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WO2012068236A2 - Nouvelles oxydoréductases fongiques - Google Patents

Nouvelles oxydoréductases fongiques Download PDF

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Publication number
WO2012068236A2
WO2012068236A2 PCT/US2011/060970 US2011060970W WO2012068236A2 WO 2012068236 A2 WO2012068236 A2 WO 2012068236A2 US 2011060970 W US2011060970 W US 2011060970W WO 2012068236 A2 WO2012068236 A2 WO 2012068236A2
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Prior art keywords
seq
acid sequence
enzyme
amino acid
activity
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Ceased
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WO2012068236A3 (fr
Inventor
Johannes Visser
Sandra Hinz
Jan Werij
Jacob Visser
Vivi Joosten
Martijn Koetsier
Mark Emalfarb
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Dyadic International USA Inc
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Dyadic International USA Inc
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Publication of WO2012068236A3 publication Critical patent/WO2012068236A3/fr
Anticipated expiration legal-status Critical
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • This invention relates to novel enzymes and novel methods for producing the same.
  • Oxidoreductases represent a category of various enzymes including but not limited to oxidases, monooxygenases, Baeyer- Villiger monooxygenases, hydroxylases, peroxidases, dioxygenases, dehydrogenases, and reductases that catalyze an oxidation-reduction reaction.
  • the invention also relates to a method to aid in the conversion of plant biomass to biofuels by degrading lignin to allow enzymes used in the processing of plant biomass to more easily access the cellulose and hemicellulose and to novel combinations of enzymes, including those that provide a combined or synergistic release of sugars from plant biomass.
  • the invention also relates to methods of using the novel enzymes and compositions of such enzymes in a variety of other processes, such as bleaching pulp, cotton and flour; disinfecting food; preserving and increasing shelf life of food and beverages improving baking products; producing sugar alcohols; synthesizing amino acids, steroids, and other fine chemicals; manufacturing of products; and controlling pollution.
  • Oxidoreductases are enzymes that catalyze the transfer of electrons from one molecule (the reductant or electron donor) to another (the oxidant or electron acceptor).
  • Oxidoreductases can be oxidases or dehydrogenases.
  • Oxidases are enzymes that utilize molecular oxygen as an electron acceptor
  • dehydrogenases are enzymes that oxidize a substrate by transferring a hydride to an acceptor that is either an NAD /NADP + or a flavin-dependent enzyme.
  • Other oxidoreductases include peroxidases, oxygenases, hydroxylases, and reductases. Peroxidases catalyze the reduction of a peroxide..
  • Oxygenases catalyze the incorporation of oxygen from molecular oxygen into organic substrates.
  • Hydroxylases are a class of oxygenases that catalyze the introduction of hydroxyl groups to organic substrates.
  • Monooxygenases are a class of oxygenases that catalyze the incorporation of one atom of oxygen from molecular oxygen into organic substrates.
  • Dioxygenases are a class of oxygenases that catalyze the incorporation of both atoms of oxygen from molecular oxygen into organic substrates.
  • Baeyer-Villiger monooxygenases are a class of oxygenases that catalyze the insertion of an oxygen atom from molecular oxygen in a ketone, next to the carbonyl carbon atom.
  • Reductases catalyze reductions, in most cases reductases can act like an oxidase. Many pathways of biogenesis and bio degradation require oxidoreductase (dehydrogenase or reductase) activity, coupled to the reduction or oxidation of a donor or acceptor cofactor.
  • oxidoreductase dehydrogenase or reductase
  • Potential cofactors include cytochromes, oxygen, disulfide, iron-sulfur proteins, flavin adenine dinucleotide (FAD), and the nicotinamide adenine dinucleotides NAD and NADP (Newsholme, E. A. and Leech, A. R. (1983) Biochemistry for the Medical Sciences, John Wiley and Sons, Chichester, U. K. pp. 779-793).
  • Oxidoreductases have found a great number of uses in industry such as aiding in the production of bio fuels; bleaching pulp, cotton and flour; cleansing of food such as vegetables; improving the structure of baked goods; clarification of beer or wine; increasing shelf life of food and beverages; producing xylitol and other sugar alcohols such as sorbitol; synthesizing amino acids, steroids, and other fine chemicals; acting as biosensors; manufacturing adhesives, computer chips, and other products; controlling pollution and soil detoxification; whitening skin/hair, and teeth; various bioremediative processes and textile applications, such as denim treatment, stain removal, treatment of various fibers for textile industry, methods for decolorizing dyes and methods for treating dye house effluents, or use in hair dyeing composition, in hard-surface cleaning or in detergent formulations; and stimulating the immune system to name just a few applications.
  • Oxidoreductases such as laccases
  • have many industrially potential applications such as delignification of wood pulps, methods for treating lignin containing fibers, methods for treating wood fibers in order to functionalize them, improving of the production of fuel ethanol from renewable raw materials.
  • Plant biomass is being touted as the up-and-coming source for biofuels and chemical building blocks
  • biomass such as corn stover, switchgrass and other feedstocks
  • Lignin is a complex chemical compound that is an integral part of the secondary cell walls of many plants and some algae.
  • Lignin acts like a kind of glue holding together cellulose and hemicelluose, which are important ingredients in making ethanol or chemical building blocks
  • Oxidoreductases can act upon lignin or as reductases in the degradation of (hemi-) cellulose to allow the enzymes used in the processing of plant biomass to more easily access the cellulose and hemicellulose to create biofuels such as ethanol or chemical intermediates such as succinic acid.
  • biofuels such as ethanol or chemical intermediates such as succinic acid.
  • Pulp manufactured by the kraft process must be bleached to provide the desired whiteness of fine paper, tissue, and similar products. Traditionally, this was achieved using reactions involving chlorine and sodium hydroxide. More recently, chlorine dioxide has been increasingly substituted for chlorine. Due to environmental concerns over chloroorganic compounds present in the bleaching effluents, non-chlorine bleaching reactions are currently being introduced. These reactions include oxygen delignification followed by chelation and hydrogen peroxide oxidation, and combinations of ozone and other non-chlorine compounds. These processes are presently more costly and less selective for lignin than chlorine bleaching. Thus, there exists the need for more efficient oxidoreductase enzymes to create a more cost effective and environmentally friendly process for whitening paper and tissue products, and the novel enzymes of the present invention fulfil such a need.
  • the "stone washed" look has been used by the denim industry to produce a product that appears faded and worn. Because traditional stone washing with pumice stones reduces the strength of fabric and burdens the laundering apparatuses, the industry has turned towards an enzymatic denim finishing processes.
  • a "bleached look" of denim is normally obtained by means of bleaching chemicals, e.g. sodium hypochlorite.
  • bleaching chemicals e.g. sodium hypochlorite.
  • the hypochlorite process is environmentally very harmful, damages the fabric easily, and is possibly harmful for the user. Furthermore, it cannot be used for Lycra containing products, and antichlor treatment with several rinsing/washing steps is required.
  • oxidoreductases that are able to function at low temperatures to accommodate the newer dyes and finishes that must be washed at a lower temperature. Therefore, there exists a need for developing an ecologically less harmful alternative for sodium hypochlorite that can function at lower temperatures, in particular the oxidoreductases of the present invention, (e.g., laccases), can serve that purpose.
  • the oxidoreductases of the present invention e.g., laccases
  • Oxidoreductases are useful in the production of cheese.
  • Lactobionic acid can be used as the sole acidulent for direct acidification of cheese, or in conjunction with reduced amounts of lactic acid culture.
  • a lactose oxidase is used in one embodiment to convert lactose intrinsically present in the dairy liquid ingredient(s) into lactobionic acid. It is desirable to manufacture cheeses with reduced lactose content while preserving flavor, texture, and appearance comparable with conventional cheese products.
  • the present invention provides such enzymes to aid in the production of lactobionic acid.
  • Flavor problems caused by non-specific oxidants due to oxidation of other components of a food system can be eliminated due to the specificity of an enzyme catalyzed reaction.
  • One example of such a use includes a process in which treatment with sulfhydryl oxidase aids in the removal of a burnt flavor from Ultra- High Temperature (UHT) sterilized milk.
  • UHT Ultra- High Temperature
  • Filamentous fungi are a rich source of oxidoreductases, such as but not limited to oxidases, monooxygenases, Baeyer-Villiger monooxygenases, hydroxylases, peroxidases, dioxygenases, dehydrogenases, and reductases that catalyze an oxidation-reduction reaction. It is desirable to produce inexpensive enzymes and enzyme mixtures that efficiently aid in the conversion of (plant) biomass for use in a variety of industrial applications.
  • the present invention comprises an isolated nucleic acid sequence selected from the group consisting of:
  • nucleic acid sequence encoding an amino acid sequence that is at least about 70% identical to an amino acid sequence of (a) and has a biological activity of the protein comprising the amino acid sequence.
  • the nucleic acid sequence encodes an amino acid sequence that is at least about 90%, at least about 95%, at least about 97% or at least about 99% identical to the amino acid sequence of (a) and has a biological activity of the protein comprising the amino acid sequence.
  • the nucleic acid sequence encodes a protein comprising an amino acid sequence selected from SEQ ID NO: 2, SEQ ID No: 4, SEQ ID No: 6, SEQ ID No: 8, SEQ ID No: 10, SEQ ID No: 12, SEQ ID No: 14, SEQ ID No: 16, SEQ ID No: 18, SEQ ID No: 20, SEQ ID No: 22, SEQ ID No: 24, SEQ ID No: 26, SEQ ID No: 28, SEQ ID No: 30, SEQ ID No: 32, SEQ ID No: 34, SEQ ID No: 36, SEQ ID No: 38, SEQ ID No: 40, SEQ ID No: 42, SEQ ID No: 44, SEQ ID No: 46, SEQ ID No: 48, SEQ ID No: 50, SEQ ID No: 52, SEQ ID No: 54, SEQ ID No: 56, SEQ ID No: 58, SEQ ID No: 60, SEQ ID No: 62, SEQ ID No: 64, SEQ ID No: 66, SEQ ID No: 68,
  • the nucleic acid sequence comprises a nucleic acid sequence selected from SEQ ID No: 1, SEQ ID No: 3, SEQ ID No: 5, SEQ ID No: 7, SEQ ID No: 9, SEQ ID No: 11, SEQ ID No: 13, SEQ ID No: 15, SEQ ID No: 17, SEQ ID No: 19, SEQ ID No: 21, SEQ ID No: 23, SEQ ID No: 25, SEQ ID No: 27, SEQ ID No: 29, SEQ ID No: 31, SEQ ID No: 33, SEQ ID No: 35, SEQ ID No: 37, SEQ ID No: 39, SEQ ID No: 41, SEQ ID No: 43, SEQ ID No: 45, SEQ ID No: 47, SEQ ID No: 49, SEQ ID No: 51, SEQ ID No: 53, SEQ ID No: 55, SEQ ID No: 56, SEQ ID No: 57, SEQ ID No: 59, SEQ ID No: 61, SEQ ID No: 63, SEQ ID No: 65, SEQ ID No: 67,
  • the present invention comprises nucleic acid sequences that are fully complementary to any of the nucleic acid sequences described above.
  • the present invention comprises an isolated protein comprising an amino acid sequence encoded by any of the nucleic acid molecules described above.
  • the present invention comprises an isolated fusion protein comprising an isolated protein of the present invention fused to a protein comprising an amino acid sequence that is heterologous to the isolated protein.
  • the present invention comprises a kit for degrading a plant biomass to comprising at least one isolated protein of the present invention.
  • the present invention comprises a bleach comprising at least one isolated protein of the present invention.
  • the present invention comprises a composition for the degradation of lignin comprising at least one isolated protein of the present invention.
  • the present invention comprises a recombinant nucleic acid molecule comprising an isolated nucleic acid molecule of the present invention, operatively linked to at least one expression control sequence.
  • the recombinant nucleic acid molecule comprises an expression vector.
  • the recombinant nucleic acid molecule comprises a targeting vector.
  • the present invention comprises an isolated host cell transfected with a nucleic acid molecule of the present invention.
  • the host cell is a fungus.
  • the host cell is a filamentous fungus.
  • the filamentous fungus is from a genus selected from the group consisting of: Chrysosporium, Thielavia, Talaromyces, Neurospora, Aureobasidium, Filibasidium, Piromyces, Corynascus, Cryptococcus, Acremonium, Tolypocladium, Scytalidium, Schizophyllum, Sporotrichum, Penicillium, Gibberella, Myceliophthora, Mucor, Aspergillus, Fusarium, Humicola, and Trichoderma, and anamorphs and teleomorphs thereof.
  • the host cell is a bacterium.
  • the present invention comprises an oligonucleotide consisting essentially of at least 12 consecutive nucleotides of a nucleic acid sequence selected from SEQ ID No: 1, SEQ ID No: 3, SEQ ID No: 5, SEQ ID No: 7, SEQ ID No: 9, SEQ ID No: 11, SEQ ID No: 13, SEQ ID No: 15, SEQ ID No: 17, SEQ ID No: 19, SEQ ID No: 21, SEQ ID No: 23, SEQ ID No: 25, SEQ ID No: 27, SEQ ID No: 29, SEQ ID No: 31, SEQ ID No: 33, SEQ ID No: 35, SEQ ID No: 37, SEQ ID No: 39, SEQ ID No: 41, SEQ ID No: 43, SEQ ID No: 45, SEQ ID No: 47, SEQ ID No: 49, SEQ ID No: 51, SEQ ID No: 53, SEQ ID No: 55, SEQ ID No: 56, SEQ ID No: 57, SEQ ID No: 59, SEQ ID No:
  • the present invention comprises a kit comprising at least one oligonucleotide of the present invention.
  • the present invention comprises methods for producing a protein of the present invention, comprising culturing a cell that has been transfected with a nucleic acid molecule comprising a nucleic acid sequence encoding the protein, and expressing the protein with the transfected cell. In some embodiments, the present invention further comprises recovering the protein from the cell or from a culture comprising the cell.
  • the present invention comprises a genetically modified organism comprising components suitable for degrading lignin, wherein the organism has been genetically modified to express at least one protein of the present invention.
  • the genetically modified organism is a plant, alga, fungus or bacterium.
  • the fungus is yeast, mushroom or filamentous fungus.
  • the filamentous fungus is from a genus selected from the group consisting of: Chrysosporium, Thielavia, Neurospora, Aureobasidium, Filibasidium, Piromyces, Corynascus, Cryptococcus, Acremonium, Tolypocladium, Scytalidium, Schizophyllum, Sporotrichum, Penicillium, Talaromyces, Gibberella, Myceliophthora, Mucor, Aspergillus, Fusarium, Humicola, and Trichoderma.
  • the filamentous fungus is selected from the group consisting of: Trichoderma reesei, Trichoderma harzanium, Myceliophthora thermophila, Aspergillus niger, Aspergillus oryzae, Aspergillus japonicus, Aspergillus niger Penicillium canescens, Penicillium solitum, Penicillium funiculosum, Talaromyces emersonii, Talaromyces flavus, Talaromyces emersonii and Myceliophthora thermophila.
  • the genetically modified organism has been genetically modified to express at least one additional enzyme.
  • the additional enzyme is an enzyme selected from the group consisting of: cellulase, glucosidase, xylanase, xylosidase, ligninase, glucuronidase, arabinofuranosidase, arabinase, arabinogalactanase, esterase, lipase, pectinase, glucomannanase, amylase, laminarinase, xyloglucanase, galactanase, galactosidase, glucoamylase, pectate and pectin lyase, chitosanases, exo-P-D-glucosaminidase, and cellobiose dehydrogenase.
  • the genetically modified organism is a plant.
  • the present invention comprises a recombinant enzyme isolated from a genetically modified microorganism of the present invention.
  • the recombinant enzyme has been subjected to a purification step.
  • the present invention comprises a crude fermentation product produced by culturing the cells from the genetically modified organism of the present invention, wherein the crude fermentation product contains at least one protein of the present invention.
  • the present invention comprises a multi-enzyme composition comprising enzymes produced by a genetically modified organism of the present invention , and recovered therefrom.
  • the present invention comprises a multi-enzyme composition comprising at least one protein of the present inventions, and at least one additional protein for degrading a biomass material or a fragment thereof that has biological activity.
  • the present invention comprises a multi-enzyme composition comprising at least one protein selected from SEQ ID No: 1892, SEQ ID No: 1894, SEQ ID No: 1896, SEQ ID No: 1898, SEQ ID No: 1900, SEQ ID No: 1902, SEQ ID No: 1904, SEQ ID No: 1906, SEQ ID No: 1908, SEQ ID No: 1910, SEQ ID No: 1912, SEQ ID No: 1914, SEQ ID No: 1916, SEQ ID No: 1918, SEQ ID No: 1920, SEQ ID No: 1922, SEQ ID No: 1924, SEQ ID No: 1926, SEQ ID No: 1928, SEQ ID No: 1930, SEQ ID No: 1932, SEQ ID No: 1934, SEQ ID No: 1936, SEQ ID No: 1938, SEQ ID No: 1940, SEQ ID No: 1942, SEQ ID No: 1944, SEQ ID No: 1946, SEQ ID No: 1948, SEQ ID No: 1950, SEQ ID No:
  • the present invention comprises a multi-enzyme composition comprising at least one protein selected from SEQ ID No: 1892, SEQ ID No: 1894, SEQ ID No: 1896, SEQ ID No: 1898, SEQ ID No: 1900, SEQ ID No: 1902, SEQ ID No: 1904, SEQ ID No: 1906, SEQ ID No: 1908, SEQ ID No: 1910, SEQ ID No: 1912, SEQ ID No: 1914, SEQ ID No: 1916, SEQ ID No: 1918, SEQ ID No: 1920, SEQ ID No: 1922, SEQ ID No: 1924, SEQ ID No: 1926, SEQ ID No: 1928, SEQ ID No: 1930, SEQ ID No: 1932, SEQ ID No: 1934, SEQ ID No: 1936, SEQ ID No: 1938, SEQ ID No: 1940, SEQ ID No: 1942, SEQ ID No: 1944, SEQ ID No: 1946, SEQ ID No: 1948, SEQ ID No: 1950, SEQ ID No:
  • the present invention comprises a multi-enzyme composition
  • a multi-enzyme composition comprising at least one protein selected from SEQ ID NO: 234, SEQ ID NO: 786, SEQ ID NO: 828, SEQ ID NO: 836, SEQ ID NO: 1000, SEQ ID NO: 1774, SEQ ID NO: 1884, SEQ ID NO: 1886, SEQ ID NO: 1888 and SEQ ID NO: 1990, and at least one additional protein for degrading a biomass material or a fragment thereof that has biological activity.
  • the present invention relates generally to proteins that play a role in reduction- oxidation reactions.
  • the present invention relates to enzymes isolated from a filamentous fungal strain denoted herein as CI (Accession No. VKM F- 3500-D), nucleic acids encoding the enzymes, and methods of producing and using the enzymes.
  • the invention also provides compositions that include at least one of the enzymes described herein for uses including, but not limited to, the degradation/modification of lignin or (hemi-) cellulose.
  • the invention stems, in part, from the discovery of a variety of novel oxidoreductases produced by the CI fungus that exhibit high activity toward plant biomass.
  • the present invention also provides methods and compositions for aiding in the conversion of plant biomass to fermentable sugars that can, in turn, be converted to useful products.
  • Such products may include, without limitation, metabolites, and bio fuels.
  • the methods include methods for degrading lignin and lignocellulosic material using enzyme mixtures to liberate sugars.
  • the compositions of the invention include enzyme combinations that break down lignin and ligno cellulose.
  • lignin or "lignen” refers to complex polymers, the chief noncarbohydrate constituent of wood, that binds to cellulose fibers and hardens and strengthens the cell walls of plants. Lignin is an integral part of the secondary cell walls of many plants and some algae. Lignin acts to hold together cellulose and hemicelluose, which are important ingredients in making ethanol.
  • biomass or "lignocellulosic material” includes materials containing cellulose and/or hemicellulose. Generally, these materials also contain pectin, lignin, protein, carbohydrates (such as starch and sugar) and ash. Ligno cellulose is generally found, for example, in the stems, leaves, hulls, husks, and cobs of plants or leaves, branches, and wood of trees.
  • Fermentable sugars refers to simple sugars, such as glucose, xylose, arabinose, galactose, mannose, rhamnose, sucrose and fructose.
  • Biomass can include virgin biomass and/or non-virgin biomass such as agricultural biomass, commercial organics, construction and demolition debris, municipal solid waste, waste paper and yard waste.
  • biomass include trees, shrubs and grasses, wheat, wheat straw, sugar cane bagasse, sugar beet, soybean, corn, corn husks, corn kernel including fiber from kernels, products and byproducts from milling of grains such as corn, tobacco, wheat and barley (including wet milling and dry milling) as well as municipal solid waste, waste paper and yard waste.
  • the biomass can also be, but is not limited to, herbaceous material, agricultural residues, forestry residues, municipal solid wastes, waste paper, and pulp and paper mill residues.
  • Agricultural biomass includes branches, bushes, canes, corn and corn husks, energy crops, algae, fruits, flowers, grains, grasses, herbaceous crops, leaves, bark, needles, logs, roots, saplings, short rotation woody crops, shrubs, switch grasses, trees, vegetables, fruit peels, vines, sugar beet pulp, wheat midlings, oat hulls, peat moss, mushroom compost and hard and soft woods (not including woods with deleterious materials).
  • agricultural biomass includes organic waste materials generated from agricultural processes including farming and forestry activities, specifically including forestry wood waste. Agricultural biomass may be any of the aforestated singularly or in any combination or mixture thereof.
  • Energy crops are fast-growing crops that are grown for the specific purpose of producing energy, including without limitation, biofuels, from all or part of the plant.
  • Energy crops can include crops that are grown (or are designed to grow) for their increased cellulose, xylose and sugar contents. Examples of such plants include, without limitation, switchgrass, willow and poplar.
  • Energy crops may also include algae, for example, designer algae that are genetically engineered for enhanced production of hydrogen, alcohols, and oils, which can be further processed into diesel and jet fuels, as well as other bio-based products.
  • biomass high in starch, sugar, or protein such as corn, grains, fruits and vegetables are usually consumed as food.
  • biomass high in cellulose, hemicellulose and lignin are not readily digestible and are primarily utilized for wood and paper products, animal feed, fuel, or are typically disposed.
  • the substrate is of high ligno cellulose content, including distillers' dried grains corn stover, corn cobs, rice straw, wheat straw, hay, sugarcane bagasse, sugar cane pulp, citrus peels and other agricultural biomass, switchgrass, forestry wastes, poplar wood chips, pine wood chips, sawdust, yard waste, and the like, including any combination thereof.
  • the present invention includes enzymes or compositions thereof with, for example, oxidoreductases, cellobiohydrolase, endoglucanase, xylanase, ⁇ -glucosidase, and hemicellulase activities.
  • Fermentable sugars can be converted to useful value-added fermentation products, non-limiting examples of which include amino acids, vitamins, pharmaceuticals, animal feed supplements, specialty chemicals, chemical feedstocks, plastics, solvents, fuels, or other organic polymers, lactic acid, and ethanol, including fuel ethanol.
