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WO2011129719A3 - Способ получения кардиомиоцитарной матрицы - Google Patents

Способ получения кардиомиоцитарной матрицы Download PDF

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Publication number
WO2011129719A3
WO2011129719A3 PCT/RU2011/000214 RU2011000214W WO2011129719A3 WO 2011129719 A3 WO2011129719 A3 WO 2011129719A3 RU 2011000214 W RU2011000214 W RU 2011000214W WO 2011129719 A3 WO2011129719 A3 WO 2011129719A3
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solution
stem cells
medium
cardiomyocytes
differentiation
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French (fr)
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WO2011129719A2 (ru
Inventor
Артем Валерьевич ЕРЕМЕЕВ
Анатолий Васильевич СВЕТЛАКОВ
Игорь Николаевич БОЛЬШАКОВ
Юлия Игоревна ШЕИНА
Алексей Михайлович ПОЛСТЯНОЙ
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Gosudarstvennoye Obrazovatel'noye Uchrezhdeniye Vysshyego Prophyessional'nogo Obrazovanija "krasnojarskij Gosudarstvyennyj Myeditsinskij Univyersitet Imyeni Prophyessora V Ph Vojno-Jasyenetskogo Ministyerstva Zdravookhranyenija I Sotsial'nogo Razvitija Rossijskoj Phyedyeratsii"
Original Assignee
Gosudarstvennoye Obrazovatel'noye Uchrezhdeniye Vysshyego Prophyessional'nogo Obrazovanija "krasnojarskij Gosudarstvyennyj Myeditsinskij Univyersitet Imyeni Prophyessora V Ph Vojno-Jasyenetskogo Ministyerstva Zdravookhranyenija I Sotsial'nogo Razvitija Rossijskoj Phyedyeratsii"
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Application filed by Gosudarstvennoye Obrazovatel'noye Uchrezhdeniye Vysshyego Prophyessional'nogo Obrazovanija "krasnojarskij Gosudarstvyennyj Myeditsinskij Univyersitet Imyeni Prophyessora V Ph Vojno-Jasyenetskogo Ministyerstva Zdravookhranyenija I Sotsial'nogo Razvitija Rossijskoj Phyedyeratsii" filed Critical Gosudarstvennoye Obrazovatel'noye Uchrezhdeniye Vysshyego Prophyessional'nogo Obrazovanija "krasnojarskij Gosudarstvyennyj Myeditsinskij Univyersitet Imyeni Prophyessora V Ph Vojno-Jasyenetskogo Ministyerstva Zdravookhranyenija I Sotsial'nogo Razvitija Rossijskoj Phyedyeratsii"
Priority to EP11769150.1A priority Critical patent/EP2559755A4/de
Publication of WO2011129719A2 publication Critical patent/WO2011129719A2/ru
Publication of WO2011129719A3 publication Critical patent/WO2011129719A3/ru
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/06—Anti-neoplasic drugs, anti-retroviral drugs, e.g. azacytidine, cyclophosphamide
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/065—Modulators of histone acetylation
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/72—Chitin, chitosan

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
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  • Wood Science & Technology (AREA)
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  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Heart & Thoracic Surgery (AREA)
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  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract

Изобретение относится к биологии и медицине, а именно: к кардиологии и кардиохирургии, к клеточной биотехнологии и биоинженерии, трансплантологии, и может быть использовано для культивирования, пролиферации и дифференцировки стволовых клеток в кардиомиоциты с целью трансплантации. Задача изобретения - повысить качество и стабильность культивирования, пролиферативную активность стволовых плюрипотентных клеток с их дифференцировкой в кардиомиоциты, исключить иммуногенность микроокружения клеток и обеспечить получение клеточной матрицы, пригодной для прямой трансплантации. Задачу достигают за счет того, что биомассу эмбриональных стволовых клеток человека безфидерной линии hESKM-05 наращивают с использованием основной среды mTeSR во флаконах, покрытых матригелем, и пассируют с помощью 0,05% раствора деспазы, затем для образования из эмбриональных стволовых клеток эмбриоидных телец клетки переносят с помощью деспазы в среду коДМЕМ с добавлением 10% заменителя сыворотки SR, 100 мкг/мл канамицина сульфата, 1мМ раствора L- глутамина и 1 мМ раствора незаменимых аминокислот, а также 2мкг/мл раствора 5- аза-2-дезоксицитидина или 2мкМ раствора бутирата натрия и стимулируют в течение 3 суток, при этом эмбриоидные тельца в дальнейшем переносят на подготовленную коллаген-хитозановую матрицу в виде губки, пленки, микросфер, геля или волокон и культивируют в питательной среде коДМЕМ с добавлением 1 мМ раствора незаменимых аминокислот, 1 мМ раствора L-глутамина, 10% заменителя сыворотки SR, 10-7М ретиноевой кислоты и 10 нг/мл аскорбиновой кислоты со сменой среды каждые трое суток. Использование способа повышает качество и стабильность культивирования, пролиферативную активность стволовых плюрипотентных клеток с их дифференцировкой в кардиомиоциты, исключает иммуногенность микроокружения клеток и обеспечивает получение кардиомиоцитарной матрицы, пригодной для прямой трансплантации.
PCT/RU2011/000214 2010-04-12 2011-04-01 Способ получения кардиомиоцитарной матрицы Ceased WO2011129719A2 (ru)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP11769150.1A EP2559755A4 (de) 2010-04-12 2011-04-01 Verfahren zur herstellung einer kardiomyozytenmatrix

