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WO2011031166A2 - Composition d'aliment - Google Patents

Composition d'aliment Download PDF

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Publication number
WO2011031166A2
WO2011031166A2 PCT/NO2010/000336 NO2010000336W WO2011031166A2 WO 2011031166 A2 WO2011031166 A2 WO 2011031166A2 NO 2010000336 W NO2010000336 W NO 2010000336W WO 2011031166 A2 WO2011031166 A2 WO 2011031166A2
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WO
WIPO (PCT)
Prior art keywords
fatty acids
feed composition
composition according
weight
feed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/NO2010/000336
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English (en)
Other versions
WO2011031166A3 (fr
Inventor
José Luis Gonzalez VECINO
Simon Wadsworth
Sigve Nordrum
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CHEMOFORMA Ltd
Aker Biomarine ASA
Cargill Innovation Center Dirdal
Original Assignee
CHEMOFORMA Ltd
Aker Biomarine ASA
EWOS Innovation AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CHEMOFORMA Ltd, Aker Biomarine ASA, EWOS Innovation AS filed Critical CHEMOFORMA Ltd
Priority to NO20120303A priority Critical patent/NO347704B1/no
Publication of WO2011031166A2 publication Critical patent/WO2011031166A2/fr
Publication of WO2011031166A3 publication Critical patent/WO2011031166A3/fr
Anticipated expiration legal-status Critical
Priority to NO20120594A priority patent/NO20120594A1/no
Priority to NO20231264A priority patent/NO348543B1/no
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Definitions

  • the present invention relates to a feed composition for fish comprising conventional feed ingredients such as proteins, lipids, vitamins, carbohydrates and minerals, a use of the feed composition as well as to a method for feeding of fish which are susceptible to a Heart and skeletal muscle inflammation infection.
  • Heart and skeletal muscle inflammation disease is a serious infectious disease of Atlantic salmon with a high potential for transmission from infected to healthy fish. Since the first occurrence in 1999, an increasing number of outbreaks of the disease have been registered. Thus, after 68 outbreaks registered in 2003 and 54 in 2004, the number has been increasing every year. In 2007 the disease was diagnosed at 162 seawater sites along the Norwegian coast and in 144 in 2008 ( Figure 1 ). These outbreaks are causing huge losses to the Norwegian salmon industry since on sites that are affected, very high numbers of fish (>90%) may suffer heart lesions months after the disease event, significantly reducing their growth potential. Most outbreaks are reported in January, June, July and October, in fish transferred to seawater. The disease has a great economical impact to the
  • HSMI Clinical signs of HSMI are typically reduced appetite and aberrant swimming behaviour. There are no obvious external signs, but at autopsy the heart appears pale, soft and flaccid. HSMI produces inflammation of the heart which results in death of heart cells. These damages can already be seen in early stages of the disease and can continue for several months. Mortalities increase with an increasing degree of heart inflammation. The inflammation and cell death can be spread to other organs and tissues such as the red skeletal muscle and the liver causing additional severe damages also to these organs.
  • HSMI SUBSTITUTE SHEET
  • the infectious nature of HSMI has been proven, as disease could be induced in healthy fish by injecting homogenized tissue from fish diagnosed with HSMI, and further be transferred to healthy cohabitant fish (Kongtorp et ai, 2004. Heart and skeletal muscle inflammation in Atlantic salmon, Salmo salar L.: a new infectious disease. Journal of Fish Disease: Vol. 27(6), p. 351-358).
  • HSMI is caused by a virus, which has been isolated (Eliassen et al., 2004. Isolation of heart and skeletal muscleinflammation virus (HSMIV) from Salmon. The 6th International Symposium on Viruses of Lower Vertebrates, Poster 19).
  • An object of the present invention is therefore to find new strategies and treatments which can be used to positively affect the disease development in HSMI-infected fish as well as in fish susceptible to the infection.