  • Specific value-added products that may be produced by the methods of the invention include, but not limited to, biofuels (including ethanol and butanol); lactic acid; plastics; specialty chemicals; organic acids, including citric acid, succinic acid and maleic acid; solvents; animal feed supplements; pharmaceuticals; vitamins; amino acids, such as lysine, methionine, tryptophan, threonine, and aspartic acid; industrial enzymes, such as proteases, cellulases, amylases, glucanases, xylanases, arabinanases, lactases, lipases, esterases, lyases, oxidoreductases, transferases ; and chemical feedstocks.
  • the enzymes of the present invention may be used alone, or in combination with other enzymes, chemicals or biological materials.
  • the enzymes of the present invention may be used for in vitro applications in which the enzymes or mixtures thereof are added to or mixed with the appropriate substrates to catalyze the desired reactions. Additionally, the enzymes of the present invention may be used for in vivo applications in which nucleic acid molecules encoding the enzymes are introduced into cells and are expressed therein to produce the enzymes and catalyze the desired reactions within the cells.
  • enzymes capable of promoting cell wall degradation may be added to algal cells suspended in solutions to degrade the algal cell walls and release their content, whereas in some embodiments, nucleic acid molecules encoding such enzymes may be introduced into the algal cells to express the enzymes therein, so that these enzymes can degrade the algal cell walls from within.
  • Some embodiments may combine the in vitro applications with the in vivo applications. In some embodiments, the enzymes used for in vitro applications may be different from the enzymes used for in vivo applications.
  • the present invention includes proteins isolated from, or derived from the knowledge of enzymes from, a fungus such as Myceliophthora thermophila or a mutant or other derivative thereof, and more particularly, from the fungal strain denoted herein as CI (Accession No. VKM F-3500-D).
  • M. thermophila has previously appeared in patent applications and in the literature as Myceliophthora thermophila or Sporotrichum thermophile.
  • the proteins of the invention possess enzymatic activity.
  • U.S. Patent No. 6,015,707 or U.S. Patent No. 6,573,086 a strain called CI (Accession No.
  • VKM F-3500-D was isolated from samples of forest alkaline soil from Sola Lake, Far East of the Russian Federation. This strain was deposited at the All-Russian Collection of Microorganisms of Russian Academy of Sciences (VKM), Bakhurhina St. 8, Moscow, Russia, 113184, under the terms of the Budapest Treaty on the International Regulation of the Deposit of Microorganisms for the Purposes of Patent Procedure on August 29, 1996, as Myceliophthora thermophila Garg 27K, VKM-F 3500 D. Various mutant strains of M. thermophila (C. lucknowense) CI have been produced and these strains have also been deposited at the All-Russian Collection of Microorganisms of Russian Academy of Sciences (VKM), Bakhurhina St.
  • Strain CI was mutagenised by subjecting it to ultraviolet light to generate strain UV13-6 (Accession No. VKM F-3632 D). This strain was subsequently further mutated with N-methyl-N- nitro-N-nitrosoguanidine to generate strain NG7C-19 (Accession No. VKM F-3633 D).
  • strain UV18-25 (Accession No. VKM F-3631 D).
  • strain W1L (Accession No. CBS 122189), which was subsequently subjected to mutation by ultraviolet light, resulting in strain W1L#100L (Accession No. CBS122190).
  • Strain CI was initially classified as a Myceliophthora thermophila based on morphological and growth characteristics of the microorganism, as discussed in detail in U.S. Patent No. 6,015,707, U.S. Patent No. 6,573,086 and patent PCT/NL2010/000045. The CI strain was subsequently reclassified as M. thermophila based on genetic tests.
  • a protein of the invention comprises, consists essentially of, or consists of an amino acid sequence selected from SEQ ID NO: 2, SEQ ID No: 4, SEQ ID No: 6, SEQ ID No: 8, SEQ ID No: 10, SEQ ID No: 12, SEQ ID No: 14, SEQ ID No: 16, SEQ ID No: 18, SEQ ID No: 20, SEQ ID No: 22, SEQ ID No: 24, SEQ ID No: 26, SEQ ID No: 28, SEQ ID No: 30, SEQ ID No: 32, SEQ ID No: 34, SEQ ID No: 36, SEQ ID No: 38, SEQ ID No: 40, SEQ ID No: 42, SEQ ID No: 44, SEQ ID No: 46, SEQ ID No: 48, SEQ ID No: 50, SEQ ID No: 52, SEQ ID No: 54, SEQ ID No: 56, SEQ ID No: 58, SEQ ID No: 60, SEQ ID No: 62, SEQ ID No: 64, SEQ ID No: 66, SEQ ID
  • the present invention also includes homologues or variants of any of the above sequences, including fragments and sequences having a given identity to any of the above sequences, wherein the homologue, variant, or fragment has at least one biological activity of the wild-type protein, as described herein.
  • oxidoreductase refers to an enzyme that catalyzes the transfer of electrons from one molecule (the reductant, also called the hydride or electron donor) to another (the oxidant, also called the idem or electron acceptor).
  • reductant also called the hydride or electron donor
  • oxidant also called the idem or electron acceptor
  • Oxidase refers to any enzyme that catalyzes an oxidation-reduction reaction involving molecular oxygen (O 2 ) as the electron acceptor.
  • oxidoreductases are oxidoreductases that induce the incorporation of one atom of oxygen from 0 2 into the substance being oxidized.
  • Baeyer-Villiger Monooxygenases are oxidoreductases that induce the incorporation of one atom of oxygen from O 2 next to the carbonyl goup of the ketone substance being oxidized.
  • Peroxidases are enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e " ) + 2H + ⁇ ROH + R'OH.
  • Hydroxylases are oxidoreductases that induce the introduction of a hydroxyl group in the substance being oxidized.
  • Dioxygenases are enzymes that incorporate both atoms of O 2 into one substrate.
  • Dehydrogenases refer to enzymes that catalyze the removal of hydrogen from organic compounds.
  • Reductases refers to any enzyme that catalyzes the reduction of a substrate.
  • Cytochrome-c oxidase is an enzyme that receives an electron from each of four cytochrome c molecules, and transfers them to one oxygen molecule, converting molecular oxygen to two molecules of water.
  • Xanthine oxidoreductase is an enzyme that generates reactive oxygen species.
  • Xanthine oxidase is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid.
  • Redwood dehydrogenase refers to a protein that oxidizes cellobiose to cellobiono lactone .
  • Sulfhydryl oxidases or SOX are enzymes known to catalyze the conversion of thio compounds to the corresponding disulfides. Sulfhydryl oxidases are therefore of interest in applications where oxidation of free sulfhydryl groups to disulfide linkages is sought.
  • oxidases are those catalyzing the oxidation of sugars such as glucose oxidase, galactose oxidase and hexose oxidase .
  • Laccases belong to a family of multi-copper oxidases. Laccases are characterized by low substrate specificity, oxidizing various substrate, including diphenols, polyphenols, different substituted phenols, diamines, aromatic amines, and even inorganic compounds like iodine. Laccases oxidize their substrates by a one- electron oxidation mechanism, and they use molecular oxygen as an electron acceptor. Among laccases the primary sequence, induction mechanism, physico- chemical (e.g. isoelectric point and carbohydrate content) and biochemical characteristics are variable. The copper binding sites of laccases are, however, strictly conserved.
  • proteins disclosed herein possess oxidoreductase activity.
  • the proteins of the present invention can be combined with various enzymes as discussed below to aid in the production of bio fuels.
  • carbohydrase refers to any protein that catalyzes the hydrolysis of carbohydrates.
  • glycoside hydrolase refers to a protein that catalyzes the hydrolysis of the glycosidic bonds between carbohydrates or between a carbohydrate and a non-carbohydrate residue.
  • Endoglucanases cellobiohydrolases, ⁇ -glucosidases, a-glucosidases, xylanases, ⁇ - xylosidases, alpha- xylosidases, galactanases, a-galactosidases, ⁇ -galactosidases, a- amylases, glucoamylases, endo-arabinases, arabinofuranosidases, mannanases, ⁇ - mannosidases, pectinases, acetyl xylan esterases, acetyl mannan esterases, ferulic acid esterases, coumaric acid esterases, pectin methyl esterases, and chitosanases are examples of glycosidases.
  • Cellulose is a linear beta-(l-4) glucan consisting of anhydrocellobiose units.
  • Cellulases include endoglucanases, cellobiohydrolases, and ⁇ -glucosidases.
  • Cellulase refers to a protein that catalyzes the hydrolysis of l,4 ⁇ -D-glycosidic linkages in cellulose; cellulose derivatives (such as carboxymethylcellulose and hydroxyethylcellulose); plant lignocellulosic materials, beta-D-glucans or xyloglucans.
  • Endoglucanase refers to a protein that catalyzes the hydrolysis of cellulose to oligosaccharide chains at random locations by means of an endoglucanase activity.
  • Cellobiohydrolase refers to a protein that catalyzes the hydrolysis of cellulose to cellobiose via an exoglucanase activity, sequentially releasing molecules of cellobiose from the reducing or non-reducing ends of cellulose or cello - oligosaccharides.
  • ⁇ -glucosidase refers to an enzyme that catalyzes the conversion of cellobiose and oligosaccharides to glucose.
  • Hemicellulase refers to a protein that catalyzes the hydrolysis of hemicellulose, such as that found in lignocellulosic materials. Hemicelluloses are complex polymers, and their composition often varies widely from organism to organism, and from one tissue type to another. Hemicelluloses include a variety of compounds, such as xylans, arabinoxylans, xyloglucans, mannans, glucomannans, pectins, polygalacturonan, rhamnogalacturonan, xylogalacturonan and galacto(gluco)mannans. Hemicellulose can also contain glucan, which is a general term for beta-linked glucose residues.
  • a main component of hemicellulose is beta-l,4-linked xylose, a five carbon sugar.
  • this xylose is often branched as beta- 1,3 linkages or beta- 1,2 linkages, and can be substituted with linkages to arabinose, galactose, mannose, glucuronic acid, or by esterification to acetic acid.
  • hemicellulose is very different in dicotyledonous plants (dicots, i.e., plant whose seeds have two cotyledons or seed leaves such as lima beans, peanuts, almonds, peas, kidney beans) as compared to monocotyledonous plants (monocots; i.e., plants having a single cotyledon or seed leaf such as corn, wheat, rice, grasses, barley).
  • dicots i.e., plants having a single cotyledon or seed leaf such as corn, wheat, rice, grasses, barley.
  • hemicellulose is comprised mainly of xyloglucans that are 1,4- beta-linked glucose chains with 1,6-alpha-linked xylosyl side chains.
  • hetero xylans In monocots, including most grain crops, the principal components of hemicellulose are hetero xylans. These are primarily comprised of 1 ,4-beta- linked xylose backbone polymers with 1,2- or 1,3-alpha linkages to arabinose, linkage of galactose and mannose to arabinose or xylose in side chains, as well as xylose modified by ester- linked acetic acids. Also present are branched beta glucans comprised of 1,3- and 1 ,4-beta- linked glucosyl chains. In monocots, cellulose, hetero xylans and beta glucans are present in roughly equal amounts, each comprising about 15-25% of the dry matter of cell walls.
  • Hemicellulo lytic enzymes include both endo-acting and exo-acting enzymes, such as xylanases, ⁇ -xylosidases. alpha- xylosidases, galactanases, a-galactosidases, ⁇ -galactosidases, endo-arabinases, arabinofuranosidases, mannanases, ⁇ -mannosidases. Hemicellulases also include the accessory enzymes, such as acetylesterases, ferulic acid esterases, and coumaric acid esterases.
  • xylanases and acetyl xylan esterases cleave the xylan and acetyl side chains of xylan and the remaining xylo -oligomers are unsubstituted and can thus be hydro lysed with ⁇ -xylosidase only.
  • xylanases, acetylesterases and ⁇ -xylosidases are examples of hemicellulases.
  • Xylanase specifically refers to an enzyme that hydro lyzes the ⁇ -1,4 bond in the xylan backbone, producing short xylooligosaccharides.
  • ⁇ -Mannanase or "endo-l,4 ⁇ -mannosidase” refers to a protein that hydro lyzes mannan-based hemicelluloses (mannan, glucomannan, galacto(gluco)mannan) and produces short ⁇ -l,4-mannooligosaccharides.
  • Mannan endo-l,6-a-mannosidase refers to a protein that hydro lyzes 1,6-a- mannosidic linkages in unbranched 1,6-mannans.
  • ⁇ -Mannosidase ( ⁇ -l,4-mannoside mannohydrolase; EC 3.2.1.25) refers to a protein that catalyzes the removal of ⁇ -D-mannose residues from the nonreducing ends of oligosaccharides.
  • Galactanase refers to a protein that catalyzes the hydrolysis of ⁇ -1,4- ⁇ - ⁇ - galactosidic linkages in arabinogalactans.
  • Gluco amylase refers to a protein that catalyzes the hydrolysis of terminal 1,4- linked a-D-glucose residues successively from non-reducing ends of the glycosyl chains in starch with the release of ⁇ -D-glucose.
  • ⁇ -hexosaminidase or " ⁇ - ⁇ -acetylglucosaminidase” refers to a protein that catalyzes the hydrolysis of terminal N-acetyl-D-hexosamine residues in N-acetyl- ⁇ -
  • a-L-arabinofuranosidase refers to a protein that hydro lyzes arabinofuranosyl-containing hemicelluloses. Some of these enzymes remove arabino fur ano side residues from 0-2 or 0-3 single substituted xylose residues, as well as from 0-2 and/or 0-3 double substituted xylose residues.
  • Endo-arabinase refers to a protein that catalyzes the hydrolysis of 1,5-a- arabinofuranosidic linkages in 1,5-arabinans.
  • Exo-arabinase refers to a protein that catalyzes the hydrolysis of 1,5-a-linkages in 1,5-arabinans or 1,5-a-L arabino-oligosaccharides, releasing mainly arabinobiose, although a small amount of arabinotriose can also be liberated.
  • ⁇ -xylosidase refers to a protein that hydro lyzes short l,4-P-D-xylooligomers into xylose.
  • Cellobiose dehydrogenase refers to a protein that oxidizes cellobiose to cellobiono lactone .
  • Chitosanase refers to a protein that catalyzes the endohydro lysis of P-l,4-linkages between D-glucosamine residues in acetylated chitosan (i.e., deacetylated chitin).
  • Exo -polygalacturonase refers to a protein that catalyzes the hydrolysis of terminal alpha 1 ,4-linked galacturonic acid residues from non-reducing ends thus converting polygalacturonides to galacturonic acid.
  • Alcohol xylan esterase refers to a protein that catalyzes the removal of the acetyl groups from xylose residues.
  • Alcohol mannan esterase refers to a protein that catalyzes the removal of the acetyl groups from mannose residues
  • ferulic esterase or "ferulic acid esterase” refers to a protein that hydro lyzes the ester bond between the arabinose substituent group and ferulic acid.
  • Coumaric acid esterase refers to a protein that hydro lyzes the ester bond between the arabinose substituent group and coumaric acid.
  • Acetyl xylan esterases, ferulic acid esterases and pectin methyl esterases are examples of carbohydrate esterases.
  • Pectate lyase and pectin lyases refer to proteins that catalyze the cleavage of 1 ,4-a-D-galacturonan by beta-elimination acting on polymeric and/or oligosaccharide substrates (pectates and pectins, respectively).
  • Endo-l,3- -glucanase or “laminarinase” refers to a protein that catalyzes the cleavage of 1,3-linkages in ⁇ -D-glucans such as laminarin or lichenin.
  • Laminarin is a linear polysaccharide made up of ⁇ -1 -glucan with -l,6-linkages.
  • lichenan refers to a protein that catalyzes the hydrolysis of lichenan, a linear, 1,3-1,4- ⁇ - ⁇ glucan.
  • Rhamnogalacturonan is composed of alternating a-l,4-rhamnose and a-l,2-linked galacturonic acid, with side chains linked 1,4 to rhamnose.
  • the side chains include Type I galactan, which is -l,4-linked galactose with a-l,3-linked arabinose substituents; Type II galactan, which is -l,3-l,6-linked galactoses (very branched) with arabinose substituents; and arabinan, which is a-l,5-linked arabinose with a- 1,3-linked arabinose branches.
  • the galacturonic acid substituents may be acetylated and/or methylated.
  • "Exo-rhamnogalacturonanase” refers to a protein that catalyzes the degradation of the rhamnogalacturonan backbone of pectin from the nonreducing end.
  • Rhamnogalacturonan acetylesterase refers to a protein that catalyzes the removal of the acetyl groups ester-linked to the highly branched rhamnogalacturonan (hairy) regions of pectin.
  • Rhamnogalacturonan lyase refers to a protein that catalyzes the degradation of the rhamnogalacturonan backbone of pectin via a ⁇ -elimination mechanism (see, e.g., Pages et al, J. Bacteriol. 185:4727-4733 (2003)).
  • Alpha-rhamnosidase refers to a protein that catalyzes the hydrolysis of terminal non-reducing a-L-rhamnose residues in a-L-rhamno sides.
  • Glycosidases glycoside hydrolases; GH
  • GH glycoside hydrolases
  • Glycosidases such as the proteins of the present invention may be assigned to families on the basis of sequence similarities, and there are now over 100 different such families defined (see the CAZy (Carbohydrate Active EnZymes database) website, maintained by the Architecture of Fonction de Macromolecules Bi Anlagens of the Centre National de la Recherche Scientifique, which describes the families of structurally-related catalytic and carbohydrate- binding modules (or functional domains) of enzymes that degrade, modify, or create glycosidic bonds; Coutinho, P.M. & Henrissat, B. (1999) Carbohydrate-active enzymes: an integrated database approach. In “Recent Advances in Carbohydrate Bioengineering", H.J. Gilbert, G. Davies, B.
  • sequence homology may be used to identify particular domains within proteins, such as carbohydrate binding modules (CBMs; also known as carbohydrate binding domains (CBDs), sometimes called cellulose binding domains).
  • CBMs carbohydrate binding modules
  • CBDs carbohydrate binding domains
  • the CAZy homologies of proteins of the present invention are disclosed below.
  • An enzyme assigned to a particular CAZy family may exhibit one or more of the enzymatic activities or substrate specificities associated with the CAZy family. In other embodiments, the enzymes of the present invention may exhibit one or more of the enzyme activities discussed above.
  • Certain proteins used in the multi-enzyme compositions of the present invention may be classified as "Family 61 glycosidases" based on homology of the polypeptides to CAZy Family GH61.
  • Family 61 glycosidases may exhibit oxidative activity towards biopolymers including, but not limited to, cellulose, hemicellulose, chitin, chitosan, amylose, amylopectin, pectin and lignin.
  • the oxidative activity towards the biopolymers may results in an enhancing effect on the degradation of the corresponding biopolymer.
  • Polynuceo tides encoding "Family 61 glycosidases” and polypetides thereof are provided as SEQ ID NO: 1892 through SEQ ID NO: 1944. Additional information on the properties of Family 61 glycosidases may be found in U.S. Patent Application Publication Nos. 2005/0191736, 2006/0005279, 2007/0077630, and in PCT Publication No. WO 2004/031378.
  • Cellulo lytic enhancing activity refers to a biological activity that enhances the hydrolysis of a cellulo sic material by proteins having cellulo lytic activity. This enhancing activity is expected to be related by the cellulose oxidizing activity of the GH61 enzyme.
  • Hemicellulo lytic enhancing activity refers to a biological activity that enhances the hydrolysis of a hemicellulo sic material by proteins having hemicellulo lytic activity. This enhancing activity is expected to be related by the hemicellulose oxidizing activity of the GH61 enzyme.
  • hemicellulo stic materials include, but are not limited to, xylan, glucuronoxylan, arabinoxylan, glucomannan, and xyloglucan
  • Chitino lytic enhancing activity refers to a biological activity that enhances the hydrolysis of a chitinoic material by proteins having chitinase activity. This enhancing activity is expected to be related by the chitin oxidizing activity of the GH61 enzyme.
  • Amylo lytic enhancing activity refers to a biological activity that enhances the hydrolysis of a amylosic material by proteins having amylase activity. This enhancing activity is expected to be related by the amylose oxidizing activity of the GH61 enzyme.
  • Amylopectino lytic enhancing activity refers to a biological activity that enhances the hydrolysis of a amylopectinoic materials by proteins having amylopectinase activity. This enhancing activity is expected to be related by the amylopectin oxidizing activity of the GH61 enzyme.
  • Pectino lytic enhancing activity refers to a biological activity that enhances the hydrolysis of a pectinoic materials by proteins having pectinase activity. This enhancing activity is expected to be related by the pectin oxidizing activity of the GH61 enzyme.
  • “Lignino lytic enhancing activity” refers to a biological activity that enhances the hydrolysis of a ligno lytic materials by proteins having ligninase activity. This enhancing activity is expected to be related by the lignin oxidizing activity of the GH61 enzyme.
  • Cellobiose dehydrogenases and “cellobiose oxidases” are oxidoreductases that oxidize cellobiose to cellobiono-l,5-lactone and can utilize electron acceptors including, but not limited to, molecular oxygen, CDH-like reductases, GH61 enzymes, cytochrome c and Felll
  • CDH-like reductases are oxidoreductases that can be reduced by an oxidoreductase such as, but not limited to, Cellobiose dehydrogenase, and that can be oxidized by "CDH-like reductases” and GH61 enzymes.
  • the polynucelo tides encoding the CDH-like reductases and the polypeptides thereoff are provided as sequences SEQ ID NO: 234, SEQ ID NO: 1000, SEQ ID NO: 1774, SEQ ID NO: 1884, SEQ ID NO: 1886, SEQ ID NO: 1888 and SEQ ID NO: 1890.
  • This enhancing activity observed for the GH61 enzymes is expected to be related to the reduction of the GH61 enzyme by oxidoreductases such as, but not limited to, cellobiose dehydrogenases and CDH-like reductases.
  • Reduced GH61 in combination hydrogen peroxide can oxidize biopolymers.
  • the oxidation of biopolymers by the GH61 enzyme enhances the degradation of the biopolymer.
  • Proteins used in the multi-enzyme compositions of the present invention may also include homologues, variants, and fragments of the proteins disclosed herein.
  • the protein fragments include, but are not limited to, fragments comprising a catalytic domain (CD) and/or a carbohydrate binding module (CBM) (also known as a cellulose-binding domain; both can be referred to herein as CBM).
  • CD catalytic domain
  • CBM carbohydrate binding module
  • the identity and location of domains within proteins of the present invention are disclosed in detail below.
  • the present invention encompasses all combinations of the disclosed domains.
  • a protein fragment may comprise a CD of a protein but not a CBM of the protein or a CBM of a protein but not a CD.
  • domains from different proteins may be combined. Protein fragments comprising a CD, CBM or combinations thereof for each protein disclosed herein can be readily produced using standard techniques known in the art.
  • a protein fragment comprises a domain of a protein that has at least one biological activity of the full-length protein. Homologues or variants of proteins of the invention that have at least one biological activity of the full-length protein are described in detail below.
  • a protein fragment comprises a domain of a protein that has the catalytic activity of the full-length enzyme.
  • Oxidoreductases represent a category of various enzymes including but not limited to oxidases, oxygenases, monoxygenases, Baeyer-Villiger monooxygenases, dioxygenases, peroxidases, dehydrogenases, reductases that catalyze an oxidation- reduction reaction.
