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WO2011130584A3 - Procédés de mesure de l'activité enzymatique utiles pour la détermination de la viabilité cellulaire dans des échantillons non purifiés - Google Patents

Procédés de mesure de l'activité enzymatique utiles pour la détermination de la viabilité cellulaire dans des échantillons non purifiés Download PDF

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Publication number
WO2011130584A3
WO2011130584A3 PCT/US2011/032600 US2011032600W WO2011130584A3 WO 2011130584 A3 WO2011130584 A3 WO 2011130584A3 US 2011032600 W US2011032600 W US 2011032600W WO 2011130584 A3 WO2011130584 A3 WO 2011130584A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
enzyme activity
cell viability
purified samples
determining cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/032600
Other languages
English (en)
Other versions
WO2011130584A2 (fr
Inventor
Shawn Mark O'hara
Daniel R. Zweitzig
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zeus Scientific Inc
Original Assignee
Zeus Scientific Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44799329&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2011130584(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to EP19187658.0A priority Critical patent/EP3617320B1/fr
Priority to ES11769633T priority patent/ES2644258T5/es
Priority to EP11769633.6A priority patent/EP2558600B2/fr
Priority to KR1020127030167A priority patent/KR20130094714A/ko
Priority to EP17194583.5A priority patent/EP3284831B1/fr
Priority to AU2011239587A priority patent/AU2011239587B2/en
Priority to KR1020187021294A priority patent/KR102068136B1/ko
Priority to AP2012006530A priority patent/AP2012006530A0/xx
Priority to CN201180019406.9A priority patent/CN103328652B/zh
Priority to JP2013505162A priority patent/JP2013537399A/ja
Priority to EA201291001A priority patent/EA201291001A1/ru
Application filed by Zeus Scientific Inc filed Critical Zeus Scientific Inc
Priority to US13/641,480 priority patent/US20130196318A1/en
Priority to SG2012076097A priority patent/SG184563A1/en
Priority to CA2796676A priority patent/CA2796676C/fr
Priority to PH1/2012/502065A priority patent/PH12012502065A1/en
Publication of WO2011130584A2 publication Critical patent/WO2011130584A2/fr
Anticipated expiration legal-status Critical
Priority to IL222510A priority patent/IL222510B/en
Priority to ZA2012/08522A priority patent/ZA201208522B/en
Publication of WO2011130584A3 publication Critical patent/WO2011130584A3/fr
Priority to US14/737,736 priority patent/US10870894B2/en
Priority to US14/825,092 priority patent/US10876173B2/en
Priority to PH12016500300A priority patent/PH12016500300A1/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention a pour objet général le domaine de la détection de micro-organismes, en particulier de la détection de bactéries, des procédés de mesure de l'activité enzymatique, telle que l'activité de l'ADN polymérase, et concerne en particulier de tels procédés réalisés sur des lysats microbiens bruts, utiles pour la détermination des activités enzymatiques microbiennes qui peuvent être reliées à des générateurs de signaux d'amplification tels que des techniques d'amplification en chaîne par polymérase (ACP) en temps réel, ce qui permet la détermination des pathogènes microbiens dans des échantillons tels que du sang et d'autres fluides corporels non purifiés. Cette invention concerne aussi des réactifs destinés à être utilisés dans de tels procédés, et des kits d'essai comprenant de tels réactifs pour la mise en œuvre de ces procédés.
PCT/US2011/032600 2010-04-16 2011-04-15 Procédés de mesure de l'activité enzymatique utiles pour la détermination de la viabilité cellulaire dans des échantillons non purifiés Ceased WO2011130584A2 (fr)

Priority Applications (20)