  • a first aspect of the present invention relates to a feed composition for fish for the prevention and/or treatment of Heart and skeletal muscle inflammation disease comprising conventional feed ingredients such as proteins, lipids, vitamins, carbohydrates and minerals, characterized in that more than 20 % of the total fatty acids are n-3 fatty acids.
  • more than 23 % of the total fatty acids are n-3 fatty acids, and more preferable more than 24 % of the total fatty acids are n-3 fatty acids.
  • eicosapentaenoic acid 20:5 n-3 EPA
  • the content of eicosapentaenoic acid 20:5 n-3 is more than about 8 % of the total fatty acids, more preferably than 9 %, most preferably more than about 10%, such as about 10.2%.
  • a preferred embodiment contains the amounts of EPA indicated in table 1.
  • the proteins account for more than 50 % (by weight) and the lipids account for less than 20 % (by weight) of the feed composition. More preferable, the proteins account for more than 52 % (by weight) and the lipids account for about 18 % (by weight) of the feed composition.
  • a preferred embodiment relates to a feed composition for salmonids wherein the composition has a reduced content of lipids and an increased content of proteins when compared to commercial feed compositions used for salmonids at the same age, size and/or developmental stage.
  • n-6 fatty acids less than 11 % of the total fatty acids, preferably in the range of 7-11 %, most preferably about 10%.
  • n-3 to n-6 ratio fatty acids is higher than 1.6, preferably more than 2.2, most preferably between 2.3 and 3.4. In a preferred embodiment is a portion of said lipids South American Fish oil.
  • the feed composition contains less than 0.5 %, preferably 0.2 % by weight of the composition of added vegetable oil.
  • lipid added as a hydrolysate.
  • a preferred embodiment comprises hydrolysed phospholipids, preferably about 0.1- 0.2 % by weight of the composition, most preferably more than 0.2 % by weight of the composition.
  • a preferred embodiment comprises krill meal, preferably about 5 % by weight, most preferably more than 5 % by weight of the diet.
  • a preferred embodiment comprises nucleotides and/or manna-oligosaccharides and/or anti-inflammatory compounds such as flavonoids.
  • a preferred embodiment relates to a feed composition, wherein the proteins account for more than 50 % (by weight) of the composition, wherein the lipids account for less than 20 % (by weight), wherein more than 20 % of the total fatty acids are n-3 fatty acids. More preferable, the proteins account for more than 52 % (by weight) of the composition, and wherein the lipids account for less than 18 % (by weight), preferably for 18 % of the diet, wherein more than 23 % of the total fatty acids are n- 3 fatty acids.
  • a preferred embodiment relates to a feed composition substantially as indicated for the anti-HSMI diet 1 or for the anti HSMI diet 2 in table 1 or table 2.
  • a second aspect of the present invention relates to a feed composition as indicated above for the manufacturing of a pharmaceutical composition and/or nutritional composition and/or functional feed for the prophylaxis and/or treatment of Heart and skeletal muscle inflammation disease.
  • a preferred embodiment relates to prevention and reducing the symptoms related to Heart and skeletal muscle inflammation disease in fish.
  • the fish a salmonid, flat fish or any other fish species suitable for aquaculture preferably the species Atlantic salmon (Salmo salar).
  • Atlantic salmon Atlantic salmon
  • a third aspect of the present invention relates to a method for feeding of fish which are susceptible to a Heart and skeletal muscle inflammation infection characterized in that a composition as indicted above is fed to the fish in the period previous to the challenge by an infection, during the infection or after the fish has been infected.
  • the composition is fed for a period of at least 8 weeks pre infection, more preferably of 10-16 weeks, most preferably of 16-20 weeks and 8-20 weeks post infection.
  • a fourth aspect of the present invention relates to a feed composition for fish comprising conventional feed ingredients such as proteins, lipids, vitamins, carbohydrates and minerals, characterized in that more than 20 % of the total fatty acids are n-3 fatty acids.
  • more than 23 % of the total fatty acids are n-3 fatty acids.
  • more than 24 % of the total fatty acids are n-3 fatty acids.
