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WO2012167341A1 - Procédé d'utilisation de membrane de cellulose bactérienne intra-articulaire sur surface lésée de rotule - Google Patents

Procédé d'utilisation de membrane de cellulose bactérienne intra-articulaire sur surface lésée de rotule Download PDF

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Publication number
WO2012167341A1
WO2012167341A1 PCT/BR2012/000179 BR2012000179W WO2012167341A1 WO 2012167341 A1 WO2012167341 A1 WO 2012167341A1 BR 2012000179 W BR2012000179 W BR 2012000179W WO 2012167341 A1 WO2012167341 A1 WO 2012167341A1
Authority
WO
WIPO (PCT)
Prior art keywords
membrane
articular
bacterial cellulose
tissue
cellulose membrane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/BR2012/000179
Other languages
English (en)
Portuguese (pt)
Inventor
Ana Maria Minarelli Gaspar
Cassiano de Moura ABDALLA
Sidney José Lima RIBEIRO
Sybele Saska
Younès MESSADDEQ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidade de Sao Paulo USP
Universidade Estadual Paulista Julio de Mesquita Filho UNESP
Original Assignee
Universidade de Sao Paulo USP
Universidade Estadual Paulista Julio de Mesquita Filho UNESP
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidade de Sao Paulo USP, Universidade Estadual Paulista Julio de Mesquita Filho UNESP filed Critical Universidade de Sao Paulo USP
Publication of WO2012167341A1 publication Critical patent/WO2012167341A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3637—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the origin of the biological material other than human or animal, e.g. plant extracts, algae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • A61L27/3645—Connective tissue
    • A61L27/3662—Ligaments, tendons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/10—Materials or treatment for tissue regeneration for reconstruction of tendons or ligaments

