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WO2012103915A1 - Contenant de filtration pour macromolécules - Google Patents

Contenant de filtration pour macromolécules Download PDF

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Publication number
WO2012103915A1
WO2012103915A1 PCT/EP2011/006577 EP2011006577W WO2012103915A1 WO 2012103915 A1 WO2012103915 A1 WO 2012103915A1 EP 2011006577 W EP2011006577 W EP 2011006577W WO 2012103915 A1 WO2012103915 A1 WO 2012103915A1
Authority
WO
WIPO (PCT)
Prior art keywords
filtration
main wall
chamber
container according
filtration container
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2011/006577
Other languages
German (de)
English (en)
Inventor
Christopher Biddell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sartorius Stedim Biotech GmbH
Original Assignee
Sartorius Stedim Biotech GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sartorius Stedim Biotech GmbH filed Critical Sartorius Stedim Biotech GmbH
Publication of WO2012103915A1 publication Critical patent/WO2012103915A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B01L2300/047—Additional chamber, reservoir
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter

Definitions

  • the invention relates to a filtration container, in particular for the concentration of macromolecules, which can be inserted into a filtrate recovery tube, with an upper portion, an axially to the upper portion
  • the upper section forms a sample container which is hollow-cylindrical or formed as a hollow truncated cone and has an inlet opening at its upper end side,
  • the downwardly tapering central portion has a filtration chamber having at least one planar first main wall tapering downwardly relative to the opposite wall, the at least one main planar wall having a filter window sealed by a laminar membrane filter and the lower one Section one to the lower edge of the
  • a concentration of macromolecules in particular of
  • Protein samples in a volume range of 500 ⁇ to 20 ml in filtration containers, in a
  • Centrifugal units are concentrated by the method of ultrafiltration.
  • ultrafiltration it is common practice to provide a retentate chamber as a so-called dead-stop pocket in the filtration containers to prevent dry running of the sample.
  • the concentrated sample is collected in the dead volume formed by the retentate chamber and recovered by pipetting.
  • EP 0 651 675 B1 discloses a filtration tank for
  • the known filtration tank consists of an upper portion, one axially to the upper portion
  • the upper portion forms a sample container, which is formed as a hollow cylinder, and the downwardly tapered middle
  • Section forms a filtration chamber, which has two mutually opposite downwardly converging main walls and two connecting them at their lateral edges connecting walls.
  • One of the main walls forms a filter window for
  • Extensions of the main and connecting walls of the middle section are.
  • JP 62-011564 A1 discloses a filtration vessel for the centrifugal concentration of macromolecules.
  • a substantially hollow cylindrical container has a cylindrical insert which divides the container in the longitudinal direction and has a sealing collar.
  • an obliquely cut approach is arranged, whose inlet opening is covered by a filter membrane, which thus rises by an angle between 25 and 45 °.
  • a formed annular RetentatKammer in which the retentate is collected.
  • the volume of the dead-stop pocket which here also adjoins the boundary region to the membrane, can increase in a tolerance range by changing the membrane permeability as a result of adhesive or thermal action.
  • Object of the present invention is therefore, a
  • Specify filtration tank which has a retentate chamber, which allows the setting of a constant volume after the concentration in the retentate.
  • Retentate chamber is always the same and thus reproducible by their inventive design of filtration tank to filtration tank.
  • the filter window runs pointed towards its lower edge.
  • the active membrane with the lower edge of the window has practically only a point contact with negligible membrane area and thus does not lead to a distortion of the retentate volume.
  • Filtration chamber on the outside facing a filter cover with at least one filtrate on.
  • Invention is the filter cover as adapted to the outer shape of the filtration chamber überverschbare sleeve
  • the membrane can be in the filter window at a
  • planar main wall and the opposite wall of the filtration chamber converge toward one another. Due to the converging main walls, the emergence or the formation of a small volume retentate chamber is favored.
  • the first main wall of the filtration chamber is over one opposite to a transverse to a longitudinal axis
  • Horizontal sloping slope connected to an adjacent first main wall of the retentate chamber.
  • the sloping slope has an angle of greater than or equal to 30 ° relative to the horizontal.
  • the sloping Sloped to the horizontal an angle of about 45 °.
  • At least the retentate chamber is made of a chemically inert material, in particular of the polymer polystyrene,
  • Polystyrene has the advantage that it is chemically inert and has no protein binding properties.
  • the collection of the concentrated sample or of the retentate is made possible in a very small volume space, which is also chemically inert.
  • FIG. 1 shows a spatial representation of a
  • Figure 2 a side view in section of the
  • FIG. 3 an enlarged view of the detail III of FIG. 2. Description of the embodiments
  • a filtration tank 1 essentially consists of an upper section 2, a middle section 3 and a lower section 4.
  • the filtration tank 1 is in a
  • Filtrate recovery tube 5 can be used. It is the
  • the upper portion 2 forms a sample container 8, which is formed substantially hollow-cylindrical or as a hollow truncated cone and at its upper end face 9 a
  • Inlet opening 10 has.
  • the central portion 3 tapers in a vertical plane downwards and forms a filtration chamber 13, the two opposing downwards
  • the first main wall 14 is flat
  • a filter window 18 is arranged, which is on its the filtration chamber 13th
  • the filter window 18 has a transverse to the longitudinal axis 20 of the filtration tank 1 extending lower edge 21, to which the lower portion 4 connects in the vertical direction downwards.
  • the filter window 18 arranged in the plane first main wall 14 is at its retentate chamber 22
  • the filter window 18 laterally bounding edges 32, 33 taper toward each other.
  • the lower section 4 forms a retentate chamber 22 with a first planar main wall 23 and an opposite second, semicircular or concave main wall 24.
  • the first main wall 14 of the filtration chamber 13 is disposed opposite one transverse to the longitudinal axis 20
  • Retentate chamber 22 connected.
  • the retentate chamber 22 is vertically downwardly from a bottom 30th
  • Retentate volume 34 (dead space).
  • the filter cover 31 has at least one filtrate channel 35 through which the through the membrane filter 19th
  • the filter cover 31 is formed as an over-sleeve adapted to the outer shape of the middle portion 4 formed by the filtration chamber 13.
  • the filtration tank 1 is preferably formed from the polymer polystyrene.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un contenant de filtration (1) pour la concentration de macromolécules, pouvant être utilisé dans un tube (5) d'extraction de filtrat et comprenant une section supérieure (2), une section centrale (3) dans le prolongement axial de la section supérieure (2) et une section inférieure (4) dans le prolongement axial de la section centrale (3). La section supérieure (2) forme un contenant d'échantillons (8) doté d'un orifice d'entrée (10) et la section centrale (3) effilée vers le bas présente une chambre de filtration (13) dotée de deux parois principales (14, 15), la paroi principale (14) présentant une fenêtre de filtre (18) qui est hermétiquement fermée par une membrane filtrante (19) plate. La section inférieure (4) forme une chambre de rétentat (22) dans le prolongement du bord inférieur (21) de la fenêtre filtre (18) vers le bas dans la direction verticale, la fenêtre de filtre (18) disposée dans la première paroi principale (14) plate est effilée jusqu'à son bord inférieur (21) qui est adjacent à la chambre de rétentat (22).
PCT/EP2011/006577 2011-01-31 2011-12-27 Contenant de filtration pour macromolécules Ceased WO2012103915A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE202011002150.5 2011-01-31
DE202011002150U DE202011002150U1 (de) 2011-01-31 2011-01-31 Filtrationsbehälter