  • the content of eicosapentaenoic acid 20:5 n-3 is more than about 7 % of the total fatty acids. More preferable, the content of eicosapentaenoic acid 20:5 n-3 (EPA) is more than about 8 % of the total fatty acids, more preferably than 9 %, most preferably more than about 10%, such as about 10.2%.
  • the proteinrlipid ratio (w/w) in the feed is higher than 2, preferably higher than 2.7 or the proteins account for more than 50 % (by weight) and the lipids account for less than 20 % (by weight) of the feed composition, more preferable wherein the proteins account for more than 52 % (by weight) and the lipids account for about 18 % (by weight) of the feed composition.
  • the content of n-6 fatty acids are less than 11 % of the total fatty acids, preferably in the range of 7-11 %, most preferably about 10%.
  • n-3 fatty acids are more than 23% and the level of n-6 fatty acids are less than 11 % of the total fatty acids.
  • ration of n-3 to n-6 ratio fatty acids is higher than 1.6, preferably more than 2.2, most preferably between 2.3 and 3.4.
  • lipids South American Fish oil In a preferred embodiment is a portion of said lipids South American Fish oil.
  • the content of added vegetable oil is less than 0.5 %, preferably 0.2 % by weight of the composition, and that the oil is preferably added as a hydrolysate.
  • the composition comprises hydrolysed phospholipids, preferably about 0.1-0.2 % by weight of the diet, most preferably more than 0.2 % by weight of the diet.
  • the composition comprises krill meal, preferably about 5 % by weight, most preferably more than 5 % by weight of the diet.
  • the feed further comprises nucleotides and/or manna-oligosaccharides and/or anti-inflammatory compounds such as flavonoids.
  • the proteins account for more than 50 % (by weight) of the composition, wherein the lipids account for less than 20 % (by weight), wherein more than 20 % of the total fatty acids are n-3 fatty acids, and more preferable the proteins account for more than 52 % (by weight) of the composition, and wherein the lipids account for less than 18 % (by weight), preferably for 18 % of the diet, wherein more than 23 % of the total fatty acids are n-3 fatty acids.
  • a fifth aspect of the present invention relates to a feed composition for fish comprising conventional feed ingredients such as proteins, lipids, vitamins, carbohydrates and minerals characterized in that the protein:lipid ratio (w/w) in the feed is higher than 2, preferably higher than 2.7 to prevent or reduce the symptoms related to Heart and skeletal muscle inflammation disease in the fish.
  • the protein:lipid ratio (w/w) in the feed is higher than 2.7
  • proteins account for more than 50 % (by weight) of the diet, preferably more than 52 % and the comprised lipids account for less than 20 % (by weight), preferably for 18 % of the diet.
  • more than 20 % of the total fatty acids are n-3 fatty acids, preferably more than 23 %, most preferably 24%.
  • the composition comprises more than 23% n-3 fatty acids and less than 1 1 % n-6 FA of the total fatty acids.
  • the content of eicosapentaenoic acid 20:5n-3 is more than 7 % of the total fatty acids, preferably more than 9 %, most preferably 10.2%.
  • Figure 2 shows the modelled cumulative probabilities of myocardiac scores between feeds at 10, 12, 14 and 16 weeks.
  • the graph gives the probability of the myocardiac score being equal or lower than the one read on the x-axis.
  • Figure 3 shows the model coefficients ( ⁇ 95% credible intervals) of the myocardiac score model comparable between feeds at 10, 12, 14 and 16 weeks post-challenge. That means that if the 95% credible interval do not overlap the red dashed line denoting the coefficient of control diet for a particular week, the difference can be considered significant at 5% level. Also, if the feeds are compared across the subplots, the weeks that do not have the 95% credible intervals crossing the zero line (not drawn) can be considered significantly different from the 10 weeks.
  • Figure 4 shows the modelled cumulative probabilities of the epicard scores for each feed at 10, 12, 14 and 16 weeks.
