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WO2013076258A3 - Mutants p450 bm3 et leur utilisation pour l'hydroxylation régio-et stéréosélective d'αlpha- et βeta-ionone - Google Patents

Mutants p450 bm3 et leur utilisation pour l'hydroxylation régio-et stéréosélective d'αlpha- et βeta-ionone Download PDF

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Publication number
WO2013076258A3
WO2013076258A3 PCT/EP2012/073490 EP2012073490W WO2013076258A3 WO 2013076258 A3 WO2013076258 A3 WO 2013076258A3 EP 2012073490 W EP2012073490 W EP 2012073490W WO 2013076258 A3 WO2013076258 A3 WO 2013076258A3
Authority
WO
WIPO (PCT)
Prior art keywords
hydroxylation
regio
ionone
mutants
beta
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2012/073490
Other languages
English (en)
Other versions
WO2013076258A4 (fr
WO2013076258A2 (fr
Inventor
Jan COMMANDEUR
M. Venkataraman H.
Nico VERMEULEN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of WO2013076258A2 publication Critical patent/WO2013076258A2/fr
Publication of WO2013076258A3 publication Critical patent/WO2013076258A3/fr
Publication of WO2013076258A4 publication Critical patent/WO2013076258A4/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/14—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen (1.14.14)
    • C12Y114/14001—Unspecific monooxygenase (1.14.14.1)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'hydroxylation sélective est une réaction critique dans la synthèse de produits chimiques fins tels que les isoprénoïdes hydroxylés. La présente invention concerne des mutants P450 BM3 de cytochromes pouvant exécuter une hydroxylation régio- et stéréosélective d'un α- et β-ionone. L'invention concerne des polypeptides isolés ayant une activité oxydative et des polynucléotides isolés codant pour ces polypeptides. La présente invention concerne également des constructions d'acides nucléiques, des vecteurs et des cellules hôtes incluant les polynucléotides, ainsi que des procédés permettant l'hydroxylation microbiologique d'isoprénoïdes, en particulier des ionones.
PCT/EP2012/073490 2011-11-23 2012-11-23 Mutants p450 bm3 et leur utilisation pour l'hydroxylation régio-et stéréosélective d'αlpha- et βeta-ionone Ceased WO2013076258A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP11190236.7 2011-11-23
EP11190236 2011-11-23
EP11194955 2011-12-21
EP11194955.8 2011-12-21

Publications (3)

Publication Number Publication Date
WO2013076258A2 WO2013076258A2 (fr) 2013-05-30
WO2013076258A3 true WO2013076258A3 (fr) 2013-08-29
WO2013076258A4 WO2013076258A4 (fr) 2013-10-24

Family

ID=47290937

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2012/073490 Ceased WO2013076258A2 (fr) 2011-11-23 2012-11-23 Mutants p450 bm3 et leur utilisation pour l'hydroxylation régio-et stéréosélective d'αlpha- et βeta-ionone

Country Status (1)

Country Link
WO (1) WO2013076258A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108271374B (zh) * 2015-07-07 2021-09-21 科德克希思公司 具有改进的活性的新颖p450-bm3变体
WO2019152679A1 (fr) * 2018-01-31 2019-08-08 The Regents Of The University Of Michigan Biocatalyseur et procédés de synthèse de conjugués disulfure mixtes de composés de thiénopyridine
EP3858986A1 (fr) * 2020-02-03 2021-08-04 Bayer Aktiengesellschaft Variantes p450 bm3 de monooxygénase pour l'hydroxylation c19 des stéroïdes

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004099398A1 (fr) * 2003-05-09 2004-11-18 Basf Aktiengesellschaft Procede de production d'un catalyseur d'hydroxylation et son utilisation
WO2009047498A2 (fr) * 2007-10-08 2009-04-16 Isis Innovation Limited Enzymes mutantes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004099398A1 (fr) * 2003-05-09 2004-11-18 Basf Aktiengesellschaft Procede de production d'un catalyseur d'hydroxylation et son utilisation
WO2009047498A2 (fr) * 2007-10-08 2009-04-16 Isis Innovation Limited Enzymes mutantes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
APPEL D ET AL: "A P450 BM-3 mutant hydroxylates alkanes, cycloalkanes, arenes and heteroarenes", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 88, no. 2, 15 June 2001 (2001-06-15), pages 167 - 171, XP027295895, ISSN: 0168-1656, [retrieved on 20010615] *
VLADA B URLACHER ET AL: "Biotransformation of [beta]-ionone by engineered cytochrome P450 BM-3", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 70, no. 1, 1 March 2006 (2006-03-01), pages 53 - 59, XP019332079, ISSN: 1432-0614, DOI: 10.1007/S00253-005-0028-4 *

Also Published As

Publication number Publication date
WO2013076258A4 (fr) 2013-10-24
WO2013076258A2 (fr) 2013-05-30

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