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WO2011014673A1 - Cassette d’immunoessai à flux latéral automatisé avec propriétés d’écoulement améliorées - Google Patents

Cassette d’immunoessai à flux latéral automatisé avec propriétés d’écoulement améliorées Download PDF

Info

Publication number
WO2011014673A1
WO2011014673A1 PCT/US2010/043742 US2010043742W WO2011014673A1 WO 2011014673 A1 WO2011014673 A1 WO 2011014673A1 US 2010043742 W US2010043742 W US 2010043742W WO 2011014673 A1 WO2011014673 A1 WO 2011014673A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
transport element
conjugate pad
analyte
fluid transport
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2010/043742
Other languages
English (en)
Inventor
William H. Chapman, Jr.
Richard K.T. Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cholestech Corp
Original Assignee
Cholestech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cholestech Corp filed Critical Cholestech Corp
Priority to CN201080032391.5A priority Critical patent/CN102472751B/zh
Publication of WO2011014673A1 publication Critical patent/WO2011014673A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow

Definitions

  • the present invention relates to systems and methods for conducting assays, including qualitative, semi-quantitative and quantitative determinations of one or more analytes using a lateral flow assay format.
  • the conjugate pad engages and restricts flow of fluid through a region of the first fluid transport element, thereby providing a flow path such that fluid flows through the conjugate pad to an uncompressed region of the first fluid transport element downstream of said conjugate pad, and wherein the second fluid transport element, which is in fluid communication with this uncompressed region of the first fluid transport element receives fluid therefrom;
  • the receptors to the one or more analytes of interest can be conjugated to an enzyme, fluorophore or other detectable label. Enzyme conjugates are among the most common conjugates used.
  • direct label refers to a signal development element from which a signal can be generated without the addition of a further binding molecule that specifically binds one or more components of the analyte/receptor complex being detected.
  • direct labels include enzyme labels, fluorescent labels, electrochemical labels, metal chelates, colloidal metal labels, and biosensors relying on optical detection such as surface plasmon resonance and ellipsometry.
  • indirect label refers to a signal development element that binds, not to the analyte, but to a molecule that is itself bound to the analyte.
  • a labeled secondary antibody for example a detectably labeled goat anti-mouse IgG that binds to a mouse antibody directed to the analyte of interest, is an example of an indirect label.
  • linkage chemistries often provide the molecule of interest with one or more functional groups that couple to amino acid side chains of peptides.
  • these "linkage reagents” may be classified on the basis of the following: 1. Functional group(s) and chemical specificity;

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne des procédés, des dispositifs, et des instruments pour effectuer des essais de liaison de récepteur. En particulier, les dispositifs présentement décrits permettent un processus de mouillage initial pour redissoudre des réactifs d’essai dans une cartouche de conjugué avant mobilisation de ces réactifs d’essai dans un élément à flux latéral. Ce mouillage initial se produit de préférence le long d’une surface plane de la cartouche de conjugué ayant la plus grande dimension.
PCT/US2010/043742 2009-07-31 2010-07-29 Cassette d’immunoessai à flux latéral automatisé avec propriétés d’écoulement améliorées Ceased WO2011014673A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201080032391.5A CN102472751B (zh) 2009-07-31 2010-07-29 带有改良流体结构的自动化免疫横向流动测试盒

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23023109P 2009-07-31 2009-07-31
US61/230,231 2009-07-31

Publications (1)

Publication Number Publication Date
WO2011014673A1 true WO2011014673A1 (fr) 2011-02-03

Family

ID=43529699

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/043742 Ceased WO2011014673A1 (fr) 2009-07-31 2010-07-29 Cassette d’immunoessai à flux latéral automatisé avec propriétés d’écoulement améliorées

Country Status (2)

