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WO2011002220A3 - 폴리하이드록시알카노에이트 합성효소 변이체를 이용한 락테이트 중합체 및 락테이트 공중합체의 제조방법 - Google Patents

폴리하이드록시알카노에이트 합성효소 변이체를 이용한 락테이트 중합체 및 락테이트 공중합체의 제조방법 Download PDF

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Publication number
WO2011002220A3
WO2011002220A3 PCT/KR2010/004240 KR2010004240W WO2011002220A3 WO 2011002220 A3 WO2011002220 A3 WO 2011002220A3 KR 2010004240 W KR2010004240 W KR 2010004240W WO 2011002220 A3 WO2011002220 A3 WO 2011002220A3
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WO
WIPO (PCT)
Prior art keywords
lactate
copolymers
synthase
polymers
mutants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2010/004240
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English (en)
French (fr)
Other versions
WO2011002220A2 (ko
WO2011002220A9 (ko
Inventor
양택호
강혜옥
이상엽
정유경
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LG Chem Ltd
Korea Advanced Institute of Science and Technology KAIST
Original Assignee
LG Chem Ltd
Korea Advanced Institute of Science and Technology KAIST
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Publication date
Application filed by LG Chem Ltd, Korea Advanced Institute of Science and Technology KAIST filed Critical LG Chem Ltd
Priority to EP10794355A priority Critical patent/EP2471910A4/en
Priority to JP2012518494A priority patent/JP2012531905A/ja
Priority to US13/381,340 priority patent/US20120171737A1/en
Priority to CN2010800387160A priority patent/CN102575234A/zh
Publication of WO2011002220A2 publication Critical patent/WO2011002220A2/ko
Publication of WO2011002220A3 publication Critical patent/WO2011002220A3/ko
Anticipated expiration legal-status Critical
Publication of WO2011002220A9 publication Critical patent/WO2011002220A9/ko
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/03—CoA-transferases (2.8.3)
    • C12Y208/03001—Propionate CoA-transferase (2.8.3.1)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

본 발명은 락테이트 중합체 (PLA) 및 락테이트 공중합체 (PLA copolymer)를 합성할 수 있는 다양한 폴리하이드록시알카노에이트 합성효소(polyhydroxyalkanoate synthase; PHA synthase)의 변이체들과 그것을 이용한 락테이트 중합체 및 락테이트 공중합체의 제조방법에 관한 것으로, 더욱 자세하게는, 서열번호가 2, 4, 6 또는 8인 폴리하이드록시알카노에이트 합성효소의 변이체, 그리고 상기 합성효소 변이체를 이용하는 것을 특징으로 하는 락테이트 중합체 및 락테이트 공중합체의 제조 방법에 관한 것이다. 본 발명에 따르면, 서열번호가 2, 4, 6 또는 8인 폴리하이드록시알카노에이트 합성효소들은 락테이트 중합체 합성 활성에 영향을 미치는 아미노산 서열 변이에 의해 락테이트 중합체 및 락테이트 공중합체 합성 활성을 가질 수 있게 되며, 이들 합성효소 변이체들을 이용하면 각기 다른 성질을 가지는 락테이트 중합체 및 공중합체를 제조할 수 있다.
PCT/KR2010/004240 2009-06-30 2010-06-30 폴리하이드록시알카노에이트 합성효소 변이체를 이용한 락테이트 중합체 및 락테이트 공중합체의 제조방법 Ceased WO2011002220A2 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP10794355A EP2471910A4 (en) 2009-06-30 2010-06-30 METHOD FOR PRODUCING LACTATE POLYMERS AND LACTAT COPOLYMERS USING POLYHYDROXYALKANOATE SYNTHESIS MUTANES
JP2012518494A JP2012531905A (ja) 2009-06-30 2010-06-30 ポリヒドロキシアルカノエート合成酵素変異体を利用したラクテート重合体及びラクテート共重合体の製造方法
US13/381,340 US20120171737A1 (en) 2009-06-30 2010-06-30 Preparation method of lactate polymers and lactate copolymers using polyhydroxyalkanoate synthase mutants
CN2010800387160A CN102575234A (zh) 2009-06-30 2010-06-30 使用多羟基链烷酸酯合酶突变体制备乳酸酯聚合物和乳酸酯共聚物的方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090059488A KR101211767B1 (ko) 2009-06-30 2009-06-30 폴리하이드록시알카노에이트 합성효소 변이체를 이용한 락테이트 중합체 및 락테이트 공중합체의 제조방법
KR10-2009-0059488 2009-06-30

Publications (3)

Publication Number Publication Date
WO2011002220A2 WO2011002220A2 (ko) 2011-01-06
WO2011002220A3 true WO2011002220A3 (ko) 2011-05-19
WO2011002220A9 WO2011002220A9 (ko) 2013-07-04

