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WO2013005224A1 - Dispositif de commande d'une pipette - Google Patents

Dispositif de commande d'une pipette Download PDF

Info

Publication number
WO2013005224A1
WO2013005224A1 PCT/IN2011/000663 IN2011000663W WO2013005224A1 WO 2013005224 A1 WO2013005224 A1 WO 2013005224A1 IN 2011000663 W IN2011000663 W IN 2011000663W WO 2013005224 A1 WO2013005224 A1 WO 2013005224A1
Authority
WO
WIPO (PCT)
Prior art keywords
speed
pipette
aspiration
liquid
dispensation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IN2011/000663
Other languages
English (en)
Other versions
WO2013005224A8 (fr
Inventor
Chirag Narendrabhai SHAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2013005224A1 publication Critical patent/WO2013005224A1/fr
Publication of WO2013005224A8 publication Critical patent/WO2013005224A8/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids

Definitions

  • the present disclosure relates to the field of pipetting. More particularly, the disclosure relates to an apparatus for aspirating liquids into and dispensing liquids from a pipette. More particularly, the present disclosure relates to a pipette controller for facilitating accurate pipetting of liquids.
  • US Pat. No. 3,963,061 discloses an apparatus for drawing liquids into and expelling liquids from a pipette including a housing comprising a hand-held pistol grip to which is connected a pipette-supporting portion. Valve means mounted on the pistol grip beneath two fingers of the user permit selective application of pressure or vacuum to one end of the pipette for drawing the liquid into, and expelling the liquid from, the pipette.
  • the patent discloses a pipette gun having an adjustable valve for continuously variably controlling the pressure applied to the pipette from a constant pressure source and, thus, continuously variably controlling the liquid flow rate through the pipette.
  • the operator controls the liquid flow rate through the pipette by limiting the extent to which the trigger on the gun is depressed. In this manner the operator can rapidly fill or void a major portion of the pipette by depressing the trigger fully and then slowly meter the pipette by depressing the trigger slightly.
  • the full range of liquid flow rates is achieved over the action path of the trigger. Due to the wide range of liquid flow rates and the limited action path of the trigger, however, slight deflections of the trigger produce significant changes in the liquid flow rate. Limiting the range of flow rates for a particular trigger path of action makes the trigger less sensitive but also limits the usefulness and efficiency of the pipette gun.
  • U.S. Pat. No. 4,527,437 discloses a pipette controller for facilitating the handling of liquids by a pipette.
  • the pipette attaches to the pipette controller.
  • the pipette controller has a handle which is proximate to the attached pipette and substantially parallel to the pipette.
  • the user operates the pipette controller by working a thumb wheel on the handle which is mechanically coupled to a bellows which controls the liquid level in the attached pipette.
  • the user grasps the hand below the thumb wheel at a position which is approximately medial to the length of a typical pipette.
  • U.S. Pat. No. 5,294,405 discloses a valve for use in a pipette gun for selectively providing different ranges of flow rates through a pipette cooperating with the pipette gun.
  • the valve has a predetermined number of discrete settings, each of which provides a range of fluid flow rates between zero and a different maximum flow rate.
  • the operator controls the flow rate by depressing the gun trigger a selected distance. To change the flow rate setting, the operator simply rotates the gun trigger to a. desired setting.
  • the present valve also eliminates the "piston effect" of prior art valve elements by isolating the pipette chamber while the valve element operably slides in and out of engagement with a port thereby connecting the pipette with a fluid source either under pressure or at a vacuum.
  • This patent mainly provides a valve for use in a pipette gun having several range settings for variably controlling the flow of liquid samples through a pipette.
  • the gun has separate triggers for activating the flow of negative and positive pressure to the pipette.
  • the pipet guns disclosed in this US patent facilitates pipetting at a variety of flow rates but cannot effectively be adjusted from one general flow rate to another during expulsion of the liquid from the pipet, due to the risk of leakage and thus, can result in inaccurate pipetting.
  • valve attempts to close off the chamber from venting to ambient air over the entire path of the trigger, an effective airtight seal is not established, since the valve and valve chamber are typically constructed of plastic materials, thus decreasing the efficiency of the pipet gun. Additionally, while progressively increasing flow rates over the path of the trigger are useful, there is no indication for the operator to determine when the flow rate increases beyond a desired flow rate threshold. Hence in summary in the pipette gun disclosed in thus US patent it is difficult to achieve exact volume of liquid in the pipette.
  • U.S. Pat. No. 6,253,628 discloses a pipette gun for drawing liquids into and dispensing liquids from a pipette which includes a variable flow valve assembly.
  • the valve assembly includes a valve which is capable of accommodating a plurality of flow rates, and which is progressively adjustable during pipetting between a first air flow rate and a second air flow rate which is in excess of the first flow rate and which is non-progressively adjustable during pipetting to a third air flow rate which is substantially greater than the second air flow rate.
  • the valve assembly further includes a threshold detector in the form of a spring detent, which provides an indication to the operator that the pipet gun is operating at the increased fluid rate, i.e., the "express mode" of operation.
