[go: up one dir, main page]

WO2012008813A3 - 양막유래 중간엽 줄기세포 배양을 위한 배지조성물 및 이를 이용한 양막유래 중간엽 줄기세포의 배양방법 - Google Patents

양막유래 중간엽 줄기세포 배양을 위한 배지조성물 및 이를 이용한 양막유래 중간엽 줄기세포의 배양방법 Download PDF

Info

Publication number
WO2012008813A3
WO2012008813A3 PCT/KR2011/005258 KR2011005258W WO2012008813A3 WO 2012008813 A3 WO2012008813 A3 WO 2012008813A3 KR 2011005258 W KR2011005258 W KR 2011005258W WO 2012008813 A3 WO2012008813 A3 WO 2012008813A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cell
mesenchymal stem
culturing
culture medium
derived mesenchymal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2011/005258
Other languages
English (en)
French (fr)
Other versions
WO2012008813A2 (ko
Inventor
라정찬
강성근
서주연
김효은
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RNL Bio Co Ltd
Original Assignee
RNL Bio Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RNL Bio Co Ltd filed Critical RNL Bio Co Ltd
Priority to AU2011277156A priority Critical patent/AU2011277156B2/en
Priority to ES11807097.8T priority patent/ES2527253T3/es
Priority to BR112013001089A priority patent/BR112013001089A2/pt
Priority to US13/810,334 priority patent/US8871513B2/en
Priority to SG2013003777A priority patent/SG187114A1/en
Priority to CN201180038199.1A priority patent/CN103154241B/zh
Priority to EP11807097.8A priority patent/EP2594636B1/en
Priority to JP2013520643A priority patent/JP5750159B2/ja
Publication of WO2012008813A2 publication Critical patent/WO2012008813A2/ko
Publication of WO2012008813A3 publication Critical patent/WO2012008813A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Rheumatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 중간엽 줄기세포 배양을 위한 배양배지에 관한 것으로, 더욱 자세하게는 기본 배지, L-아스코르브산 2-인산, 우태아혈청, 염기성 섬유아세포 성장인자(b-FGF), 비필수 아미노산(NEAA; non essential amino acid), 인슐린, N-아세틸-L-시스테인(N-acetyl-L-cysteine), 칼슘클로라이드 및 하이드로코티손을 함유하는 중간엽 줄기세포 배양을 위한 배지조성물 및 이를 이용한 중간엽 줄기세포의 배양방법에 관한 것이다. 본 발명에 따르면, 줄기세포 치료에 필요한 중간엽 줄기세포의 개체수를 빠른 시간에 획득할 수 있으며, 중간엽 줄기세포의 분화능을 향상시켜, 줄기세포를 이용한 세포치료에 유용하다.
PCT/KR2011/005258 2010-07-16 2011-07-18 양막유래 중간엽 줄기세포 배양을 위한 배지조성물 및 이를 이용한 양막유래 중간엽 줄기세포의 배양방법 Ceased WO2012008813A2 (ko)

Priority Applications (8)

Application Number Priority Date Filing Date Title
AU2011277156A AU2011277156B2 (en) 2010-07-16 2011-07-18 Culture medium composition for culturing amnion-derived mesenchymal stem cell, and method for culturing amnion-derived mesenchymal stem cell by using same
ES11807097.8T ES2527253T3 (es) 2010-07-16 2011-07-18 Composición de medio de cultivo para cultivar células madre mesenquimales derivadas del amnios y método para cultivar células madre mesenquimales derivadas del amnios utilizando la misma
BR112013001089A BR112013001089A2 (pt) 2010-07-16 2011-07-18 composição de meio de cultura e método para cultivar células tronco mesenquimais derivadas de âmnio
US13/810,334 US8871513B2 (en) 2010-07-16 2011-07-18 Culture medium composition for culturing amnion-derived mesenchymal stem cell, and method for culturing amnion-derived mesenchymal stem cell by using same
SG2013003777A SG187114A1 (en) 2010-07-16 2011-07-18 Culture medium composition for culturing amnion-derived mesenchymal stem cell, and method for culturing amnion-derived mesenchymal stem cell by using same
CN201180038199.1A CN103154241B (zh) 2010-07-16 2011-07-18 用于培养羊膜来源间充质干细胞的培养基组合物和用其培养羊膜来源间充质干细胞的方法
EP11807097.8A EP2594636B1 (en) 2010-07-16 2011-07-18 Culture medium composition for culturing amnion-derived mesenchymal stem cell, and method for culturing amnion-derived mesenchymal stem cell by using same
JP2013520643A JP5750159B2 (ja) 2010-07-16 2011-07-18 羊膜由来間葉幹細胞培養用の培地組成物及びこれを利用した羊膜由来間葉幹細胞の培養方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0068959 2010-07-16
KR1020100068959A KR101211913B1 (ko) 2010-07-16 2010-07-16 양막유래 중간엽 줄기세포 배양을 위한 배지조성물 및 이를 이용한 양막유래 중간엽 줄기세포의 배양방법

