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WO2012027374A3 - Novel fungal carbohydrate hydrolases - Google Patents

Novel fungal carbohydrate hydrolases Download PDF

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Publication number
WO2012027374A3
WO2012027374A3 PCT/US2011/048817 US2011048817W WO2012027374A3 WO 2012027374 A3 WO2012027374 A3 WO 2012027374A3 US 2011048817 W US2011048817 W US 2011048817W WO 2012027374 A3 WO2012027374 A3 WO 2012027374A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzymes
relates
novel
methods
compositions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/048817
Other languages
French (fr)
Other versions
WO2012027374A2 (en
Inventor
Johannes Visser
Sandra Hinz
Jan Werij
Jacob Visser
Vivi Joosten
Martijn Koetsier
Mark Emalfarb
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dyadic International USA Inc
Original Assignee
Dyadic International USA Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dyadic International USA Inc filed Critical Dyadic International USA Inc
Publication of WO2012027374A2 publication Critical patent/WO2012027374A2/en
Publication of WO2012027374A3 publication Critical patent/WO2012027374A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01055—Alpha-N-arabinofuranosidase (3.2.1.55)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)

Abstract

This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes to improve digestability of animal feed and in a variety of other processes, including food and beverage processing, baking, washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.
PCT/US2011/048817 2010-08-23 2011-08-23 Novel fungal carbohydrate hydrolases Ceased WO2012027374A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37609210P 2010-08-23 2010-08-23
US61/376,092 2010-08-23

Publications (2)

Publication Number Publication Date
WO2012027374A2 WO2012027374A2 (en) 2012-03-01
WO2012027374A3 true WO2012027374A3 (en) 2012-08-16

Family

ID=45724021

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/048817 Ceased WO2012027374A2 (en) 2010-08-23 2011-08-23 Novel fungal carbohydrate hydrolases

Country Status (1)

Country Link
WO (1) WO2012027374A2 (en)

Families Citing this family (33)

