WO2012089123A1

WO2012089123A1 - Certain dipeptidyl peptidase inhibitors

Info

Publication number: WO2012089123A1
Application number: PCT/CN2011/084824
Authority: WO
Inventors: Weibo Wang; Xingdong ZHAO; Ming Lei; Huajie Zhang; Longcheng Wang; Jian Zhang; Bo FANG; Xia Liu; Zuwen ZHOU
Original assignee: Shanghai Fochon Pharmaceutical Co Ltd
Current assignee: Shanghai Fochon Pharmaceutical Co Ltd
Priority date: 2010-12-29
Filing date: 2011-12-28
Publication date: 2012-07-05
Anticipated expiration: 2013-06-29
Also published as: WO2012088680A1

Abstract

Provided are certain dipeptidyl peptidase inhibitors, pharmaceutical compositions thereof, and methods of use therefor.

Description

CERTAIN DIPEPTIDYL PEPTIDASE INHIBITORS

Field of the Invention

[001] Provided are certain compounds and/or pharmaceutically acceptable salts thereof which can inhibit dipeptidyl peptidase IV (DPP-IV) and may be useful for the treatment of diabetes, such as type II diabetes, as well as hyperglycemia, metabolic syndrome,

hyperinsulinemia, obesity, cardiovascular diseases and disorders such as atherosclerosis, cerebrovascular diseases, diseases and disorders of the central nervous system including schizophrenia, anxiety, bipolar disease, depression, insomnia, cognitive disorders,

gastrointestinal diseases and disorders, cancer, inflammation and inflammatory diseases, respiratory diseases and disorders, musculoskeletal disorders, osteoporosis, menopausal symptoms and disorders, periodontal diseases such as gingivitis, and various immunomodulatory diseases.

Backeround of the Invention

[002] Dipeptidyl peptidase IV (DPP-IV, CD26, EC 3.4.14.5) is a serine protease with specificity for cleaving Xaa-Pro and, to a lesser extent, Xaa-Ala dipeptides from the N-termini of polypeptides and proteins. DPP-IV is a non-classical serine protease in that the catalytic triad of Ser-Asp-His, found in the C-terminal region of the enzyme, is in reverse order to that found in classical serine proteases. DPP-IV is widely expressed in mammalian tissue as a type II integral membrane protein. DPP-IV is expressed on the surface of differentiated epithelial cells of the intestine, liver, kidney proximal tubules, prostate, corpus luteum, and on leukocyte subsets such as lymphocytes and macrophages. A soluble form of the enzyme is found in serum that has structure and function identical to the membrane- bound form of the enzyme but lacks the hydrophobic trans-membrane domain.

[003] DPP-IV has many physiologically relevant substrates such as chemokines, RANTES (regulated on activation normal T-cell expressed and secreted), eotaxin, and macrophage-derived chemokine, neuropeptides such as NPY (neuropeptide Y) and substance P₅ vasoactive peptides, and incretins such as GLP-1 (glucagon-like peptide-1) and GIP (gastric inhibitory peptide/ glucose-dependent insulinotropic polypeptide). [004] GLP-1 (7-36) is a 29 amino-acid peptide derived by post-translational processing of proglucagon in the small intestine. GLP-1 (7-36) may have multiple actions in vivo, for example, the stimulation of insulin secretion, inhibition of glucagon secretion, the promotion of satiety, and the slowing of gastric emptying. Based on its physiological profile, the actions of GLP-1 (7-36) are believed to be beneficial in the treatment of type II diabetes and potentially obesity. For example, exogenous administration of GLP-1 (7-36) (continuous infusion) in diabetic patients has been found to be efficacious in this patient population. Unfortunately, GLP- 1 (7-36) can be degraded rapidly in vivo and has been shown to have a short half-life in vivo (ti/2=1.5 minutes).

[005] Based on a study of genetically bred DPP-IV knockout mice and on in vivo/ in vitro studies with selective DPP-IV inhibitors, DPP-IV has been shown to be the primary degrading enzyme of GLP-1 (7-36) in vivo. GLP-1 (7-36) can be degraded by DPP-IV efficiently to GLP-1 (9-36), which has been speculated to act as a physiological antagonist to GLP-1 (7-36). Inhibiting DPP-IV in vivo is therefore believed to be useful for potentiating endogenous levels of GLP-1 (7-36) and attenuating the formation of its antagonist GLP-1 (9-36). Thus, DPP-IV inhibitors are believed to be useful agents for the treatment of conditions mediated by DPP-IV, such as diabetes and further such as, type II diabetes mellitus, diabetic dislipidemia, conditions of impaired glucose tolerance (IGT), conditions of impaired fasting plasma glucose (IFG), metabolic acidosis, ketosis, appetite regulation and obesity.

[006] The inhibition of DPP-IV can provide for an attractive therapeutic treatment for type II diabetes and obesity. Although DPP-IV inhibitors may have demonstrated improved glucose tolerance in type II diabetes, many suffer from having short half-life and toxicity.

Therefore, there is a need for new DPP-IV inhibitors that have at least one advantageous property selected from potency, stability, selectivity, toxicity and pharmacodynamics properties as an alternative for the treatment of type II diabetes. In this regard, a novel class of DPP-IV inhibitors is provided herein.

Summary of the Invention

[007] Provided is at least one compound of formula (I):

and/or at least one pharmaceutically acceptable salt thereof, wherein

Ar is aryl unsubstituted or substituted with one to five R¹ substituents;

each R¹ is independently selected from:

halogen,

cyano,

hydroxy,

Ci_6 alkyl, wherein alkyl is unsubstituted or substituted with one to five halogens, and Ci_6 alkoxy, wherein alkoxy is unsubstituted or substituted with one to five halogens;

2 3 4

X and Y are each independently selected from CR R , and NR ;

2 3

IT and R are each independently selected from

hydrogen,

halogen,

hydroxy,

R⁴ is independently selected from

hydrogen,

Ci_6 alkyl, wherein alkyl is unsubstituted or substituted with one to five substituents independently selected from halogen and hydroxy,

(CH₂)_n-aryl, wherein aryl is unsubstituted or substituted with one to five substituents independently selected from hydroxy, halogen, cyano, C0₂H, Ci_₆ alkyloxycarbonyl, Ci_6 alkyl, and Ci_₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens,

(CH₂)_n-heteroaryl, wherein heteroaryl is unsubstituted or substituted with one to three substituents independently selected from hydroxy, halogen, cyano, C0₂H, Ci_₆ alkyloxycarbonyl, Ci_₆ alkyl, and Ci_₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens,

(CH₂)_n-heterocyclyl, wherein heterocyclyl is unsubstituted or substituted with one to three substituents independently selected from oxo, hydroxy, halogen, cyano, C0₂H, Ci_6 alkyloxycarbonyl, Ci_₆ alkyl, and Ci_₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens,

(CH₂)_n-C3_6 cycloalkyl, wherein cycloalkyl is unsubstituted or substituted with one to three substituents independently selected from halogen, hydroxy, cyano, C0₂H, Ci_₆ alkyloxycarbonyl, Ci_₆ alkyl, and Ci_₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens,

(CH₂)_n- -COCi_6 alkyl,

(CH₂)_n- -COCi_6 cycloalkyl,

(CH₂)_n- -C0₂Ci_6 alkyl,

(CH₂)_n- -C0₂Ci_6 cycloalkyl,

(CH₂)_n- -CONR⁵R⁶,

(CH₂)_n- -S(0)_mR⁶, and

(CH₂)_n- -S0₂NR⁵R⁶,

wherein any individual methylene (CH₂) carbon atom in (CH₂)_n is unsubstituted or substituted with one to two groups independently selected from halogen, hydroxy, Ci_6 alkyl, and Ci_₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens;

R⁶ are each independently selected from

hydrogen,

(CH₂)_n-aryl,

(CH₂)_n-C₃_6 cycloalkyl, and

Ci_6 alkyl,

wherein alkyl is unsubstituted or substituted with one to five substituents independently selected from halogen and hydroxy, and

further wherein aryl and cycloalkyl are each independently unsubstituted or substituted with one to five substituents independently selected from halogen, hydroxy, Ci_₆ alkyl, and Ci_₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens; or R⁵ and R⁶ together with the nitrogen atom to which they are attached form a heterocyclic ring selected from azetidine, pyrrolidine, piperidine, piperazine, and morpholine wherein the heterocyclic ring is unsubstituted or substituted with one to three substituents independently selected from halogen, hydroxy, Ci_₆ alkyl, and Ci_₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens; m is selected from 0, 1 and 2; and

n is selected from 0, 1, 2, and 3.

[008] Provided is a pharmaceutical composition, which comprises at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein, and at least one pharmaceutically acceptable carrier.

[009] Provided is a method for treating a condition selected from insulin resistance, hyperglycemia, and Type II diabetes comprising administering to a patient in recognized need thereof an effective amount of at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein.

Detailed Description of the Invention

[010] As used herein the following definitions are applicable.

[011] The term "alkyl" refers to both branched and straight-chain saturated aliphatic hydrocarbon groups having the specified number of carbon atoms. Unless otherwise specified, "alkyl" refers to Q-C₆ alkyl. For example, Ci-C₆, as in "Q_6 alkyl" is defined to include groups having 1, 2, 3, 4, 5, and 6 carbons in a linear or branched arrangement. For example, "Q_6 alkyl" includes but is not limited to methyl, ethyl, n-propyl, i-propyl, n-butyl, t-butyl, i-butyl, pentyl, and hexyl.

[012] The term "cycloalkyl" means a saturated aliphatic cyclic hydrocarbon group having the specified number of carbon atoms. Unless otherwise specified, "cycloalkyl" refers to C₃_6 cycloalkyl. For example, "cycloalkyl" includes but is not limited to cyclopropyl, methyl- cyclopropyl, 2,2-dimethyl-cyclobutyl, 2-ethyl-cyclopentyl, and cyclohexyl. [013] The term "alkoxy" refers to either a cyclic or non-cyclic alkyl group of indicated number of carbon atoms attached through an oxygen bridge. "Alkoxy" therefore encompasses the definitions of alkyl and cycloalkyl above.

[014] The term "aryl" encompasses:

5- and 6-membered carbocyclic aromatic rings, for example, benzene;

bicyclic ring systems wherein at least one ring is carbocyclic and aromatic, for example, naphthalene, indane, and 1,2,3,4-tetrahydroquinoline; and

tricyclic ring systems wherein at least one ring is carbocyclic and aromatic, for example, fiuorene. In cases where the aryl substituent is bicyclic or tricyclic and at least one ring is non-aromatic, it is understood that attachment is via the aromatic ring.

[015] For example, aryl includes 5- and 6-membered carbocyclic aromatic rings fused to a 5- to 7-membered heterocyclic ring containing one or more heteroatoms selected from N, O, and S, provided that the point of attachment is at the carbocyclic aromatic ring. Bivalent radicals formed from substituted benzene derivatives and having the free valences at ring atoms are named as substituted phenylene radicals. Bivalent radicals derived from univalent polycyclic hydrocarbon radicals whose names end in "-yl" by removal of one hydrogen atom from the carbon atom with the free valence are named by adding "-idene" to the name of the

corresponding univalent radical, e.g., a naphthyl group with two points of attachment is termed naphthylidene. Aryl, however, does not encompass or overlap in any way with heteroaryl, separately defined below. Hence, if one or more carbocyclic aromatic rings are fused with a heterocyclic aromatic ring, the resulting ring system is heteroaryl, not aryl, as defined herein.

[016] The term "halogen" (or "halo") refers to fluorine, chlorine, bromine and iodine.

[017] The term "heteroaryl" refers to

5- to 8-membered aromatic, monocyclic rings containing one or more, for example, from

1 to 4, or, in some embodiments, from 1 to 3, heteroatoms selected from N, O, and S, with the remaining ring atoms being carbon;

8- to 12-membered bicyclic rings containing one or more, for example, from 1 to 4, or, in some embodiments, from 1 to 3, heteroatoms selected from , O, and S, with the remaining ring atoms being carbon and wherein at least one heteroatom is present in an aromatic ring; and 11- to 14-membered tricyclic rings containing one or more, for example, from 1 to 4, or in some embodiments, from 1 to 3, heteroatoms selected from N, O, and S, with the remaining ring atoms being carbon and wherein at least one heteroatom is present in an aromatic ring.

[018] When the total number of S and O atoms in the heteroaryl group exceeds 1 , those heteroatoms are not adjacent to one another. In some embodiments, the total number of S and O atoms in the heteroaryl group is not more than 2. In some embodiments, the total number of S and O atoms in the aromatic heterocycle is not more than 1.

[019] Examples of heteroaryl groups include, but are not limited to, (as numbered from the linkage position assigned priority 1), 2-pyridyl, 3-pyridyl, 4-pyridyl, 2,3-pyrazinyl, 3,4- pyrazinyl, 2,4-pyrimidinyl, 3,5-pyrimidinyl, 1-pyrazolyl, 2,3-pyrazolyl, 2,4-imidazolinyl, isoxazolyl, oxazolyl, thiazolyl, thiadiazolyl, tetrazolyl, thienyl, benzothienyl, furyl, benzofuryl, benzoimidazolinyl, indolinyl, pyridizinyl, triazolyl, quinolinyl, pyrazolyl, and 5,6,7,8- tetrahydroisoquinoline.

[020] Further heteroaryl groups include but are not limited to pyrrolyl, isothiazolyl, triazinyl, pyrazinyl, pyridazinyl, indolyl, benzotriazolyl, quinoxalinyl, and isoquinolinyl,. As with the definition of heterocycle below, "heteroaryl" is also understood to include the N-oxide derivative of any nitrogen-containing heteroaryl.

[021] Bivalent radicals derived from univalent heteroaryl radicals whose names end in "-yl" by removal of one hydrogen atom from the atom with the free valence are named by adding "-idene" to the name of the corresponding univalent radical, e.g., a pyridyl group with two points of attachment is a pyridylidene. Heteroaryl does not encompass or overlap with aryl as defined above.

[022] In cases where the heteroaryl substituent is bicyclic or tricyclic and at least one ring is non-aromatic or contains no heteroatoms, it is understood that attachment is via the aromatic ring or via the heteroatom containing ring, respectively.

[023] The term "heterocycle" (and variations thereof such as "heterocyclic", or "heterocyclyl") broadly refers to a single aliphatic ring, usually with 3 to 7 ring atoms, containing at least 2 carbon atoms in addition to 1-3 heteroatoms independently selected from oxygen, sulfur, and nitrogen, as well as combinations comprising at least one of the foregoing heteroatoms. "Heterocycle" also refers to 5- to 7-membered heterocyclic ring containing one or more heteroatoms selected from N, O, and S fused with 5- and 6-membered carbocyclic aromatic ring, provided that the point of attachment is at the heterocyclic ring. The rings may be saturated or have one or more double bonds (i.e. partially unsaturated). The heterocycle can be substituted by oxo. The point of the attachment may be carbon or heteroatom in the heterocyclic ring, provided that attachment results in the creation of a stable structure. When the heterocyclic ring has substituents, it is understood that the substituents may be attached to any atom in the ring, whether a heteroatom or a carbon atom, provided that a stable chemical structure results.

Heterocycle does not overlap with heteroaryl.