  • Sequence Oxid-1 scaffold00008.G4 is encoded by the nucleotides of SEQ ID No: 1 which encodes the amino acid sequence of SEQ ID NO: 2. This enzyme is believed to have cytochrome-c oxidase activity.
  • Sequence Oxid-2 scaflbld00016.G278 is encoded by the nucleotides of SEQ ID No: 3 which encodes the amino acid sequence of SEQ ID No: 4. This enzyme is believed to have cytochrome-c oxidase activity.
  • Sequence Oxid-3 scaffold00016.G284 is encoded by the nucleotides of SEQ ID No: 5 which encodes the amino acid sequence of SEQ ID No: 6. This enzyme is believed to have ent-kaurene oxidase activity.
  • Sequence Oxid-4 scaffold00016.G642 is encoded by the nucleotides of SEQ ID No: 7 which encodes the amino acid sequence of SEQ ID No: 8. This enzyme is believed to have cytochrome-c oxidase activity.
  • Sequence Oxid-5 scaffold00016.G1015 is encoded by the nucleotides of SEQ ID No: 9 which encodes the amino acid sequence of SEQ ID No: 10. This enzyme is believed to have coproporphyrinogen oxidase activity.
  • Sequence Oxid-6 scaffold00016.Gl 147 is encoded by the nucleotides of SEQ ID No: 11 which encodes the amino acid sequence of SEQ ID No: 12. This enzyme is believed to have xanthine oxidase activity.
  • Sequence Oxid-7 scaffold00031.G10 is encoded by the nucleotides of SEQ ID No: 13 which encodes the amino acid sequence of SEQ ID No: 14. This enzyme is believed to have catalase activity.
  • Sequence Oxid-8 scaffold00031.G81 is encoded by the nucleotides of SEQ ID No: 15 which encodes the amino acid sequence of SEQ ID No: 16. This enzyme is believed to have glutathione peroxidase activity.
  • Sequence Oxid-9 scaffold00031.G109 is encoded by the nucleotides of SEQ ID No: 17 which encodes the amino acid sequence of SEQ ID No: 18. This enzyme is believed to have cytochrome-c oxidase activity.
  • Sequence Oxid-10 scaffold00031.G306 is encoded by the nucleotides of SEQ ID No: 19 which encodes the amino acid sequence of SEQ ID No: 20. This enzyme is believed to have urate oxidase activity.
  • Sequence Oxid-11 scaffold00031.G894 is encoded by the nucleotides of SEQ ID No: 21 which encodes the amino acid sequence of SEQ ID No: 22. This enzyme is believed to have glutathione peroxidase activity.
  • Sequence Oxid-12 scaffold00031.G1329 is encoded by the nucleotides of SEQ ID No: 23 which encodes the amino acid sequence of SEQ ID No: 24. This enzyme is believed to have D-amino-acid oxidase activity.
  • Sequence Oxid-13 scaflbld00031.G1386 is encoded by the nucleotides of SEQ ID No: 25 which encodes the amino acid sequence of SEQ ID No: 26. This enzyme is believed to have dihydroorotate oxidase activity.
  • Sequence Oxid-14 scaffold00050.G316 is encoded by the nucleotides of SEQ ID No: 27 which encodes the amino acid sequence of SEQ ID No: 28. This enzyme is believed to have linoleate diol synthase activity.
  • Sequence Oxid-15 scaffold00050.G973 is encoded by the nucleotides of SEQ ID No: 29 which encodes the amino acid sequence of SEQ ID No: 30. This enzyme is believed to have cytochrome-c oxidase activity.
  • Sequence Oxid-16 scaffold00050.G992 is encoded by the nucleotides of SEQ ID No: 31 which encodes the amino acid sequence of SEQ ID No: 32. This enzyme is believed to have amine oxidase activity.
  • Sequence Oxid-17 scaffold00050.Gl 115 is encoded by the nucleotides of SEQ ID No: 33 which encodes the amino acid sequence of SEQ ID No: 34. This enzyme is believed to have thiol oxidase activity.
  • Sequence Oxid-18 scaffold00050.Gl 138 is encoded by the nucleotides of SEQ ID No: 35 which encodes the amino acid sequence of SEQ ID No: 36. This enzyme is believed to have thioredoxin peroxidase activity.
  • the enzyme identified as Sequence Oxid-19 scaffold00050.Gl 145 is encoded by the nucleotides of SEQ ID No: 37 which encodes the amino acid sequence of SEQ ID No: 38. This enzyme is believed to have glucose oxidase - alcohol oxidase activity.
  • Sequence Oxid-20 scaffold00050.G1403 is encoded by the nucleotides of SEQ ID No: 39 which encodes the amino acid sequence of SEQ ID No: 40. This enzyme is believed to have Peroxidase activity.
  • Sequence Oxid-21 scaffold00050.G1668 is encoded by the nucleotides of SEQ ID No: 41 which encodes the amino acid sequence of SEQ ID No: 42. This enzyme is believed to have ent-kaurene oxidase activity.
  • Sequence Oxid-22 scaffold00071.G641 is encoded by the nucleotides of SEQ ID No: 43 which encodes the amino acid sequence of SEQ ID No: 44. This enzyme is believed to have oxygen-dependent protoporphyrinogen oxidase activity.
  • Sequence Oxid-23 scaffold00071.G707 is encoded by the nucleotides of SEQ ID No: 45 which encodes the amino acid sequence of SEQ ID No: 46. This enzyme is believed to have cytochrome-c peroxidase activity.
  • Sequence Oxid-24 scaffold00071.G1701 is encoded by the nucleotides of SEQ ID No: 47 which encodes the amino acid sequence of SEQ ID No: 48. This enzyme is believed to have D-amino-acid oxidase activity.
  • Sequence Oxid-25 scaffold00071.G2756 is encoded by the nucleotides of SEQ ID No: 49 which encodes the amino acid sequence of SEQ ID No: 50. This enzyme is believed to have peroxiredoxin activity.
  • Sequence Oxid-26 scaffold00071.G2771 is encoded by the nucleotides of SEQ ID No: 51 which encodes the amino acid sequence of SEQ ID No: 52. This enzyme is believed to have D-arabinono-l,4-lactone oxidase activity.
  • Sequence Oxid-27 scaffold00071.G2849 is encoded by the nucleotides of SEQ ID No: 53 which encodes the amino acid sequence of SEQ ID No: 54. This enzyme is believed to have D-arabinono-l,4-lactone oxidase activity.
  • Sequence Oxid-28 scaffold00071.G3230 is encoded by the nucleotides of SEQ ID No: 55 which encodes the amino acid sequence of SEQ ID No: 56. This enzyme is believed to have cytochrome-c oxidase activity.
  • Sequence Oxid-29 scaffold00071.G3441 is encoded by the nucleotides of SEQ ID No: 56 which encodes the amino acid sequence of SEQ ID No: 58. This enzyme is believed to have alcohol oxidase activity.
  • Sequence Oxid-30 scaffold00071.G3442 is encoded by the nucleotides of SEQ ID No: 57 which encodes the amino acid sequence of SEQ ID No: 60. This enzyme is believed to have alcohol oxidase activity.
  • Sequence Oxid-31 scaffold00071.G3476 is encoded by the nucleotides of SEQ ID No: 59 which encodes the amino acid sequence of SEQ ID No: 62. This enzyme is believed to have amine oxidase activity.
  • Sequence Oxid-32 scaffold00075.G779 is encoded by the nucleotides of SEQ ID No: 61 which encodes the amino acid sequence of SEQ ID No: 64. This enzyme is believed to have C-4 methylsterol oxidase activity.
  • Sequence Oxid-33 scaffold00092.G21 is encoded by the nucleotides of SEQ ID No: 63 which encodes the amino acid sequence of SEQ ID No: 66. This enzyme is believed to have glucose oxidase activity.
  • Sequence Oxid-34 scaffold00122.G43 is encoded by the nucleotides of SEQ ID No: 65 which encodes the amino acid sequence of SEQ ID No: 68. This enzyme is believed to have catalase activity.
  • Sequence Oxid-35 scaffold00122.G57 is encoded by the nucleotides of SEQ ID No: 67 which encodes the amino acid sequence of SEQ ID No: 70. This enzyme is believed to have glucose oxidase - alcohol oxidase activity.
  • Sequence Oxid-36 scaffold00131.G269 is encoded by the nucleotides of SEQ ID No: 69 which encodes the amino acid sequence of SEQ ID No: 72. This enzyme is believed to have dihydroorotate oxidase activity.
  • Sequence Oxid-37 scaffold00131.G1286 is encoded by the nucleotides of SEQ ID No: 71 which encodes the amino acid sequence of SEQ ID No: 74. This enzyme is believed to have cytochrome-c oxidase activity.
  • Sequence Oxid-38 scaffold00131.G1420 is encoded by the nucleotides of SEQ ID No: 73 which encodes the amino acid sequence of SEQ ID No: 76. This enzyme is believed to have phospholipid-hydroperoxide glutathione peroxidase activity.
  • Sequence Oxid-39 scaffold00137.G197 is encoded by the nucleotides of SEQ ID No: 75 which encodes the amino acid sequence of SEQ ID No: 78. This enzyme is believed to have alcohol oxidase activity.
  • Sequence Oxid-40 scaffold00142.G451 is encoded by the nucleotides of SEQ ID No: 77 which encodes the amino acid sequence of SEQ ID No: 80. This enzyme is believed to have cytochrome-c oxidase activity.
  • Sequence Oxid-41 scaffold00169.G174 is encoded by the nucleotides of SEQ ID No: 79 which encodes the amino acid sequence of SEQ ID No: 82. This enzyme is believed to have alcohol oxidase activity.
  • Sequence Oxid-42 scaffold00227.G212 is encoded by the nucleotides of SEQ ID No: 81 which encodes the amino acid sequence of SEQ ID No: 84. This enzyme is believed to have versatile peroxidase activity.
  • Sequence Oxid-43 scaffold00227.G247 is encoded by the nucleotides of SEQ ID No: 83 which encodes the amino acid sequence of SEQ ID No: 86. This enzyme is believed to have L-pipecolate oxidase - proline oxidase activity.
  • Sequence Oxid-44 scaffold00227.G400 is encoded by the nucleotides of SEQ ID No: 85 which encodes the amino acid sequence of SEQ ID No: 88. This enzyme is believed to have ent-kaurene oxidase activity.
  • Sequence Oxid-45 scaffold00050.G1500 is encoded by the nucleotides of SEQ ID No: 87 which encodes the amino acid sequence of SEQ ID No: 87
  • Sequence Oxid-46 177_g is encoded by the nucleotides of
  • SEQ ID No: 89 which encodes the amino acid sequence of SEQ ID No: 92. This enzyme is believed to have ent-kaurene oxidase activity.
  • Sequence Oxid-47 578_g is encoded by the nucleotides of
  • SEQ ID No: 91 which encodes the amino acid sequence of SEQ ID No: 94. This enzyme is believed to have cytochrome-c oxidase activity.
  • Sequence Oxid-48 941_g is encoded by the nucleotides of
  • SEQ ID No: 93 which encodes the amino acid sequence of SEQ ID No: 96. This enzyme is believed to have catalase activity.
  • Sequence Oxid-49 1538_g is encoded by the nucleotides of SEQ ID No: 95 which encodes the amino acid sequence of SEQ ID No: 98.
  • This enzyme is believed to have catalase activity.
  • Sequence Oxid-50 1787_g is encoded by the nucleotides of SEQ ID No: 97 which encodes the amino acid sequence of SEQ ID No: 100.
  • This enzyme is believed to have D-amino-acid oxidase activity.
  • Sequence Oxid-51 2870_g is encoded by the nucleotides of SEQ ID No: 99 which encodes the amino acid sequence of SEQ ID No: 102.
  • This enzyme is believed to have flavin-linked sulfhydryl oxidase activity.
  • Sequence Oxid-52 362 l_g is encoded by the nucleotides of SEQ ID No: 101 which encodes the amino acid sequence of SEQ ID No: 104.
  • This enzyme is believed to have oxygen-dependent protoporphyrinogen oxidase activity.
  • Sequence Oxid-53 5878_g is encoded by the nucleotides of SEQ ID No: 103 which encodes the amino acid sequence of SEQ ID No: 106.
  • This enzyme is believed to have pyridoxamine-phosphate oxidase activity.
  • Sequence Oxid-54 6852_g is encoded by the nucleotides of SEQ ID No: 105 which encodes the amino acid sequence of SEQ ID No: 108.
  • This enzyme is believed to have catalase activity.
  • Sequence Oxid-55 7956_g is encoded by the nucleotides of SEQ ID No: 107 which encodes the amino acid sequence of SEQ ID No: 110.
  • This enzyme is believed to have peroxidase activity.
  • Sequence Oxid-56 8774_g is encoded by the nucleotides of SEQ ID No: 109 which encodes the amino acid sequence of SEQ ID No: 112.
  • This enzyme is believed to have cytochrome-c oxidase activity.
  • Sequence Oxid-58 scaffold00122.pathl .genel6 is encoded by the nucleotides of SEQ ID No: 113 which encodes the amino acid sequence of SEQ ID No: 116. This enzyme is believed to have catalase activity.
  • Sequence Oxid-61 scaffold00050.G998 is encoded by the nucleotides of SEQ ID No: 119 which encodes the amino acid sequence of SEQ ID No: 119
  • Sequence Oxid-62 scaffold00071.G2587 is encoded by the nucleotides of SEQ ID No: 121 which encodes the amino acid sequence of SEQ ID No: 121
  • Sequence Oxid-63 scaffold00169.G454 is encoded by the nucleotides of SEQ ID No: 123 which encodes the amino acid sequence of SEQ ID No: 123
  • Sequence Oxid-64 scaffold00169.G455 is encoded by the nucleotides of SEQ ID No: 125 which encodes the amino acid sequence of SEQ ID No: 125
  • Sequence Oxid-65 scaffold00071.G416 is encoded by the nucleotides of SEQ ID No: 127 which encodes the amino acid sequence of SEQ ID No: 130. This enzyme is believed to have thioredoxin-disulfide reductase activity.
  • Sequence Oxid-66 scaffold00071.G1899 is encoded by the nucleotides of SEQ ID No: 129 which encodes the amino acid sequence of SEQ ID No: 132. This enzyme is believed to have arsenate reductase (glutaredoxin) activity.
  • Sequence Oxid-67 scaffold00075.G497 is encoded by the nucleotides of SEQ ID No: 131 which encodes the amino acid sequence of SEQ ID No: 134. This enzyme is believed to have ferredoxin-NADP+ reductase activity.
  • Sequence Oxid-68 scaffold00031.G158 is encoded by the nucleotides of SEQ ID No: 133 which encodes the amino acid sequence of SEQ ID No: 136. This enzyme is believed to have tryptophan 2,3-dioxygenase activity.
  • Sequence Oxid-69 scaffold00031.G227 is encoded by the nucleotides of SEQ ID No: 135 which encodes the amino acid sequence of SEQ ID No: 138. This enzyme is believed to have sulfonate dioxygenase activity.
  • Sequence Oxid-70 scaffold00031.G625 is encoded by the nucleotides of SEQ ID No: 137 which encodes the amino acid sequence of SEQ ID No: 140. This enzyme is believed to have catechol 1 ,2-dioxygenase activity.
  • Sequence Oxid-71 scaffold00031.G776 is encoded by the nucleotides of SEQ ID No: 139 which encodes the amino acid sequence of SEQ ID No: 142. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-72 scaffold00031.G1607 is encoded by the nucleotides of SEQ ID No: 141 which encodes the amino acid sequence of SEQ ID No: 144. This enzyme is believed to have 2-nitropropane dioxygenase activity.
  • Sequence Oxid-73 scaffold00050.G277 is encoded by the nucleotides of SEQ ID No: 143 which encodes the amino acid sequence of SEQ ID No: 146. This enzyme is believed to have nitric oxide dioxygenase activity.
  • Sequence Oxid-74 scaffold00071.G251 is encoded by the nucleotides of SEQ ID No: 145 which encodes the amino acid sequence of SEQ ID No: 148. This enzyme is believed to have 3-hydroxyanthranilate 3,4-dioxygenase activity.
  • Sequence Oxid-75 scaffold00071.G335 is encoded by the nucleotides of SEQ ID No: 147 which encodes the amino acid sequence of SEQ ID No: 150. This enzyme is believed to have nitric oxide dioxygenase activity.
  • sequence Oxid-76 scaflbld00071.G2259 is encoded by the nucleotides of SEQ ID No: 149 which encodes the amino acid sequence of SEQ ID No: 152. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-77 scaffold00071.G3208 is encoded by the nucleotides of SEQ ID No: 151 which encodes the amino acid sequence of SEQ ID No: 154. This enzyme is believed to have catechol 1 ,2-dioxygenase activity.
  • Sequence Oxid-78 scaffold00075.G417 is encoded by the nucleotides of SEQ ID No: 153 which encodes the amino acid sequence of SEQ ID No: 156. This enzyme is believed to have cysteine dioxygenase activity.
  • Sequence Oxid-79 scaffold00075.G1075 is encoded by the nucleotides of SEQ ID No: 155 which encodes the amino acid sequence of SEQ ID No: 158. This enzyme is believed to have sulfonate dioxygenase activity.
  • Sequence Oxid-80 scaffold00075.G1251 is encoded by the nucleotides of SEQ ID No: 157 which encodes the amino acid sequence of SEQ ID No: 160. This enzyme is believed to have sulfonate dioxygenase activity.
  • Sequence Oxid-81 scaffold00131.G595 is encoded by the nucleotides of SEQ ID No: 159 which encodes the amino acid sequence of SEQ ID No: 162. This enzyme is believed to have trimethyllysine dioxygenase activity.
  • Sequence Oxid-82 scaffold00131.G596 is encoded by the nucleotides of SEQ ID No: 161 which encodes the amino acid sequence of SEQ ID No: 164. This enzyme is believed to have trimethyllysine dioxygenase activity.
  • Sequence Oxid-83 scaffold00131.G810 is encoded by the nucleotides of SEQ ID No: 163 which encodes the amino acid sequence of SEQ ID No: 166. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-84 scaffold00131.G1675 is encoded by the nucleotides of SEQ ID No: 165 which encodes the amino acid sequence of SEQ ID No: 168. This enzyme is believed to have 2-nitropropane dioxygenase activity.
  • Sequence Oxid-85 scaffold00131.G2052 is encoded by the nucleotides of SEQ ID No: 167 which encodes the amino acid sequence of SEQ ID No: 170. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-86 scaffold00131.G2141 is encoded by the nucleotides of SEQ ID No: 169 which encodes the amino acid sequence of SEQ ID No: 172. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-87 scaflbld00142.G307 is encoded by the nucleotides of SEQ ID No: 171 which encodes the amino acid sequence of SEQ ID No: 174. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-88 scaffold00142.G386 is encoded by the nucleotides of SEQ ID No: 173 which encodes the amino acid sequence of SEQ ID No: 176. This enzyme is believed to have tryptophan 2,3-dioxygenase activity.
  • Sequence Oxid-89 scaffold00169.G8 is encoded by the nucleotides of SEQ ID No: 175 which encodes the amino acid sequence of SEQ ID No: 178. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-90 scaffold00169.G77 is encoded by the nucleotides of SEQ ID No: 177 which encodes the amino acid sequence of SEQ ID No: 180. This enzyme is believed to have sulfonate dioxygenase activity.
  • Sequence Oxid-91 scaffold00169.G407 is encoded by the nucleotides of SEQ ID No: 179 which encodes the amino acid sequence of SEQ ID No: 182. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-92 scaffold00227.G198 is encoded by the nucleotides of SEQ ID No: 181 which encodes the amino acid sequence of SEQ ID No: 184. This enzyme is believed to have 2-nitropropane dioxygenase activity.
  • Sequence Oxid-93 scaffold00227.G634 is encoded by the nucleotides of SEQ ID No: 183 which encodes the amino acid sequence of SEQ ID No: 186. This enzyme is believed to have catechol 1 ,2-dioxygenase activity.
  • Sequence Oxid-94 2107_g is encoded by the nucleotides of SEQ ID No: 185 which encodes the amino acid sequence of SEQ ID No: 188. This enzyme is believed to have 2-nitropropane dioxygenase activity.
  • Sequence Oxid-95 977 l_g is encoded by the nucleotides of SEQ ID No: 187 which encodes the amino acid sequence of SEQ ID No: 190. This enzyme is believed to have catechol 1 ,2-dioxygenase activity.
  • Sequence Oxid-96 scaffold00031.G1073 is encoded by the nucleotides of SEQ ID No: 189 which encodes the amino acid sequence of SEQ ID No: 192. This enzyme is believed to have prephenate dehydrogenase (NADP+) activity.
  • Sequence Oxid-97 scaffold00227.G60 is encoded by the nucleotides of SEQ ID No: 191 which encodes the amino acid sequence of SEQ ID No: 194. This enzyme is believed to have homogentisate 1 ,2-dioxygenase activity.
  • Sequence Oxid-98 scaffold00016.G898 is encoded by the nucleotides of SEQ ID No: 193 which encodes the amino acid sequence of SEQ ID No: 196. This enzyme is believed to have 4-hydroxyphenylpyruvate dioxygenase activity.
  • Sequence Oxid-99 scaffold00071.G470 is encoded by the nucleotides of SEQ ID No: 195 which encodes the amino acid sequence of SEQ ID No: 198. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-100 scaffold00071.G607 is encoded by the nucleotides of SEQ ID No: 197 which encodes the amino acid sequence of SEQ ID No: 200. This enzyme is believed to have Protein disulfide oxidoreductase activity.
  • Sequence Oxid-101 scaffold00071.G1052 is encoded by the nucleotides of SEQ ID No: 199 which encodes the amino acid sequence of SEQ ID No: 202. This enzyme is believed to have Protein disulfide isomerase activity.
  • Sequence Oxid-102 scaffold00071.G1584 is encoded by the nucleotides of SEQ ID No: 201 which encodes the amino acid sequence of SEQ ID No: 204. This enzyme is believed to have Protein disulfide isomerase activity.
  • Sequence Oxid-103 scaffold00071.G2169 is encoded by the nucleotides of SEQ ID No: 203 which encodes the amino acid sequence of SEQ ID No: 206. This enzyme is believed to have Protein disulfide oxidoreductase activity.
  • Sequence Oxid-104 scaffold00131.G1597 is encoded by the nucleotides of SEQ ID No: 205 which encodes the amino acid sequence of SEQ ID No: 208. This enzyme is believed to have Protein disulfide isomerase activity.
  • Sequence Oxid-105 scaffold00227.G519 is encoded by the nucleotides of SEQ ID No: 207 which encodes the amino acid sequence of SEQ ID No: 210. This enzyme is believed to have Protein disulfide oxidoreductase activity.
  • Sequence Oxid-106 scaffold00016.G594 is encoded by the nucleotides of SEQ ID No: 209 which encodes the amino acid sequence of SEQ ID No: 212. This enzyme is believed to have Protein disulfide oxidoreductase activity.
  • Sequence Oxid-107 scaffold00016.Gl 163 is encoded by the nucleotides of SEQ ID No: 211 which encodes the amino acid sequence of SEQ ID No: 214. This enzyme is believed to have glutathione-disulfide reductase activity.
  • Sequence Oxid-108 scaflbld00031.G1301 is encoded by the nucleotides of SEQ ID No: 213 which encodes the amino acid sequence of SEQ ID No: 216. This enzyme is believed to have Protein disulfide oxidoreductase activity.