Definitions

  • the present invention discloses the use of bacterial cellulose membrane for application in ligamentous, tendon and synovial capsule reconstructions according to the methods described in the description of the technique of the invention. This material would be applicable for ligament and tendon reconstruction (knee cruciate ligaments, patellar ligament, Achilles tendon, quadriceps tendon, etc.) and synovial capsule.
  • ACL injury is the most common intra-articular knee injury (Freeman et al, 2007). Annually, more than 200,000 patients are diagnosed with ACL rupture in the US (Beynnon & Fleming, 1998; Pennisi, 2002) and approximately 150,000 are surgically treated (Cameron et al., 2000; Cooper et al, 2002).
  • grafts from localities near or far from the injured ones (autogenous grafts), where the patellar, quadriceps, semitendinous, gracile tendon and the Achilles tendon are commonly chosen. however, morbidity due to the removal of these grafts or pain at the place where the graft was removed become common complications of using this material for ACL reconstruction (Cartmell & Dunn, 2004).
  • allografts or allograft transplants of the mentioned structures are also applied in some hospitals, restricted to limited supply, besides possible sites of bacterial infection or even transmission of other infectious diseases, or a possible immune response in the host organism (Cameron et al., 2000; Cartmell & Dunn, 2004; Vunjak-Novakovic et al., 2004).
  • the third and final treatment option for possible injuries are synthetic prostheses when related to ligamentous or tendon injuries where non-degradable synthetic materials such as carbon fiber, polyethylene, polypropylene and polytetrafluoroethylene are used.
  • the present invention allows for a wide evolution in the field of repair of capsular, tendon and ligament injuries.
  • the described technique allows biological regeneration of the injured tissues with fast, efficient, immediate and affordable treatments to repair these injuries.
  • the result is obtained without damage to the musculoskeletal tissue, occurring as an innovation of past techniques, that is, a new tissue is added to the body without the association of mutilation of other structures.
  • This treatment technique may or may not have the association of hyaluronic acid or collagen derivatives to the proposed model.
  • the ideal matrix for the synthetic model to be manufactured should be biodegradable, porous and have sufficient mechanical strength for the deformation required during the force applied to it (Cooper et al., 2007). All of these products were invented with these properties in mind, but in all of them, there are always some of these properties missing to become the "gold model" to be indicated in surgery as the first choice.
  • the present invention is odd in relation to the production technique and origin of the materials that are necessary for its manufacture, since its whole process is of biological nature and totally biocompatible, has all the properties mentioned above, that is, biodegradable and biocompatible.
  • the bacterial cellulose membrane is porous and if used as described, becomes It has a very high mechanical resistance, adapting perfectly to the deformations required and for no moment loses its mechanical resistance. It is very important to mention that the membrane developed with the technique passed an ethibond # 5 thread fixation tensile test, and that the rupture at 182 N occurred in the tissue laceration with the thread.
  • histological section slides shown in this document confirm that the membrane over time engages the bone in a blended form, making it nearly impossible to separate them. And obviously, when these fibers stick to the bone tunnels where they will be inserted, their mechanical strengths will be much higher.
  • Figure 1 demonstrates the tensile strength of the material.
  • Figure 2 shows the material positioned next to the caliper before the tensile test.
  • Figure 3 shows the test of the material under traction.
  • Figure 4 shows the material in the tensile tester supporting the tensile.
  • Figure 5 shows the material during the tensile test undergoing rupture.
  • Figure 6 shows the graph of the product deformation curve.
  • Acetobacter xyllinus membrane was isolated as a biofilm after 72 to 96 hours of culture. Isolated with sodium hypochlorite solution for 30 minutes, followed by thorough washing with distilled water and then sterilized in humid heat at 122 ° C.
  • this prepared material was transfixed with catgut-0 wires on the patella articular surface of the three groups of animals.
  • a highly resistant connective tissue was observed and after six months, this tissue strongly adhered to the bone.
  • the idea arose to verify in another animal, the material prepared without transfixation in the patella, inserted only in the bony ends of the tibia and femur, because we verified that the cellulose membrane offered conditions for the organism itself. produce a transformation in that tissue so that it provides similar or even greater resistance to connective tissues in general. What makes this result very interesting is the application of the membrane when used inside a synovial joint, because it was nontoxic and without any antigenic properties, much less if used in extra-articular region.
  • the tissue formed did not generate any sign of inflammation or predisposition to an infectious condition, unlike the contralateral knee, which had the same size lesion, but without the application of the membrane model.
  • this group considered the control group (CG)
  • two cases evolved with dehiscence of purulent sutures and secretions different from the membrane treated group (GT), which presented healing without secretion or inflammatory signs in all 12 operated animals, and which is the most important, without animals limping (limping) with the limb on the treated side during gait.
  • ligament reconstructions such as the anterior cruciate ligament of the knee and other forms of application of the material
  • Current techniques for ligament repair include removal of tendon-removed (quadriceps, semitendine, and gracilis, and patellar) grafts with possible painful clinical symptoms associated or not with inflammatory processes at the donor site or loss of muscle action when the removal is complete, as in the case of semitendine and gracile.
  • Another advantage of creating this ligament repair method is when the lesion is recurrent in the individual, so that all possible grafts have already been removed for its treatment or it is a complex lesion involving more than one joint structure.
  • the disadvantages of the method is the need for two surgical times before the reconstruction of injured ligaments or tendons, since the membrane itself is friable and easily ruptured in its handling. Therefore, it is necessary in these cases that there is an action of external forces acting on the joint or structure of the limb, the previous preparation of the material at a suitable site that can expect the metaplasia of the pope-filled membrane in resistant connective tissue.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Molecular Biology (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Vascular Medicine (AREA)
  • Mycology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Rheumatology (AREA)
  • Rehabilitation Therapy (AREA)
  • Biotechnology (AREA)
  • Materials For Medical Uses (AREA)
  • Prostheses (AREA)

