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WO2011100286A3 - Compositions et méthodes de production d'une cellule mésodermique différenciée - Google Patents

Compositions et méthodes de production d'une cellule mésodermique différenciée Download PDF

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Publication number
WO2011100286A3
WO2011100286A3 PCT/US2011/024142 US2011024142W WO2011100286A3 WO 2011100286 A3 WO2011100286 A3 WO 2011100286A3 US 2011024142 W US2011024142 W US 2011024142W WO 2011100286 A3 WO2011100286 A3 WO 2011100286A3
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WIPO (PCT)
Prior art keywords
methods
compositions
generating
differentiated mesodermal
differentiated
Prior art date
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Ceased
Application number
PCT/US2011/024142
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English (en)
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WO2011100286A2 (fr
Inventor
Elias T. Zambidis
Paul W. Burridge
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Johns Hopkins University
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Johns Hopkins University
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Priority to US13/577,944 priority Critical patent/US20130136721A1/en
Publication of WO2011100286A2 publication Critical patent/WO2011100286A2/fr
Publication of WO2011100286A3 publication Critical patent/WO2011100286A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cardiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Vascular Medicine (AREA)
  • Immunology (AREA)
  • Rheumatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention a pour objet un système de culture, des composants de système de culture et des méthodes de culture qui sont utiles pour la production rapide et fiable de cellules mésodermiques différenciées, y compris des myocytes cardiaques, à partir de cellules souches, telles que des cellules souches embryonnaires humaines et des cellules souches pluripotentes induites humaines, y compris des myocytes cardiaques différenciés.
PCT/US2011/024142 2010-02-09 2011-02-09 Compositions et méthodes de production d'une cellule mésodermique différenciée Ceased WO2011100286A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/577,944 US20130136721A1 (en) 2010-02-09 2011-02-09 Compositions and Methods of Generating a Differentiated Mesodermal Cell

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US30271410P 2010-02-09 2010-02-09
US61/302,714 2010-02-09
US201061426292P 2010-12-22 2010-12-22
US61/426,292 2010-12-22

Publications (2)

Publication Number Publication Date
WO2011100286A2 WO2011100286A2 (fr) 2011-08-18
WO2011100286A3 true WO2011100286A3 (fr) 2011-12-15

Family

ID=44368406

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PCT/US2011/024142 Ceased WO2011100286A2 (fr) 2010-02-09 2011-02-09 Compositions et méthodes de production d'une cellule mésodermique différenciée

Country Status (2)

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US (1) US20130136721A1 (fr)
WO (1) WO2011100286A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101390613B1 (ko) 2012-03-16 2014-04-29 아주대학교산학협력단 Ｓｅｌｅｎｉｕｍ을 이용한 인간 만능줄기세포의 혈액전구세포, 혈관전구세포, 내피세포 및 평활근세포로의 분화방법
CA3135208A1 (fr) * 2013-06-11 2014-12-18 Ncardia B.V. Compositions de milieu de culture pour la maturation de cardiomyocytes derives de cellules souches mammaliennes pluripotentes.
AU2014303443B2 (en) * 2013-08-06 2020-11-12 Riken Method for producing anterior eye segment tissue
CN106164257B (zh) 2014-03-31 2021-03-09 味之素株式会社 干细胞用培养基
JP7181534B2 (ja) 2017-03-28 2022-12-01 味の素株式会社 未分化維持培地添加剤
JP2020532992A (ja) * 2017-09-07 2020-11-19 ステモニックス インコーポレイティド 胚体内胚葉へのヒト幹細胞の効率的な分化
CN111206017B (zh) * 2019-04-30 2022-02-18 浙江大学 一种干细胞无血清培养基及其应用
CN110923196B (zh) * 2019-12-03 2021-07-09 广州赛莱拉干细胞科技股份有限公司 无血清培养基及其制备方法和间充质干细胞的培养方法
US20240407955A1 (en) 2021-09-30 2024-12-12 Warszawski Uniwersytet Medyczny Dressing for treating hard-to-heal wounds and a process for the manufacture thereof
CN115089716B (zh) * 2022-07-12 2023-04-21 齐齐哈尔医学院 Bmp4作为作用靶点在制备治疗糖尿病心肌病药物中的应用
CN115466717A (zh) * 2022-09-20 2022-12-13 昆明理工大学 一种非人灵长类胚胎体外培养试剂盒和体外培养方法
CN115851584A (zh) * 2022-12-21 2023-03-28 昆明医科大学第一附属医院 RNA结合蛋白Rbm24基因在心肌病和心律失常中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040121464A1 (en) * 2002-09-30 2004-06-24 Rathjen Peter David Method for the preparation of cells of mesodermal lineage
WO2008056166A2 (fr) * 2006-11-09 2008-05-15 Cambridge Enterprise Limited Différenciation de cellules pluripotentes en cellules progénitrices de couches germinales primaires

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1791952A4 (fr) * 2004-08-13 2008-06-11 Univ Georgia Res Found Compositions et procedes d'auto-renouvellement et de differentiation dans des cellules souches embryonnaires humaines
US9029147B2 (en) * 2007-06-15 2015-05-12 Massachusetts Institute Of Technology Methods and compositions for enhanced differentiation from embryonic stem cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040121464A1 (en) * 2002-09-30 2004-06-24 Rathjen Peter David Method for the preparation of cells of mesodermal lineage
WO2008056166A2 (fr) * 2006-11-09 2008-05-15 Cambridge Enterprise Limited Différenciation de cellules pluripotentes en cellules progénitrices de couches germinales primaires

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
VALLIER ET AL.: "Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways", PLOS ONE, vol. 4, no. 6, 30 June 2009 (2009-06-30), pages E6082-1 - 13 *

Also Published As

Publication number Publication date
US20130136721A1 (en) 2013-05-30
WO2011100286A2 (fr) 2011-08-18

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