[go: up one dir, main page]

WO2014063051A3 - Témoins d'amplification d'acides nucléiques, kits et leurs procédés d'utilisation - Google Patents

Témoins d'amplification d'acides nucléiques, kits et leurs procédés d'utilisation Download PDF

Info

Publication number
WO2014063051A3
WO2014063051A3 PCT/US2013/065681 US2013065681W WO2014063051A3 WO 2014063051 A3 WO2014063051 A3 WO 2014063051A3 US 2013065681 W US2013065681 W US 2013065681W WO 2014063051 A3 WO2014063051 A3 WO 2014063051A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
methods
kits
rna
acid amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2013/065681
Other languages
English (en)
Other versions
WO2014063051A2 (fr
Inventor
Christian Leutenegger
Michael John ANGELICHIO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Idexx Laboratories Inc
Original Assignee
Idexx Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Idexx Laboratories Inc filed Critical Idexx Laboratories Inc
Priority to ES13786570.5T priority Critical patent/ES2685710T3/es
Priority to EP13786570.5A priority patent/EP2909339B1/fr
Priority to US14/436,739 priority patent/US10174373B2/en
Priority to EP18174494.7A priority patent/EP3415638B1/fr
Priority to DK13786570.5T priority patent/DK2909339T3/en
Publication of WO2014063051A2 publication Critical patent/WO2014063051A2/fr
Publication of WO2014063051A3 publication Critical patent/WO2014063051A3/fr
Anticipated expiration legal-status Critical
Priority to US16/227,732 priority patent/US11725242B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention concerne des composants et des procédés de dosage par réaction en chaîne de la polymérase. Les dosages réduisent au minimum à la fois la manipulation du matériel et le temps passé à soumettre les échantillons. Par exemple, une seule paire de polynucléotides à témoin positif interne (SPC) peut offrir un moyen de garantir une purification adéquate des acides nucléiques pour des cibles d'essai à la fois de type ARN et ADN. De plus, les conditions de thermocyclage standards pour tous les tests diagnostiques permettent à l'utilisateur de tester à la fois des cibles ARN et ADN côte-à-côte.
PCT/US2013/065681 2012-10-18 2013-10-18 Témoins d'amplification d'acides nucléiques, kits et leurs procédés d'utilisation Ceased WO2014063051A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
ES13786570.5T ES2685710T3 (es) 2012-10-18 2013-10-18 Kits que comprenden controles para amplificación de ácido nucleico
EP13786570.5A EP2909339B1 (fr) 2012-10-18 2013-10-18 Kits avec témoins d'amplification d'acides nucléiques
US14/436,739 US10174373B2 (en) 2012-10-18 2013-10-18 Nucleic acid amplification controls and kits and methods of use thereof
EP18174494.7A EP3415638B1 (fr) 2012-10-18 2013-10-18 Commandes de l'amplification d'acides nucléiques et kits et leurs procédés d'utilisation
DK13786570.5T DK2909339T3 (en) 2012-10-18 2013-10-18 Kits comprehensive controls for nucleic acid amplification
US16/227,732 US11725242B2 (en) 2012-10-18 2018-12-20 Nucleic acid amplification controls and kits and methods of use thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201261715596P 2012-10-18 2012-10-18
US61/715,596 2012-10-18
US201361790854P 2013-03-15 2013-03-15
US61/790,854 2013-03-15

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US14/436,739 A-371-Of-International US10174373B2 (en) 2012-10-18 2013-10-18 Nucleic acid amplification controls and kits and methods of use thereof
US16/227,732 Division US11725242B2 (en) 2012-10-18 2018-12-20 Nucleic acid amplification controls and kits and methods of use thereof

Publications (2)

Publication Number Publication Date
WO2014063051A2 WO2014063051A2 (fr) 2014-04-24
WO2014063051A3 true WO2014063051A3 (fr) 2014-06-26

Family

ID=49546617

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/065681 Ceased WO2014063051A2 (fr) 2012-10-18 2013-10-18 Témoins d'amplification d'acides nucléiques, kits et leurs procédés d'utilisation

Country Status (5)

Country Link
US (2) US10174373B2 (fr)
EP (2) EP2909339B1 (fr)
DK (1) DK2909339T3 (fr)
ES (1) ES2685710T3 (fr)
WO (1) WO2014063051A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3208333B1 (fr) * 2014-10-17 2020-12-16 National University Corporation Tokyo Medical and Dental University Procédé de détection et de quantification d'acide nucléique cible dans un échantillon pour essai utilisant le nouvel acide nucléique de contrôle positif
EP3790988A4 (fr) * 2018-05-10 2021-12-01 Seegene, Inc. Procédé de détection de micro-organisme intestinal dans un échantillon en utilisant la flore intestinale normale comme témoin interne
JP7272202B2 (ja) * 2019-09-20 2023-05-12 株式会社島津製作所 標的核酸の検査方法および検査装置
CN111534634A (zh) * 2020-07-07 2020-08-14 南阳师范学院 一种ⅱ型犬腺病毒的可视化等温扩增检测试剂及其应用
CN114891868A (zh) * 2022-05-31 2022-08-12 广州市金圻睿生物科技有限责任公司 一种基于ngs平台的微生物定量方法及试剂盒
CN117385100B (zh) * 2023-10-09 2024-07-09 华中农业大学 一种犬呼吸道冠状病毒的荧光定量pcr检测试剂盒
TWI891218B (zh) * 2024-01-24 2025-07-21 台達電子工業股份有限公司 核酸檢測系統之質檢套組及其質檢方法與用途

