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WO2014192037A1 - Elément filtrant anti-aérosol ayant des activités antimicrobiennes pour masques nbc - Google Patents

Elément filtrant anti-aérosol ayant des activités antimicrobiennes pour masques nbc Download PDF

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Publication number
WO2014192037A1
WO2014192037A1 PCT/IT2014/000150 IT2014000150W WO2014192037A1 WO 2014192037 A1 WO2014192037 A1 WO 2014192037A1 IT 2014000150 W IT2014000150 W IT 2014000150W WO 2014192037 A1 WO2014192037 A1 WO 2014192037A1
Authority
WO
WIPO (PCT)
Prior art keywords
filter
chloride
chloroallyl
antimicrobial
azoniaadamantane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IT2014/000150
Other languages
English (en)
Inventor
Giuseppe Galli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
OFFICINE MECCANICHE GALLI Srl
Original Assignee
OFFICINE MECCANICHE GALLI Srl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by OFFICINE MECCANICHE GALLI Srl filed Critical OFFICINE MECCANICHE GALLI Srl
Publication of WO2014192037A1 publication Critical patent/WO2014192037A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62B—DEVICES, APPARATUS OR METHODS FOR LIFE-SAVING
    • A62B23/00—Filters for breathing-protection purposes
    • A62B23/02—Filters for breathing-protection purposes for respirators
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62B—DEVICES, APPARATUS OR METHODS FOR LIFE-SAVING
    • A62B19/00—Cartridges with absorbing substances for respiratory apparatus

