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WO2013024486A1 - Formulation biologique anti-termite et procédé pour la préparation de celle-ci - Google Patents

Formulation biologique anti-termite et procédé pour la préparation de celle-ci Download PDF

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Publication number
WO2013024486A1
WO2013024486A1 PCT/IN2011/000844 IN2011000844W WO2013024486A1 WO 2013024486 A1 WO2013024486 A1 WO 2013024486A1 IN 2011000844 W IN2011000844 W IN 2011000844W WO 2013024486 A1 WO2013024486 A1 WO 2013024486A1
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WO
WIPO (PCT)
Prior art keywords
formulation
termite
termites
essential oil
volume
Prior art date
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Ceased
Application number
PCT/IN2011/000844
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English (en)
Inventor
Anurag Pandey
Pronobesh CHATTOPADHYAY
Indra BARUAH
Lokendra Singh
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Director General Defence Research & Development Corp
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Director General Defence Research & Development Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Priority to PH1/2014/500365A priority Critical patent/PH12014500365B1/en
Priority to AU2011375107A priority patent/AU2011375107B2/en
Publication of WO2013024486A1 publication Critical patent/WO2013024486A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/27—Pseudomonas

Definitions

  • the present invention relates to an anti-termite biological formulation and a process for the preparation thereof.
  • an anti-termite formulation comprises a liquid or solid carrier material for the volatilizable/non-volatilizable components.
  • the carrier enables more efficient transport and distribution, such as by spreading on the surface.
  • the carrier material may also serve to depress or elevate the vapour pressure of the volatilizable component, so as to increase, moderate or sustained volatilization of that component.
  • U.S.Patent 2007/7282212 discloses a method of controlling termites comprising thiamethoxam as an active ingredient with at least one insecticide mixing partner selected from fipronil and lambda-cyhalothrin; and an adjuvant.
  • WO/2004/029017 discloses an anti-termite, waterproofing agents; binders; strengtheners; antifouling agents; antimicrobial agents; and/or biocides, which comprises of water insoluble quaternary ammonium compounds as an active ingredient.
  • Japanese Patent 2006/JP2006347894 discloses an anti-termite agent comprising 2-aryl-4-alkylimidazole, 2-phenyl-4-methylimidazole, 2- alkylimidazole, 2-methylimidazole, or the like as an active ingredient.
  • the anti-termite agents are suitable for use for treating xylem or soil.
  • the anti- termite agent can be caused to be brought into contact with termites or eaten by termites.
  • the invention relates to an aqueous composition for preserving wood and wood materials, comprising a combination of fungicides selected from the group consisting of a-[[2-(4- chlorophenyl)ethyl]-a-( 1 , 1 -dimethylethyl)- 1 H- 1 ,2,4-triazol-l-ethanol and other 1 - [ [2-(2,4-dichlorophenyl-4-propyl- 1 ,3 dioxolan-2-yl ⁇ methyl ⁇ - 1 H- 1 ,2,4-triazole, a fungicidal carbamate; namely 3-iodo-2-propynylbutyl carbamate or methyl benzimidazol-2-ylcarbamate; a stabilizer, 2, 2, 4- trimethyl-l,3-pentadiol monoisobutyrate a stabilizer and a combination of fungicides selected from the group consisting of a-[[2-(4
  • US Patent 1975/3858346 discloses the Control of subterranean termites in buildings is carried out by impregnating at least the exposed lower timbers of the building hexachlorocyclopentadiene dimmer as an active ingredient in a liquid organic solvent carrier.
  • WO/201 1/027362 discloses a herbal formulation that consists active extracts of Azadirachta indica, Pongamia pinnata and genus of Acorus, Allium, and Vitex, sesame oil infused with peel of genus Citrus, pitch, and cashew nut shell liquid -by product of cashew industry, along with suitable carrier solvents and emulsifiers, which in cumulative effect controls termites, white ants, wood borers and wood decaying fungi.
  • An object of the present invention is to provide a biological formulation for termites
  • Another object of the present invention is to provide a process for preparing biological formulation for termites
  • Still another object of the present invention is to provide a non-toxic and environment friendly formulation for termites
  • Still further object of this invention is to provide a biological formulation with superior properties
  • Still another object of this invention is to provide a process for the stable biological based anti-termite formulation.
  • a biological formulation comprising:
  • Plant and Microorganism could be an important source for development of environmentally safe and economically viable termiticide because they constitute a potential source of bioactive compounds / secondary metabolites and typically free from harmful effects.
  • the essential oil extracted from plant sources of Curcuma aromatica, aqueous seed extract of a plant Cuminum cyminum, and secondary metabolites isolated from Pseudomonas aeruginosa were found effective as termiticide and termite repellent in the present investigation.
  • the termiticide and termite repellent compositions of the invention can be used to repel and kill termites from a wood and wood objects, plants, soil, residential and commercial buildings, or other cellulosic material, and include, as active ingredients i.e.
  • C. aromatica Salisb is a perennial tuberous herb with annulate, aromatic yellow rhizome which is internally orange-red in color
  • Leaves are elliptic or lanceolate-oblong, caudate-acuminate, 30- 60cm long, petioles as long or even longer, bracts ovate, recurved, more or less tinged with red or pink.
  • Rhizomes of turmeric are used in combination with astringents and aromatics for bruises, sprains, hiccough, bronchitis, cough, leucoderma and skin eruptions.
  • Oil of Curcuma aromatica is used for treatment of early stage of cervix cancer
  • Rhizomes of Curcuma aromatica yield 6.1 % essential oil.
  • Essential oil contains a -and - b-curcumene, d-camphene and p-methoxy cinnamic acid.
  • the colouring matter is curcumin and the active constituents of oil are curcumol and curdione.
  • C. aromatica rhizomes contain ar-curcumene (18.6%), beta curcumene (25.5%) and xanthorhizol (25.7%) (Zwaving and Bos, 1992).
  • Cumin is a strong aromatic dried ripe fruit (seed) of Cuminum cyminum L. It belongs to the Apiaceae family (parsley family). The cumin plant grows to 30-50 cm (0.98-1.6 ft) tall and is harvested by hand.
  • P. aeruginosa secretes a variety of pigments, including pyocyanin (blue-green), pyoverdine (yellow-green and fluorescent), and pyorubin (red-brown) (Raney et al, 1954).
  • P. aeruginosa is often preliminarily identified by its pearlescent appearance and grape-like or tortilla-like odor in vitro. Definitive clinical identification of P.aeruginosa often includes identifying the production of both pyocyanin and fluorescein, as well as its ability to grow at 42°C. P. aeruginosa is capable of growth in diesel and jet fuel, where it is known as a hydrocarbon-using microorganism (or "HUM bug"), causing microbial corrosion. Although classified as an aerobic organism, P. aeruginosa is considered by many as a facultative anaerobe, as it is well adapted to proliferate in conditions of partial or total oxygen depletion.
  • HUM bug hydrocarbon-using microorganism
  • This organism can achieve anaerobic growth with nitrate as a terminal electron acceptor, and in its absence, it is also able to ferment arginine by substrate-level phosphorylation (Whiteley et al, 2007, Han et al, 1984).
  • Pseudomonas aeruginosa simultaneously produced secondary metabolites, 86% produced 2-alkyl-4-hydroxyquinolines, 75% produced 2-alkyl-4-hydroxyquinolines, 75% produced rhamnolipids, and 58%) produced phenazines, including pyocyanin (Hastie et al, 1984). • P. aeruginosa secretes numerous proteins and secondary metabolites (Britigan et al, 2003).
  • the present invention proposes anti-termite formulation for spray application as one of the simplest, economical, nontoxic and effective anti- termite formulation against termites.
  • the formulation compositions are more substantive to termite infected surfaces and are thus more effective than prior repellent, pesticide or termiticide product compositions.
  • the disclosed invention is a biological formulation for treating wood to preserve the appearance and condition of the treated wood from termite.
  • the formulation, as defined in the present invention also effectively controls termites in agriculture fields.
  • the present formulation avoids the problems of previously mentioned chemical pesticides, termiticide and termite repellents, which can harm the environment and are hazardous to handle, by using ingredients which are easily accessible and environmentally safe and when used in the manner described here are lethal to termites.
  • Polysorbate-80 an inert carrier in which essential oil of Curcuma aromatica, aqueous seeds extract of Cuminum cyminum and secondary metabolites isolated from Pseudomonas aeruginosa as the sole anti-termite agents are disposed.
  • the disclosed formulation may further comprise of Polysorbate- 80 as an emulsifier and nonionic surfactant.
  • the Polysorbate-80 may be used to enhance the solubility and/or stabilize the formulation, which regarded as safe for agricultural and household uses.