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998058083A2 (fr) * 1997-06-16 1998-12-23 Medical College Of Ohio Procede de mesure quantitative de l'expression genique au moyen d'une reaction en chaine de la polymerase-transcriptase inverse multiplexe concurrente
EP1319716A1 (fr) * 2000-09-22 2003-06-18 Hitachi, Ltd. Procede d'analyse d'acide nucleique
WO2003083051A2 (fr) * 2002-03-28 2003-10-09 Medical College Of Ohio Amplification en chaine par transcriptase-polymerase inverse normalise multiplex pour l'evaluation de l'expression genique dans de petits echantillons biologiques
WO2004104229A2 (fr) * 2003-05-16 2004-12-02 Vickery Michael C L Molecule d'acide nucleique de controle interne pour systemes d'amplification d'acide nucleique
WO2005040396A2 (fr) * 2003-10-16 2005-05-06 Genomic Health, Inc. Systeme d'essai qrt-pcr pour le profilage d'expression genetique
US20120115154A1 (en) * 2004-12-23 2012-05-10 Greg Hampikian Reference markers for biological samples

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4973679A (en) 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
FR2596761B1 (fr) 1986-04-08 1988-05-20 Commissariat Energie Atomique Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
ES2128323T3 (es) 1990-07-24 1999-05-16 Hoffmann La Roche Reduccion de la amplificacion no-especifica durante la amplificacion in vitro del acido nucleico empleando bases de acido nucleico modificadas.
FR2690691B1 (fr) 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
JP2909216B2 (ja) 1994-04-29 1999-06-23 パーキン‐エルマー コーポレイション 核酸増幅生成物のリアルタイム検出装置
US5736333A (en) 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
EP2354251B1 (fr) 1997-04-03 2014-10-15 Life Technologies Corporation Compositions et procédés d'amplification en chaine par polymerase-transcriptase inverse (RT-PCR)
US5952202A (en) 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification
AUPQ909000A0 (en) * 2000-07-28 2000-08-24 University Of Sydney, The A method of detecting microorganisms
US20030124512A1 (en) * 2000-10-18 2003-07-03 Lieven Stuyver Simultaneous quantification of nucleic acids in diseased cells
JP4808365B2 (ja) * 2001-03-02 2011-11-02 ユニバーシティ オブ ピッツバーグ オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション Ｐｃｒ法
US20030211503A1 (en) * 2001-10-01 2003-11-13 Carpentieri David F. Materials and methods for the diagnosis of pediatric tumors
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
WO2006096224A2 (fr) 2004-12-20 2006-09-14 Cytyc Corporation Procede de detection simultanee d'arn et d'adn de papillomavirus humain dans un echantillon
US8785130B2 (en) * 2005-07-07 2014-07-22 Bio-Id Diagnostic Inc. Use of markers including nucleotide sequence based codes to monitor methods of detection and identification of genetic material
US7981606B2 (en) 2005-12-21 2011-07-19 Roche Molecular Systems, Inc. Control for nucleic acid testing
WO2008001156A1 (fr) * 2006-06-26 2008-01-03 Centre Regional De Lutte Contre Le Cancer - Centre Francois Baclesse TRAITEMENT D'UN CANCER UTILISANT UN siNA SPÉCIFIQUE À BcI-XL
US8980562B1 (en) * 2007-06-12 2015-03-17 Physicians Reference Laboratory Method of simultaneous detection and typing of human papilloma viruses
US9834815B2 (en) 2009-03-25 2017-12-05 Life Technologies Corporation Discriminatory positive/extraction control DNA

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998058083A2 (fr) * 1997-06-16 1998-12-23 Medical College Of Ohio Procede de mesure quantitative de l'expression genique au moyen d'une reaction en chaine de la polymerase-transcriptase inverse multiplexe concurrente
EP1319716A1 (fr) * 2000-09-22 2003-06-18 Hitachi, Ltd. Procede d'analyse d'acide nucleique
WO2003083051A2 (fr) * 2002-03-28 2003-10-09 Medical College Of Ohio Amplification en chaine par transcriptase-polymerase inverse normalise multiplex pour l'evaluation de l'expression genique dans de petits echantillons biologiques
WO2004104229A2 (fr) * 2003-05-16 2004-12-02 Vickery Michael C L Molecule d'acide nucleique de controle interne pour systemes d'amplification d'acide nucleique
WO2005040396A2 (fr) * 2003-10-16 2005-05-06 Genomic Health, Inc. Systeme d'essai qrt-pcr pour le profilage d'expression genetique
US20120115154A1 (en) * 2004-12-23 2012-05-10 Greg Hampikian Reference markers for biological samples