  • Sequence Oxid-109 scaffold00050.G952 is encoded by the nucleotides of SEQ ID No: 215 which encodes the amino acid sequence of SEQ ID No: 218. This enzyme is believed to have Protein disulfide oxidoreductase activity.
  • Sequence Oxid-110 scaffold00031.G37 is encoded by the nucleotides of SEQ ID No: 217 which encodes the amino acid sequence of SEQ ID No: 220. This enzyme is believed to have Redox-active center Protein activity.
  • Sequence Oxid-111 scaffold00031.G200 is encoded by the nucleotides of SEQ ID No: 219 which encodes the amino acid sequence of SEQ ID No: 222. This enzyme is believed to have Redox-active center Protein activity.
  • Sequence Oxid-112 scaffold00031.G1677 is encoded by the nucleotides of SEQ ID No: 221 which encodes the amino acid sequence of SEQ ID No: 224. This enzyme is believed to have Redox-active center Protein activity.
  • Sequence Oxid-113 scaffold00071.G1335 is encoded by the nucleotides of SEQ ID No: 223 which encodes the amino acid sequence of SEQ ID No: 226. This enzyme is believed to have Redox-active center Protein activity.
  • Sequence Oxid-114 scaffold00071.G3221 is encoded by the nucleotides of SEQ ID No: 225 which encodes the amino acid sequence of SEQ ID No: 228. This enzyme is believed to have Redox-active center Protein activity.
  • Sequence Oxid-115 scaffold00129.Gl is encoded by the nucleotides of SEQ ID No: 227 which encodes the amino acid sequence of SEQ ID No: 230. This enzyme is believed to have Redox-active center Protein activity.
  • Sequence Oxid-116 scaffold00142.G269 is encoded by the nucleotides of SEQ ID No: 229 which encodes the amino acid sequence of SEQ ID No: 232. This enzyme is believed to have Redox-active center Protein activity.
  • Sequence Oxid-117 scaffold00071.G3389 is encoded by the nucleotides of SEQ ID No: 231 which encodes the amino acid sequence of SEQ ID No: 234. This enzyme is believed to have cellobiose dehydrogenase (acceptor) activity.
  • Sequence Oxid-118 scaflbld00092.G499 is encoded by the nucleotides of SEQ ID No: 233 which encodes the amino acid sequence of SEQ ID No: 236. This enzyme is believed to have cellobiose dehydrogenase (acceptor) activity.
  • Sequence Oxid-119 scaffold00137.G80 is encoded by the nucleotides of SEQ ID No: 235.
  • SEQ ID No: 237 which encodes the amino acid sequence of SEQ ID No: 238. This enzyme is believed to have cellobiose dehydrogenase (acceptor) activity.
  • Sequence Oxid-120 scaffold00142.G279 is encoded by the nucleotides of SEQ ID No: 239 which encodes the amino acid sequence of SEQ ID No: 240. This enzyme is believed to have cellobiose dehydrogenase (acceptor) activity.
  • Sequence Oxid-121 scaffold00142.G282 is encoded by the nucleotides of SEQ ID No: 241 which encodes the amino acid sequence of SEQ ID No: 242. This enzyme is believed to have cellobiose dehydrogenase (acceptor) activity.
  • Sequence Oxid-122 scaffold00050.G528 is encoded by the nucleotides of SEQ ID No: 243 which encodes the amino acid sequence of SEQ ID No: 244. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-123 scaffold00050.Gl 147 is encoded by the nucleotides of SEQ ID No: 245 which encodes the amino acid sequence of SEQ ID No: 246. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-124 scaffold00071.G1252 is encoded by the nucleotides of SEQ ID No: 247 which encodes the amino acid sequence of SEQ ID No: 248. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-125 scaffold00092.G8 is encoded by the nucleotides of SEQ ID No: 249 which encodes the amino acid sequence of SEQ ID No: 250. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-126 scaffold00092.G500 is encoded by the nucleotides of SEQ ID No: 251 which encodes the amino acid sequence of SEQ ID No: 252. This enzyme is believed to have cellobiose dehydrogenase (acceptor) activity.
  • Sequence Oxid-127 scaflbld00122.G120 is encoded by the nucleotides of SEQ ID No: 253 which encodes the amino acid sequence of SEQ ID No: 254. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-128 scaffold00131.G1200 is encoded by the nucleotides of SEQ ID No: 255 which encodes the amino acid sequence of SEQ ID No: 256. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-129 scaffold00169.G243 is encoded by the nucleotides of SEQ ID No: 257 which encodes the amino acid sequence of SEQ ID No: 258. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-130 scaffold00169.G244 is encoded by the nucleotides of SEQ ID No: 259 which encodes the amino acid sequence of SEQ ID No: 260. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-131 scaffold00131.G1201 is encoded by the nucleotides of SEQ ID No: 261 which encodes the amino acid sequence of SEQ ID No: 262. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-132 scaffold00050.G593 is encoded by the nucleotides of SEQ ID No: 263 which encodes the amino acid sequence of SEQ ID No: 264. This enzyme is believed to have nitrate reductase (NADPH) activity.
  • NADPH nitrate reductase
  • Sequence Oxid-133 scaffold00075.G929 is encoded by the nucleotides of SEQ ID No: 265 which encodes the amino acid sequence of SEQ ID No: 266. This enzyme is believed to have nitrate reductase (NADPH) activity.
  • NADPH nitrate reductase
  • Sequence Oxid-134 scaffold00227.G302 is encoded by the nucleotides of SEQ ID No: 267 which encodes the amino acid sequence of SEQ ID No: 268. This enzyme is believed to have nitrite reductase [NAD(P)H] activity.
  • Sequence Oxid-135 scaffold00131.G1207 is encoded by the nucleotides of SEQ ID No: 269 which encodes the amino acid sequence of SEQ ID No: 270. This enzyme is believed to have nitrite reductase (NO-forming) activity.
  • Sequence Oxid-136 scaffold00016.G461 is encoded by the nucleotides of SEQ ID No: 271 which encodes the amino acid sequence of SEQ ID No: 272. This enzyme is believed to have glutamate synthase (NADPH) activity.
  • Sequence Oxid-137 scaffold00031.G1084 is encoded by the nucleotides of SEQ ID No: 273 which encodes the amino acid sequence of SEQ ID No: 274. This enzyme is believed to have glutamate dehydrogenase activity.
  • Sequence Oxid-138 scaffold00050.G21 is encoded by the nucleotides of SEQ ID No: 275 which encodes the amino acid sequence of SEQ ID No: 276. This enzyme is believed to have proline dehydrogenase activity.
  • Sequence Oxid-139 scaffold00075.G589 is encoded by the nucleotides of SEQ ID No: 277 which encodes the amino acid sequence of SEQ ID No: 278. This enzyme is believed to have isocitrate dehydrogenase (NAD+) activity.
  • CCL09102 is encoded by the nucleotides of SEQ ID No: 279 which encodes the amino acid sequence of SEQ ID No: 280. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-142 scaffold00016.G1047 (CL09554) is encoded by the nucleotides of SEQ ID No: 283 which encodes the amino acid sequence of SEQ ID No: 284. This enzyme is believed to have monooxygenase activity.
  • scaffold00019.pathl .gene7 (CL05569) is encoded by the nucleotides of SEQ ID No: 285 which encodes the amino acid sequence of SEQ ID No: 286. This enzyme is believed to have oxidation reduction activity.
  • scaffold00022.pathl .genel (CL08704) is encoded by the nucleotides of SEQ ID No: 287 which encodes the amino acid sequence of SEQ ID No: 288. This enzyme is believed to have oxidation reduction activity.
  • scaffold00031.pathl .genel4 (CL07195) is encoded by the nucleotides of SEQ ID No: 289 which encodes the amino acid sequence of SEQ ID No: 290. This enzyme is believed to have oxidation reduction activity. [0268] The enzyme identified as Sequence Oxid-146 scaffold00031.G86 976_g
  • scafFold00031.pathl .gene42 (CL07149) is encoded by the nucleotides of SEQ ID No: 291 which encodes the amino acid sequence of SEQ ID No: 292. This enzyme is believed to have oxidation reduction activity.
  • scaffold00031.pathl .gene69 (CL07117) is encoded by the nucleotides of SEQ ID No: 293 which encodes the amino acid sequence of SEQ ID No: 294. This enzyme is believed to have oxidation reduction activity.
  • the enzyme identified as Sequence Oxid-148 scaffold00031.G225 1063 9 (partly), scaffold00031. pathl . genel 15 (CL07057) is encoded by the nucleotides of SEQ ID No: 295 which encodes the amino acid sequence of SEQ ID No: 296. This enzyme is believed to have oxidation reduction activity.
  • pathl . gene252 (CL06915) is encoded by the nucleotides of SEQ ID No: 297 which encodes the amino acid sequence of SEQ ID No: 298. This enzyme is believed to have regulation of transcription, DNA-dependent activity.
  • pathl . gene263 (CL06904) is encoded by the nucleotides of SEQ ID No: 299 which encodes the amino acid sequence of SEQ ID No: 300. This enzyme is believed to have oxidation reduction activity.
  • pathl . gene521 (CL06630) is encoded by the nucleotides of SEQ ID No: 301 which encodes the amino acid sequence of SEQ ID No: 302. This enzyme is believed to have oxidation reduction activity.
  • pathl . gene780 (CL02845) is encoded by the nucleotides of SEQ ID No: 303 which encodes the amino acid sequence of SEQ ID No: 304. This enzyme is believed to have oxidation reduction activity.
  • pathl . gene783 (CL02839) is encoded by the nucleotides of SEQ ID No: 305 which encodes the amino acid sequence of SEQ ID No: 306. This enzyme is believed to have oxidation reduction activity.
  • the enzyme identified as Sequence Oxid-154 scaffold00050.G279 1167_g, 2268_g, 2269_g, scaffold00050.pathl .genel l6, scaffold00050.pathl .genel l7 (CL10139 CL10141 CL10140) is encoded by the nucleotides of SEQ ID No: 307 which encodes the amino acid sequence of SEQ ID No: 308. This enzyme is believed to have oxidation reduction activity.
  • the enzyme identified as Sequence Oxid-155 scaffold00050.G298 2280_g, 228 l_g, scaffold00050.pathl .genel29, scaffold00050.pathl .genel30 (CL10151) is encoded by the nucleotides of SEQ ID No: 309 which encodes the amino acid sequence of SEQ ID No: 310. This enzyme is believed to have oxidation reduction activity.
  • Sequence Oxid-156 scaffold00050.G308 (CL10159) is encoded by the nucleotides of SEQ ID No: 311 which encodes the amino acid sequence of SEQ ID No: 312. This enzyme is believed to have monooxygenase activity.
  • the enzyme identified as Sequence Oxid-157 scaffold00050.G397 234 l_g, 2342_g, scaffold00050.pathl .genel87 (CL10213) is encoded by the nucleotides of SEQ ID No: 313 which encodes the amino acid sequence of SEQ ID No: 314. This enzyme is believed to have oxidation reduction activity.
  • scaffold00050.pathl .gene246 (CL10291) is encoded by the nucleotides of SEQ ID No: 315 which encodes the amino acid sequence of SEQ ID No: 316. This enzyme is believed to have flavin-containing monooxygenase activity.
  • scaffold00050.pathl .gene325 (CL10405) is encoded by the nucleotides of SEQ ID No: 317 which encodes the amino acid sequence of SEQ ID No: 318. This enzyme is believed to have kynurenine 3 -monooxygenase activity.
  • scaffold00050.pathl .gene425 (CL10527) is encoded by the nucleotides of SEQ ID No: 319 which encodes the amino acid sequence of SEQ ID No: 320. This enzyme is believed to have flavin-containing monooxygenase activity.
  • scaffold00050.pathl .gene511 (CL10614) is encoded by the nucleotides of SEQ ID No: 321 which encodes the amino acid sequence of SEQ ID No: 322. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-162 scaffold00050.G1041 2727_g scaffold00050.pathl .gene514 is encoded by the nucleotides of SEQ ID No: 323 which encodes the amino acid sequence of SEQ ID No: 324. This enzyme is believed to have monooxygenase activity.
  • scaffold00050.pathl .gene515 (CL10617) is encoded by the nucleotides of SEQ ID NO: 1
  • scaffold00050.pathl .gene702 (CL10825) is encoded by the nucleotides of SEQ ID NO: 1
  • scaffold00050.pathl .gene747 (CL10883) is encoded by the nucleotides of SEQ ID NO: 1
  • scaffold00050.pathl .gene821 (CL10975) is encoded by the nucleotides of SEQ ID NO: 1
  • scaffold00050.pathl .gene838 (CL10989) is encoded by the nucleotides of SEQ ID NO: 1
  • scaffold00053.pathl .genel3 (CL11584) is encoded by the nucleotides of SEQ ID NO: 1
  • scaffold00053.pathl .gene26 (CL11558) is encoded by the nucleotides of SEQ ID NO: 1
  • scaffold00071.pathl .gene427 (CL00475 CL00476) is encoded by the nucleotides of SEQ ID No: 339 which encodes the amino acid sequence of SEQ ID No: 340. This enzyme is believed to have phenol 2-monooxygenase activity.
  • scaffold00071.pathl .gene427 is encoded by the nucleotides of SEQ ID No: 341 which encodes the amino acid sequence of SEQ ID No: 342. This enzyme is believed to have monooxygenase activity.
  • scaffold00071.pathl .gene459 (CL00510) is encoded by the nucleotides of SEQ ID No: 343 which encodes the amino acid sequence of SEQ ID No: 344. This enzyme is believed to have monophenol monooxygenase activity.
  • scaffold00071.pathl .gene492 (CL00537) is encoded by the nucleotides of SEQ ID No: 345 which encodes the amino acid sequence of SEQ ID No: 346. This enzyme is believed to have dopamine beta-monooxygenase activity.
  • scaffold00071.pathl .gene580 (CL00621) is encoded by the nucleotides of SEQ ID No: 347 which encodes the amino acid sequence of SEQ ID No: 348. This enzyme is believed to have monooxygenase activity.
  • scaffold00071.pathl .gene621 (CL00688 CL08353 CL01496 CL03246) is encoded by the nucleotides of SEQ ID No: 349 which encodes the amino acid sequence of SEQ ID No: 350. This enzyme is believed to have flavin-containing
  • scaffold00071.pathl .gene875 (CL08558 CL00981) is encoded by the nucleotides of SEQ ID No: 351 which encodes the amino acid sequence of SEQ ID No: 352. This enzyme is believed to have flavin-containing monooxygenase activity.
  • scaffold00071.pathl .genel020 (CL01129) is encoded by the nucleotides of SEQ ID No: 353 which encodes the amino acid sequence of SEQ ID No: 354. This enzyme is believed to have lactate 2-monooxygenase activity.
  • scaffold00071.pathl .genel 187, scaffold00071.pathl .genel 188 (CL07578 CL01313) is encoded by the nucleotides of SEQ ID No: 355 which encodes the amino acid sequence of SEQ ID No: 356. This enzyme is believed to have monooxygenase activity.
  • scaffold00071.pathl .genel245, scaffold00071.pathl .genel246 (CL01384) is encoded by the nucleotides of SEQ ID No: 357 which encodes the amino acid sequence of SEQ ID No: 358. This enzyme is believed to have flavin-containing monooxygenase activity.
  • scaffold00071.pathl .genel812 (CL11406) is encoded by the nucleotides of SEQ ID No: 359 which encodes the amino acid sequence of SEQ ID No: 360. This enzyme is believed to have monooxygenase activity.
  • scaffold00075.pathl .gene356 (CL03408) is encoded by the nucleotides of SEQ ID No: 361 which encodes the amino acid sequence of SEQ ID No: 362. This enzyme is believed to have monooxygenase activity.
  • scaffold00075.pathl .gene444 (CL03483) is encoded by the nucleotides of SEQ ID No: 363 which encodes the amino acid sequence of SEQ ID No: 364. This enzyme is believed to have squalene monooxygenase activity.
  • scaffold00075.pathl .gene459 (CL03497) is encoded by the nucleotides of SEQ ID No: 365 which encodes the amino acid sequence of SEQ ID No: 366. This enzyme is believed to have deoxyhypusine monooxygenase activity.
  • Sequence Oxid-184 scaffold00092.G3 (CL05970) is encoded by the nucleotides of SEQ ID No: 367 which encodes the amino acid sequence of SEQ ID No: 368. This enzyme is believed to have monooxygenase activity.
  • scaffold00092.pathl .gene20 (CL05985) is encoded by the nucleotides of SEQ ID No: 369 which encodes the amino acid sequence of SEQ ID No: 370. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-186 scaffold00092.G71 6289_g scaffold00092.pathl .gene41 (CL06010) is encoded by the nucleotides of SEQ ID No: 371 which encodes the amino acid sequence of SEQ ID No: 372. This enzyme is believed to have monooxygenase activity.
  • scaffold00092.pathl .gene42 (CL06011 CL06012) is encoded by the nucleotides of SEQ ID No: 373 which encodes the amino acid sequence of SEQ ID No: 374. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-188 scaffold00092.G89 (CL06022) is encoded by the nucleotides of SEQ ID No: 375 which encodes the amino acid sequence of SEQ ID No: 376. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-189 scaffold00092.G158 (CL06068) is encoded by the nucleotides of SEQ ID No: 377 which encodes the amino acid sequence of SEQ ID No: 378. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-190 scaffold00092.G332 (CL06172) is encoded by the nucleotides of SEQ ID No: 379 which encodes the amino acid sequence of SEQ ID No: 380. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-191 scaffold00105.G2 is encoded by the nucleotides of SEQ ID No: 381 which encodes the amino acid sequence of SEQ ID No: 382. This enzyme is believed to have flavin-containing monooxygenase activity.
  • Sequence Oxid-193 scaffold00120.Gl is encoded by the nucleotides of SEQ ID No: 385 which encodes the amino acid sequence of SEQ ID No: 386. This enzyme is believed to have monooxygenase activity.
  • CL03832 is encoded by the nucleotides of SEQ ID No: 389 which encodes the amino acid sequence of SEQ ID No: 390. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-196 scaffold00122.G80 (CL03841) is encoded by the nucleotides of SEQ ID No: 391 which encodes the amino acid sequence of SEQ ID No: 392. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-197 scaffold00122.G198 (CL03918) is encoded by the nucleotides of SEQ ID No: 393 which encodes the amino acid sequence of SEQ ID No: 394. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-198 scaffold00122.G199 (CL03919) is encoded by the nucleotides of SEQ ID No: 395 which encodes the amino acid sequence of SEQ ID No: 396. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-199 scaffold00122.G202 (CL03922) is encoded by the nucleotides of SEQ ID No: 397 which encodes the amino acid sequence of SEQ ID No: 398. This enzyme is believed to have monooxygenase activity.
  • CL04267 is encoded by the nucleotides of SEQ ID No: 399 which encodes the amino acid sequence of 400. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-201 scaffold00131.G363 is encoded by the nucleotides of SEQ ID No: 401 which encodes the amino acid sequence of SEQ ID No: 402. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-202 scaffold00131.G364 (CL04453) is encoded by the nucleotides of SEQ ID No: 403 which encodes the amino acid sequence of SEQ ID No: 404. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-203 scaffold00131.G446 is encoded by the nucleotides of SEQ ID No: 405 which encodes the amino acid sequence of SEQ ID No: 406. This enzyme is believed to have flavin-containing monooxygenase activity CL04492 activity.
  • Sequence Oxid-204 scaffold00131.G752 (CL04674) is encoded by the nucleotides of SEQ ID No: 407 which encodes the amino acid sequence of SEQ ID No: 408. This enzyme is believed to have phenol 2- monooxygenase activity.
  • Sequence Oxid-205 scaffold00131.G875 (CL04745) is encoded by the nucleotides of SEQ ID No: 409 which encodes the amino acid sequence of SEQ ID No: 410. This enzyme is believed to have flavin-containing monooxygenase activity.
  • CL04796 is encoded by the nucleotides of SEQ ID No: 411 which encodes the amino acid sequence of SEQ ID No: 412. This enzyme is believed to have benzoate 4-monooxygenase activity.
  • Sequence Oxid-207 scaffold00131.Gl 166 (CL04936) is encoded by the nucleotides of SEQ ID No: 413 which encodes the amino acid sequence of SEQ ID No: 414. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-208 scaffold00131.G1265 is encoded by the nucleotides of SEQ ID No: 415 which encodes the amino acid sequence of SEQ ID No: 416. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-209 scaffold00131.G1806 (CL05310) is encoded by the nucleotides of SEQ ID No: 417 which encodes the amino acid sequence of SEQ ID No: 418. This enzyme is believed to have monooxygenase activity.
  • CL05012 CL08560 CL08557) is encoded by the nucleotides of SEQ ID No: 419 which encodes the amino acid sequence of SEQ ID No: 420. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-211 scaffold00142.G368 is encoded by the nucleotides of SEQ ID No: 421 which encodes the amino acid sequence of SEQ ID No: 422. This enzyme is believed to have flavin-containing monooxygenase activity.
  • Sequence Oxid-212 scaflbld00142.G370 is encoded by the nucleotides of SEQ ID No: 423 which encodes the amino acid sequence of SEQ ID No: 424. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-213 scaffold00142.G465 (CL08624) is encoded by the nucleotides of SEQ ID No: 425 which encodes the amino acid sequence of SEQ ID No: 426. This enzyme is believed to have monooxygenase activity.
  • CL05970 is encoded by the nucleotides of SEQ ID No: 427 which encodes the amino acid sequence of SEQ ID No: 428. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-215 scaffold00142.G589 is encoded by the nucleotides of SEQ ID No: 429 which encodes the amino acid sequence of SEQ ID No: 430. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-216 scaffold00169.G30 (CL07907) is encoded by the nucleotides of SEQ ID No: 431 which encodes the amino acid sequence of SEQ ID No: 432. This enzyme is believed to have monooxygenase activity.
  • CL11558 CL07902 is encoded by the nucleotides of SEQ ID No: 433 which encodes the amino acid sequence of SEQ ID No: 434. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-218 scaffold00169.G288 (CL07724) is encoded by the nucleotides of SEQ ID No: 435 which encodes the amino acid sequence of SEQ ID No: 436. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-219 scaffold00169.G316 (CL07703) is encoded by the nucleotides of SEQ ID No: 437 which encodes the amino acid sequence of SEQ ID No: 438. This enzyme is believed to have flavin-containing monooxygenase activity.
  • Sequence Oxid-220 scaffold00169.G318 (CL07702) is encoded by the nucleotides of SEQ ID No: 439 which encodes the amino acid sequence of SEQ ID No: 440. This enzyme is believed to have flavin-containing monooxygenase activity.
  • Sequence Oxid-221 scaffold00169.G321 (CL07700) is encoded by the nucleotides of SEQ ID No: 441 which encodes the amino acid sequence of SEQ ID No: 442. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-222 scaffold00169.G325 (CL07697) is encoded by the nucleotides of SEQ ID No: 443 which encodes the amino acid sequence of SEQ ID No: 444. This enzyme is believed to have flavin-containing monooxygenase activity.
