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WO2013165042A1 - Puce pour séparation du sang et outil de diagnostic comportant celle-ci - Google Patents

Puce pour séparation du sang et outil de diagnostic comportant celle-ci Download PDF

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Publication number
WO2013165042A1
WO2013165042A1 PCT/KR2012/003637 KR2012003637W WO2013165042A1 WO 2013165042 A1 WO2013165042 A1 WO 2013165042A1 KR 2012003637 W KR2012003637 W KR 2012003637W WO 2013165042 A1 WO2013165042 A1 WO 2013165042A1
Authority
WO
WIPO (PCT)
Prior art keywords
blood
chip
pretreatment
ultrasonic
ultrasonic vibrator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2012/003637
Other languages
English (en)
Korean (ko)
Inventor
조병호
조용호
박상희
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MICRO FRIEND CO Ltd
Original Assignee
MICRO FRIEND CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MICRO FRIEND CO Ltd filed Critical MICRO FRIEND CO Ltd
Publication of WO2013165042A1 publication Critical patent/WO2013165042A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/28—Mechanical auxiliary equipment for acceleration of sedimentation, e.g. by vibrators or the like
    • B01D21/283—Settling tanks provided with vibrators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/14—Devices for taking samples of blood ; Measuring characteristics of blood in vivo, e.g. gas concentration within the blood, pH-value of blood
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2221/00—Applications of separation devices
    • B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Definitions

  • Prostate cancer is currently the second largest cause of cancer deaths in US men, and in Korea, according to the Central Cancer Registry, prostate cancer is currently ranked fifth in the long-term men's cancer incidence rate. It is so fast that it is expected to become the most common cancer in Korea in the future.
  • the 10-year survival rate is more than 80% when it is detected early, and the treatment effect is good. We can greatly contribute to welfare policy.
  • Another object of the present invention is to provide a diagnostic cartridge that can be used in a portable diagnostic apparatus using nanobiosensor technology.
  • the present invention is a substrate; A pretreatment inflow path formed on the substrate and into which blood for inspection is introduced; An ultrasonic vibrator formed under the substrate to impart ultrasonic vibration to blood passing through the pretreatment inlet; A branching node for branching the blood flow subjected to the ultrasonic vibration by the ultrasonic vibrator; And it provides a chip for blood separation comprising a pre-treatment outflow path for outputting the branched blood.
  • the ultrasonic vibrator and the branch node may be added or subtracted according to the degree of purification of the plasma to be separated.
  • a first ultrasonic vibrator formed under the substrate and imparting ultrasonic vibration to the blood passing through the inflow path; A first branch node for branching blood flow subjected to the ultrasonic vibration by the first ultrasonic vibrator; A second ultrasonic vibrator configured to impart ultrasonic vibrations to blood flowing branched by the first branch node; And a second branch node for branching the blood flow subjected to the ultrasonic vibration by the second ultrasonic vibrator.
  • the probe molecule may be attached to the electrode through the silicon nanowires.
  • 2B is an image showing a state in which ultrasonic vibration is applied when virtual blood flows through a branch node.
  • 3A-3C illustrate a diagnostic cartridge according to another embodiment of the present invention.
  • 4B illustrates the diagnostic cartridge coupled to the reader.
  • a pretreatment chip for blood separation includes a substrate; An inflow path 120 formed on the substrate and into which blood for inspection is introduced; A first ultrasonic vibrator 130 formed below the substrate and configured to impart ultrasonic vibration to blood passing through the inflow path 120; A first branch node (140) for branching the blood flow subjected to the ultrasonic vibration by the first ultrasonic vibrator (130); A second ultrasonic vibrator 160 which imparts ultrasonic vibrations to blood flowing branched by the first branch node 140; A second branch node 170 for branching the blood flow subjected to the ultrasonic vibration by the second ultrasonic vibrator 160; And an outlet passage 182 for outputting the branched blood.
  • the first branch node 140 and / or the second branch node 170 may include one node inflow path and three branch flow paths branched from the node.
  • At least one of the placement position, vibration frequency, and vibration amplitude of the first / second ultrasonic oscillator is determined such that the blood cell component contained in the blood is driven toward the wall of the flow path by the first ultrasonic wave and / or the second ultrasonic wave.
  • the first ultrasonic wave and / or the second ultrasonic wave can be.
  • the first ultrasonic vibrator or the second ultrasonic vibrator generate ultrasonic vibration of 2 MHz.
  • the first ultrasonic vibrator 130-the first branch node 140 separates relatively large blood cells, and the second ultrasonic vibrator 160-the second branch node 170 relatively small blood.
  • the components can be separated.
  • the first branch node 140 and the second branch node 170 may form different cross-sectional areas of each sub-branch and / or angles from the main branch path.
  • first branch node 140 and the second branch node 170 may have different cross sectional area ratios between the sub branch paths and the main branch paths, and the first ultrasonic vibrator 130 and the The vibration frequency of the second ultrasonic vibrator 160 may be formed differently.
  • the number of the ultrasonic vibrator and the branch node can be increased or decreased depending on the degree of purification of the plasma to be separated.
  • 3A-3C illustrate a diagnostic cartridge according to another embodiment of the present invention.
  • the illustrated diagnostic cartridge is composed of a pre-processing chip 101 for separating the components of blood for pretreatment of blood, and a sensor chip 200 for performing tests on the separated components of blood, and is mounted on a separate reader. In the blood test may be performed, and when the blood test is completed, it may be detached from the reader.
  • tPSA- and fPSA-specific antibodies selected in the first screening process for immobilizing antibodies on the nanowires are fixed using the sol-gel method, and then the antigen-antibody reaction and the antibody are analyzed by fluorescence scanner.
  • tests for prostate disease can be performed by comparing the sensitivity and electrical signals from the used nucleic acid marker response.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Fluid Mechanics (AREA)
  • Medical Informatics (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Electrochemistry (AREA)
  • Veterinary Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Ecology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
PCT/KR2012/003637 2012-04-30 2012-05-09 Puce pour séparation du sang et outil de diagnostic comportant celle-ci Ceased WO2013165042A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020120045805A KR101391862B1 (ko) 2012-04-30 2012-04-30 혈액 분리를 위한 칩을 구비한 진단 기구
KR10-2012-0045805 2012-04-30

