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WO2012003797A1 - Dispositif de prélèvement d'échantillons liquides - Google Patents

Dispositif de prélèvement d'échantillons liquides Download PDF

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Publication number
WO2012003797A1
WO2012003797A1 PCT/CN2011/076897 CN2011076897W WO2012003797A1 WO 2012003797 A1 WO2012003797 A1 WO 2012003797A1 CN 2011076897 W CN2011076897 W CN 2011076897W WO 2012003797 A1 WO2012003797 A1 WO 2012003797A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample
receiver
way valve
check valve
elastic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2011/076897
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English (en)
Chinese (zh)
Inventor
胡林
胡海鹏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ABON Biopharm Hangzhou Co Ltd
Original Assignee
ABON Biopharm Hangzhou Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ABON Biopharm Hangzhou Co Ltd filed Critical ABON Biopharm Hangzhou Co Ltd
Publication of WO2012003797A1 publication Critical patent/WO2012003797A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices

Definitions

  • the present invention relates to a sample collection device, and more particularly to a collection device consisting of a sample receptacle and a one-way valve.
  • the invention also relates to a method of collecting a sample using such a sample collection device. Background technique
  • U.S. Patent No. 2009/0024058 A1 discloses a liquid sample collection device which is mainly composed of a sample collection box, a collection baffle connected to the collection box, and a one-way valve communicating with the two. The air in the device is drained, but the outside air is prevented from flowing back into the device.
  • the sample collection device discharges the air in the collection vessel through the one-way valve by the pressure of the fluid collected in the collection box, thereby collecting the sample into the collection vessel to complete the collection process.
  • This sample collection device is simple and convenient to operate, and can collect large sample sizes, making it possible to collect samples in batches clinically.
  • this collection device still has some problems: the pressure of the fluid itself causes the sample to flow into the collection vessel, and the amount of liquid collected is not large enough; in addition, some samples may not be returned to the collection box due to insufficient pressure, resulting in sample contamination. Or polluting the sampler.
  • the present invention provides a sample collection device.
  • the collection device includes a sample receiver for holding a sample and two one-way flow check valves connected to the sample receiver. When the device is in operation, the two check valves are not open at the same time, that is, when one check valve is opened, the other check valve is closed.
  • the other check valve closes; and when the other check valve opens, the exhaust or liquid The check valve is closed.
  • the other check valve when one check valve discharges air or liquid to the outside, when the negative pressure is generated in the receiver, the other check valve is closed; when the other check valve is opened, due to the pressure balance Acting, the external gas, liquid sample, or another gas in communication with the other one-way valve enters the sample receiver through the open one-way valve, and the air-discharged check valve is now closed.
  • the sample receptacle can include an opening, and the two one-way valves are coupled to the opening of the sample receiver and are coupled to opposite sides of the open end, respectively.
  • the receiver may include two openings, two of which are connected to the two openings, respectively.
  • the sample receiver described above is for receiving and containing samples collected by the device, and the receiver can be made of any material that meets medical standards.
  • the receiver can be made of a resilient material.
  • a resilient material such as rubber, plastic, PVC and silica gel, etc.; or other elastic materials, and can achieve the following functions: that is, when there is force acting on the receiver, such as squeezing the receiver, the receiver will automatically compress, the receiver The inner space is reduced, forcing the air or sample in the receiver to exit the outside through a one-way valve, at which point the other check valve is closed, thus causing the receiver to form a differential pressure with the outside atmosphere; thereby creating a negative pressure in the receiver.
  • the resulting negative pressure forces a sample of gas or liquid in communication with another one-way valve into the receiver. More preferably, if the receiver is re-squeezed, the collected liquid or other sample is discharged to the outside of the receptacle through the open check valve, and the sample is collected again, so that the liquid or gas sample can be continuously collected.
  • the receiver can also be made of a transparent material.
  • Transparent means a material that is permeable to light so that under normal room light, a person's naked eye can see the object behind the transparent material, and the person using the device can see the sample size under the transparent window. Or test the results.
  • the sample receiver in the device can be replaced and connected to the one-way valve in a detachable manner.
  • the sample is collected into the receiver and the receiver can be removed and replaced with another receiver. This allows batch sampling.
  • the receiver can be used once or repeatedly.
  • the receiver may be removed, the sample may be poured onto the detecting component, or sampled into the receiver and sampled and added to the detecting component for detection; or at the receiver
  • the test element is directly placed in the test element, such as a test strip for testing a component in the saliva, and the test sample is directly contacted with the test component while the sample enters the receiver.
  • the other end of the one-way valve connected to the sample receiver is connectable
  • the dripper when the sample is collected in the receptacle, can be applied to the receiver by applying pressure, such as squeezing the sample receiver with elasticity, so that the pressure inside the receptacle is increased, so that the check valve connected to the dripper is When opened, the sample in the receiver is discharged to the test element through the check valve and the dripper for detection.
  • a lid for sealing can also be mounted on the sample receiver.