Application Number Priority Date Filing Date Title
PH1/2012/502065A PH12012502065A1 (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
US13/641,480 US20130196318A1 (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
EP11769633.6A EP2558600B2 (fr) 2010-04-16 2011-04-15 Procédés de mesure de l'activité enzymatique utiles pour la détermination de la viabilité cellulaire dans des échantillons non purifiés
KR1020127030167A KR20130094714A (ko) 2010-04-16 2011-04-15 비-정제된 샘플에서 세포 생존력을 결정하는데에 유용한 효소 활성을 측정하기 위한 방법
EP17194583.5A EP3284831B1 (fr) 2010-04-16 2011-04-15 Procédés de mesure de l'activité enzymatique utiles pour la détermination de la viabilité cellulaire dans des échantillons non purifiés
AU2011239587A AU2011239587B2 (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
KR1020187021294A KR102068136B1 (ko) 2010-04-16 2011-04-15 비-정제된 샘플에서 세포 생존력을 결정하는데에 유용한 효소 활성을 측정하기 위한 방법
AP2012006530A AP2012006530A0 (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
CN201180019406.9A CN103328652B (zh) 2010-04-16 2011-04-15 测量用于测定未纯化的样品中细胞生活力的酶活性的方法
ES11769633T ES2644258T5 (es) 2010-04-16 2011-04-15 Métodos de medición de la actividad enzimática útiles para determinar la viabilidad celular en muestras no purificadas
EA201291001A EA201291001A1 (ru) 2010-04-16 2011-04-15 Способы измерения ферментативной активности, полезные при определении жизнеспособности клеток в неочищенных образцах
EP19187658.0A EP3617320B1 (fr) 2010-04-16 2011-04-15 Procédés permettant de mesurer l'activité enzymatique utile dans la détermination de la viabilité cellulaire dans des échantillons non purifiés
CA2796676A CA2796676C (fr) 2010-04-16 2011-04-15 Procedes de mesure de l'activite enzymatique utiles pour la determination de la viabilite cellulaire dans des echantillons non purifies
JP2013505162A JP2013537399A (ja) 2010-04-16 2011-04-15 非精製サンプル中の細胞生存能力を決定することにおいて有用な酵素活性を測定するための方法
SG2012076097A SG184563A1 (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
IL222510A IL222510B (en) 2010-04-16 2012-10-17 Methods for measuring enzymatic activity are useful for determining the presence of a microorganism
ZA2012/08522A ZA201208522B (en) 2010-04-16 2012-11-13 Methods for measuring enzyme activity useful in determining cell viability in non-purified sapmles
US14/737,736 US10870894B2 (en) 2010-04-16 2015-06-12 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
US14/825,092 US10876173B2 (en) 2010-04-16 2015-08-12 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
PH12016500300A PH12016500300A1 (en) 2010-04-16 2016-02-12 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US32494910P 2010-04-16 2010-04-16
US32493910P 2010-04-16 2010-04-16
US61/324,949 2010-04-16
US61/324,939 2010-04-16
US32541310P 2010-04-19 2010-04-19
US61/325,413 2010-04-19

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US13/641,480 A-371-Of-International US20130196318A1 (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
US14/737,736 Division US10870894B2 (en) 2010-04-16 2015-06-12 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
US14/825,092 Continuation US10876173B2 (en) 2010-04-16 2015-08-12 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Publications (2)

Publication Number Publication Date
WO2011130584A2 WO2011130584A2 (fr) 2011-10-20
WO2011130584A3 true WO2011130584A3 (fr) 2013-07-11

Family

ID=44799329

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/032600 Ceased WO2011130584A2 (fr) 2010-04-16 2011-04-15 Procédés de mesure de l'activité enzymatique utiles pour la détermination de la viabilité cellulaire dans des échantillons non purifiés

Country Status (16)

Country Link
US (3) US20130196318A1 (fr)
EP (3) EP2558600B2 (fr)
JP (2) JP2013537399A (fr)
KR (2) KR102068136B1 (fr)
CN (1) CN103328652B (fr)
AP (1) AP2012006530A0 (fr)
AU (2) AU2011239587B2 (fr)
CA (1) CA2796676C (fr)
EA (1) EA201291001A1 (fr)
ES (3) ES2644258T5 (fr)
MY (1) MY161567A (fr)
NZ (2) NZ701866A (fr)
PH (2) PH12012502065A1 (fr)
SG (2) SG184563A1 (fr)
WO (1) WO2011130584A2 (fr)
ZA (1) ZA201208522B (fr)