Publications (1)

Publication Number Publication Date
WO2013165042A1 true WO2013165042A1 (fr) 2013-11-07

Family

ID=49514433

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/003637 Ceased WO2013165042A1 (fr) 2012-04-30 2012-05-09 Puce pour séparation du sang et outil de diagnostic comportant celle-ci

Country Status (2)

Country Link
KR (1) KR101391862B1 (fr)
WO (1) WO2013165042A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10731208B2 (en) 2014-10-30 2020-08-04 EWHA University—Industry Collaboration Foundation Microfluidic device for detecting target gene, method for manufacturing same, and method for detecting using same
KR101720281B1 (ko) * 2016-02-05 2017-04-10 주식회사 아이엠헬스케어 나노 와이어를 감지 채널로 이용하고 멤브레인을 유동 채널로 이용하는 ｆｅｔ 기반 바이오 센서, 및 이를 이용한 검출 방법
WO2019103165A1 (fr) 2017-11-21 2019-05-31 주식회사 비비비 Biocapteur
KR101956860B1 (ko) * 2017-12-14 2019-03-11 포항공과대학교 산학협력단 미세유체 채널이 형성된 멤브레인을 이용한 fet 기반 다중 바이오센서 및 이를 이용한 검출 방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002072235A1 (fr) * 2001-03-09 2002-09-19 Erysave Ab Dispositif et procede de separation
KR100442838B1 (ko) * 2001-12-11 2004-08-02 삼성전자주식회사 프로브의 고정화 검출방법 및 상기 프로브와 표적시료의결합정도 검출방법
KR100518504B1 (ko) * 2002-05-29 2005-10-04 이영태 혈구 카운터
KR20090065124A (ko) * 2007-12-17 2009-06-22 한국전자통신연구원 실리콘 나노선을 이용한 바이오 센서 및 그 제조 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002072235A1 (fr) * 2001-03-09 2002-09-19 Erysave Ab Dispositif et procede de separation
KR100442838B1 (ko) * 2001-12-11 2004-08-02 삼성전자주식회사 프로브의 고정화 검출방법 및 상기 프로브와 표적시료의결합정도 검출방법
KR100518504B1 (ko) * 2002-05-29 2005-10-04 이영태 혈구 카운터
KR20090065124A (ko) * 2007-12-17 2009-06-22 한국전자통신연구원 실리콘 나노선을 이용한 바이오 센서 및 그 제조 방법

Also Published As

Publication number Publication date
KR101391862B1 (ko) 2014-05-07
KR20130122457A (ko) 2013-11-07

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