  • the present inventors attempted to formulate a spray based formulation of aqueous seeds extract of Cuminum cyminum, essential oil of Curcuma aromatica, and secondary metabolites isolated from Pseudomonas aeruginosa in Polysorbate-80 an acceptable inert carrier.
  • a formulation obtained by mixing essential oils of Curcuma aromatica, aqueous seeds extract of Cuminum cyminum and secondary metabolites isolated from Pseudomonas aeruginosa with Polysorbate-80 as inert carrier base has been found to have good absorbability with termite infected surfaces which is preferred in terms of stability, substantive to various surfaces, provides increased effective lifetimes with acceptable carrier.
  • a biological formulation comprising by volume of aqueous seeds extract of Cuminum cyminum, essential oil of Curcuma aromatica, and secondary metabolites isolated from Pseudomonas aeruginosa and Polysorbate-80 was used as carrier.
  • the Polysorbate-80/Tween-80 used in the formulation of this invention includes a nonionic surfactant and emulsifier derived from polyethoxylated sorbitan and oleic acid, and is often used as an emulsifier and excipient that is used to stabilize aqueous formulations, as a well regard pharmaceutically acceptable ingredients.
  • the aqueous extract of Cuminum cyminum as a major ingredient of the formulation of this invention denotes aqueous extract obtained with distilled water and grinded Cuminum cyminum seeds in a ratio of 2: 1 (water: blended Cuminum cyminum seeds) and was then left steeping for 18-24 h preferably overnight and filtered using Whatman No. 1 paper. It accounts for 10 to 60% by volume, preferably 30 to 50% by volume, more preferably 40 to 50% by volume, of the formulation.
  • the formulation of this invention may contain a pharmacologically effective substance which is essential oil of Curcuma aromatica itself proven showed antifungal and antimicrobial activity. (Asolkar et al, 1992). It accounts for 05 to 30% by volume, preferably 10 to 30% by volume, more preferably 20 to 25% by volume, of the formulation.
  • the formulation of this invention may further contain secondary metabolites isolated from Pseudomonas aeruginosa.
  • the P.aeruginosa strain identified and named DRL-1 was isoloated from 100 soil sample collected from rhizosphere region of termite infested tea plants in different locations of Assam-India using King's B medium. The fermentation was performed under fixed parameters; temperature 28°C, rpm-120, pH-7.0 for 96 hours. At the end of the fermentation the culture was centrifuged at 11, 000 rpm for about 15-20 minutes and then filtered using Whatman No. 1 paper to get the cell free filterate for further studies, which accounts for 10 to 45% by volume, preferably 10 to 30% by volume, more preferably 15 to 20% by volume, of the formulation.
  • the product may be formulated by following the steps written flow:
  • the formulation of this invention can be prepared by method For example, it can be formulated by adding essential oil of Curcuma aromatica the oil-soluble ingredients with Polysorbate-80/Tween-80, fully mixing them at a room temperature to form a component A, separately dissolving the other ingredient the aqueous seeds extract of Cuminum Cyminum and secondary metabolites isolated from Pseudomonas aeruginosa the water soluble ingredients at a room temperature to form a component B, then adding the component B in small portions to the component A with stirring at room temperature to form an intermediate emulsion, with continuing addition of the component B with stirring results in final formulation.
  • the formulation suitable for spray can be prepared by first formulating the oil and water phases separately and mixing the two together.
  • the oil phase contains about 10 to 60 percent by volume of the essential oil of Curcuma aromatica preferably added to the oil phase slowly, with stirring, at room temperature.
  • the addition of low levels of stabilizing ingredients in the water phase has been shown to be helpful.
  • the formulation of this invention has excellent storage stability of essential oil and water soluble ingredients, which proves to be effectively blending of oil in the conveying medium in turn provides long lasting effective lifetimes for the control of termites; when applied to a termite affected locus and prevent wood, wood structures, plants, soil, residential and commercial buildings, or other cellulosic material from attack of termites, with no adverse effects, easy and safe to use with acceptable carrier.
  • This invention proposes a process for preparing the highly effective biological anti-termite formulation containing essential oil of Curcuma aromatica, aqueous seeds extract of Cuminum cyminum and secondary metabolites isolated from Pseudomonas aeruginosa based formulation, which was found to give longer protection time of >08 months in field conditions against termites. Further it is nontoxic, biodegradable and safe for human use on skin of exposed body parts.