[024] Suitable heterocycles include, for example (as numbered from the linkage position assigned priority 1), 1-pyrrolidinyl, 2-pyrrolidinyl, 2,4-imidazolidinyl, 2,3-pyrazolidinyl, 1-piperidinyl, 2-piperidinyl, 3-piperidinyl, 4-piperidinyl, and 2,5-piperazinyl. Morpholinyl groups are also contemplated, including 2-morpholinyl and 3-morpholinyl (numbered wherein the oxygen is assigned priority 1). Substituted heterocycle also includes ring systems substituted with one or more oxo moieties, such as piped dinyl N-oxide, morpholinyl-N-oxide, 1-oxo-l- thiomorpholinyl and 1,1-dioxo-l-thiomorpholinyl.

[025] For avoidance of doubt, reference, for example, to substitution of alkyl, cycloalkyl, heterocyclyl, aryl, and/or heteroaryl refers to substitution of each of those groups individually as well as to substitutions of combinations of those groups.

[026] The term "pharmaceutically acceptable salts" refers to salts prepared from pharmaceutically acceptable non-toxic bases or acids including inorganic or organic bases and inorganic or organic acids. Salts derived from inorganic bases may be selected, for example, from aluminum, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic, manganous, potassium, sodium, and zinc salts. Further, for example, the pharmaceutically acceptable salts derived from inorganic bases may be selected from ammonium, calcium, magnesium, potassium, and sodium salts. Salts in the solid form may exist in one or more crystal structures, and may also be in the form of hydrates. Salts derived from pharmaceutically acceptable organic non-toxic bases may be selected, for example, from salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines, and basic ion exchange resins, such as arginine, betaine, caffeine, choline, Ν,Ν'-dibenzylethylene-diamine, diethylamine, 2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethyl-morpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine, and tripropylamine, tromethamine.

[027] When the compound disclosed herein is basic, salts may be prepared using at least one pharmaceutically acceptable non-toxic acid, selected from inorganic and organic acids. Such acid may be selected, for example, from acetic, benzenesulfonic, benzoic, camphorsulfonic, citric, ethanesulfonic, fumaric, gluconic, glutamic, hydrobromic, hydrochloric, isethionic, lactic, maleic, malic, mandelic, methanesulfonic, mucic, nitric, pamoic, pantothenic, phosphoric, succinic, sulfuric, tartaric, and p-toluenesulfonic acids. In some embodiments, such acid may be selected, for example, from citric, hydrobromic, hydrochloric, maleic, phosphoric, sulfuric, fumaric, and tartaric acids.

[028] The term "protecting group" or "Pg" refers to a substituent that can be commonly employed to block or protect a certain functionality while reacting other functional groups on the compound. For example, an "amino-protecting group" is a substituent attached to an amino group that blocks or protects the amino functionality in the compound. Suitable amino-protecting groups include but are not limited to acetyl, trifluoroacetyl, t-butoxycarbonyl (BOC), benzyloxycarbonyl (CBZ) and 9-fluorenylmethylenoxycarbonyl (Fmoc). Similarly, a "hydroxy- protecting group" refers to a substituent of a hydroxy group that blocks or protects the hydroxy functionality. Suitable protecting groups include but are not limited to acetyl and silyl. A

"carboxy-protecting group" refers to a substituent of the carboxy group that blocks or protects the carboxy functionality. Common carboxy-protecting groups include --CH₂CH₂SO₂PI1, cyanoethyl, 2-(trimethylsilyl)ethyl, 2-(trimethylsilyl)ethoxymethyl, 2-(p-toluenesulfonyl)ethyl, 2-(p-nitrophenylsulfenyl)ethyl, 2-(diphenylphosphino)-ethyl, nitroethyl and the like. For a general description of protecting groups and their use, see T. W. Greene, Protective Groups in Organic Synthesis, John Wiley & Sons, New York, 1991.

[029] The terms "administration of and or "administering" at least one compound and/or at least one pharmaceutically acceptable salt should be understood to mean providing at least one compound and/or at least one pharmaceutically acceptable salt thereof to the individual in recognized need of treatment.

[030] The term "effective amount" means the amount of the at least one compound and/or at least one pharmaceutically acceptable salt that will elicit the biological or medical response of a tissue, system, animal or human that is being sought by the researcher, veterinarian, medical doctor or other clinician.

[031] The term "composition" as used herein is intended to encompass a product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combination of the specified ingredients in the specified amounts. Such term in relation to pharmaceutical composition, is intended to encompass a product comprising the active ingredient (s), and the inert ingredient (s) that make up the carrier, as well as any product which results, directly or indirectly, from combination, complexation or aggregation of any two or more of the ingredients, or from dissociation of one or more of the ingredients, or from other types of reactions or interactions of one or more of the ingredients.

[032] By "pharmaceutically acceptable" it is meant compatible with the other ingredients of the formulation and not unacceptably deleterious to the recipient thereof.

[033] Provided is at least one compound of formula (I):

and/or at least one pharmaceutically acceptable salt thereof, wherein

Ar is aryl unsubstituted or substituted with one to five R substituents;

each R¹ is independently selected from:

halogen,

cyano,

hydroxy,

X and Y are each independently selected from CR²R³, and NR⁴;

R² and R³ are each independently selected from

hydrogen,

halogen,

hydroxy,

R⁴ is independently selected from

hydrogen,

(CH₂)_n- -COCi_6 alkyl,

(CH₂)_n- -COCi_6 cycloalkyl,

(CH₂)_n- -C0₂d_₆ alkyl,

(CH₂)_n- -C0₂Ci_6 cycloalkyl,

(CH₂)_n- -CONR⁵R⁶,

(CH₂)_n- -S(0)_mR⁶, and

(CH₂)_n- -S0₂NR⁵R⁶,

R⁵ and R⁶ are each independently selected from:

hydrogen,

(CH₂)_n-aryl,

(CH₂)_n-C3_6 cycloalkyl, and

Ci_6 alkyl,

wherein alkyl is unsubstituted or substituted with one to five substituents independently selected from halogen and hydroxy,and

further wherein aryl and cycloalkyl are each independently unsubstituted or substituted with one to five substituents independently selected from halogen, hydroxy, Ci_₆ alkyl, and Ci_₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens; or R⁵ and R⁶ together with the nitrogen atom to which they are attached form a heterocyclic ring selected from azetidine, pyrrolidine, piperidine, piperazine, and morpholine wherein the heterocyclic ring is unsubstituted or substituted with one to three substituents independently selected from halogen, hydroxy, Ci_₆ alkyl, and Ci_₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens;

m is selected from 0, 1 and 2; and

n is selected from 0, 1, 2, and 3.

[034] In some embodiments Ar is phenyl substituted with one to five R¹ substituents.

[035] In some embodiments, R¹ is halogen.

[036] In some embodiments, Ar is selected from 2,4,5-trifluorophenyl and 2,5- difluorophenyl. In some embodiments, Ar is 2,4,5-trifluorophenyl. In some embodiments, Ar is 2,5-difiuorophenyl.

[037] In some embodiments, X is CR²R³ and Y is CR²R³. In some embodiments, X is NR⁴ and Y is CR²R³. In some embodiments, X is CR²R³ and Y is NR⁴.

[038] In some embodiments, R² and R³ are hydrogen.

[039] In some embodiments, R⁴ is selected from hydrogen, Ci_₆ alkyl , (CH₂)_n-COCi_6 alkyl, (CH₂)_n-C0₂Ci_6 alkyl, (CH2)_n-CONR⁵R⁶, (CH₂)_n-S(0)_mR⁶, and (CH₂)_n-S0₂NR⁵R⁶, wherein n is 0 and m is 2. [040] In some embodiments, R⁴ is selected from H, CH₃, COCH₃, C0₂CH₃, CONH₂, S0₂CH₃, and S0₂NH₂.

[041] In some embodiments, X is NR⁴ and Y is CR²R³, Ar is 2,4,5-trifluorophenyl, wherein R² and R³ are hydrogen, and R⁴ is selected from H, C¾, COCH₃, C0₂CH₃, CONH₂, S0₂C¾, and S0₂NH₂.

[042] Also provided is least one compound and/or at least one pharmaceutically acceptable salt thereof, selected from:

(7, ,Sii)-8-(2,4,5-trifiuoiOphenyl)-l,2,3,4,6,7,8,9-octahydrobenzo[4,5]imidazo[l,2- a]pyrazin-7-amine;

(7ii,85)-7-(2,4,5-trifiuorophenyl)-l,2,3,4,6,7,8,9-octahydrobenzo[4,5]imidazo[l,2- a]pyridin-8 -amine;

(75, 8R)-methyl 7-amino-8-(2,4,5-trifluorophenyl)-3,4,6,7,8,9- hexahydrobenzo[4,5]imidazo[l ,2-a]pyrazine-2(lH)-carboxylate;

(75,8R)-methyl 7-amino-8-(2,5-difiuorophenyl)-3,4,6,7,8,9- hexahydrobenzo[4,5]imidazo[l,2-a]pyrazine-2(lH)-carboxylate;

(75, 8R)-2-methyl-8-(2,4,5-trifluorophenyl)-l,2,3,4,6,7,8,9- octahydrobenzo[4,5]imidazo[l ,2-a]pyrazin-7-amine;

l-((75,Sii)-7-amino-8-(2,4,5-trifiuorophenyl)-3,4,6,7,8,9- hexahydrobenzo[4,5]imidazo[ l,2-a]pyrazin-2(lH)-yl)ethanone;

(75, 8R)-2-(methylsulfonyl)-8-(2,4,5-trifluorophenyl)-l , 2,3,4,6,7,8,9- octahydrobenzo[4,5]imidazo[l ,2-a]pyrazin-7-amine;

(75,Sii)-7-amino-8-(2,4,5-trifluorophenyl)-3,4,6,7,8,9-hexahydrobenzo[4,5]imidazo[l ,2- a]pyrazine-2(lH)-sulfonamide;

(75,Sii)-7-amino-8-(2,4,5-trifluorophenyl)-3,4,6,7,8,9-hexahydrobenzo[4,5]imidazo[l,2- a]pyrazine-2(lH)-carboxamide;

l-((75,S i)-7-amino-8-(2,4,5-trifluorophenyl)-3,4,6,7,8,9- hexahydrobenzo[4,5]imidazo[l ,2-a]pyrimidin-l(2H)-yl)ethanone;(75,Sii)-l-methyl-8- (2,4,5-trifluorophenyl)-l , 2,3,4,6, 7,8, 9-octahydrobenzo[4,5]imidazo[l,2-a]pyrimidin-7- amine;

(75,8R)-methyl 7-amino-8-(2,4,5-trifiuorophenyl)-3,4,6,7,8,9- hexahydrobenzo[4,5]imidazo[ 1 ,2-a]pyrimidine- 1 (2H)-carboxylate; and (7,S',Sii)-8-(2,4,5-trifluorophenyl)-l,2,3,4,6,7,8,9-octahydrobenzo[4,5]imidazo[l,2- a]pyrimidin-7-amine.

[043] Also provided is a method of treating a condition responsive to inhibition of dipeptidyl peptidase-IV enzyme comprising administering to a patient in recognized need thereof an effective amount of the at least one compound and/or at least one pharmaceutically acceptable salt described herein.

[044] Also provided is a method of treating a condition selected from insulin resistance, hyperglycemia, and Type II diabetes comprising administering to a patient in recognized need thereof an effective amount of the at least one compound and/or at least one pharmaceutically acceptable salt described herein.

[045] In some embodiments, the at least one compound and/or at least one

pharmaceutically acceptable salt thereof described herein can be useful in a method of inhibiting the dipeptidyl peptidase-IV enzyme in a patient such as a mammal in recognized need of such inhibition comprising the administration of an effective amount of the at least one compound and/or at least one pharmaceutically acceptable salt described herein.. Also provided is the use of the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein as inhibitors of dipeptidyl peptidase-IV enzyme activity.

[046] In addition to primates, such as humans, a variety of other mammals can be treated according to the methods described herein. For instance, mammals including, but not limited to, cows, sheep, goats, horses, dogs, cats, guinea pigs, rats or other bovine, ovine, equine, canine, feline, rodent or murine species can be treated. However, the method can also be practiced in other species, such as avian species (e. g., chickens).

[047] Also provided is a composition comprising the at least one compound and/or at least one pharmaceutically acceptable salt described herein and at least one pharmaceutically acceptable carrier.

[048] Also provided is a method for the manufacture of a medicament for inhibiting dipeptidyl peptidase-IV enzyme activity in humans and animals comprising combining at least one compound and/or at least one pharmaceutically acceptable salt described herein with at least one pharmaceutically acceptable carrier.

[049] In some embodiments, the patient is a mammal, such as a human being, male or female, in whom inhibition of dipeptidyl peptidase-IV enzyme activity is desired. [050] Accordingly, the pharmaceutical compositions described herein encompass any composition made by admixing at least one compound of formula (I) and/or at least one pharmaceutically acceptable salt thereof and at least one pharmaceutically acceptable carrier.

[051] Dipeptidyl peptidase-IV enzyme (DPP-IV) is a cell surface protein that has been implicated in a wide range of biological functions. It has a broad tissue distribution (intestine, kidney, liver, pancreas, placenta, thymus, spleen, epithelial cells, vascular endothelium, lymphoid and myeloid cells, serum), and distinct tissue and cell-type expression levels. DPP-IV is identical to the T cell activation marker CD26, and it can cleave a number of

immunoregulatory, endocrine, and neurological peptides in vitro. This has suggested a potential role for this peptidase in a variety of disease processes in humans or other species.

[052] Accordingly, the compounds and/or pharmaceutically acceptable salts described herein can be useful in a method for the treatment of the following diseases, disorders and conditions.

[053] Type II Diabetes and Related Disorders: It is well established that the incretins GLP- 1 and GIP are rapidly inactivated in vivo by DPP-IV. Studies with DPP-IV ^{( / _)}-deficient mice and preliminary clinical trials indicate that DPP-IV inhibition increases the steady state concentrations of GLP-1 and GIP, resulting in improved glucose tolerance. By analogy to GLP-1 and GIP, it is likely that other glucagon family peptides involved in glucose regulation are also inactivated by DPP-IV (eg. PACAP). Inactivation of these peptides by DPP-IV may also play a role in glucose homeostasis.

[054] DPP-IV inhibitors described hereintherefore may have utility in the treatment of type II diabetes and in the treatment of the numerous conditions that often accompany Type II diabetes, including but being not limited to Syndrome X (also known as Metabolic Syndrome), reactive hypoglycemia, and diabetic dyslipidemia. Obesity, discussed below, is another condition that can be often found with Type II diabetes that may respond to treatment with the DPP-IV inhibitors described herein.

[055] The compounds and/or pharmaceutically acceptable salts described herein may have utility in treating one or more of the following conditions or diseases: (1) hyperglycemia, (2) low glucose tolerance, (3) insulin resistance, (4) obesity, (5) lipid disorders, (6) dyslipidemia, (7) hyperlipidemia, (8) hypertriglyceridemia, (9) hypercholesterolemia, (10) low HDL levels, (1 1) high LDL levels, (12) atherosclerosis and its sequelae, (13) vascular restenosis, (14) irritable bowel syndrome, (15) inflammatory bowel disease, including Crohn's disease and ulcerative colitis, (16) other inflammatory conditions, (17) pancreatitis, (18) abdominal obesity, (19) neurodegenerative disease, (20) retinopathy, (21) nephropathy, (22) neuropathy, (23) Syndrome X, (24) ovarian hyperandrogenism (polycystic ovarian syndrome), (25) Type II diabetes, (26) growth hormone deficiency, (27) neutropenia, (28) neuronal disorders, (29) tumor metastasis, (30) benign prostatic hypertrophy, (32) gingivitis, (33) hypertension, (34) osteoporosis, and other conditions that may be treated by inhibition of DPP-IV.