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NZ701866A (en) 2010-04-16 2016-07-29 Zeus Scientific Inc Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
IN2014DN06516A (fr) * 2012-01-05 2015-06-12 Zeus Scientific Inc
US20160083776A1 (en) * 2012-04-12 2016-03-24 Zeus Scientific, Inc. Methods for Measuring Polymerase Activity Useful for Sensitive, Quantitative Measurements of Any Polymerase Extension Activity and for Determining the Presence of Viable Cells
GB2528647A (en) * 2014-07-10 2016-02-03 Momentum Bioscience Ltd Detecting viable microorganisms
GB201412316D0 (en) 2014-07-10 2014-08-27 Momentum Bioscience Ltd Detecting the absence of micro-organisms
US9879328B2 (en) * 2014-11-21 2018-01-30 GeneWeave Biosciences, Inc. Mechanisms of antimicrobial susceptibility
US10662484B1 (en) 2016-03-24 2020-05-26 Bench in a Box, LLC Two universal duplex primer sets and a combined real-time PCR and high resolution melt analysis assay for the amplification of gram-positive and gram-negative bacteria
GB201805479D0 (en) 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
US20230112608A1 (en) 2021-10-13 2023-04-13 Disruptive Oil And Gas Technologies Corp Nanobubble dispersions generated in electrochemically activated solutions
CN114032285B (zh) * 2021-11-24 2022-05-24 北京擎科生物科技有限公司 PolyA聚合酶活性检测方法
WO2025122959A1 (fr) * 2023-12-07 2025-06-12 California Institute Of Technology Procédés pour l'alignement traduit des données transcriptomiques à résolution unicellulaire
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WO2009007719A2 (fr) * 2007-07-09 2009-01-15 Iseao Technologies Limited Détection améliorée de micro-organismes
EP1205559B1 (fr) * 1999-07-28 2009-06-24 Kaken Pharmaceutical Co., Ltd. Methode de detection des micro-organismes pathogenes et des agents antimicrobiens, methode d'evaluation de l'effet medicamenteux des agents antimicrobiens, et agents antimicrobiens
US20090275063A1 (en) * 2005-05-03 2009-11-05 Marc Green Distinguishing cells in a sample by inactivating extracellular enzyme before releasing intracellular enzyme

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US6054278A (en) * 1997-05-05 2000-04-25 The Perkin-Elmer Corporation Ribosomal RNA gene polymorphism based microorganism identification
EP1205559B1 (fr) * 1999-07-28 2009-06-24 Kaken Pharmaceutical Co., Ltd. Methode de detection des micro-organismes pathogenes et des agents antimicrobiens, methode d'evaluation de l'effet medicamenteux des agents antimicrobiens, et agents antimicrobiens
WO2004015141A2 (fr) * 2002-08-08 2004-02-19 University Of Lausanne Nouveaux outils moleculaires destines a l'evaluation rapide de la presence et de la viabilite de micro-organismes et procedes d'utilisation de ces outils
US20090275063A1 (en) * 2005-05-03 2009-11-05 Marc Green Distinguishing cells in a sample by inactivating extracellular enzyme before releasing intracellular enzyme
WO2009007719A2 (fr) * 2007-07-09 2009-01-15 Iseao Technologies Limited Détection améliorée de micro-organismes

Also Published As

Publication number Publication date
EP2558600A4 (fr) 2014-04-30
SG184563A1 (en) 2012-11-29
CN103328652B (zh) 2016-12-28
CA2796676C (fr) 2020-07-14
EP2558600B1 (fr) 2017-10-04
JP2013537399A (ja) 2013-10-03
ZA201208522B (en) 2014-08-27
EP2558600B2 (fr) 2020-06-17
AU2011239587A1 (en) 2012-11-08
EP3284831B1 (fr) 2019-07-24
EP2558600A2 (fr) 2013-02-20
ES2644258T3 (es) 2017-11-28
ES2644258T5 (es) 2021-02-02
AU2011239587B2 (en) 2016-03-10
NZ701866A (en) 2016-07-29
CA2796676A1 (fr) 2011-10-20
KR20130094714A (ko) 2013-08-26
JP2017148067A (ja) 2017-08-31
US20180230551A1 (en) 2018-08-16
ES2906061T3 (es) 2022-04-13
PH12016500300A1 (en) 2019-02-04
KR102068136B1 (ko) 2020-01-20
EP3284831A1 (fr) 2018-02-21
US10876173B2 (en) 2020-12-29
AP2012006530A0 (en) 2012-10-31
AP3635A (fr) 2016-03-09
ES2739806T3 (es) 2020-02-04
US20170321287A1 (en) 2017-11-09
EP3617320A1 (fr) 2020-03-04
AU2016203878A1 (en) 2016-06-30
KR20180086526A (ko) 2018-07-31
US10870894B2 (en) 2020-12-22
PH12012502065A1 (en) 2019-07-10
US20130196318A1 (en) 2013-08-01
MY161567A (en) 2017-04-28
JP6603956B2 (ja) 2019-11-13
CN103328652A (zh) 2013-09-25
SG10201502900PA (en) 2015-06-29
NZ720529A (en) 2017-01-27
EP3617320B1 (fr) 2022-01-26
EA201291001A1 (ru) 2013-09-30
WO2011130584A2 (fr) 2011-10-20

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