  • the graph gives the probability of the epicardiac score being equal or lower than the one read on the x-axis. Even though differences were not significant between the different diets on epicard scores, there is a clear trend that the epicard scores are improved by feeding of anti-HSMI 1 or 2.
  • Figure 5 shows the model coefficients ( ⁇ 95% credible intervals) of the epicardiac score model comparable between feeds at 10, 12, 14 and 16 weeks post-challenge. That means that if the 95% credible interval do not overlap the red dashed line denoting the coefficient of control diet for a particular week, the difference can be considered significant at 5% level. Also, if the control feeds are compared across the subplots, the weeks that do not have the 95% credible intervals crossing the zero line (not drawn) can be considered significantly different from the 0 weeks.
  • Figure 6 shows the modelled cumulative probabilities of liver score between feeds within sampling weeks.
  • the graph gives the probability of the score being equal or lower than the one read on the x-axis.
  • Figure 7 shows model coefficients ( ⁇ 95% credible intervals) of the liver score model comparable between feeds at 10, 12, 14 and 16 weeks post-challenge. That means that if the 95% credible interval do not overlap the red dashed line denoting the coefficient of control diet for a particular week, the difference can be considered significant at 5% level. Also, if the control feeds are compared across the subplots, the weeks that do not have the 95% credible intervals crossing the zero line (not drawn) can be considered significantly different from the 10 weeks.
  • Figure 8 shows the average weight gain and 95% confidence interval during the 8- week feeding period pre-challenge.
  • Figure 9 shows the level of EPA in heart tissue of Atlantic salmon 8, 10, 12 and 16 weeks post-challenge.
  • the dietary effect on HSMI was tested in a feeding experiment with infected Atlantic salmon ⁇ Salmo salar).
  • the salmon smolts used for the experiment had all been fed the same commercial diet for a period of 10 weeks during the acclimatization period. All the fish were in good condition and showed good and rapid adaptation to sea- water.
  • the trial consisted of 2 phases. Duration of the first part was an 8-week period pre- challenge, which was conducted at a feeding trial facility. After this period the fish were transferred to a disease challenge facility and fish were acclimatized for 2 weeks. After this period the second part of the study comprising challenge, feeding post-challenge lasted for 16 weeks (i.e. 16 weeks post-challenge).
  • a total of 945 Atlantic salmon Salmo salar, pit-tagged, average start weight 110g, seawater adapted were distributed in 9 tanks (1.0 x 1.0 m., 50 cm depth; 500 litre volume) at the feeding trial facility.
  • Three diets were randomly allocated among the 9 tanks (3 tanks per diet) and fed to the fish for a period of 8 weeks. The amount of feed offered during the 8 week period was based on measured or estimated biomass in the tanks.
  • the daily feed rations offered varied between 0.8 - 1.1 % fish body weight per day.
  • Trial feeds given were adjusted through the trial to compensate for fish growth. Fish were kept in seawater with ambient temperature and salinity and diurnal photoperiod, 24:0 (light (L): dark (D)).
  • Water temperature and salinity were measured every day in a representative tank. After the 8-week period, fish were weighed and length-measured, pit-tag recorded and transferred to the disease challenge facility. Once at the challenge facility, the fish were allocated to 18 tanks (2 labs x 9 tanks, i.e. 6 tanks per diet) keeping the same dietary history as during the previous 8 weeks. Fish were left to get acclimatized to the tanks at the challenge facility for 2 weeks and then challenged against HSMI. Fish were anaesthetized prior to challenge to the HSMI virus. The HSMI virus was isolated from sick fish during a clinical outbreak of HSMI.
  • the pellet size of all diets was 3.5 mm.
  • the diets comprised conventional feed ingredients which are commonly comprised in fish feed such as proteins, lipids, carbohydrates, vitamins, minerals.
  • the compositions of the diets were as follows (Table 1 ):
  • Table 1 Moisture, protein, lipid, ash content (all weight % of diet) as well as fatty acid (FA) composition (% of total fatty acids) and n-3/n-6 fatty acid ratio of the diets used in the feeding experiment.