[056] Obesity: DPP-IV inhibitors described herein may be useful for the treatment of obesity. This is based on the observed inhibitory effects on food intake and gastric emptying of GLP-1 and GLP-2.

[057] Exogenous administration of GLP-1 in humans significantly decreases food intake and slows gastric emptying (Am. J. Physio., 277: R910-R916 (1999)). ICV administration of GLP-1 in rats and mice also has profound effects on food intake (Nature Medicine, 2: 1254- 1258 (1996)).

[058] This inhibition of feeding is not observed in GLP-IR^ mice, indicating that these effects may be mediated through brain GLP-1 receptors. By analogy to GLP-1, it is likely that GLP-2 can be also regulated by DPP-IV. ICV administration of GLP-2 may also inhibit food intake, analogous to the effects observed with GLP-1 (Nature Medicine, 6: 802-807 (2000)). In addition, studies with DPP-IV deficient mice may suggest that these animals are resistant to diet- induced obesity and associated pathology (e. g. hyperinsulinonemia).

[059] Growth Hormone Deficiency: DPP-IV inhibition may be useful for the treatment of growth hormone deficiency, based on the hypothesis that growth-hormone releasing factor (GRF), a peptide that stimulates release of growth hormone from the anterior pituitary, is cleaved by the DPP-IV enzyme in vivo (WO 00/56297). The following data may provide evidence that GRF can be an endogenous substrate: (1) GRF is efficiently cleaved in vitro to generate the inactive product GRF [3-44] (BBA 1122: 147-153 (1992) ) ; (2) GRF is rapidly degraded in plasma to GRF [3-44]; this is prevented by the DPP-IV inhibitor diprotin A; and (3) GRF [3-44] is found in the plasma of a human GRF transgenic pig (J. Clin. Invest., 83: 1533-1540 (1989)). Thus DPP-IV inhibitors may be useful for the same spectrum of indications which have been considered for growth hormone secretagogues. [060] Intestinal Injury: The potential for using DPP-IV inhibitors for the treatment of intestinal injury can be suggested by the results of studies indicating that glucagon-like peptide-2 (GLP-2), a likely endogenous substrate for DPP-IV, may exhibit trophic effects on the intestinal epithelium (Regulatory Peptides. 90: 27-32 (2000) ). Administration of GLP-2 results in increased small bowel mass in rodents and attenuates intestinal injury in rodent models of colitis and enteritis.

[061] Immunosuppression: DPP-IV inhibition may be useful for modulation of the immune response, based upon studies implicating the DPP-IV enzyme in T cell activation and in chemokine processing, and efficacy of DPP-IV inhibitors in in vivo models of disease. DPP-IV has been shown to be identical to CD26, a cell surface marker for activated immune cells. The expression of CD26 can be regulated by the differentiation and activation status of immune cells. It is for example accepted that CD26 functions as a co-stimulatory molecule in in vitro models of T cell activation. A number of chemokines contain proline in the penultimate position, presumably to protect them from degradation by non-specific aminopeptidases. Many of these have been shown to be processed in vitro by DPP-IV. In several cases (RANTES, LD78-beta, MDC, eotaxin, SDF-1 alpha), cleavage can result in an altered activity in chemotaxis and signaling assays.

[062] Receptor selectivity also appears to be modified in some cases (RANTES).

Multiple N- terminally truncated forms of a number of chemokines have been identified in in vitro cell culture systems, including the predicted products of DPP-IV hydrolysis.

[063] DPP-IV inhibitors have been shown to be efficacious immunosuppressants in animal models of transplantation and arthritis. Prodipine (Pro-Pro-diphenyl-phosphonate), an irreversible inhibitor of DPP-IV, was shown to double cardiac allograft survival in rats from day 7 to day 14 (Transplantation, 63: 1495-1500 (1997)). DPP-IV inhibitors have been tested in collagen and alkyldiamine-induced arthritis in rats and showed a statistically significant attenuation of hind paw swelling in this model [Int. J. Immunopharmacology, 19: 15-24 (1997) and Immunopharmacology, 40: 21-26 (1998)]. DPP-IV is upregulated in a number of autoimmune diseases including rheumatoid arthritis, multiple sclerosis, Graves' disease, and Hashimoto's thyroiditis (Immunology Today, 20: 367-375 (1999)).

[064] HIV Infection: DPP-IV inhibition may be useful for the treatment of EGV infection or AIDS because a number of chemokines which inhibit HIV cell entry are potential OC substrates for DPP-IV (Immunology Today 20: 367-375 (1999)). In the case of SDF-1 alpha, cleavage decreases antiviral activity (PNAS, 95: 6331-6 (1998)). Thus, stabilization of SDF-1 alpha through inhibition of DPP-IV would be expected to decrease HTV infectivity.

[065] Hematopoiesis: DPP-IV inhibition may be useful for the treatment of

hematopiesis because DPP-IV may be involved in hematopoiesis. A DPP-IV inhibitor, Val- Boro-Pro, stimulated hematopoiesis in a mouse model of cyclophosphamide-induced neutropenia (WO 99/56753).

[066] Neuronal Disorders: DPP-IV inhibition may be useful for the treatment of various neuronal or psychiatric disorders because a number of peptides implicated in a variety of neuronal processes are cleaved in vitro by DPP-IV. A DPP-IV inhibitor thus may have a therapeutic benefit in the treatment of neuronal disorders. Endomorphin-2, beta-casomorphin, and substance P have all been shown to be in vitro substrates for DPP-IV. In an electric shock jump test model of analgesia in rats, a DPP-IV inhibitor showed a significant effect that was independent of the presence of exogenous endomorphin-2 (Brain Research, 815: 278-286

(1999)). Neuroprotective and neuroregenerative effects of DPP-IV inhibitors were also evidenced by the inhibitors' ability to protect motor neurons from excitotoxic cell death, to protect striatal innervation of dopaminergic neurons when administered concurrently with MPTP, and to promote recovery of striatal innervation density when given in a therapeutic manner following MPTP treatment [see Yong-Q. Wu, et al., "Neuroprotective Effects of Inhibitors of Dipeptidyl Peptidase-IV in vitro and in vivo, "Int. Conf. On Dipeptidyl Aminopeptidases: Basic Science and Clinical Applications, September 26-29, 2002 (Berlin, Germany)].

[067] Anxiety rats naturally deficient in DPP-IV have an anxiolytic phenotype (WO 02/34243; Karl et al., Physiol. Behav. 2003). DPP-IV deficient mice also have an anxiolytic phenotype using the Porsolt and light/dark models. Thus DPP-IV inhibitors described herein may prove useful for treating anxiety and related disorders.

[068] Memory and Cognition GLP-1 agonists can be active in models of learning (passive avoidance, Morris water maze) and neuronal injury (kainate-induced neuronal apoptosis) as demonstrated by During et al. (Nature Med. 9: 1173-1 179 (2003) ). The results may suggest a physiological role for GLP-1 in learning and neuroprotection. Stabilization of GLP-1 by DPP-IV inhibitors are expected to show similar effects. [069] Tumor Invasion and Metastasis: DPP-IV inhibition may be useful for the treatment of tumor invasion and metastasis because an increase or decrease in expression of several ectopeptidases including DPP-IV has been observed during the transformation of normal cells to a malignant phenotype (J. Exp. Med., 190: 301-305 (1999)). Up-or down-regulation of these proteins appears to be tissue and cell-type specific. For example, increased CD26/DPP-IV expression has been observed on T cell lymphoma, T cell acute lymphoblastic leukemia, cell- derived thyroid carcinomas, basal cell carcinomas, and breast carcinomas. Thus, DPP-IV inhibitors may have utility in the treatment of such carcinomas.

[070] Benign Prostatic Hypertrophy: DPP-IV inhibition may be useful for the treatment of benign prostatic hypertrophy because increased DPP-IV activity was noted in prostate tissue from patients with BPH (Eur. J. Clin. Chem. Clin. Biochem., 30: 333-338 (1992)).

[071] Sperm motility/male contraception: DPP-IV inhibition may be useful for the altering sperm motility and for male contraception because in seminal fluid, prostatosomes, which are prostate derived organelles important for sperm motility, possess very high levels of DPP-IV activity (Eur. J. Clin. Chem. Clin. Biochem., 30: 333-338 (1992)).

[072] Gingivitis: DPP-IV inhibition may be useful for the treatment of gingivitis because DPP-IV activity was found in gingival crevicular fluid and in some studies correlated with periodontal disease severity (Arch. Oral Biol., 37: 167-173 (1992)).

[073] Osteoporosis: DPP-IV inhibition may be useful for the treatment of osteoporosis because GIP receptors are present in osteoblasts.

[074] The compounds and/or pharmaceutically acceptable salts thereof described herein may be further useful in a method for the treatment of the aforementioned diseases, disorders and conditions in combination with other agents.

[075] The compounds and/or pharmaceutically acceptable salts thereof described herein may be used in combination with one or more other drugs in the treatment of diseases or conditions for which compounds of Formula I and/or pharmaceutically acceptable salts thereof or the other drugs may have utility, particularly where the combination of the drugs together are safer or more effective than either drug alone. Such other drug (s) may be administered, by a route and in an amount commonly used therefore, contemporaneously or sequentially with at least one compound of Formula I and/or at least one pharmaceutically acceptable salt thereof. When at least one compound of Formula I and/or at least one pharmaceutically acceptable salt thereof is used contemporaneously with one or more other drugs, a pharmaceutical composition in unit dosage form containing such at least one drug and the at least one compound of Formula I and/or at least one pharmaceutically acceptable salt thereof may be desired. However, the combination therapy may also include therapies in which the at least one compound of Formula I and/or at least one pharmaceutically acceptable salt thereof and one or more other drugs are administered on different overlapping schedules. It is also contemplated that when used in combination with one or more other active ingredients, the compound(s) described herein and the other active ingredients may be used in lower doses than when each is used singly. Accordingly, the pharmaceutical compositions described herein include but are not limited to those that contain one or more other active ingredients, in addition to at least one compound of Formula I and/or at least one pharmaceutically acceptable salt thereof.

[076] Examples of other active ingredients that may be administered in combination with at least one compound of Formula I and/or at least one pharmaceutically acceptable salt thereof, and either administered separately or in the same pharmaceutical composition, include, but are not limited to: (a) other dipeptidyl peptidase IV (DPP-IV) inhibitors; (b) insulin sensitizers including (i) PPARy agonists such as the glitazones (e. g., troglitazone, pioglitazone, englitazone, MCC-555, rosiglitazone, balaglitazone, and the like) and other PPAR ligands, including PPARa/γ dual agonists, such as KRP-297 and muraglitazar, and PPARa agonists such as fenofibric acid derivatives (e.g., gemfibrozil, clofibrate, fenofibrate and bezafibrate), (ii) biguanides such as metformin and phenformin, and (iii) protein tyrosine phosphatase- IB (PTP- 1B) inhibitors; (c) insulin or insulin mimetics; (d) sulfonylureas and other insulin secretagogues, such as tolbutamide glyburide, glipizide, glimepiride, and meglitinides, such as nateglinide and repaglinide; (e) a-glucosidase inhibitors (such as acarbose and miglitol); (f) glucagon receptor antagonists such as those described in WO 98/04528, WO 99/01423, WO 00/39088, and WO 00/69810; (g) GLP-1, GLP-1 mimetics, such as Exendin 4, and liraglutide, and GLP-1 receptor agonists such as those described in WO00/42026 and WO00/59887 ; (h) GIP and GIP mimetics such as those described in WO00/58360, and GIP receptor agonists; (i) PACAP, PACAP mimetics, and PACAP receptor agonists such as those described in WO 01/23420 ; (j) cholesterol lowering agents such as (i) HMG-CoA reductase inhibitors (e.g., lovastatin, simvastatin, pravastatin, cerivastatin, fluvastatin, atorvastatin, itavastatin, and rosuvastatin, and other statins, as well as appropriate salts thereof), (ii) sequestrants (e.g., cholestyramine, colestipol, and dialkylaminoalkyl derivatives of a cross-linked dextran), (iii) nicotinyl alcohol, nicotinic acid or a salt thereof, (iv) PPARa agonists such as fenofibric acid derivatives (e.g., gemfibrozil, clofibrate, fenofibrate and bezafibrate), (v) PPARa/γ dual agonists, such as KRP- 297, (vi) inhibitors of cholesterol absorption, such as β-sitosterol and ezetimibe, (vii) acyl-CoA: cholesterol acyltransferase inhibitors, such as avasimibe, and (viii) anti-oxidants, such as probucol; (k) PPAR5 agonists, such as those described in W097/28149 ; (1) antiobesity compounds such as fenfluramine, dexfenflur amine, phentermine, sibutramine, orlistat, neuropeptide Yl or Y5 antagonists, CB1 receptor inverse agonists and antagonists, adrenergic receptor agonists, melanocortin-receptor agonists, for example, melanocortin-4 receptor agonists, ghrelin antagonists, and melanin-concentrating hormone (MCH) receptor antagonists; (m) ileal bile acid transporter inhibitors; (n) agents intended for use in inflammatory conditions such as aspirin, other non-steroidal anti-inflammatory drugs, such as ibuprofen, glucocorticoids, azulfidine, and selective cyclooxygenase-2 inhibitors; (o) antihypertensive agents such as ACE inhibitors (e.g., enalapril, lisinopril, captopril, quinapril, tandolapril), A-II receptor blockers (e.g., losartan, candesartan, irbesartan, valsartan, telmisartan, eprosartan), beta blockers and calcium channel blockers; and (p) glucokinase activators (GKAs).

[077] Dipeptidyl peptidase-IV inhibitors that can be combined with at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof include but are not limited to those described in WO 03/004498; WO 03/004496; EP 1 258 476; WO

02/083128; WO 02/062764; WO 03/000250; WO 03/002530; WO 03/002531 ; WO 03/002553; WO 03/002593; WO 03/000180; and WO 03/000181 .Exemplary mention can be made of DPP- IV inhibitor compounds such as isoleucine thiazolidide; MK-0431 ; and SYR-322.

[078] Antiobesity compounds that can be combined with at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof include but are not limited to fenfluramine, dexfenfluramine, phentermine, sibutramine, orlistat, neuropeptide Yl or Y5 antagonists, cannabinoid CB1 receptor antagonists or inverse agonists, melanocortin receptor agonists, for example, melanocortin-4 receptor agonists, ghrelin antagonists, and melanin- concentrating hormone (MCH) receptor antagonists. For a review of anti-obesity compounds that can be combined with compounds of structural formula I, see S. Chaki et al., "Recent advances in feeding suppressing agents: potential therapeutic strategy for the treatment of obesity, "Expert Opin. Ther. Patents, 11 : 1677-1692 (2001) and D. Spanswick and K. Lee, "Emerging antiobesity drugs, "Expert Opin. Emerging Drugs, 8: 217-237 (2003).

[079] Neuropeptide Y5 antagonists that can be combined with at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof include but are not limited to those described in U. S. Patent No. 6,335, 345 and WO 01/14376; and exemplary mention can be made of GW 59884A; GW 569180A ; LY366377; and CGP-71683A.

