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WO2013173774A3 - Molecular inversion probes - Google Patents

Molecular inversion probes Download PDF

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Publication number
WO2013173774A3
WO2013173774A3 PCT/US2013/041675 US2013041675W WO2013173774A3 WO 2013173774 A3 WO2013173774 A3 WO 2013173774A3 US 2013041675 W US2013041675 W US 2013041675W WO 2013173774 A3 WO2013173774 A3 WO 2013173774A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecular inversion
inversion probes
arms
mip
transcript
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2013/041675
Other languages
French (fr)
Other versions
WO2013173774A2 (en
Inventor
Philip Rolfe
Alexander SHEH
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PATHOGENICA Inc
Original Assignee
PATHOGENICA Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PATHOGENICA Inc filed Critical PATHOGENICA Inc
Publication of WO2013173774A2 publication Critical patent/WO2013173774A2/en
Publication of WO2013173774A3 publication Critical patent/WO2013173774A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Communicable Diseases (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided herein can be a method comprising forming a molecular inversion probe (MIP; a single stranded linear molecule containing two target binding arms where the arms may be separated by a backbone sequence) to selectively target nucleotide template from any source (viral, prokaryotic, and eukaryotic) to obtain information including amount of transcript and sequence data.
PCT/US2013/041675 2012-05-18 2013-05-17 Molecular inversion probes Ceased WO2013173774A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261649048P 2012-05-18 2012-05-18
US61/649,048 2012-05-18

Publications (2)

Publication Number Publication Date
WO2013173774A2 WO2013173774A2 (en) 2013-11-21
WO2013173774A3 true WO2013173774A3 (en) 2014-03-13

Family

ID=49584477

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/041675 Ceased WO2013173774A2 (en) 2012-05-18 2013-05-17 Molecular inversion probes

Country Status (1)

Country Link
WO (1) WO2013173774A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106414775A (en) * 2014-04-11 2017-02-15 宾夕法尼亚大学董事会 Compositions and methods for metagenome biomarker detection
CN104178586B (en) * 2014-09-01 2015-12-30 亚能生物技术（深圳）有限公司 The nucleic acid film bar detected for HBV somatotype and drug resistant mutant genes and test kit
US20180171386A1 (en) * 2015-06-03 2018-06-21 The General Hospital Corporation Long Adapter Single Stranded Oligonucleotide (LASSO) Probes to Capture and Clone Complex Libraries
JP7025340B2 (en) 2016-02-26 2022-02-24 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー Multiplexed single molecule RNA visualization using a two-probe proximity ligation system
WO2018194437A2 (en) * 2017-04-21 2018-10-25 이화여자대학교 산학협력단 Nucleic acid molecule template for detecting target point mutation gene and genetic analysis method using same
CN106939360B (en) * 2017-05-24 2018-04-20 贵州金域医学检验中心有限公司 PCR amplification primer, kit and the detection method of HCV 2a hypotype NS5B abrupt climatic changes
CN114807306A (en) * 2017-12-08 2022-07-29 10X基因组学有限公司 Methods and compositions for labeling cells
EP3775268A4 (en) 2018-04-09 2021-12-22 The Board Of Trustees Of The Leland Stanford Junior University PROCEDURES FOR IN SITU GENE SEQUENCING
CN111235315A (en) * 2020-03-13 2020-06-05 苏州智享众创孵化管理有限公司 Method for simultaneously detecting multiple genotypes of hepatitis E virus
CN112063690A (en) * 2020-09-18 2020-12-11 北京求臻医学检验实验室有限公司 Construction method and application of single-molecule probe multi-target capture library

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030053987A1 (en) * 1996-06-11 2003-03-20 Donnelly John J. Synthetic hepatitis c genes
US20030211467A1 (en) * 1999-12-21 2003-11-13 Schlauder George G. Methods and compositions for detecting hepatitis E virus
US20100184205A1 (en) * 2006-12-05 2010-07-22 Issac Bentwich Nucleic acids involved in viral infection
US20110059513A1 (en) * 2007-12-20 2011-03-10 Hvidovre Hospital Efficient cell culture system for hepatitis c virus genotype 1a and 1b
US20110150922A1 (en) * 2007-08-16 2011-06-23 Chrontech Pharma Ab Immunogen platform
WO2011156795A2 (en) * 2010-06-11 2011-12-15 Pathogenica, Inc. Nucleic acids for multiplex organism detection and methods of use and making the same

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030053987A1 (en) * 1996-06-11 2003-03-20 Donnelly John J. Synthetic hepatitis c genes
US20030211467A1 (en) * 1999-12-21 2003-11-13 Schlauder George G. Methods and compositions for detecting hepatitis E virus
US20100184205A1 (en) * 2006-12-05 2010-07-22 Issac Bentwich Nucleic acids involved in viral infection
US20110150922A1 (en) * 2007-08-16 2011-06-23 Chrontech Pharma Ab Immunogen platform
US20110059513A1 (en) * 2007-12-20 2011-03-10 Hvidovre Hospital Efficient cell culture system for hepatitis c virus genotype 1a and 1b
WO2011156795A2 (en) * 2010-06-11 2011-12-15 Pathogenica, Inc. Nucleic acids for multiplex organism detection and methods of use and making the same

Also Published As

Publication number Publication date
WO2013173774A2 (en) 2013-11-21

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