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WO2013095137A3 - Procédés, moyens et coffrets pour détecter des analytes peu abondants - Google Patents

Procédés, moyens et coffrets pour détecter des analytes peu abondants Download PDF

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Publication number
WO2013095137A3
WO2013095137A3 PCT/NL2012/050910 NL2012050910W WO2013095137A3 WO 2013095137 A3 WO2013095137 A3 WO 2013095137A3 NL 2012050910 W NL2012050910 W NL 2012050910W WO 2013095137 A3 WO2013095137 A3 WO 2013095137A3
Authority
WO
WIPO (PCT)
Prior art keywords
catalytic
target molecule
ligand
detecting
kits
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/NL2012/050910
Other languages
English (en)
Other versions
WO2013095137A2 (fr
Inventor
Andreas Herrmann
Deepak Kumar PRUSTY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rijksuniversiteit Groningen
Original Assignee
Rijksuniversiteit Groningen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rijksuniversiteit Groningen filed Critical Rijksuniversiteit Groningen
Publication of WO2013095137A2 publication Critical patent/WO2013095137A2/fr
Publication of WO2013095137A3 publication Critical patent/WO2013095137A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés, des moyens et des coffrets pour la détection et l'analyse quantitative d'analytes chimiques et biologique à l'aide d'une amplification de signal catalytique. L'invention concerne des procédés, des coffrets et des réactifs pour détecter une molécule cible ou faire intéragir des molécules cibles dans un échantillon sur la base d'une amplification de signal catalytique, comprenant les étapes consistant à (i) se procurer au moins une première sonde non fluorescente et une seconde sonde non fluorescente aptes à se lier à des sites de liaison distincts sur la (les) molécule(s) cible(s), la première sonde étant dotée d'un premier ligand et la seconde sonde étant dotée d'un second ligand, les premier et second ligands pouvant former un complexe catalytique en présence d'un composé catalytique et le complexe catalytique pouvant catalyser une réaction chromogène; (ii) mettre en contact un échantillon que l'on sait ou que l'on suspecte de contenir la (les molécule(s) cible(s) avec lesdites au moins première et seconde sondes non fluorescentes dans des conditions qui permettent la liaison des sondes à (aux) molécule(s) cible(s) de telle sorte que, lors de la liaison des sondes à (aux) molécule(s) cible(s), les premier et second ligands sont amenés en proximité proche l'un de l'autre et conjointement avec le composé catalytique forment un complexe catalytique immobilisé sur la (les) molécule(s) cible(s); et (iii) détecter la formation du complexe catalytique sur la base d'une conversion non enzymatique d'un colorant précurseur soluble dans l'eau en un colorant rapporteur chromophore.
PCT/NL2012/050910 2011-12-23 2012-12-21 Procédés, moyens et coffrets pour détecter des analytes peu abondants Ceased WO2013095137A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP11195613.2 2011-12-23
EP11195613 2011-12-23
US201261584824P 2012-01-10 2012-01-10
US61/584,824 2012-01-10

Publications (2)

Publication Number Publication Date
WO2013095137A2 WO2013095137A2 (fr) 2013-06-27
WO2013095137A3 true WO2013095137A3 (fr) 2013-11-07

Family

ID=48669669

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2012/050910 Ceased WO2013095137A2 (fr) 2011-12-23 2012-12-21 Procédés, moyens et coffrets pour détecter des analytes peu abondants

Country Status (1)

Country Link
WO (1) WO2013095137A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3172569A1 (fr) 2014-07-25 2017-05-31 Selux Diagnostics Inc. Procédés d'analyse impliquant des nanoparticules dissociables
WO2017196985A1 (fr) 2016-05-10 2017-11-16 Promega Corporation Catalyseurs au palladium à performances améliorées dans des environnements biologiques
JP7208219B2 (ja) 2018-03-07 2023-01-18 テルモ株式会社 生体試料中の分析対象物濃度を測定するための、方法、試薬およびチップ
CN110128455B (zh) * 2019-05-10 2021-10-15 南京林业大学 一种冰片酯基苯乙炔基BODIPY类pH荧光探针的制备方法
CN117088916A (zh) * 2022-05-11 2023-11-21 中国科学院上海有机化学研究所 用于检测痕量待测物质的含氟探针及其制备和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002033396A1 (fr) * 2000-10-14 2002-04-25 Saunders Alex M Dosage en solution faisant intervenir un ligand pour analysats a faible concentration

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002033396A1 (fr) * 2000-10-14 2002-04-25 Saunders Alex M Dosage en solution faisant intervenir un ligand pour analysats a faible concentration

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEN ET AL: "A practical palladium catalyzed dehalogenation of aryl halides and alpha-haloketones", TETRAHEDRON, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 63, no. 20, 10 April 2007 (2007-04-10), pages 4266 - 4270, XP022025055, ISSN: 0040-4020, DOI: 10.1016/J.TET.2007.03.061 *
DEEPAK K. PRUSTY ET AL: "A Fluorogenic Reaction Based on Heavy-Atom Removal for Ultrasensitive DNA Detection", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 132, no. 35, 8 September 2010 (2010-09-08), pages 12197 - 12199, XP055026940, ISSN: 0002-7863, DOI: 10.1021/ja105181v *
SHILEI ZHU ET AL: "Highly Water-Soluble Neutral BODIPY Dyes with Controllable Fluorescence Quantum Yields", ORGANIC LETTERS, vol. 13, no. 3, 4 February 2011 (2011-02-04), pages 438 - 441, XP055026854, ISSN: 1523-7060, DOI: 10.1021/ol102758z *

Also Published As

Publication number Publication date
WO2013095137A2 (fr) 2013-06-27

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