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2010114380/10A RU2430159C1 (ru) 2010-04-12 2010-04-12 Способ получения кардиомиоцитарной матрицы
RU2011114380 2010-04-12

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WO2011129719A2 WO2011129719A2 (ru) 2011-10-20
WO2011129719A3 true WO2011129719A3 (ru) 2012-02-16

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PCT/RU2011/000214 Ceased WO2011129719A2 (ru) 2010-04-12 2011-04-01 Способ получения кардиомиоцитарной матрицы

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EP (1) EP2559755A4 (ru)
RU (1) RU2430159C1 (ru)
WO (1) WO2011129719A2 (ru)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2008131742A (ru) * 2008-07-31 2010-02-10 Государственное Образовательное Учреждение Высшего Профессионального Образования "Красноярская Государственная Медицинская Академи Способ культивирования эмбриональных клеток и получения клеточных матриц

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6699287B2 (en) * 1998-09-24 2004-03-02 Korea Atomic Energy Research Institute Dermal scaffold using alkaline pre-treated chitosan matrix or alkaline pre-treated chitosan and alkaline pre-treated collagen mixed matrix
US7732199B2 (en) * 2001-07-12 2010-06-08 Geron Corporation Process for making transplantable cardiomyocytes from human embryonic stem cells
RU2254145C1 (ru) 2003-10-14 2005-06-20 "Красноярская государственная медицинская академия Министерства здравоохранения Российской Федерации" (ГОУ ВПО КРАСГМА МИНЗДРАВА РОССИИ) Раневое покрытие на основе коллаген-хитозанового комплекса
RU2252787C1 (ru) 2003-12-16 2005-05-27 ГОУ ВПО "Красноярская государственная медицинская академия" Министерства здравоохранения РФ Способ получения искусственной матрицы кожи
WO2005093047A2 (en) 2004-03-24 2005-10-06 Universite De Geneve 3d-cardiac tissue engineering for the cell therapy of heart failure

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2008131742A (ru) * 2008-07-31 2010-02-10 Государственное Образовательное Учреждение Высшего Профессионального Образования "Красноярская Государственная Медицинская Академи Способ культивирования эмбриональных клеток и получения клеточных матриц

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHUNHUI XU ET AL.: "Characterization and Enrichment of Cardiomyocytes Derived From Human Embryonic Stem Cells", CIRCULATION RESEARCH, vol. 91, no. 6, 2002, pages 501 - 508, XP002282072 *
E LL. ET AL.: "Enrichment of cardiomyocytes derived from mouse embryonic stem cells", J HEART LUNG TRANSPLANT, vol. 25, no. 6, 2006, pages 664 - 674, XP025015229 *
EREMEEV A.V. ET AL.: "Derivation of a Novel Human Embryonic Stem Cell Line under Serum-Free and Feeder-Free Conditions", DOKLADY BIOLOGICAL SCIENCES, vol. 426, no. 2, 2009, pages 293 - 294, XP009152216 *
See also references of EP2559755A4 *
WOBUS A.M. ET AL.: "Retinoic acid accelerates embryonic stem cell-derived cardiac differentiation and enhances development of ventricular cardiomyocytes", J MOL CELL CARDIOL, vol. 29, no. 6, 1997, pages 1525 - 1539, XP002179466 *

Also Published As

Publication number Publication date
WO2011129719A2 (ru) 2011-10-20
RU2430159C1 (ru) 2011-09-27
EP2559755A2 (de) 2013-02-20
EP2559755A4 (de) 2013-08-14

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