  • the receiver When the device has been sampled, the receiver is removed, the lid is closed, and the sample is sealed in the receptacle and will not leak, whether flipped or shaken.
  • a sampling pipette associated with the receiver opening may be designed to seal the receiver for storage of the sample.
  • a dripper device is designed inside the cover sealed with the receiver. When the collecting device is sampled, the receiver holding the sample is removed, and the cap is closed. The inner dripper is in close contact with the receiver to seal the sample.
  • the lid When it is necessary to detect the sample in the receiver, the lid is unscrewed, and the dripper and the lid inside the lid are detached, but are still in close contact with the sample receiver, and the sample can be discharged to the detecting member through the dripper.
  • the "check valve" of the present invention is identical in construction and function to valves known in the art, i.e., ensuring that the fluid passes through the valve from one direction, while the other direction, including the opposite direction, does not circulate, thereby achieving the purpose of controlling the flow direction.
  • a one-way valve ensures that air or sample is discharged outwardly along the receiver, while air or other fluid from the outside cannot be recirculated into the receiver through the one-way valve.
  • Another one-way valve allows fluid such as sample or air to flow into the sample receiver along the one-way valve, but any fluid within the receiver cannot flow back through the one-way valve.
  • the way in which the check valve and the sample receiver are connected is various and can be directly or indirectly connected.
  • the check valve can be connected directly to the sample receiver, or it can be connected via a pipe, connected via a cover placed on the sample receiver, or otherwise connected.
  • Each of the two components can be joined by injection molding, riveting, welding or screwing, or a sealed component can be added at the joint to ensure the tightness of the sample collection device, such as rubber gaskets. Regardless of the manner in which they are connected, the two one-way valves are in communication with the sample collector.
  • the sample receiver has an opening in the upper portion, a cover is mounted on the opening, two ports are opened in the cover, and one of the two ports is connected to a one-way valve, and the two check valves and the receiver cover are One injection is connected together.
  • the sample collection device further includes a sample collection component.
  • the collecting member is in communication with a one-way valve.
  • the two parts are connected and connected in a variety of ways, including but not limited to direct or indirect connection using injection molding, riveting, welding, or spiraling, such as: pipe connection, joint Connect and so on.
  • the sample collection part is made of materials that meet medical standards.
  • the collecting member is made of a soft material that can be bent and freely rotated, and the collecting member made of the material needs to satisfy the function that the collecting member can adjust the angle and direction of the collecting member as needed according to the needs of the operator. For example: Flexible silicone, plastic, plastic and PVC materials that are harmless to the human body.
  • two one-way valves are respectively coupled at both ends of the sample receiver, with the other end of the first one-way valve being coupled to the sample collection member.
  • the receiver compression causes the internal space to become smaller and the pressure to increase.
  • the second one-way valve communicating with the receiver is opened due to the pressure increase.
  • the air in the receiver is discharged outward through the one-way valve to maintain the pressure balance in the receiver; when the external force is removed, the elastic receiver rebounds due to the characteristics of the material itself, then the receptacle returns to the original state, and the inner space becomes larger.
  • the other end of the second one-way valve connected to the sample receiver is connected with a dripper, and after the sample is collected into the receiver, the elastic receiver is squeezed again, because the space compression in the device becomes smaller. The internal pressure is increased, the second check valve connected to the dripper is opened, and the sample in the receiver is discharged along the check valve and the dripper.
  • a sample tube is disposed in the sample receiver, the tube is sealed at one end, one end is open, and the open end is in communication with both the sample receiver and the check valve.
  • the test tube is coupled to the check valve and the sample receiver via a cover located on the receiver.
  • the sample flows into the sample collection device through a one-way valve, it flows directly into the collection tube located in the receptacle.
  • This collection tube can be replaced and used to collect samples in batches.
  • the test tube material can meet medical requirements, and can be a hard material such as a glass product; or an elastic material such as rubber, plastic, silicone or the like. The tube can be used once or repeatedly.
  • an elastic squeezer is provided that is compressible air between the sample receptacle and the second check valve.
  • the elastomeric extruder material can be the same or similar to the elastomeric receiver material and can function the same.
  • the extruder When the extruder is squeezed, the extruder is compressed by the elastic, the internal space is reduced, the pressure is increased, and the increased pressure forces the check valve 2 connected thereto to open, and the air circulates outward in one direction; After the force disappears, such as loosening the extruder, the elastic extrusion The device returns to its original state due to the rebound of the material properties. At this time, due to the increase in the space of the extruder, the gas generates less negative pressure, which forces the second check valve of the exhaust gas to close and forces the connection to the sample collection member and A first one-way valve between the sample receivers is opened such that the sample flows along the first one-way valve into a test tube within the receiver or receiver.
  • the elastomeric squeezer can be replaced with a medical dripper after sampling and the sample is discharged through the dripper.
  • the sample receiver can be made of an inelastic material, such as a licking mouth.