Publications (2)

Publication Number Publication Date
WO2012008813A2 WO2012008813A2 (ko) 2012-01-19
WO2012008813A3 true WO2012008813A3 (ko) 2012-05-03

Family

ID=45469969

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/005258 Ceased WO2012008813A2 (ko) 2010-07-16 2011-07-18 양막유래 중간엽 줄기세포 배양을 위한 배지조성물 및 이를 이용한 양막유래 중간엽 줄기세포의 배양방법

Country Status (10)

Country Link
US (1) US8871513B2 (ko)
EP (1) EP2594636B1 (ko)
JP (1) JP5750159B2 (ko)
KR (1) KR101211913B1 (ko)
CN (1) CN103154241B (ko)
AU (1) AU2011277156B2 (ko)
BR (1) BR112013001089A2 (ko)
ES (1) ES2527253T3 (ko)
SG (1) SG187114A1 (ko)
WO (1) WO2012008813A2 (ko)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101382566B1 (ko) * 2012-02-01 2014-04-10 동아대학교 산학협력단 태반 양막 유래 중간엽 줄기세포의 배양방법 및 말초 동맥 질환 치료법 및 조성물
WO2013157850A1 (ko) * 2012-04-18 2013-10-24 Ra Jeong Chan 혈관 내 투여에 적합한 크기를 가지는 줄기세포의 제조방법
KR101523040B1 (ko) * 2012-06-26 2015-05-26 라정찬 고농도의 줄기세포 제조방법
KR20140038236A (ko) * 2012-09-20 2014-03-28 메디포스트(주) 간엽줄기세포 응집체의 제조 방법
CN103333856B (zh) * 2013-07-25 2015-08-26 张潇潇 人脐带间充质干细胞培养基
CN104371970B (zh) * 2013-08-15 2017-08-04 协和华东干细胞基因工程有限公司 一种用于培养人羊膜上皮细胞的培养基
CN104450608B (zh) * 2013-09-12 2017-10-24 佛教慈济医疗财团法人 促人类间质干细胞增生的佐剂、扩增人类间质干细胞方法、医药组合物、生长因子及其用途
CN103966159B (zh) * 2014-02-13 2015-08-05 天津和泽干细胞科技有限公司 人胎盘亚全能干细胞及其干细胞库构建方法
EP3140417B1 (en) * 2014-05-09 2021-04-21 Reelabs Private Limited Foetal polymix of mesenchymal stem cells under hypoxic conditions for the treatment of clinical disorders
EP3184627B1 (en) 2014-08-21 2020-12-23 Ajinomoto Co., Inc. Culture medium for mesenchymal stem cells
SG11201703512QA (en) * 2014-10-29 2017-06-29 R Bio Co Ltd Medium composition for culturing stem cells
CN106032527B (zh) * 2015-03-17 2019-08-13 广州市搏克肿瘤研究所 一种耐受低密度的无饲养层人多能干细胞培养基
CN105176912B (zh) * 2015-08-28 2019-02-22 广州赛莱拉干细胞科技股份有限公司 一种培养基及其应用、制备方法
KR101779932B1 (ko) * 2015-09-15 2017-09-21 주식회사 스템랩 나노그(Nanog)를 도입한 양수 내 태아 유래 중간엽 줄기세포를 이용한 육모 촉진용 조성물의 제조방법
CN105420184A (zh) * 2015-12-11 2016-03-23 郭镭 一种从脐带外层羊膜组织中分离培养脐带间充质干细胞的方法
CN105754936B (zh) * 2016-02-25 2018-06-08 付清玲 人诱导性多能干细胞诱导为间充质干细胞的方法
US10894947B1 (en) * 2016-04-29 2021-01-19 Hope Biosciences, Llc Method for generating protein rich conditioned medium
US10959425B2 (en) * 2016-04-29 2021-03-30 Hope Biosciences, Llc Method of banking stem cells
US11111480B2 (en) * 2016-04-29 2021-09-07 Hope Biosctences, Llc Culture media for multipotent stem cells
CN110484500A (zh) * 2019-09-23 2019-11-22 上海亚载生物科技有限公司 一种分离和扩增人尿源间充质干细胞的方法
JP7348892B2 (ja) * 2019-12-30 2023-09-21 佛教慈濟醫療財團法人 眼疾患を予防または治療する組成物及び方法
GB2596216A (en) * 2021-05-09 2021-12-22 Zhejiang Legou Tech Co Ltd An elliptical trainer with protective function
KR20250023295A (ko) 2023-08-08 2025-02-18 주식회사 현대메디텍 N-아세틸시스테인(nac)을 이용한 편도 유래 중간엽 줄기세포의 배양방법

