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WO2013055142A3 - Procédé de fabrication d'un conjugué multifonctionnel de biomatériau au moyen de deux types de particule et conjugué multifonctionnel de biomatériau obtenu grâce à ce procédé - Google Patents

Procédé de fabrication d'un conjugué multifonctionnel de biomatériau au moyen de deux types de particule et conjugué multifonctionnel de biomatériau obtenu grâce à ce procédé Download PDF

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Publication number
WO2013055142A3
WO2013055142A3 PCT/KR2012/008301 KR2012008301W WO2013055142A3 WO 2013055142 A3 WO2013055142 A3 WO 2013055142A3 KR 2012008301 W KR2012008301 W KR 2012008301W WO 2013055142 A3 WO2013055142 A3 WO 2013055142A3
Authority
WO
WIPO (PCT)
Prior art keywords
bio
conjugate
functional bio
material conjugate
manufacturing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2012/008301
Other languages
English (en)
Korean (ko)
Other versions
WO2013055142A2 (fr
Inventor
김민곤
성윤주
변주영
오영경
정효암
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gwangju Institute of Science and Technology
Korea Research Institute of Bioscience and Biotechnology KRIBB
Original Assignee
Gwangju Institute of Science and Technology
Korea Research Institute of Bioscience and Biotechnology KRIBB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gwangju Institute of Science and Technology, Korea Research Institute of Bioscience and Biotechnology KRIBB filed Critical Gwangju Institute of Science and Technology
Priority to US14/351,291 priority Critical patent/US9880162B2/en
Priority to CN201280050559.4A priority patent/CN104011545B/zh
Publication of WO2013055142A2 publication Critical patent/WO2013055142A2/fr
Publication of WO2013055142A3 publication Critical patent/WO2013055142A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/02—Linear peptides containing at least one abnormal peptide link
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y10S977/773—Nanoparticle, i.e. structure having three dimensions of 100 nm or less
    • Y10S977/774—Exhibiting three-dimensional carrier confinement, e.g. quantum dots

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Nanotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Manufacturing & Machinery (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Organic Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé de fabrication d'un conjugué multifonctionnel de biomatériau et un conjugué multifonctionnel de biomatériau obtenu grâce à ce procédé. Plus précisément, l'invention concerne un conjugué multifonctionnel de biomatériau utilisé comme biocapteur pour détecter des micro-organismes ou des organismes similaires. Le procédé de l'invention comprend : (a) une étape de revêtement d'une protéine sur une première nanoparticule possédant des caractéristiques magnétiques ou fluorescentes ; (b) une étape de fabrication d'un conjugué par l'adhérence d'une seconde nanoparticule possédant des caractéristiques métalliques à la première nanoparticule recouverte de protéines ; et (c) une étape de fabrication d'un conjugué multifonctionnel de biomatériau par l'adhérence d'un biomatériau au conjugué. Grâce à l'utilisation de deux types de particule, la précipitation des nanoparticules est évitée, le biomatériau se fixe facilement et un conjugué de biomatériau à multiples fonctions peut être fabriqué. En outre, le conjugué multifonctionnel de biomatériau fabriqué grâce au procédé de l'invention peut être utilisé pour détecter des micro-organismes à une concentration allant jusqu'à 101 cfu.
PCT/KR2012/008301 2011-10-14 2012-10-12 Procédé de fabrication d'un conjugué multifonctionnel de biomatériau au moyen de deux types de particule et conjugué multifonctionnel de biomatériau obtenu grâce à ce procédé Ceased WO2013055142A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US14/351,291 US9880162B2 (en) 2011-10-14 2012-10-12 Method for manufacturing multi-functional bio-material conjugate using two kinds of particle, and multi-functional bio-material conjugate manufactured by means of same
CN201280050559.4A CN104011545B (zh) 2011-10-14 2012-10-12 利用两种粒子的多功能生物材料接合体的制备方法及从中制得的多功能生物材料接合体

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0105463 2011-10-14
KR20110105463 2011-10-14

Publications (2)

Publication Number Publication Date
WO2013055142A2 WO2013055142A2 (fr) 2013-04-18
WO2013055142A3 true WO2013055142A3 (fr) 2013-07-04

Family

ID=48082670

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/008301 Ceased WO2013055142A2 (fr) 2011-10-14 2012-10-12 Procédé de fabrication d'un conjugué multifonctionnel de biomatériau au moyen de deux types de particule et conjugué multifonctionnel de biomatériau obtenu grâce à ce procédé

Country Status (4)

