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WO2011117844A3 - Method for evaluation of multiple antibodies or multiple antigens in a single sample - Google Patents

Method for evaluation of multiple antibodies or multiple antigens in a single sample Download PDF

Info

Publication number
WO2011117844A3
WO2011117844A3 PCT/IB2011/051270 IB2011051270W WO2011117844A3 WO 2011117844 A3 WO2011117844 A3 WO 2011117844A3 IB 2011051270 W IB2011051270 W IB 2011051270W WO 2011117844 A3 WO2011117844 A3 WO 2011117844A3
Authority
WO
WIPO (PCT)
Prior art keywords
antigens
single sample
antibodies
evaluation
multiple antibodies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2011/051270
Other languages
French (fr)
Other versions
WO2011117844A2 (en
Inventor
Subhash V. Kapre
Manoj Kumar Chhikara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Serum Institute of India Pvt Ltd
Original Assignee
Serum Institute of India Pvt Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Serum Institute of India Pvt Ltd filed Critical Serum Institute of India Pvt Ltd
Publication of WO2011117844A2 publication Critical patent/WO2011117844A2/en
Publication of WO2011117844A3 publication Critical patent/WO2011117844A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/315—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Streptococcus (G), e.g. Enterococci
    • G01N2333/3156—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae [Pneumococcus]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/02—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates involving antibodies to sugar part of glycoproteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A multiplex assay for simultaneous detection of multiple antibodies or multiple antigens in single sample, wherein carboxylated polystyrene microsphere sets are conjugated with a defined candidate antigens by an optimized conjugation method and are mixed to enable the detection of multiple antibodies or multiple antigens, respectively. The multiplex assay of the invention is utilized for quantitation of antibody or antigen wherein minimum heterologous inhibition(cross-reactivity) of less than 20% for all serotypes and homologous inhibition greater than 80% can be obtained, wherein the polysaccharide coupled microspheres have been prepared by an optimal method without altering the critical epitopes of polysaccharide.
PCT/IB2011/051270 2010-03-26 2011-03-25 Method for evaluation of multiple antibodies or multiple antigens in a single sample Ceased WO2011117844A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN863MU2010 2010-03-26
IN863/MUM/2010 2010-03-26

Publications (2)

Publication Number Publication Date
WO2011117844A2 WO2011117844A2 (en) 2011-09-29
WO2011117844A3 true WO2011117844A3 (en) 2012-07-05

Family

ID=44673711

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2011/051270 Ceased WO2011117844A2 (en) 2010-03-26 2011-03-25 Method for evaluation of multiple antibodies or multiple antigens in a single sample

Country Status (1)

Country Link
WO (1) WO2011117844A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109738644B (en) * 2018-12-24 2022-02-18 恩碧乐(杭州)生物科技有限公司 Anti-mullerian hormone immune turbidimetry quantitative detection reagent
CN111205854B (en) * 2020-02-26 2021-06-08 成都以邦医药科技有限公司 Quantum dot-embedded high-molecular fluorescent composite microsphere and preparation method and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030073073A1 (en) * 2001-10-11 2003-04-17 Wondu Wolde-Mariam Method for simultaneous detection of multiple microbial antigens in biological specimens from mastitic animals
US20050118199A1 (en) * 2003-10-07 2005-06-02 Esser Mark T. Process for covalently conjugating polysaccharides to microspheres or biomolecules
US20090169636A1 (en) * 2006-02-24 2009-07-02 Derek O' Hagan Microparticles containing biodegradable polymer and cationic polysaccharide for use in immunogenic compositions
US20100062416A1 (en) * 2006-10-24 2010-03-11 Aldert Anthonie Bergwerff Immobilisation and application of antigenic carbohydrates to detect infective micro-organisms

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030073073A1 (en) * 2001-10-11 2003-04-17 Wondu Wolde-Mariam Method for simultaneous detection of multiple microbial antigens in biological specimens from mastitic animals
US20050118199A1 (en) * 2003-10-07 2005-06-02 Esser Mark T. Process for covalently conjugating polysaccharides to microspheres or biomolecules
US20090169636A1 (en) * 2006-02-24 2009-07-02 Derek O' Hagan Microparticles containing biodegradable polymer and cationic polysaccharide for use in immunogenic compositions
US20100062416A1 (en) * 2006-10-24 2010-03-11 Aldert Anthonie Bergwerff Immobilisation and application of antigenic carbohydrates to detect infective micro-organisms

Also Published As

Publication number Publication date
WO2011117844A2 (en) 2011-09-29

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