  • US Pat. No. 6,090,348 discloses a method for programming an electronic pipetter to provide more precise measurement and dispensing is provided, as well as an improved display for displaying clearly and in details the programming steps and options to the user thereof.
  • the electronic pipetter may be operated in one of five modes, including a manual mode, a quick-shot mode, a multi-dispense mode, a sequential-dispense mode and a hands-free mode.
  • This patent provides electronic pipette having an advanced programming methodology and an easy-to-understand display so that the user can more exactly and quickly control the electronic pipetter, which in turn provides more precise, repeatable measuring and dispensing of fluids.
  • programming is the only way to achieve exact volume of the liquid. It is time consuming and not user friendly to program for such simple application.
  • the present invention provides an apparatus for aspirating liquids into and dispensing liquids from a pipette, wherein the apparatus comprises of a pipette connecting means adapted to operatively connect the pipette to the apparatus; a dispensation knob for dispensing liquid from the pipette at a first defined speed; an aspiration knob for aspiring liquid into the pipette at a second defined speed; and a control switch for controlling speed of aspiration and dispensation of the liquid, wherein the control switch changes speed of aspiration and dispensation from the first defined speed and the second defined speed to a minimum speed.
  • FIG. 1 illustrates a pipette controller for dispensing liquids from and aspirating liquids into a pipette according to an embodiment of the present invention.
  • Fig. 2 illustrates the pipette controller in liquid dispensing mode.
  • FIG. 3 illustrates the pipette controller in liquid aspiration mode.
  • Fig. 1 illustrates a pipette controller (1) for dispensing liquids from and aspirating liquids into a pipette according to an embodiment of the present invention.
  • the pipette controller (1) includes a micro pump (15) having a motor, a control unit (20), dispensation and aspiration knobs (4, 5) for dispensing and aspirating liquids respectively, a speed selection switch (6) for selecting a speed at which aspiration and dispensing operation will take place, and a control switch (1 1).
  • the pipette controller (1) includes a nozzle (3), a safety valve (7), and a silicon adapter (8).
  • the silicon adaptor (8) can provide a tight fit for glass and plastic pipettes.
  • the silicon adaptor (8) can be accommodated in nozzle (3).
  • the silicon adaptor (8) can be spiral shaped and can connect the pipette to the safety valve (7) and help in aspiration and dispensation of the liquid by piston cylinder type mechanism.
  • a piston (22) can be used along with an O ring (17) to carry out the aspiration and dispensation function.
  • the piston (22) and O ring (17) are shown as parts both the aspiration (5) and dispensation knob (4).
  • the safety valve (7) can be used to stop liquid from entering into the nozzle (3) so that the control unit (20) is not affected.
  • the safety valve (7) can further block the liquid from entering inside the pipette controller 1 in case of overpipetting.
  • the pipetting apparatus can be also equipped with a membrane filter (not shown) which is replaceable. The filter can protect the pipetting apparatus and the user from contamination by vapors of the liquids being drawn in the pipette.
  • the filter can further be accommodated by a filter holder (19).
  • the pipette controller (1) can operate in two modes, namely the dispensing mode and the aspiration mode.
  • the modes are selected by using push buttons (4, 5).
  • Push button (4) is provided for carrying out operation of dispensing liquid from pipette while push button (5) is provided for carrying out operation of aspiration of liquid into the pipette.
  • both push buttons (4, 5) are connected to the micro pump (15) to allow motor of the micro pump (15) to operatively function based on finger pressure on the aspiration and dispensation knob (4,5).
  • the push buttons and/or the knobs 4, 5 are independent of the micro pump (15) for their respective functions.
  • the push buttons (4, 5) can include a piston cylinder mechanism (17 and 22).
  • the pistons can move smoothly and can be operated with minimum efforts making it easy to adjust meniscus precisely at the desired point.
  • the push buttons (4, 5) can be operatively connected to the control unit (20), also interchangeably referred to as electronic control unit (20).
  • the control unit (20) can further be operative connected to the speed selection switch (6), which decides the speed at which aspiration and the dispensation of liquid would take place.
  • the speed of aspiration and dispensing can be controlled mechanically by changing the finger pressure on the push buttons (4, 5).
  • speed of the motor of micro pump (15) does not change and the motor continues to work at the same speed which is set by the speed selection switch (6).
  • Speed of aspiration and dispensing in such a case, only changes by the variation in finger pressure.
  • the push buttons (4, 5) are connected to the micro pump (15) to allow motor of the micro pump (15) to operatively function based on finger pressure on the aspiration and dispensation knob (4,5).
  • speed selection switch (6) can be used for controlling the speed of aspiration and dispensing.
  • a user can set the desired speed using the speed selection switch (6), which is operatively connected to the micro pump (15) motor through the control unit (20).
  • the electronic control unit (20) may be an electronic circuit, microprocessor or a programmable chip.
  • the main function of the electronic control unit (20) is to manage the overall operation of the pipette controller 1.
  • the present disclosure provides a motor control switch (11), also is interchangeably referred to as a control switch (11).
  • the control switch (11) allows the user to change the speed of the motor (aspiration speed or dispensation speed) from existing speed to a minimum speed (drop wise aspiration and dispensation). In an embodiment, this speed conversion can happen immediately by pressing of the control switch (1 1) once.