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080152629A1 (en) * 2000-12-06 2008-06-26 James Edinger Placental stem cell populations
US20050260748A1 (en) * 2004-02-27 2005-11-24 Michigan State University Adult stem cells and uses thereof
NZ553695A (en) 2004-08-16 2009-10-30 Cellres Corp Pte Ltd Isolation of stem/progenitor cells from amniotic membrane of umbilical cord
KR100679642B1 (ko) * 2005-11-16 2007-02-06 주식회사 알앤엘바이오 인간 지방조직 유래 다분화능 줄기세포 및 이를 함유하는세포치료제
DK2471904T3 (en) * 2005-12-29 2019-02-18 Celularity Inc Placenta stem cell populations
KR100818214B1 (ko) 2006-09-29 2008-04-01 재단법인서울대학교산학협력재단 인간 태반조직의 양막 또는 탈락막 유래 다분화능 줄기세포및 그 제조방법
US20080159999A1 (en) * 2006-10-23 2008-07-03 Konstantinos Stefanidis Compositions and methods for identifying, isolating and enriching germline-like stem cells from amniotic fluid
KR100795708B1 (ko) 2006-12-26 2008-01-17 주식회사 알앤엘바이오 인간 양막 상피세포 유래 성체 줄기세포의 분리 및 배양방법
WO2009061024A1 (en) 2007-11-09 2009-05-14 Rnl Bio Co., Ltd Method for isolating and culturing adult stem cells derived from human amniotic epithelium

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AHN, H-J. ET. AL. ET AL.: "FGF2 stimulates the proliferation of human mesenchymal stem cells through the transient activation of JNK signaling", FEBS LETTERS, vol. 583, 5 August 2009 (2009-08-05), pages 2922 - 2926, XP026542699 *
BATTULA, V.L. ET AL.: "Human placenta and bone marrow derived MSC cultured in serum-free, b-FGF-containing medium express cell surface frizzled-9 and SSEA-4 and give rise to multilineage differentiation", DIFFERENTIATION, vol. 75, no. 4, April 2007 (2007-04-01), pages 279 - 291, XP002457620 *
LIN, T-M. ET AL.: "Accelerated growth and prolonged lifespan of adipose tissue-derived human mesenchymal stem cells in a medium using reduced calcium and antioxidants", STEM CELLS AND DEVELOPMENT, vol. 14, 2005, pages 92 - 102, XP009046469 *
LIU, C-H. ET AL.: "Cytokine interactions in mesenchymal stem cells from cord blood", CYTOKINE, vol. 32, 2005, pages 270 - 279, XP005265995 *
MEULEMAN, N. ET AL.: "Human marrow mesenchymal stem cell culture: serum-free medium allows better expansion than classical a-MEM medium'", EUROPEAN JOURNAL OF HAEMATOLOGY, vol. 76, 2006, pages 309 - 316, XP055107919 *
See also references of EP2594636A4 *