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PCT/KR2010/004240 Ceased WO2011002220A2 (ko) 2009-06-30 2010-06-30 폴리하이드록시알카노에이트 합성효소 변이체를 이용한 락테이트 중합체 및 락테이트 공중합체의 제조방법

Country Status (6)

Country Link
US (1) US20120171737A1 (ko)
EP (2) EP2650372B1 (ko)
JP (1) JP2012531905A (ko)
KR (1) KR101211767B1 (ko)
CN (1) CN102575234A (ko)
WO (1) WO2011002220A2 (ko)

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JP5637711B2 (ja) * 2010-03-25 2014-12-10 トヨタ自動車株式会社 組み換え微生物及びこれを用いた脂肪族ポリエステルの製造方法
EP4056631A1 (en) 2014-05-16 2022-09-14 Carbios Process of recycling mixed plastic articles
MA40138B1 (fr) 2014-10-21 2018-09-28 Agronomique Inst Nat Rech Polypeptide présentant une activité de dégradation de polyester et utilisations de celui-ci
US10626242B2 (en) 2014-12-19 2020-04-21 Carbios Plastic compound and preparation process
JP6478697B2 (ja) * 2015-02-24 2019-03-06 国立大学法人東京工業大学 高結晶化速度を有する微生物ポリエステル共重合体及びその製造法
EP3268469A1 (en) 2015-03-13 2018-01-17 Carbios New polypeptide having a polyester degrading activity and uses thereof
PT3307812T (pt) 2015-06-12 2024-07-01 Carbios Composição biodegradável de poliéster e suas utilizações
KR102002096B1 (ko) * 2015-08-12 2019-10-01 주식회사 엘지화학 4-하이드록시부티레이트, 2-하이드록시부티레이트 및 락테이트를 반복단위로 포함하는 삼중합체 및 이의 제조방법
RU2758138C2 (ru) * 2015-12-21 2021-10-26 Карбио Рекомбинантные клетки дрожжей, продуцирующие полимолочную кислоту, и их использования
KR101774431B1 (ko) 2016-01-28 2017-09-05 한국과학기술원 자일로즈로부터 폴리(락테이트-co-글라이콜레이트) 또는 그 공중합체 생산능을 가지는 재조합 미생물 및 이를 이용한 폴리(락테이트-co-글라이콜레이트) 또는 그 공중합체의 제조방법
MX2018014090A (es) 2016-05-19 2019-04-01 Carbios Un proceso para degradar productos de plastico.
EP3299424A1 (en) * 2016-09-22 2018-03-28 Cappelle Pigments nv Method for manufacturing bismuth based pigment having an improved alkaline resistance by encapsulating said pigment with a chelating agent
KR102318930B1 (ko) 2017-12-18 2021-10-27 주식회사 엘지화학 폴리하이드록시알카노에이트를 포함하는 접착제 조성물
KR102497785B1 (ko) 2018-01-08 2023-02-07 주식회사 엘지화학 2-하이드록시부티레이트 및 락테이트의 공중합체 제조를 위한 재조합 균주 및 방법
KR102361618B1 (ko) 2018-01-08 2022-02-09 주식회사 엘지화학 2-하이드록시부티레이트 및 3-하이드록시부티레이트의 공중합체 및 이의 제조방법
KR20190090602A (ko) 2018-01-25 2019-08-02 주식회사 엘지화학 2-하이드록시부티레이트, 2-하이드록시이소발레레이트 및 락테이트의 삼중합체 제조를 위한 재조합 균주 및 방법
KR102538109B1 (ko) 2018-02-02 2023-05-26 주식회사 엘지화학 ldhD 유전자가 도입된 재조합 미생물을 이용한 폴리락트산 단일중합체의 제조 방법
CN114196609B (zh) * 2021-12-31 2023-06-23 淮阴工学院 从乳酸合成纯聚乳酸的大肠杆菌工程菌及其制备方法和应用

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MATSUMOTO, K. ET AL.: "In vivo and In vitro characterization of Ser477X mutations in polyhydroxyalkanoate (PHA) sunthase 1 from Pseudomonas sp. 61-3: effects of beneficial mutations on enzymatic activity, substrate specificity, dnd molecular weight of PHA", BIOMACROMOLECULES., vol. 7, no. 8, August 2006 (2006-08-01), pages 2436 - 2442 *
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Also Published As

Publication number Publication date
JP2012531905A (ja) 2012-12-13
WO2011002220A2 (ko) 2011-01-06
EP2471910A2 (en) 2012-07-04
EP2650372A2 (en) 2013-10-16
EP2650372B1 (en) 2017-03-01
WO2011002220A9 (ko) 2013-07-04
US20120171737A1 (en) 2012-07-05
KR101211767B1 (ko) 2012-12-12
EP2650372A3 (en) 2014-04-30
CN102575234A (zh) 2012-07-11
EP2471910A4 (en) 2013-03-20
KR20110001789A (ko) 2011-01-06

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