  • connection order of the device may be changed, for example: two check valves are respectively connected to two sides of the elastic extruder, that is, one check valve is only connected to the elastic squeezer; One end of the valve is connected to the elastic extruder, and the other end is connected to the sample receiver and the sample collection part, such as a three-way joint.
  • the flow direction of the two check valves is the same, and the specific implementation method is the same as that of the previous embodiment.
  • the sample collection unit and the sample receiver can be replaced, and the two check valves and the elastic extruder can be reused.
  • the sample collecting member may specifically be a collecting long tube made of a flexible soft material, and a wire may be added to a collecting tube in order to increase its elasticity and strength.
  • a curved corrugation can be designed in the soft plastic collection tube section, which is identical or similar to the straw design currently available on the market for soft packaged beverages.
  • an elastic hollow tip can be provided at the top of the sample collection tube.
  • the one-way valve is not limited to two, and three, four, or more one-way valves may be directly or indirectly connected in the sample collection device.
  • a check valve is connected between the first check valve and the sample receiver, and the opening direction is the same as the first check valve.
  • the receiver may have three openings, each of which is connected to a one-way valve, one check valve to allow liquid or gas samples to enter the sample receiver, and the other two check valves to make the receiver The gas or liquid is discharged; or vice versa, that is, two check valves allow the sample to enter the receiver, and a one-way valve discharges the gas or liquid in the device; this embodiment can increase the collection efficiency of the sample collection device.
  • the present invention also provides a method for collecting a sample, comprising: providing a sample collection device, the device comprising a sample receiver and first and second one-way valves, wherein the three are connected;
  • the first one-way valve is opened, allowing a gas or liquid sample to pass through the one-way valve into the receiver while the second one-way valve is closed.
  • the sample receiver is compressed to form a pressure that causes the second one-way valve to open, causing the first one-way valve to close.
  • a negative pressure is created in the sample receiver; the negative pressure causes the second one-way valve to close and the first one-way valve to open.
  • the sample receiver has elasticity; the elastic receiver is squeezed to compress to form a pressure that causes the second one-way valve to open; the elastic receiver is released, and a negative pressure is formed in the receiver, the negative pressure The two check valves are closed and the first check valve is opened.
  • the apparatus further includes an elastic extruder coupled to the second one-way valve; the elastic extruder is squeezed to compress to form a pressure, and the pressure causes the second one-way valve to open to make the first The check valve is closed; the elastic squeezer is released, a negative pressure is formed in the extruder, the negative pressure closes the second check valve, and the first check valve is opened.
  • an elastic extruder coupled to the second one-way valve; the elastic extruder is squeezed to compress to form a pressure, and the pressure causes the second one-way valve to open to make the first The check valve is closed; the elastic squeezer is released, a negative pressure is formed in the extruder, the negative pressure closes the second check valve, and the first check valve is opened.
  • the invention increases the sealing of the whole collection system by providing two one-way valves, and the vacuum degree is high when the vacuum is squeezed, so that the sample flows along the one-way valve is large, and the flow speed is fast, and a sufficient liquid sample amount can be received. , improved sample collection efficiency. Moreover, the same sample can be collected repeatedly to achieve the required sample size, thereby enabling sufficient testing or testing. In addition, such a collection device prevents the sample from flowing back due to the good sealing effect, thereby eliminating the possibility of sample contamination of the sampler.
  • the invention has simple operation and convenient production, and improves the collection efficiency in the actual operation process.
  • Figure 1 is a cross-sectional view of a first embodiment
  • Figure 2 is a cross-sectional view of a second embodiment
  • Figure 3 is a cross-sectional view of a third embodiment
  • Figure 4 is a cross-sectional view of a fourth embodiment
  • Figure 5 is a cross-sectional view showing a structure of a collecting pipe
  • Figure 6 is a cross-sectional view of the collecting tube shown in Figure 5.
  • Figure 7 is a cross-sectional view of another structure of the collecting tube
  • Sample collection component 100 one end 101 of the collection component; the other end 102 of the collection component; sample receiving One end 401 of the receiver; the other end 402 of the receiver; the first check valve 200; the second check valve 300; the dripper 500; the collection tube 600; the elastic extruder 700; one end of the elastic extruder 701, the other end 702 of the elastic extruder; the collecting tubes 110, 120; the wire 111; the structure of the liquid sample collecting device or the technical terms used are further explained below.
  • the samples referred to in the present invention refer to those substances which can be used to detect, assay or diagnose the presence or absence of an analyte of interest.
  • the sample may be a fluid sample, for example, a liquid sample, which may include blood, plasma, serum, urine, saliva, and various exudates, and may also include a liquid solution formed by pre-treating the solid sample and the semi-solid sample.
  • the sample can also be a gas sample.
  • the collected samples can be used, but are not limited to, immunoassays, chemical tests, enzyme assays, etc. to detect the presence of analytes. Collecting parts
  • the fluid sample collection device includes a fluid collection component.
  • the fluid collection component consists essentially of a water absorbing material that absorbs and stores the liquid sample by chemisorption.
  • the material constituting the fluid collecting member may be selected from the group consisting of polyvinyl alcohol (PVA), foamed plastic, resin, sodium polyacrylate crosslinked product, acrylic acid monovinyl acid copolymer, acrylonitrile polymerized saponified product, vinyl alcohol or sponge, filter paper, glass One or a combination of fibers, nonwovens or acetate.