Abstract

La présente invention concerne l'utilisation d'une membrane de cellulose bactérienne, trouvant une application dans la reconstruction des ligaments, des tendons et des capsules synoviales conformément aux procédés présentés dans la description technique de l'invention. Cette matière trouverait une application dans la reconstruction de ligaments et tendons (ligaments croisés du genou, ligament rotulien, tendon d'Achille, tendon quadricipital, etc.) et de la capsule synoviale.
PCT/BR2012/000179 2011-06-10 2012-06-06 Procédé d'utilisation de membrane de cellulose bactérienne intra-articulaire sur surface lésée de rotule Ceased WO2012167341A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRPI1102869-6A BRPI1102869A2 (pt) 2011-06-10 2011-06-10 Método de utilização da membrana de celulose bacteriana intra articular sobre superfície lesada de patela
BRPI1102869-6 2011-06-10

Publications (1)

Publication Number Publication Date
WO2012167341A1 true WO2012167341A1 (fr) 2012-12-13

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PCT/BR2012/000179 Ceased WO2012167341A1 (fr) 2011-06-10 2012-06-06 Procédé d'utilisation de membrane de cellulose bactérienne intra-articulaire sur surface lésée de rotule

Country Status (2)

Country Link
BR (1) BRPI1102869A2 (fr)
WO (1) WO2012167341A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105963777A (zh) * 2016-07-08 2016-09-28 福建师范大学 一种细菌纤维素膜组织工程化人工肌腱支架的制备方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116492518B (zh) * 2023-05-12 2024-08-16 四川大学 一种功能等级双层膜及其制备方法与应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003020191A1 (fr) * 2001-09-04 2003-03-13 University Of Iowa Research Foundation Membranes a base de celluloses pour echafaudages biodegradables
EP1410811A1 (fr) * 2002-10-18 2004-04-21 Ethicon, Inc. Support biocompatible comprenant des morceaux de tissu
EP2070557A1 (fr) * 2007-12-12 2009-06-17 Xylos Corporation Matériaux implantables à base de cellulose microbienne pour la réparation et la régénération de tissus durs
US20100159046A1 (en) * 2008-12-19 2010-06-24 Xylos Corporation Minimal tissue attachment implantable materials
US20100297239A1 (en) * 2008-12-22 2010-11-25 Paul Gatenholm Osseointegrative meniscus and cartilage implants based on beta-glucan nanocomposites
DE102009032216A1 (de) * 2009-07-06 2011-01-20 Aesculap Aktiengesellschaft Spacer-Implantat zur Behandlung von menschlichen oder tierischen Gelenkknorpelschäden

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003020191A1 (fr) * 2001-09-04 2003-03-13 University Of Iowa Research Foundation Membranes a base de celluloses pour echafaudages biodegradables
EP1410811A1 (fr) * 2002-10-18 2004-04-21 Ethicon, Inc. Support biocompatible comprenant des morceaux de tissu
EP2070557A1 (fr) * 2007-12-12 2009-06-17 Xylos Corporation Matériaux implantables à base de cellulose microbienne pour la réparation et la régénération de tissus durs
US20100159046A1 (en) * 2008-12-19 2010-06-24 Xylos Corporation Minimal tissue attachment implantable materials
US20100297239A1 (en) * 2008-12-22 2010-11-25 Paul Gatenholm Osseointegrative meniscus and cartilage implants based on beta-glucan nanocomposites
DE102009032216A1 (de) * 2009-07-06 2011-01-20 Aesculap Aktiengesellschaft Spacer-Implantat zur Behandlung von menschlichen oder tierischen Gelenkknorpelschäden

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ABDALLA, C.M. ET AL.: "Uso da membrana de celulose bacteriana associado a células mesenquimais e ácido hialurônico em superficie patelar lesada de suinos.", 13°. CONGRESSO BRASILEIRO DE CIRURGIA DE JOELHO., 27 March 2010 (2010-03-27), pages 1163 *
ABDALLA, C.M.: "Uso da membrana de Celulose Bacteriana", DISSERTAÇÃO DE MESTRADO., 30 November 2010 (2010-11-30) *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105963777A (zh) * 2016-07-08 2016-09-28 福建师范大学 一种细菌纤维素膜组织工程化人工肌腱支架的制备方法

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