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WO2011028494A3 - Détection sans marqueur de complexes apn-adn à l'aide de nanopores - Google Patents

Détection sans marqueur de complexes apn-adn à l'aide de nanopores Download PDF

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Publication number
WO2011028494A3
WO2011028494A3 PCT/US2010/046403 US2010046403W WO2011028494A3 WO 2011028494 A3 WO2011028494 A3 WO 2011028494A3 US 2010046403 W US2010046403 W US 2010046403W WO 2011028494 A3 WO2011028494 A3 WO 2011028494A3
Authority
WO
WIPO (PCT)
Prior art keywords
pna
pathogens
nanopores
label
viruses
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2010/046403
Other languages
English (en)
Other versions
WO2011028494A2 (fr
Inventor
Amit Meller
Maxim Frank-Kamenetskii
Meni Wanunu
Heiko Kuhn
Alon Singer
Will Morrison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boston University
Original Assignee
Boston University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boston University filed Critical Boston University
Priority to US13/391,695 priority Critical patent/US20120276530A1/en
Publication of WO2011028494A2 publication Critical patent/WO2011028494A2/fr
Publication of WO2011028494A3 publication Critical patent/WO2011028494A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Des modes de réalisation selon l'invention concernent un procédé de détection de séquences d'ADN spécifiques et l'application de ce procédé pour la détection de pathogènes, de virus, de pathogènes résistant aux médicaments, de variations génomiques associées à une prédisposition à une maladie/trouble etc. en fonction de séquences signatures spécifiques uniques auxdits pathogènes, virus, pathogènes résistant aux médicaments, ou auxdites variations génomiques. Ce procédé peut également être utilisé pour distinguer un ensemble d'ADN bicaténaire de même taille sur la base de différences séquentielles. Ce procédé utilise des sondes d'APN gamma et/ou d'APN-bis marquées de manière non optique pour marquer des séquences cibles spécifiques pour une identification par nanopores à l'état solide.
PCT/US2010/046403 2009-08-24 2010-08-24 Détection sans marqueur de complexes apn-adn à l'aide de nanopores Ceased WO2011028494A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/391,695 US20120276530A1 (en) 2009-08-24 2010-08-24 Label-free sensing of pna-dna complexes using nanopores

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23618709P 2009-08-24 2009-08-24
US61/236,187 2009-08-24

Publications (2)

Publication Number Publication Date
WO2011028494A2 WO2011028494A2 (fr) 2011-03-10
WO2011028494A3 true WO2011028494A3 (fr) 2011-06-16

Family

ID=43649883

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/046403 Ceased WO2011028494A2 (fr) 2009-08-24 2010-08-24 Détection sans marqueur de complexes apn-adn à l'aide de nanopores

Country Status (2)

Country Link
US (1) US20120276530A1 (fr)
WO (1) WO2011028494A2 (fr)

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JP6016792B2 (ja) 2010-07-14 2016-10-26 ザ キュレイターズ オブ ザ ユニバーシティ オブ ミズーリ ナノポア促進型の核酸の単分子検出
CN102507395A (zh) * 2011-10-13 2012-06-20 东南大学 实时监测溶液中病毒颗粒的方法
EP2780480B1 (fr) * 2011-11-14 2018-10-03 Carnegie Mellon University Minisondes gamma-pna pour un marquage fluorescent
WO2014144217A1 (fr) 2013-03-15 2014-09-18 The Curators Of The University Of Missouri Capteur à nanopores codés pour la détection multiplexée d'acides nucléiques
KR102245192B1 (ko) * 2013-05-06 2021-04-29 온테라 인크. 나노포어를 이용한 표적 검출
US20140329225A1 (en) 2013-05-06 2014-11-06 Two Pore Guys, Inc. Target detection with nanopore
WO2014210219A1 (fr) * 2013-06-25 2014-12-31 Two Pore Guys, Inc. Quantification de biomarqueurs multiplexés par l'analyse de nanopores de complexes biomarqueur-polymère
US20150037787A1 (en) * 2013-07-31 2015-02-05 International Business Machines Corporation Polynucleotide configuration for reliable electrical and optical sensing
US10745735B2 (en) 2013-11-22 2020-08-18 National Research Council Of Canada Detection, isolation and identification of microorganisms
CN104032032B (zh) * 2014-07-07 2016-04-13 玉峰惠仁生物医药科技（北京）有限公司 用于检测铜绿假单胞菌、肺炎克雷伯氏菌和/或鲍曼不动杆菌的肽核酸探针组及其试剂盒
US11414712B2 (en) * 2014-09-17 2022-08-16 Helixbind, Inc. Methods and devices for detecting and identifying microorganisms
CN107002140A (zh) * 2014-09-26 2017-08-01 双孔人公司 通过合成探针的纳米孔隙探测的靶序列检测
US11124822B2 (en) * 2014-10-17 2021-09-21 Carnegie Mellon University Enhanced biomolecule detection assays based on tyramide signal amplification and gammaPNA probes
CN109414695B (zh) 2016-03-14 2022-03-18 海利克斯拜恩德股份有限公司 集成流体装置及相关方法
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
US11840721B2 (en) 2017-04-03 2023-12-12 Helixbind, Inc. Methods and devices for identifying microbial infections
JP2021165635A (ja) * 2018-04-27 2021-10-14 株式会社Ｓｃｒｅｅｎホールディングス 配列決定方法および配列決定装置
US11713330B2 (en) 2019-04-16 2023-08-01 Helixbind, Inc. Methods and devices for ultrasensitive direct detection of microorganisms
CN116814818A (zh) * 2019-07-22 2023-09-29 四川大学华西医院 一种区分抗生素耐药的肺炎克雷伯菌的装置及方法
WO2021086134A1 (fr) * 2019-11-01 2021-05-06 한국생명공학연구원 Procédé et kit de détection pour la détection de micro-organismes pathogènes à l'aide de nanopores

Citations (2)

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Publication number Priority date Publication date Assignee Title
US6265166B1 (en) * 1998-04-29 2001-07-24 Trustees Of Boston University Methods and compositions pertaining to PD-loops
US6617113B2 (en) * 1999-09-07 2003-09-09 The Regents Of The University Of California Methods of determining the presence of double stranded nucleic acids in a sample

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EP1954838B1 (fr) * 2005-11-14 2014-02-26 Life Technologies Corporation Molecules codees permettant de detecter des substances cibles a analyser
EP2411536B1 (fr) * 2009-03-27 2014-09-17 Nabsys, Inc. Procédés d'analyse de biomolécules et des sondes liées à celles-ci

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265166B1 (en) * 1998-04-29 2001-07-24 Trustees Of Boston University Methods and compositions pertaining to PD-loops
US6617113B2 (en) * 1999-09-07 2003-09-09 The Regents Of The University Of California Methods of determining the presence of double stranded nucleic acids in a sample

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
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RAPIREDDY, S. ET AL.: "Strand invasion of mixed-sequence B-DNA by acridine-linked, gamma-peptide nucleic acid (gamma-PNA)", J. AM. CHEM. SOC., vol. 129, no. 50, 19 December 2007 (2007-12-19), pages 15596 - 15600 *
SINGER, A. ET AL.: "Nanopore based sequence specific detection of duplex DNA for genomic profiling", NANO LETT., vol. 10, no. 2, 10 February 2010 (2010-02-10), pages 738 - 742 *

Also Published As

Publication number Publication date
WO2011028494A2 (fr) 2011-03-10
US20120276530A1 (en) 2012-11-01

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