[080] Cannabinoid CB 1 receptor antagonists that can be combined with at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof include but are not limited to those described in PCT Publication WO 03/007887; U. S. Patent No. 5,624,941 , such as rimonabant; PCT Publication WO 02/076949, such as SLV-319; U. S. Patent No.

6,028,084; PCT Publication WO 98/41519; PCT Publication WO 00/10968; PCT Publication WO 99/02499 ; U. S. Patent No. 5,532,237; and U. S. Patent No. 5,292,736.

[081] Melanocortin receptor agonists that can be combined with at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof include but are not limited to those described in WO 03/009847; WO 02/068388; WO 99/64002; WO 00/74679; WO 01/70708; and WO 01/70337 as well as those described in J. D. Speake et al.,"Recent advances in the development of melanocortin-4 receptor agonists, "Expert Opin. Ther. Patents, 12: 1631-1638 (2002).

[082] The potential utility of safe and effective activators of glucokinase (GKAs) for the treatment of diabetes is discussed in J. Grimsby et al.,"Allosteric Activators of Glucokinase: Potential Role in Diabetes Therapy, "Science, 301 : 370-373 (2003).

[083] When at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein is used contemporaneously with one or more other drugs, in some embodiments, a pharmaceutical composition containing such at least one other drug in addition to the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein is used. Accordingly, the pharmaceutical compositions described herein include but are not limited to those that also contain one or more other active ingredients, in addition to at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein.

[084] The weight ratio of the at least one compound and/or at least one

pharmaceutically acceptable salt thereof described herein to the at least one second active ingredient may be varied and will depend upon the effective dose of each ingredient. For example, an effective dose of each will be used. Thus, for example, when at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein is combined with at least one another agent, the weight ratio of the at least one compound and/or at least one pharmaceutically acceptable salt thereof to the at least one another agent will for example range from 1000: 1 to 1 : 1000, such as from 200: 1 to 1 : 200. Combinations of at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein and other active ingredients will for example also be within the aforementioned range, but in each case, an effective dose of each active ingredient should be used.

[085] In such combinations the at least one compound and/or at least one

pharmaceutically acceptable salt thereof described herein and other active agents may be administered separately or in conjunction. In addition, the administration of one element may be prior to, concurrent to, or subsequent to the administration of other agent(s).

[086] The at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein may be administered by oral, parenteral (e. g., intramuscular, intraperitoneal, intravenous, ICV, intracisternal injection or infusion, subcutaneous injection, or implant), by inhalation spray, nasal, vaginal, rectal, sublingual, or topical routes of

administration and may be formulated, alone or together, in suitable dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants and vehicles appropriate for each route of administration. In addition to the treatment of warm- blooded animals such as mice, rats, horses, cattle, sheep, dogs, cats, monkeys, etc., the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein may be effective for use in humans.

[087] The pharmaceutical compositions for the administration of the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein may conveniently be presented in dosage unit form and may be prepared by any of the methods well known in the art of pharmacy. In some embodiments, the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein is brought into association with the carrier which constitutes one or more accessory ingredients.

[088] In some embodiments, the pharmaceutical compositions are prepared by uniformly and intimately bringing the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein into association with a liquid carrier or a finely divided solid carrier or both, and then, if necessary, shaping the product into the desired formulation. In some embodiments, the at least one compound and/or at least one

pharmaceutically acceptable salt thereof described herein is included in an amount sufficient to produce the desired effect upon the process or condition of diseases.

[089] The pharmaceutical compositions containing the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein may be in a form suitable for oral use, for example, as tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsions, hard or soft capsules, or syrups or elixirs.

[090] Compositions intended for oral use may be prepared according to any method known to the art for the manufacture of pharmaceutical compositions and such compositions may contain one or more agents selected from sweetening agents, flavoring agents, coloring agents and preserving agents in order to provide pharmaceutically elegant and palatable preparations. Tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients which are suitable for the manufacture of tablets. These excipients may be for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate, and sodium phosphate; granulating and disintegrating agents, for example, corn starch, and alginic acid; binding agents, for example starch, gelatin, and acacia, and lubricating agents, for example magnesium stearate, stearic acid, and talc. The tablets may be uncoated or they may be coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monostearate, and glyceryl distearate may be employed. They may also be coated by the techniques described in the U. S. Patent Nos. 4,256,108;

4,166,452; and 4,265,874 to form osmotic therapeutic tablets for controlled, such as sustained or delayed, release.

[091] Formulations for oral use may also be presented as hard gelatin capsules wherein the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate and/or kaolin, or as soft gelatin capsules wherein the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein is mixed with water or an oil medium, for example peanut oil, liquid paraffin, and/or olive oil. [092] Salts, such as sodium salts, of the DPP-IV inhibitors described herein may be prepared with carriers that protect the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein against rapid elimination from the body, such as time release formulations or coatings. The formulations may further include other active compounds to obtain desired combinations of properties.

[093] Oral pharmaceutical dosage forms may be as solid, gel or liquid. Examples of solid dosage forms include, but are not limited to tablets, capsules, granules, and bulk powders. More specific examples of oral tablets include compressed, chewable lozenges and tablets that may be enteric-coated, sugar-coated or film-coated. Examples of capsules include hard or soft gelatin capsules. Granules and powders may be provided in non-effervescent or effervescent forms. Each may be combined with other ingredients known to those skilled in the art.

[094] In certain embodiments, DPP-IV inhibitors described herein are provided as solid dosage forms, such as capsules and tablets. The tablets, pills, capsules, troches and the like may optionally contain one or more of the following ingredients, or compounds of a similar nature: a binder; a diluent; a disintegrating agent; a lubricant; a glidant; a sweetening agent; and a flavoring agent.

[095] Examples of binders that may be used include, but are not limited to,

microcrystalline cellulose, gum tragacanth, glucose solution, acacia mucilage, gelatin solution, sucrose and starch paste.

[096] Examples of lubricants that may be used include, but are not limited to, talc, starch, magnesium or calcium stearate, lycopodium and stearic acid.

[097] Examples of diluents that may be used include, but are not limited to, lactose, sucrose, starch, kaolin, salt, mannitol and dicalcium phosphate.

[098] Examples of glidants that may be used include, but are not limited to, colloidal silicon dioxide.

[099] Examples of disintegrating agents that may be used include, but are not limited to, crosscarmellose sodium, sodium starch glycolate, alginic acid, corn starch, potato starch, bentonite, methylcellulose, agar and carboxymethylcellulose.

[0100] Examples of coloring agents that may be used include, but are not limited to, any of the approved certified water soluble FD and C dyes, mixtures thereof; and water insoluble FD and C dyes suspended on alumina hydrate. [0101] Examples of sweetening agents that may be used include, but are not limited to, sucrose, lactose, mannitol and artificial sweetening agents such as sodium cyclamate and saccharin, and any number of spray-dried flavors.

[0102] Examples of flavoring agents that may be used include, but are not limited to, natural flavors extracted from plants such as fruits and synthetic blends of compounds that produce a pleasant sensation, such as, but not limited to peppermint and methyl salicylate.

[0103] Examples of wetting agents that may be used include, but are not limited to, propylene glycol monostearate, sorbitan monooleate, diethylene glycol monolaurate and polyoxyethylene lauryl ether.

[0104] Examples of anti-emetic coatings that may be used include, but are not limited to, fatty acids, fats, waxes, shellac, ammoniated shellac and cellulose acetate phthalates.

[0105] Examples of film coatings that may be used include, but are not limited to, hydroxyethylcellulose, sodium carboxymethylcellulose, polyethylene glycol 4000 and cellulose acetate phthalate.

[0106] If oral administration is desired, the at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof described herein may optionally be provided in a composition that protects it from the acidic environment of the stomach. For example, the composition can be formulated in an enteric coating that maintains its integrity in the stomach and releases the active compound in the intestine. The composition may also be formulated in combination with an antacid or other such ingredient.

[0107] When the dosage unit form is a capsule, it may optionally additionally comprise a liquid carrier such as a fatty oil. In addition, dosage unit forms may optionally additionally comprise various other materials that modify the physical form of the dosage unit, for example, coatings of sugar and other enteric agents.

[0108] The at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein may also be administered as a component of an elixir, suspension, syrup, wafer, sprinkle, chewing gum or the like. A syrup may optionally comprise, in addition to the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein, sucrose as a sweetening agent and certain preservatives, dyes and colorings and flavors.

[0109] The at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein may also be mixed with other active materials that do not impair the desired action, or with materials that supplement the desired action, such as antacids, H2 blockers, and diuretics. For example, if at least one compound and/or at least one

pharmaceutically acceptable salt thereof described herein is used for treating asthma or hypertension, it may be used with other bronchodilators and antihypertensive agents,

respectively.

[0110] Examples of pharmaceutically acceptable carriers that may be included in tablets comprising at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein include, but are not limited to binders, lubricants, diluents, disintegrating agents, coloring agents, flavoring agents, and wetting agents. Enteric-coated tablets, because of the enteric-coating, resist the action of stomach acid and dissolve or disintegrate in the neutral or alkaline intestines. Sugar- coated tablets may be compressed tablets to which different layers of pharmaceutically acceptable substances are applied. Film-coated tablets may be compressed tablets that have been coated with polymers or other suitable coating. Multiple compressed tablets may be compressed tablets made by more than one compression cycle utilizing the pharmaceutically acceptable substances previously mentioned. Coloring agents may also be used in tablets. Flavoring and sweetening agents may be used in tablets, and are especially useful in the formation of chewable tablets and lozenges.

[0111] Examples of liquid oral dosage forms that may be used include, but are not limited to, aqueous solutions, emulsions, suspensions, solutions and/or suspensions reconstituted from non-effervescent granules and effervescent preparations reconstituted from effervescent granules.

[0112] Examples of aqueous solutions that may be used include, but are not limited to, elixirs and syrups. As used herein, elixirs refer to clear, sweetened, hydroalcoholic preparations. Examples of pharmaceutically acceptable carriers that may be used in elixirs include, but are not limited to solvents. Particular examples of solvents that may be used include glycerin, sorbitol, ethyl alcohol and syrup. As used herein, syrups refer to concentrated aqueous solutions of a sugar, for example, sucrose. Syrups may optionally further comprise a preservative.

[0113] Emulsions refer to two-phase systems in which one liquid is dispersed in the form of small globules throughout another liquid. Emulsions may optionally be, but are not limited to, oil-in- water or water-in-oil emulsions. Examples of pharmaceutically acceptable carriers that may be used in emulsions include, but are not limited to non-aqueous liquids, emulsifying agents and preservatives.

[0114] Examples of pharmaceutically acceptable substances that may be used in non- effervescent granules, to be reconstituted into a liquid oral dosage form, include diluents, sweeteners and wetting agents.

[0115] Examples of pharmaceutically acceptable substances that may be used in effervescent granules, to be reconstituted into a liquid oral dosage form, include organic acids and a source of carbon dioxide.

[0116] Coloring and flavoring agents may optionally be used in all of the above dosage forms.

[0117] Exemplary examples of preservatives that may be used include glycerin, methyl and propylparaben, benzoic add, sodium benzoate and alcohol.

[0118] Exemplary examples of non-aqueous liquids that may be used in emulsions include mineral oil and cottonseed oil.

[0119] Exemplary examples of emulsifying agents that may be used include gelatin, acacia, tragacanth, bentonite, and surfactants such as polyoxyethylene sorbitan monooleate.

[0120] Exemplary examples of suspending agents that may be used include sodium carboxymethylcellulose, pectin, tragacanth, Veegum and acacia. Diluents include lactose and sucrose. Sweetening agents include sucrose, syrups, glycerin and artificial sweetening agents such as sodium cyclamate and saccharin.

[0121] Exemplary examples of wetting agents that may be used include propylene glycol monostearate, sorbitan monooleate, diethylene glycol monolaurate and polyoxyethylene lauryl ether.

[0122] Exemplary examples of organic acids that may be used include citric and tartaric acid.

[0123] Sources of carbon dioxide that may be used in effervescent compositions include sodium bicarbonate and sodium carbonate. Coloring agents include any of the approved certified water soluble ED and C dyes, and mixtures thereof.

[0124] Exemplary examples of flavoring agents that may be used include natural flavors extracted from plants such fruits, and synthetic blends of compounds that produce a pleasant taste sensation. [0125] For a solid dosage form, the solution or suspension, in for example propylene carbonate, vegetable oils or triglycerides, is, for example, encapsulated in a gelatin capsule. Such solutions, and the preparation and encapsulation thereof, are described in U.S. Patent Nos.

4,328,245; 4,409,239; and 4,410,545. For a liquid dosage form, the solution, e.g., for example, in a polyethylene glycol, may be diluted with a sufficient quantity of a pharmaceutically acceptable liquid carrier, e.g. water, to be easily measured for administration.

[0126] Alternatively, liquid or semi-solid oral formulations may be prepared by dissolving or dispersing the active compound or salt in vegetable oils, glycols, triglycerides, propylene glycol esters (e.g. propylene carbonate) and other such carriers, and encapsulating these solutions or suspensions in hard or soft gelatin capsule shells.

[0127] Also provided are compositions designed to administer the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein by parenteral administration, generally characterized by injection, either subcutaneous ly, intramuscularly or intravenously. Injectables may be prepared in any conventional form, for example as liquid solutions or suspensions, solid forms suitable for solution or suspension in liquid prior to injection, or as emulsions.

[0128] Examples of excipients that may be used in conjunction with injectables include, but are not limited to water, saline, dextrose, glycerol, and ethanol. The injectable compositions may also optionally comprise minor amounts of non-toxic auxiliary substances such as wetting or emulsifying agents, pH buffering agents, stabilizers, solubility enhancers, and other such agents, such as for example, sodium acetate, sorbitan monolaurate, triethanolamine oleate and cyclodextrins. Implantation of a slow-release or sustained-release system, such that a constant level of dosage is maintained (see, e.g., U.S. Patent No. 3,710,795) is also contemplated herein. The percentage of the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein contained in such parenteral compositions is highly dependent on the specific nature thereof, as well as the activity of the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein and the needs of the patient.

[0129] Parenteral administration of the formulations includes intravenous, subcutaneous and intramuscular administrations. Preparations for parenteral administration include sterile solutions ready for injection, sterile dry soluble products, such as the lyophilized powders described herein, ready to be combined with a solvent just prior to use, including hypodermic tablets, sterile suspensions ready for injection, sterile dry insoluble products ready to be combined with a vehicle just prior to use, and sterile emulsions. The solutions may be either aqueous or nonaqueous.

[0130] When administered intravenously, examples of suitable carriers include, but are not limited to physiological saline or phosphate buffered saline (PBS), and solutions containing thickening and solubilizing agents, such as glucose, polyethylene glycol, and polypropylene glycol and mixtures thereof.

[0131] Examples of pharmaceutically acceptable carriers that may optionally be used in parenteral preparations include, but are not limited to aqueous vehicles, nonaqueous vehicles, antimicrobial agents, isotonic agents, buffers, antioxidants, local anesthetics, suspending and dispersing agents, emulsifying agents, sequestering and chelating agents, and other

pharmaceutically acceptable substances.

[0132] Examples of aqueous vehicles that may optionally be used include Sodium Chloride Injection, Ringers Injection, Isotonic Dextrose Injection, Sterile Water Injection, Dextrose and Lactated Ringers Injection.