  • control switch (11) further allows speed change from the minimum speed to a preselected/predefined speed without stopping aspiration-dispensing.
  • control switch (1 1) can also change the speed from the minimum speed to the aspiration/dispensation speed from which it was changed earlier to the minimum speed.
  • control switch (11) can be operatively connected to the control unit (20) for controlling the operation of the motor of the micro pump (15).
  • motor of the micro pump runs at the adjusted speed / highest speed decided by the user.
  • pump (15) starts running at the slowest/minimum programmed speed.
  • this speed is already set/ programmed in the electronic control unit (20) which allows drop wise dispensing of the liquid.
  • the minimum speed can also be configurable by the user at ran time.
  • a battery charging plug (10) can be provided for charging batteries whenever required.
  • the pipette controller (1) can include an LCD (21) coupled with a Battery Cell (18) for improved display of the pipette controller 1 and parts thereof.
  • Fig. 2 and 3 illustrate the liquid dispensing 200 and aspiration mode 300 respectively.
  • a user can connect a pipette (14) to a pipette controller (1) using a pipette connector.
  • the user can then select an operation to be performed and select a speed at which it is to be performed using the speed selection switch (6).
  • the user can trigger push buttons (4, 5).
  • the user can control the speed of motor of the micropump (15) by the electronic control unit (20) or by changing the finger pressure on the push buttons (4, 5) to ensure dispensing from or aspiration into the pipette of the desired quantity of liquid.
  • the electronic control unit (20) is operatively connected to the speed selection switch (6). In yet another embodiment, the electronic control unit (20) is operatively connected to the speed selection switch (6) and/or the control switch (1 1).
  • the user can also change the speed of motor from existing speed to lowest/minimum speed once he/she aspirates liquid to a level from where desired/required level is close and from lowest speed to a predefine speed/or earlier aspiration/dispensation speed using motor control switch (11) once desired volume is achieved.
  • the apparatus can operate in two modes, namely the aspiration mode and the dispense mode. When a user presses the push button (5), motor of the micro pump (15) starts running according the electronically adjusted aspiration speed.
  • the liquid gets aspirated in the pipette (14) and remains intact after releasing the button.
  • the motor starts running according the electronically adjusted dispensing speed. In this mode the liquid gets dispensed from the pipette.
  • the present disclosure provides a pipette controller which is free from all problems and deficiencies associated with existing pipette controllers.
  • the present disclosure provides an apparatus for aspiration and dispensation of liquid in a pipette that facilitates volumetric accuracy of pipetting of liquids.
  • the present disclosure provides an apparatus for aspirating liquids into and dispensing liquids from a pipette that provides better sensitivity and accuracy even in low volume of the liquid.
  • the present disclosure provides an apparatus for aspirating liquids into and dispensing liquids from a pipette which is time efficient.
  • the present disclosure provides an apparatus for aspirating liquids into and dispensing liquids from a pipette that provides maximum working efficiency and performance even with large pipetting volumes.
  • the present disclosure provides an apparatus for aspirating liquids into and dispensing liquids from a pipette that is light in weight and simple in construction.
  • the present disclosure provides an apparatus for aspirating liquids into and dispensing liquids from a pipette that is fully user controlled and easy to handle.
  • the present disclosure provides an apparatus for aspirating liquids into and dispensing liquids from a pipette that is having a controlled speed operation.
  • the present disclosure provides an apparatus for aspirating liquids into and dispensing liquids from a pipette that can work with both glass as well as plastic pipettes.
  • the present apparatus works with all types of glass and plastic pipettes in the range of 1 ml to 200 ml.
  • the present apparatus allows changing the speed of motor from low to high and vice versa without stopping aspiration-dispensing operations.
  • Control switch, of the present apparatus, along with aspiration- dispensing knob ensures fast dispensing of large volumes and precise measuring of small volumes.
  • the present apparatus is equipped with replaceable membrane filters that protect cross contamination.
  • the filter also prevents contamination of the mechanism by reagent vapors.
  • the present apparatus allows a relaxed grip design having low weight and low resistance operation to minimize strain from prolonged pipetting.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un appareil pour aspirer du liquide dans et distribuer du liquide à partir d'une pipette (14). L'appareil se compose d'un moyen de liaison à une pipette, apte à relier de façon fonctionnelle la pipette (14) à l'appareil, un bouton de distribution (4) pour distribuer le liquide à partir de la pipette (14) à une première vitesse définie, un bouton d'aspiration (5) pour aspirer le liquide dans la pipette (14) à une seconde vitesse définie et un commutateur de commande (11) pour commander la vitesse d'aspiration ou de distribution du liquide, une pression du commutateur de commande (11) changeant la vitesse d'aspiration ou de distribution de la première vitesse définie ou de la seconde vitesse définie à une vitesse minimale.
PCT/IN2011/000663 2011-07-06 2011-09-23 Dispositif de commande d'une pipette Ceased WO2013005224A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1949/MUM/2011 2011-07-06
IN1949MU2011 2011-07-06

