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WO2012040579A3 - Molecular screening across defined stiffness matrices - Google Patents

Molecular screening across defined stiffness matrices Download PDF

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Publication number
WO2012040579A3
WO2012040579A3 PCT/US2011/052990 US2011052990W WO2012040579A3 WO 2012040579 A3 WO2012040579 A3 WO 2012040579A3 US 2011052990 W US2011052990 W US 2011052990W WO 2012040579 A3 WO2012040579 A3 WO 2012040579A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecular screening
defined stiffness
stiffness matrices
methods
matrices
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/052990
Other languages
French (fr)
Other versions
WO2012040579A2 (en
Inventor
Daniel Tschumperlin
Justin Mih
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of WO2012040579A2 publication Critical patent/WO2012040579A2/en
Publication of WO2012040579A3 publication Critical patent/WO2012040579A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4703—Regulators; Modulating activity

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This application related to methods for cell-based screening featuring substrates of user-defined stiffness. The methods may be used to assess the magnitude and character of cellular drug responses across a physiological stiffness range.
PCT/US2011/052990 2010-09-23 2011-09-23 Molecular screening across defined stiffness matrices Ceased WO2012040579A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38594210P 2010-09-23 2010-09-23
US61/385,942 2010-09-23

Publications (2)

Publication Number Publication Date
WO2012040579A2 WO2012040579A2 (en) 2012-03-29
WO2012040579A3 true WO2012040579A3 (en) 2012-06-28

Family

ID=45874402

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/052990 Ceased WO2012040579A2 (en) 2010-09-23 2011-09-23 Molecular screening across defined stiffness matrices

Country Status (1)

Country Link
WO (1) WO2012040579A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2438391B1 (en) * 2012-06-12 2014-10-22 Universidad De Zaragoza CELLULAR CULTURE DEVICE AND METHOD ASSOCIATED WITH SUCH DEVICE
EP3030646A4 (en) * 2013-08-07 2017-03-22 Agency For Science, Technology And Research Methods, apparatuses, and systems for cell and tissue culture

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
KOSTIC, A. ET AL.: "Differential matrix rigidity response in breast cancer cell lines corelates with the tissue tropism", PLOS ONE., vol. 4, no. 7, 23 July 2009 (2009-07-23), pages E6361 *
LAU, K. C. ET AL.: "Automated imaging of circulating fluorocytes for the diagnosis of erythropoietic protoporphyria: A pilot study for population screening", JOURNAL OF MEDICAL SCREENING., vol. 15, no. 4, 2008, pages 199 - 203 *
MCDANIEL, D. P. ET AL.: "The stiffness of collagen fibrils influences vascular smooth muscle cell phenotype", BIOPHYSICAL JOURNAL., vol. 92, no. 5, 8 December 2006 (2006-12-08), pages 1759 - 1769 *
REHFELDT, F. ET AL.: "Cell responses to the mechanochemical microenvironment - Implications for regenerative medicine and drug delivery", ADVANCED DRUG DELIVERY REVIEWS., vol. 59, 14 August 2007 (2007-08-14), pages 1329 - 1339, XP022343631, DOI: doi:10.1016/j.addr.2007.08.007 *
SEMLER, E. J.: "Engineering hepatocellular morphogenesis and function via ligand-presenting hydrogels with graded mechanical compliance", BIOTECHNOLOGY AND BIOENGINEERING., vol. 89, no. 3, 22 December 2004 (2004-12-22), pages 296 - 307 *

Also Published As

Publication number Publication date
WO2012040579A2 (en) 2012-03-29

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