Country Link
US (1) US9880162B2 (fr)
KR (1) KR101444424B1 (fr)
CN (1) CN104011545B (fr)
WO (1) WO2013055142A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101427146B1 (ko) * 2013-11-13 2014-08-07 단국대학교 산학협력단 생체 분자 분석 키트 및 이를 이용한 생체 분자 분석 방법
TWI586807B (zh) * 2013-11-21 2017-06-11 中央研究院 偵測檢體內金黃色葡萄球菌的方法
CN104237501B (zh) * 2014-08-13 2016-04-20 南京大学 一种抗体修饰的纳米银及其试剂盒与应用
CN106680345A (zh) * 2016-12-20 2017-05-17 深圳大学 磁性复合纳米材料、电化学生物传感器及制备、构建方法
CN106770135A (zh) * 2017-01-25 2017-05-31 东南大学 金属增强荧光信号的编码微球及其制备方法和应用
US20180284110A1 (en) * 2017-03-30 2018-10-04 Imra America, Inc. Clustered precious metal nanoparticles in a stable colloidal suspension and biological applications using the same
CN108318694A (zh) * 2018-01-29 2018-07-24 厦门大学 一种采用免疫试纸条对抗原进行定量检测的方法
KR102426791B1 (ko) * 2018-08-09 2022-07-28 (주) 비비비 자성 나노입자를 이용한 바이오센서, 이를 이용하는 검출 장치 및 검출 방법
JP7104798B2 (ja) * 2018-09-28 2022-07-21 富士フイルム株式会社 免疫クロマトグラフィー用複合粒子及びその製造方法、並びに、免疫クロマトグラフィー
EP3951394A4 (fr) * 2019-03-29 2022-04-20 FUJIFILM Corporation Immunochromatographie
KR102439357B1 (ko) * 2021-02-17 2022-09-02 서울대학교산학협력단 변환기능 링커 기반의 금 나노입자의 응집 시스템을 이용한 현장용 유해물질 검출키트 및 그를 이용한 유해물질의 검출방법
FR3134822A1 (fr) * 2022-04-26 2023-10-27 bioMérieux milieu réactionnel et méthode associée pour la détection, d’un micro-organisme cible.
EP4484569A1 (fr) * 2023-06-29 2025-01-01 Biomérieux Milieu réactionnel et méthode permettant la détection d'une e. coli productrice de shigatoxine et/ou d'une e. coli entérohémorragique

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010058711A (ko) * 1999-12-30 2001-07-06 박종섭 적층 위상반전 패턴을 구비하는 위상반전 마스크
US20030082237A1 (en) * 2001-10-02 2003-05-01 Jennifer Cha Nanoparticle assembled hollow spheres
US20090117002A1 (en) * 2005-05-27 2009-05-07 Ohio University Nanoparticle assemblies with molecular springs
KR20090116142A (ko) * 2008-05-06 2009-11-11 한국식품연구원 식중독균 검출을 위한 자성 코어 금 나노입자 및 그제조방법, 상기 나노입자를 이용한 식중독균 검출방법

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60017017T2 (de) * 1999-01-27 2006-04-13 Bayer Corp. Immuntest unter verwendung von partikeln mit kaseinumhüllungen
ATE423971T1 (de) * 2000-12-11 2009-03-15 Mitsubishi Kagaku Iatron Inc Immunologisches verfahren
EP1903337B1 (fr) * 2006-09-20 2015-07-22 Mycartis N.V. Revêtement pour microporteurs
US7868145B2 (en) * 2007-07-11 2011-01-11 Industrial Technology Research Institute Magnetic particles containing a copolymer core, magnetic layer and silicon layer
WO2011065747A2 (fr) * 2009-11-24 2011-06-03 한국생명공학연구원 Procédé de détection rapide de micro-organismes au moyen de nanoparticules

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010058711A (ko) * 1999-12-30 2001-07-06 박종섭 적층 위상반전 패턴을 구비하는 위상반전 마스크
US20030082237A1 (en) * 2001-10-02 2003-05-01 Jennifer Cha Nanoparticle assembled hollow spheres
US20090117002A1 (en) * 2005-05-27 2009-05-07 Ohio University Nanoparticle assemblies with molecular springs
KR20090116142A (ko) * 2008-05-06 2009-11-11 한국식품연구원 식중독균 검출을 위한 자성 코어 금 나노입자 및 그제조방법, 상기 나노입자를 이용한 식중독균 검출방법

Also Published As

Publication number Publication date
KR20130040715A (ko) 2013-04-24
CN104011545A (zh) 2014-08-27
KR101444424B1 (ko) 2014-09-29
WO2013055142A2 (fr) 2013-04-18
CN104011545B (zh) 2016-02-17
US20140272945A1 (en) 2014-09-18
US9880162B2 (en) 2018-01-30

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