[go: up one dir, main page]

WO2011146928A3 - Methods for calibrating protein levels in cells and tissue specimens - Google Patents

Methods for calibrating protein levels in cells and tissue specimens Download PDF

Info

Publication number
WO2011146928A3
WO2011146928A3 PCT/US2011/037574 US2011037574W WO2011146928A3 WO 2011146928 A3 WO2011146928 A3 WO 2011146928A3 US 2011037574 W US2011037574 W US 2011037574W WO 2011146928 A3 WO2011146928 A3 WO 2011146928A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
expression
calibrating
methods
protein levels
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/037574
Other languages
French (fr)
Other versions
WO2011146928A2 (en
Inventor
Brian Ward
William E. Pierceall
Stella Quan
Yan Chen
Xiaozhe Wang
Lakshmi P. Alaparthi
Kam Marie Sprott
Hua Chang
David T. Weaver
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
On-Q-ity
On Q ity
Original Assignee
On-Q-ity
On Q ity
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by On-Q-ity, On Q ity filed Critical On-Q-ity
Publication of WO2011146928A2 publication Critical patent/WO2011146928A2/en
Publication of WO2011146928A3 publication Critical patent/WO2011146928A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hospice & Palliative Care (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method is described for the use of an internal calibrator marker for normalization of quantifiable protein detection in cells. This internal calibrator will ostensibly serve as an enumerator of epitope immune-integrity. Optimally, it will be a robust biomarker analyte of the same class (i.e protein) to that for which it is being held as a control, with consistent levels of expression across tissues but with a dynamic range commensurate with assay and treatment conditions. While stable expression in a biological context is a requirement, it will exhibit a suitable dynamic level of expression due to technical variation relative to a test marker to which it will serve as a surrogate.
PCT/US2011/037574 2010-05-21 2011-05-23 Methods for calibrating protein levels in cells and tissue specimens Ceased WO2011146928A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34696810P 2010-05-21 2010-05-21
US61/346,968 2010-05-21

Publications (2)

Publication Number Publication Date
WO2011146928A2 WO2011146928A2 (en) 2011-11-24
WO2011146928A3 true WO2011146928A3 (en) 2012-04-05

Family

ID=44992379

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/037574 Ceased WO2011146928A2 (en) 2010-05-21 2011-05-23 Methods for calibrating protein levels in cells and tissue specimens

Country Status (1)

Country Link
WO (1) WO2011146928A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105548137A (en) * 2015-12-25 2016-05-04 中国科学院广州能源研究所 High-pressure cooling-heating table device for in-situ observation of aquo-complex microscopic reaction kinetics process and application method
WO2017151989A1 (en) * 2016-03-02 2017-09-08 Flagship Biosciences, Inc. Method for assigning tissue normalization factors for digital image analysis
WO2024215582A1 (en) * 2023-04-14 2024-10-17 Ventana Medical Systems, Inc. Ground truth of signal aggregate quantification

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6077665A (en) * 1996-05-07 2000-06-20 The Board Of Trustees Of The Leland Stanford Junior University Rapid assay for infection in neonates
US20070225261A1 (en) * 2006-02-22 2007-09-27 Miller Guy M Side-chain variants of redox-active therapeutics for treatment of mitochondrial diseases and other conditions and modulation of energy biomarkers
US20080264152A1 (en) * 2002-08-06 2008-10-30 The Regents Of The University Of California Biomarker normalization
US20090062624A1 (en) * 2007-04-26 2009-03-05 Thomas Neville Methods and systems of delivering a probability of a medical condition
WO2010033508A1 (en) * 2008-09-16 2010-03-25 Historx, Inc. Reproducible quantification of biomarker expression

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6077665A (en) * 1996-05-07 2000-06-20 The Board Of Trustees Of The Leland Stanford Junior University Rapid assay for infection in neonates
US20080264152A1 (en) * 2002-08-06 2008-10-30 The Regents Of The University Of California Biomarker normalization
US20070225261A1 (en) * 2006-02-22 2007-09-27 Miller Guy M Side-chain variants of redox-active therapeutics for treatment of mitochondrial diseases and other conditions and modulation of energy biomarkers
US20090062624A1 (en) * 2007-04-26 2009-03-05 Thomas Neville Methods and systems of delivering a probability of a medical condition
WO2010033508A1 (en) * 2008-09-16 2010-03-25 Historx, Inc. Reproducible quantification of biomarker expression

Also Published As

Publication number Publication date
WO2011146928A2 (en) 2011-11-24

Similar Documents

Publication Publication Date Title
WO2014186761A3 (en) Methods for determining responsiveness to an anti-cd47 agent
BR112013003391A2 (en) pancreatic cancer biomarkers and their uses
WO2010096154A3 (en) Compositions and methods for diagnosis and prognosis of colorectal cancer
EP3102706A4 (en) Ultra-sensitive detection of extremely low level biological analytes using electrochemical signal amplification and biosensor
WO2011144718A3 (en) Methods and kits for diagnosing colorectal cancer
WO2012096545A3 (en) Novel pancreatic cancer biomarker using the characteristics of pancreatic cancer stem cells, and use thereof
NZ621733A (en) Lung cancer biomarkers and uses thereof
BR112012016119B1 (en) cell detection method in a sample, unit sample preparation and detection device and kit
WO2014170497A3 (en) Method for identifying the quantitative cellular composition in a biological sample
WO2011034935A3 (en) Transcription biomarkers of biological responses and methods
WO2012056236A3 (en) Analytical methods and arrays for use in the identification of agents inducing sensitization in human skin
WO2010105275A3 (en) Method to assess human allograft status from microrna expression levels
WO2012032345A3 (en) Biomarker signatures and uses thereof
BRPI1013896A2 (en) Yeast cell, and methods for producing an isoprenoid compound and for detecting in a biological sample the presence or absence of a genetically microbial cell.
EP2532748A3 (en) DNA aptamer specifically binding to human cardiac troponin I
WO2015017654A3 (en) Method of collecting and quantifying melanin in skin using adhesive
WO2012047009A3 (en) Diagnostic apparatus and diagnostic method using the same
Sipieter et al. From FRET imaging to practical methodology for kinase activity sensing in living cells
BR112013004278A2 (en) enzyme reagent inks for use in test strips having a predetermined calibration code
WO2009019600A3 (en) Modulation of endogenous ampk levels for the treatment of obesity
EP4461219A3 (en) Analyte level calibration using baseline analyte level
WO2012071344A3 (en) Nucleotide-based probes and methods for the detection and quantification of macromolecules and other analytes
BR112014031957A2 (en) bag3 as biochemical serum and tissue marker
WO2011146928A3 (en) Methods for calibrating protein levels in cells and tissue specimens
BR112014000219A2 (en) Assay device for detecting an active enzyme in a sample, method for determining the presence and levels of an active enzyme in a sample, kit, assay device, or method of use or use and use

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11784383

Country of ref document: EP

Kind code of ref document: A2