[0133] Examples of nonaqueous parenteral vehicles that may optionally be used include fixed oils of vegetable origin, cottonseed oil, corn oil, sesame oil and peanut oil.

[0134] Antimicrobial agents in bacteriostatic or fungistatic concentrations may be added to parenteral preparations, particularly when the preparations are packaged in multiple-dose containers and thus designed to be stored and multiple aliquots to be removed therefrom.

Examples of antimicrobial agents that may used include phenols and cresols, mercurials, benzyl alcohol, chlorobutanol, methyl and propyl p-hydroxybenzoic acid esters, thimerosal, benzalkonium chloride and benzethonium chloride.

[0135] Examples of isotonic agents that may be used include sodium chloride and dextrose. Examples of buffers that may be used include phosphate and citrate. Examples of antioxidants that may be used include sodium bisulfate. Examples of local anesthetics that may be used include procaine hydrochloride. Examples of suspending and dispersing agents that may be used include sodium carboxymethylcellulose, hydroxypropyl methylcellulose, and polyvinylpyrrolidone. Examples of emulsifying agents that may be used include Polysorbate 80 (TWEEN 80). A sequestering or chelating agent of metal ions includes EDTA. [0136] Pharmaceutical carriers may also optionally include ethyl alcohol, polyethylene glycol and propylene glycol for water miscible vehicles, and sodium hydroxide, hydrochloric acid, citric acid and lactic acid for pH adjustment.

[0137] The concentration of the at least one compound and/or at least one

pharmaceutically acceptable salt thereof described herein in the parenteral formulation may be adjusted so that an injection administers a pharmaceutically effective amount sufficient to produce the desired pharmacological effect. The exact concentration of the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein and/or dosage to be used will ultimately depend on the age, weight and condition of the patient or animal as is known in the art.

[0138] Unit-dose parenteral preparations may be packaged in an ampoule, a vial or a syringe with a needle. AU preparations for parenteral administration should be sterile, as is known and practiced in the art.

[0139] Injectables may be designed for local and systemic administration. For example, a therapeutically effective dosage is formulated to contain a concentration of at least about 0.1% w/w up to about 90% w/w or more, such as more than 1% w/w of the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein to the treated tissue(s). The at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein may be administered at once, or may be divided into a number of smaller doses to be administered at intervals of time. It is understood that the precise dosage and duration of treatment will be a function of the location of where the composition is parenterally administered, the carrier, and other variables that may be determined empirically using known testing protocols or by extrapolation from in vivo or in vitro test data. It is to be noted that concentrations and dosage values may also vary with the age of the individual treated. It is to be further understood that for any particular patient, specific dosage regimens may need to be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the formulations. Hence, the concentration ranges set forth herein are intended to be exemplary and are not intended to limit the scope or practice of the claimed formulations.

[0140] The at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein may optionally be suspended in micronized or other suitable form or may be derivatized to produce a more soluble active product. The form of the resulting mixture depends upon a number of factors, including the intended mode of administration and the solubility of the compound in the selected carrier or vehicle. The effective concentration is sufficient for ameliorating the symptoms of the disease state and may be empirically determined.

[0141] The at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein may also be prepared as lyophilized powders, which can be

reconstituted for administration as solutions, emulsions and other mixtures. The lyophilized powders may also be formulated as solids or gels.

[0142] Sterile, lyophilized powder may be prepared by dissolving the compound in a sodium phosphate buffer solution containing dextrose or other suitable excipient. Subsequent sterile filtration of the solution followed by lyophilization under standard conditions known to those of skill in the art provides the desired formulation. Briefly, the lyophilized powder may optionally be prepared by dissolving dextrose, sorbitol, fructose, corn syrup, xylitol, glycerin, glucose, sucrose, or other suitable agent, about 1-20%, such as about 5 to 15%, in a suitable buffer, such as citrate, sodium, and/or potassium phosphate and/or other such buffer known to those of skill in the art at, typically, about neutral pH. Then, the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein is added to the resulting mixture, for example, above room temperature, such as at about 30-35 °C, and stirred until it dissolves. The resulting mixture is diluted by adding more buffer to a desired concentration. The resulting mixture is sterile filtered or treated to remove particulates and to insure sterility, and apportioned into vials for lyophilization. Each vial may contain a single dosage or multiple dosages of the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein.

[0143] The at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein may also be administered as topical mixtures. Topical mixtures may be used for local and systemic administration. The resulting mixture may be a solution, suspension, emulsion, or the like and is formulated as creams, gels, ointments, emulsions, solutions, elixirs, lotions, suspensions, tinctures, pastes, foams, aerosols, irrigations, sprays, suppositories, bandages, dermal patches, or any other formulations suitable for topical administration. The at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein may be formulated as aerosols for topical application, such as by inhalation (see, U.S. Patent Nos. 4,044,126, 4,414,209, and 4,364,923, which describe aerosols for delivery of a steroid useful for treatment inflammatory diseases, particularly asthma). These formulations for administration to the respiratory tract can be in the form of an aerosol or solution for a nebulizer, or as a micro fine powder for insufflation, alone or in combination with an inert carrier such as lactose. In such a case, the particles of the formulation will for example have median diameters of less than 50 microns, such as less than 10 microns.

[0144] The at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein may also be formulated for local or topical application, such as for topical application to the skin and mucous membranes, such as in the eye, in the form of gels, creams, and lotions and for application to the eye or for intracisternal or intraspinal application. Topical administration is contemplated for transdermal delivery and also for administration to the eyes or mucosa, or for inhalation therapies. Nasal solutions of the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein alone or in combination with other pharmaceutically acceptable excipients can also be administered.

[0145] Depending upon the disease state being treated, other routes of administration, such as topical application, transdermal patches, and rectal administration, may also be used. For example, pharmaceutical dosage forms for rectal administration are rectal suppositories, capsules and tablets for systemic effect. Rectal suppositories are used herein mean solid bodies for insertion into the rectum that melt or soften at body temperature releasing one or more pharmacologically or therapeutically active ingredients. Pharmaceutically acceptable substances utilized in rectal suppositories are bases or vehicles and agents to raise the melting point.

Examples of bases include cocoa butter (theobroma oil), glycerin-gelatin, carbowax,

(polyoxyethylene glycol) and appropriate mixtures of mono-, di- and triglycerides of fatty acids. Combinations of the various bases may be used. Agents to raise the melting point of

suppositories include spermaceti and wax. Rectal suppositories may be prepared either by the compressed method or by molding. The typical weight of a rectal suppository is about 2 to 3 gm. Tablets and capsules for rectal administration may be manufactured using the same

pharmaceutically acceptable substance and by the same methods as for formulations for oral administration. [0146] In the treatment of conditions which require inhibition of dipeptidyl peptidase-IV enzyme activity an appropriate dosage level will generally be about 0.1 to 1000 mg per day which can be administered in single or multiple doses.

[0147] For example, the dosage level will be about 0.1 to about 250 mg per day; such as from about 0.5 to about 100 mg per day. A suitable dosage level may be about 0.1 to 1000 mg per day, about 0.5 to 500 mg per day, or about 1 to 50 mg per day. Within this range the dosage may be 0.1 to 0.5, 0.5 to 5 or 5 to 50 mg per day. For oral administration, the compositions are for example provided in the form of tablets containing 1.0 to 1000 mg of the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein, such as 1.0, 5.0, 10.0, 15.0. 20.0, 25.0, 50.0, 75.0, 100.0, 150.0, 200.0, 250.0, 300.0, 400.0, 500.0, 600.0, 750.0, 800.0, 900.0, and 1000.0 mg of the at least one compound and/or at least one

pharmaceutically acceptable salt thereof described herein for the symptomatic adjustment of the dosage to the patient to be treated. The at least one compound and/or at least one

pharmaceutically acceptable salt thereof described herein may be administered on a regimen of 1 to 4 times per day, such as once or twice per day.

[0148] When treating diabetes mellitus and/or hyperglycemia or hypertriglyceridemia or other diseases for which the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein are indicated, generally satisfactory results may be obtained when the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein are administered at a daily dosage of, for example, from about 0.1 mg to about 3000 mg , for example given as a single daily dose or in divided doses two to six times a day, or in sustained release form. For most large mammals, the total daily dosage may be from about 1.0 mg to about 1000 mg, such as from about 1 mg to about 50 mg. In the case of a 70 kg adult human, the total daily dose may generally be from about 7 mg to about 350 mg. This dosage regimen may be adjusted to provide the optimal therapeutic response.

[0149] It will be understood, however, that the specific dose level and frequency of dosage for any particular patient may be varied and will depend upon a variety of factors including the activity of the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein employed, the metabolic stability and length of action of that at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein, the age, body weight, general health, sex, diet, mode and time of administration, rate of excretion, drug combination, the severity of the particular condition, and the host undergoing therapy.

[0150] Inhibition constants may, for example, be determined as follows. A continuous fiuorometric assay is employed with the substrate Gly-Pro-AMC, which is cleaved by DPP-IV to release the fluorescent AMC leaving group. A typical reaction contains approximately 50 pM enzyme, 50 μΜ Gly-Pro-AMC, and buffer (100 mM HEPES, pH 7.5, 0.1 mg/ml BSA) in a total reaction volume of 100 μL·. Liberation of AMC is monitored continuously in a 96-well plate fluorometer using an excitation wavelength of 360 nm and an emission wavelength of 460 nm. Under these conditions, approximately 0.8 μΜ AMC is produced in 30 minutes at 25 degrees C. The enzyme used in these studies was soluble (transmembrane domain and cytoplasmic extension excluded) human protein produced in a baculovirus expression system (Bac-To-Bac, Gibco BRL). The kinetic constants for hydrolysis of Gly-Pro-AMC and GLP-1 were found to be in accord with literature values for the native enzyme. To measure the dissociation constants for the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein, solutions of the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein in DMSO were added to reactions containing enzyme and substrate (final DMSO concentration is 1%). All experiments were conducted at room temperature using the standard reaction conditions described above. To determine the dissociation constants (Ki), reaction rates were fit by non-linear regression to the Michaelis-Menton equation for competitive inhibition. The errors in reproducing the dissociation constants are typically less than two-fold.

[0151] For example, the at least one compound and/or at least one pharmaceutically acceptable salt thereof of the following examples had activity in inhibiting the dipeptidyl peptidase-IV enzyme in the aforementioned assays, generally with an IC₅₀ of less than about 1 μΜ. Such a result is indicative of the intrinsic activity of the at least one compound and/or at least one pharmaceutically acceptable salt thereof described herein in use as inhibitors of the dipeptidyl peptidase-IV enzyme activity.

[0152] Several methods for preparing the at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof are illustrated in the following Schemes and Examples without limiting the scope of the present disclosure. Starting materials are made according to procedures known in the art or as illustrated herein. EXAMPLES

Preparation of DPP-IV Inhibitors

[0153] Various methods may be developed for synthesizing the at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof. Representative methods for synthesizing the at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof are provided in the Examples. It is noted, however, that the at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof may also be synthesized by other synthetic routes that others may devise.

[0154] It will be readily recognized that certain compounds of formula I have atoms with linkages to other atoms that confer a particular stereochemistry to the compound (e.g., chiral centers). It is recognized that synthesis of the at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof may result in the creation of mixtures of different stereoisomers (enantiomers, diastereomers). Unless a particular stereochemistry is specified, recitation of a compound is intended to encompass all of the different possible stereoisomers.

[0155] The at least one compound of formula (I) can also be prepared as a

pharmaceutically acceptable acid addition salt by, for example, reacting the free base form of the at least one compound with a pharmaceutically acceptable inorganic or organic acid.

Alternatively, a pharmaceutically acceptable base addition salt of the at least one compound of formula (I) can be prepared by, for example, reacting the free acid form of the at least one compound with a pharmaceutically acceptable inorganic or organic base. Inorganic and organic acids and bases suitable for the preparation of the pharmaceutically acceptable salts of compounds of formula (I) are set forth in the definitions section of this Application.

Alternatively, the salt forms of the compounds of formula (I) can be prepared using salts of the starting materials or intermediates.

[0156] The free acid or free base forms of the compounds of formula (I) can be prepared from the corresponding base addition salt or acid addition salt form. For example, a compound of formula (I) in an acid addition salt form can be converted to the corresponding free base thereof by treating with a suitable base (e.g., ammonium hydroxide solution, sodium hydroxide, and the like). A compound of formula (I) in a base addition salt form can be converted to the

corresponding free acid thereof by, for example, treating with a suitable acid (e.g., hydrochloric acid, etc). [0157] The N-oxides of the at least one compound of formula (I) and/or at least one pharmaceutically acceptable salt thereof can be prepared by methods known to those of ordinary skill in the art. For example, N-oxides can be prepared by treating an unoxidized form of the compound of formula (I) with an oxidizing agent (e.g., trifluoroperacetic acid, permaleic acid, perbenzoic acid, peracetic acid, meta-chloroperoxybenzoic acid, or the like) in a suitable inert organic solvent (e.g., a halogenated hydrocarbon such as dichloromethane) at approximately 0 °C. Alternatively, the N-oxides of the compounds of formula (I) can be prepared from the N-oxide of an appropriate starting material.

[0158] Compounds of formula (I) in an unoxidized form can be prepared from N-oxides of compounds of formula (I) by, for example, treating with a reducing agent (e.g., sulfur, sulfur dioxide, triphenyl phosphine, lithium borohydride, sodium borohydride, phosphorus trichloride, tribromide, and the like) in an suitable inert organic solvent (e.g., acetonitrile, ethanol, aqueous dioxane, and the like) at 0 to 80 °C.

[0159] Protected derivatives of the compounds of formula (I) can be made by methods known to those of ordinary skill in the art. A detailed description of the techniques applicable to the creation of protecting groups and their removal can be found in T.W. Greene, Protecting Groups in Organic Synthesis, 3rd edition, John Wiley & Sons, Inc. 1999.

[0160] The at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof may be conveniently prepared, or as solvates (e.g. hydrates). Hydrates of the at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof may be conveniently prepared by recrystallization from an aqueous/organic solvent mixture, using organic solvents such as dioxin, tetrahydrofuran and/or methanol.

[0161] The compounds of formula (I) can also be prepared as their individual

stereoisomers by reacting a racemic mixture of the compounds with an optically active resolving agent to form a pair of diastereoisomeric compounds, separating the diastereomers and recovering the optically pure enantiomer. While resolution of enantiomers can be carried out using covalent diasteromeric derivatives of compounds, dissociable complexes are preferred (e.g., crystalline diastereoisomeric salts). Diastereomers have distinct physical properties (e.g., melting points, boiling points, solubilities, reactivity, etc.) and can be readily separated by taking advantage of these dissimilarities. The diastereomers can be separated by chromatography or, for example, by separation/resolution techniques based upon differences in solubility. The optically rC50C pure enantiomer is then recovered, along with the resolving agent, by any practical means that would not result in racemization. A more detailed description of the techniques applicable to the resolution of stereoisomers of compounds from their racemic mixture can be found in Jean Jacques Andre Collet, Samuel H. Wilen, Enantiomers, Racemates and Resolutions, John Wiley & Sons, Inc. (1981).

