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WO2012135591A3 - Microbial isoprenoid production using a heterologous dxp pathway - Google Patents

Microbial isoprenoid production using a heterologous dxp pathway Download PDF

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Publication number
WO2012135591A3
WO2012135591A3 PCT/US2012/031395 US2012031395W WO2012135591A3 WO 2012135591 A3 WO2012135591 A3 WO 2012135591A3 US 2012031395 W US2012031395 W US 2012031395W WO 2012135591 A3 WO2012135591 A3 WO 2012135591A3
Authority
WO
WIPO (PCT)
Prior art keywords
heterologous
dxp pathway
isoprenoid production
microbial
microbial isoprenoid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/031395
Other languages
French (fr)
Other versions
WO2012135591A2 (en
Inventor
Lishan Zhao
Kevin L. DIETZEL
Gale A. WICHMANN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amyris Inc
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of WO2012135591A2 publication Critical patent/WO2012135591A2/en
Anticipated expiration legal-status Critical
Publication of WO2012135591A3 publication Critical patent/WO2012135591A3/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides methods for a robust production of isoprenoids utilizing the DXP biosynthetic pathway. The invention also provides nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods.
PCT/US2012/031395 2011-03-30 2012-03-30 Microbial isoprenoid production using a heterologous dxp pathway Ceased WO2012135591A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161469767P 2011-03-30 2011-03-30
US61/469,767 2011-03-30

Publications (2)

Publication Number Publication Date
WO2012135591A2 WO2012135591A2 (en) 2012-10-04
WO2012135591A3 true WO2012135591A3 (en) 2014-05-01

Family

ID=46932358

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/031395 Ceased WO2012135591A2 (en) 2011-03-30 2012-03-30 Microbial isoprenoid production using a heterologous dxp pathway

Country Status (1)

Country Link
WO (1) WO2012135591A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9920312B2 (en) * 2014-08-11 2018-03-20 Lallemand Hungary Liquidity Management Llc Mutations in iron-sulfur cluster proteins that improve xylose utilization
CN109804073B (en) 2016-08-12 2023-05-16 阿迈瑞斯公司 UDP-dependent glycosyltransferase for efficient rebaudioside production
CN108624516B (en) * 2017-03-20 2022-08-26 华东理工大学 Method for improving metabolite amount in fermentation cells and preparing IDMS standard substance
CN109234216B (en) * 2017-07-10 2022-05-20 上海医药工业研究院 A kind of genetic engineering bacteria for producing squalene and method thereof
MA49836A (en) 2017-08-11 2020-06-17 Amyris Inc PISUM SATIVUM KAURENE OXIDASE FOR THE HIGH YIELD PRODUCTION OF REBAUDIOSIDES
JP2021505154A (en) 2017-12-07 2021-02-18 ザイマージェン インコーポレイテッド Designed biosynthetic pathway for producing (6E) -8-hydroxygeraniol by fermentation
WO2019126778A1 (en) 2017-12-21 2019-06-27 Zymergen Inc. Nepetalactol oxidoreductases, nepetalactol synthases, and microbes capable of producing nepetalactone
CN110468089B (en) * 2018-05-09 2023-07-11 深圳艾格鑫科技有限公司 Microorganism and use thereof
CN108715825B (en) * 2018-06-01 2021-03-09 天津大学 Gene overexpression, strain obtained by gene overexpression and application of gene overexpression
AU2019360932A1 (en) 2018-10-15 2021-05-06 Amyris, Inc. Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides
US20220106619A1 (en) 2019-01-24 2022-04-07 Amyris, Inc. Abc transporters for the high efficiency production of rebaudiosides
CN115806921B (en) * 2021-09-14 2025-06-27 武汉合生科技有限公司 A recombinant microorganism for producing farnesene and lycopene
WO2025111429A1 (en) 2023-11-22 2025-05-30 Bp Corporation North America Inc. Recombinant microorganisms with increased accumulation and/or flux of cytidine triphosphate (ctp)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090275104A1 (en) * 2004-01-09 2009-11-05 Novozymes A/S Bacillus licheniformis chromosone
US20110014672A1 (en) * 2009-06-17 2011-01-20 Chotani Gopal K Isoprene production using the dxp and mva pathway

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090275104A1 (en) * 2004-01-09 2009-11-05 Novozymes A/S Bacillus licheniformis chromosone
US20110014672A1 (en) * 2009-06-17 2011-01-20 Chotani Gopal K Isoprene production using the dxp and mva pathway

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FARHI ET AL.: "Harnessing yeast subcellular compartments for the production of plant terpenoids.", METABOLIC ENGINEERING, vol. 13, 18 May 2011 (2011-05-18), pages 474 - 481, XP028274814, DOI: doi:10.1016/j.ymben.2011.05.001 *
MAURY ET AL.: "Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae.", FEBS LETT, vol. 582, 2008, pages 4032 - 4038, XP025745917, DOI: doi:10.1016/j.febslet.2008.10.045 *
NETZ ET AL.: "The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol.", NATURE CHEMICAL BIOLOGY, vol. 3, 2007, pages 278 - 286 *

Also Published As

Publication number Publication date
WO2012135591A2 (en) 2012-10-04

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