  • Sequence Oxid-223 scaffold00169.G402 (CL07634) is encoded by the nucleotides of SEQ ID No: 445 which encodes the amino acid sequence of SEQ ID No: 446. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-224 scaffold00169.G442 (CL07610) is encoded by the nucleotides of SEQ ID No: 447 which encodes the amino acid sequence of SEQ ID No: 448. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-225 scaffold00214.G9 (CL02472) is encoded by the nucleotides of SEQ ID No: 449 which encodes the amino acid sequence of SEQ ID No: 450. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-226 scaffold00214.G10 is encoded by the nucleotides of SEQ ID No: 451 which encodes the amino acid sequence of SEQ ID No: 452. This enzyme is believed to have monooxygenase activity.
  • CL02481) is encoded by the nucleotides of SEQ ID No: 453 which encodes the amino acid sequence of SEQ ID No: 454. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-228 scaffold00227.G204 (CL08208) is encoded by the nucleotides of SEQ ID No: 455 which encodes the amino acid sequence of SEQ ID No: 456. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-229 scaffold00227.G683 (CL08810
  • CL07938 is encoded by the nucleotides of SEQ ID No: 457 which encodes the amino acid sequence of SEQ ID No: 458. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-230 scaffold00050.G1657 (CL11016) is encoded by the nucleotides of SEQ ID No: 459 which encodes the amino acid sequence of SEQ ID No: 460. This enzyme is believed to have NADPH-hemo Protein reductase activity.
  • Sequence Oxid-231 scaffold00068.Gl is encoded by the nucleotides of SEQ ID No: 461 which encodes the amino acid sequence of SEQ ID No: 462. This enzyme is believed to have oxidoreductase activity, acting on paired activity.
  • Sequence Oxid-232 scaffold00071.G571 (CL00326) is encoded by the nucleotides of SEQ ID No: 463 which encodes the amino acid sequence of SEQ ID No: 464. This enzyme is believed to have sterol 14- demethylase activity.
  • Sequence Oxid-233 scaffold00071.G633 is encoded by the nucleotides of SEQ ID No: 465 which encodes the amino acid sequence of SEQ ID No: 466. This enzyme is believed to have oxidoreductase activity, acting on paired d activity.
  • Sequence Oxid-234 scaffold00071.G634 (CL00365) is encoded by the nucleotides of SEQ ID No: 467 which encodes the amino acid sequence of SEQ ID No: 468. This enzyme is believed to have oxidoreductase activity, acting on paired d activity.
  • Sequence Oxid-235 scaffold00071.G1361 (CL00816) is encoded by the nucleotides of SEQ ID No: 469 which encodes the amino acid sequence of SEQ ID No: 470. This enzyme is believed to have oxidoreductase activity, acting on paired d activity.
  • Sequence Oxid-236 scaffold00071.G1662 (CL00992) is encoded by the nucleotides of SEQ ID No: 471 which encodes the amino acid sequence of SEQ ID No: 472. This enzyme is believed to have oxidoreductase activity, acting on paired d activity.
  • Sequence Oxid-237 scaffold00071.G1865 (CLOU 14) is encoded by the nucleotides of SEQ ID No: 473 which encodes the amino acid sequence of SEQ ID No: 474. This enzyme is believed to have oxidoreductase activity, acting on paired d activity.
  • Sequence Oxid-238 scaffold00092.G20 (CL05975) is encoded by the nucleotides of SEQ ID No: 475 which encodes the amino acid sequence of SEQ ID No: 476. This enzyme is believed to have NADPH-hemo Protein reductase activity.
  • CL06087) is encoded by the nucleotides of SEQ ID No: 477 which encodes the amino acid sequence of SEQ ID No: 478. This enzyme is believed to have oxidoreductase activity, acting on paired d activity.
  • Sequence Oxid-240 scaffold00227.G102 (CL08261) is encoded by the nucleotides of SEQ ID No: 479 which encodes the amino acid sequence of SEQ ID No: 480. This enzyme is believed to have oxidoreductase activity, acting on paired d activity.
  • Sequence Oxid-241 scaffold00071.G592 (CL00336) is encoded by the nucleotides of SEQ ID No: 481 which encodes the amino acid sequence of SEQ ID No: 482. This enzyme is believed to have succinate dehydrogenase (ubiquinone) activity
  • Sequence Oxid-242 scaffold00142.G540 (CL08666) is encoded by the nucleotides of SEQ ID No: 483 which encodes the amino acid sequence of SEQ ID No: 484. This enzyme is believed to have activity.
  • Sequence Oxid-243 scaffold00031.G1485 (CL02772) is encoded by the nucleotides of SEQ ID No: 485 which encodes the amino acid sequence of SEQ ID No: 486. This enzyme is believed to have chitin binding activity.
  • Sequence Oxid-244 scaffold00071.G1644 (CL00980) is encoded by the nucleotides of SEQ ID No: 487 which encodes the amino acid sequence of SEQ ID No: 488. This enzyme is believed to have ATP-binding Hydrolase Lipid degradation activity.
  • Sequence Oxid-245 scaffold00131.G793 (CL04696) is encoded by the nucleotides of SEQ ID No: 489 which encodes the amino acid sequence of SEQ ID No: 490. This enzyme is believed to have oxidoreductase activity, Glucooligosaccharide oxidase putative activity.
  • Sequence Oxid-246 scaffold00131.G1570 (CL05186) is encoded by the nucleotides of SEQ ID No: 491 which encodes the amino acid sequence of SEQ ID No: 492. This enzyme is believed to have defense response to fungus and seems to be a combination between manganese peroxidase (MnP) and a (number of) carbohydrate binding domain(s). activity.
  • MnP manganese peroxidase
  • Sequence Oxid-247 scaffold00169.G412 (CL07628) is encoded by the nucleotides of SEQ ID No: 493 which encodes the amino acid sequence of SEQ ID No: 494. This enzyme is believed to have oxidoreductase activity, lactose oxidase activity.
  • C04696 is encoded by the nucleotides of SEQ ID No: 495 which encodes the amino acid sequence of SEQ ID No: 496. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-249 scaffold00092.G538 (CL06304) is encoded by the nucleotides of SEQ ID No: 497 which encodes the amino acid sequence of SEQ ID No: 498. This enzyme is believed to have cytochrome-b5 reductase activity.
  • CL 10276 is encoded by the nucleotides of SEQ ID No: 499 which encodes the amino acid sequence of 500. This enzyme is believed to have oxidoreductase activity.
  • C10276 is encoded by the nucleotides of SEQ ID No: 501 which encodes the amino acid sequence of SEQ ID No: 502. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-252 scaffold00071.G2538 (CL01543) is encoded by the nucleotides of SEQ ID No: 503 which encodes the amino acid sequence of SEQ ID No: 504. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-253 scaffold00131.G2158 (CL05501) is encoded by the nucleotides of SEQ ID No: 505 which encodes the amino acid sequence of SEQ ID No: 506. This enzyme is believed to have copper ion binding, oxidoreductase activity, Laccase activity.
  • Sequence Oxid-254 scaffold00142.G37 (CL08347) is encoded by the nucleotides of SEQ ID No: 507 which encodes the amino acid sequence of SEQ ID No: 508. This enzyme is believed to have copper ion binding, oxidoreductase activity.
  • Sequence Oxid-255 scaffold00016.G641 (CL09304) is encoded by the nucleotides of SEQ ID No: 509 which encodes the amino acid sequence of SEQ ID No: 510. This enzyme is believed to have glycerol-3- phosphate dehydrogenase activity.
  • CL06843 (Cytochrome) is encoded by the nucleotides of SEQ ID No: 511 which encodes the amino acid sequence of SEQ ID No: 512. This enzyme is believed to have D-lactate dehydrogenase activity.
  • CL06797 is encoded by the nucleotides of SEQ ID No: 513 which encodes the amino acid sequence of SEQ ID No: 514. This enzyme is believed to have FAD
  • Flavo Protein, tRNA dihydrouridine synthase activity Flavo Protein, tRNA dihydrouridine synthase activity.
  • Sequence Oxid-259 scaffold00031.G1052 (CL06572) activity is encoded by the nucleotides of SEQ ID No: 517 which encodes the amino acid sequence of SEQ ID No: 518. This enzyme is believed to have FAD Flavo
  • Sequence Oxid-260 scaffold00031.Gl 199 (CL02964) is encoded by the nucleotides of SEQ ID No: 519 which encodes the amino acid sequence of SEQ ID No: 520. This enzyme is believed to have FMN Flavo Protein,
  • Sequence Oxid-261 scaffold00031.G1320 (CL02890) is encoded by the nucleotides of SEQ ID No: 521 which encodes the amino acid sequence of SEQ ID No: 522. This enzyme is believed to have FAD flavo protein, acyl-CoA dehydrogenase activity.
  • Sequence Oxid-262 scaffold00050.G972 (CL10574) is encoded by the nucleotides of SEQ ID No: 523 which encodes the amino acid sequence of SEQ ID No: 524. This enzyme is believed to have FAD flavo protein, acyl-CoA dehydrogenase activity.
  • Sequence Oxid-263 scaffold00050.G1269 (CL10770) is encoded by the nucleotides of SEQ ID No: 525 which encodes the amino acid sequence of SEQ ID No: 526. This enzyme is believed to have disulfide bond FAD Flavo Protein, dihydrolipoyl dehydrogenase activity.
  • Sequence Oxid-264 scaffold00050.G1295 (CL10786) is encoded by the nucleotides of SEQ ID No: 527 which encodes the amino acid sequence of SEQ ID No: 528. This enzyme is believed to have electron transport FAD flavo protein mitochondrion respiratory chain transit peptide transport activity.
  • Sequence Oxid-265 scaffold00050.G1321 (CL10801) is encoded by the nucleotides of SEQ ID No: 529 which encodes the amino acid sequence of SEQ ID No: 530. This enzyme is believed to have FMN Flavo Protein, photoreceptor activity, transcription factor activity.
  • Sequence Oxid-266 scaffold00050.G1429 (CL10879) is encoded by the nucleotides of SEQ ID No: 531 which encodes the amino acid sequence of SEQ ID No: 532. This enzyme is believed to have Cadmium Disulfide bond FAD Flavo Protein, oxidoreductase activity, acting on sulfur g activity.
  • Sequence Oxid-267 scaffold00071.G59 (CL00036) is encoded by the nucleotides of SEQ ID No: 533 which encodes the amino acid sequence of SEQ ID No: 534. This enzyme is believed to have FAD Flavo Protein, D-lactate dehydrogenase (Cytochrome) activity.
  • Sequence Oxid-268 scaffold00071.G556 (CL00318) is encoded by the nucleotides of SEQ ID No: 535 which encodes the amino acid sequence of SEQ ID No: 536. This enzyme is believed to have Cytoplasm FAD Flavo Protein, tRNA dihydrouridine synthase activity.
  • Sequence Oxid-269 scaffold00071.G744 (CL00443) is encoded by the nucleotides of SEQ ID No: 537 which encodes the amino acid sequence of SEQ ID No: 538. This enzyme is believed to have Cytoplasm FAD Flavo Protein, tRNA dihydrouridine synthase activity.
  • Sequence Oxid-270 scaffold00071.G1440 (CL00865) is encoded by the nucleotides of SEQ ID No: 539 which encodes the amino acid sequence of SEQ ID No: 540. This enzyme is believed to have FMN Flavo Protein, NADPH dehydrogenase activity.
  • Sequence Oxid-271 scaffold00071.G2386 (CL01424) is encoded by the nucleotides of SEQ ID No: 541 which encodes the amino acid sequence of SEQ ID No: 542. This enzyme is believed to have heme binding, L- lactate dehydrogenase (Cytochrome) activity.
  • Sequence Oxid-272 scaffold00071.G2604 (CL01584) is encoded by the nucleotides of SEQ ID No: 543 which encodes the amino acid sequence of SEQ ID No: 544. This enzyme is believed to have FMN binding activity.
  • Sequence Oxid-273 scaffold00075.G430 (CL03269) is encoded by the nucleotides of SEQ ID No: 545 which encodes the amino acid sequence of SEQ ID No: 546. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-274 scaffold00075.G449 (CL03284) is encoded by the nucleotides of SEQ ID No: 547 which encodes the amino acid sequence of SEQ ID No: 548.
  • This enzyme is believed to have FAD FMN Flavo Protein, oxidoreductase activity TAH18 GIBZE
  • Sequence Oxid-275 scaffold00075.G652 (CL03401) is encoded by the nucleotides of SEQ ID No: 549 which encodes the amino acid sequence of SEQ ID No: 550. This enzyme is believed to have NADPH-hemo Protein reductase activity.
  • Sequence Oxid-276 scaffold00075.G818 (Cytochrome) is encoded by the nucleotides of SEQ ID No: 551 which encodes the amino acid sequence of SEQ ID No: 552. This enzyme is believed to have L-lactate dehydrogenase (Cytochrome) activity.
  • Sequence Oxid-277 scaffold00092.G461 (CL06252) is encoded by the nucleotides of SEQ ID No: 553 which encodes the amino acid sequence of SEQ ID No: 554. This enzyme is believed to have FMN binding activity.
  • Sequence Oxid-278 scaffold00131.G685 (CL04637) is encoded by the nucleotides of SEQ ID No: 555 which encodes the amino acid sequence of SEQ ID No: 556. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-279 scaffold00131.G729 (CL04661) is encoded by the nucleotides of SEQ ID No: 557 which encodes the amino acid sequence of SEQ ID No: 558. This enzyme is believed to have FMN binding activity.
  • Sequence Oxid-280 scaffold00131.G1632 (CL05218) is encoded by the nucleotides of SEQ ID No: 559 which encodes the amino acid sequence of SEQ ID No: 560. This enzyme is believed to have heme binding, L- lactate dehydrogenase (Cytochrome) activity.
  • Sequence Oxid-281 scaffold00214.G35 (CL02491) is encoded by the nucleotides of SEQ ID No: 561 which encodes the amino acid sequence of SEQ ID No: 562. This enzyme is believed to have FMN binding activity.
  • Sequence Oxid-282 scaffold00214.G73 (CL02521) is encoded by the nucleotides of SEQ ID No: 563 which encodes the amino acid sequence of SEQ ID No: 564. This enzyme is believed to have heme binding, sulfite reductase (NADPH)
  • Sequence Oxid-283 scaffold00227.G366 (CL08117) is encoded by the nucleotides of SEQ ID No: 565 which encodes the amino acid sequence of SEQ ID No: 566. This enzyme is believed to have NAD or NADH binding, NADH dehydrogenase (ubiquinone)
  • Sequence Oxid-284 scaffold00227.G646 (CL07959) is encoded by the nucleotides of SEQ ID No: 567 which encodes the amino acid sequence of SEQ ID No: 568. This enzyme is believed to have sulfite reductase (NADPH)
  • Sequence Oxid-285 scaffold00227.G647 (CL07958) is encoded by the nucleotides of SEQ ID No: 569 which encodes the amino acid sequence of SEQ ID No: 570. This enzyme is believed to have Metal-binding Mitochondrion NAD Oxidoreductase, alcohol dehydrogenase activity.
  • Sequence Oxid-286 scaffbld00071.G964 (CL00580) is encoded by the nucleotides of SEQ ID No: 571 which encodes the amino acid sequence of SEQ ID No: 572. This enzyme is believed to have FMN binding, oxidoreductase activity.
  • Sequence Oxid-287 scaffold00071.G874 (CL00522) is encoded by the nucleotides of SEQ ID No: 573 which encodes the amino acid sequence of SEQ ID No: 574. This enzyme is believed to have fumarate reductase
  • Sequence Oxid-288 scaffold00071.G901 (CL00541) is encoded by the nucleotides of SEQ ID No: 575 which encodes the amino acid sequence of SEQ ID No: 576. This enzyme is believed to have FAD binding, tRNA dihydrouridine synthase activity.
  • Sequence Oxid-289 scaffold00071.G1736 (CL01038) is encoded by the nucleotides of SEQ ID No: 577 which encodes the amino acid sequence of SEQ ID No: 578. This enzyme is believed to have cytochrome-b5 reductase activity.
  • Sequence Oxid-290 scaffold00071.G2980 (CL01805) is encoded by the nucleotides of SEQ ID No: 579 which encodes the amino acid sequence of SEQ ID No: 580. This enzyme is believed to have acyl-CoA dehydrogenase activity, FAD binding activity.
  • Sequence Oxid-291 scaffold00071.G3041 (Cytochrome) is encoded by the nucleotides of SEQ ID No: 581 which encodes the amino acid sequence of SEQ ID No: 582. This enzyme is believed to have D-lactate dehydrogenase activityFAD binding (CL01846) activity.
  • Sequence Oxid-292 scaffold00075.G444 (CL03281) is encoded by the nucleotides of SEQ ID No: 583 which encodes the amino acid sequence of SEQ ID No: 584. This enzyme is believed to have FAD Flavo Protein, electron carrier activity.
  • Sequence Oxid-293 scaffold00075.G498 (CL03310) is encoded by the nucleotides of SEQ ID No: 585 which encodes the amino acid sequence of SEQ ID No: 586. This enzyme is believed to have FAD Flavo Protein, electron carrier activity.
  • Sequence Oxid-294 scaffold00075.G582 (CL03364
  • CL05169 DNA scaffold00075.G582
  • CL03364 CL05169 is encoded by the nucleotides of SEQ ID No: 587 which encodes the amino acid sequence of SEQ ID No: 588.
  • This enzyme is believed to have methylenetetrahydro folate reductase (NADPH) and FAD FlavoProtein NADP Oxidoreductase PhosphoProtein PF02219 1 5 1 20 GO:0005829 GO:0005634 GO:0004489 F:methylenetetrahydrofolate reductase (NADPH) GO:0009086 P:methionine biosynthetic process GO:0055114 P:oxidation reduction activity.
  • NADPH methylenetetrahydro folate reductase
  • FADPH methylenetetrahydrofolate reductase
  • Sequence Oxid-295 scaffold00075.G1087 (CL03644) is encoded by the nucleotides of SEQ ID No: 589 which encodes the amino acid sequence of SEQ ID No: 590. This enzyme is believed to have cytochrome-b5 reductase activity.
  • Sequence Oxid-296 scaffold00122.G187 (CL03910) is encoded by the nucleotides of SEQ ID No: 591 which encodes the amino acid sequence of SEQ ID No: 592. This enzyme is believed to have FAD Flavo Protein, oxidoreductase activity.
  • Sequence Oxid-297 scaffold00131.G377 (CL04457) is encoded by the nucleotides of SEQ ID No: 593 which encodes the amino acid sequence of SEQ ID No: 594. This enzyme is believed to have acyl-CoA dehydrogenase activity.
  • Sequence Oxid-298 scaffold00131.G684 (CL04636) is encoded by the nucleotides of SEQ ID No: 595 which encodes the amino acid sequence of SEQ ID No: 596. This enzyme is believed to have FAD FlavoProtein, oxidoreductase activity.
  • Sequence Oxid-299 scaffold00131.G1217 (CL04976) is encoded by the nucleotides of SEQ ID No: 597 which encodes the amino acid sequence of SEQ ID No: 598. This enzyme is believed to have acyl-CoA dehydrogenase activity, FAD binding activity.
  • Sequence Oxid-300 scaffold00142.G588 (CL08704) is encoded by the nucleotides of SEQ ID No: 599 which encodes the amino acid sequence of 600. This enzyme is believed to have 2 iron, 2 sulfur cluster binding, FAD binding, oxidoreductase activity.
  • Sequence Oxid-301 scaffold00214.G90 (CL02529) is encoded by the nucleotides of SEQ ID No: 601 which encodes the amino acid sequence of SEQ ID No: 602. This enzyme is believed to have FAD binding, NADH dehydrogenase (ubiquinone) activity.
  • Sequence Oxid-302 scaffold00227.G251 (CL08179) is encoded by the nucleotides of SEQ ID No: 603 which encodes the amino acid sequence of SEQ ID No: 604. This enzyme is believed to have 4 iron, 4 sulfur cluster binding, electron-transferring-flavoprotein dehydrogenase activity.
  • Sequence Oxid-303 scaffold00008.Gl (CL11660) is encoded by the nucleotides of SEQ ID No: 605 which encodes the amino acid sequence of SEQ ID No: 606. This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity GO: 0042773 activity.
  • Sequence Oxid-304 scaffold00016.G15 (CL08920) is encoded by the nucleotides of SEQ ID No: 607 which encodes the amino acid sequence of SEQ ID No: 608. This enzyme is believed to have FAD binding, oxidoreductase activity.
  • Sequence Oxid-305 scaffold00016.G18 (CL08922) is encoded by the nucleotides of SEQ ID No: 609 which encodes the amino acid sequence of SEQ ID No: 610.
  • This enzyme is believed to have alcohol dehydrogenase (NADP+) activity, hydroxymethylfurfural reductase (NADH) activity.
  • Sequence Oxid-306 scaffold00016.G58 (CL08949) is encoded by the nucleotides of SEQ ID No: 611 which encodes the amino acid sequence of SEQ ID No: 612. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-307 scaffold00016.Gl 17 (CL08986) is encoded by the nucleotides of SEQ ID No: 613 which encodes the amino acid sequence of SEQ ID No: 614. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-308 scaffold00016.G162 (CL09014) is encoded by the nucleotides of SEQ ID No: 615 which encodes the amino acid sequence of SEQ ID No: 616. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-309 scaffold00016.G280 (CL09100) is encoded by the nucleotides of SEQ ID No: 617 which encodes the amino acid sequence of SEQ ID No: 618. This enzyme is believed to have L-xylulose reductase (NADP+) activity.
  • Sequence Oxid-310 scaffold00016.G288 (CL09105) is encoded by the nucleotides of SEQ ID No: 619 which encodes the amino acid sequence of SEQ ID No: 620. This enzyme is believed to have chorismate synthase activity, oxidoreductase activity.
  • Sequence Oxid-311 30_g scaffold00016.pathl .gene8 is encoded by the nucleotides of SEQ ID No: 621 which encodes the amino acid sequence of SEQ ID No: 622. This enzyme is believed to have
  • NADH hydro xymethylfurfural reductase
  • sequence Oxid-313 359_g scaffold00016.pathl .gene305 scaffold00016.G581 is encoded by the nucleotides of SEQ ID No: 625 which encodes the amino acid sequence of SEQ ID No: 626. This enzyme is believed to have 3-dehydroquinate synthase activity, shikimate 5-dehydrogenase activity.
  • Sequence Oxid-314 393_g is encoded by the nucleotides of SEQ ID No: 627 which encodes the amino acid sequence of SEQ ID No: 628. This enzyme is believed to have glycerol-3-phosphate dehydrogenase activity.
  • sequence Oxid-315 398_g scaffold00016.pathl .gene335 scaffold00016.G648 is encoded by the nucleotides of SEQ ID No: 629 which encodes the amino acid sequence of SEQ ID No: 630. This enzyme is believed to have oxoglutarate dehydrogenase succinyl-trans activity.
  • Sequence Oxid-316 438_g scaffold00016.pathl .gene371 scaffold00016.G695 is encoded by the nucleotides of SEQ ID No: 631 which encodes the amino acid sequence of SEQ ID No: 632. This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity.
  • sequence Oxid-317 474_g scaffold00016.pathl .gene402 scaffold00016.G749 is encoded by the nucleotides of SEQ ID No: 633 which encodes the amino acid sequence of SEQ ID No: 634. This enzyme is believed to have alcohol dehydrogenase (NAD) activity.