[0162] As used herein the symbols and conventions used in these processes, schemes and examples are consistent with those used in the contemporary scientific literature, for example, the Journal of the American Chemical Society or the Journal of Biological Chemistry. Standard single-letter or three-letter abbreviations are generally used to designate amino acid residues, which are assumed to be in the L-configuration unless otherwise noted. Unless otherwise noted, all starting materials were obtained from commercial suppliers and used without further purification. For example, the following abbreviations may be used in the examples and throughout the specification: g (grams); mg (milligrams); L (liters); mL (milliliters); μL

(microliters); psi (pounds per square inch); M (molar); mM (millimolar); i.v. (intravenous); Hz (Hertz); MHz (megahertz); mol (moles); mmol (millimoles); RT (room temperature); min (minutes); h (hours); mp (melting point); TLC (thin layer chromatography); Tr (retention time); RP (reverse phase); MeOH (methanol); i-PrOH (isopropanol); TEA (triethylamine); TFA (trifiuoroacetic acid); TFAA (trifiuoroacetic anhydride); THF (tetrahydrofuran); DMSO

(dimethylsulfoxide); EtOAc (ethyl acetate); DME (1 ,2-dimethoxyethane); DCM

(dichloromethane); DCE (dichloroethane); DMF ( ,Ν-dimethylformamide); DMPU (Ν,Ν'- dimethylpropyleneurea); CDI (1,1-carbonyldiimidazole); IBCF (isobutyl chloro formate); HO Ac (acetic acid); HOSu (N-hydroxysuccinimino); HOBT (1 -hydro xybenzotriazole); Et₂0 (diethyl ether); EDCI (l-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride); BOC (tert- butyloxycarbonyl); FMOC (9-fluorenylmethoxycarbonyl); DCC (dicyclohexylcarbodiimino); CBZ (benzyloxycarbonyl); Ac (acetyl); atm (atmosphere); TMSE (2-(trimethylsilyl)ethyl); TMS (trimethylsilyl); TIPS (triisopropylsilyl); TBS (t-butyldimethylsilyl); DMAP (4- dimethylaminopyridine); Me (methyl); OMe (methoxy); Et (ethyl); Et (ethyl); tBu (tert-butyl); HPLC (high pressure liquid chomatography); BOP (bis(2-oxo-3-oxazolidinyl)phosphinic chloride); TBAF (tetra-n-butylammonium fluoride); mCPBA (meta-chloroperbenzoic acid).

[0163] References to ether or Et₂0 are to diethyl ether; brine refers to a saturated aqueous solution of NaCl. Unless otherwise indicated, all temperatures are expressed in °C (degrees Centigrade). All reactions were conducted under an inert atmosphere at RT unless otherwise noted.

[0164] 1H NMR spectra were recorded on a Varian Mercury Plus 400. Chemical shifts are expressed in parts per million (ppm). Coupling constants are in units of hertz (Hz). Splitting patterns describe apparent multiplicities and are designated as s (singlet), d (doublet), t (triplet), q (quartet), m (multiplet), and br (broad).

[0165] Low-resolution mass spectra (MS) and compound purity data were acquired on a Shimadzu LC/MS single quadrapole system equipped with electrospray ionization (ESI) source, UV detector (220 and 254 nm), and evaporative light scattering detector (ELSD). Thin-layer chromatography was performed on 0.25 mm E. Merck silica gel plates (60F- 254), visualized with UV light, 5% ethanolic phosphomolybdic acid, Ninhydrin or p-anisaldehyde solution. Flash column chromatography was performed on silica gel (230-400 mesh, Merck).

Synthetic Schemes

[0166] The at least one compound of formula I and/or at least one pharmaceutically acceptable salt thereof may be synthesized according to a variety of reaction schemes. Some illustrative schemes are provided herein in the examples. Other reaction schemes could be readily devised by those skilled in the art.

[0167] In the reactions described hereinafter it may be necessary to protect reactive functional groups, for example hydroxy, amino, imino, thio or carboxy groups, where these are desired in the final product, to avoid their unwanted participation in the reactions. Conventional protecting groups may be used in accordance with standard practice, for examples see T.W. Greene and P. G. M. Wuts in "Protective Groups in Organic Chemistry" John Wiley and Sons, 1991.

[0168] Synthetic methods for preparing the compounds of the present invention are illustrated in the following Schemes and Examples. Starting materials are commercially available or may be made according to procedures known in the art or as illustrated herein.

[0169] The compound of formula I of the present invention may be prepared from intermediates such as those of formula III and amino-heteroarene such as II using standard condensation conditions followed by reduction and chiral resolution.

[0170] The preparation of these intermediates is described in the following Schemes, wherein Ar, X and Y are as defined above and Z is a halogen such as CI, Br or I. Compounds of formula Ilia may be prepared from intermediate 3 using a route as illustrated in Scheme 1. Intermediate 4 may be prepared by a method described in Leon W.A van Berko et ah,

Tetrahedron Lett., 1988, 44, 5091-5094. Reaction of arylaldehyde (1) with nitromethane in the presence of a base such as NaOH followed by quenching the reaction mixture with HCl-ZnCl₂ provides the nitrostyrene intermediate 2. Diels-Alder reaction of nitrostyrene 2 with 2- methoxybuta- 1 ,3-diene (3) provide cyclic methyloxy enol ether 4. Intermediate Ilia may be obtained by bromination reaction of 4 with halogenating reagents such as NBS.

Scheme 1

1 2 4 Ilia

[0171] Compounds of formula I may be prepared by condensation reactions from intermediate Ilia and intermediate II, which are either known in the literature or may be prepared by a variety of methods familiar to those skilled in the art (Scheme 2). Intermediate 5 may be prepared by reacting intermediates Ilia and II in solvents such as ethanol under elevated temperature. Reduction of the nitro group of 5 with reducing agents (for example, zinc dust in a solvent such as acetic acid) provides the compound of formula I. Coupling of compound I with Z⁾-mandelic acid provides amides 6a and 6b as a mixture of two diasteromers. Amide 6a was separated from 6b by recrystallization in solvent such as dichloromethane. Cleavage of the amide bonds in 6a and 6b in acids such as HC1 provides single enatiomers of 7a and 7b, respectively. Reduction of the six-membered heteroaryl ring in 7a and 7b using conditions such as catalytic hydrogenations gives product la and lb, respectively.

SCHEME 2

6b

7a la

7b lb

[0172] The compound of formula I or synthetic intermediates illustrated in the above schemes may be further modified, for example, by manipulation of substituents on X or Y. These manipulations may include, but are not limited to, oxidation, alkylation, arylation, acylation, and hydrolysis reactions that are commonly known to those skilled in the art.

[0173] One such example is illustrated in Scheme 3. Amine 8 may be prepared by reaction of Ilia and 2-amino-pyrazine using conditions described in Scheme 2. Protection of amine in 8 with amino protecting group such as Boc gives compound 9. Hydrogenation of 9 with Pd/C provides amine 10. Treatment of 10 with CDI in solvent such as toluene followed by reaction with NaOMe gives methyl carbamate 11. Deprotection of Boc group in 11 with an acid such as hydrogen chloride in a solvent such as ethyl acetate provides compound 12.

SCHEME 3

11

12

[0174] In some cases the order of carrying out the foregoing reaction schemes may be varied to facilitate the reaction or to avoid unwanted reaction products. The following examples are provided so that the invention might be more fully understood. These examples are illustrative only and should not be construed as limiting the invention in any way.

INTERMEDIATE A

2-Bromo-4-nitro-5-(2,4,5-trifluorophenyl)cvclohexanone

Step A. (iT)-l ,2,4-trifluoro-5-(2-nitrovinyl)benzene

[0175] To a solution of MeOH (600 mL), water (300 mL), and 2.5 N NaOH (300 mL) at 5 C was added a solution of 2,4,5 -trifluorobenzaldehyde (100 g, 0.6246 mol) and MeN0₂ (40 mL, 0.750 mol) in MeOH (100 mL) dropwise over 30-60 min, while the internal temperature was maintained between 5 and IO C with external cooling. The reaction solution was then agitated between 0 and 5 C for an additional 30 min, maintained between 0 and 5 C, and added dropwise to a solution of ZnCl₂ (426 g, 3.123 mol) in cone. HC1 (130 mL, 1.562 mol) and water (170 mL) at 0-10 C with vigorous agitation over 2-4 h. The light yellow product precipitated during the addition. After addition, the slurry was allowed to warm to room temperature and stand for 1 h before filtration. The wet cake was washed with 40% MeOH in water (300 mL X 3). The wet cake was suction dried at room temperature to give the title compound as a light yellow product. 1H NMR (400 MHz, CDC1₃) δ 7.96 (d, J = 13.8 Hz, 1 H), 7.65 (d, J = 13.8 Hz, 1 H), 7.37 (m, 1 H), 7.09 (m, 1 H).

Step B. 2-Methoxybuta-1.3-diene 3)

[0176] To a solution of methyl vinyl ketone (50 g, 713 mmol) and trimethoxymethane (68.82 g, 648 mmol) in anhydrous MeOH (65.6 mL) was added 4N HC1 in dioxane (0.44 mL) under cooling of water. Upon completion of addition, the mixture was stirred at room

temperature for 7 days. The mixture was refluxed for 30 min and then cooled to room

temperature. After neutralization with K₂C0₃, the mixture was filtered and the filtrate was fractionally distilled under vacuum to give 1 ,3,3-trimethoxybutane.

[0177] 1,3,3-Trimethoxybutane was dropped slowly into a heated distilling flask containing 0.1 g of potassium bisulfate. The 1 ,3,3-trimethoxy compound was added at such a rate that approximately 20 g was present in the flask. The temperature of the flask was maintained at 140 °C to cause steady distillation of the decomposition products, . e., methyl alcohol and 2- methoxybuta-l ,3-diene. The decomposition products were collected in 5% potassium carbonate solution and by further washing with potassium carbonate solution followed by drying and distilling. From 70 g of the 1 ,3,3-trimethoxy compound there was obtained in this manner 23 g of 2-methoxybuta-l ,3-diene (58% conversion)..

Step C. 1 ,2,4-Trifluoro-5-(3-methoxy-6-nitrocvclohex-3-enyl)benzene

[0178] A mixture of 2-methoxybuta-l ,3-diene (3, 109 g, 1.296 mol) and (E)- 1 ,2,4- trifluoro-5-(2-nitrovinyl)benzene (87.73 g, 0.432 mol) was stirred in a sealed tube at 120 °C for 20 hrs. The mixture was cooled to room temperature and then filtered. The filter cake was rinsed with MeOH until it was colorless to provide the title compound. The filtrate was concentrated under vacuum. The residue was subjected to a silica gel plug filtration, eluting with 25% 50C dichloromethane-hexane. After concentration, the residue was suspended in MeOH, additional title compound was collected after filtration.

Step D. 2-Bromo-4-nitro-5-(2,4,5-trifluorophenyl)cyclohexanone

[0179] To a solution of NBS (52.15 g, 0.293 mol) in 1.6 L of THF/water (1 : 1) was added NaOAc (2.0 g, 0.024 mol) and the product of step C (70.19 g, 0.244 mol) at 0 °C. The mixture was stirred at that temperature for 30 min. Then the mixture was concentrated under vacuum at 10 °C. The residue was diluted with ethyl acetate, washed with saturated aqueous Na₂S₂0₃, saturated aqueous NaHC0₃ and saturated aqueous brine successively, and then concentrated under vacuum. The residue was dissolved in minimum volume of DCM and precipitated by addition of hexane. After filtration, the title compound was collected. ^!H NMR (CD₃C1, 400 MHz): δ 7.04 (m, 2H), 5.57 (m, 1H), 4.52 (br, 1H), 3.78 (t, 1H), 3.57 (t, 1H), 2.90 (m, 2H), 2.62 (d, 1H).

INTERMEDIATE B

2-Bromo-5-(2,5-difluorophenyl)-4-nitrocvclohexanone

[0180] The title compound was prepared as described above for Intermediate A, starting with 2,5-difluorobenzaldehyde.

EXAMPLE 1

(7_tS',8^)-8-(2.4.5-trifluorophenyl)-1.2.3.4.6.7.8.9-octahvdrobenzor4.51imidazori .2- alpyrazin-7-amine

Step A. 7-nitro-8-(2,4,5-trifluorophenyl)-6,7,8,9-tetrahydrobenzo[4,51imidazo[l,2- alpyrazine

[0181] 2-Bromo-4-nitro-5-(2,4,5-trifluorophenyl)cyclohexanone_(Intermediate A) (1.056 g, 3 mmol) was suspended in EtOH (25 ml) in a sealed tube. The suspension was heated to 80 C to obtain a clear solution, and then 2-aminopyrazine (288 mg, 3 mmol) in EtOH (5 ml) was added. The mixture was stirred at 100 C for 18 hrs and then the solvent was removed in vacuo. The residue was diluted with EtOAc and H₂0, adjusted to pH = 8 with saturated aqueous NaHC0₃ solution, and extracted with EtOAc (60 ml x 3). The combined extracts were washed with brine, dried over anhydrous Na₂SO/_t, and concentrated. The mixture was separated by column chromatography on silica gel, eluted with 1 :2.5 to 1 :5 petroleum ether-ethyl acetate to give 470 mg of the title compound.. MS-ESI (m/z): 349 [M+l]⁺.

Step B. 8-(2,4,5-trifluoroDhenyl)-6,7,8,9-tetrahydrobenzo[4,51imidazo[l ,2-alpyrazin-7- amine

[0182] A mixture of 7-nitro-8-(2,4,5-trifluorophenyl)-6,7,8,9- tetrahydrobenzo[4,5]imidazo[l,2-a]pyrazine (from Step A, 11 g, 31.6 mmol) and SnCl₂-2H₂0 (28.5 g, 126 mmol)) in EtOAc was refluxed overnight. The mixture was cooled to room temperature and then purified by column chromatography on silica gel to give 6.7 g of the title compound as a reddish brown solid. MS-ESI (m/z): 319 [M+l]⁺.

Step C. (^)-2-hvdroxy-2-phenyl-N-((7i?,8_tS^r)-8-(2.4.5-trifluorophenyl)-6.7.8.9- tetrahydrobenzo[4.51imidazo[1.2-alpyrazin-7-yl)acetamide

[0183] A mixture of 8-(2,4,5-trifluorophenyl)-6,7,8,9-tetrahydrobenzo[4,5]imidazo[l,2- a]pyrazin-7-amine (from Step B, 6.1 g, 19.2 mmol ), Z)-mandelic acid (4.4 g, 28.8 mmol ), HOBT (3.9 g, 28.8 mmol), EDCI (5.5 g, 28.8 mmol ), and DIPEA (13.3 mL, 76.7 mmol) in DMF was stirred at room temperature overnight. The mixture was poured into ice-water under vigorous stirring. The solid was collected by filtration. The filter cake was suspended in dichloromethane and filtered to give 1.25 g of title compound as an off-white solid. MS-ESI (m/z): 453 [M+l]⁺ .