  • PVA polyvinyl alcohol
  • foamed plastic resin
  • resin sodium polyacrylate crosslinked product
  • acrylic acid monovinyl acid copolymer acrylonitrile polymerized saponified product
  • vinyl alcohol or sponge filter paper
  • filter paper glass One or a combination of fibers, nonwovens or acetate.
  • a more preferred solution is that the material constituting the fluid collecting member is foamed polyvinyl alcohol.
  • the shape of the collecting member may be any suitable shape such as a cylindrical shape, a rectangular parallelepiped shape, a tapered shape, or the like.
  • the collection member 100 includes two ends 101 and 102; one of the ends 101 is for contact with a fluid sample and the fluid sample is absorbed into the collection member. The other end 102 is connected to the one-way valve 200.
  • the collection components are specifically collection tubes 110, 120. detailed description
  • the collection device shown includes a sample collection component 100, a sample receiver 400, a first one-way valve 200, and a second one-way valve 300.
  • the one-way valve 200 is connected to one end 102 of the collecting member and one end of the receiver 401, the one-way valve 300 is connected to the other end 402 of the receiver; the two one-way valves 200, 300 and the sample receiver 400 are connected by a cover on the receiver; the connection between the components of the device is not limited, This embodiment shows a spiral connection.
  • the first check valve 200, the second check valve 300, the collecting member 100 and the receiver 400 are in communication after being connected.
  • the receiver 400 has elasticity and can be made of rubber, plastic, PVC or the like, and can also be made of a transparent material; in this embodiment, it can be specifically a transparent elastic plastic bottle.
  • an external force that is, when the receiver 400 is squeezed, the receiver shrinks the internal space, the air pressure inside the device increases, the pressure forces the second check valve 300 to open, and the air inside the device follows the opened second check valve 300.
  • the external force disappears, that is, after the receiver 400 is released, the receiver 400 returns to the original state due to the elastic characteristic, and the internal space increases to form a negative pressure, and the second check valve 300 is closed due to the negative pressure.
  • the first one-way valve 200 is opened, and the sample in the collecting member 100 flows into the receiver 400 along the first one-way valve 200.
  • the size of the sample receiver can be set according to the needs of sample collection, such as one-time sampling, or large sampling, and the capacity of the receiver can be different.
  • the sample receiver can be any size between 3 ml and 10 ml.
  • FIG 2 is an optimized manner of the first embodiment in which a collection tube 600 is connected to the elastic receiver 400, and the collection tube 600 is in communication with the first one-way valve 200, the second one-way valve 300, and the receiver 400.
  • the receiver when the external force is applied to the elastic receptor 400, the receiver is contracted, and the pressure is increased to cause the check valve 300 to open, and the air in the collection tube 600 and the receiver 400 is discharged. After the external force is removed, the receiver 400 returns to its original state, causing a negative pressure to be generated inside, forcing the check valve 200 to open, and the sample flows into the collection tube 600 along the first check valve 200.
  • the collection tube 600 can be replaced, which is more conducive to repeated collection of samples.
  • the shape of the elastic receiver 400 is not particularly limited as long as it satisfies the function of holding a sample, particularly a sample, such as a cylindrical shape, an elliptical shape or a rectangular shape, etc., as in the case of a normal bottle body.
  • Fig. 3 is another embodiment in which an elastic extruder 700 is provided as compared with the first or second embodiment.
  • the extruder 700 is coupled between the receptacle end 402 and the second check valve 300.
  • the elastic extruder 700 is squeezed, the space inside the extruder is reduced, the pressure is increased, the check valve 300 is forced to open, and the air is discharged; after the extruder is released, the internal space is increased to form a negative pressure due to the negative pressure. Acting, the one-way valve 200 is opened and the sample in the collection member 100 flows into the receiver 400 along the one-way valve 200.
  • Another embodiment may make it easier to replace the sampling components of the collection device, as shown in FIG. 4, the one-way valve 200, which may be located at each end of the elastomeric extruder 700, respectively.
  • the check valve 300 and the end of the extruder Connected to 702 the one-way valve 200 is located between the other end 701 of the extruder and the sample receiver 400.
  • the extruder is squeezed, the space inside the extruder is reduced, the pressure is increased, the check valve 300 is forced to open, and the air is discharged; after the extruder is released, the internal space is increased to form a negative pressure due to the negative pressure.
  • the one-way valve 200 is opened, and the liquid sample in the collecting member 100 flows into the receiver 400 along the collecting member.
  • the collection member and the sample acceptor can be replaced at the same time, and the next sample collection is performed.
  • the device can be reused.
  • the other end of the one-way valve 300 can be connected to the dripper 500, as shown in FIG. 1, after the sample collection is completed, the receiver 400 can be enlarged by squeezing the elastic sample receiver 400. The internal pressure forces the one-way valve 300 to open, and the sample in the receiver flows out through the one-way valve 300 and the dripper 500, and is added to the detecting device or the detecting element.
  • the collecting member may be specific to the collecting tubes 110, 120 as shown in Figs. 5, 6, and 7.
  • the collection tube can be made of a flexible material such as rubber, plastic, plastic, transparent PVC, and the like.
  • a thin wire 111 is added to the hose to increase the elasticity and strength of the collection tube; or, as shown in Figure 7, in the plastic hose section. Corrugated design can be added.

