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WO2013028643A8 - Preparation of polynucleotides on a solid substrate for sequencing - Google Patents

Preparation of polynucleotides on a solid substrate for sequencing Download PDF

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Publication number
WO2013028643A8
WO2013028643A8 PCT/US2012/051632 US2012051632W WO2013028643A8 WO 2013028643 A8 WO2013028643 A8 WO 2013028643A8 US 2012051632 W US2012051632 W US 2012051632W WO 2013028643 A8 WO2013028643 A8 WO 2013028643A8
Authority
WO
WIPO (PCT)
Prior art keywords
sequencing
solid substrate
preparation
polynucleotides
libraries
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/051632
Other languages
French (fr)
Other versions
WO2013028643A1 (en
Inventor
Michael Tanner
Stevan B. Jovanovich
Ezra Van Gelder
Dennis Harris
Charles Park
Seth Stern
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Integenx Inc
Original Assignee
Integenx Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2011/048528 external-priority patent/WO2012024658A2/en
Application filed by Integenx Inc filed Critical Integenx Inc
Publication of WO2013028643A1 publication Critical patent/WO2013028643A1/en
Publication of WO2013028643A8 publication Critical patent/WO2013028643A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C49/00—Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
    • C07C49/587—Unsaturated compounds containing a keto groups being part of a ring
    • C07C49/753—Unsaturated compounds containing a keto groups being part of a ring containing ether groups, groups, groups, or groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides methods and devices for performing nucleic acid amplification and sequencing on a solid substrate (e.g., a flow cell), including preparation of libraries of amplified DNA fragments for massively parallel (next-generation) sequencing.
PCT/US2012/051632 2011-08-20 2012-08-20 Preparation of polynucleotides on a solid substrate for sequencing Ceased WO2013028643A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
IBPCT/US2011/048528 2011-08-20
USPCT/US2011/048528 2011-08-20
PCT/US2011/048528 WO2012024658A2 (en) 2010-08-20 2011-08-20 Integrated analysis system
US201261602483P 2012-02-23 2012-02-23
US61/602,483 2012-02-23

Publications (2)

Publication Number Publication Date
WO2013028643A1 WO2013028643A1 (en) 2013-02-28
WO2013028643A8 true WO2013028643A8 (en) 2013-04-25

Family

ID=47746807

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/051632 Ceased WO2013028643A1 (en) 2011-08-20 2012-08-20 Preparation of polynucleotides on a solid substrate for sequencing

Country Status (1)

Country Link
WO (1) WO2013028643A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105525357A (en) * 2014-09-30 2016-04-27 深圳华大基因股份有限公司 Sequencing library construction method, and kit and application thereof

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10208332B2 (en) 2014-05-21 2019-02-19 Integenx Inc. Fluidic cartridge with valve mechanism
CA3172086A1 (en) * 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
CN107106983B (en) 2014-10-22 2021-04-16 尹特根埃克斯有限公司 Systems and methods for sample preparation, processing and analysis
US10210410B2 (en) 2014-10-22 2019-02-19 Integenx Inc. Systems and methods for biometric data collections
WO2016134521A1 (en) * 2015-02-27 2016-09-01 The University Of Hong Kong Dna display and methods thereof
US9434996B1 (en) * 2015-03-13 2016-09-06 Tracy Ann Hayden All mini-STR multiplex with increased C.E. through-put by STR prolongation template fusion
WO2016170182A1 (en) 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on a solid support
WO2016170179A1 (en) * 2015-04-24 2016-10-27 Qiagen Gmbh Method for immobilizing a nucleic acid molecule on solid support
US20190048335A1 (en) * 2016-03-17 2019-02-14 Life Technologies Corporation Improved amplification and sequencing methods
US10538796B2 (en) * 2016-10-13 2020-01-21 Agilent Technologies, Inc. On-array ligation assembly
US20190085390A1 (en) * 2017-09-18 2019-03-21 Corning Incorporated Flow cells having reactive surfaces for nucleic acid sequence analysis
WO2021067286A1 (en) * 2019-09-30 2021-04-08 Shield Diagnostics Corp. Devices for determining a target nucleic acid profile and methods of use thereof
WO2023023638A1 (en) * 2021-08-20 2023-02-23 Singular Genomics Systems, Inc. Chemical and thermal assisted nucleic acid amplification methods

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040018491A1 (en) * 2000-10-26 2004-01-29 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105525357A (en) * 2014-09-30 2016-04-27 深圳华大基因股份有限公司 Sequencing library construction method, and kit and application thereof
CN105525357B (en) * 2014-09-30 2018-08-21 深圳华大基因股份有限公司 The construction method and kit of a kind of sequencing library and application

Also Published As

Publication number Publication date
WO2013028643A1 (en) 2013-02-28

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