2019068059A1 (fr) * 2017-09-29 2019-04-04 Semma Therapeutics, Inc. Dispositif de logement de cellules
JP6959121B2 (ja) * 2017-12-05 2021-11-02 株式会社日立ハイテク 孔形成方法及び孔形成装置
CN108181366B (zh) * 2017-12-13 2019-09-27 河南大学 以二氧化钛-二硫化钼-金三元复合材料为支架的光电化学适配体传感器的构建方法
CN108014653A (zh) * 2018-01-22 2018-05-11 福州大学 一种光照增强油水分离性能的氧化石墨烯-半导体复合膜
US12383902B2 (en) * 2018-02-07 2025-08-12 Jaydeep BHATTACHARYA Low cost microfluidic device for DNA/RNA isolation, purification and amplification using chip based PCR/RT-PCR for biosensing applications
CN108607582B (zh) * 2018-05-29 2020-11-24 潍坊学院 二硫化钼/还原性氧化石墨烯/镍纳米粒子复合材料、其制备方法及应用
CN108872341B (zh) * 2018-06-30 2019-05-28 福建师范大学 一种基于双敏化结构的甲胎蛋白光电化学竞争免疫型传感器的制备及应用
CN109277006A (zh) * 2018-09-30 2019-01-29 天津市金鳞水处理科技有限公司 一种聚偏氟乙烯改性复合膜的制备方法
US11249067B2 (en) * 2018-10-29 2022-02-15 Applied Materials, Inc. Nanopore flow cells and methods of fabrication
CN110458786B (zh) * 2019-07-31 2022-05-17 重庆邮电大学 一种先验gan模型医学影像生成方法
WO2021026382A1 (fr) * 2019-08-06 2021-02-11 Nooma Bio, Inc. Balayage de polynucléotide commandé par logique pour cartographier des caractéristiques dans un dispositif à nanopores
WO2021113750A1 (fr) * 2019-12-05 2021-06-10 Nooma Bio, Inc. Dispositif à pores multiples avec applications de tri de matériaux
CN111393034A (zh) * 2020-05-26 2020-07-10 北京石墨烯研究院 一种石墨烯玻璃的连续生产系统及方法
US20230249174A1 (en) * 2020-07-08 2023-08-10 Arizona Board Of Regents On Behalf Of Arizona State University Methods to construct sharp and stable tip contacts with nanometer precision in a confined nanoscale space between two microfluidic chambers
WO2022010935A2 (fr) 2020-07-08 2022-01-13 Quan Qing Procédé de construction de dispositifs de jonction à effet tunnel magnétique et utilisation d'un dispositif pour une caractérisation de transport dépendant du spin
US12241857B2 (en) 2021-03-16 2025-03-04 Arizona Board Of Regents On Behalf Of Arizona State University High density and multiplexed nanopore devices with transverse tunneling junction for biomolecule detection and sequencing
CN114875378B (zh) * 2022-05-08 2024-07-02 中国科学院重庆绿色智能技术研究院 一种竖直结构二硒化钨阵列薄膜结构的细胞筛分装置的制备方法
CN114887497B (zh) * 2022-05-20 2023-07-25 镇江市高等专科学校 一种基于废弃塑料的静电纺丝油水分离膜材料的制备方法
KR20240077064A (ko) * 2022-11-24 2024-05-31 성균관대학교산학협력단 Pcr 시스템 및 이의 제조 방법
CN116425190B (zh) * 2023-04-04 2024-04-12 华东师范大学 一种基于微流控芯片原位生长氧化锌纳米棒的方法
GB2641042A (en) * 2024-05-13 2025-11-19 Izon Science Ltd Solid state nanopores for nanoparticle sensing
CN119113132B (zh) * 2024-11-15 2025-03-18 上海交通大学医学院附属仁济医院 一种光热肿瘤免疫激活剂及其制备方法和使用方法

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US20150283514A1 (en) 2015-10-08
WO2014105246A2 (fr) 2014-07-03

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