  • NAD alcohol dehydrogenase
  • the enzyme identified as Sequence Oxid-3118 632_g scaflbld00016.pathl .gene545 scaffold00016.G996 is encoded by the nucleotides of SEQ ID No: 635 which encodes the amino acid sequence of SEQ ID No: 636. This enzyme is believed to have glyceraldehyde-3-phosphate dehydrogenase activity.
  • Sequence Oxid-320 718_g scaffold00016.pathl .gene617 is encoded by the nucleotides of SEQ ID No: 639 which encodes the amino acid sequence of SEQ ID No: 640. This enzyme is believed to have xanthine dehydrogenase activity.
  • sequence Oxid-321 789_g scaffold00016.pathl .gene675 scaffold00016.G1249 is encoded by the nucleotides of SEQ ID No: 641 which encodes the amino acid sequence of SEQ ID No: 642. This enzyme is believed to have aldehyde dehydrogenase (NAD) activity.
  • NAD aldehyde dehydrogenase
  • Sequence Oxid-322 1037_g is encoded by the nucleotides of SEQ ID No: 643 which encodes the amino acid sequence of SEQ ID No: 644.
  • This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity.
  • the enzyme identified as Sequence Oxid-323 1186_g scaffold00031.pathl .gene222 scaffold00031.G414 is encoded by the nucleotides of SEQ ID No: 645 which encodes the amino acid sequence of SEQ ID No: 646. This enzyme is believed to have L-malate dehydrogenase activity.
  • Sequence Oxid-324 1292_g scaffold00031.pathl .gene320 is encoded by the nucleotides of SEQ ID No: 647 which encodes the amino acid sequence of SEQ ID No: 648. This enzyme is believed to have D-lactate dehydrogenase (Cytochrome) activity.
  • sequence Oxid-325 1294_g scaffold00031.pathl .gene322 scaffold00031.G568 is encoded by the nucleotides of SEQ ID No: 649 which encodes the amino acid sequence of SEQ ID No: 650. This enzyme is believed to have pyruvate dehydrogenase acetyl-transferring activity.
  • Sequence Oxid-326 1412_g scaffold00031.G751 is encoded by the nucleotides of SEQ ID No: 651 which encodes the amino acid sequence of SEQ ID No: 652. This enzyme is believed to have 3-isopropylmalate dehydrogenase activity.
  • Sequence Oxid-327 1474_g scaffold00031.pathl .gene483 is encoded by the nucleotides of SEQ ID No: 653 which encodes the amino acid sequence of SEQ ID No: 654. This enzyme is believed to have NADH
  • ubiquinone dehydrogenase
  • Sequence Oxid-328 1589_g scaffold00031.pathl .gene571 is encoded by the nucleotides of SEQ ID No: 655 which encodes the amino acid sequence of SEQ ID No: 656. This enzyme is believed to have NADH
  • ubiquinone dehydrogenase
  • Sequence Oxid-329 1606_g scaffold00031.pathl .gene587 is encoded by the nucleotides of SEQ ID No: 657 which encodes the amino acid sequence of SEQ ID No: 658. This enzyme is believed to have prephenate dehydrogenase (NADP+) activity.
  • Sequence Oxid-330 161 l_g is encoded by the nucleotides of SEQ ID No: 659 which encodes the amino acid sequence of SEQ ID No: 660.
  • This enzyme is believed to have glutamate dehydrogenase activity.
  • Sequence Oxid-331 1694_g scaffold00031.pathl .gene670 is encoded by the nucleotides of SEQ ID No: 661 which encodes the amino acid sequence of SEQ ID No: 662. This enzyme is believed to have NADPH dehydrogenase activity.
  • Sequence Oxid-332 1753_g scaffold00031.pathl .gene729 scaffold00031.G1281 is encoded by the nucleotides of SEQ ID No: 663 which encodes the amino acid sequence of SEQ ID No: 664. This enzyme is believed to have 3-hydroxyisobutyrate dehydrogenase activity.
  • sequence Oxid-333 1779_g scaffold00031.pathl .gene754 is encoded by the nucleotides of SEQ ID No: 665 which encodes the amino acid sequence of SEQ ID No: 666. This enzyme is believed to have acyl-CoA dehydrogenase activity.
  • Sequence Oxid-334 1822_g scaffold00031.pathl .gene786 is encoded by the nucleotides of SEQ ID No: 667 which encodes the amino acid sequence of SEQ ID No: 668. This enzyme is believed to have dihydroorotate dehydrogenase activity.
  • the enzyme identified as Sequence Oxid-335 1855_g scaflbld00031.pathl .gene813 scaffold00031.G1450 is encoded by the nucleotides of SEQ ID No: 669 which encodes the amino acid sequence of SEQ ID No: 670. This enzyme is believed to have mannitol-1 -phosphate 5-dehydrogenase activity.
  • sequence Oxid-336 2041_g scaffold00031.pathl .gene953 scaffold00031.G1782 is encoded by the nucleotides of SEQ ID No: 671 which encodes the amino acid sequence of SEQ ID No: 672. This enzyme is believed to have L-iditol 2-dehydrogenase activity.
  • sequence Oxid-337 2115_g scaffold00050.pathl .genel2 is encoded by the nucleotides of SEQ ID No: 673 which encodes the amino acid sequence of SEQ ID No: 674. This enzyme is believed to have proline
  • sequence Oxid-339 2678_g scaffold00050.pathl .gene468 scaffold00050.G966 is encoded by the nucleotides of SEQ ID No: 677 which encodes the amino acid sequence of SEQ ID No: 678. This enzyme is believed to have acyl-CoA dehydrogenase activity.
  • Sequence Oxid-340 2682_g scaffold00050.pathl .gene472 is encoded by the nucleotides of SEQ ID No: 679 which encodes the amino acid sequence of SEQ ID No: 680. This enzyme is believed to have acyl-CoA dehydrogenase activity.
  • sequence Oxid-341 2813_g scaffold00050.pathl .gene585 scaffold00050.Gl 184 is encoded by the nucleotides of SEQ ID No: 681 which encodes the amino acid sequence of SEQ ID No: 682. This enzyme is believed to have quinate 5-dehydrogenase activity.
  • Sequence Oxid-342 2879_g scaffold00050.pathl .gene643 is encoded by the nucleotides of SEQ ID No: 683 which encodes the amino acid sequence of SEQ ID No: 684. This enzyme is believed to have dihydrolipoyl dehydrogenase activity.
  • Sequence Oxid-343 3035_g is encoded by the nucleotides of SEQ ID No: 685 which encodes the amino acid sequence of SEQ ID No: 686. This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity.
  • Sequence Oxid-344 3108_g scaffold00050.G1648 is encoded by the nucleotides of SEQ ID No: 687 which encodes the amino acid sequence of SEQ ID No: 688. This enzyme is believed to have NADH
  • ubiquinone dehydrogenase
  • sequence Oxid-345 3228_g scaffold00065.pathl .genel scaffold00065.Gl is encoded by the nucleotides of SEQ ID No: 689 which encodes the amino acid sequence of SEQ ID No: 690. This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity.
  • sequence Oxid-346 3253_g scaffold00071.pathl .genel0 scaffold00071.G21 is encoded by the nucleotides of SEQ ID No: 691 which encodes the amino acid sequence of SEQ ID No: 692. This enzyme is believed to have L-iditol 2-dehydrogenase activity.
  • Sequence Oxid-347 3282_g is encoded by the nucleotides of SEQ ID No: 693 which encodes the amino acid sequence of SEQ ID No: 694. This enzyme is believed to have D-lactate dehydrogenase (Cytochrome) activity.
  • sequence Oxid-348 3303_g scaffold00071.pathl .gene52 scaffold00071.G104 is encoded by the nucleotides of SEQ ID No: 695 which encodes the amino acid sequence of SEQ ID No: 696. This enzyme is believed to have succinate-semialdehyde dehydrogenase [NAD(P activity.
  • the enzyme identified as Sequence Oxid-349 343 l_g scaffold00071.pathl .genel65 scaffold00071.G332 is encoded by the nucleotides of SEQ ID No: 697 which encodes the amino acid sequence of SEQ ID No: 698. This enzyme is believed to have D-arabinitol 2-dehydrogenase activity.
  • sequence Oxid-350 3546_g scaffold00071.pathl .gene254 scaffold00071.G527 is encoded by the nucleotides of SEQ ID No: 699 which encodes the amino acid sequence of 700. This enzyme is believed to have L-iditol 2-dehydrogenase activity.
  • the enzyme identified as Sequence Oxid-351 3585_g scaffold00071.pathl .gene293 scaffold00071.G593 is encoded by the nucleotides of SEQ ID No: 701 which encodes the amino acid sequence of SEQ ID No: 702. This enzyme is believed to have succinate dehydrogenase (ubiquinone) activity.
  • the enzyme identified as Sequence Oxid-352 3624_g scaflbld00071.pathl .gene325 scaffold00071.G644 is encoded by the nucleotides of SEQ ID No: 703 which encodes the amino acid sequence of SEQ ID No: 704. This enzyme is believed to have glucose-6-phosphate dehydrogenase activity.
  • sequence Oxid-353 3725_g scaffold00071.pathl .gene419 scaffold00071.G784 is encoded by the nucleotides of SEQ ID No: 705 which encodes the amino acid sequence of SEQ ID No: 706. This enzyme is believed to have aldehyde dehydrogenase (NADP+) activity.
  • Sequence Oxid-354 3788_g scaffold00071.pathl .gene475 is encoded by the nucleotides of SEQ ID No: 707 which encodes the amino acid sequence of SEQ ID No: 708. This enzyme is believed to have succinate dehydrogenase activity.
  • sequence Oxid-355 3813_g scaffold00071.pathl .gene498 scaffold00071.G905 is encoded by the nucleotides of SEQ ID No: 709 which encodes the amino acid sequence of SEQ ID No: 710. This enzyme is believed to have succinate dehydrogenase (ubiquinone) activity.
  • sequence Oxid-356 3849_g scaffold00071.pathl .gene525 scaffold00071.G953 is encoded by the nucleotides of SEQ ID No : 711 which encodes the amino acid sequence of SEQ ID No: 712. This enzyme is believed to have homoisocitrate dehydrogenase activity.
  • the enzyme identified as Sequence Oxid-357 3893_g scaffold00071.pathl .gene569 scaffold00071.G1018 is encoded by the nucleotides of SEQ ID No: 713 which encodes the amino acid sequence of SEQ ID No: 714. This enzyme is believed to have L-aminoadipate-semialdehyde dehydrogenase activity.
  • Sequence Oxid-358 3904_g scaffold00071.G1033 is encoded by the nucleotides of SEQ ID No: 715 which encodes the amino acid sequence of SEQ ID No: 716. This enzyme is believed to have aldehyde dehydrogenase (NAD) activity.
  • NAD aldehyde dehydrogenase
  • the enzyme identified as Sequence Oxid-359 3907_g scaffold00071.pathl .gene584 scaffold00071.G1037 is encoded by the nucleotides of SEQ ID No: 717 which encodes the amino acid sequence of SEQ ID No: 718. This enzyme is believed to have L-lactate dehydrogenase activity.
  • the enzyme identified as Sequence Oxid-360 3973_g scaflbld00071.pathl .gene627 scaffold00071.Gl 160 is encoded by the nucleotides of SEQ ID No: 719 which encodes the amino acid sequence of SEQ ID No: 720. This enzyme is believed to have iso citrate dehydrogenase (NAD+) activity.
  • sequence Oxid-361 4020_g scaffold00071.pathl .gene663 scaffold00071.G1266 is encoded by the nucleotides of SEQ ID No: 721 which encodes the amino acid sequence of SEQ ID No: 722. This enzyme is believed to have glycerol-3-phosphate dehydrogenase (NAD+) activity.
  • sequence Oxid-362 4078_g scaffold00071.pathl .gene715 scaffold00071.G1360 is encoded by the nucleotides of SEQ ID No: 723 which encodes the amino acid sequence of SEQ ID No: 724. This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity.
  • Sequence Oxid-363 4133_g scaffold00071.pathl .gene764 is encoded by the nucleotides of SEQ ID No: 725 which encodes the amino acid sequence of SEQ ID No: 726. This enzyme is believed to have NADPH dehydrogenase activity.
  • the enzyme identified as Sequence Oxid-364 4146_g scaffold00071.pathl .gene776 scaffold00071.G1457 is encoded by the nucleotides of SEQ ID No: 727 which encodes the amino acid sequence of SEQ ID No: 728. This enzyme is believed to have 3 -beta-hydro xy-delta5 -steroid dehydrogenase activity.
  • sequence Oxid-365 4158_g scaffold00071.pathl .gene786 scaffold00071.G1476 is encoded by the nucleotides of SEQ ID No: 729 which encodes the amino acid sequence of SEQ ID No: 730. This enzyme is believed to have succinate-semialdehyde dehydrogenase activity.
  • sequence Oxid-366 4163_g scaffold00071.pathl .gene791 scaffold00071.G1485 is encoded by the nucleotides of SEQ ID No: 731 which encodes the amino acid sequence of SEQ ID No: 732. This enzyme is believed to have pyridoxine 4-dehydrogenase activity.
  • the enzyme identified as Sequence Oxid-367 4300_g scaffold00071.pathl .gene902 scaffold00071.G1693 is encoded by the nucleotides of SEQ ID No: 733 which encodes the amino acid sequence of SEQ ID No: 734. This enzyme is believed to have histidinol dehydrogenase activity, phosphoribosyl-AMP cyclohydrolase activity.
  • the enzyme identified as Sequence Oxid-368 4326_g scaflbld00071.pathl .gene926 scaffold00071.G1722 is encoded by the nucleotides of SEQ ID No: 735 which encodes the amino acid sequence of SEQ ID No: 736. This enzyme is believed to have saccharopine dehydrogenase (NAD+, L-lysine- activity.
  • the enzyme identified as Sequence Oxid-369 4327_g scaffold00071.pathl .gene927 scaffold00071.G1723 is encoded by the nucleotides of SEQ ID No: 737 which encodes the amino acid sequence of SEQ ID No: 738. This enzyme is believed to have IMP dehydrogenase activity.
  • Sequence Oxid-370 4345_g is encoded by the nucleotides of SEQ ID No: 739 which encodes the amino acid sequence of SEQ ID No: 740. This enzyme is believed to have phospho gluconate dehydrogenase (decarboxylase) activity.
  • sequence Oxid-371 4370_g scaffold00071.pathl .gene967 scaffold00071.G1784 is encoded by the nucleotides of SEQ ID No: 741 which encodes the amino acid sequence of SEQ ID No: 742. This enzyme is believed to have l-pyrroline-5-carboxylate dehydrogenase activity.
  • scaffold00071.pathl .genel l33 scaffold00071.G2097 is encoded by the nucleotides of SEQ ID No: 743 which encodes the amino acid sequence of SEQ ID No: 744.
  • This enzyme is believed to have 3 -beta-hydro xy-delta5 -steroid dehydrogenase, sterol-4-alpha-carboxylate 3-dehydrogenase activity.
  • scaffold00071.pathl .genel l68 scaffold00071.G2157 is encoded by the nucleotides of SEQ ID No: 745 which encodes the amino acid sequence of SEQ ID No: 746. This enzyme is believed to have methylenetetrahydro folate dehydrogenase activity.
  • scaffold00071.pathl .genel276 is encoded by the nucleotides of SEQ ID No: 747 which encodes the amino acid sequence of SEQ ID No: 748. This enzyme is believed to have L-lactate dehydrogenase (Cytochrome) activity.
  • scaffold00071.pathl .genel294 scaffold00071.G2411 is encoded by the nucleotides of SEQ ID No: 749 which encodes the amino acid sequence of SEQ ID No: 750. This enzyme is believed to have 3 -beta-hydro xy-delta5 -steroid dehydrogenase activity.
  • scaffold00071.pathl .genel357 scaffold00071.G2544 is encoded by the nucleotides of SEQ ID No: 751 which encodes the amino acid sequence of SEQ ID No: 752. This enzyme is believed to have pyruvate dehydrogenase (acetyl-transferring) activity.
  • scaffold00071.pathl .genel439 scaffold00071.G2698 is encoded by the nucleotides of SEQ ID No: 753 which encodes the amino acid sequence of SEQ ID No: 754.
  • This enzyme is believed to have aspartate-semialdehyde dehydrogenase activity.
  • Sequence Oxid-378 5088_g scaffold00071.G2977 is encoded by the nucleotides of SEQ ID No: 755 which encodes the amino acid sequence of SEQ ID No: 756. This enzyme is believed to have D-arabinitol 2- dehydrogenase activity.
  • scaffold00071.pathl .genel581 is encoded by the nucleotides of SEQ ID No: 757 which encodes the amino acid sequence of SEQ ID No: 758. This enzyme is believed to have acyl-CoA dehydrogenase activity.
  • Sequence Oxid-380 513 l_g is encoded by the nucleotides of SEQ ID No: 759 which encodes the amino acid sequence of SEQ ID No: 760.
  • This enzyme is believed to have D-lactate dehydrogenase (Cytochrome) activity.
  • scaffold00071.pathl .genel777 scaffold00071.G3328 is encoded by the nucleotides of SEQ ID No: 761 which encodes the amino acid sequence of SEQ ID No: 762.
  • This enzyme is believed to have aldehyde reductase activity, glucose 1- dehydrogenase (NADP+) activity.
  • scaffold00071.pathl .genel843 scaffold00071.G3467 is encoded by the nucleotides of SEQ ID No: 763 which encodes the amino acid sequence of SEQ ID No: 764.
  • This enzyme is believed to have aldehyde dehydrogenase (NAD) activity.
  • scaffold00071.pathl .genel862 scaffold00071.G3501 is encoded by the nucleotides of SEQ ID No: 765 which encodes the amino acid sequence of SEQ ID No: 766. This enzyme is believed to have L-lactate dehydrogenase activity.
  • scaffold00071.pathl .genel869 scaffold00071.G3512 is encoded by the nucleotides of SEQ ID No: 767 which encodes the amino acid sequence of SEQ ID No: 768. This enzyme is believed to have glycine dehydrogenase (decarboxylating) activity.
  • the enzyme identified as Sequence Oxid-385 5625_g scaffold00075.pathl .genel71 scaffold00075.G374 is encoded by the nucleotides of SEQ ID No: 769 which encodes the amino acid sequence of SEQ ID No: 770. This enzyme is believed to have 3-hydroxyisobutyrate dehydrogenase activity, histone-lysine N- methyltransferase activity,phospho gluconate dehydrogenase (decarboxylase) activity.
  • sequence Oxid-386 5768_g scaffold00075.pathl .gene311 is encoded by the nucleotides of SEQ ID No: 771 which encodes the amino acid sequence of SEQ ID No: 772. This enzyme is believed to have isocitrate dehydrogenase (NAD+) activity.
  • sequence Oxid-387 5850_g scaffold00075.pathl .gene393 scaffold00075.G724 is encoded by the nucleotides of SEQ ID No: 773 which encodes the amino acid sequence of SEQ ID No: 774. This enzyme is believed to have phospho gluconate dehydrogenase (decarboxylase) activity.
  • Sequence Oxid-388 5919_g is encoded by the nucleotides of SEQ ID No: 775 which encodes the amino acid sequence of SEQ ID No: 776. This enzyme is believed to have L-lactate dehydrogenase (Cytochrome) activity.
  • the enzyme identified as Sequence Oxid-389 5937_g scaffold00075.pathl .gene469 is encoded by the nucleotides of SEQ ID No: 777 which encodes the amino acid sequence of SEQ ID No: 778. This enzyme is believed to have 3-dehydroquinate dehydratase activity, shikimate 5-dehydrogenase activity, shikimate kinase activity.
  • the enzyme identified as Sequence Oxid-390 613 l_g scaffold00075.pathl .gene641 scaffold00075.Gl 167 is encoded by the nucleotides of SEQ ID No: 779 which encodes the amino acid sequence of SEQ ID No: 780. This enzyme is believed to have betaine-aldehyde dehydrogenase activity.
  • the enzyme identified as Sequence Oxid-391 6269_g scaffold00092.pathl .gene24 scaffold00092.G43 is encoded by the nucleotides of SEQ ID No: 781 which encodes the amino acid sequence of SEQ ID No: 782. This enzyme is believed to have precorrin-2 dehydrogenase activity,F:sirohydrochlorin ferrochelatase activity.
  • the enzyme identified as Sequence Oxid-392 6348_g scaffold00092.pathl .gene81 scaffold00092.G162 is encoded by the nucleotides of SEQ ID No: 783 which encodes the amino acid sequence of SEQ ID No: 784. This enzyme is believed to have L-malate dehydrogenase activity.
  • Sequence Oxid-393 6537_g scaffold00092.pathl .gene235 is encoded by the nucleotides of SEQ ID No: 785 which encodes the amino acid sequence of SEQ ID No: 786. This enzyme is believed to have cellobiose dehydrogenase (acceptor) activity.
  • sequence Oxid-394 6692_g scaffold00092.pathl .gene387 scaffold00092.G738 is encoded by the nucleotides of SEQ ID No: 787 which encodes the amino acid sequence of SEQ ID No: 788. This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity.
  • sequence Oxid-395 6950_g scaffold00122.pathl .genel07 scaffold00122.G197 is encoded by the nucleotides of SEQ ID No: 789 which encodes the amino acid sequence of SEQ ID No: 790. This enzyme is believed to have aldehyde dehydrogenase (NAD) activity.
  • NAD aldehyde dehydrogenase
  • ubiquinone dehydrogenase
  • Sequence Oxid-397 7048_g scaffold00131.pathl .gene70 scaffold00131.G132 is encoded by the nucleotides of SEQ ID No: 793 which encodes the amino acid sequence of SEQ ID No: 794. This enzyme is believed to have alcohol dehydrogenase (NADP+) activity.
  • sequence Oxid-398 7101_g scaffold00131.pathl .genel20 scaffold00131.G220 is encoded by the nucleotides of SEQ ID No: 795 which encodes the amino acid sequence of SEQ ID No: 796. This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity.
  • the enzyme identified as Sequence Oxid-399 7117_g scaffold00131.pathl .genel32 scaffold00131.G239 is encoded by the nucleotides of SEQ ID No: 797 which encodes the amino acid sequence of SEQ ID No: SEQ ID No: 798. This enzyme is believed to have acyl-CoA dehydrogenase activity.
  • the enzyme identified as Sequence Oxid-400 7135_g scaflbld00131.pathl .genel50 scaffold00131.G266 is encoded by the nucleotides of SEQ ID No: 799 which encodes the amino acid sequence of 800. This enzyme is believed to have 3- dehydroquinate synthase activity, 3-phosphoshikimate 1- carboxyvinyltransferas,shikimate 5-dehydrogenase activity, shikimate kinase activity.
  • Sequence Oxid-401 7136_g scaffold00131.pathl .genel52 is encoded by the nucleotides of SEQ ID No: 801 which encodes the amino acid sequence of SEQ ID No: 802. This enzyme is believed to have dihydroorotate dehydrogenase activity.
  • Sequence Oxid-402 7207_g scaffold00131.pathl .gene222 is encoded by the nucleotides of SEQ ID No: 803 which encodes the amino acid sequence of SEQ ID No: 804. This enzyme is believed to have acyl-CoA dehydrogenase activity.
