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WO2011117414A3 - Device for carrying out pcr, method for producing a device for carrying out pcr and pcr method - Google Patents

Device for carrying out pcr, method for producing a device for carrying out pcr and pcr method Download PDF

Info

Publication number
WO2011117414A3
WO2011117414A3 PCT/EP2011/054672 EP2011054672W WO2011117414A3 WO 2011117414 A3 WO2011117414 A3 WO 2011117414A3 EP 2011054672 W EP2011054672 W EP 2011054672W WO 2011117414 A3 WO2011117414 A3 WO 2011117414A3
Authority
WO
WIPO (PCT)
Prior art keywords
holder
carrying
sample
sample vessel
out pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2011/054672
Other languages
German (de)
French (fr)
Other versions
WO2011117414A2 (en
Inventor
Joachim Nurnus
Axel Schubert
Fritz Volkert
Daniel Hoeferlin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Micropelt GmbH
Original Assignee
Micropelt GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Micropelt GmbH filed Critical Micropelt GmbH
Publication of WO2011117414A2 publication Critical patent/WO2011117414A2/en
Publication of WO2011117414A3 publication Critical patent/WO2011117414A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/147—Employing temperature sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Re-Forming, After-Treatment, Cutting And Transporting Of Glass Products (AREA)

Abstract

The invention relates to a device for carrying out PCR, comprising a sample holder (2) for holding sample vessels (3), wherein the sample holder (2) has a plurality of holder openings (21), in each of which a sample vessel or an insert (3) for holding a sample vessel can be arranged; a plurality of Peltier elements (5), a plurality of temperature sensors (4) for determining the temperature of a sample present in a sample vessel (3), wherein each of the holder openings (21) is assigned at least one of the temperature sensors (4); and a plurality of restoring force generating means, wherein each of the holder openings (21) is assigned at least one restoring force generating means which interacts with the temperature sensor (4) assigned to the holder opening (21) in such a manner that said means holds the temperature sensor (4) in thermal contact with a sample vessel arranged in the holder opening (21) or with an insert for holding a sample vessel, wherein at least one of the restoring force generating means has an elastic element (6) on which the temperature sensor (4) is arranged, wherein the elastic element (6) projects beyond an edge of the holder opening (21) or into the holder opening (21), so it is bent down and the temperature sensor (4) is in this manner prestressed against the sample vessel or the insert (3) after a sample vessel or an insert (3) is arranged in the associated holder opening (21). The invention further relates to a method for producing a device for carrying out PCR and to a method for carrying out PCR.
PCT/EP2011/054672 2010-03-26 2011-03-28 Device for carrying out pcr, method for producing a device for carrying out pcr and pcr method Ceased WO2011117414A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102010003365.0 2010-03-26
DE201010003365 DE102010003365A1 (en) 2010-03-26 2010-03-26 Apparatus for carrying out the PCR and PCR methods

Publications (2)

Publication Number Publication Date
WO2011117414A2 WO2011117414A2 (en) 2011-09-29
WO2011117414A3 true WO2011117414A3 (en) 2011-11-17

Family

ID=44115289

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/054672 Ceased WO2011117414A2 (en) 2010-03-26 2011-03-28 Device for carrying out pcr, method for producing a device for carrying out pcr and pcr method

Country Status (2)

Country Link
DE (1) DE102010003365A1 (en)
WO (1) WO2011117414A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2790474B1 (en) 2013-04-09 2016-03-16 Harman Becker Automotive Systems GmbH Thermoelectric cooler/heater integrated in printed circuit board
EP4378585A1 (en) 2022-11-29 2024-06-05 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Device and method for carrying out a rapid and precise temperature control of chemical substances for synthesis, homogenisation and diagnostics

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5601141A (en) * 1992-10-13 1997-02-11 Intelligent Automation Systems, Inc. High throughput thermal cycler
US20010019826A1 (en) * 1998-05-01 2001-09-06 Gen-Probe Incorporated Automated diagnostic analyzer and method
US20020086417A1 (en) * 2000-12-29 2002-07-04 Shuqi Chen Sample processing device and method
US6633785B1 (en) * 1999-08-31 2003-10-14 Kabushiki Kaisha Toshiba Thermal cycler and DNA amplifier method
WO2004105947A2 (en) * 2003-05-23 2004-12-09 Bio-Rad Laboratories, Inc. Localized temperature control for spatial arrays of reaction media
EP2060324A1 (en) * 2007-11-13 2009-05-20 F.Hoffmann-La Roche Ag Thermal block unit
WO2009111475A2 (en) * 2008-03-03 2009-09-11 Heatflow Technologies, Inc. Heat flow polymerase chain reaction systems and methods
WO2010012494A1 (en) * 2008-08-01 2010-02-04 Eppendorf Ag Heating/cooling device having opportunity for testing and method for testing a heating/cooling device

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6558947B1 (en) * 1997-09-26 2003-05-06 Applied Chemical & Engineering Systems, Inc. Thermal cycler
ES2183563T3 (en) * 1998-05-19 2003-03-16 Cepheid MULTICHANNEL OPTICAL DETECTION SYSTEM.
DE19845104A1 (en) 1998-09-30 2000-04-06 Siemens Ag Method of manufacturing a thermoelectric converter
TW463184B (en) * 1999-04-09 2001-11-11 Murata Manufacturing Co Temperature sensor, method of producing same and method of mounting same to a circuit board

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5601141A (en) * 1992-10-13 1997-02-11 Intelligent Automation Systems, Inc. High throughput thermal cycler
US20010019826A1 (en) * 1998-05-01 2001-09-06 Gen-Probe Incorporated Automated diagnostic analyzer and method
US6633785B1 (en) * 1999-08-31 2003-10-14 Kabushiki Kaisha Toshiba Thermal cycler and DNA amplifier method
US20020086417A1 (en) * 2000-12-29 2002-07-04 Shuqi Chen Sample processing device and method
WO2004105947A2 (en) * 2003-05-23 2004-12-09 Bio-Rad Laboratories, Inc. Localized temperature control for spatial arrays of reaction media
EP2060324A1 (en) * 2007-11-13 2009-05-20 F.Hoffmann-La Roche Ag Thermal block unit
WO2009111475A2 (en) * 2008-03-03 2009-09-11 Heatflow Technologies, Inc. Heat flow polymerase chain reaction systems and methods
WO2010012494A1 (en) * 2008-08-01 2010-02-04 Eppendorf Ag Heating/cooling device having opportunity for testing and method for testing a heating/cooling device

Also Published As

Publication number Publication date
DE102010003365A1 (en) 2011-09-29
WO2011117414A2 (en) 2011-09-29

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