Step D. (7_tS',8i?)-8-(2.4.5-trifluorophenyl)-6.7.8.9-tetrahvdrobenzor4.51imidazori .2- alpyrazin-7-amine

[0184] A mixture of (ii)-2-hydroxy-2-phenyl-N-((7ii,S_l )-8-(2,4,5-trifiuorophenyl)- 6,7,8,9-tetrahydrobenzo[4,5]imidazo[l,2-a]pyrazin-7-yl)acetamide (from Step C. 1.25 g, 2.77 mmol) and 6 N HC1 aq. soln. was refluxed overnight. The mixture was cooled to room temperature and then adjusted to pH = 12 with 8 N NaOH aq. soln. The mixture was extracted with EtOAc, and the extracts were dried and then concentrated under vacuum to give 1.0 g the title compound as an off-white solid. MS-ESI (m/z): 319 [M+l]⁺ . Step E. (7_tS',8^V8-(2.4.5-trifluorophenylV1.2.3.4.6.7.8.9-octahvdrobenzor4.51imidazori .2- alpyrazin-7-amine

[0185] A suspension of (7 8i¾-8-(2,4,5-trifluorophenyl)-6,7,8,9- tetrahydrobenzo[4,5]imidazo[l ,2-a]pyrazin-7-amine (from Step D, 0.9 g, 2.80 mmol), 10% Pd/C, (50mg) and HCO₂NH₄ (1.76 g, 28 mmol ) in MeOH was stirred at room temperature overnight. The mixture was filtered, and the filtrate was concentrated under vacuum. To the residue of concentration was added water, and then it was extracted with EtOAc. The extracts were washed with water, dried over anhydrous Na₂S0₄, and then concentrated under vacuum to give 760 mg of the title compound as a yellowish white foam. 1H NMR (400 MHz, D₂0, ppm): δ 7.34 (m, 1H), 7.18 (m, 1H), 4.42 (br, 2H), 4.1 1 (m, 1H), 3.85 (br, 2H), 3.65 (m, 1H), 3.33 (d, 1H), 3.09 (m, 3H), 2.93 (t, 1H) 2.01 (s, 1H); MS-ESI (m/z): 323 [M+l ]⁺.

EXAMPLE 2

(7R,^)-7-(2.4.5-trifluorophenyl)-1.2.3.4.6.7.8.9-octahvdrobenzor4.51imidazori .2- alpyridin-8-amine

[0186] This compound was prepared by substituting 2-amino-pyrazine with 2-amino- pyridine in EXAMPLE 1. MS-ESI (m z): 322 [M+l ]⁺.

EXAMPLE 3

(7_tS',8^)-methyl 7-amino-8-(2.4.5-trifluorophenyl)-3.4.6.7.8.9- hexahydrobenzo[4,51imidazo[l ,2-alpyrazine-2(lH)-carboxylate bis-hydrochloric acid salt

Step A. fe -Butyl ((7_ty,8i?)-8-(2.4.5-trifluorophenyl)-6.7.8.9- tetrahydrobenzo[4,51imidazo[l ,2-alpyrazin-7-yl)carbamate

[0187] To a solution of (7S,8i¾-8-(2,4,5-trifiuorophenyl)-6,7,8,9- tetrahydrobenzo[4,5]imidazo[l ,2-a]pyrazin-7-amine_(from Example 1 , Step D, 1 g, 2.3 mmol) in THF was added Boc₂0 (0.6 g, 2.76 mmol ) and NaHC0₃ (0.39 g, 4.6 mmol) at 0 °C. The mixture was stirred at 0 °C for 5 hrs. The mixture was poured into water and then extracted with EtOAc. The extracts were washed with water, dried over anhydrous a₂S0₄, and then concentrated under vacuum. The residue was purified by column chromatography to give 0.9 g of the title compound as an off-white foam. MS-ESI (m/z): 419 [M+l ]⁺ .

Step B. fe -Butyl ((7£8i?)-8-(2.4.5-trifluorophenyl)-l .2.3.4.6.7.8.9- octahydrobenzo[4,51imidazo[ 1 ,2-alpyrazin-7-yl)carbamate

[0188] A suspension of ri-butyl ((75,8R)-8-(2,4,5-trifluorophenyl)-6,7,8,9- tetrahydrobenzo[4,5]imidazo[l ,2-a]pyrazin-7-yl)carbamate (from Step A, 0.9 g, 2.15 mmol), 10% Pd/C (270 mg), and HC0₂NH₄ (1.35 g, 21.5 mmol) in MeOH was stirred at room temperature overnight. The mixture was filtered, and the filtrate was concentrated under vacuum. To the residue of concentrate was added water, and then it was extracted with EtOAc. The extracts were washed with water, dried over anhydrous Na₂SO/t, and then concentrated under vacuum to give 760 mg the title compound as a yellowish white foam. MS-ESI (m/z): 423

[M+l]⁺.

Step C. (7S,8R)-met y\ 7-((?gr?-butoxycarbonyl)amino)-8-(2.4.5-trifluorophenyl)- 3,4,6,7, 8,9-hexahydrobenzo[4,51imidazo[l ,2-alpyrazine-2(lH)-carboxylate

[0189] A mixture of ri-butyl ((75,8R)-8-(2,4,5-trifluorophenyl)-l ,2,3,4,6,7,8,9- octahydrobenzo[4,5]imidazo[l ,2-a]pyrazin-7-yl)carbamate (from Step B, 760 mg, 1.8 mmol) and CDI (584 mg, 3.6 mmol) in toluene was stirred at room temperature for an hour. To the mixture was added a solution of 2.1 N MeONa in MeOH (1.7 mL, 3.6 mmol), and it was stirred at room temperature for half an hour. To the mixture was added water, followd by extraction with EtOAc (10 ml 3). The extracts were washed with water, dried over anhydrous Na₂SO/t, concentrated, and then purified by column chromatography to give 605 mg of the title compound as an off- white foam. MS-ESI (m/z): 481 [M+1]⁺.

Step D. (75.8RVMethyl 7-amino-8-(2.4.5-trifluorophenyl)-3.4.6.7.8.9- hexahydrobenzo[4,51imidazo[l ,2-alpyrazine-2(lH)-carboxylate bis-hydrochloric acid salt

[0190] To a solution of (75',S^?)-methyl 7-((tert-butoxycarbonyl)amino-8-(2,4,5- trifluorophenyl)-3,4,6,7,8,9-hexahydrobenzo[4,5]imidazo[l ,2-a]pyrazine-2(lH)-carboxylate (from Step C, 605 mg, 1.26 mmol) in EtOAc was added a solution of 4N HC1 in EtOAc. The mixture was stirred at room temperature for an hour. Concentration under vacuum gave 560 mg of the title compound as an off-white solid. 1H NMR (400 MHz, D₂0): δ 7.32 (s, 1H), 7.18 (s, 1H), 4.91 (s, 2H), 4.08 (s, 3H), 3.96 (s, 2H), 3.72 (s, 3H), 3.63 (s, 1H), 3.25 (d, 1H), 3.09 (m, 2H), 2.88 (t, 1H);MS-ESI (m/z): 381 [M+l]⁺.

[0191]

EXAMPLE 4

(7_ty,8i?Vmethyl 7-amino-8-(2.5-difluorophenylV3.4.6.7.8.9- hexahydrobenzo[4,51imidazo[l ,2-alpyrazine-2(lH)-carboxylate bis-hydrochloric acid salt

[0192] This compound was prepared by following Steps A to D in EXAMPLE 1 , substituting Intermediate A with Intermediate B, and following the procedures in EXAMPLE 3. MS-ESI (m/z): 363 [M+l]⁺.

EXAMPLE 5

(7_tS',8i?V2-methyl-8-(2.4.5-trifluorophenylV1.2.3.4.6.7.8.9-octahvdrobenzor4.51imidazori .2- alpyrazin-7-amine bis-hydrochloric acid salt

[0193] A solution of ri-butyl ((7, ,Sii)-8-(2,4,5-trifiuorophenyl)-l ,2,3,4,6,7,8,9- octahydrobenzo[4,5]imidazo[l ,2-a]pyrazin-7-yl)carbamate (from EXAMPLE 3, Step B, 21 mg, 0.05 mmol), 37% aq. formaldehyde (9 μΐ, 0.1 mmol) and aBH₃CN (14 mg, 0.2 mmol) in MeOH (1 mL) was stirred at room temperature overnight. The mixture was concentrated under vacuum. The residue was dissolved in DCM and then washed with water. The organic layer was separated and concentrated under vacuum. To the residue was added a solution of HCl in EtOAc (4 N). The mixture was stirred at room temperature for an hour. The mixture was concentrated under vacuum to dryness to give the title compound. MS-ESI (m/z): 337 [M+l]⁺.

EXAMPLE 6

l-((7_tS',8i?V7-amino-8-(2.4.5-trifluorophenylV3.4.6.7.8.9-hexahvdrobenzor4.51imidazori .2- alpyrazin-2(lH)-yl)ethanone bis-hydrochloric acid salt

[0194] A solution of ri-butyl ((75,8R)-8-(2,4,5-trifluorophenyl)-l ,2,3,4,6,7,8,9- octahydrobenzo[4,5]imidazo[l ,2-a]pyrazin-7-yl)carbamate (from EXAMPLE 3, Step B, 21 mg, 0.05 mmol), Et₃N (20 mg, 0.2 mmol), and Ac₂0 (10 mg, 0.1 mmol) in DCM (1 mL) was stirred at room temperature overnight. The mixture was washed with water and then concentrated under vacuum. To the residue was added a solution of HC1 in EtOAc (4 N). The mixture was stirred at room temperature for an hour. The mixture was concentrated under vacuum to dryness to give the title compound. 1H NMR (400 MHz, D₂0): δ 7.33 (m, 1H), 7.20 (m, 1H), 4.96 (s, 1H), 4.18 (s, 1H), 4.07 (m, 4H), 3.65 (m, 1H), 3.28 (d, 1H), 3.09 (m, 2H), 2.89 (t, 1H), 2.21 (d, 3H), 2.03 (s, 1H); MS-ESI (m z): 365 [M+l]⁺.

EXAMPLE 7

(75.8RV2-(methylsidfonvn-8-(2.4.5-trifluorophenvn-1.2.3.4.6.7.8.9- octahydrobenzo[4,51imidazo[l ,2-alpyrazin-7-amine bis-hydrochloric acid salt

[0195] To a solution of ierf-butyl ((75,8R)-8-(2,4,5-trifluorophenyl)-l,2,3,4,6,7,8,9- octahydrobenzo[4,5]imidazo[l ,2-a]pyrazin-7-yl)carbamate (from EXAMPLE 3, Step B, 50 mg, 0.1 mmol) and Et₃N (20 mg) in DCM (1 mL) was added MeS0₂Cl (22 mg, 0.05 mmol) at 0 ^°C. The mixture was stirred at room temperature for 2 hr. The mixture was washed with NaHC0₃ aq. soln. and concentrated under vacuum. The residue was purified by column chromatography, and then a solution of HC1 in EtOAc (4 N) was added. The mixture was stirred at room temperature for four hours. The mixture was concentrated under vacuum to dryness to give the title compound. ^!H NMR (400 MHz, D₂0): δ 7.34 (m, 1H), 7.20 (m, 1H), 4.20 (br, 2H), 4.10 (br, 1H), 3.87 (br, 2H), 3.61 (m, 2H), 3.28 (m, 1H), 3.09 (m, 6H), 2.89 (t, 1H). MS-ESI (m z): 401 [M+l]⁺.

EXAMPLE 8

(7_tS',8i?)-7-amino-8-(2,4,5-trifluoroDhenyl)-3,4,6,7,8,9-hexahvdrobenzo[4,51imidazo[l,2- alpyrazine-2(lH)-sulfonamide bis-hydrochloric acid salt

[0196] To a solution of imidazole (7 mg, O.lmmol) in DCM (1 mL) was added

NH₂S0₂C1 (6 mg, 0.05mmol) and then ierf-butyl ((75,8R)-8-(2,4,5-trifluorophenyl)- 1 ,2, 3,4,6,7, 8, 9-octahydrobenzo[4,5]imidazo[l ,2-a]pyrazin-7-yl)carbamate (from EXAMPLE 3, Step B, 5 mg, 0.01 mmol). The mixture was stirred at room temperature overnight. The mixture was washed with water and then concentrated under vacuum. To the residue was added a solution of HC1 in EtOAc (4 N). The mixture was stirred at room temperature for an hour. The mixture was concentrated under vacuum to dryness to give the title compound. ^!H NMR (400 MHz, D₂0): δ 7.34 (m, 1H), 7.20 (m, 1H), 4.68 (s, 2H), 4.21 (br, 2H), 4.10 (m, 1H), 3.76 (br, 2H), 3.65 (m, 1H), 3.28 (d, 1H), 3.09 (m, 2H), 2.92 (m, 1H); MS-ESI (m z): 402 [M+l]⁺.

EXAMPLE 9

(7_tS',8i?)-7-amino-8-(2,4,5-trifluorophenyl)-3,4,6,7,8,9-hexahydrobenzo[4,51imidazo[l,2- alpyrazine-2(lH)-carboxamide bis-hydrochloric acid salt

[0197] A mixture of tert-butyl ((75,8R)-8-(2,4,5-trifluorophenyl)-l,2,3,4,6,7,8,9- octahydrobenzo[4,5]imidazo[l ,2-a]pyrazin-7-yl)carbamate (from EXAMPLE 3, Step B, 21 mg, 0.05 mmol), KOCN (2 mg, 0.02 mmol), AcOH (0.2 mL), water (0.5 mL) in EtOH (0.5 mL) was stirred at room temperature overnight. To the mixture was added water, and then it was extracted with DCM. The extracts were concentrated under vacuum. To the residue was added a solution of HCl in EtOAc (4 N). The mixture was stirred at room temperature for an hour. The mixture was concentrated under vacuum to dryness to give the title compound. ^!H NMR (400 MHz, D₂0, ppm): δ 7.35 (m, 1H), 7.22 (m, 1H), 4.90 (s, 2H), 4.12 (br, 3H), 3.93 (br, 2H), 3.66 (m, 1H), 3.28 (d, 1H), 3.10 (m, 2H), 2.91 (t, 1H); MS-ESI (m/z): 366 [M+l]⁺.

EXAMPLE 10

l-((7_tS',8i?V7-amino-8-(2.4.5-trifluorophenylV3.4.6.7.8.9-hexahvdrobenzor4.51imidazori.2- alpyrimidin- 1 (2H)-yl)ethanone bis-hydrochloric acid salt

Step A. fe -Butyl ((75.8RV8-(2.4.5-trifluorophenvn- 1.2.3.4.6.7.8.9- octahydrobenzo[4,51imidazo[l,2-alpyrimidin-7-yl)carbamate

[0198] This compound was prepared by following Steps A to D in Example 1, substituting 2-amino-pyrazine with 2-amino-pyrimidine, and also following Steps A and B in Example 3. MS-ESI (m z): 419 [M+l]⁺.

Step B. l-((7_ty,8i?)-7-amino-8-(2.4.5-trifluorophenyl)-3.4.6.7.8.9- hexahydrobenzo[4,51imidazo[ 1 ,2-alpyrimidin- 1 (2H)-yl)ethanone [0199] This compound was prepared by following the procedure in Example 6, replacing ieri-butyl ((7»S',Sii)-8-(2,4,5-trifluorophenyl)- 1,2,3,4,6,7, 8,9-octahydrobenzo[4,5]imidazo[ 1 ,2- a]pyrazin-7-yl)carbamate (from EXAMPLE 3, Step B) with fe -butyl ((7_tS^,,8i?V8-(2.4.5- trifluorophenyl)-l, 2,3,4,6,7, 8,9-octahydrobenzo[4,51imidazo[l ,2-alpyrimidin-7-yl)carbamate (from Step A above). MS-ESI (m/z): 365 [M+l]⁺.

