[go: up one dir, main page]

WO2012009272A3 - Pentose fermentation by a recombinant microorganism - Google Patents

Pentose fermentation by a recombinant microorganism Download PDF

Info

Publication number
WO2012009272A3
WO2012009272A3 PCT/US2011/043548 US2011043548W WO2012009272A3 WO 2012009272 A3 WO2012009272 A3 WO 2012009272A3 US 2011043548 W US2011043548 W US 2011043548W WO 2012009272 A3 WO2012009272 A3 WO 2012009272A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant microorganism
pentose fermentation
recombinant
xylose isomerase
pentose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/043548
Other languages
French (fr)
Other versions
WO2012009272A2 (en
Inventor
Ezhilkani Subbian
Xiyun Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Codexis Inc
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of WO2012009272A2 publication Critical patent/WO2012009272A2/en
Publication of WO2012009272A3 publication Critical patent/WO2012009272A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.
PCT/US2011/043548 2010-07-14 2011-07-11 Pentose fermentation by a recombinant microorganism Ceased WO2012009272A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36418010P 2010-07-14 2010-07-14
US61/364,180 2010-07-14

Publications (2)

Publication Number Publication Date
WO2012009272A2 WO2012009272A2 (en) 2012-01-19
WO2012009272A3 true WO2012009272A3 (en) 2012-03-01

Family

ID=45470018

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/043548 Ceased WO2012009272A2 (en) 2010-07-14 2011-07-11 Pentose fermentation by a recombinant microorganism

Country Status (1)

Country Link
WO (1) WO2012009272A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2626423A1 (en) * 2012-02-07 2013-08-14 Clariant Produkte (Deutschland) GmbH Pentose-fermenting microorganism
US8669076B1 (en) 2013-03-11 2014-03-11 E I Du Pont De Nemours And Company Cow rumen xylose isomerases active in yeast cells
US9187743B2 (en) 2013-03-11 2015-11-17 E I Du Pont De Nemours And Company Bacterial xylose isomerases active in yeast cells
AU2016293487B2 (en) 2015-07-13 2021-07-01 MARA Renewables Corporation Enhancing microalgal metabolism of xylose
BR112019011612A2 (en) 2016-12-06 2020-08-18 Novozymes A/S improved processes for the production of ethanol from cellulosic substrates containing xylose using genetically modified yeast strains
WO2018114973A1 (en) 2016-12-20 2018-06-28 Novozymes A/S Recombinant yeast strains for pentose fermentation
EP3630989A1 (en) 2017-06-02 2020-04-08 Novozymes A/S Improved yeast for ethanol production
AU2019213033A1 (en) 2018-01-29 2020-09-17 Microbiogen Pty. Ltd. Microorganisms with improved nitrogen utilization for ethanol production
AU2019309683A1 (en) 2018-07-25 2021-02-11 Novozymes A/S Enzyme-expressing yeast for ethanol production
CA3114783A1 (en) 2018-10-08 2020-04-16 Novozymes A/S Enzyme-expressing yeast for ethanol production
MX2021015821A (en) 2019-07-26 2022-02-03 Novozymes As Microorganisms with improved nitrogen transport for ethanol production.
CN114127124A (en) 2019-08-06 2022-03-01 诺维信公司 Fusion proteins for enhanced enzyme expression
CN115175923A (en) 2019-12-10 2022-10-11 诺维信公司 Microorganisms for improved pentose fermentation
CN117795089A (en) 2021-06-07 2024-03-29 诺维信公司 Engineered microorganisms for improved ethanol fermentation
WO2024258820A2 (en) 2023-06-13 2024-12-19 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010000464A1 (en) * 2008-07-02 2010-01-07 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotic xylose isomerase for the construction of xylose fermenting yeasts
US20100086965A1 (en) * 2006-10-02 2010-04-08 Van Maris Antonius Jeroen Adriaan Metabolic engineering of arabinose-fermenting yeast cells
US20100129885A1 (en) * 2007-10-26 2010-05-27 Arbor Fuel Inc. Methods for the production of n-butanol

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100086965A1 (en) * 2006-10-02 2010-04-08 Van Maris Antonius Jeroen Adriaan Metabolic engineering of arabinose-fermenting yeast cells
US20100129885A1 (en) * 2007-10-26 2010-05-27 Arbor Fuel Inc. Methods for the production of n-butanol
WO2010000464A1 (en) * 2008-07-02 2010-01-07 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotic xylose isomerase for the construction of xylose fermenting yeasts

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BRAT ET AL.: "Functional Expression of a Bacterial Xylose Isomerase in Saccharomyces cerevisiae.", APPL. ENV. MICRO., vol. 75, no. 8, April 2009 (2009-04-01), pages 2304 - 2311, XP009121860, DOI: doi:10.1128/AEM.02522-08 *
DATABASE UNIPROTKB/TREMBL [online] 7 July 2009 (2009-07-07), WEINSTOCK ET AL., retrieved from http://www.uniprot.org/uniprot/C4G751 .txt?version=8 Database accession no. C4G751 *

Also Published As

Publication number Publication date
WO2012009272A2 (en) 2012-01-19

Similar Documents

Publication Publication Date Title
WO2012009272A3 (en) Pentose fermentation by a recombinant microorganism
EP2576804A4 (en) Pentose fermentation by a recombinant microorganism
WO2011082425A3 (en) Alpha-amylase variants and polynucleotides encoding same
WO2012044915A3 (en) Beta-glucosidase variants and polynucleotides encoding same
WO2013057141A3 (en) Alpha-amylase variants and polynucleotides encoding same
WO2013057143A3 (en) Alpha-amylase variants and polynucleotides encoding same
WO2013006756A3 (en) Alpha amylase variants and polynucleotides encoding same
WO2013001087A3 (en) Method for screening alpha-amylases
WO2012103300A3 (en) Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012146171A9 (en) Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2013019827A3 (en) Polypeptides having xylanase activity and polynucleotides encoding same
WO2011109524A3 (en) Xylanase variants and polynucleotides encoding same
HK1207111A1 (en) Methods and compositions for controlling gene expression by rna processing
MX353621B (en) Alpha-amylase variants.
MX351762B (en) Glucoamylase variants and polynucleotides encoding same.
WO2013076259A3 (en) Polypeptides having lysozyme activity and polynucleotides encoding same
WO2011035027A3 (en) Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2011005867A8 (en) Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP2735611A3 (en) Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
MX2012011774A (en) Polypeptides having glucoamylase activity and polynucleotides encoding same.
WO2009127702A3 (en) Laccase variants
WO2013119302A3 (en) Gh61 polypeptide variants and polynucleotides encoding same
WO2013096603A3 (en) Cellobiohydrolase variants and polynucleotides encoding same
WO2011153276A3 (en) Stable, functional chimeric cellobiohydrolase class i enzymes
WO2013074956A3 (en) Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11807348

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11807348

Country of ref document: EP

Kind code of ref document: A2