Country Link
CN (1) CN102472751B (fr)
WO (1) WO2011014673A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3029462A4 (fr) * 2013-08-02 2017-03-22 Denka Seiken Co., Ltd. Procédé d'amplification de lumière de détection à l'aide d'un matériau réfléchissant la lumière en immunochromatographie
US10688487B2 (en) 2016-11-23 2020-06-23 Bio-Rad Laboratories, Inc. Lateral flow assay device
US10883987B2 (en) 2017-01-27 2021-01-05 Bio-Rad Laboratories, Inc. Lateral flow device
US11175284B2 (en) 2017-11-28 2021-11-16 Bio-Rad Laboratories, Inc. Lateral flow assay device
US11980883B2 (en) 2017-08-25 2024-05-14 Bio-Rad Laboratories, Inc. Lateral flow pump housing

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103018439B (zh) * 2012-11-30 2013-12-25 中国农业科学院油料作物研究所 一种提高胶体金免疫层析试纸条检测灵敏度的方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030215358A1 (en) * 2002-01-15 2003-11-20 Schulman Lloyd S. Liquid permeable composition in dry reagent devices
US20050147532A1 (en) * 2002-06-07 2005-07-07 Bellet Neal F. Automated immunoassay cassette, apparatus and method
US20050227371A1 (en) * 2004-03-23 2005-10-13 Quidel Corporation Hybrid phase lateral flow assay
US20060189858A1 (en) * 2005-02-14 2006-08-24 Sterling Bernhard B Analyte detection system for multiple analytes
US20080166745A1 (en) * 2007-01-09 2008-07-10 Cholestech Corporation Device and method for measuring LDL-associated cholesterol

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005098439A2 (fr) * 2004-03-30 2005-10-20 Whatman, Inc. Et format d'ecoulement lateral, materiaux et procedes
CN101031798B (zh) * 2004-07-29 2012-06-27 瑞莱诊断体系有限公司 侧向流系统和测定

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030215358A1 (en) * 2002-01-15 2003-11-20 Schulman Lloyd S. Liquid permeable composition in dry reagent devices
US20050147532A1 (en) * 2002-06-07 2005-07-07 Bellet Neal F. Automated immunoassay cassette, apparatus and method
US20050227371A1 (en) * 2004-03-23 2005-10-13 Quidel Corporation Hybrid phase lateral flow assay
US20060189858A1 (en) * 2005-02-14 2006-08-24 Sterling Bernhard B Analyte detection system for multiple analytes
US20080166745A1 (en) * 2007-01-09 2008-07-10 Cholestech Corporation Device and method for measuring LDL-associated cholesterol

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3029462A4 (fr) * 2013-08-02 2017-03-22 Denka Seiken Co., Ltd. Procédé d'amplification de lumière de détection à l'aide d'un matériau réfléchissant la lumière en immunochromatographie
US10345294B2 (en) 2013-08-02 2019-07-09 Denka Seiken Co., Ltd. Method of amplifying detection light using light-reflecting material in immunochromatography
US11371987B2 (en) 2013-08-02 2022-06-28 Denka Company Limited Method of amplifying detection light using light-reflecting material, in immunochromatography
US10688487B2 (en) 2016-11-23 2020-06-23 Bio-Rad Laboratories, Inc. Lateral flow assay device
US11471882B2 (en) 2016-11-23 2022-10-18 Bio-Rad Laboratories, Inc. Lateral flow assay device
US11826753B2 (en) 2016-11-23 2023-11-28 Bio-Rad Laboratories, Inc. Lateral flow assay device
US10883987B2 (en) 2017-01-27 2021-01-05 Bio-Rad Laboratories, Inc. Lateral flow device
US11980883B2 (en) 2017-08-25 2024-05-14 Bio-Rad Laboratories, Inc. Lateral flow pump housing
US11175284B2 (en) 2017-11-28 2021-11-16 Bio-Rad Laboratories, Inc. Lateral flow assay device

Also Published As

Publication number Publication date
CN102472751A (zh) 2012-05-23
CN102472751B (zh) 2014-10-22

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