EXAMPLE 11

(7_tS',8i?Vl-methyl-8-(2.4.5-trifluorophenylV1.2.3.4.6.7.8.9-octahvdrobenzor4.51imidazori .2- alpyrimidin-7-amine bis-hydrochloric acid salt

[0200] This compound was prepared by following the procedure in Example 5 by replacing tert-butyl {{7S, 8i?)-8-(2,4,5-trifluorophenyl)- 1,2,3,4,6,7,8,9- octahydrobenzo[4,5]imidazo[l ,2-a]pyrazin-7-yl)carbamate (from Example 3, Step B) with tert- butyl ((7_tS',8^V8-(2.4.5-trifluorophenylV1.2.3.4.6.7.8.9-octahvdrobenzor4.51imidazori.2- alpyrimidin-7-vncarbamate (from Example 10, Step A). MS-ESI (m/z): 337 [M+l]⁺.

EXAMPLE 12

(7_tS',8^)-methyl 7-amino-8-(2.4.5-trifluorophenyl)-3.4.6.7.8.9- hexahvdrobenzo[4,51imidazo[l,2-alDyrimidine-l(2H)-carboxylate bis-hydrochloric acid salt

[0201] This compound was prepared by following the procedures in Example 3, Steps C and D, by replacing fe -butyl ((7£8ifl-8-(2A5-trifluorophenyl)-1.23A6.7.8.9- octahydrobenzo[4,51imidazo[ 1 ,2-alpyrazin-7-yl)carbamate (from Example 3, Step B) with tert- butyl ((7_tS',8^)-8-(2.4.5-trifluorophenyl)-1.2.3.4.6.7.8.9-octahvdrobenzor4.51imidazori.2- alpyrimidin-7-yl)carbamate (Example 10, Step A). MS-ESI (m/z): 381 [M+l]⁺.

EXAMPLE 13

(7_tS',8^)-8-(2.4.5-trifluorophenyl)-1.2.3.4.6.7.8.9-octahvdrobenzor4.51imidazori .2- alpyrimidin-7-amine

Step A. (7_tS',8^)-8-(2.4.5-trifluorophenyl)-1.2.3.4.6.7.8.9-octahvdrobenzor4.51imidazori .2- alpyrimidin-7-amine [0202] This compound was prepared by substituting 2-amino-pyrazine with 2-amino- pyrimidine in Example 1, following steps A to D.

Step B. (7_tS^,,8i?V8-(2.4.5-trifluorophenylV1.2.3.4.6.7.8.9-octahvdrobenzor4.51imidazori.2- alpyrimidin-7-amine

[0203] A suspension of (75,8R)-8-(2,4,5-trifluorophenyl)-l,2,3,4,6,7,8,9- octahydrobenzo[4,5]imidazo[l ,2-a]pyrimidin-7-amine (from Step A, 1 g, 3.14 mmol), 10% Pd/C (50mg) , and HCO₂NH₄ ( 1.98 g, 31.4 mmol ) in MeOH was stirred at room temperature overnight. The mixture was filtered, and the filtrate was concentrated under vacuum. To the residue of concentrate was added water, and then it was extracted with EtOAc. The extracts were washed with water, dried over anhydrous Na₂SC>4, and then concentrated under vacuum to give 760 mg the title compound as a yellowish white foam. MS-ESI (m/z): 323 [M+l]⁺.

DPP-4 activity in vitro

[0204] DPP-IV Assay Solution of test compounds in varying concentrations (10^~5 mol/L, 10^"6 mol/L, 10^"7 mol/L, 10^"8 mol/L, 10^"9 mol/L, 10^"10 mol/L, 10^"11 mol/L, and 10^"12 mol L) were prepared in dimethyl sulfoxide (DMSO) and then diluted into assay buffer comprising: 10 mM Tris-HCl pH 8.0, 0.2 M NaCl, and 0.1% BSA. Recombinant human DPP-IV (7.8 ng/ml final concentration) was added to the dilutions and pre-incubated for 30 min at room temperature before the reaction was initiated with H-Ala-Pro-AFC (50 μΜ final concentration). The total volume of the reaction mixture was 100 ul.

[0205] The fluorescence of the mixture was measured after 30 minutes (excitation at 405 nm; emission at 535 nm). Inhibition constants (IC₅₀) were calculated by GraphPad Prism.

[0206] The samples were tested according to the above-described assays for protease inhibition , the results are listed in Table 1.

Table 1

a]pyrazin-7-amine

(7i?,8S)-7-(2,4,5-trifluorophenyl)- 1 ,2,3,4,6,7,8,9-

4.3 octahydrobenzo[4,5]imidazo[l,2- a]pyridin-8-amine

(7S,8R)-met yl 7-amino-8-(2,4,5-

0 trifluorophenyl)-3,4,6,7,8,9-

MeO^N^N^^NH^HCI

hexahydr obenzo [4,5] imidazo [1,2- 1.0 a]pyrazine-2(lH)-carboxylate bis- hydrochloric acid salt

(7S,8i?)-methyl 7-amino-8-(2,5-

0 difluorophenyl)-3,4,6,7,8,9- hexahydr obenzo [4,5] imidazo [1,2- 23 a]pyrazine-2(lH)-carboxylate bis- hydrochloric acid salt

(7S,8tf)-2-methyl-8-(2,4,5- - 2HCI trifluorophenyl)- 1 ,2,3,4,6,7,8,9- octahydrobenzo[4,5]imidazo[l,2- 1.5 a]pyrazin-7-amine bis-hydrochloric acid salt

l-((7S,8i?)-7-amino-8-(2,4,5-

0 trifluorophenyl)-3,4,6,7,8,9- 2HCI

hexahydr obenzo [4,5] imidazo [1,2- 1.0 a]pyrazin-2(lH)-yl)ethanone bis- hydrochloric acid salt

(7S,8i?)-2-(methylsulfonyl)-8-(2,4,5- trifluorophenyl)- 1 ,2,3,4,6,7,8,9- 3.9

octahydrobenzo[4,5]imidazo[l,2-

Claims

WHAT IS CLAIMED IS:

1. At least one compound of formula (I):

and/or at least one pharmaceutically acceptable salt thereof, wherein

Ar is aryl unsubstituted or substituted with one to five R substituents;

each R is independently selected from:

halogen,

cyano,

hydroxy,

Ci-6 alkyl, wherein alkyl is unsubstituted or substituted with one to five halogens, and Ci-6 alkoxy, wherein alkoxy is unsubstituted or substituted with one to five halogens;

X and Y are each independently selected from CR 2 R 3 , and NR 4 ;;

R 2" and R 3^J are each independently selected from:

hydrogen,

halogen,

hydroxy,

Ci_6 alkyl, wherein alkyl is unsubstituted or substituted with one to five halogens, and Ci_6 alkoxy, wherein alkoxy is unsubstituted or substituted with one to five halogens; independently selected from:

hydrogen,

Ci-6 alkyl, wherein alkyl is unsubstituted or substituted with one to five substituents independently selected from halogen and hydroxy,

(CH₂)_n-aryl, wherein aryl is unsubstituted or substituted with one to five substituents independently selected from hydroxy, halogen, cyano, C0₂H, Ci-₆ alkyloxycarbonyl, Ci-₆ alkyl, and Ci-₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens, (CH₂)_n-heteroaryl, wherein heteroaryl is unsubstituted or substituted with one to three substituents independently selected from hydroxy, halogen, cyano, C0₂H, Ci_₆ alkyloxycarbonyl, Ci_₆ alkyl, and Ci_₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens,

(CH₂)_n-heterocyclyl, wherein heterocyclyl is unsubstituted or substituted with one to three substituents independently selected from oxo, hydroxy, halogen, cyano, C0₂H, C_1-6 alkyloxycarbonyl, Ci_₆ alkyl, and Ci_₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens,

(CH₂)_n-C₃_6 cycloalkyl, wherein cycloalkyl is unsubstituted or substituted with one to three substituents independently selected from halogen, hydroxy, cyano, C0₂H, C_1-6 alkyloxycarbonyl, C_1-6 alkyl, and C_1-6 alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens,

(CH₂)_n- -COCi-6 alkyl,

(CH₂)_n- -COCi-6 cycloalkyl,

(CH₂)„- -C0₂Ci_6 alkyl,

(CH₂)_n. -C0₂Ci_6 cycloalkyl,

(CH₂)„- -CONR⁵R⁶,

(CH₂)_n. -S(0)_mR⁶, and

(CH₂)„- -S0₂NR⁵R⁶,

wherein any individual methylene (CH₂) carbon atom in (CH₂)_n is unsubstituted or substituted with one to two groups independently selected from halogen, hydroxy, Ci_₆ alkyl, and Ci_₆alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens;

and R⁶ are each independently selected from

hydrogen,

(CH₂)„-aryl,

(CH₂)_n-C₃_6 cycloalkyl, and

Ci_6 alkyl,

further wherein aryl and cycloalkyl are each independently unsubstituted or substituted with one to five substituents independently selected from halogen, hydroxy, Ci_₆ alkyl, and Ci_6 alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens; or

R⁵ and R⁶ together with the nitrogen atom to which they are attached form a heterocyclic ring selected from azetidine, pyrrolidine, piperidine, piperazine, and morpholine, wherein said heterocyclic ring is unsubstituted or substituted with one to three substituents independently selected from halogen, hydroxy, Ci_₆ alkyl, and Ci_₆ alkoxy, wherein alkyl and alkoxy are each independently unsubstituted or substituted with one to five halogens;

m is selected from 0, 1 and 2; and

n is selected from 0, 1, 2, and 3.

2. At least one compound of claim 1, and/or at least one pharmaceutically acceptable salt thereof, wherein Ar is phenyl substituted with one to five R¹ substituents.

3. At least one compound of any one of claims 1 to 2, and/or at least one pharmaceutically acceptable salt thereof, wherein R¹ is halogen.

4. At least one compound of any one of claims 1 to 3, and/or at least one pharmaceutically acceptable salt thereof, wherein Ar is selected from 2,4,5-trifluorophenyl and 2,5-difluorophenyl.

5. At least one compound of any one of claims 1 to 4, and/or at least one pharmaceutically acceptable salt thereof, wherein Ar is 2,4,5-trifluorophenyl.

6. At least one compound of any one of claims 1 to 4, and/or at least one pharmaceutically acceptable salt thereof, wherein Ar is 2,5-difluorophenyl.

7. At least one compound of any one of claims 1 to 6, and/or at least one pharmaceutically acceptable salt thereof, wherein X is CR 2 R 3 and Y is CR 2 R 3.

8. At least one compound of any one of claims 1 to 6, and/or at least one pharmaceutically acceptable salt thereof, wherein X is NR 4 and Y is CR 2 R 3.

9. At least one compound of any one of claims 1 to 6, and/or at least one pharmaceutically acceptable salt thereof, wherein X is CR 2 R 3 and Y is NR 4.

10. At least one compound of any one of claims 1 to 9, and/or at least one pharmaceutically acceptable salt thereof, wherein R 2 and R 3 are hydrogen.

11. At least one compound of any one of claims 1 to 10, and/or at least one pharmaceutically acceptable salt thereof, wherein R⁴ is selected from hydrogen, C_1-6 alkyl , (CH2)_n-COCi_6 alkyl, (CH₂)n-C0₂Ci_₆ arkyl, (CH₂)_n-CONR⁵R⁶, (CH₂)_n-S(0)_mR⁶, and (CH₂)_n-S0₂NR⁵R⁶, wherein n is 0 and m is 2.

12. At least one compound of any one of claims 1 to 11, and/or at least one pharmaceutically acceptable salt thereof, wherein R⁴ is selected from H, CH₃, COCH₃, C0₂CH₃, CONH₂, S0₂CH₃, and S0₂NH₂.

13. At least one compound of any one of claims 1-5, 8, and 10-12, and/or at least one pharmaceutically acceptable salt thereof, wherein X is NR 4 and Y is CR 2 R 3 , Ar is 2,4,5- trifluorophenyl, wherein R 2 and R 3 are hydrogen, and R 4 is selected from H, CH₃, COCH₃, C0₂CH₃, CONH₂, S0₂CH₃, and S0₂NH₂.

14. At least one compound, selected from:

(7S, 8 i )-8-(2,4,5-trifluorophenyl)-l, 2,3,4,6,7, 8,9-octahydrobenzo[4,5]imidazo[l,2-a]pyrazin-

7- amine;

(7ii,8^)-7-(2,4,5-trifluorophenyl)-l, 2,3,4,6,7, 8, 9-octahydrobenzo[4,5]imidazo[l,2-a]pyridin-

8- amine;

(7, ,Sii)-methyl 7-amino-8-(2,4,5-trifluorophenyl)-3,4,6,7,8,9- hexahydrobenzo[4,5]imidazo[l,2-a]pyrazine-2(lH)-carboxylate;

(75,8R)-methyl 7-amino-8-(2,5-difluorophenyl)-3,4,6,7,8,9- hexahydrobenzo[4,5]imidazo[l,2-a]pyrazine-2(lH)-carboxylate; (7^,Sii)-2-methyl-8-(2,4,5 rifluorophenyl) ,2,3,4,6,7,8,9-octahydrobenzo[4,5]imidazo[l,2- a]pyrazin-7-amine;

l-((7^,Sii)-7-amino-8-(2,4,5-trifluorophenyl)-3,4,6,7,8,9-hexahydrobenzo[4,5]imidazo[l ,2- a]pyrazin-2(lH)-yl)ethanone;

(75, 8R)-2-(methylsulfonyl)-8-(2,4,5-trifluorophenyl)-l ,2,3,4,6,7,8,9- octahydrobenzo [4, 5 ] imidazo [1 ,2- a]pyr azin-7 -amine ;

(75,Sii)-7-amino-8-(2,4,5-trifluorophenyl)-3,4,6,7,8,9-hexahydrobenzo[4,5]imidazo[l,2- a]pyrazine-2(lH)-sulfonamide;

l-((75,Sii)-7-amino-8-(2,4,5-trifluorophenyl)-3,4,6,7,8,9-hexahydrobenzo[4,5]imidazo[l ,2- a]pyrimidin- 1 (2H)-yl)ethanone;

(75,Sii)-l-methyl-8-(2,4,5-trifluorophenyl)-l,2,3,4,6,7,8,9-octahydrobenzo[4,5]imidazo[l,2- a]pyrimidin-7-amine;

(75,8R)-methyl 7-amino-8-(2,4,5-trifluorophenyl)-3,4,6,7,8,9- hexahydrobenzo[4,5]imidazo[l,2-a]pyrimidine-l(2H)-carboxylate; and

(75',S^)-8-(2,4,5-trifluorophenyl) ,2,3,4,6,7,8,9-octahydrobenzo[4,5]irrddazo[l,2- a]pyrimidin-7-amine; and/or

at least one pharmaceutically acceptable salt thereof.

15. A pharmaceutical composition which comprises at least one compound of any one of claims 1 to 14, and/or at least one pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable carrier.

16. A method of treating a condition responsive to inhibition of dipeptidyl peptidase- IV enzyme comprising administering to a patient in recognized need thereof an effective amount of at least one compound of any one of claims 1 to 14, and/or at least one pharmaceutically acceptable salt thereof.

17. A method for treating a condition selected from insulin resistance, hyperglycemia, and type II diabetes comprising administering to a patient in recognized need thereof an effective amount of at least one compound of any one of claims 1 to 14, and/or at least one pharmaceutically acceptable salt thereof.