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  • Life Sciences & Earth Sciences (AREA)
  • Hydrology & Water Resources (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention concerne un dispositif de prélèvement d'échantillons liquides comprenant: un réceptacle à échantillons (400) destiné à contenir les échantillons, et deux soupapes unidirectionnelles (200, 300) reliées au réceptacle (400). L'invention concerne également un procédé de prélèvement d'échantillons au moyen du dispositif de prélèvement d'échantillons. Équipé de deux soupapes unidirectionnelles, le dispositif de prélèvement d'échantillons permet une meilleure efficacité de la fermeture étanche et de l'aspiration, et permet de recueillir une quantité appropriée d'échantillons. L'invention permet ainsi une meilleure efficacité du prélèvement d'échantillons, tout en empêchant le reflux d'échantillons pour éviter que l'utilisateur ne soit contaminé par les échantillons.
PCT/CN2011/076897 2010-07-09 2011-07-06 Dispositif de prélèvement d'échantillons liquides Ceased WO2012003797A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN2010102249129A CN102313655A (zh) 2010-07-09 2010-07-09 收集液体样本的装置
CN201010224912.9 2010-07-09

Publications (1)

Publication Number Publication Date
WO2012003797A1 true WO2012003797A1 (fr) 2012-01-12

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WO (1) WO2012003797A1 (fr)

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CN108051260A (zh) * 2014-06-09 2018-05-18 国网山东省电力公司潍坊供电公司 一种操作省力的电力设备用六氟化硫采样设备
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CN108294764A (zh) * 2018-01-30 2018-07-20 刘贞宽 静脉血样采集针及采血组套
CN108403129A (zh) * 2018-03-24 2018-08-17 曹春荣 静脉血样采集针
CN109730696A (zh) * 2018-12-28 2019-05-10 浙江清华柔性电子技术研究院 组织液检测装置
CN110960266B (zh) * 2019-12-23 2022-05-17 大连医科大学附属第二医院 一种可拆卸式小便采集装置
CN113777228A (zh) * 2021-08-16 2021-12-10 北京农业智能装备技术研究中心 植物汁液取样检测装置和方法
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CN201757717U (zh) * 2010-07-09 2011-03-09 艾博生物医药(杭州)有限公司 收集液体样本的装置

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