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EP2606131B1 (en) * 2010-08-20 2017-03-08 Codexis, Inc. Use of glycoside hydrolase 61 family proteins in processing of cellulose
US20130288310A1 (en) * 2010-08-23 2013-10-31 Codexis, Inc Recombinant lignocellulose degradation enzymes for the production of soluble sugars from cellulosic biomass
EP2780450A4 (en) * 2011-11-15 2015-09-09 Novozymes Inc Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2013096369A1 (en) 2011-12-19 2013-06-27 Novozymes A/S Processes and compositions for increasing the digestibility of cellulosic materials
BR112014030463A2 (en) * 2012-06-08 2017-06-27 Concordia Univ innovative enzymes for cell wall deconstruction of scytalidium thermophilum, myriococcum thermophilum and aureobasidium pullulans and their uses
WO2013182669A2 (en) * 2012-06-08 2013-12-12 Dsm Ip Assets B.V. Novel cell wall deconstruction enzymes of myriococcum thermophilum and uses thereof
WO2013182670A2 (en) * 2012-06-08 2013-12-12 Dsm Ip Assets B.V. Novel cell wall deconstruction enzymes of scytalidium thermophilum and uses thereof
WO2013188305A2 (en) * 2012-06-11 2013-12-19 Codexis, Inc. Fungal beta-xylosidase variants
KR101535526B1 (en) * 2012-06-26 2015-07-09 한국생명공학연구원 Fusion proteins of GH61 with enhanced secretory capability comparing to its wild-type
WO2014005499A1 (en) * 2012-07-02 2014-01-09 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
EP2772545A1 (en) * 2013-03-01 2014-09-03 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Starch active proteins
EP3074513A1 (en) 2013-11-26 2016-10-05 Novozymes A/S Enzyme compositions and uses thereof
DK3152315T3 (en) 2014-06-06 2018-11-26 Novozymes As ENZYME COMPOSITIONS AND APPLICATIONS THEREOF
CA2954974A1 (en) 2014-07-21 2016-01-28 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
US20180051306A1 (en) 2015-03-12 2018-02-22 Novozymes A/S Enzymatic Hydrolysis with Hemicellulolytic Enzymes
WO2016145363A1 (en) 2015-03-12 2016-09-15 Novozymes A/S Multi-stage enzymatic hydrolysis of lignocellulosic biomass employing an oxidoreductase with an aa9 polypeptide
EP3268484B1 (en) 2015-03-12 2020-06-17 Novozymes A/S Multi-stage enzymatic hydrolysis of lignocellulosic biomass
CA2981342A1 (en) 2015-06-18 2016-12-22 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
BR112019012802A2 (en) 2016-12-21 2019-12-03 Novozymes As gh25 isolated polypeptide, methods for hydrolyzing peptidoglycan in bacterial cell walls, increasing the digestibility of peptidoglycans in animal feed, producing the polypeptide, improving intestinal health in an animal and promoting the elimination of dead lactobacillus johnsonii cells animal feed, animal feed, composition, polynucleotide, recombinant host cell, use of the polypeptide, and zootechnical additive for use in feed
CN110072996A (en) 2016-12-21 2019-07-30 诺维信公司 Polypeptide with lysozyme activity, the polynucleotides for encoding it and application thereof and composition
CA3048773A1 (en) 2017-01-04 2018-07-12 Novozymes A/S Microbial lysozyme for use in the treatment of irritable bowel syndrome or inflammatory bowel disease
MX2019014889A (en) 2017-06-28 2020-02-13 Novozymes As Polypeptides having trehalase activity and polynucleotides encoding same.
CN111094562A (en) 2017-08-08 2020-05-01 诺维信公司 Polypeptides with trehalase activity and their use in methods for producing fermentation products
EP3467172A1 (en) 2017-10-06 2019-04-10 Lenzing Aktiengesellschaft Silk-like woven garment containing or consisting of lyocell filaments
EP3467171A1 (en) * 2017-10-06 2019-04-10 Lenzing Aktiengesellschaft Lyocell filament denim
CN108004153B (en) * 2017-12-07 2021-05-28 潍坊康地恩生物科技有限公司 Trichoderma reesei strain capable of producing pectin lyase in high yield and application thereof
CN108265092B (en) * 2017-12-26 2021-07-27 天津大学 A kind of mushroom oligosaccharide with excellent antioxidant activity and preparation method thereof
BR112020024347A2 (en) 2018-05-31 2021-02-23 Novozymes A/S processes to increase yeast growth and productivity
CN112272671A (en) * 2018-06-01 2021-01-26 诺维信公司 Polypeptides
EP4291634A4 (en) * 2021-02-10 2025-01-01 Novozymes A/S Polypeptides having pectinase activity, polynucleotides encoding same, and uses thereof
GB2610716A (en) * 2021-03-30 2023-03-15 Glycomics And Glycan Bioengineering Res Center Process for the direct enzymatic conversion of amino sugars; enzyme and compositions for use in the process
AU2023272468A1 (en) 2022-05-14 2024-11-14 Novonesis Plant Biosolutions A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
EP4547210A1 (en) * 2022-06-30 2025-05-07 Novozymes A/S Mutanases and oral care compositions comprising same

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WO2008151079A2 (en) * 2007-05-31 2008-12-11 Novozymes, Inc. Compositions for degrading cellulosic material
US20100003234A1 (en) * 2005-03-15 2010-01-07 Verenium Corporation Cellulases, Nucleic Acids Encoding Them and Methods for Making and Using Them

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060185036A1 (en) * 2000-10-20 2006-08-17 Sticklen Masomeh B Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
US20100003234A1 (en) * 2005-03-15 2010-01-07 Verenium Corporation Cellulases, Nucleic Acids Encoding Them and Methods for Making and Using Them
WO2008151079A2 (en) * 2007-05-31 2008-12-11 Novozymes, Inc. Compositions for degrading cellulosic material

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CLAUDIA GROPOSO ET AL.: "Contribution to the lignocellulolytic fungi (Basidiomycetes) of the Atlantic Rain Forest in Southern Brazil.", MYCOTAXON., vol. 92, 2005, pages 103 - 113 *
DATABASE GENBANK 9 April 2008 (2008-04-09), accession no. M_001228634.1 *
DATABASE GENBANK 9 April 2008 (2008-04-09), accession no. P_001228635.1 *

Also Published As

Publication number Publication date
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