  • sequence Oxid-403 7244_g scaffold00131.pathl .gene256 scaffold00131.G441 is encoded by the nucleotides of SEQ ID No: 805 which encodes the amino acid sequence of SEQ ID No: 806. This enzyme is believed to have malate dehydrogenase (oxaloacetate-decarboxylase) activity.
  • sequence Oxid-404 7344_g scaffold00131.pathl .gene349 scaffold00131.G586 is encoded by the nucleotides of SEQ ID No: 807 which encodes the amino acid sequence of SEQ ID No: 808. This enzyme is believed to have pyruvate dehydrogenase (acetyl-transferring GO:0015976 activity.
  • sequence Oxid-405 7359_g scaffold00131.pathl .gene365 is encoded by the nucleotides of SEQ ID No: 809 which encodes the amino acid sequence of SEQ ID No: 810. This enzyme is believed to have oxidoreductase activity, acting on the CH-0 activity.
  • Sequence Oxid-406 7681_g scaffold00131.Gl 152 is encoded by the nucleotides of SEQ ID No : 811 which encodes the amino acid sequence of SEQ ID No: 812. This enzyme is believed to have succinate dehydrogenase activity.
  • sequence Oxid-407 7688_g scaffold00131.pathl .gene645 scaffold00131.G1161 is encoded by the nucleotides of SEQ ID No: 813 which encodes the amino acid sequence of SEQ ID No: 814. This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity.
  • the enzyme identified as Sequence Oxid-408 7715_g scaflbld00131.pathl .gene672 scaffold00131.G1199 is encoded by the nucleotides of SEQ ID No: 815 which encodes the amino acid sequence of SEQ ID No: 816. This enzyme is believed to have glutamate dehydrogenase (NADP+) activity.
  • sequence Oxid-409 7732_g scaffold00131.pathl .gene687 is encoded by the nucleotides of SEQ ID No: 817 which encodes the amino acid sequence of SEQ ID No: 818. This enzyme is believed to have acyl-CoA dehydrogenase activity.
  • sequence Oxid-410 7808_g scaffold00131.pathl .gene747 scaffold00131.G1330 is encoded by the nucleotides of SEQ ID No: 819 which encodes the amino acid sequence of SEQ ID No: 820. This enzyme is believed to have phosphoglycerate dehydrogenase activity.
  • scaffold00131.pathl .gene895 is encoded by the nucleotides of SEQ ID No: 821 which encodes the amino acid sequence of SEQ ID No: 822. This enzyme is believed to have saccharopine dehydrogenase (NADP+, L-glutamase) activity.
  • scaffold00131.pathl .genel039 scaffold00131.G1851 is encoded by the nucleotides of SEQ ID No: 823 which encodes the amino acid sequence of SEQ ID No: 824. This enzyme is believed to have S-(hydroxymethyl)glutathione dehydrogenase activity.
  • scaffold00131.pathl .genel073 is encoded by the nucleotides of SEQ ID No: 825 which encodes the amino acid sequence of SEQ ID No: 826. This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity.
  • Sequence Oxid-414 8429_g scaffold00137.pathl .gene47 is encoded by the nucleotides of SEQ ID No: 827 which encodes the amino acid sequence of SEQ ID No: 828. This enzyme is believed to have carbohydrate binding, cellobiose dehydrogenase (acceptor) activity.
  • Sequence Oxid-415 8601_g scaffold00142.pathl .gene76 scaffold00142.G177 is encoded by the nucleotides of SEQ ID No: 829 which encodes the amino acid sequence of SEQ ID No: 830. This enzyme is believed to have iso citrate dehydrogenase (NADP+) activity.
  • scaffold00142.pathl .gene91 is encoded by the nucleotides of SEQ ID No: 831 which encodes the amino acid sequence of SEQ ID No: 832. This enzyme is believed to have methylenetetrahydro folate dehydrogenase activity.
  • scaffold00142.G217 scaffold00142.pathl .genel01, scaffold00142.pathl .genel02 is encoded by the nucleotides of SEQ ID No: 833 which encodes the amino acid sequence of SEQ ID No: 834. This enzyme is believed to have NADH
  • ubiquinone dehydrogenase
  • scaffold00142.pathl .genel09 is encoded by the nucleotides of SEQ ID No: 835 which encodes the amino acid sequence of SEQ ID No: 836. This enzyme is believed to have aldehyde dehydrogenase [NAD(P)+] activity.
  • scaffold00142.pathl .genel36 is encoded by the nucleotides of SEQ ID No: 837 which encodes the amino acid sequence of SEQ ID No: 838. This enzyme is believed to have cellobiose dehydrogenase (acceptor) activity.
  • scaffold00142.patnl .genel72 is encoded by the nucleotides of SEQ ID No: 839 which encodes the amino acid sequence of SEQ ID No: 840. This enzyme is believed to have phospho gluconate dehydrogenase (decarboxylase) activity.
  • scaffold00169.pathl .genel43 is encoded by the nucleotides of SEQ ID No: 841 which encodes the amino acid sequence of SEQ ID No: 842. This enzyme is believed to have alcohol dehydrogenase (NADP+) activity, hydroxymethylfurfural reductase (NADH) activity.
  • NADP+ alcohol dehydrogenase
  • NADH hydroxymethylfurfural reductase
  • NADP+ dehydrogenase
  • NADH hydroxymethylfurfural reductase
  • sequence Oxid-423 9103_g scaffold00169.G346 scaffold00169.pathl .genel70 is encoded by the nucleotides of SEQ ID No: 845 which encodes the amino acid sequence of SEQ ID No: 846. This enzyme is believed to have acyl-CoA dehydrogenase activity.
  • scaffold00214.pathl .gene51 is encoded by the nucleotides of SEQ ID No: 847 which encodes the amino acid sequence of SEQ ID No: 848. This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity.
  • scaffold00227.pathl .gene78 is encoded by the nucleotides of SEQ ID No: 849 which encodes the amino acid sequence of SEQ ID No: 850. This enzyme is believed to have phosphoglycerate dehydrogenase activity.
  • scaffold00227.pathl .gene80 is encoded by the nucleotides of SEQ ID No: 851 which encodes the amino acid sequence of SEQ ID No: 852. This enzyme is believed to have phosphoglycerate dehydrogenase activity.
  • scaffold00227.pathl .gene204 is encoded by the nucleotides of SEQ ID No: 853 which encodes the amino acid sequence of SEQ ID No: 854. This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity.
  • scaffold00227.pathl .gene303 is encoded by the nucleotides of SEQ ID No: 855 which encodes the amino acid sequence of SEQ ID No: 856. This enzyme is believed to have homo serine dehydrogenase activity.
  • scaffold00227.pathl .gene360 is encoded by the nucleotides of SEQ ID No: 857 which encodes the amino acid sequence of SEQ ID No: 858. This enzyme is believed to have NADH dehydrogenase (ubiquinone) activity.
  • the enzyme identified as Sequence Oxid-430 8277_g scaffold00131.G2107 scaffold00131.pathl .genel l68 is encoded by the nucleotides of SEQ ID No: 859 which encodes the amino acid sequence of SEQ ID No: 860. This enzyme is believed to have glutamate-5-semialdehyde dehydrogenase activity.
  • scaffold00142.pathl .genel80 is encoded by the nucleotides of SEQ ID No: 861 which encodes the amino acid sequence of SEQ ID No: 862. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-432 8723_g scaffold00142.pathl .genel81 is encoded by the nucleotides of SEQ ID No: 863 which encodes the amino acid sequence of SEQ ID No: 864. This enzyme is believed to have flavin-containing monooxygenase activity.
  • scaffold00142.pathl .gene280 is encoded by the nucleotides of SEQ ID No: 865 which encodes the amino acid sequence of SEQ ID No: 866. This enzyme is believed to have monooxygenase activity.
  • scaffold00142.pathl .gene280, scaffold00142.pathl .gene281 is encoded by the nucleotides of SEQ ID No: 867 which encodes the amino acid sequence of SEQ ID No: 868. This enzyme is believed to have monooxygenase activity.
  • scaffold00142.pathl .gene289 is encoded by the nucleotides of SEQ ID No: 869 which encodes the amino acid sequence of SEQ ID No: 870. This enzyme is believed to have monooxygenase activity.
  • scaffold00169.pathl .genel6 is encoded by the nucleotides of SEQ ID No: 871 which encodes the amino acid sequence of SEQ ID No: 872. This enzyme is believed to have monooxygenase activity.
  • scaffold00169.pathl .gene20 is encoded by the nucleotides of SEQ ID No: 873 which encodes the amino acid sequence of SEQ ID No: 874. This enzyme is believed to have monooxygenase activity.
  • scaffold00169.pathl .genel37 is encoded by the nucleotides of SEQ ID No: 875 which encodes the amino acid sequence of SEQ ID No: 876. This enzyme is believed to have monooxygenase activity.
  • scaffold00169.pathl .genel58 is encoded by the nucleotides of SEQ ID No: 877 which encodes the amino acid sequence of SEQ ID No: 878. This enzyme is believed to have flavin-containing monooxygenase activity.
  • scaffold00169.G318 is encoded by the nucleotides of SEQ ID No: 879 which encodes the amino acid sequence of SEQ ID No: 880. This enzyme is believed to have flavin-containing monooxygenase activity.
  • Sequence Oxid-441 9087_g is encoded by the nucleotides of SEQ ID No: 881 which encodes the amino acid sequence of SEQ ID No: 882.
  • This enzyme is believed to have flavin-containing monooxygenase activity.
  • scaffold00169.pathl .genel61 is encoded by the nucleotides of SEQ ID No: 883 which encodes the amino acid sequence of SEQ ID No: 884. This enzyme is believed to have monooxygenase activity.
  • scaffold00169.pathl .genel62 is encoded by the nucleotides of SEQ ID No: 885 which encodes the amino acid sequence of SEQ ID No: 886. This enzyme is believed to have flavin-containing monooxygenase activity.
  • scaffold00169.pathl .genel95 is encoded by the nucleotides of SEQ ID No: 887 which encodes the amino acid sequence of SEQ ID No: 888. This enzyme is believed to have monooxygenase activity.
  • scaffold00169.pathl .gene209 is encoded by the nucleotides of SEQ ID No: 889 which encodes the amino acid sequence of SEQ ID No: 890. This enzyme is believed to have monooxygenase activity.
  • scaffold00214.G10 scaffold00214.Gl 1 scafGerman00214.pathl .gene7,
  • scaffblg00214.pathl .gene8 is encoded by the nucleotides of SEQ ID No: 893 which encodes the amino acid sequence of SEQ ID No: 894. This enzyme is believed to have monooxygenase activity.
  • the enzyme identified as Sequence Oxid-448 9315_g scaffold00214.G22 scaffold00214.pathl .genel6 is encoded by the nucleotides of SEQ ID No: 895 which encodes the amino acid sequence of SEQ ID No: 896. This enzyme is believed to have monooxygenase activity.
  • scaffold00227.pathl .gene52 is encoded by the nucleotides of SEQ ID No: 897 which encodes the amino acid sequence of SEQ ID No: 898. This enzyme is believed to have monooxygenase activity.
  • scaffold00227.pathl .genel06 is encoded by the nucleotides of SEQ ID No: 899 which encodes the amino acid sequence of 900. This enzyme is believed to have monooxygenase activity.
  • scaffold00227.pathl .gene224 is encoded by the nucleotides of SEQ ID No: 901 which encodes the amino acid sequence of SEQ ID No: 902. This enzyme is believed to have ent-kaurene oxidase activity.
  • scaffold00227.pathl .gene379 is encoded by the nucleotides of SEQ ID No: 903 which encodes the amino acid sequence of SEQ ID No: 904. This enzyme is believed to have monooxygenase activity.
  • scaffold00016.pathl .genel75 is encoded by the nucleotides of SEQ ID No: 905 which encodes the amino acid sequence of SEQ ID No: 906. This enzyme is believed to have phenol 2-monooxygenase activity.
  • Sequence Oxid-454 671_g (CL09554) is encoded by the nucleotides of SEQ ID No: 907 which encodes the amino acid sequence of SEQ ID No: 908. This enzyme is believed to have monooxygenase activity.
  • scaffold00019.pathl .gene7 is encoded by the nucleotides of SEQ ID No: 909 which encodes the amino acid sequence of SEQ ID No: 910. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-456 887_g is encoded by the nucleotides of SEQ ID No: 911 which encodes the amino acid sequence of SEQ ID No: 912. This enzyme is believed to have monooxygenase activity.
  • scaffbld00019.pathl .genel5 is encoded by the nucleotides of SEQ ID No: 913 which encodes the amino acid sequence of SEQ ID No: 914. This enzyme is believed to have monooxygenase activity.
  • scaffold00031.G18, scaffold00031.pathl .genel4, scaffold00031.pathl .genel5 is encoded by the nucleotides of SEQ ID No: 915 which encodes the amino acid sequence of SEQ ID No: 916. This enzyme is believed to have monooxygenase activity.
  • scaffold00031.pathl .gene42, scaffold00031.pathl .gene43 is encoded by the nucleotides of SEQ ID No: 917 which encodes the amino acid sequence of SEQ ID No: 918. This enzyme is believed to have phenol 2-monooxygenase activity.
  • scaffold00031.pathl .gene69 is encoded by the nucleotides of SEQ ID No: 919 which encodes the amino acid sequence of SEQ ID No: 920. This enzyme is believed to have monooxygenase activity.
  • scaffold00031.pathl .genel 15 is encoded by the nucleotides of SEQ ID No: 921 which encodes the amino acid sequence of SEQ ID No: 922. This enzyme is believed to have flavin-containing monooxygenase activity.
  • pathl . gene252 is encoded by the nucleotides of SEQ ID No: 923 which encodes the amino acid sequence of SEQ ID No: 924. This enzyme is believed to have monooxygenase activity.
  • pathl . gene263 is encoded by the nucleotides of SEQ ID No: 925 which encodes the amino acid sequence of SEQ ID No: 926. This enzyme is believed to have monooxygenase activity.
  • pathl . gene521 is encoded by the nucleotides of SEQ ID No: 927 which encodes the amino acid sequence of SEQ ID No: 928. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-465 1643_g scaffold00031.Gl 133 is encoded by the nucleotides of SEQ ID No: 929 which encodes the amino acid sequence of SEQ ID No: 930. This enzyme is believed to have monooxygenase activity.
  • scaffold00031.pathl .gene780 is encoded by the nucleotides of SEQ ID No: 931 which encodes the amino acid sequence of SEQ ID No: 932. This enzyme is believed to have monooxygenase activity.
  • scaffold00031.pathl .gene781 is encoded by the nucleotides of SEQ ID No: 933 which encodes the amino acid sequence of SEQ ID No: 934. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-468 1820_g scaffold00031.pathl .gene783 CL02839 is encoded by the nucleotides of SEQ ID No: 935 which encodes the amino acid sequence of SEQ ID No: 936. This enzyme is believed to have monooxygenase activity.
  • scaffold00031.pathl .gene823 is encoded by the nucleotides of SEQ ID No: 937 which encodes the amino acid sequence of SEQ ID No: 938. This enzyme is believed to have flavin-containing monooxygenase activity.
  • scaffold00050.pathl .genel 16 is encoded by the nucleotides of SEQ ID No: 939 which encodes the amino acid sequence of SEQ ID No: 940. This enzyme is believed to have flavin-containing monooxygenase activity.
  • scaffold00050.pathl .genel29 is encoded by the nucleotides of SEQ ID No: 941 which encodes the amino acid sequence of SEQ ID No: 942. This enzyme is believed to have monooxygenase activity.
  • scaffold00050.pathl .genel30 is encoded by the nucleotides of SEQ ID No: 943 which encodes the amino acid sequence of SEQ ID No: 944. This enzyme is believed to have monooxygenase activity.
  • scaffold00050.pathl .genel45 is encoded by the nucleotides of SEQ ID No: 947 which encodes the amino acid sequence of SEQ ID No: 948. This enzyme is believed to have linoleate diol synthase activity, monooxygenase activity, peroxidase activity.
  • scaffold00050.pathl .genel87 is encoded by the nucleotides of SEQ ID No: 949 which encodes the amino acid sequence of SEQ ID No: 950. This enzyme is believed to have monooxygenase activity.
  • scaffold00050.pathl .gene246 is encoded by the nucleotides of SEQ ID No: 953 which encodes the amino acid sequence of SEQ ID No: 954. This enzyme is believed to have flavin-containing monooxygenase activity.
  • Sequence Oxid-478 2418_g is encoded by the nucleotides of SEQ ID No: 955 which encodes the amino acid sequence of SEQ ID No: 956. This enzyme is believed to have cyclohexanone monooxygenase activity.
  • scaffold00050.pathl .gene325 is encoded by the nucleotides of SEQ ID No: 957 which encodes the amino acid sequence of SEQ ID No: 958. This enzyme is believed to have kynurenine 3 -monooxygenase activity.
  • scaffold00050.G900 scaffold00050.pathl .gene425 is encoded by the nucleotides of SEQ ID No: 959 which encodes the amino acid sequence of SEQ ID No: 960. This enzyme is believed to have flavin-containing monooxygenase activity.
  • sequence Oxid-481 2725_g scaffold00050.G1038 scaffold00050.pathl .gene511 is encoded by the nucleotides of SEQ ID No: 961 which encodes the amino acid sequence of SEQ ID No: 962. This enzyme is believed to have monooxygenase activity.
  • scaffold00050.pathl .gene514 is encoded by the nucleotides of SEQ ID No: 963 which encodes the amino acid sequence of SEQ ID No: 964. This enzyme is believed to have monooxygenase activity.
  • scaffold00050.pathl .gene515 is encoded by the nucleotides of SEQ ID No: 965 which encodes the amino acid sequence of SEQ ID No: 966. This enzyme is believed to have monooxygenase activity.
  • scaffold00050.pathl .gene702 is encoded by the nucleotides of SEQ ID No: 967 which encodes the amino acid sequence of SEQ ID No: 968. This enzyme is believed to have monooxygenase activity.
  • scaffold00050.pathl .gene747 is encoded by the nucleotides of SEQ ID No: 969 which encodes the amino acid sequence of SEQ ID No: 970. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-486 3022_g scaffold00050.G1491, scaffold00050.G1492 scaffold00050.pathl .gene784 is encoded by the nucleotides of SEQ ID No: 971 which encodes the amino acid sequence of SEQ ID No: 972.
  • This enzyme is believed to have monooxygenase activity.
  • scaffold00050.pathl .gene821 is encoded by the nucleotides of SEQ ID No: 973 which encodes the amino acid sequence of SEQ ID No: 974. This enzyme is believed to have monooxygenase activity.
  • scaffold00050.pathl .gene838 is encoded by the nucleotides of SEQ ID No: 975 which encodes the amino acid sequence of SEQ ID No: 976. This enzyme is believed to have monooxygenase activity.
  • scaffold00050.pathl .gene861 is encoded by the nucleotides of SEQ ID No: 977 which encodes the amino acid sequence of SEQ ID No: 978. This enzyme is believed to have aromatase activity, NADPH-hemoprotein reductase activity.
  • Sequence Oxid-490 3123_g scaffold00050.G1667 is encoded by the nucleotides of SEQ ID No: 979 which encodes the amino acid sequence of SEQ ID No: 980. This enzyme is believed to have monooxygenase activity.
  • scaffold00050.pathl .gene864 is encoded by the nucleotides of SEQ ID No: 981 which encodes the amino acid sequence of SEQ ID No: 982. This enzyme is believed to have monooxygenase activity.
  • scaffold00053.pathl .genel3 is encoded by the nucleotides of SEQ ID No: 983 which encodes the amino acid sequence of SEQ ID No: 984. This enzyme is believed to have monooxygenase activity.
  • scaffold00053.pathl .gene26 is encoded by the nucleotides of SEQ ID No: 985 which encodes the amino acid sequence of SEQ ID No: 986. This enzyme is believed to have monooxygenase activity.
  • scaffold00068.pathl .gene2 is encoded by the nucleotides of SEQ ID No: 987 which encodes the amino acid sequence of SEQ ID No: 988. This enzyme is believed to have monooxygenase activity.
  • scaffold00071.pathl .gene285 is encoded by the nucleotides of SEQ ID No: 989 which encodes the amino acid sequence of SEQ ID No: 990. This enzyme is believed to have sterol 14-demethylase activity.
  • scaffold00071.pathl .gene321 is encoded by the nucleotides of SEQ ID No: 991 which encodes the amino acid sequence of SEQ ID No: 992. This enzyme is believed to have oxidoreductase activity, acting on paired d activity.
  • scaffold00071.pathl .gene427 is encoded by the nucleotides of SEQ ID No: 993 which encodes the amino acid sequence of SEQ ID No: 994. This enzyme is believed to have phenol 2-monooxygenase activity.
  • scaffold00071.pathl .gene427 is encoded by the nucleotides of SEQ ID No: 995 which encodes the amino acid sequence of SEQ ID No: 996. This enzyme is believed to have monooxygenase activity.
  • scaffold00071.pathl .gene459 is encoded by the nucleotides of SEQ ID No: 997 which encodes the amino acid sequence of SEQ ID No: 998. This enzyme is believed to have monophenol monooxygenase activity.
  • Sequence Oxid-500 3805_g scaffold00071.pathl .gene492 is encoded by the nucleotides of SEQ ID No: 999 which encodes the amino acid sequence of SEQ ID No: 1000. This enzyme is believed to have dopamine beta- monooxygenase activity.
  • Sequence Oxid-501 3903_g scaffold00071.pathl .gene580 is encoded by the nucleotides of SEQ ID No: 1001 which encodes the amino acid sequence of SEQ ID No: 1002. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-502 3965_g scaffold00071.pathl .gene621 is encoded by the nucleotides of SEQ ID No: 1003 which encodes the amino acid sequence of SEQ ID No: 1004. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-503 4080_g scaffold00071.pathl .gene716 is encoded by the nucleotides of SEQ ID No: 1005 which encodes the amino acid sequence of SEQ ID No: 1006. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-504 4268_g scaffold00071.pathl .gene875 is encoded by the nucleotides of SEQ ID No: 1007 which encodes the amino acid sequence of SEQ ID No: 1008. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-505 428 l_g scaffold00071.pathl .gene885 is encoded by the nucleotides of SEQ ID No: 1009 which encodes the amino acid sequence of SEQ ID No: 1010. This enzyme is believed to have monooxygenase activity.
  • scaffold00071.pathl .gene 1013 is encoded by the nucleotides of SEQ ID No : 1011 which encodes the amino acid sequence of SEQ ID No: 1012. This enzyme is believed to have monooxygenase activity.
  • scaffold00071. athl . genel020 is encoded by the nucleotides of SEQ ID No: 1013 which encodes the amino acid sequence of SEQ ID No: 1014. This enzyme is believed to have monooxygenase activity.
  • pathl . genel 188 is encoded by the nucleotides of SEQ ID No: 1015 which encodes the amino acid sequence of SEQ ID No: 1016. This enzyme is believed to have monooxygenase activity.
  • pathl . genel246 is encoded by the nucleotides of SEQ ID No: 1017 which encodes the amino acid sequence of SEQ ID No: 1018. This enzyme is believed to have monooxygenase activity.