Publications (2)

Publication Number Publication Date
WO2013005224A1 true WO2013005224A1 (fr) 2013-01-10
WO2013005224A8 WO2013005224A8 (fr) 2013-01-31

Family

ID=47436619

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2011/000663 Ceased WO2013005224A1 (fr) 2011-07-06 2011-09-23 Dispositif de commande d'une pipette

Country Status (1)

Country Link
WO (1) WO2013005224A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114682318A (zh) * 2020-12-31 2022-07-01 苏州赛尼特格尔实验室科技有限公司 一种机械移液器

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3963061A (en) * 1975-09-16 1976-06-15 Drummond Scientific Company Apparatus for drawing liquids into, and expelling liquids from a pipette
US5294405A (en) * 1992-04-09 1994-03-15 Drummond Scientific Company Adjustable valve for pipette gun
US6253628B1 (en) * 1998-08-21 2001-07-03 Becton Dickinson And Company Apparatus for drawing liquids into and expelling liquids from a pipet at variable flow rates
US20010055547A1 (en) * 1999-03-05 2001-12-27 Rainin Instrument Co., Inc. Bilaterally symmetrical battery powered microprocessor controlled lightweight hand-holdable electronic pipette
US20020005075A1 (en) * 2000-06-26 2002-01-17 Kriz Jeffrey W. Hand-held pipette

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3963061A (en) * 1975-09-16 1976-06-15 Drummond Scientific Company Apparatus for drawing liquids into, and expelling liquids from a pipette
US5294405A (en) * 1992-04-09 1994-03-15 Drummond Scientific Company Adjustable valve for pipette gun
US6253628B1 (en) * 1998-08-21 2001-07-03 Becton Dickinson And Company Apparatus for drawing liquids into and expelling liquids from a pipet at variable flow rates
US20010055547A1 (en) * 1999-03-05 2001-12-27 Rainin Instrument Co., Inc. Bilaterally symmetrical battery powered microprocessor controlled lightweight hand-holdable electronic pipette
US20020005075A1 (en) * 2000-06-26 2002-01-17 Kriz Jeffrey W. Hand-held pipette

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114682318A (zh) * 2020-12-31 2022-07-01 苏州赛尼特格尔实验室科技有限公司 一种机械移液器

Also Published As

Publication number Publication date
WO2013005224A8 (fr) 2013-01-31

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