Definitions

  • the present invention relates to a filter element for NBC mask seal cover and more in particular an anti- aerosol filter to be used inside the filtering elements for NBC mask seal cover, able to inhibit bacterial contamination and to avoid the risk of bioporation.
  • bioporation indicates the deterioration of a filtering _ medium due to microbial growth of microorganisms, retained by absolute or high efficiency filters, survive and proliferate in situ.
  • the atmospheric air acts as a vehicle responsible for the non-uniform distribution of each suspended particle and therefore also for the distribution of airborne microbial cells and/or spore forms whose concentration is subject to strong variations correlated to the habitat, and especially to the presence of humans.
  • Aim of the present invention is to solve such problem by providing a filter having antimicrobial activity and preventing microorganisms colonization, thus eliminating the risk of bioporation.
  • Antimicrobial treatments for air filtration products have been successful by time: antimicrobial agents tasking to destroy or inhibit the microorganisms growth have been widely used in applications on commercial and industrial surfaces, such as walls, floors, etc..
  • antimicrobial agents tasking to destroy or inhibit the microorganisms growth have been widely used in applications on commercial and industrial surfaces, such as walls, floors, etc..
  • An anti NBC filtering element normally contains a core of activated carbon to trap dust and an anti- aerosol package preferably consisting of a glass microfiber paper cartridge.
  • the aerosol package is added with an antimicrobial agent selected from those most active, compatible with its machining process.
  • antimicrobial agents are available on the market, and among these, for example, various plant extracts, quaternary ammonium compounds, polyhexamethylene guanidine hydrochloride, methylparaben were recognized as compatible.
  • a phosphated quaternary amine complex highly soluble in water and slightly soluble in non-aqueous solvents, being the pH of the aqueous solution between 6.5 and 7.7, and cis-1- (3-chloroallyl) -3, 5, 7-triaza-l azonia-adamantane chloride or a related product selected from: crude 1- ( 3-chloroallyl) -3, 5, 7-triaza-l-azonia-adamantane, N- (3- chloroallyl) hexaminium chloride, chloroallyl methanamine chloride, chloride azonia-adamantane chloride, N- (3-chloroallyl) -hexaminium chloride, azonia-adamantane cloroallyl methanamine chloride, Dowicil ® 75, 150, 200, Preventol ® Dl dia
  • figure 1 is a perspective view in diametrical section through the center of the attack of a anti NBC filter wherein the attack is central.
  • a filtering element produced by the Applicant is illustrated, subject matter of the Italian patent 1359749, which is equipped with a central attack 2 whose symmetry axis coincides with that of the main filter itself, where the aerosol package 4 made of a paper cartridge with glass microfiber, between a first and a second perforated disc, respectively 8 and 10, is attached and sealed to the rear base Bl by means of non-toxic glue.
  • the active carbon filter 12 is positioned in the front part of the element.
  • the glass microfiber filter 4 has been impregnated with an aqueous solution of acrylic resin, anti-foam, and with an antimicrobial agent at concentration between 18,000 and 45,000 ppm.
  • This procedure is performed in order to verify the effectiveness of the antimicrobial treatment of the filter of the invention, referred as ULPA U15, included into the filtering element produced and patented (Italian Patent No. 1359749) by the Applicant, to inhibit contamination with no risk of bacterial bioporation .
  • This procedure subjects the filtering media to a more severe treatment with respect to the normal use conditions of the filters.
  • test was performed according to the method reported in Verdenelli et al. 2003 "Efficacy of antimicrobial filter treatments on microbial colonization of air panel filters”. Journal of Applied Microbiology 94: 9-15.
  • the filtering element was connected to a vacuum pump and subjected to 10 hours of operation with an air flow set at 20 liters per minute in a room of 36 m 3 wherein a standard powder consisting of 5 g of Sabouraud Dextrose Agar (Oxoid) and 5 g of Tryptone Soya Agar (Oxoid) was previously dispersed.
  • a standard powder consisting of 5 g of Sabouraud Dextrose Agar (Oxoid) and 5 g of Tryptone Soya Agar (Oxoid) was previously dispersed.
  • Such simulation was aimed to simulate a condition of used filter due to the deposition of a layer of organic media onto the ULPA filter.
  • a microbial suspension of the strains reported in Table 1 at concentration of 6 x 10 8 cells/ml was prepared.
  • the surface of the ULPA filter of the filtering element was inoculated with 1 ml of the microbial suspension and the ULPA filter has been incubated in an incubator at about 30 ⁇ 2 °C and relative humidity above 60%.
  • Microbial proliferation of the ULPA filters was verified through the use of a stereomicroscope (GSZ2, Ascania, Germany) .
  • the bioporation was verified by connecting the filtering element to an olive-shaped filter holder containing a sterile filtering membrane. A vacuum pump was applied and the air was passed through the filter for 5 minutes at rate of 2 liters per minute. After filtration the sterile filtering membrane was placed to incubate on a Petri dish plate containing Tryptone Soya Agar (OXOID) for the bacteria counting and Potato Dextrose Agar (OXOID) for the mold counting, for 7 days at 25 ⁇ 2 °C.
  • OXOID Tryptone Soya Agar
  • OXOID Potato Dextrose Agar
  • Candida albicans ATCC 10291 Candida albicans ATCC 10291
  • the antimicrobial ULPA U15 filters contained in the filtering elements have shown a good antimicrobial activity towards the two test bacterial strains reporting values of diameter of inhibition greater than 9 mm (value considered as positive for the antimicrobial activity since it corresponds to the diameter of the tested filter punches) .
  • the tested antimicrobial ULPA U15 filters contained in the filtering elements have demonstrated both efficacy of the antimicrobial treatment of the U15 ULPA filter in preventing microbial colonization and efficiency in preventing the downstream release of the microbial agents that eventually deposit on the filter during use.
  • This procedure is performed to determine the percentage of bacterial filtration efficiency (% BFE) of filtering elements, through the calculation of the ratio between the tested bacterial cells compared to those passing through the filter. This procedure subjects the filtering media to a more severe treatment than the normal use condition of the filters.
  • the bacterial strain Staphylococcus aureus ATCC 25923 was used for the efficiency test of bacteria filtration by the anti-aerosol filtering - element .
  • a bacterial suspension of Staphylococcus aureus at a concentration of about 5 x 10 5 Colony Forming Units (CFU) /ml was prepared from a 5 McFarland concentration, equal to 15xl0 8 cells/ml by diluting in Peptone Water.
  • the bacterial suspension (5 ml) was placed in the nebulizer connected to a pump for the production of an aerosol.
  • the aerosol is conveyed with a flow rate of 28.3 1/min to the filtering element containing the antibacterial ULPA filter to be tested.
  • the filtering element was placed with the outer surface facing the aerosol. Before to be tested the filtering elements were conditioned at 20 ⁇ 2 °C at a relative humidity of 65 ⁇ 2% for 4 hours) .
  • Bacterial filtration efficiency is calculated as a percentage using the following equation:
  • T average of total counts of the plates for five filtering elements.
  • This procedure is performed in order to determine the percentage of spores filtration efficiency (% SFE) of filtering elements, through the calculation of the ratio between tested bacterial spores compared to those that pass through the filter. This procedure subjects the filtering media to a more severe treatment than the normal use condition of the filters.
  • a spore suspension of Bacillus globigii at concentration of about 10 spores/liter was prepared and placed in a nebulizer connected to a pump for the production of an aerosol.
  • the aerosol was conveyed with a flow rate of 30 1/min to the antibacterial filtering element containing the ULPA filter to be tested.
  • the filtering element was placed with the outer surface facing the aerosol.
  • the filtering elements were conditioned at 20 ⁇ 2 °C and relative humidity of 65+2% for 4 hours. After 1 hour of sampling the spore load was assessed onto Tryptone Soya Agar plates (OXOID) and kept in an incubator for at least 48 ⁇ 4 hrs, at a temperature of 37 ⁇ 2 °C.
  • a positive control was performed in duplicate, at least 5 filtering elements were tested and a negative control at the end of the replicates.
  • the spore filtration efficiency is calculated as a percentage using the following equation:
  • T average total counts of the plates for the five filtering elements.
  • This procedure is performed in order to determine the percentage of virus filtration efficiency (% VFE) of filtering elements, through the calculation of the ratio between tested viral particles compared to those passing through the filter.
  • This procedure subjects the filtering media to a more severe treatment than the normal use conditions of the filters.
  • the test is performed according to the method of the "American Society for Testing and Medias Standards" (ASTM) F2101.
  • the bacteriophage phi-X 174 was used for the of viruses filtration efficiency test by the filtering element.
  • a viral suspension with concentration of 15xl0 8 virus/ml was prepared.
  • the used instrumentation is consisting of:
  • the virus suspension (5 ml) was placed in the nebulizer connected to a pump for the production of an aerosol.
  • the aerosol is conveyed to the filtering element to be tested with a flow rate of 30 l/min.
  • the aerosol passes through the filter and the viral particles, eventually released, are collected and captured by a bubbler containing 5 ml of sterile peptone water (Figure 1) .
  • the filters were conditioned at 21 ⁇ 5 °C at a relative humidity of 85 ⁇ 5% for 4 hours. After a certain sampling time (2 minutes) the filtration efficiency of the assayed filter toward the test virus was evaluated by assessing the viral load both before and after the filtration process by the method of plate count of lysis plaques (PFU) .
  • PFU plate count of lysis plaques
  • the results obtained are reported in Table 5.
  • the filtration efficiency was calculated as percentage value of the difference between the number of viral particles impacting the filter compared to the number of viral particles passing downstream of the filtering media according to the following formula:
  • VFE% C-TIC x 100
  • T number of viral particles passing downstream the filter.

