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WO2013188635A3 - Novel methods of identifying proteins via n-terminal tagging and selective pull-down - Google Patents

Novel methods of identifying proteins via n-terminal tagging and selective pull-down Download PDF

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Publication number
WO2013188635A3
WO2013188635A3 PCT/US2013/045594 US2013045594W WO2013188635A3 WO 2013188635 A3 WO2013188635 A3 WO 2013188635A3 US 2013045594 W US2013045594 W US 2013045594W WO 2013188635 A3 WO2013188635 A3 WO 2013188635A3
Authority
WO
WIPO (PCT)
Prior art keywords
novel methods
proteins via
identifying proteins
terminal tagging
selective pull
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2013/045594
Other languages
French (fr)
Other versions
WO2013188635A2 (en
Inventor
E. James PETERSSON
Christopher J. Noren
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
University of Pennsylvania Penn
Original Assignee
New England Biolabs Inc
University of Pennsylvania Penn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc, University of Pennsylvania Penn filed Critical New England Biolabs Inc
Publication of WO2013188635A2 publication Critical patent/WO2013188635A2/en
Anticipated expiration legal-status Critical
Publication of WO2013188635A3 publication Critical patent/WO2013188635A3/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention includes a selective method of modifying the N-terminus of a protein using an aminoacyl tRNA transferase.
PCT/US2013/045594 2012-06-14 2013-06-13 Novel methods of identifying proteins via n-terminal tagging and selective pull-down Ceased WO2013188635A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261659562P 2012-06-14 2012-06-14
US61/659,562 2012-06-14

Publications (2)

Publication Number Publication Date
WO2013188635A2 WO2013188635A2 (en) 2013-12-19
WO2013188635A3 true WO2013188635A3 (en) 2015-07-09

Family

ID=49758892

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/045594 Ceased WO2013188635A2 (en) 2012-06-14 2013-06-13 Novel methods of identifying proteins via n-terminal tagging and selective pull-down

Country Status (1)

Country Link
WO (1) WO2013188635A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104529898B (en) * 2015-01-15 2016-07-27 成都丽凯手性技术有限公司 Azepine dibenzo cyclooctyne compounds and preparation method thereof
CN116183899B (en) * 2023-04-28 2023-06-30 天津市协和医药科技集团有限公司 Preparation method of acridinium ester marked antibody preservation solution

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007103307A2 (en) * 2006-03-03 2007-09-13 California Institute Of Technology Site-specific incorporation of amino acids into molecules
WO2011136645A1 (en) * 2010-04-27 2011-11-03 Stichting Katholieke Universiteit, More Particularly Radboud University Nijmegen Fused cyclooctyne compounds and their use in metal-free click reactions
US8097425B2 (en) * 2006-03-10 2012-01-17 Tethys Bioscience, Inc. Multiplex protein fractionation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007103307A2 (en) * 2006-03-03 2007-09-13 California Institute Of Technology Site-specific incorporation of amino acids into molecules
US8097425B2 (en) * 2006-03-10 2012-01-17 Tethys Bioscience, Inc. Multiplex protein fractionation
WO2011136645A1 (en) * 2010-04-27 2011-11-03 Stichting Katholieke Universiteit, More Particularly Radboud University Nijmegen Fused cyclooctyne compounds and their use in metal-free click reactions

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GRACIET, E ET AL.: "Amino-Transferases And The N-End Rule Pathway Of Prokaryotic/Eukaryotic Specificity In A Human Pathogen.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 103, no. 9, 21 February 2006 (2006-02-21), pages 3078 - 3083 *
WAGNER, AM ET AL.: "N-Terminal Protein Modification Using Simple Aminoacyl Transferase Substrates.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 133, no. 38, 6 September 2011 (2011-09-06), pages 15139 - 15147 *

Also Published As

Publication number Publication date
WO2013188635A2 (en) 2013-12-19

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