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WO2013062365A3 - 변이 ｃｔｌａ4 유전자 이입 ｔ 세포 및 이를 포함하는 항암 면역치료용 조성물 - Google Patents

변이 ｃｔｌａ4 유전자 이입 ｔ 세포 및 이를 포함하는 항암 면역치료용 조성물 Download PDF

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Publication number
WO2013062365A3
WO2013062365A3 PCT/KR2012/008878 KR2012008878W WO2013062365A3 WO 2013062365 A3 WO2013062365 A3 WO 2013062365A3 KR 2012008878 W KR2012008878 W KR 2012008878W WO 2013062365 A3 WO2013062365 A3 WO 2013062365A3
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cell
ctla4
activation
chimera protein
composition including
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PCT/KR2012/008878
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French (fr)
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WO2013062365A2 (ko
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최경호
신재훈
이상진
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National Cancer Center Japan
National Cancer Center Korea
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National Cancer Center Japan
National Cancer Center Korea
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Priority to US14/354,562 priority Critical patent/US9688740B2/en
Priority to JP2014538714A priority patent/JP6074435B2/ja
Priority claimed from KR1020120119603A external-priority patent/KR101471647B1/ko
Publication of WO2013062365A2 publication Critical patent/WO2013062365A2/ko
Publication of WO2013062365A3 publication Critical patent/WO2013062365A3/ko
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    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K40/00—Cellular immunotherapy
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    • A—HUMAN NECESSITIES
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    • A61K40/00—Cellular immunotherapy
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    • A61K40/41—Vertebrate antigens
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
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    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4271—Melanoma antigens
    • A61K40/4273—Glycoprotein 100 [Gp100]
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70521—CD28, CD152
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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    • C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
    • A61K2239/38—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterised by the dose, timing or administration schedule
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
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    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

본 발명은 항암 T-세포 요법(T-cell therapy)을 위한 형질전환된 T-세포 및 이를 포함하는 항암 치료용 조성물에 대한 것으로, 구체적으로는 상기 형질전환된 T-세포는 T-세포 표면 면역관용(immune tolerance) 유도 수용체인 CTLA4 또는 PD1의 세포내 신호전달 도메인(intracellular signaling domain)을 제거하고, 이에 T-세포 활성화 표면 단백질인 CD28의 세포내 활성화 전달 도메인을 결합시킨 CTLA4-CD28 키메라(chimera) 단백질 또는 PD1-CD28 키메라 단백질을 코딩하는 유전자가 이입된 것을 특징으로 한다. 상기 CTLA4-CD28 키메라 단백질 또는 PD1-CD28 키메라 단백질을 코딩하는 유전자가 이입된 T-세포는 암 세포의 면역관용 유도로 인한 치료효과 저해 문제를 해결할 수 있을 뿐 아니라, 오히려 CD28의 신호 전달을 활성화시켜 T-세포의 활성화를 유도함으로써 함암 효과를 극대화시킬 수 있다는 장점이 있다. 또한 암세포에 특이적인 T-세포에서만 CTLA4 또는 PD1의 활성을 억제함으로써, 기존 항-CTLA4 항체 등 비특이적 CTLA4 또는 PD1 길항제(antagonist)를 이용할 경우 발생하는 전신적 T-세포 활성화로 인한 자가면역질환 발병 등의 부작용을 최소화할 수 있다.
PCT/KR2012/008878 2011-10-26 2012-10-26 변이 ｃｔｌａ4 유전자 이입 ｔ 세포 및 이를 포함하는 항암 면역치료용 조성물 Ceased WO2013062365A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US14/354,562 US9688740B2 (en) 2011-10-26 2012-10-26 Mutant CTLA4 gene transfected T cell and composition including same for anticancer immunotherapy
JP2014538714A JP6074435B2 (ja) 2011-10-26 2012-10-26 変異ｃｔｌａ４遺伝子移入ｔ細胞及びこれを含む抗がん免疫治療用組成物

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2011-0109729 2011-10-26
KR20110109729 2011-10-26
KR1020120119603A KR101471647B1 (ko) 2011-10-26 2012-10-26 변이 ｃｔｌａ4 유전자 이입 ｔ 세포 및 이를 포함하는 항암 면역치료용 조성물
KR10-2012-0119603 2012-10-26

Publications (2)

Publication Number Publication Date
WO2013062365A2 WO2013062365A2 (ko) 2013-05-02
WO2013062365A3 true WO2013062365A3 (ko) 2013-06-20

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PCT/KR2012/008878 Ceased WO2013062365A2 (ko) 2011-10-26 2012-10-26 변이 ｃｔｌａ4 유전자 이입 ｔ 세포 및 이를 포함하는 항암 면역치료용 조성물

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11851678B2 (en) 2017-08-09 2023-12-26 Juno Therapeutics, Inc. Methods for producing genetically engineered cell compositions and related compositions

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2865033C (en) 2012-02-23 2021-11-02 Stage Cell Therapeutics Gmbh Chromatographic isolation of cells and other complex biological materials
WO2015164675A1 (en) 2014-04-23 2015-10-29 Juno Therapeutics, Inc. Methods for isolating, culturing, and genetically engineering immune cell populations for adoptive therapy
FI3909972T3 (fi) * 2015-06-19 2024-05-03 Sebastian Kobold Pd1-cd28-fuusioproteiineja ja niiden käyttö lääkkeessä
RU2021134624A (ru) 2015-10-22 2022-03-15 Джуно Терапьютикс Гмбх Способы, наборы, средства и устройства для трансдукции
BR112020008340A2 (pt) 2017-11-01 2020-11-17 Juno Therapeutics Inc processo para gerar composições terapêuticas de células modificadas
KR20240088571A (ko) * 2022-11-18 2024-06-20 서울대학교산학협력단 Ctla-4 변이체 및 키메라 항원수용체를 이용한 이중 유전자 이입 면역세포 및 이의 용도

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6090914A (en) * 1991-06-27 2000-07-18 Bristol-Myers Squibb Company CTLA4/CD28Ig hybrid fusion proteins and uses thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6090914A (en) * 1991-06-27 2000-07-18 Bristol-Myers Squibb Company CTLA4/CD28Ig hybrid fusion proteins and uses thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CARTELLIERI, M. ET AL.: "Chimeric antigen receptor-engineered T cells for immunotherapy of cancer", JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, vol. 2010, 2010, pages 13 *
JENA, B. ET AL.: "Redirecting T-cell specificity by introducing a tumor-specific chimeric antigen receptor", BLOOD, vol. 116, no. 7, 3 May 2010 (2010-05-03), pages 1035 - 1044, XP055021403, DOI: doi:10.1182/blood-2010-01-043737 *
SHIN, J.H. ET AL.: "Positive conversion of negative signaling of CTLA4 potentiates antitumor efficacy of adoptive T-cell therapy in murine tumor models", BLOOD, vol. 119, no. 24, 26 April 2012 (2012-04-26), pages 5678 - 87, XP002743489, DOI: doi:10.1182/blood-2011-09-380519 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11851678B2 (en) 2017-08-09 2023-12-26 Juno Therapeutics, Inc. Methods for producing genetically engineered cell compositions and related compositions

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