  • An anti-termite formulation in accordance with the present invention was formed as follows:
  • the formulation suitable for spray can be prepared by first formulating the oil and water phases separately and mixing the two together. It is clear from the above preparation (Formula) listed above that the biological anti-termite spray of the invention have been found improved active ingredients stability.
  • the following example illustrates the preparation of the formulation suitable for spray of this invention.
  • the individual essential oil of the plant Curcma aromatica, aqueous seed extract of Cuminum cyminum and secondary metabolites isolated from Pseudomonas aeruginosa have the application as anti-termite agents.
  • the active ingredients are tried against termites individually under laboratory conditions, as testing to determine the percentage mortality of termites on filter paper treated with individual active constituents.
  • the aqueous extract of Cuminum cyminum seeds, oil of curcuma aromatica, and secondary metabolites isolated from Pseudomonas aeruginosa exhibited some activity against targeted pest (Table 1), but the claimed product exhibited much increased mortality and knock down effect than active ingredients showed individually. This experiment confirms the existence of synergistic activity among the used active ingredients (Table 2).
  • the biological anti-termite spray based formulation for application on the surfaces of termite affected wood, wood structures, plants, soil, commercial buildings, or other cellulosic material developed in the present invention provided protection for more than 08 months in field conditions (Table 4).
  • the amount of the herbal spray formulation to be applied on termite affected surfaces varies depending upon the termite concentration. Percentage of usage may vary according to the severity of damage if already done previously. Generally, a suitable amount of it is applied to the affected surfaces two times more preferably required protection for single application only.
  • This invention proposes a process for preparing the highly effective biological anti-termite formulation containing aqueous seeds extract of Cuminum cyminum, essential oil of Curcuma aromatica, and secondary metabolites isolated from Pseudomonas aeruginosa based formulation, which was found to give longer protection time of more than 08 months in field conditions against termites in single application. Further it is nontoxic, biodegradable and safe for human use.
  • aqueous seeds extract of Cuminum cyminum 40%, essential oil of Curcuma aromatica 30%, and secondary metabolites isolated from Pseudomonas aeruginosa 20% are optimized based on the laboratory evaluation performances of individual active ingredients used in biological anti-termite formulation. At above optimized concentration exhibited the best result. There was no significant difference in the percentage mortality beyond the optimized concentration of active ingredients tested under laboratory conditions. Therefore, biological anti-termite formulation at above given concentration was taken as the standard rather than higher concentration keeping in view the cost factor of the formulation.
  • the present composition and process provides an effective anti-termite formulation contained in a unique emulsion matrix.
  • the resulting material is environmentally and cosmetically appealing, stable over time and contains no chemical active ingredients.
  • the present formulations used on surfaces of termite affected wood, wood structures, plants, soil, residential and commercial buildings, or other cellulosic material by an individual through spray to give a thin covering, in the conventional manner which has very effective anti-termite properties.
  • Table 3 Field Evaluation of Protection Time against Termite with Individual Ingredient Used In Formulation:
  • the plants are available in plenty in North Eastern region of India.
  • the essential oil is available as much as required, from the essential oil is available as much as required, from the essential oil extraction industry in NE region.
  • the essential oils are being used as insects repellent by the community since long.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Pest Control & Pesticides (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne une formulation biologique comprenant : 10 à 60 % d'un extrait aqueux de semence de Cuminum cyminum, 5-30 % d'huile essentielle de Curcuma aromatica et 10-45 % de métabolites secondaires isolés à partir de Pseudomonas aeruginosa avec au plus 12 % d'un vecteur inerte.
PCT/IN2011/000844 2011-08-18 2011-12-09 Formulation biologique anti-termite et procédé pour la préparation de celle-ci Ceased WO2013024486A1 (fr)