  • Table 2 Lipid classes (%) in the diets fed during the feeding experiment.
  • the histological changes have been evaluated on the basis of a predetermined categorization of changes in the heart.
  • the focus was on the cardiac inflammation:
  • Table 3 Inflammation scores for the epicard and myocard of HSMI infected Atlantic salmon.
  • Score 0 No pathological changes Score 0: No pathological changes observed. observed.
  • Score 1 Focal / multifocal (2-4 foci) of Score 1 : Vascular changes in the small inflammatory cells lifting the epicardial vessels of the compact layer characterized layer from the surface of the heart, by enlarged endothelial cells, typically typically 2-3 cell layers thick. Limited stretching out. No involvement of compact number (countable) of mononuclear layer extending into the compact layer of inflammatory cells infiltrating the the ventricle.
  • Score 2 Diffuse infiltration of Score 2: Focal to multifocal inflammatory inflammatory cells (mononuclear) >5 foci (2-5 foci) of the compact layer and/or in cell layers thick in most of the epicard the spongious part (2-5 foci). Extension present. The infiltration of cells is typically seen along small vessels and multifocal to diffuse and can involve perivascular infiltration,
  • Score 3 Diffuse infiltration of Score 3: The changes in the compact layer inflammatory cells (mononuclear) >10 are multifocal or diffuse in areas and cell layers thick in most of the epicard typically concentrate along small blood present. Moderate pathological vessels. Combined with focal or multifocal changes consisting of high number changes in the spongious layer.
  • Score 4 Diffusely thickened (>15 cell Score 4: Widespread to diffuse infiltration layers) epicard in more than 3 ⁇ 4 of the of inflammatory cells in the compact layer layer present. Severe pathological and involving the spongious layer in a changes characterized by intense multifocal pattern. Degeneration and or infiltration of inflammatory cells in the necrosis of muscle fibers may be/are seen, epicardium, Atrium can also be involved with
  • Liver histopathology was studied by changes related to micro- and macrovesicular steatosis. This is characterized by varying degrees of vacuole-formation in the cytoplasm of the hepatocytes. Score grading followed a non-continuous score from 0 to 5, being 5 the score indicating the highest degree of steatosis of the liver (fatty liver). A description of each of the grading scores is as follows.
  • Histopathological scores were analysed by using a multilevel ordered categorical logistic regression because the data are multinomial.
  • Vague non-informative uniform priors [0,100] were given for the variance parameters, and vague non-informative normal priors N(0, 1.0E+4) for all other parameters.
  • 25000 "burn-in" simulation runs were used to adapt the Markov Chain Monte Carlo (MCMC) before subsequent 2500 runs that were used for inference.
  • Three chains were run in parallel, i.e. there were a total of 7500 simulations for inference. These were thinned so that only every 10th simulation was saved to reduce the size of saved objects and to reduce the effects of
  • livers are yellowish, showing an increased fat content (commonly known and hepatic steatosis) and sometimes necrotic foci are observed where cells are vacuolated and pyknotic.
  • Feeding of the new dietary compositions to HSMI infected Atlantic salmon surprisingly also affected the pathological status of the liver ( Figures 6-7).
  • anti-HSMI 1 and anti-HSMI 2 had clearly higher probabilities of having low scores (i.e. score ⁇ 1 )(79% and 72% respectively) compared to 16% for the control diet fish ( Figure 6a).
  • Figure 9 shows that the level of the fatty acid eicosapentaenoic acid (EPA 20:5n-3) accumulates in the heart tissue, and without being bound by any mechanism it is believed that this increased level of EPA at the right place in the fish body, i.e. in the heart tissue, is favorable for the HSMI-infected fish.
  • EPA 20:5n-3 fatty acid eicosapentaenoic acid
  • South American fish oil which contains approximately double EPA than North Atlantic fish oil, is used to increase the content of EPA in the anti-HSMI diets.