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
WILLEY J C ET AL: "Expression measurement of many genes simultaneously by quantitative RT-PCR using standardized mixtures of competitive templates", AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, AMERICAN LUNG ASSOCIATION, NEW YORK, NY, US, vol. 19, 1 January 1998 (1998-01-01), pages 6 - 17, XP002972416, ISSN: 1044-1549 *
ZIMMERMANN K ET AL: "TECHNICAL ASPECTS OF QUANTITATIVE COMPETITIVE PCR", BIOTECHNIQUES, INFORMA HEALTHCARE, US, vol. 21, no. 2, 1 August 1996 (1996-08-01), pages 268, XP000597689, ISSN: 0736-6205 *

Also Published As

Publication number Publication date
DK2909339T3 (en) 2018-08-13
US10174373B2 (en) 2019-01-08
EP3415638A1 (fr) 2018-12-19
EP2909339B1 (fr) 2018-05-30
US20150275294A1 (en) 2015-10-01
WO2014063051A2 (fr) 2014-04-24
EP3415638B1 (fr) 2025-10-08
ES2685710T3 (es) 2018-10-10
EP2909339A2 (fr) 2015-08-26
US20190112658A1 (en) 2019-04-18
US11725242B2 (en) 2023-08-15

Similar Documents

Publication Publication Date Title
WO2014063051A3 (fr) Témoins d'amplification d'acides nucléiques, kits et leurs procédés d'utilisation
WO2012094459A3 (fr) Cartouches d'essai et leurs procédés d'utilisation
EP4049757A3 (fr) Systèmes et procédés de diagnostic
WO2012129157A3 (fr) Système pour mettre en œuvre une amplification d'acide nucléique par réaction en chaîne de polymérase
HK1211260A1 (en) Method and kit for nucleic acid sequencing
GB201014805D0 (en) Microfluidics based assay device
LU91864B1 (en) Method and kit for the isolation of genomic DNA, RNA, proteins and metabolites from a single biological sample
WO2014097286A8 (fr) Systèmes et méthodes de détermination d'un état chimique
GB201317477D0 (en) Methods, compositions and kits for determing the presence/absnece of a variant nucleic acid sequence
EP4372084A3 (fr) Compositions et procédés pour l'enrichissement ciblé de séquences d'acides nucléiques et la génération de bibliothèques à haute efficacité
WO2012134602A3 (fr) Procédés et systèmes de séquençage de longs acides nucléiques
WO2012018741A3 (fr) Cartouche pouvant être mise sous pression pour réactions de polymérisation en chaîne
SG10201903822XA (en) Method for the enrichment of circulating tumor dna
EP2615457A4 (fr) Composition de réactif pour la chromatographie ou l'immunochromatographie d'acide nucléique, procédé de mesure par la chromatographie ou l'immunochromatographie d'acide nucléique, et kit de mesure par chromatographie ou immunochromatographie d'acide nucléique
WO2010107816A3 (fr) Colorants de liaison d'acides nucléiques et leurs utilisations
WO2014005125A3 (fr) Composés fluorescents et utilisation de ceux-ci
BR112014005505A2 (pt) substrato de polinucleotídeo isolado para uma enzima de ácido nucleico catalítica, método para detectar a presença de pelo menos um alvo, uso do substrato de polinucleotídeo isolado e kit
WO2012067465A3 (fr) Appareil automatique de purification d'acides nucléiques et procédé de protection d'un aérosol
EA201200907A1 (ru) Способ обнаружения генетического материала chlamydia trachomatis, зонд нуклеиновой кислоты (варианты), прямой (варианты) и обратный (варианты) праймеры пцр, их содержащий компонент пцр, содержащий вышеуказанный компонент набор и способ обнаружения генетического материала pectobacterium atrosepticium
WO2012135658A3 (fr) Conversion d'adn à séquence préservée pour séquençage optique par nanopore
MX2017000391A (es) Tecnologia de amplificacion de adn.
WO2012011660A3 (fr) Procédé de fabrication d'une plaque à micro-alvéoles intégrant un échantillon et d'une plaque d'analyse à micro-alvéoles, plaque d'analyse à micro-alvéoles et ensemble appareil pour fabriquer une plaque d'analyse à micro-alvéoles intégrant un échantillon
WO2014210199A3 (fr) Procédés de réalisation d'une amplification en chaîne par polymérase et utilisations associées
WO2013090386A3 (fr) Procédés et ensembles pour détection in situ à température ambiante d'un acide nucléique cible dans un échantillon biologique
WO2011148269A3 (fr) Méthode diagnostique

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13786570

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2013786570

Country of ref document: EP