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WO2012050353A3 - Method whereby the base sequence of a target polynucleotide is non-specifically extended - Google Patents

Method whereby the base sequence of a target polynucleotide is non-specifically extended Download PDF

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Publication number
WO2012050353A3
WO2012050353A3 PCT/KR2011/007561 KR2011007561W WO2012050353A3 WO 2012050353 A3 WO2012050353 A3 WO 2012050353A3 KR 2011007561 W KR2011007561 W KR 2011007561W WO 2012050353 A3 WO2012050353 A3 WO 2012050353A3
Authority
WO
WIPO (PCT)
Prior art keywords
target polynucleotide
pcr
present
base sequence
specifically extended
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2011/007561
Other languages
French (fr)
Korean (ko)
Other versions
WO2012050353A2 (en
Inventor
김성진
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2012050353A2 publication Critical patent/WO2012050353A2/en
Publication of WO2012050353A3 publication Critical patent/WO2012050353A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)

Abstract

The present invention relates to a method whereby the base-sequence of a target polynucleotide is non-specifically extended to the base sequence length required for an intended purpose. The present invention can improve the sensitivity of detection or the reproducibility of detection of target polynucleotides, and can be put to use as a basic technique that can be put to service in various technologies for analysing single nucleotide polymorphism (SNP) and whether a target polynucleotide is present in a sample by using electrical-signal based biosensors and microfluidic chips and PCR amplification products; for example various technologies such as PCR-SSCP, microarray chips, multiplex PCR, real-time PCR, RFLP and electrophoretic analysis.
PCT/KR2011/007561 2010-10-15 2011-10-12 Method whereby the base sequence of a target polynucleotide is non-specifically extended Ceased WO2012050353A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0101064 2010-10-15
KR20100101064 2010-10-15

Publications (2)

Publication Number Publication Date
WO2012050353A2 WO2012050353A2 (en) 2012-04-19
WO2012050353A3 true WO2012050353A3 (en) 2012-08-30

Family

ID=45938795

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/007561 Ceased WO2012050353A2 (en) 2010-10-15 2011-10-12 Method whereby the base sequence of a target polynucleotide is non-specifically extended

Country Status (2)

Country Link
KR (1) KR101726238B1 (en)
WO (1) WO2012050353A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101690279B1 (en) * 2015-02-09 2016-12-28 (주) 하임바이오텍 A kit and method for detecting single nucleotide polymorphic DNA
EP3348650B1 (en) * 2016-08-05 2020-12-23 Heimbiotek Inc. Kit and method for detecting single nucleotide polymorphism
CN109957611B (en) * 2019-03-22 2020-06-26 健生生物技术有限公司 Specific quantitative PCR reaction mixed solution, miRNA quantitative detection kit and detection method
CN110157795A (en) * 2019-05-30 2019-08-23 北京和合医学诊断技术股份有限公司 A kind of detection method of personalized medicine gene pleiomorphism

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040042021A (en) * 2002-11-12 2004-05-20 삼성전자주식회사 A method for detecting a PCR product by measuring a electrical signal
KR100590546B1 (en) * 2004-02-28 2006-06-19 삼성전자주식회사 Micro PCR device, nucleic acid amplification method using the same and method for measuring the concentration of PCR amplification products
KR20090103336A (en) * 2008-03-28 2009-10-01 디지탈 지노믹스(주) High sensitive biosensor, biochip comprising the same and manufacturing method therefor
KR100969667B1 (en) * 2008-03-24 2010-07-14 디지탈 지노믹스(주) Method for detecting bioactive substance electrically and biochip for same

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2003040367A1 (en) * 2001-11-08 2005-03-03 三光純薬株式会社 Method for forming self-assembly by oligonucleotide synthesized by gene amplification reaction, method for detecting self-assembly and gene
KR20070031957A (en) * 2004-05-28 2007-03-20 나노겐 인코포레이티드 Nano-sized electron detection system and its manufacturing method
DE602007012045D1 (en) * 2006-06-06 2011-03-03 Gen Probe Inc Labeled Oligonucleotides and Their Use in Nucleic Acid Reinforcement Methods
WO2009102878A2 (en) * 2008-02-12 2009-08-20 Nugen Technologies, Inc. Method for archiving and clonal expansion

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040042021A (en) * 2002-11-12 2004-05-20 삼성전자주식회사 A method for detecting a PCR product by measuring a electrical signal
KR100590546B1 (en) * 2004-02-28 2006-06-19 삼성전자주식회사 Micro PCR device, nucleic acid amplification method using the same and method for measuring the concentration of PCR amplification products
KR100969667B1 (en) * 2008-03-24 2010-07-14 디지탈 지노믹스(주) Method for detecting bioactive substance electrically and biochip for same
KR20090103336A (en) * 2008-03-28 2009-10-01 디지탈 지노믹스(주) High sensitive biosensor, biochip comprising the same and manufacturing method therefor

Also Published As

Publication number Publication date
KR101726238B1 (en) 2017-04-12
KR20120047767A (en) 2012-05-14
WO2012050353A2 (en) 2012-04-19

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