Landscapes

  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Business, Economics & Management (AREA)
  • Emergency Management (AREA)
  • Pulmonology (AREA)
  • Filtering Materials (AREA)
  • Respiratory Apparatuses And Protective Means (AREA)

Abstract

L'invention concerne un ensemble filtre aérosol (4) constitué de microfibres de verre, dans lequel un agent antimicrobien a été ajouté à la résine acrylique pendant le processus de fabrication pour empêcher la formation d'un film biologique et réduire la possibilité de bioporation du filtre lui-même.
PCT/IT2014/000150 2013-05-29 2014-05-29 Elément filtrant anti-aérosol ayant des activités antimicrobiennes pour masques nbc Ceased WO2014192037A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITRM2013A000309 2013-05-29
IT000309A ITRM20130309A1 (it) 2013-05-29 2013-05-29 "elemento filtrante antiaerosol per maschere nbc dotato di attivita' antimicrobica"

Publications (1)

Publication Number Publication Date
WO2014192037A1 true WO2014192037A1 (fr) 2014-12-04

Family

ID=49085148

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT2014/000150 Ceased WO2014192037A1 (fr) 2013-05-29 2014-05-29 Elément filtrant anti-aérosol ayant des activités antimicrobiennes pour masques nbc

Country Status (2)

Country Link
IT (1) ITRM20130309A1 (fr)
WO (1) WO2014192037A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220032092A1 (en) * 2018-12-23 2022-02-03 Beth-El Zikhron Yaaqov Industries Ltd. Nbc filtration systems
CN114459981A (zh) * 2021-12-22 2022-05-10 河北省药品医疗器械检验研究院(河北省化妆品检验研究中心) 一种用于检测口罩细菌过滤效率的方法
US20220186280A1 (en) * 2020-12-15 2022-06-16 Sanko Tekstil Isletmeleri San. Ve Tic. A.S. Method for testing bacterial filtration efficacy of fabrics

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5240478A (en) * 1992-06-26 1993-08-31 Messina Gary D Self-contained, portable room air treatment apparatus and method therefore
US20080066739A1 (en) * 2006-09-20 2008-03-20 Lemahieu Edward Methods and systems of delivering medication via inhalation
WO2008081489A1 (fr) * 2007-01-05 2008-07-10 Officine Meccaniche Galli S.R.L. Filtre nbc
WO2010096285A2 (fr) * 2009-02-20 2010-08-26 3M Innovative Properties Company Voile électret antimicrobien
WO2013010535A2 (fr) * 2011-07-18 2013-01-24 Ding Haomin Filtre, notamment filtre pour air respiré

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5240478A (en) * 1992-06-26 1993-08-31 Messina Gary D Self-contained, portable room air treatment apparatus and method therefore
US20080066739A1 (en) * 2006-09-20 2008-03-20 Lemahieu Edward Methods and systems of delivering medication via inhalation
WO2008081489A1 (fr) * 2007-01-05 2008-07-10 Officine Meccaniche Galli S.R.L. Filtre nbc
WO2010096285A2 (fr) * 2009-02-20 2010-08-26 3M Innovative Properties Company Voile électret antimicrobien
WO2013010535A2 (fr) * 2011-07-18 2013-01-24 Ding Haomin Filtre, notamment filtre pour air respiré

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220032092A1 (en) * 2018-12-23 2022-02-03 Beth-El Zikhron Yaaqov Industries Ltd. Nbc filtration systems
US11938351B2 (en) * 2018-12-23 2024-03-26 Beth-El Zikhron Yaaqov Industries Ltd. NBC filtration systems
US20220186280A1 (en) * 2020-12-15 2022-06-16 Sanko Tekstil Isletmeleri San. Ve Tic. A.S. Method for testing bacterial filtration efficacy of fabrics
CN114459981A (zh) * 2021-12-22 2022-05-10 河北省药品医疗器械检验研究院(河北省化妆品检验研究中心) 一种用于检测口罩细菌过滤效率的方法

Also Published As

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ITRM20130309A1 (it) 2014-11-30

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