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WO2011002522A3 - Methods of making and using synthetic viruses - Google Patents

Methods of making and using synthetic viruses Download PDF

Info

Publication number
WO2011002522A3
WO2011002522A3 PCT/US2010/001895 US2010001895W WO2011002522A3 WO 2011002522 A3 WO2011002522 A3 WO 2011002522A3 US 2010001895 W US2010001895 W US 2010001895W WO 2011002522 A3 WO2011002522 A3 WO 2011002522A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
synthetic viruses
membrane
making
aperture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2010/001895
Other languages
French (fr)
Other versions
WO2011002522A2 (en
Inventor
Philippe-Alexandre Gilbert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MedImmune LLC
Original Assignee
MedImmune LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MedImmune LLC filed Critical MedImmune LLC
Publication of WO2011002522A2 publication Critical patent/WO2011002522A2/en
Publication of WO2011002522A3 publication Critical patent/WO2011002522A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/00051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/00061—Methods of inactivation or attenuation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)

Abstract

This invention provides synthetic viruses, and methods and devices to prepare synthetic viruses. In the methods, a lipid bilayer membrane is established at an aperture between tow compartments. Fluids contact the membrane to deliver proteins and a nucleic acid core to the membrane before budding. The devices include a lipid bilayer across an aperture with an affinity protein on one side and a nucleic acid core bound to the other side.
PCT/US2010/001895 2009-07-02 2010-07-02 Methods of making and using synthetic viruses Ceased WO2011002522A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22257009P 2009-07-02 2009-07-02
US61/222,570 2009-07-02

Publications (2)

Publication Number Publication Date
WO2011002522A2 WO2011002522A2 (en) 2011-01-06
WO2011002522A3 true WO2011002522A3 (en) 2011-05-26

Family

ID=43411653

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/001895 Ceased WO2011002522A2 (en) 2009-07-02 2010-07-02 Methods of making and using synthetic viruses

Country Status (1)

Country Link
WO (1) WO2011002522A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9504743B2 (en) 2013-09-25 2016-11-29 Sequoia Sciences, Inc Compositions of vaccines and adjuvants and methods for the treatment of urinary tract infections
US9149521B2 (en) 2013-09-25 2015-10-06 Sequoia Sciences, Inc. Compositions of vaccines and adjuvants and methods for the treatment of urinary tract infections
US9149522B2 (en) 2013-09-25 2015-10-06 Sequoia Sciences, Inc. Compositions of vaccines and adjuvants and methods for the treatment of urinary tract infections
US20150086592A1 (en) 2013-09-25 2015-03-26 Sequoia Sciences, Inc Compositions of vaccines and adjuvants and methods for the treatment of urinary tract infections
AU2020321021A1 (en) * 2019-07-30 2022-03-10 Verndari, Inc. Virus-like particle vaccines
IT201900013758A1 (en) * 2019-08-01 2021-02-01 Biomimesi Srl Active phospholipid membrane and related production process
CN113267396B (en) * 2021-07-16 2021-09-28 易普森生物科技(深圳)有限公司 Cell film-making dyeing machine

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BUCHER ET AL.: "Incorporation of influenza virus M-protein into liposomes", JOURNAL OF VIROLOGY, vol. 36, no. 2, 30 November 1980 (1980-11-30), pages 586 - 590 *
FRALEY ET AL.: "Introduction of liposome-encapsulated SV40 DNA into cells", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 255, no. 21, 10 November 1980 (1980-11-10), pages 10431 - 10435, XP002007683 *
MACLACHLAN ET AL.: "Progress towards a synthetic virus for systemic gene therapy", CURRENT OPINION IN MOLECULAR THERAPEUTICS, vol. 1, no. 2, 30 April 1999 (1999-04-30), pages 252 - 259 *
RAMANI ET AL.: "Novel gene delivery to liver cells using engineered virosomes", FEBS LETTERS, vol. 404, 10 March 1997 (1997-03-10), pages 164 - 168, XP000942134, DOI: doi:10.1016/S0014-5793(97)00120-8 *

Also Published As

Publication number Publication date
WO2011002522A2 (en) 2011-01-06

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