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WO2013192530A3 - Methods and reagents for glycoproteomics - Google Patents

Methods and reagents for glycoproteomics Download PDF

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Publication number
WO2013192530A3
WO2013192530A3 PCT/US2013/047073 US2013047073W WO2013192530A3 WO 2013192530 A3 WO2013192530 A3 WO 2013192530A3 US 2013047073 W US2013047073 W US 2013047073W WO 2013192530 A3 WO2013192530 A3 WO 2013192530A3
Authority
WO
WIPO (PCT)
Prior art keywords
glycan
separation
polypeptide
glycans
reagents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2013/047073
Other languages
French (fr)
Other versions
WO2013192530A2 (en
Inventor
Richard S. LEE
Hui Zhou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boston Childrens Hospital
Original Assignee
Boston Childrens Hospital
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boston Childrens Hospital filed Critical Boston Childrens Hospital
Publication of WO2013192530A2 publication Critical patent/WO2013192530A2/en
Anticipated expiration legal-status Critical
Publication of WO2013192530A3 publication Critical patent/WO2013192530A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

Some aspects of this disclosure provide a method for separating a glycan and/or a polypeptide fraction from a mixture comprising a glycan and a polypeptide. In some embodiments, the method comprises acidifying the mixture in order to decrease non-covalent glycan/polypeptide interactions prior to separation. Reagents and devices useful for glycan and/or polypeptide separation are also provided. The technology disclosed herein allows for the rapid, cost-efficient separation and isolation of both glycans and polypeptides obtained from glycosylated proteins after release of the glycans, for example, by enzymatic digest. Uses of the disclosed technology include, for example, the separation and isolation of glycans and/or polypeptides from body fluid samples for diagnostics, and quality control of glycosylation in engineered proteins, e.g., in therapeutic antibodies.
PCT/US2013/047073 2012-06-21 2013-06-21 Methods and reagents for glycoproteomics Ceased WO2013192530A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261662893P 2012-06-21 2012-06-21
US61/662893 2012-06-21

Publications (2)

Publication Number Publication Date
WO2013192530A2 WO2013192530A2 (en) 2013-12-27
WO2013192530A3 true WO2013192530A3 (en) 2015-06-18

Family

ID=49769720

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/047073 Ceased WO2013192530A2 (en) 2012-06-21 2013-06-21 Methods and reagents for glycoproteomics

Country Status (1)

Country Link
WO (1) WO2013192530A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10436790B2 (en) 2011-09-28 2019-10-08 Waters Technologies Corporation Rapid fluorescence tagging of glycans and other biomolecules with enhanced MS signals
WO2014074756A1 (en) * 2012-11-09 2014-05-15 Children's Medical Center Corporation Methods and reagents for glycoproteomics
JP7055634B2 (en) 2014-10-30 2022-04-18 ウオーターズ・テクノロジーズ・コーポレイシヨン Rapid Preparation of Labeled Glycosylamines and Analytical Methods for Glycosylated Biomolecules Producing It
US10502720B2 (en) 2014-11-13 2019-12-10 Waters Technologies Corporation Methods for liquid chromatography calibration for rapid labeled N-glycans
WO2017222955A1 (en) 2016-06-21 2017-12-28 Waters Technologies Corporation Methods of electrospray ionization of glycans modified with amphipathic, strongly basic moieties
CN109641846A (en) 2016-06-21 2019-04-16 沃特世科技公司 Fluorescent labeling of glycans and other biomolecules by reductive amination for enhanced MS signal
EP3479103B1 (en) * 2016-07-01 2022-04-20 Waters Technologies Corporation Methods for the rapid preparation of labeled glycosylamines from complex matrices using molecular weight cut off filtration and on-filter deglycosylation
EP3676393A4 (en) 2017-09-01 2021-10-13 Venn Biosciences Corporation Identification and use of glycopeptides as biomarkers for diagnosis and treatment monitoring
JP2021500539A (en) * 2017-10-18 2021-01-07 ヴェン バイオサイエンシズ コーポレーション Identification and use of biological parameters for diagnostic and therapeutic monitoring
KR102544013B1 (en) 2020-01-21 2023-06-19 리제너론 파마슈티칼스 인코포레이티드 Deglycosylation method for electrophoresis of glycosylated proteins

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5593580A (en) * 1986-11-26 1997-01-14 Kopf; Henry B. Filtration cassette article, and filter comprising same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5593580A (en) * 1986-11-26 1997-01-14 Kopf; Henry B. Filtration cassette article, and filter comprising same

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CENTRICON, MILLIPORE: "Centricon Plus-20 Centrifugal Filter devices", P31925 , REV. G, vol. 1/05, 14 March 2005 (2005-03-14), pages 1 - 2, Retrieved from the Internet <URL:http://www.millipore.com/userguides.nsf/a73664f9f981af8c852569b9005b4eee/6af1562ba38e63d385256c6b0055477f/$FILE/P31925.pdf> [retrieved on 20131022] *
CHENG ET AL.: "Identification of Two Testosterone-responsive Testicular Proteins in Sertoli Cell -enriched Culture Medium Whose Secretion Is Suppressed by Cells of the Intact Seminiferous Tubule", vol. 262, no. 26, 15 September 1987 (1987-09-15), pages 12768 - 12779 *
MANJUNATH ET AL.: "Biochemical , Biological, and Immunological Properties of Chemically Deglycosylated Human Choriogonadotropin", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 257, no. 12, 25 June 1982 (1982-06-25), pages 7109 - 7115 *
POLO ET AL.: "Crystallization and preliminary X-ray diffraction analysis of the fructofuranosidase from Xanthophyllomyces dendrorhous", ACTA CRYSTALLOGRAPHICA SECTION F STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, vol. 66, 28 October 2010 (2010-10-28), pages 1441 - 1444 *

Also Published As

Publication number Publication date
WO2013192530A2 (en) 2013-12-27

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