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WO2012162049A3 - Methods and compositions for measuring radiation exposure in a subject - Google Patents

Methods and compositions for measuring radiation exposure in a subject Download PDF

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Publication number
WO2012162049A3
WO2012162049A3 PCT/US2012/038077 US2012038077W WO2012162049A3 WO 2012162049 A3 WO2012162049 A3 WO 2012162049A3 US 2012038077 W US2012038077 W US 2012038077W WO 2012162049 A3 WO2012162049 A3 WO 2012162049A3
Authority
WO
WIPO (PCT)
Prior art keywords
ionizing radiation
expression level
biological sample
biomarker
exposed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/038077
Other languages
French (fr)
Other versions
WO2012162049A2 (en
Inventor
Derek L. Stirewalt
Era POGOSOVA-AGADJANYAN
Jerald P. Radich
Wenhong Fan
Bret HELTON
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fred Hutchinson Cancer Center
Original Assignee
Fred Hutchinson Cancer Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fred Hutchinson Cancer Center filed Critical Fred Hutchinson Cancer Center
Publication of WO2012162049A2 publication Critical patent/WO2012162049A2/en
Publication of WO2012162049A3 publication Critical patent/WO2012162049A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present disclosure provides a method for assessing exposure to ionizing radiation comprising the steps of (a) measuring the RNA expression level of certain biomarker genes in a biological sample; (b) correcting the RNA expression level of the measured biomarker in step (a) to a reference standard or threshold value, and (c) comparing the RNA expression level of the biomarker measured in step (a) and corrected in accordance with step (b) to the corrected expression of a calibration sample or threshold value, wherein a difference in expression level between the biomarker in the biological sample and the calibration sample indicates that the source from which the biological sample is obtained was exposed to ionizing radiation. The method can further comprise classifying the source of the biological sample as either exposed or not exposed to ionizing radiation. Kits for assessing exposure to ionizing radiation are also described.
PCT/US2012/038077 2011-05-20 2012-05-16 Methods and compositions for measuring radiation exposure in a subject Ceased WO2012162049A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161488631P 2011-05-20 2011-05-20
US61/488,631 2011-05-20

Publications (2)

Publication Number Publication Date
WO2012162049A2 WO2012162049A2 (en) 2012-11-29
WO2012162049A3 true WO2012162049A3 (en) 2013-03-07

Family

ID=47217975

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/038077 Ceased WO2012162049A2 (en) 2011-05-20 2012-05-16 Methods and compositions for measuring radiation exposure in a subject

Country Status (1)

Country Link
WO (1) WO2012162049A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110564864A (en) * 2019-09-24 2019-12-13 中国人民解放军军事科学院军事医学研究院 Application of miR-6090 as gamma-ray radiation marker
CN111621559A (en) * 2020-06-11 2020-09-04 北京市化工职业病防治院(北京市职业病防治研究院) Screening of Low dose ionizing radiation Effector genes in Association of transcriptome and proteome data
CN114150054B (en) * 2021-12-03 2022-08-02 中国科学院近代物理研究所 Reagents for detecting or assessing ionizing radiation damage or exposure and tRNA-derived fragments thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008030278A2 (en) * 2006-06-05 2008-03-13 Fred Hutchinson Cancer Research Center Assays for radiation exposure
WO2011006214A1 (en) * 2009-07-16 2011-01-20 Peter Maccallum Cancer Institute Method of detecting radiation exposure and adverse toxicity thereto

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008030278A2 (en) * 2006-06-05 2008-03-13 Fred Hutchinson Cancer Research Center Assays for radiation exposure
WO2011006214A1 (en) * 2009-07-16 2011-01-20 Peter Maccallum Cancer Institute Method of detecting radiation exposure and adverse toxicity thereto

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GROSOVSKY, A. J. ET AL.: "Base substitutions, frameshifts, and small deletions constitute ionizing radiation-induced point mutations in mammalian cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 85, no. 1, January 1988 (1988-01-01), pages 185 - 188 *
LEE, J. H. ET AL.: "Identification of a novel ionizing radiation-induced nuclease, AEN, and its functional characterization in apoptosis", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 337, no. 1, 11 November 2005 (2005-11-11), pages 39 - 47 *
POGOSOVA-AGADJANYAN, E. L. ET AL.: "Identification of radiation-induced expression changes in nonimmortalized human T cells", RADIATION RESEARCH, vol. 175, no. 2, February 2011 (2011-02-01), pages 172 - 184 *

Also Published As

Publication number Publication date
WO2012162049A2 (en) 2012-11-29

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