Also Published As

Publication number Publication date
BR112013001089A2 (pt) 2019-09-24
CN103154241A (zh) 2013-06-12
EP2594636B1 (en) 2014-11-26
ES2527253T3 (es) 2015-01-21
EP2594636A2 (en) 2013-05-22
AU2011277156B2 (en) 2014-06-05
US8871513B2 (en) 2014-10-28
WO2012008813A2 (ko) 2012-01-19
CN103154241B (zh) 2015-05-20
AU2011277156A1 (en) 2013-02-07
KR20120008223A (ko) 2012-01-30
JP2013530721A (ja) 2013-08-01
US20130164849A1 (en) 2013-06-27
JP5750159B2 (ja) 2015-07-15
SG187114A1 (en) 2013-02-28
KR101211913B1 (ko) 2012-12-13
EP2594636A4 (en) 2014-01-22

Similar Documents

Publication Publication Date Title
WO2012008813A3 (ko) 양막유래 중간엽 줄기세포 배양을 위한 배지조성물 및 이를 이용한 양막유래 중간엽 줄기세포의 배양방법
WO2011159359A3 (en) Serum-free chemically defined cell culture medium
MX2012003654A (es) Metodos de produccion de glucoproteinas en cultivos de celulas de mamiferos usando glucocorticoides.
EP4368701A3 (en) Feeder-free culture system
Sart et al. Process engineering of stem cell metabolism for large scale expansion and differentiation in bioreactors
WO2012052854A3 (en) Plant cell lines and methods of isolating the same
NZ598677A (en) Production of proteins in glutamine-free cell culture media
WO2008114721A1 (ja) Ｌ－グルタミン酸系アミノ酸生産微生物及びアミノ酸の製造法
WO2010147395A3 (en) Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same
WO2009023525A3 (en) Methods of modulating mesenchymal stem cell differentiation
WO2008154014A3 (en) A method for culturing mammalian cells to improve recombinant protein production
MY169476A (en) A method for increasing photosynthetic carbon fixation using glycolate dehydrogenase multi-subunit fusion protein
WO2009145419A9 (ko) 식물성 펩톤을 포함하는 줄기세포 증식 촉진용 조성물
MY178988A (en) Process for the fermentative preparation of sulphurous amino acids
US20250270512A1 (en) Additive for undifferentiation maintaining medium
IN2012DN02964A (ko)
WO2012141471A3 (ko) 역분화-증진제를 이용한 역분화 만능 줄기세포의 제조방법
UA92157C2 (ru) Способ продуцирования гормона роста
Tarle et al. Development of a serum‐free system to expand dental‐derived stem cells: PDLSCs and SHEDs
WO2011106681A3 (en) SMOOTH MUSCLE-LIKE CELLS (SMLCs) DERIVED FROM HUMAN PLURIPOTENT STEM CELLS
WO2011105821A3 (ko) 무혈청 줄기 세포 배양 조성물, 이를 함유한 조직 재생용 조성물 및 이를 이용한 조직 재생 방법
JP2013500003A5 (ko)
WO2011065661A3 (ko) 중간엽 줄기세포를 dkk-1 또는 sfrp-1을 이용하여 연골세포로 분화시키는 방법
CA2865497A1 (en) Method for guiding the derivation of endothelial cells from human pluripotent stem cells employing two-dimensional, feeder-free differentiation
WO2010002503A3 (en) Electrobiochemical reactor

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 201180038199.1

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11807097

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2011807097

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2013520643

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2011277156

Country of ref document: AU

Date of ref document: 20110718

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 13810334

Country of ref document: US

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112013001089

Country of ref document: BR

ENP Entry into the national phase

Ref document number: 112013001089

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20130116