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WO2013058868A3 - Dosage multiplex pour la détection de mutations kras - Google Patents

Dosage multiplex pour la détection de mutations kras Download PDF

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Publication number
WO2013058868A3
WO2013058868A3 PCT/US2012/052377 US2012052377W WO2013058868A3 WO 2013058868 A3 WO2013058868 A3 WO 2013058868A3 US 2012052377 W US2012052377 W US 2012052377W WO 2013058868 A3 WO2013058868 A3 WO 2013058868A3
Authority
WO
WIPO (PCT)
Prior art keywords
multiplexed
detection assay
mutation detection
kras mutation
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/052377
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English (en)
Other versions
WO2013058868A2 (fr
Inventor
Rebecca Oldham-Haltom
Hatim Allawi
Hongzhi Zou
Michael J. Domanico
Graham P. Lidgard
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Exact Sciences Corp
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Exact Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exact Sciences Corp filed Critical Exact Sciences Corp
Priority to EP12841997.5A priority Critical patent/EP2748339B1/fr
Priority to CA2849020A priority patent/CA2849020C/fr
Publication of WO2013058868A2 publication Critical patent/WO2013058868A2/fr
Priority to AU2014100267A priority patent/AU2014100267A4/en
Anticipated expiration legal-status Critical
Publication of WO2013058868A3 publication Critical patent/WO2013058868A3/fr
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention concerne un mélange de réactifs comprenant des réactifs d'amplification et des réactifs de dosage de flap multiplex pour détecter, au cours d'une seule et même réaction, des copies mutantes du gène KRAS qui contiennent l'une quelconque des mutations ponctuelles 34A, 34C, 34T, 35A, 35C, 35T ou 38A. Des procédés utilisant le mélange de réactifs selon l'invention et des kits pour les mettre en œuvre sont également décrits.
PCT/US2012/052377 2011-10-18 2012-08-24 Dosage multiplex pour la détection de mutations kras Ceased WO2013058868A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP12841997.5A EP2748339B1 (fr) 2011-10-18 2012-08-24 Dosage multiplex pour la détection de mutations kras
CA2849020A CA2849020C (fr) 2011-10-18 2012-08-24 Dosage multiplex pour la detection de mutations kras
AU2014100267A AU2014100267A4 (en) 2011-10-18 2014-03-20 Multiplexed KRAS mutation detection assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161548639P 2011-10-18 2011-10-18
US61/548,639 2011-10-18

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2014100267A Division AU2014100267A4 (en) 2011-10-18 2014-03-20 Multiplexed KRAS mutation detection assay

Publications (2)

Publication Number Publication Date
WO2013058868A2 WO2013058868A2 (fr) 2013-04-25
WO2013058868A3 true WO2013058868A3 (fr) 2014-05-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/052377 Ceased WO2013058868A2 (fr) 2011-10-18 2012-08-24 Dosage multiplex pour la détection de mutations kras

Country Status (4)

Country Link
US (7) US9127318B2 (fr)
EP (1) EP2748339B1 (fr)
CA (1) CA2849020C (fr)
WO (1) WO2013058868A2 (fr)

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US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
US9212392B2 (en) 2012-09-25 2015-12-15 Exact Sciences Corporation Normalization of polymerase activity
WO2015066695A1 (fr) * 2013-11-04 2015-05-07 Exact Sciences Corporation Calibreurs de commande multiple pour l'analyse quantitative d'adn
EP3084004A4 (fr) * 2013-12-19 2017-08-16 Exact Sciences Corporation Molécules témoins d'acides nucléiques de synthèse
US10184154B2 (en) 2014-09-26 2019-01-22 Mayo Foundation For Medical Education And Research Detecting cholangiocarcinoma
AU2015360352B2 (en) 2014-12-12 2022-01-27 Exact Sciences Corporation Compositions and methods for performing methylation detection assays
US10011878B2 (en) 2014-12-12 2018-07-03 Exact Sciences Development Company Compositions and methods for performing methylation detection assays
CN107532124B (zh) 2015-03-27 2022-08-09 精密科学公司 检测食管疾病
CN104862401B (zh) * 2015-05-29 2017-10-31 沈阳优吉诺生物科技有限公司 检测kras基因热点突变位点的引物、试剂盒及其pcr方法
EP3998353A1 (fr) * 2015-10-30 2022-05-18 Exact Sciences Corporation Détection par amplification multiplexée et isolement et détection d'adn issu de plasma
EP3488004B1 (fr) 2016-07-19 2021-10-06 Exact Sciences Development Company, LLC Adn témoin méthylé
KR102667067B1 (ko) 2017-01-27 2024-05-17 이그젝트 싸이언스 디블롭먼트 컴패니, 엘엘씨 메틸화된 dna 분석에 의한 결장 신조직형성의 검출
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
CN108998535B (zh) * 2018-09-11 2021-09-10 广州市宝创生物技术有限公司 一种kras基因多重突变位点检测试剂盒
CA3127620A1 (fr) * 2019-01-25 2020-07-30 Gen-Probe Incorporated Detection de mycoplasma genitalium resistant aux medicaments
CN119614682A (zh) * 2021-09-30 2025-03-14 简·探针公司 可温度选择的fret盒信号传导

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US20200270706A1 (en) 2020-08-27
US20220333210A1 (en) 2022-10-20
US20130231256A1 (en) 2013-09-05
CA2849020A1 (fr) 2013-04-25
US11352674B2 (en) 2022-06-07
WO2013058868A2 (fr) 2013-04-25
US12060620B2 (en) 2024-08-13
US20180066319A1 (en) 2018-03-08
US9127318B2 (en) 2015-09-08
US20160010161A1 (en) 2016-01-14
US9783856B2 (en) 2017-10-10
US20190055609A1 (en) 2019-02-21
EP2748339A2 (fr) 2014-07-02
US10093987B2 (en) 2018-10-09
EP2748339B1 (fr) 2017-09-27
CA2849020C (fr) 2023-05-09
US20240417810A1 (en) 2024-12-19
US10626465B2 (en) 2020-04-21
EP2748339A4 (fr) 2015-06-17

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