Publications (1)

Publication Number Publication Date
WO2012103915A1 true WO2012103915A1 (fr) 2012-08-09

Family

ID=44503121

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/006577 Ceased WO2012103915A1 (fr) 2011-01-31 2011-12-27 Contenant de filtration pour macromolécules

Country Status (2)

Country Link
DE (1) DE202011002150U1 (fr)
WO (1) WO2012103915A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111632635A (zh) * 2020-07-06 2020-09-08 广州洁特生物过滤股份有限公司 一种超滤离心管装置

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6211564A (ja) 1985-07-09 1987-01-20 Toyo Soda Mfg Co Ltd 遠心型口過用容器
US5310527A (en) * 1992-12-14 1994-05-10 E. I. Du Pont De Nemours And Company Tube for use in a pelleting centrifuge rotor
US5647990A (en) * 1993-05-21 1997-07-15 Vassarotti; Vincenzo Centrifugal method for concentrating macromolecules from a solution and device for carrying out said method
DE20002188U1 (de) * 1999-02-15 2000-05-11 Sartorius AG, 37075 Göttingen Vorrichtung zum Konzentrieren und/oder Reinigen von Makromolekülen in einer Lösung
US6156199A (en) * 1997-08-11 2000-12-05 Zuk, Jr.; Peter Centrifugal filtration apparatus
US20090078638A1 (en) * 2007-09-24 2009-03-26 Louis Bonhomme Centrifugal filter

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6211564A (ja) 1985-07-09 1987-01-20 Toyo Soda Mfg Co Ltd 遠心型口過用容器
US5310527A (en) * 1992-12-14 1994-05-10 E. I. Du Pont De Nemours And Company Tube for use in a pelleting centrifuge rotor
US5647990A (en) * 1993-05-21 1997-07-15 Vassarotti; Vincenzo Centrifugal method for concentrating macromolecules from a solution and device for carrying out said method
EP0651675B1 (fr) 1993-05-21 1997-10-29 Vincenzo Vassarotti Procede de concentration de macromolecules en solution par centrifugation, et appareil associe
US6156199A (en) * 1997-08-11 2000-12-05 Zuk, Jr.; Peter Centrifugal filtration apparatus
DE20002188U1 (de) * 1999-02-15 2000-05-11 Sartorius AG, 37075 Göttingen Vorrichtung zum Konzentrieren und/oder Reinigen von Makromolekülen in einer Lösung
US20090078638A1 (en) * 2007-09-24 2009-03-26 Louis Bonhomme Centrifugal filter

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111632635A (zh) * 2020-07-06 2020-09-08 广州洁特生物过滤股份有限公司 一种超滤离心管装置

Also Published As

Publication number Publication date
DE202011002150U1 (de) 2011-07-20

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