  • scaffold00071.pathl .genel812 is encoded by the nucleotides of SEQ ID No: 1019 which encodes the amino acid sequence of SEQ ID No: 1020. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-512 581 l_g scaffold00075. pathl . gene356 is encoded by the nucleotides of SEQ ID No: 1023 which encodes the amino acid sequence of SEQ ID No: 1024. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-513 5910_g is encoded by the nucleotides of SEQ ID No: 1025 which encodes the amino acid sequence of SEQ ID No: 1026. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-514 591 l_g is encoded by the nucleotides of SEQ ID No: 1027 which encodes the amino acid sequence of SEQ ID No: 1028. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-515 5928_g scaflbld00075.pathl .gene459 is encoded by the nucleotides of SEQ ID No: 1029 which encodes the amino acid sequence of SEQ ID No: 1030. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-516 5977_g scaffold00075.pathl .gene504 is encoded by the nucleotides of SEQ ID No: 1031 which encodes the amino acid sequence of SEQ ID No: 1032. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-517 6245_g scaffold00092.pathl .gene2 is encoded by the nucleotides of SEQ ID No: 1033 which encodes the amino acid sequence of SEQ ID No: 1034. This enzyme is believed to have monooxygenase activity.
  • the enzyme identified as Sequence Oxid-518 6253_g scaffold00092.pathl .gene9 is encoded by the nucleotides of SEQ ID No: 1035 which encodes the amino acid sequence of SEQ ID No: 1036. This enzyme is believed to have Oxidoreductase activity.
  • sequence Oxid-519 6265_g scaffold00092.pathl .gene20 is encoded by the nucleotides of SEQ ID No: 1037 which encodes the amino acid sequence of SEQ ID No: 1038. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-520 6288_g is encoded by the nucleotides of SEQ ID No: 1039 which encodes the amino acid sequence of SEQ ID No: 1040. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-521 6289_g scaffold00092.pathl .gene41 is encoded by the nucleotides of SEQ ID No: 1041 which encodes the amino acid sequence of SEQ ID No: 1042. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-522 629 l_g scaffold00092.pathl .gene42 is encoded by the nucleotides of SEQ ID No: 1043 which encodes the amino acid sequence of SEQ ID No: 1044. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-523 6302_g scaffold00092.G89 scaffold00092.pathl .gene52 is encoded by the nucleotides of SEQ ID No: 1045 which encodes the amino acid sequence of SEQ ID No: 1046. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-524 6303_g scaffold00092.pathl .gene52 is encoded by the nucleotides of SEQ ID No: 1047 which encodes the amino acid sequence of SEQ ID No: 1048. This enzyme is believed to have monooxygenase activity.
  • scaffold00092.pathl .gene72 is encoded by the nucleotides of SEQ ID No: 1049 which encodes the amino acid sequence of SEQ ID No: 1050. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-526 634 l_g is encoded by the nucleotides of SEQ ID No: 1051 which encodes the amino acid sequence of SEQ ID No: 1052. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-527 6343_g scaffold00092.pathl .gene76 is encoded by the nucleotides of SEQ ID No: 1053 which encodes the amino acid sequence of SEQ ID No: 1054. This enzyme is believed to have monooxygenase activity.
  • the enzyme identified as Sequence Oxid-528 6368_g scaffold00092.pathl .genel03 is encoded by the nucleotides of SEQ ID No: 1055 which encodes the amino acid sequence of SEQ ID No: 1056. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-529 6423_g scaffold00092.pathl .genel47 is encoded by the nucleotides of SEQ ID No: 1057 which encodes the amino acid sequence of SEQ ID No: 1058. This enzyme is believed to have monooxygenase activity.
  • scaffold00092.pathl .genel67 is encoded by the nucleotides of SEQ ID No: 1059 which encodes the amino acid sequence of SEQ ID No: 1060. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-531 6808_g is encoded by the nucleotides of SEQ ID No: 1061 which encodes the amino acid sequence of SEQ ID No: 1062. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-532 6820_g scaflbldOOl 15.pathl .gene2 is encoded by the nucleotides of SEQ ID No: 1063 which encodes the amino acid sequence of SEQ ID No: 1064. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-533 6828_g scaffold00120.pathl .genel is encoded by the nucleotides of SEQ ID No: 1065 which encodes the amino acid sequence of SEQ ID No: 1066. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-534 6864_g scaffold00122.pathl .gene29 is encoded by the nucleotides of SEQ ID No: 1067 which encodes the amino acid sequence of SEQ ID No: 1068. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-535 687 l_g is encoded by the nucleotides of SEQ ID No: 1069 which encodes the amino acid sequence of SEQ ID No: 1070. This enzyme is believed to have monooxygenase activity.
  • scaffold00122.pathl .genel08 is encoded by the nucleotides of SEQ ID No: 1071 which encodes the amino acid sequence of SEQ ID No: 1072. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-537 6952_g scaffold00122.pathl .genel09 is encoded by the nucleotides of SEQ ID No: 1073 which encodes the amino acid sequence of SEQ ID No: 1074. This enzyme is believed to have monooxygenase activity.
  • scaffold00122.pathl .genel 11 is encoded by the nucleotides of SEQ ID No: 1075 which encodes the amino acid sequence of SEQ ID No: 1076. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-539 7015_g scaffold00131.pathl .gene39 is encoded by the nucleotides of SEQ ID No: 1077 which encodes the amino acid sequence of SEQ ID No: 1078. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-541 7247_g scaffold00131.pathl .gene258 is encoded by the nucleotides of SEQ ID No: 1081 which encodes the amino acid sequence of SEQ ID No: 1082. This enzyme is believed to have monooxygenase activity.
  • scaffold00131.pathl .gene445 is encoded by the nucleotides of SEQ ID No: 1083 which encodes the amino acid sequence of SEQ ID No: 1084. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-543 7522_g scaffold00131.pathl .gene518 is encoded by the nucleotides of SEQ ID No: 1085 which encodes the amino acid sequence of SEQ ID No: 1086. This enzyme is believed to have monooxygenase activity.
  • Sequence Oxid-544 7568_g scaffold00131.pathl .gene546 is encoded by the nucleotides of SEQ ID No: 1087 which encodes the amino acid sequence of SEQ ID No: 1088. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-545 769 l_g scaffold00131.pathl .gene649 is encoded by the nucleotides of SEQ ID No: 1089 which encodes the amino acid sequence of SEQ ID No: 1090. This enzyme is believed to have monooxygenase activity.
  • scaffold00131.pathl .genel016 is encoded by the nucleotides of SEQ ID No: 1091 which encodes the amino acid sequence of SEQ ID No: 1092. This enzyme is believed to have monooxygenase activity.
  • scaffold00131.pathl .gene218 is encoded by the nucleotides of SEQ ID No: 1093 which encodes the amino acid sequence of SEQ ID No: 1094. This enzyme is believed to have monooxygenase activity.
  • sequence Oxid-548 7350_g scaffold00131.pathl .gene355 is encoded by the nucleotides of SEQ ID No: 1095 which encodes the amino acid sequence of SEQ ID No: 1096. This enzyme is believed to have trimethyllysine dioxygenase activity.
  • the enzyme identified as Sequence Oxid-549 735 l_g scaflbld00131.pathl .gene356 is encoded by the nucleotides of SEQ ID No: 1097 which encodes the amino acid sequence of SEQ ID No: 1098. This enzyme is believed to have dioxygenase activity.
  • scaffold00131.pathl .gene479 is encoded by the nucleotides of SEQ ID No: 1099 which encodes the amino acid sequence of SEQ ID NO: 1100. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-551 8028_g is encoded by the nucleotides of SEQ ID No: 1101 which encodes the amino acid sequence of SEQ ID No: 1102. This enzyme is believed to have dioxygenase activity.
  • scaffold00131.pathl .genel l38 is encoded by the nucleotides of SEQ ID No: 1103 which encodes the amino acid sequence of SEQ ID No: 1104. This enzyme is believed to have oxidoreductase activity.
  • sequence Oxid-553 8686_g scaffold00142.pathl .genel49 is encoded by the nucleotides of SEQ ID No: 1105 which encodes the amino acid sequence of SEQ ID No: 1106. This enzyme is believed to have oxidoreductase activity.
  • scaffold00142.pathl .genel94 is encoded by the nucleotides of SEQ ID No: 1107 which encodes the amino acid sequence of SEQ ID No: 1108. This enzyme is believed to have dioxygenase activity.
  • Sequence Oxid-555 8896_g scaffold00169.pathl .gene4 is encoded by the nucleotides of SEQ ID No: 1109 which encodes the amino acid sequence of SEQ ID No: 1110. This enzyme is believed to have oxidoreductase activity.
  • scaffold00227.pathl .gene32 is encoded by the nucleotides of SEQ ID No: 1111 which encodes the amino acid sequence of SEQ ID No: 1112. This enzyme is believed to have dioxygenase activity.
  • the enzyme identified as Sequence Oxid-557 9504_g scaffold00227.G198 scaffold00227.pathl .genel02 is encoded by the nucleotides of SEQ ID No: 1113 which encodes the amino acid sequence of SEQ ID No: 1114. This enzyme is believed to have dioxygenase activity.
  • Sequence Oxid-558 566_g scaffold00016.pathl .gene487 is encoded by the nucleotides of SEQ ID No: 1115 which encodes the amino acid sequence of SEQ ID No: 1116. This enzyme is believed to have dioxygenase activity.
  • scaffold00031.pathl .gene76 is encoded by the nucleotides of SEQ ID No: 1117 which encodes the amino acid sequence of SEQ ID No: 1118. This enzyme is believed to have dioxygenase activity.
  • pathl . genel 17 is encoded by the nucleotides of SEQ ID No: 1119 which encodes the amino acid sequence of SEQ ID No: 1120. This enzyme is believed to have dioxygenase activity.
  • sequence Oxid-561 1426_g scaffold00031. pathl . gene433 is encoded by the nucleotides of SEQ ID No: 1121 which encodes the amino acid sequence of SEQ ID No: 1122. This enzyme is believed to have oxidoreductase activity.
  • pathl . gene891 is encoded by the nucleotides of SEQ ID No: 1123 which encodes the amino acid sequence of SEQ ID No: 1124. This enzyme is believed to have dioxygenase activity.
  • sequence Oxid-563 2265_g scaffold00050.pathl . genel 14 is encoded by the nucleotides of SEQ ID No: 1125 which encodes the amino acid sequence of SEQ ID No: 1126. This enzyme is believed to have dioxygenase activity.
  • scaffold00071.G251 scaffold00071.pathl .genel 14 is encoded by the nucleotides of SEQ ID No: 1127 which encodes the amino acid sequence of SEQ ID No: 1128. This enzyme is believed to have dioxygenase activity.
  • sequence Oxid-565 3432_g scaffold00071. pathl . genel66 is encoded by the nucleotides of SEQ ID No: 1129 which encodes the amino acid sequence of SEQ ID No: 1130. This enzyme is believed to have dioxygenase activity.
  • scaffold00071.pathl .genel225 is encoded by the nucleotides of SEQ ID No: 1131 which encodes the amino acid sequence of SEQ ID No: 1132. This enzyme is believed to have oxidoreductase activity.
  • scaffold00071. athl . genel717 is encoded by the nucleotides of SEQ ID No: 1133 which encodes the amino acid sequence of SEQ ID No: 1134. This enzyme is believed to have dioxygenase activity.
  • Sequence Oxid-568 5645_g is encoded by the nucleotides of SEQ ID No: 1135 which encodes the amino acid sequence of SEQ ID No: 1136.
  • This enzyme is believed to have dioxygenase activity.
  • sequence Oxid-569 6086_g scaffold00075. athl . gene608 is encoded by the nucleotides of SEQ ID No: 1137 which encodes the amino acid sequence of SEQ ID No: 1138. This enzyme is believed to have sulfonate dioxygenase activity.
  • scaffold00016.pathl .gene5 is encoded by the nucleotides of SEQ ID No: 1139 which encodes the amino acid sequence of SEQ ID No: 1140. This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-571 28_g is encoded by the nucleotides of
  • SEQ ID No: 1141 which encodes the amino acid sequence of SEQ ID No: 1142.
  • This enzyme is believed to have oxidoreductase activity.
  • SEQ ID No: 1143 which encodes the amino acid sequence of SEQ ID No: 1144.
  • This enzyme is believed to have oxidoreductase activity.
  • SEQ ID No: 1145 which encodes the amino acid sequence of SEQ ID No: 1146.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-574 105_g is encoded by the nucleotides of SEQ ID No: 1147 which encodes the amino acid sequence of SEQ ID No: 1148.
  • Sequence Oxid-575 174_g is encoded by the nucleotides of SEQ ID No: 1149 which encodes the amino acid sequence of SEQ ID No: 1150.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-576 182_g is encoded by the nucleotides of SEQ ID No: 1151 which encodes the amino acid sequence of SEQ ID No: 1152.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-577 290_g is encoded by the nucleotides of SEQ ID No: 1153 which encodes the amino acid sequence of SEQ ID No: 1154.
  • This enzyme is believed to have glutamate synthase (NADPH) activity.
  • Sequence Oxid-578 308_g is encoded by the nucleotides of SEQ ID No: 1155 which encodes the amino acid sequence of SEQ ID No: 1156.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-579 309_g is encoded by the nucleotides of SEQ ID No: 1157 which encodes the amino acid sequence of SEQ ID No: 1158.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-580 367_g is encoded by the nucleotides of SEQ ID No: 1159 which encodes the amino acid sequence of SEQ ID No: 1160.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-581 394_g is encoded by the nucleotides of SEQ ID No: 1161 which encodes the amino acid sequence of SEQ ID No: 1162.
  • This enzyme is believed to have Oxidase activity.
  • Sequence Oxid-582 429_g is encoded by the nucleotides of SEQ ID No: 1163 which encodes the amino acid sequence of SEQ ID No: 1164.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-583 455_g is encoded by the nucleotides of SEQ ID No: 1165 which encodes the amino acid sequence of SEQ ID No: 1166.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-584 473_g is encoded by the nucleotides of SEQ ID No: 1167 which encodes the amino acid sequence of SEQ ID No: 1168.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-585 541_g is encoded by the nucleotides of SEQ ID No: 1169 which encodes the amino acid sequence of SEQ ID No: 1170.
  • Sequence Oxid-586 645_g is encoded by the nucleotides of SEQ ID No: 1171 which encodes the amino acid sequence of SEQ ID No: 1172.
  • This enzyme is believed to have oxidase activity.
  • Sequence Oxid-587 697_g is encoded by the nucleotides of SEQ ID No: 1173 which encodes the amino acid sequence of SEQ ID No: 1174.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-588 714_g is encoded by the nucleotides of SEQ ID No: 1175 which encodes the amino acid sequence of SEQ ID No: 1176.
  • This enzyme is believed to have carbonyl reductase (NADPH) activity.
  • Sequence Oxid-589 719_g is encoded by the nucleotides of SEQ ID No: 1177 which encodes the amino acid sequence of SEQ ID No: 1178.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-590 730_g is encoded by the nucleotides of SEQ ID No: 1179 which encodes the amino acid sequence of SEQ ID No: 1180.
  • This enzyme is believed to have glutathione-disulfide reductase activity.
  • Sequence Oxid-591 737_g is encoded by the nucleotides of SEQ ID No: 1181 which encodes the amino acid sequence of SEQ ID No: 1182.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-592 829_g is encoded by the nucleotides of SEQ ID No: 1183 which encodes the amino acid sequence of SEQ ID No: 1184.
  • This enzyme is believed to have hydroxylase activity.
  • Sequence Oxid-593 835_g is encoded by the nucleotides of SEQ ID No: 1185 which encodes the amino acid sequence of SEQ ID No: 1186.
  • This enzyme is believed to have ribonucleotide reductase activity.
  • Sequence Oxid-594 940_g is encoded by the nucleotides of SEQ ID No: 1187 which encodes the amino acid sequence of SEQ ID No: 1188.
  • This enzyme is believed to have catalase activity.
  • Sequence Oxid-595 961_g is encoded by the nucleotides of SEQ ID No: 1189 which encodes the amino acid sequence of SEQ ID No: 1190.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-596 987_g is encoded by the nucleotides of SEQ ID No: 1191 which encodes the amino acid sequence of SEQ ID No: 1192.
  • Sequence Oxid-597 999_g is encoded by the nucleotides of SEQ ID No: 1193 which encodes the amino acid sequence of SEQ ID No: 1194.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-598 1025_g is encoded by the nucleotides of SEQ ID No: 1195 which encodes the amino acid sequence of SEQ ID No: 1196.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-599 1040_g is encoded by the nucleotides of SEQ ID No: 1197 which encodes the amino acid sequence of SEQ ID No: 1198.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-600 1087_g is encoded by the nucleotides of SEQ ID No: 1199 which encodes the amino acid sequence of SEQ ID No: 1200.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-601 1115_g is encoded by the nucleotides of SEQ ID No: 1201 which encodes the amino acid sequence of SEQ ID No: 1202.
  • This enzyme is believed to have urate oxidase activity.
  • Sequence Oxid-602 1147_g is encoded by the nucleotides of SEQ ID No: 1203 which encodes the amino acid sequence of SEQ ID No: 1204.
  • This enzyme is believed to have ribonucleoside-diphosphate reductase activity.
  • Sequence Oxid-603 1220_g is encoded by the nucleotides of SEQ ID No: 1205 which encodes the amino acid sequence of SEQ ID No: 1206.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-604 1222_g is encoded by the nucleotides of SEQ ID No: 1207 which encodes the amino acid sequence of SEQ ID No: 1208.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-605 1223_g is encoded by the nucleotides of SEQ ID No: 1209 which encodes the amino acid sequence of SEQ ID No: 1210.
  • This enzyme is believed to have pyrroline-5-carboxylate reductase activity.
  • Sequence Oxid-606 1225_g is encoded by the nucleotides of SEQ ID No: 1211 which encodes the amino acid sequence of SEQ ID No: 1212.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-607 1226_g is encoded by the nucleotides of SEQ ID No: 1213 which encodes the amino acid sequence of SEQ ID No: 1214.
  • Sequence Oxid-608 1232_g is encoded by the nucleotides of SEQ ID No: 1215 which encodes the amino acid sequence of SEQ ID No: 1216.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-609 1250_g is encoded by the nucleotides of SEQ ID No: 1217 which encodes the amino acid sequence of SEQ ID No: 1218.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-610 1272_g is encoded by the nucleotides of SEQ ID No: 1219 which encodes the amino acid sequence of SEQ ID No: 1220.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-611 1273_g is encoded by the nucleotides of SEQ ID No: 1221 which encodes the amino acid sequence of SEQ ID No: 1222.
  • This enzyme is believed to have oxidoreductase activity.
  • Sequence Oxid-612 1298_g is encoded by the nucleotides of SEQ ID No: 1223 which encodes the amino acid sequence of SEQ ID No: 1224.
  • This enzyme is believed to have pyrroline-5-carboxylate reductase activity.
  • Sequence Oxid-613 1306_g is encoded by the nucleotides of SEQ ID No: 1225 which encodes the amino acid sequence of SEQ ID No: 1226.
  • This enzyme is believed to have oxidoreductase activity.

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Abstract

Cette invention concerne de nouvelles enzymes et de nouveaux procédés de production de ces enzymes. L'invention concerne plus précisément des enzymes d'origine fongique entrant dans la catégorie des oxydoréductases et produites par des champignons. L'invention décrit les molécules d'acide nucléique codant ces enzymes, des compositions, des cellules hôtes recombinées et modifiées génétiquement, et leurs procédés d'utilisation. L'invention concerne aussi des procédés d'utilisation de ces nouvelles enzymes et des compositions comportant ces enzymes dans différents autres procédés, notamment la désinfection de produits alimentaires, le désencrage et la décoloration biologique de la farine, du coton, du papier et de la pulpe, et le traitement anti-pollution. L'invention concerne par ailleurs un procédé d'aide à la conversion de la biomasse lignocellulosique en sucres fermentescibles avec des enzymes dégradant la matière lignocellulosique, et de nouvelles combinaisons d'enzymes, notamment celles qui assurent une libération synergique de sucres issus de la biomasse végétale.
PCT/US2011/060970 2010-11-16 2011-11-16 Nouvelles oxydoréductases fongiques Ceased WO2012068236A2 (fr)

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WO2014056921A3 (fr) * 2012-10-12 2014-06-05 Novozymes A/S Polypeptides à activité peroxygénase
WO2014056919A3 (fr) * 2012-10-12 2014-06-12 Novozymes A/S Polypeptides à activité peroxygénase
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WO2015081139A1 (fr) 2013-11-26 2015-06-04 Novozymes A/S Compositions enzymatiques et leurs utilisations
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CN104694510A (zh) * 2015-02-10 2015-06-10 江南大学 嗜热侧孢霉生产耐热超氧化物歧化酶(sod)的方法
WO2015187935A1 (fr) 2014-06-06 2015-12-10 Novozymes A/S Compositions enzymatiques et leurs utilisations
WO2016145358A1 (fr) 2015-03-12 2016-09-15 Novozymes A/S Hydrolyse enzymatique avec des enzymes hémicellulolytiques
WO2016145350A1 (fr) 2015-03-12 2016-09-15 Novozymes A/S Hydrolyse enzymatique à étages multiple d'une biomasse lignocellulosique
WO2016142536A1 (fr) * 2015-03-11 2016-09-15 Genencor International B.V. Activité enzymatique de polysaccharide monooxygénase lytique
WO2016145363A1 (fr) 2015-03-12 2016-09-15 Novozymes A/S Hydrolyse enzymatique multi-étape d'une biomasse lignocellulosique faisant appel à une oxydoréductase et à un polypeptide aa9
WO2016207351A3 (fr) * 2015-06-24 2017-03-02 Genencor International B.V. Polypeptides ayant une activité de déméthylation
WO2017040907A1 (fr) 2015-09-04 2017-03-09 Novozymes A/S Procédés d'inhibition de l'inactivation, catalysée par la monooxygénase polysaccharidique lytique aa9, de compositions enzymatiques
US9663806B2 (en) * 2012-03-31 2017-05-30 Novozymes A/S Expoxidation using peroxygenases
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US9611459B2 (en) 2012-10-12 2017-04-04 Novozymes A/S Polypeptides having peroxygenase activity
WO2014056927A3 (fr) * 2012-10-12 2014-06-12 Novozymes A/S Polypeptides à activité peroxygénase
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US20180051305A1 (en) * 2015-03-11 2018-02-22 Genencor International B.V. Enzymatic activity of lytic polysaccharide monooxygenase
WO2016142536A1 (fr) * 2015-03-11 2016-09-15 Genencor International B.V. Activité enzymatique de polysaccharide monooxygénase lytique
WO2016145363A1 (fr) 2015-03-12 2016-09-15 Novozymes A/S Hydrolyse enzymatique multi-étape d'une biomasse lignocellulosique faisant appel à une oxydoréductase et à un polypeptide aa9
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WO2016207351A3 (fr) * 2015-06-24 2017-03-02 Genencor International B.V. Polypeptides ayant une activité de déméthylation
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WO2018201050A1 (fr) * 2017-04-28 2018-11-01 Board Of Trustees Of Michigan State University Biosynthèse de tryptathionine par la flavine mono-oxygénase 1 (fmo1)
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