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WO2013144409A3 - Vectors for identifying hematopoietic lineage - Google Patents

Vectors for identifying hematopoietic lineage Download PDF

Info

Publication number
WO2013144409A3
WO2013144409A3 PCT/ES2013/070200 ES2013070200W WO2013144409A3 WO 2013144409 A3 WO2013144409 A3 WO 2013144409A3 ES 2013070200 W ES2013070200 W ES 2013070200W WO 2013144409 A3 WO2013144409 A3 WO 2013144409A3
Authority
WO
WIPO (PCT)
Prior art keywords
vectors
hematopoietic
hematopoietic lineage
hpcs
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/ES2013/070200
Other languages
Spanish (es)
French (fr)
Other versions
WO2013144409A2 (en
Inventor
Francisco MARTÍN MOLINA
Pilar MUÑOZ FERNÁNDEZ
Miguel GARCÍA TOSCANO
Karin BENABDELLAH
Marién COBO PULIDO
Per Anderson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Instituto de Salud Carlos III
Fundacion Publica Andaluza Progreso y Salud
Original Assignee
Instituto de Salud Carlos III
Fundacion Publica Andaluza Progreso y Salud
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto de Salud Carlos III, Fundacion Publica Andaluza Progreso y Salud filed Critical Instituto de Salud Carlos III
Publication of WO2013144409A2 publication Critical patent/WO2013144409A2/en
Publication of WO2013144409A3 publication Critical patent/WO2013144409A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to the use of specific lentiviral vectors of hematopoietic tissue, which, when under the control of the Wiskott-Aldrich syndrome (WAS) gene promoter (AWE and WE), identify novel hematopoietic progenitors (HPCs) and novel sub-populations within said progenitors derived from pluripotent stem cells. The present invention also describes the HPCs isolated using the aforementioned identification method, as well as the use thereof in the treatment of hematopoietic diseases.
PCT/ES2013/070200 2012-03-26 2013-03-26 Vectors for identifying hematopoietic lineage Ceased WO2013144409A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ESP201230449 2012-03-26
ES201230449 2012-03-26

Publications (2)

Publication Number Publication Date
WO2013144409A2 WO2013144409A2 (en) 2013-10-03
WO2013144409A3 true WO2013144409A3 (en) 2014-01-16

Family

ID=48613662

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2013/070200 Ceased WO2013144409A2 (en) 2012-03-26 2013-03-26 Vectors for identifying hematopoietic lineage

Country Status (1)

Country Link
WO (1) WO2013144409A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3880307A1 (en) * 2018-11-14 2021-09-22 Fundación Pública Andaluza Progreso Y Salud Polynucleotide for safer and more effective immunotherapies

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090028836A1 (en) * 2005-06-01 2009-01-29 Kalle Christof Von Stem and progenitor cell expansion by evi, evi-like genes and setbp1

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090028836A1 (en) * 2005-06-01 2009-01-29 Kalle Christof Von Stem and progenitor cell expansion by evi, evi-like genes and setbp1

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FRECHA C ET AL: "Improved lentiviral vectors for Wiskott-Aldrich syndrome gene therapy mimic endogenous expression profiles throughout haematopoiesis.", GENE THERAPY JUN 2008, vol. 15, no. 12, June 2008 (2008-06-01), pages 930 - 941, XP002713319, ISSN: 1476-5462 *
LEUCI VALERIA ET AL: "Efficient transcriptional targeting of human hematopoietic stem cells and blood cell lineages by lentiviral vectors containing the regulatory element of the Wiskott-Aldrich syndrome gene.", STEM CELLS (DAYTON, OHIO) NOV 2009, vol. 27, no. 11, November 2009 (2009-11-01), pages 2815 - 2823, XP002713320, ISSN: 1549-4918 *
TOSCANO MIGUEL G ET AL: "Hematopoietic-specific lentiviral vectors circumvent cellular toxicity due to ectopic expression of Wiskott-Aldrich syndrome protein.", HUMAN GENE THERAPY FEB 2008, vol. 19, no. 2, February 2008 (2008-02-01), pages 179 - 197, XP002713321, ISSN: 1043-0342 *

Also Published As

Publication number Publication date
WO2013144409A2 (en) 2013-10-03

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