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PH1/2014/500365A PH12014500365B1 (en) 2011-08-18 2011-12-09 An anti-termite biological formulation and a process for the preparation thereof
AU2011375107A AU2011375107B2 (en) 2011-08-18 2011-12-09 An anti-termite biological formulation and a process for the preparation thereof

Applications Claiming Priority (2)

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IN2348/DEL/2011 2011-08-18
IN2348DE2011 2011-08-18

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AU (1) AU2011375107B2 (fr)
MY (1) MY163342A (fr)
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019157422A1 (fr) * 2018-02-09 2019-08-15 Cornell University Compositions et procédés de lutte contre les insectes
CN116287011A (zh) * 2023-02-22 2023-06-23 杭州佳嘉乐生物技术有限公司 一种黄精与琼式不动杆菌复合发酵高产表面活性剂的制备工艺

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US5089483A (en) 1988-07-22 1992-02-18 Daicel Chemical Industries Ltd. Agent for preservation of timber against decay and termite damage
US5196407A (en) 1990-05-23 1993-03-23 Desowag Materialschutz Gmbh Composition for preserving wood and wood materials
WO1999018789A1 (fr) * 1997-10-14 1999-04-22 The Board Of Regents, The University Of Texas System Formulations et methodes de lutte contre les insectes
US20030082244A1 (en) * 1998-08-21 2003-05-01 Shinji Yoshida Insecticide
WO2004029017A1 (fr) 2002-09-26 2004-04-08 Cooee Biosciences Limited Nouveaux composes d'ammonium quaternaire
US20060134341A1 (en) * 2004-12-17 2006-06-22 David Glassel Protection of construction materials from pests
JP2006347894A (ja) 2005-06-13 2006-12-28 Japan Enviro Chemicals Ltd 抗シロアリ剤
US7282212B2 (en) 2000-08-28 2007-10-16 Syngenta Crop Protection, Inc. Control of wood-destroying pests with thiamethoxam
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Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3858346A (en) 1973-01-29 1975-01-07 Allied Chem Control of subterranean termites
US5089483A (en) 1988-07-22 1992-02-18 Daicel Chemical Industries Ltd. Agent for preservation of timber against decay and termite damage
US5196407A (en) 1990-05-23 1993-03-23 Desowag Materialschutz Gmbh Composition for preserving wood and wood materials
WO1999018789A1 (fr) * 1997-10-14 1999-04-22 The Board Of Regents, The University Of Texas System Formulations et methodes de lutte contre les insectes
US20030082244A1 (en) * 1998-08-21 2003-05-01 Shinji Yoshida Insecticide
US7282212B2 (en) 2000-08-28 2007-10-16 Syngenta Crop Protection, Inc. Control of wood-destroying pests with thiamethoxam
WO2004029017A1 (fr) 2002-09-26 2004-04-08 Cooee Biosciences Limited Nouveaux composes d'ammonium quaternaire
US20060134341A1 (en) * 2004-12-17 2006-06-22 David Glassel Protection of construction materials from pests
JP2006347894A (ja) 2005-06-13 2006-12-28 Japan Enviro Chemicals Ltd 抗シロアリ剤
WO2011027362A2 (fr) 2009-09-01 2011-03-10 Vishal Kumar Préparation anti-termite à base de plantes

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019157422A1 (fr) * 2018-02-09 2019-08-15 Cornell University Compositions et procédés de lutte contre les insectes
US12114663B2 (en) 2018-02-09 2024-10-15 Cornell University Compositions and methods for insect control
CN116287011A (zh) * 2023-02-22 2023-06-23 杭州佳嘉乐生物技术有限公司 一种黄精与琼式不动杆菌复合发酵高产表面活性剂的制备工艺

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