  • n-6 fatty acids typically found in vegetable oils
  • results indicate that a reduced n-3/n-6 ratio such as being in the range of 2.3-3.4 can be beneficial.
  • the liver of HSMI infected fish is affected by the disease as described above. This can have an impact on lipid digestibility in the fish.
  • vegetable oils are added to the diets in a hydrolysed form. It also seems to be an advantage to include hydrolysed phospholipids to the diet. It can also be beneficial to add krill meal and/or krill oil to the diet.
  • the diet may further comprise nucleotides, anti-inflammatory compounds and/or manna- oligosaccharides (MOS). It is assumed that the feed composition according to the present invention is also relevant for other teleost species than salmonids which can be affected by HSMI.
  • n-3 fatty acids are a family of unsaturated fatty acids that have in common a final carbon-carbon double bond in the n-3 position, which is the third bond from the methyl end of the fatty acid. These fatty acids are also known as omega 3 fatty acids.
  • n-6 fatty acids are a family of unsaturated fatty acids that have in common a final carbon-carbon double bond in the n-6 position, which is the sixth bond from the methyl end of the fatty acid. These fatty acids are also known as omega 6 fatty acids.
  • n-3/n-6 ratio is meant the ratio between fatty acids belonging to the n-3 family and those belonging to the n-6 family in the composition or diet.
  • Protein/lipid ratio means the ratio between proteins and lipids comprised in the composition or diet (each of them related to the weight of the diet).
  • HSMI Heart and skeletal muscle inflammation disease
  • conventional feed ingredients refers to ingredients which are commonly in fish feeds such as proteins, lipids, carbohydrates, vitamins, minerals, etc.
  • the ingredients can be derived from marine, vegetable, animal by-products and/ or any other relevant sources and can be used in any suitable combination.
  • nucleotides refer to molecules comprising a nitrogenous base (purine or pyrimidine base), a five-carbon sugar and one to three phosphate groups.

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  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Food Science & Technology (AREA)
  • Marine Sciences & Fisheries (AREA)
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  • Insects & Arthropods (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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Abstract

La présente invention concerne une composition d'aliment qui est appropriée pour nourrir des poissons afin d'empêcher ou de réduire les symptômes relatifs à l'inflammation des muscles cardiaque et squelettiques (HSMI) des poissons.
PCT/NO2010/000336 2009-09-14 2010-09-14 Composition d'aliment Ceased WO2011031166A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
NO20120303A NO347704B1 (no) 2009-09-14 2010-09-14 Fôrsammensetning for fisk for anvendelse i å hindre eller redusere symptomer relatert til hjerte- og skjelettmuskelsykdom, HSMB.
NO20120594A NO20120594A1 (no) 2009-09-14 2012-05-22 Fôrsammensetning for fisk for anvendelse i å hindre eller redusere symptomer relatert til hjerte- og skjelettmuskelbetennelsessykdom
NO20231264A NO348543B1 (no) 2009-09-14 2023-11-21 Fish feed

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NO20093000A NO341929B1 (no) 2009-09-14 2009-09-14 Fôrsammensetning
NO20093000 2009-09-14

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WO2011031166A2 true WO2011031166A2 (fr) 2011-03-17
WO2011031166A3 WO2011031166A3 (fr) 2011-07-28

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WO2014070020A1 (fr) * 2012-11-01 2014-05-08 Ewos Innovation As Composition d'aliment pour les poissons
WO2015110977A1 (fr) * 2014-01-22 2015-07-30 Rolexi Marketing (Pty) Ltd Composition d'acides gras et utilisation médicinale de celle-ci
US20170049824A1 (en) * 2015-08-17 2017-02-23 Aker Biomarine Antarctic As Methods for improving health of farmed fish
US11419350B2 (en) 2016-07-01 2022-08-23 Corbion Biotech, Inc. Feed ingredients comprising lysed microbial cells
WO2023148154A1 (fr) * 2022-02-01 2023-08-10 Dsm Ip Assets B.V. Aliment pour l'aquaculture

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