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WO2013016418A3 - Ion channel probes and methods of use thereof - Google Patents

Ion channel probes and methods of use thereof Download PDF

Info

Publication number
WO2013016418A3
WO2013016418A3 PCT/US2012/048135 US2012048135W WO2013016418A3 WO 2013016418 A3 WO2013016418 A3 WO 2013016418A3 US 2012048135 W US2012048135 W US 2012048135W WO 2013016418 A3 WO2013016418 A3 WO 2013016418A3
Authority
WO
WIPO (PCT)
Prior art keywords
ion channel
methods
constructs
probes
channel probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/048135
Other languages
French (fr)
Other versions
WO2013016418A2 (en
Inventor
Jeffrey R. MARTENS
Sarah Marie BASS
Lian Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Michigan System
University of Michigan Ann Arbor
Original Assignee
University of Michigan System
University of Michigan Ann Arbor
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Michigan System, University of Michigan Ann Arbor filed Critical University of Michigan System
Publication of WO2013016418A2 publication Critical patent/WO2013016418A2/en
Publication of WO2013016418A3 publication Critical patent/WO2013016418A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

The disclosure relates to ion channel constructs and methods for using these ion channel constructs as probes for visualizing and monitoring the movement of ion channel subunits. Such constructs and methods provide new discovery tools for understanding how various molecules affect ion channel assembly and function and provide means for the development of new therapies directed at the regulation of ion channels.
PCT/US2012/048135 2011-07-27 2012-07-25 Ion channel probes and methods of use thereof Ceased WO2013016418A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161512257P 2011-07-27 2011-07-27
US61/512,257 2011-07-27

Publications (2)

Publication Number Publication Date
WO2013016418A2 WO2013016418A2 (en) 2013-01-31
WO2013016418A3 true WO2013016418A3 (en) 2013-06-13

Family

ID=46614639

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/048135 Ceased WO2013016418A2 (en) 2011-07-27 2012-07-25 Ion channel probes and methods of use thereof

Country Status (1)

Country Link
WO (1) WO2013016418A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014145554A2 (en) * 2013-03-15 2014-09-18 Tota Michael R Novel green fluorescent protein (gfp) peptides
US10640766B2 (en) 2017-04-12 2020-05-05 Tempo Bioscience, Inc. Biosensors for chloride ions
US10571480B2 (en) * 2017-04-12 2020-02-25 Tempo Bioscience, Inc. Biosensors for cellular osmolarity
CN111538727B (en) * 2020-04-16 2023-01-13 四川轻化工大学 Multi-path fast searching and classifying method and system based on Bloom Filter
BR112023024275A2 (en) * 2021-05-20 2024-01-30 Artema Therapeutics Inc KCNV2 VARIANTS AND THEIR USE
EP4347633A1 (en) * 2021-05-30 2024-04-10 Genentech, Inc. Mutant human nax proteins and screening methods

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050014202A1 (en) * 2003-07-15 2005-01-20 Brown Arthur M. High throughput assay systems and methods for identifying agents that alter surface expression of integral membrane proteins
WO2005013666A2 (en) * 2003-08-08 2005-02-17 Garvan Institute Of Medical Research Novel translocation assay
US20050063989A1 (en) * 2003-09-22 2005-03-24 Commissariat A L'energie Atomique Ion channel hybrid proteins as electrical sensors and use thereof
US20050191710A1 (en) * 2004-03-01 2005-09-01 Hanrahan John W. Method for labeling a membrane-localized protein
WO2006050257A2 (en) * 2004-10-29 2006-05-11 Massachusetts Institute Of Tecchnology Detection of ion channel or receptor activity
WO2006069331A2 (en) * 2004-12-22 2006-06-29 The Salk Institute For Biological Studies Compositions and methods for producing recombinant proteins
WO2009150215A2 (en) * 2008-06-12 2009-12-17 Universite Joseph Fourier Hybrid proteins comprising membrane receptor and ion channel, and their use as biosensors

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050014202A1 (en) * 2003-07-15 2005-01-20 Brown Arthur M. High throughput assay systems and methods for identifying agents that alter surface expression of integral membrane proteins
WO2005013666A2 (en) * 2003-08-08 2005-02-17 Garvan Institute Of Medical Research Novel translocation assay
US20050063989A1 (en) * 2003-09-22 2005-03-24 Commissariat A L'energie Atomique Ion channel hybrid proteins as electrical sensors and use thereof
US20050191710A1 (en) * 2004-03-01 2005-09-01 Hanrahan John W. Method for labeling a membrane-localized protein
WO2006050257A2 (en) * 2004-10-29 2006-05-11 Massachusetts Institute Of Tecchnology Detection of ion channel or receptor activity
WO2006069331A2 (en) * 2004-12-22 2006-06-29 The Salk Institute For Biological Studies Compositions and methods for producing recombinant proteins
WO2009150215A2 (en) * 2008-06-12 2009-12-17 Universite Joseph Fourier Hybrid proteins comprising membrane receptor and ion channel, and their use as biosensors

Also Published As

Publication number Publication date
WO2013016418A2 (en) 2013-01-31

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